mRNA_H-elongata_contig102821.244.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig102821.244.1
Unique NamemRNA_H-elongata_contig102821.244.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: D7FWF1_ECTSI (tRNA pseudouridine synthase B n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FWF1_ECTSI)

HSP 1 Score: 147 bits (370), Expect = 1.290e-43
Identity = 70/84 (83.33%), Postives = 75/84 (89.29%), Query Frame = 1 
Query:    4 QVEREARPVTVHSLRQVDPALPLPRLGLEVECGGGFYVRTLVEDLGRVLGTRAHMTALERTKQGPFELKHALERDEWEYNELCK 255
            QVEREAR V V SLRQ DPALPLP LGLE+ECGGGFYVRTL+EDLGR LGTRAHMTALERTKQGPFEL+HAL  +EWEY+ LCK
Sbjct:   29 QVEREARQVRVDSLRQADPALPLPLLGLELECGGGFYVRTLIEDLGRALGTRAHMTALERTKQGPFELQHALRLEEWEYDRLCK 112
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BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: A0A6H5KMC7_9PHAE (tRNA pseudouridine(55) synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KMC7_9PHAE)

HSP 1 Score: 146 bits (368), Expect = 1.590e-42
Identity = 69/85 (81.18%), Postives = 75/85 (88.24%), Query Frame = 1 
Query:    1 VQVEREARPVTVHSLRQVDPALPLPRLGLEVECGGGFYVRTLVEDLGRVLGTRAHMTALERTKQGPFELKHALERDEWEYNELCK 255
            + VEREAR V V SLRQ DPALPLP LGLE+ECGGGFYVRTL+EDLGR LGTRAHMTALERTKQGPFEL+HAL  +EWEY+ LCK
Sbjct:  106 ITVEREARQVRVDSLRQADPALPLPLLGLELECGGGFYVRTLIEDLGRALGTRAHMTALERTKQGPFELRHALRLEEWEYDRLCK 190
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BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: A0A835Z9D0_9STRA (tRNA pseudouridine(55) synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9D0_9STRA)

HSP 1 Score: 95.1 bits (235), Expect = 6.330e-22
Identity = 41/81 (50.62%), Postives = 56/81 (69.14%), Query Frame = 1 
Query:    7 VEREARPVTVHSLRQVDPALPLPRLGLEVECGGGFYVRTLVEDLGRVLGTRAHMTALERTKQGPFELKHALERDEWEYNEL 249
            VER+ARPVT++ L      + LP   L V+C GG Y+RTL+ D+ R LGTR HMT+L RTKQGPF L+H L++  W + ++
Sbjct:  176 VERDARPVTIYQLEAPAEGVALPSFALNVQCSGGTYIRTLIADIARALGTRGHMTSLVRTKQGPFGLEHCLDQQHWHFEQI 256
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BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: UPI0014557746 (probable tRNA pseudouridine synthase 1 isoform X1 n=2 Tax=Asterias rubens TaxID=7604 RepID=UPI0014557746)

HSP 1 Score: 91.3 bits (225), Expect = 3.240e-20
Identity = 46/84 (54.76%), Postives = 59/84 (70.24%), Query Frame = 1 
Query:   13 REARPVTVHSLR--QVDPALPLPRLGLEVECGGGFYVRTLVEDLGRVLGTRAHMTALERTKQGPFELKHALE-RDEWEYNELCK 255
            + ARPVTVH+L+  Q  P    P   L+V CGGGFYVR L+ DLG+VLGT AH+  LERT+QGPF L+H L+  +EW +  +CK
Sbjct:  216 KPARPVTVHNLKCLQFSP----PYFTLDVYCGGGFYVRQLIHDLGKVLGTCAHLVELERTQQGPFTLQHVLQTEEEWTFQNICK 295
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BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: A0A4W3IJ22_CALMI (tRNA pseudouridine(55) synthase n=6 Tax=Callorhinchus milii TaxID=7868 RepID=A0A4W3IJ22_CALMI)

HSP 1 Score: 88.2 bits (217), Expect = 2.300e-19
Identity = 47/82 (57.32%), Postives = 58/82 (70.73%), Query Frame = 1 
Query:   13 REARPVTVHSLRQVDPALPLPRLGLEVECGGGFYVRTLVEDLGRVLGTRAHMTALERTKQGPFELK-HALERDEWEYNELCK 255
            R ARPV VHSL   D + PL    LE+ECGGGFYVR+LV DLG+ L + AH+  LERTKQGPF L+ HAL  ++W  +E+ K
Sbjct:  119 RPARPVVVHSLSVQDFSPPL--FTLEIECGGGFYVRSLVSDLGKELSSCAHVKELERTKQGPFTLEEHALPEEKWTIDEIAK 198
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BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: F0YN11_AURAN (tRNA pseudouridine(55) synthase (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YN11_AURAN)

HSP 1 Score: 85.5 bits (210), Expect = 3.100e-19
Identity = 44/94 (46.81%), Postives = 59/94 (62.77%), Query Frame = 1 
Query:    1 VQVEREARPVTVHSLRQVDPA-------LPLPRLGLEVECGGGFYVRTLVEDLGR--VLGTRAHMTALERTKQGPFELKHALERDEWEYNELCK 255
            ++VERE+RPVT+  L   D         L LP  GL+VEC GG Y+RTL+ DL R   + + AHM  LERTKQGPFEL   L   +W+++ +C+
Sbjct:   53 IEVERESRPVTISRLALDDRGVGAVAGPLGLPHFGLDVECSGGTYIRTLISDLARHEAVKSVAHMVELERTKQGPFELGDCLHEADWDFDAICQ 146
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BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: A0A8B7YYF7_ACAPL (tRNA pseudouridine(55) synthase n=1 Tax=Acanthaster planci TaxID=133434 RepID=A0A8B7YYF7_ACAPL)

HSP 1 Score: 87.4 bits (215), Expect = 5.140e-19
Identity = 44/82 (53.66%), Postives = 55/82 (67.07%), Query Frame = 1 
Query:   13 REARPVTVHSLRQVDPALPLPRLGLEVECGGGFYVRTLVEDLGRVLGTRAHMTALERTKQGPFEL-KHALERDEWEYNELCK 255
            + ARPVTVHSL+ V+     P   L+V CGGGFYVR L+EDLG+ L T AH+  L RT+QGPF L  HAL  D W +  +C+
Sbjct:  173 KPARPVTVHSLKCVE--FSPPYFTLDVHCGGGFYVRQLIEDLGQDLRTNAHLAELTRTQQGPFTLANHALAEDRWTFEHICR 252
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BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: UPI0018D95F47 (probable tRNA pseudouridine synthase 1 n=1 Tax=Patiria miniata TaxID=46514 RepID=UPI0018D95F47)

HSP 1 Score: 87.0 bits (214), Expect = 8.020e-19
Identity = 43/82 (52.44%), Postives = 55/82 (67.07%), Query Frame = 1 
Query:   13 REARPVTVHSLRQVDPALPLPRLGLEVECGGGFYVRTLVEDLGRVLGTRAHMTALERTKQGPFEL-KHALERDEWEYNELCK 255
            + ARPVTVHSL  V      P   L++ CGGGFYVR L++DLG+ +GT AH+  L RT+QGPF L KHAL  D+W    +C+
Sbjct:  180 KPARPVTVHSLECVQ--FSPPYFTLDIHCGGGFYVRQLIQDLGQDMGTNAHLAELTRTQQGPFTLAKHALAEDKWTLEHICE 259
[Close]
BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: F0YN09_AURAN (tRNA pseudouridine(55) synthase (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YN09_AURAN)

HSP 1 Score: 85.5 bits (210), Expect = 1.670e-18
Identity = 44/94 (46.81%), Postives = 59/94 (62.77%), Query Frame = 1 
Query:    1 VQVEREARPVTVHSLRQVDPA-------LPLPRLGLEVECGGGFYVRTLVEDLGR--VLGTRAHMTALERTKQGPFELKHALERDEWEYNELCK 255
            ++VERE+RPVT+  L   D         L LP  GL+VEC GG Y+RTL+ DL R   + + AHM  LERTKQGPFEL   L   +W+++ +C+
Sbjct:  144 IEVERESRPVTISRLALDDRGVGAVAGPLGLPHFGLDVECSGGTYIRTLISDLARHEAVKSVAHMVELERTKQGPFELGDCLHEADWDFDAICQ 237
[Close]
BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Match: A0A8J2SXF4_9STRA (tRNA pseudouridine(55) synthase n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SXF4_9STRA)

HSP 1 Score: 86.3 bits (212), Expect = 1.860e-18
Identity = 45/93 (48.39%), Postives = 60/93 (64.52%), Query Frame = 1 
Query:    1 VQVEREARPVTVHSLRQVDPA--------LPLPRLGLEVECGGGFYVRTLVEDLGRVLGTRAHMTALERTKQGPFELKHALERDEW-EYNELC 252
            ++VEREAR V+V  LR VD          L LP  GL+VEC GG Y+RTL++DL R  G+  HM  LERT+QGPF L+H L  + W +++ +C
Sbjct:  184 LEVEREARQVSVSRLRMVDDRGVGSHEAPLSLPFFGLDVECSGGTYIRTLIDDLAREAGSCGHMVELERTRQGPFGLEHCLGDEAWGDFDAVC 276
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig102821.244.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FWF1_ECTSI1.290e-4383.33tRNA pseudouridine synthase B n=1 Tax=Ectocarpus s... [more]
A0A6H5KMC7_9PHAE1.590e-4281.18tRNA pseudouridine(55) synthase n=1 Tax=Ectocarpus... [more]
A0A835Z9D0_9STRA6.330e-2250.62tRNA pseudouridine(55) synthase n=1 Tax=Tribonema ... [more]
UPI00145577463.240e-2054.76probable tRNA pseudouridine synthase 1 isoform X1 ... [more]
A0A4W3IJ22_CALMI2.300e-1957.32tRNA pseudouridine(55) synthase n=6 Tax=Callorhinc... [more]
F0YN11_AURAN3.100e-1946.81tRNA pseudouridine(55) synthase (Fragment) n=1 Tax... [more]
A0A8B7YYF7_ACAPL5.140e-1953.66tRNA pseudouridine(55) synthase n=1 Tax=Acanthaste... [more]
UPI0018D95F478.020e-1952.44probable tRNA pseudouridine synthase 1 n=1 Tax=Pat... [more]
F0YN09_AURAN1.670e-1846.81tRNA pseudouridine(55) synthase (Fragment) n=1 Tax... [more]
A0A8J2SXF4_9STRA1.860e-1848.39tRNA pseudouridine(55) synthase n=1 Tax=Pelagomona... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig102821contigH-elongata_contig102821:221..775 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score148.7
Seed ortholog evalue1.3e-33
Seed eggNOG ortholog2880.D7FWF1
Preferred nameTRUB1
KEGG koko:K03177
GOsGO:0000049,GO:0001522,GO:0003674,GO:0003676,GO:0003723,GO:0003824,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005829,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0009451,GO:0009982,GO:0009987,GO:0010467,GO:0016070,GO:0016071,GO:0016556,GO:0016853,GO:0016866,GO:0031119,GO:0034337,GO:0034470,GO:0034641,GO:0034660,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0071704,GO:0090304,GO:0097159,GO:1901360,GO:1901363,GO:1990481
EggNOG free text desc.mRNA pseudouridine synthesis
EggNOG OGsCOG0130@1,KOG2529@2759
EC5.4.99.25
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03016
Hectar predicted targeting categoryother localisation
Exons2
Model size255
Cds size255
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929565.3125408-CDS-H-elongata_contig102821:220..4121622929565.3125408-CDS-H-elongata_contig102821:220..412Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig102821 221..412 +
1691679072.6745214-CDS-H-elongata_contig102821:220..4121691679072.6745214-CDS-H-elongata_contig102821:220..412Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig102821 221..412 +
1622929565.3357072-CDS-H-elongata_contig102821:712..7751622929565.3357072-CDS-H-elongata_contig102821:712..775Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig102821 713..775 +
1691679072.6939015-CDS-H-elongata_contig102821:712..7751691679072.6939015-CDS-H-elongata_contig102821:712..775Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig102821 713..775 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig102821.244.1prot_H-elongata_contig102821.244.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig102821 221..775 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig102821.244.1

>prot_H-elongata_contig102821.244.1 ID=prot_H-elongata_contig102821.244.1|Name=mRNA_H-elongata_contig102821.244.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=85bp
VQVEREARPVTVHSLRQVDPALPLPRLGLEVECGGGFYVRTLVEDLGRVL
GTRAHMTALERTKQGPFELKHALERDEWEYNELCK
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mRNA from alignment at H-elongata_contig102821:221..775+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig102821.244.1 ID=mRNA_H-elongata_contig102821.244.1|Name=mRNA_H-elongata_contig102821.244.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=555bp|location=Sequence derived from alignment at H-elongata_contig102821:221..775+ (Himanthalia elongata Himel1 dioecious)
GTCCAGGTGGAGCGAGAGGCCCGGCCAGTTACGGTGCACTCCCTCCGGCA
AGTCGACCCAGCCTTGCCGCTACCGCGATTGGGGTTGGAAGTGGAATGCG
GCGGTGGATTCTACGTTCGAACGCTGGTGGAAGACCTCGGGAGGGTGCTC
GGCACGAGAGCGCACATGACCGCTCTCGAGAGAACAAAGCAGGTCGACCT
TTTTTTAGCTACTAAGCTTGGCAAGGTCTCACACGTCTCTGCGCACTCAA
GAGTTTTTTGTGCGCATGCTCTAACTGCGATCGTCTCGTGACCACTGGCA
TGATGTGTGTGGTCATCATCGTCGTATGATTCGGCGCTTCTAGAGTCTAG
GGAAGCCCCTCGGTCGCTCAGCAATCAGCATTTGTCATCGAAAACTTTCG
TGTTGCCAGGTATGTTGTAAGCTACGATATTTTCCTTTCCCGCCCGTTCT
TCGCGCTGCTCGTGTGGTCACCGACTCTTTCCTTTCAAACAGGGGCCATT
CGAACTCAAACACGCTTTGGAACGAGACGAGTGGGAGTACAACGAGCTTT
GCAAG
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Coding sequence (CDS) from alignment at H-elongata_contig102821:221..775+

>mRNA_H-elongata_contig102821.244.1 ID=mRNA_H-elongata_contig102821.244.1|Name=mRNA_H-elongata_contig102821.244.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=510bp|location=Sequence derived from alignment at H-elongata_contig102821:221..775+ (Himanthalia elongata Himel1 dioecious)
GTCCAGGTGGAGCGAGAGGCCCGGCCAGTTACGGTGCACTCCCTCCGGCA
AGTCGACCCAGCCTTGCCGCTACCGCGATTGGGGTTGGAAGTGGAATGCG
GCGGTGGATTCTACGTTCGAACGCTGGTGGAAGACCTCGGGAGGGTGCTC
GGCACGAGAGCGCACATGACCGCTCTCGAGAGAACAAAGCAGGTCCAGGT
GGAGCGAGAGGCCCGGCCAGTTACGGTGCACTCCCTCCGGCAAGTCGACC
CAGCCTTGCCGCTACCGCGATTGGGGTTGGAAGTGGAATGCGGCGGTGGA
TTCTACGTTCGAACGCTGGTGGAAGACCTCGGGAGGGTGCTCGGCACGAG
AGCGCACATGACCGCTCTCGAGAGAACAAAGCAGGGGCCATTCGAACTCA
AACACGCTTTGGAACGAGACGAGTGGGAGTACAACGAGCTTTGCAAGGGG
CCATTCGAACTCAAACACGCTTTGGAACGAGACGAGTGGGAGTACAACGA
GCTTTGCAAG
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