mRNA_L-digitata_M_contig1007.2.1 (mRNA) Laminaria digitata LdigPH10_18mv male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-digitata_M_contig1007.2.1
Unique NamemRNA_L-digitata_M_contig1007.2.1
TypemRNA
OrganismLaminaria digitata LdigPH10_18mv male (Laminaria digitata LdigPH10_18mv male (Oarweed))
Homology
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BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: D8LCE1_ECTSI (Glutamate synthase (NADH/NADPH-dependent), N-terminal part n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCE1_ECTSI)

HSP 1 Score: 95.1 bits (235), Expect = 4.500e-22
Identity = 50/57 (87.72%), Postives = 53/57 (92.98%), Query Frame = 1 
Query:    1 DAAARAARMPNRK---PLAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQV 162
            D AARAAR+PNR+   PLAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQ+
Sbjct:   44 DEAARAARLPNRRGGRPLAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQI 100
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BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: A0A6H5KZS8_9PHAE (Glutamine amidotransferase type-2 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KZS8_9PHAE)

HSP 1 Score: 93.2 bits (230), Expect = 1.170e-20
Identity = 49/57 (85.96%), Postives = 52/57 (91.23%), Query Frame = 1 
Query:    1 DAAARAARMPNRK---PLAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQV 162
            D AARAAR+PNR+   PLAGPRTLP KRVGLYDPALEKDSCGVGLAVQMKSVASRQ+
Sbjct:   37 DEAARAARLPNRRGGRPLAGPRTLPAKRVGLYDPALEKDSCGVGLAVQMKSVASRQI 93
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BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: A0A835YLR4_9STRA (Glutamine amidotransferase type-2 domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YLR4_9STRA)

HSP 1 Score: 58.9 bits (141), Expect = 1.350e-8
Identity = 28/42 (66.67%), Postives = 32/42 (76.19%), Query Frame = 1 
Query:   37 KPLAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQV 162
            KP +  R  P  R+GLYDPALEKDSCGVGL VQ+KS ASR +
Sbjct:   31 KPSSAVRVFPKGRMGLYDPALEKDSCGVGLCVQLKSKASRAI 72
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BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: A0A2E4UQE6_9GAMM (Glutamate synthase large subunit n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A2E4UQE6_9GAMM)

HSP 1 Score: 50.1 bits (118), Expect = 1.810e-5
Identity = 23/38 (60.53%), Postives = 28/38 (73.68%), Query Frame = 1 
Query:   52 PRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQVM 165
            P  LP KR GLYDPA+E DSCGVG    +K +ASR++M
Sbjct:    6 PTRLPAKR-GLYDPAMEHDSCGVGFVANIKGIASREIM 42
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BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: A0A2A4R957_9GAMM (Glutamine amidotransferase type-2 domain-containing protein (Fragment) n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A2A4R957_9GAMM)

HSP 1 Score: 47.8 bits (112), Expect = 1.980e-5
Identity = 24/47 (51.06%), Postives = 31/47 (65.96%), Query Frame = 1 
Query:   22 RMPNRKPLAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQV 162
            ++P +  L  P +LP K+ GLYDPA EKDSCGVG    +K  AS Q+
Sbjct:   11 KLPEQTRLGLPGSLPDKQ-GLYDPAFEKDSCGVGFVAHIKGKASHQI 56
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BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: A0A2E6A6M5_9GAMM (Glutamate synthase large subunit n=2 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A2E6A6M5_9GAMM)

HSP 1 Score: 49.7 bits (117), Expect = 2.480e-5
Identity = 24/38 (63.16%), Postives = 28/38 (73.68%), Query Frame = 1 
Query:   52 PRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQVM 165
            P  LP KR GLYDPA+E DSCGVG    +K VASR++M
Sbjct:    6 PTGLPEKR-GLYDPAMEHDSCGVGFVANIKGVASREIM 42
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BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: A0A293NM27_9GAMM (Glutamate synthase large subunit n=2 Tax=Porticoccaceae bacterium TaxID=2026782 RepID=A0A293NM27_9GAMM)

HSP 1 Score: 49.7 bits (117), Expect = 2.480e-5
Identity = 28/49 (57.14%), Postives = 34/49 (69.39%), Query Frame = 1 
Query:   22 RMPNRKPLAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQVMM 168
            R+ NR+ + G    P KR GLYDPA EKDSCGVG   Q+K  ASRQ+M+
Sbjct:    3 RIKNRR-IDGRIGYPEKR-GLYDPAFEKDSCGVGFVAQIKGQASRQIML 49
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BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: A0A382DIB1_9ZZZZ (Glutamine amidotransferase type-2 domain-containing protein (Fragment) n=1 Tax=marine metagenome TaxID=408172 RepID=A0A382DIB1_9ZZZZ)

HSP 1 Score: 46.6 bits (109), Expect = 4.180e-5
Identity = 24/45 (53.33%), Postives = 30/45 (66.67%), Query Frame = 1 
Query:   31 NRKPLAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQVM 165
            N + + G    P K+ GLYDPALEKDSCGVG    +K VAS ++M
Sbjct:    2 NNERIDGRVGFPEKQ-GLYDPALEKDSCGVGFIANIKGVASHEIM 45
[Close]
BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: A0A424RLJ2_9GAMM (Glutamate synthase large subunit n=2 Tax=Cellvibrionales TaxID=1706369 RepID=A0A424RLJ2_9GAMM)

HSP 1 Score: 48.9 bits (115), Expect = 4.650e-5
Identity = 21/33 (63.64%), Postives = 26/33 (78.79%), Query Frame = 1 
Query:   70 KRVGLYDPALEKDSCGVGLAVQMKSVASRQVMM 168
            +R GLYDPA EKDSCGVG    +K +ASRQ+M+
Sbjct:   17 ERQGLYDPAFEKDSCGVGFVAHIKGLASRQIML 49
[Close]
BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Match: A0A3E0PRP2_9BACT (Glutamate synthase large subunit n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3E0PRP2_9BACT)

HSP 1 Score: 48.9 bits (115), Expect = 4.650e-5
Identity = 21/40 (52.50%), Postives = 26/40 (65.00%), Query Frame = 1 
Query:   43 LAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQV 162
            ++  R  P +R GLYDPA E DSCGVG    +K V SRQ+
Sbjct:    7 ISADRRFPLERTGLYDPAYEHDSCGVGFVAHIKGVTSRQI 46
The following BLAST results are available for this feature:
BLAST of mRNA_L-digitata_M_contig1007.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 13
Match NameE-valueIdentityDescription
D8LCE1_ECTSI4.500e-2287.72Glutamate synthase (NADH/NADPH-dependent), N-termi... [more]
A0A6H5KZS8_9PHAE1.170e-2085.96Glutamine amidotransferase type-2 domain-containin... [more]
A0A835YLR4_9STRA1.350e-866.67Glutamine amidotransferase type-2 domain-containin... [more]
A0A2E4UQE6_9GAMM1.810e-560.53Glutamate synthase large subunit n=1 Tax=Gammaprot... [more]
A0A2A4R957_9GAMM1.980e-551.06Glutamine amidotransferase type-2 domain-containin... [more]
A0A2E6A6M5_9GAMM2.480e-563.16Glutamate synthase large subunit n=2 Tax=Gammaprot... [more]
A0A293NM27_9GAMM2.480e-557.14Glutamate synthase large subunit n=2 Tax=Porticocc... [more]
A0A382DIB1_9ZZZZ4.180e-553.33Glutamine amidotransferase type-2 domain-containin... [more]
A0A424RLJ2_9GAMM4.650e-563.64Glutamate synthase large subunit n=2 Tax=Cellvibri... [more]
A0A3E0PRP2_9BACT4.650e-552.50Glutamate synthase large subunit n=1 Tax=Planctomy... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-digitata_M_contig1007contigL-digitata_M_contig1007:2983..3171 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Laminaria digitata LdigPH10_18mv male OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2903.EOD35180
Preferred nameGLT1
PFAMsFer4_20,GATase_2,GXGXG,Glu_syn_central,Glu_synthase,Pyr_redox_2
Model size189
Max annot lvl2759|Eukaryota
KEGG rclassRC00006,RC00010,RC02799
KEGG koko:K00264,ko:K00266,ko:K17792
KEGG TC3.A.8.1
KEGG ReactionR00093,R00114,R00248
KEGG Pathwayko00250,ko00910,ko01100,ko01110,ko01120,ko01130,ko01230,map00250,map00910,map01100,map01110,map01120,map01130,map01230
Hectar predicted targeting categoryno signal peptide or anchor
GOsGO:0001505,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0006082,GO:0006520,GO:0006536,GO:0006537,GO:0006541,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009064,GO:0009084,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009719,GO:0009725,GO:0009735,GO:0009987,GO:0010033,GO:0010035,GO:0010038,GO:0015930,GO:0016040,GO:0016053,GO:0016491,GO:0016638,GO:0016639,GO:0017144,GO:0019676,GO:0019740,GO:0019752,GO:0030447,GO:0032502,GO:0036267,GO:0040007,GO:0042133,GO:0042221,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0043648,GO:0043650,GO:0044182,GO:0044237,GO:0044238,GO:0044249,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0045181,GO:0046394,GO:0046686,GO:0048589,GO:0050896,GO:0055114,GO:0060359,GO:0065007,GO:0065008,GO:0070783,GO:0071704,GO:0097054,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607,GO:1901698
Exons1
Evalue1e-05
EggNOG OGsCOG0069@1|root,KOG0399@2759|Eukaryota
EC1.4.1.13,1.4.1.14
DescriptionL-glutamate biosynthetic process
Cds size165
COG categoryE
BRITEko00000,ko00001,ko01000,ko02000,ko03029
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682406609.3943822-CDS-L-digitata_M_contig1007:2982..31471682406609.3943822-CDS-L-digitata_M_contig1007:2982..3147Laminaria digitata LdigPH10_18mv maleCDSL-digitata_M_contig1007 2983..3147 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682406609.4065523-UTR-L-digitata_M_contig1007:3147..31711682406609.4065523-UTR-L-digitata_M_contig1007:3147..3171Laminaria digitata LdigPH10_18mv maleUTRL-digitata_M_contig1007 3148..3171 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-digitata_M_contig1007.2.1prot_L-digitata_M_contig1007.2.1Laminaria digitata LdigPH10_18mv malepolypeptideL-digitata_M_contig1007 2983..3147 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-digitata_M_contig1007.2.1

>prot_L-digitata_M_contig1007.2.1 ID=prot_L-digitata_M_contig1007.2.1|Name=mRNA_L-digitata_M_contig1007.2.1|organism=Laminaria digitata LdigPH10_18mv male|type=polypeptide|length=55bp
MPNRKPLAGPRTLPTKRVGLYDPALEKDSCGVGLAVQMKSVASRQVMMMK
MMIS*
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mRNA from alignment at L-digitata_M_contig1007:2983..3171-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-digitata_M_contig1007.2.1 ID=mRNA_L-digitata_M_contig1007.2.1|Name=mRNA_L-digitata_M_contig1007.2.1|organism=Laminaria digitata LdigPH10_18mv male|type=mRNA|length=189bp|location=Sequence derived from alignment at L-digitata_M_contig1007:2983..3171- (Laminaria digitata LdigPH10_18mv male)
GATGCGGCGGCTCGCGCGGCTCGTATGCCCAACAGGAAGCCCCTGGCCGG
GCCTCGCACGCTTCCCACGAAGCGCGTGGGGTTGTACGACCCTGCCCTGG
AGAAAGACTCGTGCGGCGTGGGGCTCGCGGTGCAGATGAAGAGCGTGGCA
TCTCGCCAGGTGATGATGATGAAGATGATGATATCATAG
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Coding sequence (CDS) from alignment at L-digitata_M_contig1007:2983..3171-

>mRNA_L-digitata_M_contig1007.2.1 ID=mRNA_L-digitata_M_contig1007.2.1|Name=mRNA_L-digitata_M_contig1007.2.1|organism=Laminaria digitata LdigPH10_18mv male|type=CDS|length=165bp|location=Sequence derived from alignment at L-digitata_M_contig1007:2983..3171- (Laminaria digitata LdigPH10_18mv male)
ATGCCCAACAGGAAGCCCCTGGCCGGGCCTCGCACGCTTCCCACGAAGCG
CGTGGGGTTGTACGACCCTGCCCTGGAGAAAGACTCGTGCGGCGTGGGGC
TCGCGGTGCAGATGAAGAGCGTGGCATCTCGCCAGGTGATGATGATGAAG
ATGATGATATCATAG
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