prot_L-digitata_M_contig9743.1.2 (polypeptide) Laminaria digitata LdigPH10_18mv male

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: D8LE29_ECTSI (Thiaminase II n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE29_ECTSI)

HSP 1 Score: 506 bits (1303), Expect = 1.780e-171
Identity = 313/609 (51.40%), Postives = 360/609 (59.11%), Query Frame = 0

Query:    1 MRSLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKVEDEGDVRAFHSLIGSVLDELGLHASYAAKWGLDIHNIPPPLKATSEYVNFLLGVASDPASSVAQVLASMAPCMRLYAFLGKELEHAFPLWGESVYAEWIETYSSEEFETAAVLVETLLDAHSAEAEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGG--SATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTADGQAEVDREWKVLTSLFLNNWSEVTEDSLKAKLAEE---DRSGGVDRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSFRTHVVSINWSRDLITGVLSDTLAGNDESSPAQLAARVDGGAGFSAASNSLLGEMKLPGFGGDWPAPVTSNDLKYDEHGVSTGDIAVKVSGSFGKHQHFLNLAKTARETAGGFEAVPRTMSVYVGDSVTDLMAMLDADVGIVVRNSDGGAFERVASAFGIAIRPLAAAYGALGEEGGNAGSEAEGEAGEPLEAGSTGRGSCVYRASGWAEIDVFLFG 604
            MRSLHHPWTLGLATG TS  SF++YVAQDAYFLRAFAKAYAYAL K EDE D+RAFHSLIGSVL+ELGLHASY+AKWG+D+ N                                             EL HAFP W ES YAEWIETYSSE+FE AA LVE+LLD +SA A+YPSL  LYRKAMELE+AFF AQ  G  E     +         SV   +GG    + Q G +   GR  FRGV  R+ALLCVDFDDTLT+GDT SLLV+TAK QR T + + ++ REW +LT  FL  WSE  EDSL  KL+     + SG VDR+GLE ML KL  VDLDSVSRVSDSK                                                   D+I+ VLS+TL  +  SS +          G  A      GE   P   GD PA V+SNDL +DEHGVSTG+I VK+ GSFGKHQ FL+LA TARE+    E+ PR M+VYVGDSVTDL+AMLDADVGIVV NS  G+FE+VA AFGI IRPLA+ Y A+           +G    P     +G G CVYRAS WAEIDVFLFG
Sbjct:   27 MRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN---------------------------------------------ELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQ---------SVGAEAGGRQGMSAQPGGQDAGGRGRFRGVHERLALLCVDFDDTLTEGDTISLLVETAKAQRETPEDRVDLGREWGLLTRTFLGKWSETIEDSLSTKLSTTCTTNGSGAVDREGLELMLRKLGVVDLDSVSRVSDSK---------------------------------------------------DVISRVLSETLLDSHSSSSSTT--------GIDAGGCVWAGETASPRLEGDCPASVSSNDLVFDEHGVSTGEILVKIPGSFGKHQRFLDLAATARESVASSESSPRLMTVYVGDSVTDLLAMLDADVGIVVGNS--GSFEKVARAFGIDIRPLASVYEAM-----------DGTGELPRTGAPSGSGQCVYRASQWAEIDVFLFG 509          

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: A0A6H5J5C1_9PHAE (TENA_THI-4 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J5C1_9PHAE)

HSP 1 Score: 474 bits (1220), Expect = 1.440e-157
Identity = 314/674 (46.59%), Postives = 371/674 (55.04%), Query Frame = 0

Query:    1 MRSLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKVEDEGDVRAFHSLIGSVLDELGLHASYAAKWGLDIHNIPP-------------PL------------------KATSEYVNFLLGV--ASDPASSVAQVLASMAPCMRLYA-------------------------------FLGKELEHAFPLWGESVYAEWIETYSSEEFETAAVLVETLLDAHSAEAEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGG--SATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTADGQAEVDREWKVLTSLFLNNWSEVTEDSLKAKLAEE----DRSGGVDRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSFRTHVVSINWSRDLITGVLSDTLAGNDESSPAQLAARVDGGAGFSAASNSLLGEMKLPGFGGDWPAPVTSNDLKYDEHGVSTGDIAVKVSGSFGKHQHFLNLAKTARETAGGFEAVPRTMSVYVGDSVTDLMAMLDADVGIVVRNSDGGAFERVASAFGIAIRPLAAAYGALGEEGGNAGSEAEGEAGEPLEAGSTGRGSCVYRASGWAEIDVFLFG 604
            MRSLHHPWTLGLATG TS  SF++YVAQDAYFLRAFAKAYAYAL K EDE D+RAFHSLIGSVL+ELGLHASY+AKWG+D+ N+               PL                  K  S++ + L  +   +      A+ L   A   R +A                               F+ +EL  A P W ES YAEWIETYSSE+FE AA LVE+LLD +SA A+YPSL  L RKAMELE+AFF AQ  G  E     +         SV   +GG    + Q G K   GR  FRGV  R+ALLCVDFDDTLT+GDTTSLLV+TAK QR T + + ++ REW +LT  FL+ WSE  EDSL  KL+      + SG VDR+GLE ML KL+ VDLDS SRVSDSKV                            R L++ +   F   ++                     +  P                     GE   P   GD PA V+SNDL +DEHGVSTG+IAVK+ GSFGKHQ FL+LA  ARE+    E+ PR M+VYVGDSVTDL+AMLDADVGIVV NS  G+FERVA AFGI I PLA+ Y A+           +G    P      G G  VYRAS W EIDVFLFG
Sbjct:   24 MRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANVRAKTTGVKLQIGSCTPLNFGLTGVWNVVHCRFASVKLHSDWAHLLYPINQGNKQMQVSAKELGERAMKRRSFAGLYLASLQIGLYRRQFHQCNVMTFADVLSSLFVAQELRDALPNWTESPYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALCRKAMELEFAFFDAQLDGVTEAAMLEQ---------SVGAEAGGRQGMSVQPGGKDAGGRGRFRGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSETIEDSLSTKLSTTACTTNGSGAVDREGLELMLRKLEVVDLDSASRVSDSKV----------------------------RELAREIRRYFLAAIIV--------------------QREP---------------------GETASPRLEGDCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFLDLAAMARESVASSESSPRLMTVYVGDSVTDLLAMLDADVGIVVGNS--GSFERVARAFGIDILPLASVYEAM-----------DGRGELPTTGAPLGSGQRVYRASQWTEIDVFLFG 606          

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: A0A0G4GYE5_VITBC (TENA_THI-4 domain-containing protein n=3 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4GYE5_VITBC)

HSP 1 Score: 246 bits (627), Expect = 9.290e-70
Identity = 200/625 (32.00%), Postives = 295/625 (47.20%), Query Frame = 0

Query:    1 MRSLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKVE--DEGDVRAFHSLIGSVLDELGLHASYAAKWGLDIHNIPPPLKATSEYVNFLLGVASDPASSVAQVLASMAPCMRLYAFLGKELEHAFPLWGESV--YAEWIETYSSEEFETAAVLVETLLDAHS-----------AEAEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGGSATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTADGQAEVDREWKVLTSLFLNNWSEVTEDSLKAKLAEEDRSGGVDRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSFRTHVVSINWSRDLITGVLSDTLAGNDESSPAQLAARVDGGAGFSAASNSLLGEMKLPGFGGDWPAPVTSNDLKYDEHGV-STGDIAVKVSGSFGKHQHFLNLAKTARETAGGFEAVPRTMSVYVGDSVTDLMAMLDADVGIVVRNS--DGGAFERVASAFGIAIRPL--AAAYGALGEEGGNAGSEAEGEAGEPLEAGSTGRGSCVYRASGWAEIDVFLFGD 605
            ++SL+HPW L L     +R S+  ++ QDA+FL  FAKAYA+AL K    D   +RAF++LIG VL EL LH S + +W +D     P  +AT  Y +FL  V +D  ++ A VLASMAPCM LYAF+GK L       G +   Y EW+ TYSS EF   A  VE LLD ++            +A   +LL  Y  AM+LEY FF AQ            +P P  + ++      G A               RGV   ++ + +DFD T T  DTT LL + A       D   +V + W+ +   F + +     + L+ K+    R G     GLEA L  L   +  +V+ VS++++L+G TR++V   +   LR  W+VR      LS  L+      ++S++WS+D+I  +L D                                       G     P+T N   + E    +TG+I+  ++G+  K + F  L    RE  G        +SV++GDSVTDL+A+L+AD+GIV++++       ++V S +GI ++PL  AA     G+E              P+          +Y A GW EI V LFG+
Sbjct:   62 LKSLYHPWVLRLGASVLARESYVEFMRQDAFFLEIFAKAYAHALCKCPPGDVEGIRAFNALIGGVLSELTLHQS-STEWAIDTSTFTPN-EATVRYTSFLRDVGADQWTTTAMVLASMAPCMSLYAFIGKALREGVGEEGMAGHRYNEWLATYSSAEFAQDAARVEELLDRYTDPLLSLSGQVGEDAVRGNLLYYYGNAMDLEYGFFDAQA-----------LPPPSCAPSASPSRHPGLAQ--------------RGV---LSFVAIDFDGTCTQRDTTPLLHEVAAEVHSDPD---QVRQRWRAIEDAFFDKYRRKVGELLEGKV----RDG---MNGLEAFLGGLGEFEKAAVADVSEARLLQGFTRDDVRRMVKTSLRQKWQVRDACGEVLSGLLSHDVPASIISVSWSQDMIHTILQDAC-------------------------------------GAPLSLPITCNSFSFREDDQRATGEISSHMTGAVDKLRAFRRL----REEKGR----EGRLSVFIGDSVTDLLALLEADIGIVLKDALNKNNTLDKVISLYGIDVQPLVRAAMIAQCGQEAATV---------TPVSMPPM----TIYAADGWDEIGVMLFGN 588          

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: A0A835Y9K0_9CHLO (Thiamine phosphate synthase n=1 Tax=Edaphochlamys debaryana TaxID=47281 RepID=A0A835Y9K0_9CHLO)

HSP 1 Score: 229 bits (584), Expect = 9.170e-61
Identity = 211/643 (32.81%), Postives = 271/643 (42.15%), Query Frame = 0

Query:    3 SLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKV--EDEGDVRAFHSLIGSVLDELGLHASYAAKWGLDIHNIPPPLKATSEYVNFLLGVASDPASSVAQVLASMAPCMRLYAFLGKELEHAFP--LWGESV-----------------------------YAEWIETYSSEEFETAAVLVETLLDAHSAEAEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGGSATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTADGQAEV--DREWKV-----LTSLFLNNWSEVTEDSLKAKLAEEDRSGGVDRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSFRTHVVSINWSRDLITGVLSDTLAGNDESSPAQLAARVDGGAGFSAASNSLLGEMKLPGFGGDWPAPVTSNDLKYDEHGVSTGDIAVKVSGSFGKHQHFLNL-AKTARETAGGFEAVPRTMSVYVGDSVTDLMAMLDADVGIVVRNSDGGAFERVASAFGIAIRPLAAAYGALGEEGGNAGSEAEGEAGEPLEAGSTGRGSCVYRASGWAEIDVFLFG 604
            SLH P+   +A G   RRSFQHYVAQDAYFL  FA+AYA AL K   ED+    A  +L+  V  EL LH +YA  WGL +    P   AT  Y +FL  VA DPA+SVA VLA+MAPC RLY FLG  L  A P  L G                                Y EW+ TY S E+     + E LLD  +A  +   L +LYR+AM+LE  F+AAQ         PHR P                                     R+  L VDFD+T T GDT   L++ A  Q   A G+  V  D  W       L   ++    E+    L  +  E       D  GL + L  L   D    + V    VL G T     EA          +RP     L+ AL       VVS+NWS  L+  VL    AG +   P + +AR               G ++L             N L+    G+++G +  +V  +  K      + AK+  +        P    VYVGDS +DL A+LDAD GIVV  +      RVA+A+GI +RPLAAA                     PL+  +   G+ +Y A GW EI  FLFG
Sbjct:   33 SLHEPFVQAMAQGTLDRRSFQHYVAQDAYFLHHFARAYALALAKAGAEDDATFEALSTLLRGVHAELKLHGAYAEGWGLALAKSQPS-PATKAYTDFLYEVAVDPATSVAAVLAAMAPCARLYGFLGCVLARAHPHNLPGAQAGQQQQEGGKEXXKGAEKGEGDEHAKAAAEYREWVRTYCSPEYLAVPDMKEELLDRLAAGEDEAHLQVLYRRAMQLEAEFWAAQ---------PHRPP-----------------------------------PRRLRALVVDFDETATPGDTIGALMRLA--QEAVAQGRPNVRGDAAWAASAPGRLAEGYVARQRELLASLLPPEGEEPSAPRPFDPSGLASFLTALSDFDESMNAVVEAEGVLAGST-----EAEQAAAGAQVALRPHCREALAGALRRGVPVWVVSVNWSHALVRAVLGLPCAGPEAEGPGEGSAR--------------SGAVEL-----------RCNSLELGPDGLTSGRLTKRVQSARDKQAQMQRIKAKSPTDNDQ-----PPAALVYVGDSASDLAALLDADYGIVVGTNR--LLRRVAAAYGIVLRPLAAA---------------------PLDPTTVPAGT-LYEADGWEEIRAFLFG 569          

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: A0A8J5XNG5_DIALT (Thiamine phosphate synthase n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XNG5_DIALT)

HSP 1 Score: 224 bits (572), Expect = 3.120e-59
Identity = 196/568 (34.51%), Postives = 268/568 (47.18%), Query Frame = 0

Query:    2 RSLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKVEDEGDVRAFHSLIGSVLDELGLHASYAAKWGLDIHNIPPPLKATSEYVNFLLGVASDPASSVAQVLASMAPCMRLYAFLGKELEHAFPLWGESVYAEWIETYSSEEFETAAVLVETLLDAHSAEAEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGGSATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTADGQAEVDREWKVLTSLFLNNWSEVTEDSLKAKLAEEDRSGGVDRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSF---RTHVVSINWSRDLITGVLSDTLAGNDESSPAQLAARVDGGAGFSAASNSLL----GEMKLPGFGGDWPAPVTSNDLKYDEHGVSTGDIAVKVSGSFGKHQHFLNL-AKTARETAGGFEAVPRTMSVYVGDSVTDLMAMLDADVGIVVRNSDGGAFERVASAFGIAIRPLAAAYGALGE 561
            RSLH P+ L LA G   R SFQ YVAQDA+FLRAFA+AYA AL++ E +G +    SLI  V+DEL LH  YAAKW LD+  + P   ATS Y  FLL +A   A  +A VLA+M PCMRLYAFLG+ L  AFP      YA+W+ TYSS+EF   A  +E LLD  +      +    Y +AM+LE  FFA+Q  G A  D    +  P D +                                  L  VDFD+T TD  T  LL + A    G AD +A    EW+ L+  F   ++   E  +           G     +  M+ +L   +  +V      + L+G+    + +A  G  R   K+R G  R L+ AL ++    R  V+S+NW+  L    L   L   D + P         GAG +  +  L+     EM +     D  AP T+ +L+           A  +  S  K   F  L A+ AR  AGG       +++Y GDS+TDL  +L A +GIV+ +S         S+ G+ ++PL AA  AL E
Sbjct:   19 RSLHSPFVLRLAAGTLPRASFQGYVAQDAFFLRAFARAYALALSRCESDGAIGELSSLIAGVVDELALHHGYAAKWDLDLAGVRPTA-ATSAYTEFLLALARSDAP-IAVVLAAMTPCMRLYAFLGQRLCAAFPD-ASHAYADWLRTYSSDEFGALAGRIEALLDECADGVPLDAARAAYTRAMQLELDFFASQP-GVAPVDG---LLAPADRL----------------------------------LFAVDFDETATDAQTLPLLAQLAAEHAGAADERAARVAEWRTLSETFAREYTMAIERIVPPXXXXXAPPAG--EAAVVEMVERLSAFERRAVEAAERGRALRGVPEAALAKA--GAERV--KLRDGFAR-LAYALRAAGACERAEVISVNWAAQLARAALRARL---DAARPV--------GAGATVGAEELVRLHCNEMPVAIDAHDG-APRTTGELR----------AAAGLHTSADKGAAFGQLCAEYARAHAGG---AGELLTLYAGDSITDLQPLLAARIGIVLGHSSS--LRAALSSLGVRVQPLVAARRALAE 511          

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: A0A2V0PBE0_9CHLO (Thiamine phosphate synthase n=1 Tax=Raphidocelis subcapitata TaxID=307507 RepID=A0A2V0PBE0_9CHLO)

HSP 1 Score: 219 bits (559), Expect = 1.730e-57
Identity = 182/568 (32.04%), Postives = 256/568 (45.07%), Query Frame = 0

Query:    2 RSLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKVED---EGDVRAFHSLIGSVLDELGLHASYAAKWGLDIHNIPPPLKATSEYVNFLLGVASDPASSVAQVLASMAPCMRLYAFLGKELEHAFPLWGESVYAEWIETYSSEEFETAAVLVETLLDAHSAEAEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGGSATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTADGQAE---VDREWKVLTSLFLNNWS--------EVTEDSLKAKLAEEDRSGGVDRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSFRTHVVSINWSRDLITGVLSDTLAGNDESSPAQLAARVDGGAGFSAASNSLLGEMKLPGFGGDWPAPVTSNDLKYDEHGVSTGDIAVKVSGSFGKHQHFLNLAKTARETAGGFEAVPRTMSVYVGDSVTDLMAMLDADVGIVVRNSDGGAFERVASAFGIAIRPLAAA 555
            R+L HP+ L L  G   R +F+HYVAQDA+FL  FA+AYA AL K  D   E  V     L+  V  EL LH SYA  WG+++  +     A   Y +FL+  A DP ++VA+VLASM PC RLY FLG  L  A P    + YA+WI  YSS E+    +  E LLD  + +A Y  L  LY++AM+LE AFF+A             IP                                      V +L  DFDDT T+ DT   L+  A        G  E   +      + +  + N+S        E+  +   A  AE    GG D  GL A + +L   D+   +   +S +L G++R++V  A          ++PG    L+ AL +   THVVS+NWSR+ +   L    A   +   A       G  G  AA+  + G    PG        + +N+L     G+STG +   V  +  K + F      A + A G        +V+VGDS TD++ +L AD GIVV ++      R  +  G ++ PL AA
Sbjct:   30 RALQHPFVLALGLGTLPRDTFRHYVAQDAHFLHFFARAYAAALDKCADANAEAHV-VLQQLLMGVHMELRLHGSYARTWGVELDALDHVDPACQAYTDFLMATAEDPETTVAEVLASMVPCSRLYGFLGCALARAHPRQRGNPYADWISMYSSFEYLVHPMQKEALLDRLAGDAPYDRLRSLYKRAMQLEVAFFSAAPG----------IP----------------------------------AARHVGMLVCDFDDTCTEKDTIGTLLSAAIDAAVKRAGPEEXXXLRARLDAVVARLVENYSSKHAALLAEILPEGASAAAAE----GGFDEAGLRAFVERLSDFDVQMNAVAVESGILAGLSRDDVAAAARAVA-----LKPGCREALAAALGAELPTHVVSVNWSREFVAAALGLPAADAADWGAA-------GSDGDEAAAAVIAGAAPPPG-----RLAIHANELARGADGLSTGALRRAVQCARDKGRLF-----AALQAARGKGGXXXXAAVFVGDSATDVLPLLAADYGIVVGSNK--LLRRALARHGASLVPLCAA 524          

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: A0A836B2K3_CHLIN (Thiamine phosphate synthase n=1 Tax=Chlamydomonas incerta TaxID=51695 RepID=A0A836B2K3_CHLIN)

HSP 1 Score: 219 bits (558), Expect = 2.190e-57
Identity = 203/625 (32.48%), Postives = 268/625 (42.88%), Query Frame = 0

Query:    3 SLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKVE--DEGDVRAFHSLIGSVLDELGLHASYAAKWGLDIHNIPP-PLKATSEYVNFLLGVASDPAS--SVAQVLASMAPCMRLYAFLGKELEHAF-PLWGESV-----YAEWIETYSSEEFETAAVLVETLLDAHSA-EAEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGGSATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTADGQAEVDREWKVLTSLFLNNWSEVTEDSLKAKLAEEDR--SGGVDRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSFRTHVVSINWSRDLITGVLSDTLAGNDESSPAQLAARVDGGAGFSAASNSLLGEMKLPGFGGDWPA-PVTSNDLKYDEHGVSTGDIAVKVSGSFGKHQHFLNL--------AKTARETAGGFEAVPRTMSVYVGDSVTDLMAMLDADVGIVVRNSDGGAFERVASAFGIAIRPLAAAYGALGEEGGNAGSEAEGEAGEPLEAGSTGRGSCVYRASGWAEIDVFLFG 604
            SLH P+  G+A G   +R+FQHYVAQDAYFL  FA+AY  AL+K    D+        L+  V +EL LH SYAAKWG+D+      P  AT  Y +FL+ VA       SVA++LA+M PC RLY FLG  L  A  P   +       Y EW+ TYSS ++ +   L E + +  +  EA+   LL LYR+AM+LE  FFAAQ         P+  P                                     R + L VDFD+T T  DT   L++ A    G        D  W   TS  L       +  L  +L  E    +   D  GL+A L  L   D    + V    +L+G T   V  A  G      ++RP     LS AL      HV+S+NW         SD   G   ++PA  A          +A       ++L G  G  PA  +  N L+ D  G++TG +A +V  +  K      L        A  A + A    +      VYVGDS +DL AMLD+D G+V+ ++      RV   FGI I PLAAA                     PL+      G+      GWAEI  FLFG
Sbjct:   30 SLHDPFVQGMAQGTLDKRAFQHYVAQDAYFLHYFARAYGIALSKALALDDSTFEDLSGLLRGVHEELQLHGSYAAKWGVDMTVAGTRPSAATKAYTDFLMNVADAEGQDGSVAEILAAMLPCARLYGFLGAALAGAHGPQAPDQAPPAHHYWEWVRTYSSADYLSVPGLKERMFERLAGKEADKAKLLRLYRRAMQLEAEFFAAQ---------PYSPP-----------------------------------PRRFSALVVDFDETCTAKDTIGGLMRLAAAAAGQQGRATAGDAAWVAATSSRLAADYVAAQGELLGRLLPEGAPDAAAYDAAGLDAFLAALSDFDAAMNAPVEACGILRGSTSAEVAAAGAG-----LQLRPQCREVLSGALGRGIPVHVLSVNW---------SDVYVGAALAAPATAAVXXXXXXXXXSAL-----PVRLAGAEGAGPAVELRCNSLELDGAGLTTGALAKRVQSARDKRAALAELRSRLPAAPAAAAVDGASDSGSDNPAALVYVGDSTSDLGAMLDSDFGVVIGSN--ALLRRVCRRFGIRILPLAAA---------------------PLDPRRVPAGTLYEAVGGWAEIRAFLFG 568          

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: A0A2K3DGU0_CHLRE (Thiamine phosphate synthase n=1 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A0A2K3DGU0_CHLRE)

HSP 1 Score: 217 bits (552), Expect = 1.430e-56
Identity = 202/631 (32.01%), Postives = 265/631 (42.00%), Query Frame = 0

Query:    3 SLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKVE--DEGDVRAFHSLIGSVLDELGLHASYAAKWGLDIHNIPP-PLKATSEYVNFLLGVASDPAS--SVAQVLASMAPCMRLYAFLGKELEHAF-PLWGESV-----YAEWIETYSSEEFETAAVLVETLLDAHSAE-AEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGGSATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTADGQAEVDREWKVLTSLFLNNWSEVTEDSLKAKLAEEDRSGGV--DRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSFRTHVVSINWSRDLITGVLSDTLAGNDESSPAQLAARVDGGAGFSAASNSLLGEMKLPGFGGDWPAPVT----SNDLKYDEHGVSTGDIAVKVSGSFGKHQHFLNL------AKTARETA-----GGFEAVPRTMSVYVGDSVTDLMAMLDADVGIVVRNSDGGAFERVASAFGIAIRPLAAAYGALGEEGGNAGSEAEGEAGEPLEAGSTGRGSCVYRASGWAEIDVFLFG 604
            SLH P+  G+A G   +R+FQHYVAQDAYFL  FA+AY  AL+K    DE        L+  V +EL LH SYAAKWG+D+      P  AT  Y +FL+ VA       SVA++LA+M PC RLY FLG  L  A  P   +       Y EW++TYSS+E+ +   L E + +  + + A+   LL LYR+AM+LE  FFAAQ         P+  P                                     R + L VDFD+T T  DT   L++ A    G        D  W   TS  L +     +  L  +L  E        D  GLEA+L  L   D      V +  +L+G T   V  A  G      ++RP     L+ AL      HV+S+NW         SD   G   S+ A                  L GE            P       N L+ D  G++TG +  +V  +  K      L      A TA   A      G  + P  + VYVGDS +DL AMLD+D G+V+ ++      RV   FG+ I PLAAA                     PL+      G+      GWAEI  FLFG
Sbjct:   30 SLHDPFVQGMAQGTLDKRAFQHYVAQDAYFLHYFARAYGIALSKALALDEQTFEDLTGLLRGVHEELQLHGSYAAKWGVDMTVAGTRPSAATKAYTDFLMDVADAGGQDGSVAEILAAMLPCARLYGFLGAALAGAHGPQAPDQAPPCHDYWEWVKTYSSQEYLSVPGLKERMFERLAGKGADKAKLLALYRRAMQLEAEFFAAQ---------PYSPP-----------------------------------PRRFSALVVDFDETCTARDTIGGLMRLAAAAAGQPGRATAGDAAWVSATSARLADAYVAAQGELLGRLLPEGAPDATCYDAAGLEALLAALSDFDAAMNGPVEECGILRGSTAAEVAAAGAG-----LQLRPQCREVLAGALGRGIPVHVLSVNW---------SDVYVGAALSAAAXXXXXXXXXXXXXXLPVRLAGEXXXXXXXXXXXGPAAVELLCNSLQLDGSGLTTGALTKRVQSARDKRAALAELRARLPAASTAAAAADGSTDSGSSSNPAAL-VYVGDSTSDLGAMLDSDFGVVIGSN--ALLRRVCRRFGVRILPLAAA---------------------PLDPRRVPAGTLYEAVGGWAEIRAFLFG 578          

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: A0A7S4PR83_GUITH (Hypothetical protein (Fragment) n=1 Tax=Guillardia theta TaxID=55529 RepID=A0A7S4PR83_GUITH)

HSP 1 Score: 205 bits (521), Expect = 1.800e-56
Identity = 147/415 (35.42%), Postives = 211/415 (50.84%), Query Frame = 0

Query:    2 RSLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKVEDEGD---VRAFHSLIGSVLDELGLHASYAAKWGLDIHNIPPPLKATSEYVNFLLGVASDPASSVAQVLASMAPCMRLYAFLGKELEHAFPLWG-----ESVYAEWIETYSSEEFETAAVLVETLLDAHSAE--AEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGGSATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTAKGQRGTADGQAEVDREWKVLTSLFLNNWSEVTEDSLKAKLAEEDRSGGVDRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSFRTHVVSINWSRDLITGVL 406
            R L+HP+  G+A G+  + SFQ+YVAQDA+FLR FA AY+ A   V D GD      FH+L   V +EL +H+SYA KW +D+ N+ P  K+T +YV+F++ +A   +  ++ + AS+ PCMRLYA+LG +L  A   +G     E++Y EWI TYSS+EFE  A  +E LLD ++ +   +Y  L+ +Y +AMELE+AFF AQ       D P                    A+  EG K D              +L VDFD TLT+ DTT  +VKT       A+ +    +    ++  F  N+ +  E  L       D     D + L      L   D  ++  V DS ++ GI ++ + E  TG   P  K      R L  +   +   HVVS+NWS+DLI   L
Sbjct:   22 RCLYHPFVSGIAQGSLKKSSFQNYVAQDAFFLRGFADAYSMARQIVSDNGDSPGAEKFHTLYNGVQEELRMHSSYAQKWNVDLTNVTPN-KSTQDYVDFVMEIAKKDSKKISLICASLTPCMRLYAWLGSKLGKA--RFGDVSSDENIYVEWINTYSSDEFEELAKTLEDLLDDYATKESTDYEDLVRIYSRAMELEFAFFDAQP------DLP--------------------ASSFEGIKPD--------------ILAVDFDQTLTEQDTTETIVKTGIHNIPDAEAREAQLQSMNNVSKTFYENYGKKIEGLLDENKPAAD---AYDPQALRRFFEGLAAFDKHALKPVEDSGLIAGIPQQGLIE--TGRNVPLQK---RAMRTLLHSARMNCHLHVVSVNWSKDLIRSAL 385          

BLAST of mRNA_L-digitata_M_contig9743.1.2 vs. uniprot
Match: A0A383WBI9_TETOB (Thiamine phosphate synthase n=2 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383WBI9_TETOB)

HSP 1 Score: 213 bits (541), Expect = 4.620e-55
Identity = 184/647 (28.44%), Postives = 269/647 (41.58%), Query Frame = 0

Query:    1 MRSLHHPWTLGLATGATSRRSFQHYVAQDAYFLRAFAKAYAYALTKVEDEGDVRAFHSLIGSVL----DELGLHASYAAKWGLDIHNIPPPLKATSEYVNFLLGVASDPASSVAQVLASMAPCMRLYAFLGKELEHAFPLWGESVYAEWIETYSSEEFETAAVLVETLLDAHSAEAEYPSLLILYRKAMELEYAFFAAQTQGTAEDDRPHRIPRPDDSMASVHISSGGSATFQEGEKRDEGREVFRGVQHRVALLCVDFDDTLTDGDTTSLLVKTA------------KGQRGTADGQAEVDREWKVLTSLFLNNWSEVTEDSLKAKLAEEDRSGGV---------DRKGLEAMLLKLQTVDLDSVSRVSDSKVLKGITRENVHEALTGPLRPSWKVRPGMTRCLSQALASSFRTHVVSINWSRDLITGVLSDTLAGNDESSPAQLAARVDGGAGFSAASNSLLGEMKLPGFGGDWPAPVTSNDLKYDEHGVSTGDIAVKVSGSFGKHQHFLNLAKTARETAGGFEAVPRTMS---VYVGDSVTDLMAMLDADVGIVVRNSDGGAFERVASAFGIAIRPLAAAYGALGEEGGNAGSEAEGEAGEPLEAGSTG-----------RGSCVYRASGWAEIDVFLFGDGHH 608
            M +LHHP+   LA G   R  FQHYV QD +FL  FA+AYA A+ K      + A  +++  +L     EL LH SYAAKWG+ +     P  ATS+Y +FL+ VA+DP +SVA+VLA+M PC RLY FLG +L  A        Y+EW+ TYS  E+  + V+ E L D  +A   Y  L  LY +AM+LE  FF+AQ  G A        PR                                     V LL  DFDDT+T+ DT   L+  A                     + +++ + K L   ++    ++    L A       + GV         D+ GL A    L   D++    V D+  L G+T+  V  A +     +  +RPG  R LS A A+     ++S+NWS+ L+  VL                                                + +N+L +   GVSTGD+  ++  +  K +    L +  R ++              VYVGDS +D++ +L AD GI++ ++      +  +AFG+ ++PL +A                     P+   S              G  +Y AS W EI  FLFG   H
Sbjct:   53 MEALHHPFVQALAAGTLDRACFQHYVLQDLFFLTRFARAYAAAIDKCGTTPALAAAKAVLAELLLGVDAELKLHGSYAAKWGVAVDEWVWPSAATSQYTDFLMQVATDPETSVAEVLAAMIPCARLYGFLGCQLAAATQGLPRHAYSEWVATYSGAEYLASPVMKEELFDKIAAAEPYDRLKQLYTRAMQLEVNFFSAQPNGPA--------PR------------------------------------HVGLLVCDFDDTITEKDTIGTLLGAAVEXXXXXXXXXXXXXXXXXXXRQQLNTQVKNLADNYMTKQQQLLASLLPADDDASAAAAGVSNSVTNKSYDKDGLLAFCEALSDFDVEMNQVVVDAGCLAGLTKAGVIAAAS-----NITLRPGSARTLSAAAAAGLPVALLSVNWSKSLMQAVLQPAAXXXX----------------XXXXXXXXXXXXXXXXXXXXXXMEIHANELVWGPDGVSTGDLQREIQCARDKGRVMDTLLQQHRSSSXXXXXXXXXXXXAVVYVGDSSSDILPLLKADYGIIIGSNK--LLRKALAAFGVGLQPLCSA---------------------PMTPSSXXXXXXXXXXXXXEGPVLYEASSWQEIAAFLFGPTWH 611