mRNA_L-digitata_M_contig101415.1.1 (mRNA) Laminaria digitata LdigPH10_18mv male

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: A0A2D7FKI7_9PROT (Pyruvate kinase n=1 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A2D7FKI7_9PROT)

HSP 1 Score: 518 bits (1333), Expect = 1.900e-181
Identity = 272/308 (88.31%), Postives = 289/308 (93.83%), Query Frame = 1

Query:    1 TMADGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            T+ADGG++LE+GQAFRLDLND  G   RAPLPHPEIFAALKEDTELL+DDGKIRLRVTEAG GFAET+V AGG+LSDRKGVN+PKAVLPLAAMTDKDR DLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPA IDRLDEII LSDA+MVARGDLGVELPPEDVPSLQK+I+R CREAGKPVVVATQMLESM+SA APTRAEASDVATAIYDGADAVML AETA+G+YP +AVAIMDRIIARVERD+AYR IM+TSHPDPESTAPDAITAAARQVAETI A VIVTYTT GST LRA
Sbjct:   79 TIADGGIMLERGQAFRLDLNDLKGSKDRAPLPHPEIFAALKEDTELLVDDGKIRLRVTEAGAGFAETLVEAGGQLSDRKGVNLPKAVLPLAAMTDKDRVDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAAIDRLDEIIALSDAIMVARGDLGVELPPEDVPSLQKQIVRRCREAGKPVVVATQMLESMVSAQAPTRAEASDVATAIYDGADAVMLSAETAAGEYPVQAVAIMDRIIARVERDEAYRAIMDTSHPDPESTAPDAITAAARQVAETINATVIVTYTTSGSTTLRA 386          

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: A0A520NUU9_9PROT (Pyruvate kinase n=1 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A520NUU9_9PROT)

HSP 1 Score: 488 bits (1257), Expect = 6.720e-170
Identity = 254/307 (82.74%), Postives = 279/307 (90.88%), Query Frame = 1

Query:    4 MADGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            M  GG+ L++ QAFRLDL + TGD  RAPLPHPEIF ALKE+T++LL+DG IRLRVTE GDGFA+TVV AGG L+DRKGVNVP AVLP+AAMTDKDR DL+FAL++GVDW+ALSFVQR EDVAEARKI+QGRAAVLSKLEKPA IDRL+EIITLSDAVMVARGDLGVELPPEDVP +QK+IIR CREAGKPVVVATQMLESMISAPAPTRAEASDVATA+YDGADAVML AETA+G YP EAV+IMDRIIAR ERD+AYR IM+T+HPDPE TAPDAITAAARQVAETIGAA IVTYTT GST LRA
Sbjct:   80 MEKGGIDLQKDQAFRLDLEELTGDRDRAPLPHPEIFTALKENTQILLNDGNIRLRVTEVGDGFADTVVTAGGILTDRKGVNVPNAVLPVAAMTDKDRADLEFALTMGVDWIALSFVQRAEDVAEARKIVQGRAAVLSKLEKPAAIDRLEEIITLSDAVMVARGDLGVELPPEDVPGIQKQIIRQCREAGKPVVVATQMLESMISAPAPTRAEASDVATAVYDGADAVMLSAETAAGDYPVEAVSIMDRIIARTERDNAYRAIMDTTHPDPEQTAPDAITAAARQVAETIGAAAIVTYTTSGSTTLRA 386          

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: A0A2D8PRY1_9PROT (Pyruvate kinase (Fragment) n=1 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A2D8PRY1_9PROT)

HSP 1 Score: 478 bits (1230), Expect = 4.900e-166
Identity = 248/307 (80.78%), Postives = 274/307 (89.25%), Query Frame = 1

Query:    4 MADGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            +A  G  L +G + RLDL+D  GD SRAPLPHPEIF+AL+E T++LL+DGKIRL+VTE GDGFA+T V AGG L+DRKGVN+P AVLPLAAMT KDR DLDFALSIG+DWVALSFVQRPEDVAEARKI+QGRAAVLSKLEKPA ID+LDEII LSDA+MVARGDLGVE+PPEDVP +QK+IIR CREAGKPVVVATQMLESMIS PAPTRAEASDVATA+YDGADAVML AETA+G YP EAVAIMDRIIAR ERD+AY  IM T+HPDPE+TAPDAITAAARQVA+TIGAA IVTYTT GST LRA
Sbjct:   64 IAGDGAELAEGSSLRLDLDDAVGDESRAPLPHPEIFSALREGTQILLNDGKIRLKVTEVGDGFAQTTVTAGGLLTDRKGVNIPDAVLPLAAMTGKDRDDLDFALSIGIDWVALSFVQRPEDVAEARKIVQGRAAVLSKLEKPAAIDQLDEIIILSDAIMVARGDLGVEMPPEDVPGIQKQIIRKCREAGKPVVVATQMLESMISDPAPTRAEASDVATAVYDGADAVMLSAETAAGNYPVEAVAIMDRIIARTERDEAYSAIMNTAHPDPENTAPDAITAAARQVADTIGAAAIVTYTTSGSTTLRA 370          

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: A0A2S6Q933_9PROT (Pyruvate kinase n=1 Tax=Alphaproteobacteria bacterium MarineAlpha11_Bin1 TaxID=2013106 RepID=A0A2S6Q933_9PROT)

HSP 1 Score: 447 bits (1149), Expect = 1.670e-153
Identity = 227/305 (74.43%), Postives = 266/305 (87.21%), Query Frame = 1

Query:   10 DGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            +GG+ LE+GQAF+LD++D  GD  RAPLPHPEIF AL ++T++L+DDGKI L+V +  DG A TVV  GGRL+DRKG+N+P AVLP+ A+TDKDR DL  AL + VDWVALSFVQRPED+ EAR I++GRAA+LSKLEKP+ ID+LDEII+LSDAVMVARGDLGVELPPED+P LQK+II  CR+AGKPVVVATQMLESMI +P+PTRAEASDVATA+YDGADAVML AETA+G YP EAV+IMDRI+AR ERD+AY  +M+T+HPDPESTAPDAITAAARQVAETI AA IVTYTT GST LRA
Sbjct:   82 NGGIDLEEGQAFQLDMDDKIGDQDRAPLPHPEIFTALTKNTQILIDDGKILLQVVDIKDGLANTVVKVGGRLTDRKGLNIPNAVLPVPAITDKDRVDLQLALKLEVDWVALSFVQRPEDLVEARDIVKGRAALLSKLEKPSAIDKLDEIISLSDAVMVARGDLGVELPPEDIPGLQKQIIHRCRDAGKPVVVATQMLESMIDSPSPTRAEASDVATAVYDGADAVMLSAETAAGDYPIEAVSIMDRIVARTERDNAYHAVMKTTHPDPESTAPDAITAAARQVAETIDAAAIVTYTTSGSTTLRA 386          

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: A0A2D8G319_9PROT (Pyruvate kinase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A2D8G319_9PROT)

HSP 1 Score: 444 bits (1143), Expect = 1.500e-152
Identity = 232/305 (76.07%), Postives = 264/305 (86.56%), Query Frame = 1

Query:   10 DGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            +GGV L +GQ FRLDL+   GD  R PL HPEIF+AL+ DT++ LDDGKIRL+V +AG  +AETVV AGG L+DRKGVNVP AVLP+AAMTDKDR+DL+ AL IGVDW+ALSFVQRP D+AEARK+I  RAA++SKLEKP+ I+RL+EII LSDA+MVARGDLGVELPPEDVP+LQK+IIR CRE GKPVVVATQMLESMI+AP PTRAEASD+ATAIYDGADAVML AETA G+YP EAV+IM+RII R E+DDAYR IM T+HPDPE TA DAITAAARQVAETIGA  IVTYTT GST LRA
Sbjct:   82 EGGVHLGEGQTFRLDLDKALGDHDRVPLLHPEIFSALRTDTQIFLDDGKIRLQVVDAGKDYAETVVTAGGLLTDRKGVNVPNAVLPVAAMTDKDRSDLEHALLIGVDWIALSFVQRPNDLAEARKLICDRAAIISKLEKPSAIERLEEIIELSDAIMVARGDLGVELPPEDVPALQKQIIRQCRETGKPVVVATQMLESMINAPTPTRAEASDIATAIYDGADAVMLSAETAVGEYPVEAVSIMNRIINRTEQDDAYRAIMVTTHPDPERTAQDAITAAARQVAETIGATAIVTYTTSGSTTLRA 386          

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: A0A6N7FD60_9PROT (Pyruvate kinase n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A6N7FD60_9PROT)

HSP 1 Score: 429 bits (1103), Expect = 1.590e-146
Identity = 227/305 (74.43%), Postives = 259/305 (84.92%), Query Frame = 1

Query:   10 DGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            DG V L  G+AFRLDL+ T GD+ RAPLPHPEIFAA++E TE+LLDDGK+RLRVT A   FAET V+ GGRLS+ KGVNVP A LP++A+  KDR DL FA+  GVD++ LSFVQRPEDVAEARK++ GRAA+LSKLEKP+ I  LDEII LSDAVMVARGDLGVELPPEDVPSLQK+I+R+CR+ GKPVVVATQMLESMI APAPTRAEASDVATA+YDGADAVML AETA+G+YP EAV +M+RIIARVE+D  YR I +  H DPE+TAPDAITAAARQVA T+ AA IVTYTT GST LRA
Sbjct:   82 DGAVELVAGRAFRLDLDRTPGDSGRAPLPHPEIFAAIEEGTEVLLDDGKLRLRVTRADRKFAETEVMVGGRLSNHKGVNVPNATLPISALGAKDRDDLRFAVDHGVDYIGLSFVQRPEDVAEARKLVAGRAAILSKLEKPSAIQHLDEIIMLSDAVMVARGDLGVELPPEDVPSLQKQIVRACRQHGKPVVVATQMLESMIGAPAPTRAEASDVATAVYDGADAVMLSAETAAGKYPREAVEMMERIIARVEQDPQYRVIRDAEHHDPEATAPDAITAAARQVARTVSAAAIVTYTTSGSTSLRA 386          

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: UPI000D657450 (pyruvate kinase n=1 Tax=Azospirillum sp. TSO22-1 TaxID=716789 RepID=UPI000D657450)

HSP 1 Score: 427 bits (1097), Expect = 3.570e-145
Identity = 229/308 (74.35%), Postives = 253/308 (82.14%), Query Frame = 1

Query:    1 TMADGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            T A G V L  GQ+FRLDL+   GD +R  +PHPEIFAAL+ + ELLLDDGK+RLRVT  G  FAETVV+AG RLSDRKGVNVP  VLPL+ +T KDRTDL FAL  GVDWVALSFVQRPEDVAEARK+I GRAA+LSKLEKP  I  L+ I+ LSD VMVARGDLGVE+PPEDVPS+QKRI+R  R AGKPV+VATQMLESMISAPAPTRAEASDVATA++DGADAVML AETASG YP EAV+IMDRI  RVE+D  YR +M+  H DPE TA DAITAAARQVA TI AA IVTYTT GST LRA
Sbjct:   85 TFAGGPVDLTPGQSFRLDLSREPGDAARVGMPHPEIFAALEPEAELLLDDGKVRLRVTACGADFAETVVIAGTRLSDRKGVNVPGVVLPLSPLTAKDRTDLAFALDQGVDWVALSFVQRPEDVAEARKLIAGRAALLSKLEKPQAIQHLERIVELSDGVMVARGDLGVEMPPEDVPSIQKRIVREARMAGKPVIVATQMLESMISAPAPTRAEASDVATAVFDGADAVMLSAETASGAYPIEAVSIMDRIARRVEQDTLYRAMMDAQHADPEQTAADAITAAARQVAHTIKAAAIVTYTTSGSTTLRA 392          

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: UPI001AE3E970 (pyruvate kinase n=1 Tax=Azospirillum picis TaxID=488438 RepID=UPI001AE3E970)

HSP 1 Score: 426 bits (1096), Expect = 4.440e-145
Identity = 228/306 (74.51%), Postives = 249/306 (81.37%), Query Frame = 1

Query:    7 ADGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            ADG V L  GQ FRLDL+D  G+  R  +PHPEIFAAL  D ELLLDDGK+RLRVT  G  +AETVVV+G RLSDRKGVNVP  VLPL+ +T+KDR DL FAL  GVDWVALSFVQRPEDVAEAR++I GRAA+LSKLEKP  I  LD I+ LSD VMVARGDLGVE+PPEDVPSLQKRIIRS R AGKPV+VATQMLESMI APAPTRAEASDVATA++DG DAVML AETASG+YP EAV+IMDRI  RVE D  YR +M+  H DPE TA DAITAAARQVA TI AA I TYTT GST LRA
Sbjct:   87 ADGPVALAPGQLFRLDLSDEPGNARRVGMPHPEIFAALVPDAELLLDDGKVRLRVTACGPDYAETVVVSGTRLSDRKGVNVPGVVLPLSPLTEKDRRDLAFALDQGVDWVALSFVQRPEDVAEARRLIAGRAALLSKLEKPQAIQHLDHIVELSDGVMVARGDLGVEMPPEDVPSLQKRIIRSARAAGKPVIVATQMLESMIGAPAPTRAEASDVATAVFDGTDAVMLSAETASGEYPVEAVSIMDRIARRVENDPLYRAMMDAQHADPEQTASDAITAAARQVAHTISAAAIATYTTSGSTTLRA 392          

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: UPI001FEB4155 (pyruvate kinase n=1 Tax=Azospirillum soli TaxID=1304799 RepID=UPI001FEB4155)

HSP 1 Score: 426 bits (1094), Expect = 1.500e-144
Identity = 227/306 (74.18%), Postives = 252/306 (82.35%), Query Frame = 1

Query:    7 ADGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            A+G V L+ GQ FRLDL+   GD SR  +PHPEIFAAL  +T+LLLDDGK+RLRV + G  FAETVVV+G RLSDRKGVNVP  VLPL+ +T KDR DL FAL  GVDWVALSFVQRPEDVAEARK+I GRAA+LSK+EKP  I  L+ I+ LSD VMVARGDLGVELPPEDVP++QKRI+R  R AGKPV+VATQMLESMISAPAPTRAEASDVATAI+DGADAVML AETASG YP EAV+IMDRI  RVE D  YR +M+  HPDP+ TA DAITAAARQVA TI AA IVTYTT GST LRA
Sbjct:   87 ANGPVELKAGQRFRLDLSTEPGDESRVGMPHPEIFAALMPETDLLLDDGKVRLRVVDCGPDFAETVVVSGTRLSDRKGVNVPDVVLPLSPLTAKDRADLAFALDQGVDWVALSFVQRPEDVAEARKLIDGRAALLSKMEKPQAIQHLERIVELSDGVMVARGDLGVELPPEDVPTIQKRIVREARRAGKPVIVATQMLESMISAPAPTRAEASDVATAIFDGADAVMLSAETASGAYPIEAVSIMDRIARRVEHDPLYRTMMDAQHPDPQETAADAITAAARQVAHTIHAAAIVTYTTSGSTTLRA 392          

BLAST of mRNA_L-digitata_M_contig101415.1.1 vs. uniprot
Match: A0A3N1KV25_9PROT (Pyruvate kinase n=1 Tax=Stella humosa TaxID=94 RepID=A0A3N1KV25_9PROT)

HSP 1 Score: 423 bits (1087), Expect = 4.100e-144
Identity = 220/308 (71.43%), Postives = 256/308 (83.12%), Query Frame = 1

Query:    1 TMADGGVLLEQGQAFRLDLNDTTGDTSRAPLPHPEIFAALKEDTELLLDDGKIRLRVTEAGDGFAETVVVAGGRLSDRKGVNVPKAVLPLAAMTDKDRTDLDFALSIGVDWVALSFVQRPEDVAEARKIIQGRAAVLSKLEKPAGIDRLDEIITLSDAVMVARGDLGVELPPEDVPSLQKRIIRSCREAGKPVVVATQMLESMISAPAPTRAEASDVATAIYDGADAVML*AETASGQYPFEAVAIMDRIIARVERDDAYRGIMETSHPDPESTAPDAITAAARQVAETIGAAVIVTYTTPGSTPLRA 924
            T ADG ++L+ GQ FRLDL D  GD +RAPLPHPEIF A+   T+LLLDDG+IRLRV +     AET+V+ GG LS+RKGVNVP AVLP++A+TDKDR DL F L IG DW+ALSFVQRPEDVAEARK+I+GRAAV+ KLEKP  I+RL EI+ L+DAVMVARGDLGVE+PPEDVP LQKRI+R+CR AGKPVVVATQMLESM+ +PAPTRAEASDVATA+YDGADAVML AETA+G YP EAV IM+RIIA+VE+D  YR IM+  H  PE TA DAI+AAA QV++TIGAA I T+TT GST LRA
Sbjct:   79 TFADGKIMLKAGQGFRLDLTDKPGDETRAPLPHPEIFEAISPGTDLLLDDGRIRLRVEKVRGKVAETLVITGGPLSNRKGVNVPNAVLPVSALTDKDRRDLRFGLDIGADWIALSFVQRPEDVAEARKLIEGRAAVMVKLEKPQAIERLQEILDLTDAVMVARGDLGVEMPPEDVPGLQKRIVRACRVAGKPVVVATQMLESMVQSPAPTRAEASDVATAVYDGADAVMLSAETAAGAYPREAVEIMNRIIAKVEQDPLYRPIMDAEHSQPEHTAADAISAAAAQVSQTIGAAAICTFTTSGSTTLRA 386