mRNA_L-elsbetiae_contig10039.84.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig10039.84.1
Unique NamemRNA_L-elsbetiae_contig10039.84.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: A0A6H5JH94_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JH94_9PHAE)

HSP 1 Score: 148 bits (373), Expect = 9.080e-40
Identity = 78/163 (47.85%), Postives = 105/163 (64.42%), Query Frame = 1
Query:    7 SPAVVTKTVTSDKFRLVFLAGLEGAGHHYVMAADSAMFSMNPDLPRISKDEELDVTPYYLPNVLGGDASRYAQAEDTARNEMRRLAEHAAGLPLPGSVYVLHNSWSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHRDFQDGLG 495
            S A++ KT  SD FRLVF+AGLEG GHHYVM A++++F  N +LPR       ++  YY P  +G +  ++  +   A   MRRLAE AA L  PG++       SYP   G  KVMQY+DL  IA+ AE +G+D RVLYLRRSA+++++ANTVHR F   +G
Sbjct:  135 STAILPKTHVSDVFRLVFVAGLEGTGHHYVMWANASLFGDNMNLPR-RYGHGKEIYEYYAPLKMGQNVKQFTMSSHNAVENMRRLAEWAANLSSPGALEFFSIPCSYPVNSGPLKVMQYMDLGMIAETAEGAGIDFRVLYLRRSAKEVVIANTVHRKFHLLMG 296          
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: D7G1E6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G1E6_ECTSI)

HSP 1 Score: 104 bits (259), Expect = 1.590e-25
Identity = 55/133 (41.35%), Postives = 85/133 (63.91%), Query Frame = 1
Query:  115 MFSMNPDLPRISKDEEL-DVTPYYLPNVLGGDASRYAQAEDTARNEMRRLAEHAAGLPLPGSVYVLHNSWSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHRDFQDGLGSSPSS 510
            MF  +PDL R   DE+L +   +++   +   ++ +  +    R++MR LA+ A  LP PG+V ++    SYP   G  KV QY+DL+ +A+ AE  GVD+RVLY++RSA+++L+A+TVHR FQ+ LG   SS
Sbjct:    1 MFKAHPDLVRF--DEKLPNFWKFFISTTMRTSSTAFTASWQQGRDQMRDLAQRAQTLPTPGTVQLITGGISYPCGNGPQKVQQYVDLRLLAEAAEAEGVDLRVLYMKRSAKELLLADTVHRGFQNFLGDIDSS 131          
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: D7FMV4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FMV4_ECTSI)

HSP 1 Score: 88.2 bits (217), Expect = 1.760e-17
Identity = 62/168 (36.90%), Postives = 80/168 (47.62%), Query Frame = 1
Query:   10 PAVVTKTVTSDKFRLVFLAGLEGAGHHYVMAADSAMFSMNPDLPRISKDEELDVTPYYLPNVLGGDASR--YAQAEDTARNEMRRLAEHAAGLPLPGSVYVLH---------NSWSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHRDF 480
            P VV K   S + R+VFL GLEG GHHY+  AD        D  R  K   L V    L   L    SR  Y  A    R EM +LA         G   V+             SYP + G  K +QY+D + +A+ AE +GVDMR +YL RSA DIL +++ H ++
Sbjct:   96 PVVVEKKAVSSRMRVVFLVGLEGTGHHYM--ADVLEDVCKKDKVRCPK---LCVMAKVLYPELSLPKSRNAYWDARKRLREEMEKLAAFPEESLAEGESTVVSFGACRFQGTGMMSYPNFNGDEKALQYVDFRLVAEEAERAGVDMRFVYLARSAADILTSDSGHHNY 258          
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: D7FMV3_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FMV3_ECTSI)

HSP 1 Score: 86.7 bits (213), Expect = 8.280e-17
Identity = 51/166 (30.72%), Postives = 80/166 (48.19%), Query Frame = 1
Query:   10 PAVVTKTVTSDKFRLVFLAGLEGAGHHYVMAADSAMFSMNPDLPRISKDEELDVTPYYLPNVLGGDASR-YAQAEDTARNEMRRLAEHAAGLPLPGSVYVLH--------NSWSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHRDF 480
            PAVV K   S + R++FL GLEG GHHY +     +F        +       +     P++      + Y +A    R E+  LA + A     G   +             S+P + G +K +QY+D + +A+ AE +GVD+R +YL RSA+ ILV++T H  +
Sbjct:   55 PAVVEKKTVSSQLRVMFLVGLEGTGHHYFLN----VFDDACTTAAVPCANACPIAKALYPSLATPSTKQDYERARRELRRELENLAAYPAEFMTEGQAAIAQFVTCRSKSGMMSFPNFNGNDKALQYVDFRLLAEEAERAGVDVRFVYLARSARSILVSDTQHNHY 216          
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: A0A7S0A0K6_9DINO (Hypothetical protein (Fragment) n=1 Tax=Pyrodinium bahamense TaxID=73915 RepID=A0A7S0A0K6_9DINO)

HSP 1 Score: 78.6 bits (192), Expect = 5.310e-14
Identity = 60/182 (32.97%), Postives = 88/182 (48.35%), Query Frame = 1
Query:   16 VVTKTVTSDKFRLVFLAGLEGAGHHYVM------------AAD-------------SAMFSMNPDL--PRISKDEELDVTPYYLPNVLGGDASRYAQAEDTARNEMRRLAEHAAGLPLPGSVYVLHNSWSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHRDF 480
            ++ K V   ++RL+F+AGLEG+GHH  +            AAD               ++S    L  PR +K  E     Y  P       S + +AE   R  +RRL+   A  PL  S+       SYP +PGK K+ Q  D++ +A++AE+ GVD R+L L R A  +L + T+HR F
Sbjct:   98 LLPKRVHGARWRLLFVAGLEGSGHHLWIPLLKRCVKARRCAADVELGRRLWTDRTRKGLWSFETKLYAPRAAKKWE-----YAPPGAKW--LSGFRRAEGEVRTALRRLSRRNATSPLLASLGGC-GMMSYPNFPGKLKMFQTPDVRLLAELAEDEGVDFRILVLHRPAAAVLRSTTIHRHF 271          
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: A0A7S3W283_9SPIT (Hypothetical protein (Fragment) n=1 Tax=Strombidinopsis acuminata TaxID=141414 RepID=A0A7S3W283_9SPIT)

HSP 1 Score: 70.5 bits (171), Expect = 1.290e-11
Identity = 52/168 (30.95%), Postives = 85/168 (50.60%), Query Frame = 1
Query:   19 VTKTVTSDKFRLVFLAGLEGAGHH--YVMAADSAMFSMNPDLPRISKDEELDVTPYYL---PNVLGGDASRYAQAEDTARNEMRRLAEHAAGLPLPGSVYVLHNS----WSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHRDFQDGLG 495
            V K   S+  R VF+AGLEG GHH  + M A S   S   +L   + D   D+    L    +++  + +    A+   +  + RL   A  +  P S+ + +N+     SYP   G +  M + DL+ +A++AE +GVD+RVL   R A D+L++  +H  F++  G
Sbjct:   63 VRKQQFSNTVRFVFVAGLEGTGHHLWHAMLAGSCARS---NLCANASDTLSDLIADKLHRKKSIMFSERASGRDAQLVEQKLVERLKIAARTVTSPTSLIIQNNALGGQMSYPDSVGTDVQMHHPDLRLLAELAEIAGVDLRVLLSMRGATDVLISTMIHHPFRNQKG 227          
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: A0A6H5KIA1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KIA1_9PHAE)

HSP 1 Score: 63.9 bits (154), Expect = 4.340e-9
Identity = 35/97 (36.08%), Postives = 50/97 (51.55%), Query Frame = 1
Query:  217 YAQAEDTARNEMRRLAEHAAGLPLPGSVYVLH---------NSWSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHRDF 480
            Y  A    R EM +LA         G   V+             SYP + G  K +QY+D + +A+ AE +GVDMR +YL RSA DIL +++VH ++
Sbjct:   39 YWDARKRLREEMEKLAAFPEESLAEGESTVVSFGACRFQGTGMMSYPNFNGDEKALQYVDFRLVAEEAERAGVDMRFVYLARSAADILTSDSVHHNY 135          
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: F0Y634_AURAN (PPM-type phosphatase domain-containing protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y634_AURAN)

HSP 1 Score: 64.3 bits (155), Expect = 7.610e-9
Identity = 58/183 (31.69%), Postives = 84/183 (45.90%), Query Frame = 1
Query:    1 TFSPAVVTKTVTSDKFRLVFLAGLEGAGHHYVMAADSAMFSMNPDLPRISK-DEELDVTPYYLPNVLGGDAS---------RYAQAEDTA-RNEMRRLAEHAAGLPLPGSVYVLH--------------NSWSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHR 474
            T  P  V KTV +   R +F+AGLEG+GHH +    S MF+M      + + D EL         +L G+ S         R A A+  A R       +H    P      VL+                 SYP +   +KV+ + D+Q +A MAE +G D+RVL + R A++ LV+ +VHR
Sbjct:   25 TTCPHYVKKTVDAPA-RFLFIAGLEGSGHHSL----SNMFAMCVRRRVLCRHDRELG-------QLLHGNRSHPTGIFVYGRQAAADIAALRKRFFARLQHLRDAPRDRGAPVLYLLNVNADGATIPDAGMMSYPNFGPPDKVLHHPDMQTLATMAEAAGADLRVLVVLRGARETLVSTSVHR 195          
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: A0A7S3K1J0_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3K1J0_9STRA)

HSP 1 Score: 58.2 bits (139), Expect = 8.820e-7
Identity = 42/175 (24.00%), Postives = 76/175 (43.43%), Query Frame = 1
Query:   25 KTVTSDKFRLVFLAGLEGAGHHYVMAADSAMFSMNPDL----------------------PRISKDEELDVTPYYLPNVLGGDASRYAQAEDTARNEMRRLAEHAA---GLPLPGSVYVLHNSWSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHR 474
            K + +DK R +F+AGLEG+GHH +     A  +    +                      P+ +   E++       ++L   A++++   +     +  +  H     G+   G +       SYP + GK K+  +  +Q IA  AE + VD+R+L L R ++ IL + T +R
Sbjct:  244 KRIFNDKIRFIFIAGLEGSGHHALREMFEACHTQGLCITSAQLMRALSNGREHPIGISVYPKDTNTTEINQRRNNFISILKQMATKFSDTSNDTLIILNTMIPHKTEMDGITHSGGI----GMQSYPNFNGKQKIYNHPSIQDIARAAENAKVDLRILVLARPSKAILRSTTKNR 414          
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Match: A0A7S2DFF0_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2DFF0_9STRA)

HSP 1 Score: 52.8 bits (125), Expect = 7.170e-6
Identity = 22/53 (41.51%), Postives = 38/53 (71.70%), Query Frame = 1
Query:  322 SYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDILVANTVHRDF 480
            SYP + G +K +   ++ ++A +AEE+GVD+R+L L+R A +++V+ T HR F
Sbjct:   33 SYPNFGGYDKALHRPNIHQLAMLAEEAGVDLRILVLQRPAHELMVSTTKHRKF 85          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig10039.84.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 11
Match NameE-valueIdentityDescription
A0A6H5JH94_9PHAE9.080e-4047.85Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G1E6_ECTSI1.590e-2541.35Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7FMV4_ECTSI1.760e-1736.90Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7FMV3_ECTSI8.280e-1730.72Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A7S0A0K6_9DINO5.310e-1432.97Hypothetical protein (Fragment) n=1 Tax=Pyrodinium... [more]
A0A7S3W283_9SPIT1.290e-1130.95Hypothetical protein (Fragment) n=1 Tax=Strombidin... [more]
A0A6H5KIA1_9PHAE4.340e-936.08Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
F0Y634_AURAN7.610e-931.69PPM-type phosphatase domain-containing protein n=1... [more]
A0A7S3K1J0_9STRA8.820e-724.00Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A7S2DFF0_9STRA7.170e-641.51Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig10039contigL-elsbetiae_contig10039:5585..6638 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score63.2
Seed ortholog evalue1.4e-07
Seed eggNOG ortholog44056.XP_009035729.1
KEGG koko:K10147,ko:K17506
KEGG Pathwayko04115,map04115
Hectar predicted targeting categoryother localisation
EggNOG free text desc.protein serine/threonine phosphatase activity
EggNOG OGsCOG0631@1,KOG0698@2759
EC3.1.3.16
COG Functional cat.T
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01009
Exons2
Model size518
Cds size516
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815237.1057324-CDS-L-elsbetiae_contig10039:5586..56171622815237.1057324-CDS-L-elsbetiae_contig10039:5586..5617Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig10039 5587..5617 -
1691682787.9603345-CDS-L-elsbetiae_contig10039:5586..56171691682787.9603345-CDS-L-elsbetiae_contig10039:5586..5617Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig10039 5587..5617 -
1622815237.1166172-CDS-L-elsbetiae_contig10039:6153..66381622815237.1166172-CDS-L-elsbetiae_contig10039:6153..6638Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig10039 6154..6638 -
1691682787.9725552-CDS-L-elsbetiae_contig10039:6153..66381691682787.9725552-CDS-L-elsbetiae_contig10039:6153..6638Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig10039 6154..6638 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig10039.84.1prot_L-elsbetiae_contig10039.84.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig10039 5587..6638 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig10039.84.1

>prot_L-elsbetiae_contig10039.84.1 ID=prot_L-elsbetiae_contig10039.84.1|Name=mRNA_L-elsbetiae_contig10039.84.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=172bp
TFSPAVVTKTVTSDKFRLVFLAGLEGAGHHYVMAADSAMFSMNPDLPRIS
KDEELDVTPYYLPNVLGGDASRYAQAEDTARNEMRRLAEHAAGLPLPGSV
YVLHNSWSYPTYPGKNKVMQYIDLQRIADMAEESGVDMRVLYLRRSAQDI
LVANTVHRDFQDGLGSSPSSNA
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mRNA from alignment at L-elsbetiae_contig10039:5585..6638-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig10039.84.1 ID=mRNA_L-elsbetiae_contig10039.84.1|Name=mRNA_L-elsbetiae_contig10039.84.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1054bp|location=Sequence derived from alignment at L-elsbetiae_contig10039:5585..6638- (Laminarionema elsbetiae ELsaHSoW15)
ACTTTTTCTCCTGCCGTAGTCACCAAAACGGTCACGAGCGACAAATTTCG CTTAGTTTTTCTCGCGGGTCTCGAGGGTGCTGGCCACCACTATGTCATGG CCGCTGACTCAGCCATGTTCAGCATGAACCCTGATCTTCCTCGAATCAGC AAGGACGAAGAGCTGGATGTAACGCCCTATTACTTGCCAAACGTTTTGGG CGGAGATGCCTCCCGCTACGCTCAAGCTGAAGACACGGCAAGGAACGAGA TGCGGAGGCTGGCGGAGCATGCGGCTGGCCTGCCTCTGCCGGGGAGTGTC TACGTCTTGCACAACTCTTGGTCCTACCCGACATACCCCGGAAAGAACAA GGTCATGCAATACATCGACCTGCAGAGAATAGCCGACATGGCGGAAGAAA GCGGGGTAGATATGCGCGTGCTGTACCTCCGACGATCCGCTCAGGACATC CTCGTCGCCAACACTGTTCACCGCGATTTTCAGGAGTGAGTGCAACTGCA TTTTCAGCATATCCGCCATGTTGCCATCAGTGTGCTGCTGGCTCGTTTTC AAAGCTGAAACAACCTCTTTCAATGCCGAGTCAAGACATTGACAGACTAT AGACTAAGTTTGTTTGTAATATCCAGATCGACCACAGCTCCGGGGCTTGA AACCGCGTCCAGTGCCCCGAATATAGGATTGAGTGTTCCCGGTTTATCCC CGAACCAATCATGCATCGCCTGGTCAAATATTGGTAGTATTATAGGTTAG ATAGGCGCTTCAACGTCAAAACTGGCCGTTCTTCGTCCAAATTTTGAGAA GATGATCCCTCAAGAGTTGCTGCGTAGAGTAGACGGCATCAAGAAACACT CCTCGTCCGGCAAAGCGTGCACCGCCAAGCTCGTATCCTGTCGCCATGCA CCCGCCAGCCTCCGAGCCCAACACTTTGAATGCGTACCAGGTTCGCACTT ATTCAGCAGAACTTTCCCCCTCCCTCCCCCCTCACGTAACGTGTTGCGCT TGTTTGCGTCGTAAAAAAAAGCGGACTTGGCAGCTCTCCTTCTTCAAACG CCCC
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Coding sequence (CDS) from alignment at L-elsbetiae_contig10039:5585..6638-

>mRNA_L-elsbetiae_contig10039.84.1 ID=mRNA_L-elsbetiae_contig10039.84.1|Name=mRNA_L-elsbetiae_contig10039.84.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1032bp|location=Sequence derived from alignment at L-elsbetiae_contig10039:5585..6638- (Laminarionema elsbetiae ELsaHSoW15)
ACTTTTTCTCCTGCCGTAGTCACCAAAACGGTCACGAGCGACAAATTTCG
CTTAGTTTTTCTCGCGGGTCTCGAGGGTGCTGGCCACCACTATGTCATGG
CCGCTGACTCAGCCATGTTCAGCATGAACCCTGATCTTCCTCGAATCAGC
AAGGACGAAGAGCTGGATGTAACGCCCTATTACTTGCCAAACGTTTTGGG
CGGAGATGCCTCCCGCTACGCTCAAGCTGAAGACACGGCAAGGAACGAGA
TGCGGAGGCTGGCGGAGCATGCGGCTGGCCTGCCTCTGCCGGGGAGTGTC
TACGTCTTGCACAACTCTTGGTCCTACCCGACATACCCCGGAAAGAACAA
GGTCATGCAATACATCGACCTGCAGAGAATAGCCGACATGGCGGAAGAAA
GCGGGGTAGATATGCGCGTGCTGTACCTCCGACGATCCGCTCAGGACATC
CTCGTCGCCAACACTGTTCACCGCGATTTTCAGGAACTTTTTCTCCTGCC
GTAGTCACCAAAACGGTCACGAGCGACAAATTTCGCTTAGTTTTTCTCGC
GGGTCTCGAGGGTGCTGGCCACCACTATGTCATGGCCGCTGACTCAGCCA
TGTTCAGCATGAACCCTGATCTTCCTCGAATCAGCAAGGACGAAGAGCTG
GATGTAACGCCCTATTACTTGCCAAACGTTTTGGGCGGAGATGCCTCCCG
CTACGCTCAAGCTGAAGACACGGCAAGGAACGAGATGCGGAGGCTGGCGG
AGCATGCGGCTGGCCTGCCTCTGCCGGGGAGTGTCTACGTCTTGCACAAC
TCTTGGTCCTACCCGACATACCCCGGAAAGAACAAGGTCATGCAATACAT
CGACCTGCAGAGAATAGCCGACATGGCGGAAGAAAGCGGGGTAGATATGC
GCGTGCTGTACCTCCGACGATCCGCTCAGGACATCCTCGTCGCCAACACT
GTTCACCGCGATTTTCAGGACGGACTTGGCAGCTCTCCTTCTTCAAACGC
CCGGACTTGGCAGCTCTCCTTCTTCAAACGCC
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