mRNA_L-digitata_M_contig99766.1.1 (mRNA) Laminaria digitata LdigPH10_18mv male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-digitata_M_contig99766.1.1
Unique NamemRNA_L-digitata_M_contig99766.1.1
TypemRNA
OrganismLaminaria digitata LdigPH10_18mv male (Laminaria digitata LdigPH10_18mv male (Oarweed))
Homology
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BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: A0A2E7PNM0_9PROT (1-deoxy-D-xylulose-5-phosphate synthase n=2 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A2E7PNM0_9PROT)

HSP 1 Score: 280 bits (715), Expect = 6.300e-88
Identity = 179/190 (94.21%), Postives = 183/190 (96.32%), Query Frame = 1 
Query:   10 DSSKTPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPAVGAMS 579
            ++SKTP+LDTVRIPADM DFTPEQLKQLAD+LR ETIDAVSITGGHLGAGLGVVELTVALHHVF AP DRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLA  DNNVVAVIGDGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX PAVGAMS
Sbjct:    3 NNSKTPILDTVRIPADMADFTPEQLKQLADDLRQETIDAVSITGGHLGAGLGVVELTVALHHVFDAPRDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAGNDNNVVAVIGDGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPAVGAMS 192
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BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: A0A2E8XNR6_9PROT (1-deoxy-D-xylulose-5-phosphate synthase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A2E8XNR6_9PROT)

HSP 1 Score: 268 bits (686), Expect = 1.290e-83
Identity = 135/151 (89.40%), Postives = 142/151 (94.04%), Query Frame = 1 
Query:   10 DSSKTPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGS 462
            + SKTPLLDTVRIPADM DF+PE+L+QLADELR ETIDAVSITGGHLGAGLGVVELTVALHHVF+ P DRLIWDVSHQ YPHKILT RRDRIRTLRQGGGLSGFTRR ESEYDPFGAAHSSTSISAGLGMAVARDL+E +NNVVAVIGDGS
Sbjct:    3 EDSKTPLLDTVRIPADMRDFSPERLRQLADELRQETIDAVSITGGHLGAGLGVVELTVALHHVFETPRDRLIWDVSHQSYPHKILTGRRDRIRTLRQGGGLSGFTRRAESEYDPFGAAHSSTSISAGLGMAVARDLSESENNVVAVIGDGS 153
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BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: A0A848WAC3_9PROT (1-deoxy-D-xylulose-5-phosphate synthase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A848WAC3_9PROT)

HSP 1 Score: 258 bits (659), Expect = 1.310e-79
Identity = 135/190 (71.05%), Postives = 148/190 (77.89%), Query Frame = 1 
Query:   10 DSSKTPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPAVGAMS 579
            D SKTPLLD+V+ PAD+ DF+ +QLKQ+ADELR ETIDAVSITGGHLGAGLGV+ELTVALHHVF+ P+DRLIWDVSHQ YPHKILT RRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDL+   NNV++VIGDG+                               APAVGAMS
Sbjct:    3 DQSKTPLLDSVKAPADLRDFSSDQLKQVADELRQETIDAVSITGGHLGAGLGVIELTVALHHVFETPKDRLIWDVSHQTYPHKILTGRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLSGGANNVISVIGDGAMSAGMAYEAMNNAGSMDSRLVVILNDNDMSIAPAVGAMS 192
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BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: A0A2U1VZQ0_9PROT (1-deoxy-D-xylulose-5-phosphate synthase (Fragment) n=2 Tax=Azospirillum sp. TSO22-1 TaxID=716789 RepID=A0A2U1VZQ0_9PROT)

HSP 1 Score: 239 bits (610), Expect = 1.370e-77
Identity = 125/188 (66.49%), Postives = 137/188 (72.87%), Query Frame = 1 
Query:   16 SKTPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPAVGAMS 579
            +KTPLLD VR+PAD+    P+ L+QLADELR+ETIDAVS+TGGHLGAGLGVVELTVALH+VF  P+DRLIWDV HQCYPHKILT RRDRIRTLR GGGLSGFT+R ES YDPFGAAHSSTSISAGLGMAV RDL  + N VV VIGDG+                               AP VGAMS
Sbjct:    5 AKTPLLDQVRVPADLRALRPDMLRQLADELRTETIDAVSVTGGHLGAGLGVVELTVALHYVFNTPDDRLIWDVGHQCYPHKILTGRRDRIRTLRMGGGLSGFTKRSESAYDPFGAAHSSTSISAGLGMAVGRDLKGRRNTVVCVIGDGAMSAGMAYEAMNNAGSMHSRLVVVLNDNDMSIAPPVGAMS 192
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BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: A0A2D4VHS3_9HYPH (1-deoxy-D-xylulose-5-phosphate synthase (Fragment) n=1 Tax=Parvibaculum sp. TaxID=2024848 RepID=A0A2D4VHS3_9HYPH)

HSP 1 Score: 240 bits (612), Expect = 2.890e-77
Identity = 127/186 (68.28%), Postives = 137/186 (73.66%), Query Frame = 1 
Query:   22 TPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPAVGAMS 579
            TPLLD V+ PAD+  F    L+QLADELR+ETIDAVS TGGHLGAGLGVVELTVALH+VF  P+DRLIWDV HQCYPHKILT RRDRIRTLRQGGGLSGFT+R ESEYDPFGAAHSSTSISAGLGMAVARDL+   NNV+AVIGDG+                               AP VGAMS
Sbjct:    7 TPLLDRVKTPADLRQFDESDLRQLADELRAETIDAVSQTGGHLGAGLGVVELTVALHYVFNTPDDRLIWDVGHQCYPHKILTGRRDRIRTLRQGGGLSGFTKRSESEYDPFGAAHSSTSISAGLGMAVARDLSGGKNNVIAVIGDGAMSAGMAYEAMNNAGSMDSRLIIILNDNDMSIAPPVGAMS 192
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BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: A0A3D3LKC2_9HYPH (1-deoxy-D-xylulose-5-phosphate synthase (Fragment) n=1 Tax=Rhodobiaceae bacterium TaxID=2026785 RepID=A0A3D3LKC2_9HYPH)

HSP 1 Score: 236 bits (602), Expect = 6.550e-77
Identity = 119/148 (80.41%), Postives = 130/148 (87.84%), Query Frame = 1 
Query:   19 KTPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGS 462
            +TPLLD +R+P D+ D + +QL QLADEL +ETI AVS TGGHLGAGLGVVELTVALH+VF  P+DRLIWDV HQ YPHKILT RRDRIRTLRQGGGLSGFT+R ES YDPFGAAHSSTSISAGLGMAVARDL E DNNVVAVIGDG+
Sbjct:    6 ETPLLDKIRLPEDVRDLSEDQLGQLADELCAETISAVSETGGHLGAGLGVVELTVALHYVFNTPDDRLIWDVGHQAYPHKILTGRRDRIRTLRQGGGLSGFTKRAESPYDPFGAAHSSTSISAGLGMAVARDLKETDNNVVAVIGDGA 153
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BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: A0A519QFF4_9CAUL (1-deoxy-D-xylulose-5-phosphate synthase (Fragment) n=1 Tax=Brevundimonas sp. TaxID=1871086 RepID=A0A519QFF4_9CAUL)

HSP 1 Score: 237 bits (605), Expect = 1.210e-76
Identity = 119/147 (80.95%), Postives = 128/147 (87.07%), Query Frame = 1 
Query:   22 TPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGS 462
            TPLLD V++PADM DF   QLKQLADE+R+ETIDAVS+TGGHLGAGLGVVELTVALHHVF+ P D LIWDV HQCYPHKILT RRDRIRTLR GGGLSGFT+R ESEYDPFGAAH+STSISA LG A ARD   +DN VVAVIGDGS
Sbjct:    4 TPLLDQVKVPADMRDFDIAQLKQLADEVRTETIDAVSVTGGHLGAGLGVVELTVALHHVFETPRDILIWDVGHQCYPHKILTGRRDRIRTLRMGGGLSGFTKRSESEYDPFGAAHASTSISAALGFAAARDQKGEDNRVVAVIGDGS 150
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BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: UPI000A581E68 (hypothetical protein n=1 Tax=Sneathiella glossodoripedis TaxID=418853 RepID=UPI000A581E68)

HSP 1 Score: 241 bits (616), Expect = 1.590e-76
Identity = 128/190 (67.37%), Postives = 139/190 (73.16%), Query Frame = 1 
Query:   10 DSSKTPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPAVGAMS 579
            ++ +TPLLD V+ P DM DFT +QL QLADELR ETI AVS+TGGHLGAGLGVVELTVALHHVF  PEDRLIWDV HQCYPHKILT RRDRIRTLRQ GGLSGFT+R ES YDPFGAAHSSTSISAGLGMAV RD+  ++NNVV+VIGDGS                               AP VGAMS
Sbjct:    3 ETFETPLLDQVKEPRDMRDFTLDQLIQLADELRQETISAVSVTGGHLGAGLGVVELTVALHHVFNTPEDRLIWDVGHQCYPHKILTGRRDRIRTLRQEGGLSGFTKRSESPYDPFGAAHSSTSISAGLGMAVGRDILGRENNVVSVIGDGSMSAGMAYEAMNNAGAMDSRQIVILNDNDMSIAPPVGAMS 192
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BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: A0A2E5KVK0_9PROT (1-deoxy-D-xylulose-5-phosphate synthase n=2 Tax=Proteobacteria TaxID=1224 RepID=A0A2E5KVK0_9PROT)

HSP 1 Score: 250 bits (638), Expect = 1.720e-76
Identity = 124/151 (82.12%), Postives = 136/151 (90.07%), Query Frame = 1 
Query:   10 DSSKTPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGS 462
            D SKTPLLDTV +PAD+ DFT +QL QLADELR+E +DAVS+TGGHLGAGLGVVELTVALHHVF  P+DRLIWDV HQ YPHKILT RRDRIRTLRQGGGL GFT+R ESE+DPFGAAHSSTSISAGLGMAVARDLA K NNV++VIGDG+
Sbjct:    3 DESKTPLLDTVTVPADIRDFTTDQLCQLADELRTELVDAVSVTGGHLGAGLGVVELTVALHHVFNTPDDRLIWDVGHQSYPHKILTGRRDRIRTLRQGGGLYGFTKRSESEFDPFGAAHSSTSISAGLGMAVARDLAGKSNNVISVIGDGA 153
[Close]
BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Match: A0A2N3E697_9PROT (1-deoxy-D-xylulose-5-phosphate synthase (Fragment) n=1 Tax=Alphaproteobacteria bacterium HGW-Alphaproteobacteria-11 TaxID=2013657 RepID=A0A2N3E697_9PROT)

HSP 1 Score: 234 bits (598), Expect = 3.600e-76
Identity = 118/147 (80.27%), Postives = 127/147 (86.39%), Query Frame = 1 
Query:   22 TPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHLGAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQGGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVIGDGS 462
            TPLLD V  PAD+  F    L+Q+ADELR+E IDAVS+TGGHLGAGLGVVELTVALHHVF  PEDR++WDV HQ YPHKILT RRDRIRTLRQGGGLSGFT+R ESEYDPFGAAHSSTSISAGLGMAVARDL    NNVVAVIGDG+
Sbjct:    7 TPLLDRVNSPADLRAFPESDLRQIADELRAEMIDAVSVTGGHLGAGLGVVELTVALHHVFNTPEDRIVWDVGHQAYPHKILTGRRDRIRTLRQGGGLSGFTKRDESEYDPFGAAHSSTSISAGLGMAVARDLKGGTNNVVAVIGDGA 153
The following BLAST results are available for this feature:
BLAST of mRNA_L-digitata_M_contig99766.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2E7PNM0_9PROT6.300e-8894.211-deoxy-D-xylulose-5-phosphate synthase n=2 Tax=Al... [more]
A0A2E8XNR6_9PROT1.290e-8389.401-deoxy-D-xylulose-5-phosphate synthase n=1 Tax=Al... [more]
A0A848WAC3_9PROT1.310e-7971.051-deoxy-D-xylulose-5-phosphate synthase n=1 Tax=Al... [more]
A0A2U1VZQ0_9PROT1.370e-7766.491-deoxy-D-xylulose-5-phosphate synthase (Fragment)... [more]
A0A2D4VHS3_9HYPH2.890e-7768.281-deoxy-D-xylulose-5-phosphate synthase (Fragment)... [more]
A0A3D3LKC2_9HYPH6.550e-7780.411-deoxy-D-xylulose-5-phosphate synthase (Fragment)... [more]
A0A519QFF4_9CAUL1.210e-7680.951-deoxy-D-xylulose-5-phosphate synthase (Fragment)... [more]
UPI000A581E681.590e-7667.37hypothetical protein n=1 Tax=Sneathiella glossodor... [more]
A0A2E5KVK0_9PROT1.720e-7682.121-deoxy-D-xylulose-5-phosphate synthase n=2 Tax=Pr... [more]
A0A2N3E697_9PROT3.600e-7680.271-deoxy-D-xylulose-5-phosphate synthase (Fragment)... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-digitata_M_contig99766contigL-digitata_M_contig99766:3..581 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Laminaria digitata LdigPH10_18mv male OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog1333998.M2A_2622
Preferred namedxs
PFAMsDXP_synthase_N,Transket_pyr,Transketolase_C
Model size579
Max annot lvl28211|Alphaproteobacteria
KEGG rclassRC00032
KEGG koko:K01662
KEGG ReactionR05636
KEGG Pathwayko00730,ko00900,ko01100,ko01110,ko01130,map00730,map00900,map01100,map01110,map01130
KEGG ModuleM00096
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.81e-75
EggNOG OGsCOG1154@1|root,COG1154@2|Bacteria,1MUSJ@1224|Proteobacteria,2TRDD@28211|Alphaproteobacteria,4BP5K@82117|unclassified Alphaproteobacteria
EC2.2.1.7
DescriptionCatalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP)
Cds size579
COG categoryH
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682408657.761735-CDS-L-digitata_M_contig99766:2..5811682408657.761735-CDS-L-digitata_M_contig99766:2..581Laminaria digitata LdigPH10_18mv maleCDSL-digitata_M_contig99766 3..581 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-digitata_M_contig99766.1.1prot_L-digitata_M_contig99766.1.1Laminaria digitata LdigPH10_18mv malepolypeptideL-digitata_M_contig99766 3..581 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-digitata_M_contig99766.1.1

>prot_L-digitata_M_contig99766.1.1 ID=prot_L-digitata_M_contig99766.1.1|Name=mRNA_L-digitata_M_contig99766.1.1|organism=Laminaria digitata LdigPH10_18mv male|type=polypeptide|length=193bp
SVIDSSKTPLLDTVRIPADMHDFTPEQLKQLADELRSETIDAVSITGGHL
GAGLGVVELTVALHHVFKAPEDRLIWDVSHQCYPHKILTDRRDRIRTLRQ
GGGLSGFTRRVESEYDPFGAAHSSTSISAGLGMAVARDLAEKDNNVVAVI
GDGSMSAGMAYEAMNNAGSMDTRLVVVLNDNDMSIAPAVGAMS
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mRNA from alignment at L-digitata_M_contig99766:3..581-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-digitata_M_contig99766.1.1 ID=mRNA_L-digitata_M_contig99766.1.1|Name=mRNA_L-digitata_M_contig99766.1.1|organism=Laminaria digitata LdigPH10_18mv male|type=mRNA|length=579bp|location=Sequence derived from alignment at L-digitata_M_contig99766:3..581- (Laminaria digitata LdigPH10_18mv male)
TCTGTGATCGATAGTAGCAAAACACCTCTTCTCGACACTGTCCGCATACC
GGCGGATATGCACGATTTCACACCCGAGCAGCTCAAGCAGCTCGCCGATG
AATTGCGCAGCGAGACCATTGATGCCGTATCGATCACCGGCGGCCATCTG
GGCGCGGGTCTGGGTGTCGTCGAGCTGACGGTTGCGCTTCATCATGTGTT
CAAGGCCCCCGAAGACCGCCTGATCTGGGATGTCAGCCATCAGTGCTATC
CCCACAAGATACTGACCGACCGACGTGATCGCATTCGCACCCTGCGTCAG
GGCGGCGGCCTGTCCGGTTTCACGCGACGGGTCGAAAGTGAATACGATCC
TTTTGGCGCGGCCCACAGCTCAACTTCGATCTCCGCTGGTCTCGGCATGG
CCGTGGCCCGCGATCTGGCAGAGAAAGACAATAATGTTGTCGCGGTGATC
GGTGATGGCTCCATGAGTGCGGGCATGGCCTATGAGGCGATGAACAACGC
GGGCTCAATGGATACCCGCCTGGTGGTTGTGCTCAACGACAATGATATGT
CGATCGCACCGGCGGTCGGGGCGATGAGC
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Coding sequence (CDS) from alignment at L-digitata_M_contig99766:3..581-

>mRNA_L-digitata_M_contig99766.1.1 ID=mRNA_L-digitata_M_contig99766.1.1|Name=mRNA_L-digitata_M_contig99766.1.1|organism=Laminaria digitata LdigPH10_18mv male|type=CDS|length=579bp|location=Sequence derived from alignment at L-digitata_M_contig99766:3..581- (Laminaria digitata LdigPH10_18mv male)
TCTGTGATCGATAGTAGCAAAACACCTCTTCTCGACACTGTCCGCATACC
GGCGGATATGCACGATTTCACACCCGAGCAGCTCAAGCAGCTCGCCGATG
AATTGCGCAGCGAGACCATTGATGCCGTATCGATCACCGGCGGCCATCTG
GGCGCGGGTCTGGGTGTCGTCGAGCTGACGGTTGCGCTTCATCATGTGTT
CAAGGCCCCCGAAGACCGCCTGATCTGGGATGTCAGCCATCAGTGCTATC
CCCACAAGATACTGACCGACCGACGTGATCGCATTCGCACCCTGCGTCAG
GGCGGCGGCCTGTCCGGTTTCACGCGACGGGTCGAAAGTGAATACGATCC
TTTTGGCGCGGCCCACAGCTCAACTTCGATCTCCGCTGGTCTCGGCATGG
CCGTGGCCCGCGATCTGGCAGAGAAAGACAATAATGTTGTCGCGGTGATC
GGTGATGGCTCCATGAGTGCGGGCATGGCCTATGAGGCGATGAACAACGC
GGGCTCAATGGATACCCGCCTGGTGGTTGTGCTCAACGACAATGATATGT
CGATCGCACCGGCGGTCGGGGCGATGAGC
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