mRNA_L-digitata_M_contig100229.1.1 (mRNA) Laminaria digitata LdigPH10_18mv male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-digitata_M_contig100229.1.1
Unique NamemRNA_L-digitata_M_contig100229.1.1
TypemRNA
OrganismLaminaria digitata LdigPH10_18mv male (Laminaria digitata LdigPH10_18mv male (Oarweed))
Homology
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BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: A0A6H5KZP7_9PHAE (4-hydroxy-4-methyl-2-oxoglutarate aldolase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KZP7_9PHAE)

HSP 1 Score: 67.4 bits (163), Expect = 1.050e-12
Identity = 29/38 (76.32%), Postives = 34/38 (89.47%), Query Frame = 1 
Query:    4 GEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVPR 117
            GE+GVPV F GLRF+ GQ+VYADEDGIVV+ EPL+VPR
Sbjct:  144 GEQGVPVEFGGLRFKAGQFVYADEDGIVVSDEPLRVPR 181
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BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: D7FZC9_ECTSI (Regulator of ribonuclease activity A, RraA n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FZC9_ECTSI)

HSP 1 Score: 64.7 bits (156), Expect = 1.700e-11
Identity = 27/37 (72.97%), Postives = 33/37 (89.19%), Query Frame = 1 
Query:    7 EEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVPR 117
            E+GVPV F GLRF+ GQ+VYADEDGI+V+ EPL+VPR
Sbjct:  171 EQGVPVEFGGLRFKAGQFVYADEDGIIVSDEPLRVPR 207
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BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: UPI0004190DF4 (ribonuclease E activity regulator RraA n=1 Tax=Pseudomonas azotifigens TaxID=291995 RepID=UPI0004190DF4)

HSP 1 Score: 60.8 bits (146), Expect = 2.310e-10
Identity = 25/39 (64.10%), Postives = 32/39 (82.05%), Query Frame = 1 
Query:    1 IGEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVPR 117
            +GE GVPV F G+ FRPG+YVYAD +GIVV+ EPL++P 
Sbjct:  124 VGELGVPVTFCGVTFRPGEYVYADNNGIVVSPEPLQMPE 162
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BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: UPI0018ABF1E5 (ribonuclease E activity regulator RraA n=1 Tax=Pseudomonas fulva TaxID=47880 RepID=UPI0018ABF1E5)

HSP 1 Score: 59.3 bits (142), Expect = 9.240e-10
Identity = 23/39 (58.97%), Postives = 33/39 (84.62%), Query Frame = 1 
Query:    1 IGEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVPR 117
            IGE  VPV FAG+ FRPG+Y+YAD +G++V+T PL++P+
Sbjct:  125 IGEVDVPVTFAGVTFRPGEYLYADNNGVIVSTTPLEMPK 163
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BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: A0A5E6QVG3_PSEFL (4-hydroxy-4-methyl-2-oxoglutarate aldolase n=6 Tax=Pseudomonas TaxID=286 RepID=A0A5E6QVG3_PSEFL)

HSP 1 Score: 58.2 bits (139), Expect = 2.530e-9
Identity = 21/39 (53.85%), Postives = 31/39 (79.49%), Query Frame = 1 
Query:    1 IGEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVPR 117
            +GE  +PV FAG+ FRPG+Y+YAD +G++V+  PLK+P 
Sbjct:  124 VGELNIPVTFAGVTFRPGEYIYADNNGVIVSPSPLKMPE 162
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BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: A0A1T1HCE0_OCELI (4-hydroxy-4-methyl-2-oxoglutarate aldolase n=3 Tax=Oceanospirillum TaxID=965 RepID=A0A1T1HCE0_OCELI)

HSP 1 Score: 57.8 bits (138), Expect = 3.510e-9
Identity = 24/38 (63.16%), Postives = 31/38 (81.58%), Query Frame = 1 
Query:    1 IGEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVP 114
            IGEEG+ V F G+ FRPG+YVYAD +GI+V+ E LK+P
Sbjct:  124 IGEEGLDVTFGGVTFRPGEYVYADNNGIIVSPEALKMP 161
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BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: RRAAH_PSEU5 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase n=18 Tax=Pseudomonadales TaxID=72274 RepID=RRAAH_PSEU5)

HSP 1 Score: 57.4 bits (137), Expect = 5.010e-9
Identity = 23/39 (58.97%), Postives = 31/39 (79.49%), Query Frame = 1 
Query:    1 IGEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVPR 117
            IG+  VPV F G+ FRPG+YVYAD +GI+V+ EPL +P+
Sbjct:  124 IGDLNVPVTFCGVTFRPGEYVYADNNGIIVSPEPLSMPQ 162
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BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: A0A5R9QB07_9PSED (4-hydroxy-4-methyl-2-oxoglutarate aldolase n=3 Tax=Pseudomonas TaxID=286 RepID=A0A5R9QB07_9PSED)

HSP 1 Score: 57.0 bits (136), Expect = 7.050e-9
Identity = 23/38 (60.53%), Postives = 31/38 (81.58%), Query Frame = 1 
Query:    1 IGEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVP 114
            +G+  VPV F G+ FRPG+YVYAD +GI+V+ EPLK+P
Sbjct:  124 LGDLNVPVTFCGVTFRPGEYVYADNNGILVSPEPLKMP 161
[Close]
BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: A0A1Y5H2Q9_9GAMM (4-hydroxy-4-methyl-2-oxoglutarate aldolase n=1 Tax=Gammaproteobacteria bacterium 45_16_T64 TaxID=1856297 RepID=A0A1Y5H2Q9_9GAMM)

HSP 1 Score: 57.0 bits (136), Expect = 7.050e-9
Identity = 22/39 (56.41%), Postives = 30/39 (76.92%), Query Frame = 1 
Query:    1 IGEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVPR 117
            +GE  +PV F G+ F PG+YVYAD +GI+V+ EPLK+P 
Sbjct:  124 LGEMNIPVTFGGVTFTPGEYVYADNNGIIVSPEPLKLPE 162
[Close]
BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Match: RRAAH_AZOVD (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase n=178 Tax=Proteobacteria TaxID=1224 RepID=RRAAH_AZOVD)

HSP 1 Score: 56.6 bits (135), Expect = 9.920e-9
Identity = 22/39 (56.41%), Postives = 31/39 (79.49%), Query Frame = 1 
Query:    1 IGEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVPR 117
            IG+  VPV F G+ FRPG+Y+YAD +GI+V+ +PLK+P 
Sbjct:  124 IGDLNVPVTFGGVTFRPGEYLYADNNGIIVSPQPLKMPE 162
The following BLAST results are available for this feature:
BLAST of mRNA_L-digitata_M_contig100229.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KZP7_9PHAE1.050e-1276.324-hydroxy-4-methyl-2-oxoglutarate aldolase n=1 Tax... [more]
D7FZC9_ECTSI1.700e-1172.97Regulator of ribonuclease activity A, RraA n=1 Tax... [more]
UPI0004190DF42.310e-1064.10ribonuclease E activity regulator RraA n=1 Tax=Pse... [more]
UPI0018ABF1E59.240e-1058.97ribonuclease E activity regulator RraA n=1 Tax=Pse... [more]
A0A5E6QVG3_PSEFL2.530e-953.854-hydroxy-4-methyl-2-oxoglutarate aldolase n=6 Tax... [more]
A0A1T1HCE0_OCELI3.510e-963.164-hydroxy-4-methyl-2-oxoglutarate aldolase n=3 Tax... [more]
RRAAH_PSEU55.010e-958.97Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolas... [more]
A0A5R9QB07_9PSED7.050e-960.534-hydroxy-4-methyl-2-oxoglutarate aldolase n=3 Tax... [more]
A0A1Y5H2Q9_9GAMM7.050e-956.414-hydroxy-4-methyl-2-oxoglutarate aldolase n=1 Tax... [more]
RRAAH_AZOVD9.920e-956.41Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolas... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-digitata_M_contig100229contigL-digitata_M_contig100229:154..282 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Laminaria digitata LdigPH10_18mv male OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog1114970.PSF113_1764
Preferred namerraA
PFAMsRraA-like
Model size129
Max annot lvl1236|Gammaproteobacteria
KEGG koko:K02553
Hectar predicted targeting categoryno signal peptide or anchor
Exons1
Evalue1.08e-10
EggNOG OGsCOG0684@1|root,COG0684@2|Bacteria,1RH18@1224|Proteobacteria,1RS9U@1236|Gammaproteobacteria,1YM1N@136843|Pseudomonas fluorescens group
DescriptionCatalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions
Cds size129
COG categoryH
BRITEko00000,ko03019
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682406605.216405-CDS-L-digitata_M_contig100229:153..2821682406605.216405-CDS-L-digitata_M_contig100229:153..282Laminaria digitata LdigPH10_18mv maleCDSL-digitata_M_contig100229 154..282 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-digitata_M_contig100229.1.1prot_L-digitata_M_contig100229.1.1Laminaria digitata LdigPH10_18mv malepolypeptideL-digitata_M_contig100229 154..282 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-digitata_M_contig100229.1.1

>prot_L-digitata_M_contig100229.1.1 ID=prot_L-digitata_M_contig100229.1.1|Name=mRNA_L-digitata_M_contig100229.1.1|organism=Laminaria digitata LdigPH10_18mv male|type=polypeptide|length=43bp
IGEEGVPVNFAGLRFRPGQYVYADEDGIVVATEPLKVPRPGL*
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mRNA from alignment at L-digitata_M_contig100229:154..282+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-digitata_M_contig100229.1.1 ID=mRNA_L-digitata_M_contig100229.1.1|Name=mRNA_L-digitata_M_contig100229.1.1|organism=Laminaria digitata LdigPH10_18mv male|type=mRNA|length=129bp|location=Sequence derived from alignment at L-digitata_M_contig100229:154..282+ (Laminaria digitata LdigPH10_18mv male)
ATAGGGGAAGAAGGAGTACCGGTGAATTTTGCGGGATTACGGTTCCGGCC
AGGGCAGTACGTCTACGCGGACGAGGACGGCATCGTTGTGGCGACAGAGC
CGTTGAAGGTTCCCCGCCCCGGGCTGTGA
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Coding sequence (CDS) from alignment at L-digitata_M_contig100229:154..282+

>mRNA_L-digitata_M_contig100229.1.1 ID=mRNA_L-digitata_M_contig100229.1.1|Name=mRNA_L-digitata_M_contig100229.1.1|organism=Laminaria digitata LdigPH10_18mv male|type=CDS|length=129bp|location=Sequence derived from alignment at L-digitata_M_contig100229:154..282+ (Laminaria digitata LdigPH10_18mv male)
ATAGGGGAAGAAGGAGTACCGGTGAATTTTGCGGGATTACGGTTCCGGCC
AGGGCAGTACGTCTACGCGGACGAGGACGGCATCGTTGTGGCGACAGAGC
CGTTGAAGGTTCCCCGCCCCGGGCTGTGA
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