mRNA_L-elsbetiae_contig10653.623.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig10653.623.1
Unique NamemRNA_L-elsbetiae_contig10653.623.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: D7G4Y8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G4Y8_ECTSI)

HSP 1 Score: 145 bits (367), Expect = 8.010e-38
Identity = 70/129 (54.26%), Postives = 96/129 (74.42%), Query Frame = 1
Query:    1 DGILRDLAYVSAGNSKPERTARSKRKGVVVGAFPNPLAMVVYDPVQQTGMDIIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARRFLPPNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIF 387
            DG+  DLA V++   K E    S+  GVVV  +PNPL  V +D V+  GMD++HQ +LN  ++I+ +VL AL +AG+ +VR R A RR LPPNV P++DITSAGG+++LTGQ+LW+VSS+FPFLV+PIF
Sbjct:   72 DGLREDLALVASIKKKQEHLKMSRALGVVVPRYPNPLDEVTFDRVEGCGMDVLHQSALNAGKKIMTFVLDALDSAGSEIVRGRFAWRRMLPPNVSPMADITSAGGWAALTGQQLWLVSSIFPFLVAPIF 200          
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: D7FZI4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FZI4_ECTSI)

HSP 1 Score: 145 bits (365), Expect = 6.020e-37
Identity = 69/129 (53.49%), Postives = 97/129 (75.19%), Query Frame = 1
Query:    1 DGILRDLAYVSAGNSKPERTARSKRKGVVVGAFPNPLAMVVYDPVQQTGMDIIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARRFLPPNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIF 387
            DG+  DLA V++   + E+   S+  GVVV  +PNPL  V +D V+  GMD++HQ +LN  ++I+ +VL AL +AG+ +VR R A RR LPPNV P++DITSAGG+++LTGQ+LW+VSS+FPFLV+PIF
Sbjct:  177 DGLREDLALVASIEKRQEQLKMSRALGVVVPRYPNPLDEVTFDRVEGCGMDVLHQSALNAGKKIMTFVLDALDSAGSEIVRGRFAWRRMLPPNVSPMADITSAGGWAALTGQQLWLVSSIFPFLVAPIF 305          
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: D7FWW9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FWW9_ECTSI)

HSP 1 Score: 140 bits (354), Expect = 2.890e-35
Identity = 71/137 (51.82%), Postives = 97/137 (70.80%), Query Frame = 1
Query:    1 DGILRDLAYVSAGNSKPERTARSKRKGVVVGAFPNPLAMVVYDPVQQTGMDIIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARRFLPPNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIFEDAASLVR 411
            DG+  DL+ V+A   K +R   S+  GVVV  +PNP   V +D V+  GMDI+HQ +LN  ++I+ +V+ AL  AG+ +VRAR A  R LPPNV P++DITSAGG+S+LTGQ+LW+VSS FPFL++PIF    +L R
Sbjct:   83 DGLREDLSRVAATEKKQDRDKLSRALGVVVPRYPNPQDEVAFDRVEGCGMDILHQSALNSGKKIMGFVMDALDPAGSEIVRARFAWCRMLPPNVSPMADITSAGGWSALTGQQLWLVSSSFPFLLAPIFSSPDNLGR 219          
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: A0A6H5KPX4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KPX4_9PHAE)

HSP 1 Score: 96.7 bits (239), Expect = 1.690e-20
Identity = 47/90 (52.22%), Postives = 65/90 (72.22%), Query Frame = 1
Query:  139 QTGMDIIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARRFLPPNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIFEDAASLV 408
            Q    +  + +LN  ++I+ +V+ AL   G  VVRAR A RR LPPNV P++DITSAGG S+LTGQ+LW+V+S+FPFL++PIF    +LV
Sbjct:  174 QAACALTGESALNSGKKIMGFVMDALDPTGTEVVRARFAWRRMLPPNVSPIADITSAGGRSALTGQQLWLVASIFPFLLAPIFSSPDNLV 263          
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: A0A6H5K975_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K975_9PHAE)

HSP 1 Score: 97.4 bits (241), Expect = 1.060e-19
Identity = 56/128 (43.75%), Postives = 77/128 (60.16%), Query Frame = 1
Query:   37 GNSK---PERTARSKRKGVVVGAFPNPLAMVVYDPVQQTGMDIIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARRFLPPNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIFEDAASLVR 411
            GN K    +R   S+  GVVV  +PNPL  V +D V+  GMDI+HQ +LN  ++I+ +V+ AL  AG+ +V                  DITSAGG S+LTGQ+LW+VSS+ PFL++PIF    +L R
Sbjct:   30 GNPKYDIKDREKLSRALGVVVPRYPNPLDEVAFDRVEGCGMDILHQSALNSGKKIMGFVMDALDPAGSEIV-----------------PDITSAGGRSALTGQQLWLVSSIVPFLLAPIFSSPDNLGR 140          
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: D7FSA9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FSA9_ECTSI)

HSP 1 Score: 94.7 bits (234), Expect = 1.490e-19
Identity = 44/78 (56.41%), Postives = 63/78 (80.77%), Query Frame = 1
Query:  154 IIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARRFLPPNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIF 387
            ++ Q +LN  ++I+ +VL AL +A + +VR R A RR LP NV P++DITSAGG+++LTGQ+LW+VSS+FPFLV+PIF
Sbjct:    4 LLTQSALNAGKKIMTFVLDALDSAVSEIVRGRFAWRRMLPQNVLPMADITSAGGWAALTGQQLWLVSSIFPFLVAPIF 81          
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: A0A6H5LGI1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LGI1_9PHAE)

HSP 1 Score: 90.5 bits (223), Expect = 2.950e-17
Identity = 44/73 (60.27%), Postives = 59/73 (80.82%), Query Frame = 1
Query:  190 ILAWVLAALMAAGNAVVRARLAARRFLPPNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIFEDAASLV 408
            I+ +V+ AL  AG+ +VRAR A RR LP NV P++DITSAGG+S+LTGQ+LWIVSS+FPFL++P+F    +LV
Sbjct:  605 IMGFVMDALDPAGSDIVRARFAWRRMLPRNVSPIADITSAGGWSALTGQQLWIVSSIFPFLLAPMFSSPDNLV 677          
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: D8LJH2_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LJH2_ECTSI)

HSP 1 Score: 65.1 bits (157), Expect = 1.490e-8
Identity = 48/150 (32.00%), Postives = 70/150 (46.67%), Query Frame = 1
Query:   19 LAYVSAGNSKPERTARSKRKGVVVGAFPNPLAMVVY-DPVQQTGMDIIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARRFLP-PNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIFEDAASLVRRKLESSFTTRNGVGERH 462
            L  +S   +  +R A S + GV      NP    V+ + V+  G+D+ HQD+ N  R+I   +L  L  A    + + LA    L      PL D  S GGFS+L+G ++W +SSV   +  P+    AS+VR         RN    RH
Sbjct:  630 LKVISTAPTGTQRQALSSKHGVTPQEVENPFRKHVHLNMVRTFGIDLFHQDAQNSLRKIFKRLLGGLSKAKGVPLLSCLAKDPSLRVAGTPPLRDFVSDGGFSALSGMDVWTLSSVALLVFRPVLRSVASMVR------LPDRNASRPRH 773          
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: A0A6H5JWC7_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JWC7_9PHAE)

HSP 1 Score: 59.7 bits (143), Expect = 9.080e-7
Identity = 34/88 (38.64%), Postives = 51/88 (57.95%), Query Frame = 1
Query:  148 MDIIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARRFLPPNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIFEDAASLVR 411
            M ++ Q +LN  ++I+ +V+ AL  AG+ +VRAR                   AGG S+LT Q+LW+V S+FPFL++PIF    +L R
Sbjct:    1 MLLLTQSALNSGKKIMGFVMDALDPAGSEIVRAR-------------------AGGGSALTSQQLWLVPSIFPFLLAPIFSSPDNLGR 69          
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Match: D7FWW6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FWW6_ECTSI)

HSP 1 Score: 59.7 bits (143), Expect = 1.020e-6
Identity = 42/133 (31.58%), Postives = 64/133 (48.12%), Query Frame = 1
Query:   19 LAYVSAGNSKPERTARSKRKGVVVGAFPNPLAMVVY-DPVQQTGMDIIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARRFLP-PNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIFEDAASLVR 411
            L  +S   +  +R A S + GV      NP    V+ + V+  G+D+ HQD+ N   +I   +L  L  A    + + LA    L      PL D  S GGFS+L+G ++W +SSV   +  P+    AS+ R
Sbjct:  536 LKVISTATTGTQRQALSSKHGVTPQEVENPFRKHVHLNMVRTFGIDVFHQDAQNSLLKIFKRLLGGLSKAKGVPLLSCLAKDPSLRVAGTPPLRDFVSDGGFSALSGMDVWRLSSVALLVFRPVLRSVASMKR 668          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig10653.623.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 10
Match NameE-valueIdentityDescription
D7G4Y8_ECTSI8.010e-3854.26Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7FZI4_ECTSI6.020e-3753.49Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7FWW9_ECTSI2.890e-3551.82Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KPX4_9PHAE1.690e-2052.22Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5K975_9PHAE1.060e-1943.75Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FSA9_ECTSI1.490e-1956.41Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5LGI1_9PHAE2.950e-1760.27Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LJH2_ECTSI1.490e-832.00Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JWC7_9PHAE9.080e-738.64Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FWW6_ECTSI1.020e-631.58Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig10653contigL-elsbetiae_contig10653:3191..4814 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons2
Model size692
Cds size486
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig10653.623.1prot_L-elsbetiae_contig10653.623.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig10653 3305..4722 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815285.5904253-UTR-L-elsbetiae_contig10653:3190..33041622815285.5904253-UTR-L-elsbetiae_contig10653:3190..3304Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig10653 3191..3304 +
1691682817.2029889-UTR-L-elsbetiae_contig10653:3190..33041691682817.2029889-UTR-L-elsbetiae_contig10653:3190..3304Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig10653 3191..3304 +
1622815285.6247153-UTR-L-elsbetiae_contig10653:4722..48141622815285.6247153-UTR-L-elsbetiae_contig10653:4722..4814Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig10653 4723..4814 +
1691682817.2322872-UTR-L-elsbetiae_contig10653:4722..48141691682817.2322872-UTR-L-elsbetiae_contig10653:4722..4814Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig10653 4723..4814 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815285.6020005-CDS-L-elsbetiae_contig10653:3304..33551622815285.6020005-CDS-L-elsbetiae_contig10653:3304..3355Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig10653 3305..3355 +
1691682817.2137866-CDS-L-elsbetiae_contig10653:3304..33551691682817.2137866-CDS-L-elsbetiae_contig10653:3304..3355Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig10653 3305..3355 +
1622815285.61258-CDS-L-elsbetiae_contig10653:4287..47221622815285.61258-CDS-L-elsbetiae_contig10653:4287..4722Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig10653 4288..4722 +
1691682817.2232826-CDS-L-elsbetiae_contig10653:4287..47221691682817.2232826-CDS-L-elsbetiae_contig10653:4287..4722Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig10653 4288..4722 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig10653.623.1

>prot_L-elsbetiae_contig10653.623.1 ID=prot_L-elsbetiae_contig10653.623.1|Name=mRNA_L-elsbetiae_contig10653.623.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=162bp
MVVYDPVQQTGMDIIHQDSLNCDRRILAWVLAALMAAGNAVVRARLAARR
FLPPNVFPLSDITSAGGFSSLTGQELWIVSSVFPFLVSPIFEDAASLVRR
KLESSFTTRNGVGERHRVDERHGIECLDFVPFNAKGTMFIRCLARTPSPD
TTTSNPVQTRL*
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mRNA from alignment at L-elsbetiae_contig10653:3191..4814+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig10653.623.1 ID=mRNA_L-elsbetiae_contig10653.623.1|Name=mRNA_L-elsbetiae_contig10653.623.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1624bp|location=Sequence derived from alignment at L-elsbetiae_contig10653:3191..4814+ (Laminarionema elsbetiae ELsaHSoW15)
GATGGGATCCTGAGAGACCTTGCATACGTCTCGGCGGGCAACAGCAAGCC CGAGCGCACCGCACGGTCGAAGAGGAAGGGCGTCGTCGTCGGGGCTTTCC CCAATCCTCTAGCCATGGTCGTCTACGACCCAGTGCAACAGACAGGCATG GACATCATACATCAGGTATGTTGTCCATTTTGGGTTGTTGCTGCCAAGGA TAACCTCCGGTATTATTTTTGGTCCGTCTCGTCTTTCCTCTCATGGGCGT CTTTCCTATATATGGGAAGCTGAACCATTAATAAGGTGTGAGCGCGTTTG CCCCACCTTCCAGCCAGTTTTAGATACTGCAGCCGGACATAAATTTGGTT CTTTCTTAGCGCGGGGGCAGGCGCAGTAGCGCGACATGTTGCGGATTGAA ATATCGATGAACCCCCCCCCCACTGCTGTACCATCTTGCAACACTACCGG GGGCGATGGCGTGTTTGTCTCGATTTTTTACGTGTGTCTGTTTGGTTGCA CTTCCGGCTTGTGTATTGTCCAATGTGCTTTTCCGTCTTGCTGTATCATG TGTGTTGTACAACGGGTCGGGAGGACTAGATTGGTGGGCCTGACCGGCGA GAACCCGGCGCGGAACACCCCAGCATTGTTGGGGTACCAAGACAGAACCA GGGGGGGTTTCCCCCGCGGTTGGTTTTGTTGATTTTTCCAGTCTCGTTTT CACCTTACATAGCGTGCCGGGGGGGGGGGCGGAGAGCTCCACAAATTCTG TGTCTGAGACCTGTTGTTTTTGTGCTTTGCGGACCCTTCGGATCCATTTT GTTTTCAGTATGCCCTTTTGAGGAATCAACCGCGCGTCATTGTTGGATCA GTGAGGACTCATAATGGAGGTATGTATGTACTGGATAGAGAGATCGGAAC AGTTCTTACCGCAAGGCAACAACAATAACTTACGGCACGATGAAATTGCC TCTCCCTCTGGCGGGGCAGTTAGTTTTATCAACCCACCAGCGTGCTGACC TGACTTGACTCATTCGACTCGTTTGAAAGCGCCGCCCACGTATTTGGCTT TGCCACCCATCTTCTCTGCTATATGCTTCATAACCTGCAAAACACAGGAC AGCCTCAACTGCGACAGACGAATCCTAGCTTGGGTGCTGGCGGCTCTCAT GGCGGCGGGCAATGCCGTTGTGCGCGCGCGACTTGCGGCGCGTCGATTCC TACCGCCCAACGTTTTCCCGCTTTCAGACATAACAAGCGCGGGAGGCTTC TCGTCGCTTACGGGGCAAGAACTCTGGATCGTGTCAAGCGTGTTCCCGTT CCTTGTTTCGCCCATCTTTGAGGACGCCGCAAGCCTGGTGAGAAGAAAAC TCGAATCATCTTTTACCACAAGAAACGGAGTCGGCGAGAGACACAGAGTC GACGAAAGACACGGGATCGAGTGTCTTGATTTTGTCCCTTTCAATGCAAA AGGGACCATGTTTATACGCTGTCTCGCACGCACGCCCTCACCTGATACAA CAACATCAAATCCAGTTCAAACTCGACTCTAACAAAGCCCTAGGTCCGCT TGCGAGCCTCATAACATTTTTTGTCGCTCCCACCCTTCTGCGCGGGGGTA CTTCCTTTCCCCGTAATCCCCCCA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig10653:3191..4814+

>mRNA_L-elsbetiae_contig10653.623.1 ID=mRNA_L-elsbetiae_contig10653.623.1|Name=mRNA_L-elsbetiae_contig10653.623.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=972bp|location=Sequence derived from alignment at L-elsbetiae_contig10653:3191..4814+ (Laminarionema elsbetiae ELsaHSoW15)
ATGGTCGTCTACGACCCAGTGCAACAGACAGGCATGGACATCATACATCA
GATGGTCGTCTACGACCCAGTGCAACAGACAGGCATGGACATCATACATC
AGGACAGCCTCAACTGCGACAGACGAATCCTAGCTTGGGTGCTGGCGGCT
CTCATGGCGGCGGGCAATGCCGTTGTGCGCGCGCGACTTGCGGCGCGTCG
ATTCCTACCGCCCAACGTTTTCCCGCTTTCAGACATAACAAGCGCGGGAG
GCTTCTCGTCGCTTACGGGGCAAGAACTCTGGATCGTGTCAAGCGTGTTC
CCGTTCCTTGTTTCGCCCATCTTTGAGGACGCCGCAAGCCTGGTGAGAAG
AAAACTCGAATCATCTTTTACCACAAGAAACGGAGTCGGCGAGAGACACA
GAGTCGACGAAAGACACGGGATCGAGTGTCTTGATTTTGTCCCTTTCAAT
GCAAAAGGGACCATGTTTATACGCTGTCTCGCACGCACGCCCTCACCTGA
TACAACAACATCAAATCCAGTTCAAACTCGACTCTAAGACAGCCTCAACT
GCGACAGACGAATCCTAGCTTGGGTGCTGGCGGCTCTCATGGCGGCGGGC
AATGCCGTTGTGCGCGCGCGACTTGCGGCGCGTCGATTCCTACCGCCCAA
CGTTTTCCCGCTTTCAGACATAACAAGCGCGGGAGGCTTCTCGTCGCTTA
CGGGGCAAGAACTCTGGATCGTGTCAAGCGTGTTCCCGTTCCTTGTTTCG
CCCATCTTTGAGGACGCCGCAAGCCTGGTGAGAAGAAAACTCGAATCATC
TTTTACCACAAGAAACGGAGTCGGCGAGAGACACAGAGTCGACGAAAGAC
ACGGGATCGAGTGTCTTGATTTTGTCCCTTTCAATGCAAAAGGGACCATG
TTTATACGCTGTCTCGCACGCACGCCCTCACCTGATACAACAACATCAAA
TCCAGTTCAAACTCGACTCTAA
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