mRNA_H-elongata_contig117119.1338.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig117119.1338.1
Unique NamemRNA_H-elongata_contig117119.1338.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: A0A6H5JJR2_9PHAE (DNA topoisomerase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JJR2_9PHAE)

HSP 1 Score: 114 bits (285), Expect = 4.070e-30
Identity = 49/60 (81.67%), Postives = 56/60 (93.33%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYVKD 180
            YN IFEF+NC MMGQ V+MKITSVTGHLME EFAEPYG++WGAC+P+ELFTAP+IRYVKD
Sbjct:   37 YNPIFEFDNCSMMGQSVNMKITSVTGHLMELEFAEPYGKKWGACQPIELFTAPLIRYVKD 96
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BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: A0A4D9AQC4_SALSN (DNA topoisomerase n=1 Tax=Salvia splendens TaxID=180675 RepID=A0A4D9AQC4_SALSN)

HSP 1 Score: 67.0 bits (162), Expect = 5.080e-12
Identity = 32/58 (55.17%), Postives = 41/58 (70.69%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYV 174
            YN+IFEF NC +  Q + M  TSVTGHLME EF + Y R+W  C PV+L+ AP+ +YV
Sbjct:   10 YNRIFEF-NCVVRSQLIQMSFTSVTGHLMELEFDDRY-RKWHHCDPVDLYHAPVKKYV 65
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BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: A0A8J6CBF4_DIALT (DNA topoisomerase n=1 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J6CBF4_DIALT)

HSP 1 Score: 68.6 bits (166), Expect = 9.010e-12
Identity = 37/78 (47.44%), Postives = 50/78 (64.10%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYVKDVRDPIRAN-----RRAQE 219
            YN  F F+     G+R  M +TSVTGHLME++FAE + R W +C PV LFTAP+++YV + +  I+ N     RRA E
Sbjct:   34 YNACFGFDYDFGNGRRAQMVVTSVTGHLMESDFAESF-RGWHSCDPVALFTAPVVKYVPEDKVDIKTNLENEARRADE 110
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BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: UPI0020098337 (DNA topoisomerase 3-alpha isoform X1 n=4 Tax=Salvia TaxID=21880 RepID=UPI0020098337)

HSP 1 Score: 68.2 bits (165), Expect = 1.220e-11
Identity = 32/58 (55.17%), Postives = 42/58 (72.41%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYV 174
            YN+IFEF NC +  Q + M  TSVTGHLME EF + Y R+W +C PV+L+ AP+ +YV
Sbjct:   47 YNRIFEF-NCVVRSQLIQMSFTSVTGHLMELEFDDRY-RKWHSCDPVDLYHAPVKKYV 102
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BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: S8DSX4_9LAMI (DNA topoisomerase (Fragment) n=1 Tax=Genlisea aurea TaxID=192259 RepID=S8DSX4_9LAMI)

HSP 1 Score: 62.0 bits (149), Expect = 8.330e-11
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYV 174
            +N+IFEF +  +  Q   M  TSVTGHLME EFAE Y R+W +C PV+L+ AP+ ++V
Sbjct:   44 FNKIFEF-SYSIQNQPFHMSFTSVTGHLMELEFAERY-RKWHSCDPVDLYNAPVKKFV 99
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BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: W4FMS7_9STRA (DNA topoisomerase n=2 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FMS7_9STRA)

HSP 1 Score: 65.5 bits (158), Expect = 1.090e-10
Identity = 39/75 (52.00%), Postives = 45/75 (60.00%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYVKDVRDPIRANRRAQEKR 225
            YN IFEF    +  Q V M  TSVTGHLME EFA P  R W +C PVELFTA I + V+  + PI A  R +  R
Sbjct:   34 YNAIFEFPY-QINNQNVDMAFTSVTGHLMELEFA-PSHRSWKSCDPVELFTAKIEKKVRGDQKPIEATLRTEAAR 106
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BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: A0A0A0LBG7_CUCSA (DNA topoisomerase n=14 Tax=Cucurbitaceae TaxID=3650 RepID=A0A0A0LBG7_CUCSA)

HSP 1 Score: 65.1 bits (157), Expect = 1.490e-10
Identity = 32/60 (53.33%), Postives = 43/60 (71.67%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYVKD 180
            YN+IFEF N  + GQ  +M +TSVTGHLME  F E Y R+W +C PV+LF AP+ ++V +
Sbjct:   41 YNKIFEF-NYAIRGQSCNMIVTSVTGHLMELAFEERY-RKWHSCDPVQLFHAPVHKFVPE 98
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BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: A0A5A7TKA3_CUCME (DNA topoisomerase n=3 Tax=Benincaseae TaxID=1003877 RepID=A0A5A7TKA3_CUCME)

HSP 1 Score: 64.7 bits (156), Expect = 2.030e-10
Identity = 32/60 (53.33%), Postives = 43/60 (71.67%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYVKD 180
            YN+IFEF N  + GQ  +M +TSVTGHLME  F E Y R+W +C PV+LF AP+ ++V +
Sbjct:   41 YNKIFEF-NYAIRGQSCNMIVTSVTGHLMELAFEERY-RKWYSCDPVQLFHAPVHKFVPE 98
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BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: A0A5J5BVM9_9ASTE (DNA topoisomerase n=2 Tax=Nyssaceae TaxID=4289 RepID=A0A5J5BVM9_9ASTE)

HSP 1 Score: 63.5 bits (153), Expect = 5.180e-10
Identity = 31/60 (51.67%), Postives = 42/60 (70.00%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYVKD 180
            YN+IFEF N  + GQ   M  TSVTGHLME EF + Y R+W +C PV+L+ AP+ ++V +
Sbjct:   41 YNKIFEF-NYTISGQPCQMLFTSVTGHLMELEFEDRY-RKWHSCDPVDLYHAPVRKFVPE 98
[Close]
BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Match: A0A6P6T8K0_COFAR (DNA topoisomerase n=3 Tax=Coffea TaxID=13442 RepID=A0A6P6T8K0_COFAR)

HSP 1 Score: 63.5 bits (153), Expect = 5.180e-10
Identity = 32/66 (48.48%), Postives = 45/66 (68.18%), Query Frame = 1 
Query:    1 YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELFTAPIIRYVKDVRDPIR 198
            YN+IFEF N  + GQ   M  TSVTGHLME +F + + RRW +C PV+L+ AP+ ++V + +  IR
Sbjct:   53 YNKIFEF-NHTVNGQPCHMSFTSVTGHLMELDFDDRF-RRWHSCDPVDLYRAPVRKFVPEDKSDIR 116
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig117119.1338.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JJR2_9PHAE4.070e-3081.67DNA topoisomerase n=1 Tax=Ectocarpus sp. CCAP 1310... [more]
A0A4D9AQC4_SALSN5.080e-1255.17DNA topoisomerase n=1 Tax=Salvia splendens TaxID=1... [more]
A0A8J6CBF4_DIALT9.010e-1247.44DNA topoisomerase n=1 Tax=Diacronema lutheri TaxID... [more]
UPI00200983371.220e-1155.17DNA topoisomerase 3-alpha isoform X1 n=4 Tax=Salvi... [more]
S8DSX4_9LAMI8.330e-1151.72DNA topoisomerase (Fragment) n=1 Tax=Genlisea aure... [more]
W4FMS7_9STRA1.090e-1052.00DNA topoisomerase n=2 Tax=Aphanomyces astaci TaxID... [more]
A0A0A0LBG7_CUCSA1.490e-1053.33DNA topoisomerase n=14 Tax=Cucurbitaceae TaxID=365... [more]
A0A5A7TKA3_CUCME2.030e-1053.33DNA topoisomerase n=3 Tax=Benincaseae TaxID=100387... [more]
A0A5J5BVM9_9ASTE5.180e-1051.67DNA topoisomerase n=2 Tax=Nyssaceae TaxID=4289 Rep... [more]
A0A6P6T8K0_COFAR5.180e-1048.48DNA topoisomerase n=3 Tax=Coffea TaxID=13442 RepID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig117119contigH-elongata_contig117119:110..334 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score65.5
Seed ortholog evalue1.2e-08
Seed eggNOG ortholog3694.POPTR_0005s06810.1
Preferred nameTOP3A
KEGG koko:K03165
KEGG Pathwayko03440,ko03460,map03440,map03460
KEGG ModuleM00414
GOsGO:0000003,GO:0003674,GO:0003676,GO:0003677,GO:0003824,GO:0003916,GO:0003917,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0006139,GO:0006259,GO:0006260,GO:0006265,GO:0006725,GO:0006807,GO:0006996,GO:0007049,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0016043,GO:0016604,GO:0016605,GO:0016853,GO:0022414,GO:0031974,GO:0031981,GO:0034641,GO:0034645,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044451,GO:0044464,GO:0046483,GO:0051276,GO:0051321,GO:0070013,GO:0071103,GO:0071704,GO:0071840,GO:0090304,GO:0097159,GO:0140097,GO:1901360,GO:1901363,GO:1901576
EggNOG free text desc.Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
EggNOG OGs37IA6@33090,3G9US@35493,4JM73@91835,COG0550@1,KOG1956@2759
EC5.99.1.2
COG Functional cat.L
Best tax levelfabids
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03032,ko03400
Hectar predicted targeting categoryother localisation
Exons1
Model size225
Cds size225
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929681.4596686-CDS-H-elongata_contig117119:109..3341622929681.4596686-CDS-H-elongata_contig117119:109..334Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig117119 110..334 -
1691679117.5653534-CDS-H-elongata_contig117119:109..3341691679117.5653534-CDS-H-elongata_contig117119:109..334Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig117119 110..334 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig117119.1338.1prot_H-elongata_contig117119.1338.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig117119 110..334 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig117119.1338.1

>prot_H-elongata_contig117119.1338.1 ID=prot_H-elongata_contig117119.1338.1|Name=mRNA_H-elongata_contig117119.1338.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=75bp
YNQIFEFENCPMMGQRVSMKITSVTGHLMETEFAEPYGRRWGACRPVELF
TAPIIRYVKDVRDPIRANRRAQEKR
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mRNA from alignment at H-elongata_contig117119:110..334-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig117119.1338.1 ID=mRNA_H-elongata_contig117119.1338.1|Name=mRNA_H-elongata_contig117119.1338.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=225bp|location=Sequence derived from alignment at H-elongata_contig117119:110..334- (Himanthalia elongata Himel1 dioecious)
TACAATCAGATCTTCGAATTCGAAAACTGCCCGATGATGGGCCAGAGGGT
GAGTATGAAGATCACCTCGGTCACCGGCCACCTGATGGAAACGGAGTTCG
CGGAACCCTACGGGAGGCGCTGGGGCGCCTGTCGCCCCGTGGAGCTCTTC
ACGGCACCGATCATTCGCTACGTCAAGGACGTGAGGGATCCCATCCGTGC
CAACCGTCGAGCACAGGAGAAAAGG
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Coding sequence (CDS) from alignment at H-elongata_contig117119:110..334-

>mRNA_H-elongata_contig117119.1338.1 ID=mRNA_H-elongata_contig117119.1338.1|Name=mRNA_H-elongata_contig117119.1338.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=450bp|location=Sequence derived from alignment at H-elongata_contig117119:110..334- (Himanthalia elongata Himel1 dioecious)
TACAATCAGATCTTCGAATTCGAAAACTGCCCGATGATGGGCCAGAGGGT
GAGTATGAAGATCACCTCGGTCACCGGCCACCTGATGGAAACGGAGTTCG
CGGAACCCTACGGGAGGCGCTGGGGCGCCTGTCGCCCCGTGGAGCTCTTC
ACGGCACCGATCATTCGCTACGTCAAGGACGTGAGGGATCCCATCCGTGC
CAACCGTCGAGCACAGGAGAAAAGGTACAATCAGATCTTCGAATTCGAAA
ACTGCCCGATGATGGGCCAGAGGGTGAGTATGAAGATCACCTCGGTCACC
GGCCACCTGATGGAAACGGAGTTCGCGGAACCCTACGGGAGGCGCTGGGG
CGCCTGTCGCCCCGTGGAGCTCTTCACGGCACCGATCATTCGCTACGTCA
AGGACGTGAGGGATCCCATCCGTGCCAACCGTCGAGCACAGGAGAAAAGG
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