mRNA_H-elongata_contig84139.16025.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig84139.16025.1
Unique NamemRNA_H-elongata_contig84139.16025.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig84139.16025.1 vs. uniprot
Match: A0A6H5KHQ5_9PHAE (FAD-binding PCMH-type domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KHQ5_9PHAE)

HSP 1 Score: 180 bits (457), Expect = 2.450e-45
Identity = 155/460 (33.70%), Postives = 181/460 (39.35%), Query Frame = 1 
Query:  157 ADADSEVVIRNLAWATHPALGRRCPVGGISTRHSQAEYYGSV---GEGMALVVTTPEEE----EAQREEEGRMPSFRDISSLLYDYAVLRGLRPSLEALTSRTSILLAS---------------------------------------------------KPSADRVNLRGFS-AGCERTWDGDAAPSESTSKSSVFETMHFFLDRLPVWLLRGLAIIVRRLPRSRRTNCYRTEGLSRPPPAASCNRLAEGGEGLERFSNMAVAETDQEAREKCLAGDGEGTVMIASGSSVAVEDEVIADMDADAGANNSGGVDRLVEARGRALPRICEPGTPQSTKRCGPGVDIIALVERLDRLTALPSVYEPFTWQRYFFDSALRVPRALTPEGYALEASIASELLIQNLEGGLRCGDANTKASPERRDEVLQELALQIKTHRAGGLRCGVSDDRLYREEEEDACPEG 1359
            A++  +VVIRNLAWATHP LG       ++ +   A   G V   G GMA VV     E          EGRMPSFR+IS LLYDYAVLRG+RPSLEAL  R+S   AS                                                    P+A    L+G   AG      G   PS        F+ MH  LDR PVW L+GLA +  R+            G+              GG    R                                                               GR  P I                   A + RLD+L ALPSVYE FTWQ YFFDSALRVP  L P GYALE ++ASELL ++L GG   G    KASPERR  +L+EL LQI+THR+GGLRCG   D           PEG
Sbjct:   76 AESRPDVVIRNLAWATHPVLGLGDSSAIVARKRDPAVCGGGVAQEGGGMADVVAGRAAECTAAVGSGAREGRMPSFREISGLLYDYAVLRGMRPSLEALAMRSSFAAASVSPPPATPPGTPCVAHSGDTSGGRWSSSPSCPQSPYXXXXXGGSGEGRRGNPPTAGDGGLQGRGEAGLLHLRGGGLKPS---LPRLAFDAMHLLLDRSPVWALQGLAALAERV------------GVF-------------GGSSGHRHGXXXXXXX-------------------------------------------------XXXXXGRRRPGIS------------------ATLRRLDKLAALPSVYETFTWQVYFFDSALRVPEGLKPAGYALETALASELLQRSLPGGAEEG---AKASPERRQSILRELGLQIRTHRSGGLRCGGRGDDTRTSLRAGGAPEG 437
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BLAST of mRNA_H-elongata_contig84139.16025.1 vs. uniprot
Match: D7FUE9_ECTSI (Alkylglycerone-phosphate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUE9_ECTSI)

HSP 1 Score: 174 bits (441), Expect = 3.180e-43
Identity = 151/458 (32.97%), Postives = 185/458 (40.39%), Query Frame = 1 
Query:  157 ADADSEVVIRNLAWATHPALGRRCPVGGISTRHSQAEYYGSVGEG----MALVVT--TPEEEEA--QREEEGRMPSFRDISSLLYDYAVLRGLRPSLEALTSRTSILLAS--KPSADRVNLRGFSAGCERTWDG------------DAAPSESTSKSS-----------------------------------VFETMHFFLDRLPVWLLRGLAIIVRRLPRSRRTNCYRTEGLSRPPPAASCNRLAEGGEGLERFSNMAVAETDQEAREKCLAGDGEGTVMIASGSSVAVEDEVIADMDADAGANNSGGVDRLVEARGRALPRICEPGTPQSTKRCGPGVDIIALVERLDRLTALPSVYEPFTWQRYFFDSALRVPRALTPEGYALEASIASELLIQNLEGGLRCGDANTKASPERRDEVLQELALQIKTHRAGGLRCGVSDDRLYREEEEDACPEG 1359
            A++  +VVIRNLAWATHP LG     G I  R                 MA VV   T E + A      EGRMPSFR+IS LLYDYAVLRG+RPSLEAL  R+S   AS   P+A        + G + +  G              +PS  + +                                      F+ MH  LDR PVW L+GLA +  R+     ++ YR  G                                                                          SGG       R                +R G    I   + RLD+L ALP+VYE FTWQ YFFDSALRVP  L P GYALE ++ASELL ++L GG   G    KASPERR  +L+EL LQI+THR+GGLRCG   +      +    PEG
Sbjct:  472 AESRPDVVIRNLAWATHPVLGLGGS-GAIVARKRDTTVXXXXXXXXXXXMAAVVAGRTAERKGAVGSGAGEGRMPSFREISGLLYDYAVLRGMRPSLEALAMRSSFAAASVSSPAATAPGTPCVAGGGDTSGGGLSSSRSRPQSPYPPSPSGGSGEGQRGNPPTAGDGGFQGRREAGLLLLRGGGLRPSLPRLAFDAMHLLLDRSPVWALQGLAALAERVGVFGGSSGYRHGG--------------------------------------------------------------------------SGGXXXXXXXR----------------RRTG----ITVTLRRLDKLAALPAVYETFTWQEYFFDSALRVPEGLKPAGYALETALASELLQRSLPGGAEEG---AKASPERRQSILRELGLQIRTHRSGGLRCGGRGNDTGTSLQVGGAPEG 831
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig84139.16025.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
A0A6H5KHQ5_9PHAE2.450e-4533.70FAD-binding PCMH-type domain-containing protein n=... [more]
D7FUE9_ECTSI3.180e-4332.97Alkylglycerone-phosphate synthase n=1 Tax=Ectocarp... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig84139contigH-elongata_contig84139:326..2603 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score171.0
Seed ortholog evalue1.3e-39
Seed eggNOG ortholog2880.D7FUE9
Preferred nameAGPS
KEGG rclassRC00004,RC00020,RC02886
KEGG koko:K00803,ko:K13356
KEGG ReactionR04311,R09470
KEGG Pathwayko00073,ko00565,ko01100,ko04146,ko04212,map00073,map00565,map01100,map04146,map04212
GOsGO:0000166,GO:0003674,GO:0003824,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005730,GO:0005737,GO:0005739,GO:0005777,GO:0005778,GO:0005782,GO:0005829,GO:0006605,GO:0006625,GO:0006629,GO:0006662,GO:0006810,GO:0006886,GO:0006996,GO:0007031,GO:0008104,GO:0008150,GO:0008152,GO:0008609,GO:0008610,GO:0008611,GO:0009058,GO:0009987,GO:0015031,GO:0015833,GO:0016020,GO:0016043,GO:0016740,GO:0016765,GO:0018904,GO:0031090,GO:0031903,GO:0031907,GO:0031974,GO:0031981,GO:0033036,GO:0033365,GO:0034613,GO:0036094,GO:0042579,GO:0042886,GO:0043167,GO:0043168,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043574,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044281,GO:0044422,GO:0044424,GO:0044428,GO:0044438,GO:0044439,GO:0044444,GO:0044446,GO:0044464,GO:0045184,GO:0046485,GO:0046504,GO:0046907,GO:0048037,GO:0050660,GO:0050662,GO:0051179,GO:0051234,GO:0051641,GO:0051649,GO:0070013,GO:0070727,GO:0071702,GO:0071704,GO:0071705,GO:0071840,GO:0071949,GO:0072594,GO:0072662,GO:0072663,GO:0097159,GO:0097384,GO:0098588,GO:0098805,GO:1901265,GO:1901363,GO:1901503,GO:1901576
EggNOG free text desc.alkylglycerone-phosphate synthase activity
EggNOG OGsCOG0277@1,COG3320@1,KOG1221@2759,KOG1233@2759
EC1.2.1.84,2.5.1.26
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Hectar predicted targeting categoryother localisation
Exons2
Model size1422
Cds size1419
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931246.403172-CDS-H-elongata_contig84139:325..15091622931246.403172-CDS-H-elongata_contig84139:325..1509Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig84139 326..1509 +
1691679758.080267-CDS-H-elongata_contig84139:325..15091691679758.080267-CDS-H-elongata_contig84139:325..1509Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig84139 326..1509 +
1622931246.4172807-CDS-H-elongata_contig84139:2367..26021622931246.4172807-CDS-H-elongata_contig84139:2367..2602Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig84139 2368..2602 +
1691679758.0922627-CDS-H-elongata_contig84139:2367..26021691679758.0922627-CDS-H-elongata_contig84139:2367..2602Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig84139 2368..2602 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig84139.16025.1prot_H-elongata_contig84139.16025.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig84139 326..2602 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig84139.16025.1

>prot_H-elongata_contig84139.16025.1 ID=prot_H-elongata_contig84139.16025.1|Name=mRNA_H-elongata_contig84139.16025.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=473bp
RIAPGTNSGDSTGVHRGQGVGNVADPRTPPHAEALDISPQTLTRPGGKDC
YDADADSEVVIRNLAWATHPALGRRCPVGGISTRHSQAEYYGSVGEGMAL
VVTTPEEEEAQREEEGRMPSFRDISSLLYDYAVLRGLRPSLEALTSRTSI
LLASKPSADRVNLRGFSAGCERTWDGDAAPSESTSKSSVFETMHFFLDRL
PVWLLRGLAIIVRRLPRSRRTNCYRTEGLSRPPPAASCNRLAEGGEGLER
FSNMAVAETDQEAREKCLAGDGEGTVMIASGSSVAVEDEVIADMDADAGA
NNSGGVDRLVEARGRALPRICEPGTPQSTKRCGPGVDIIALVERLDRLTA
LPSVYEPFTWQRYFFDSALRVPRALTPEGYALEASIASELLIQNLEGGLR
CGDANTKASPERRDEVLQELALQIKTHRAGGLRCGVSDDRLYREEEEDAC
PEGMGTGGALIEKDGGGEGRFGF
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mRNA from alignment at H-elongata_contig84139:326..2603+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig84139.16025.1 ID=mRNA_H-elongata_contig84139.16025.1|Name=mRNA_H-elongata_contig84139.16025.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2278bp|location=Sequence derived from alignment at H-elongata_contig84139:326..2603+ (Himanthalia elongata Himel1 dioecious)
CGCATTGCACCAGGCACCAATAGCGGTGATAGTACGGGCGTCCATCGTGG
TCAGGGTGTGGGCAACGTCGCCGATCCTCGAACTCCGCCGCACGCCGAAG
CGTTAGACATATCGCCGCAAACTCTCACCCGGCCAGGTGGTAAAGATTGT
TACGATGCTGATGCGGACTCTGAAGTGGTCATAAGGAATCTGGCGTGGGC
AACGCATCCTGCCCTTGGGCGGAGATGTCCCGTAGGGGGCATTTCGACGC
GTCACAGCCAAGCAGAATATTATGGTTCCGTCGGCGAAGGTATGGCTTTA
GTCGTAACAACGCCGGAAGAAGAGGAGGCGCAGCGAGAAGAAGAGGGTCG
AATGCCGTCATTCCGAGATATTTCTAGTCTCCTGTACGACTATGCGGTCC
TCCGCGGACTGCGGCCGTCCCTCGAGGCCCTGACTTCCAGAACCTCGATT
CTCTTGGCTTCGAAGCCATCCGCCGATCGCGTCAACCTTCGCGGTTTTTC
TGCCGGCTGCGAACGAACTTGGGATGGTGATGCCGCGCCTTCGGAGTCCA
CCTCCAAGTCGTCGGTGTTCGAGACGATGCATTTTTTCCTCGACAGGCTC
CCCGTTTGGCTCCTCCGAGGGCTCGCGATTATCGTGCGACGGCTGCCGCG
CTCGCGTCGAACGAACTGTTACCGCACCGAGGGACTCAGCCGCCCACCGC
CAGCTGCGTCTTGCAATCGCCTTGCCGAAGGCGGGGAAGGCTTGGAGAGA
TTCTCGAACATGGCCGTGGCGGAGACTGACCAAGAAGCGAGGGAAAAATG
CCTGGCGGGCGATGGAGAAGGCACGGTAATGATCGCCTCGGGATCTTCAG
TTGCGGTGGAAGATGAAGTGATCGCGGACATGGATGCAGACGCAGGCGCA
AATAACTCGGGTGGCGTAGACAGGCTCGTGGAGGCTAGGGGAAGAGCACT
CCCAAGGATTTGTGAACCCGGAACGCCGCAATCGACGAAGCGCTGCGGTC
CGGGCGTTGATATCATTGCTTTGGTGGAGCGTCTCGACCGGCTCACGGCA
TTGCCCTCTGTGTATGAGCCCTTCACCTGGCAGCGGTACTTCTTCGACTC
CGCGCTGCGCGTACCGCGCGCGCTGACGCCGGAAGGCTACGCTCTTGAGG
CCTCGATTGCTTCGGAGCTATTGATCCAGAACCTGTGAGCCGATCGACAG
TAGCGTAACCGTTGCCCCTCGGCGTCGGTGCATTGCTCTTTGGTTTTATT
GTAACCATCTCTTTTGGTGCATTAGTCTAATCGTCTTCAGCGGGGCTTGG
TTGGGAGTTAATGTCATGCAAGAGGAGACTGCTACGGTTCAACGCAGAGC
GATTCGCGTTGTGTAGCGAGTCGTTTCGCGGGGAGTCACGCACATAGACG
ATAGCGTGTCGTGTTCGCCTTGGATCGTAGCACCTCTCGGTTTCCCTTGT
AAATCATGTACCATGTAGCGCATGTATCATATAGCATCCTCCTCTCTGCG
CTCTGTGTAGTCTGTCTGCAGATAAATGGCTGACGAAATAGTGATGCCGG
ACACGTAGATAATCCCATGTGTGTGCGTGTGTGTGTTTGTGATTAAGGAA
GGAACATGCTCGAGGGCCATCAAAATGGTATGCTAAAACAGTTCCTTTGC
ACCTTAACACAACTCATTATTAATAGCAGTTGGAGGGTGAGGTGACTAGC
CTTATTTGTACGTGCTGACGATATTTATGGGTATTTATGGGTATTTATGG
GTATTGTCAGCAATTGGATATACGACTAAGTGACTAGTGATTATAGTCGT
GACGGGGACAAAGATCGTTTTGGGGTGGGCCTTGCGAGTATCACACAATA
GGAAAATACTAAGCGTGTTCTAGATATTACTAGTTAGATGCTCTCAGAAT
TTCTACCCCAGCGCACTCGGTCTGTGTATAGTCTACCCCTCCTCTCCCCA
AATGTTCAGCCGCTATCGCCATCATTCATCATTCATCTACTGGATCCCAT
TTTTACGCGCGTTTTTTTATTTGTACGTTAACTTACTCCCAGAGAGGGCG
GCTTACGATGCGGGGACGCCAACACCAAGGCTAGTCCGGAGCGCCGAGAT
GAGGTTCTCCAGGAACTGGCGCTGCAGATAAAGACCCACCGAGCGGGCGG
CTTACGATGTGGAGTGAGCGACGATCGTCTCTATCGAGAAGAGGAGGAGG
ATGCTTGTCCAGAGGGGATGGGGACGGGGGGGGCTCTCATTGAAAAGGAT
GGAGGCGGAGAGGGTCGGTTTGGCTTCC
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Coding sequence (CDS) from alignment at H-elongata_contig84139:326..2603+

>mRNA_H-elongata_contig84139.16025.1 ID=mRNA_H-elongata_contig84139.16025.1|Name=mRNA_H-elongata_contig84139.16025.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=2838bp|location=Sequence derived from alignment at H-elongata_contig84139:326..2603+ (Himanthalia elongata Himel1 dioecious)
CGCATTGCACCAGGCACCAATAGCGGTGATAGTACGGGCGTCCATCGTGG
TCAGGGTGTGGGCAACGTCGCCGATCCTCGAACTCCGCCGCACGCCGAAG
CGTTAGACATATCGCCGCAAACTCTCACCCGGCCAGGTGGTAAAGATTGT
TACGATGCTGATGCGGACTCTGAAGTGGTCATAAGGAATCTGGCGTGGGC
AACGCATCCTGCCCTTGGGCGGAGATGTCCCGTAGGGGGCATTTCGACGC
GTCACAGCCAAGCAGAATATTATGGTTCCGTCGGCGAAGGTATGGCTTTA
GTCGTAACAACGCCGGAAGAAGAGGAGGCGCAGCGAGAAGAAGAGGGTCG
AATGCCGTCATTCCGAGATATTTCTAGTCTCCTGTACGACTATGCGGTCC
TCCGCGGACTGCGGCCGTCCCTCGAGGCCCTGACTTCCAGAACCTCGATT
CTCTTGGCTTCGAAGCCATCCGCCGATCGCGTCAACCTTCGCGGTTTTTC
TGCCGGCTGCGAACGAACTTGGGATGGTGATGCCGCGCCTTCGGAGTCCA
CCTCCAAGTCGTCGGTGTTCGAGACGATGCATTTTTTCCTCGACAGGCTC
CCCGTTTGGCTCCTCCGAGGGCTCGCGATTATCGTGCGACGGCTGCCGCG
CTCGCGTCGAACGAACTGTTACCGCACCGAGGGACTCAGCCGCCCACCGC
CAGCTGCGTCTTGCAATCGCCTTGCCGAAGGCGGGGAAGGCTTGGAGAGA
TTCTCGAACATGGCCGTGGCGGAGACTGACCAAGAAGCGAGGGAAAAATG
CCTGGCGGGCGATGGAGAAGGCACGGTAATGATCGCCTCGGGATCTTCAG
TTGCGGTGGAAGATGAAGTGATCGCGGACATGGATGCAGACGCAGGCGCA
AATAACTCGGGTGGCGTAGACAGGCTCGTGGAGGCTAGGGGAAGAGCACT
CCCAAGGATTTGTGAACCCGGAACGCCGCAATCGACGAAGCGCTGCGGTC
CGGGCGTTGATATCATTGCTTTGGTGGAGCGTCTCGACCGGCTCACGGCA
TTGCCCTCTGTGTATGAGCCCTTCACCTGGCAGCGGTACTTCTTCGACTC
CGCGCTGCGCGTACCGCGCGCGCTGACGCCGGAAGGCTACGCTCTTGAGG
CCTCGATTGCTTCGGAGCTATTGATCCAGAACCTCGCATTGCACCAGGCA
CCAATAGCGGTGATAGTACGGGCGTCCATCGTGGTCAGGGTGTGGGCAAC
GTCGCCGATCCTCGAACTCCGCCGCACGCCGAAGCGTTAGACATATCGCC
GCAAACTCTCACCCGGCCAGGTGGTAAAGATTGTTACGATGCTGATGCGG
ACTCTGAAGTGGTCATAAGGAATCTGGCGTGGGCAACGCATCCTGCCCTT
GGGCGGAGATGTCCCGTAGGGGGCATTTCGACGCGTCACAGCCAAGCAGA
ATATTATGGTTCCGTCGGCGAAGGTATGGCTTTAGTCGTAACAACGCCGG
AAGAAGAGGAGGCGCAGCGAGAAGAAGAGGGTCGAATGCCGTCATTCCGA
GATATTTCTAGTCTCCTGTACGACTATGCGGTCCTCCGCGGACTGCGGCC
GTCCCTCGAGGCCCTGACTTCCAGAACCTCGATTCTCTTGGCTTCGAAGC
CATCCGCCGATCGCGTCAACCTTCGCGGTTTTTCTGCCGGCTGCGAACGA
ACTTGGGATGGTGATGCCGCGCCTTCGGAGTCCACCTCCAAGTCGTCGGT
GTTCGAGACGATGCATTTTTTCCTCGACAGGCTCCCCGTTTGGCTCCTCC
GAGGGCTCGCGATTATCGTGCGACGGCTGCCGCGCTCGCGTCGAACGAAC
TGTTACCGCACCGAGGGACTCAGCCGCCCACCGCCAGCTGCGTCTTGCAA
TCGCCTTGCCGAAGGCGGGGAAGGCTTGGAGAGATTCTCGAACATGGCCG
TGGCGGAGACTGACCAAGAAGCGAGGGAAAAATGCCTGGCGGGCGATGGA
GAAGGCACGGTAATGATCGCCTCGGGATCTTCAGTTGCGGTGGAAGATGA
AGTGATCGCGGACATGGATGCAGACGCAGGCGCAAATAACTCGGGTGGCG
TAGACAGGCTCGTGGAGGCTAGGGGAAGAGCACTCCCAAGGATTTGTGAA
CCCGGAACGCCGCAATCGACGAAGCGCTGCGGTCCGGGCGTTGATATCAT
TGCTTTGGTGGAGCGTCTCGACCGGCTCACGGCATTGCCCTCTGTGTATG
AGCCCTTCACCTGGCAGCGGTACTTCTTCGACTCCGCGCTGCGCGTACCG
CGCGCGCTGACGCCGGAAGGCTACGCTCTTGAGGCCTCGATTGCTTCGGA
GCTATTGATCCAGAACCTAGAGGGCGGCTTACGATGCGGGGACGCCAACA
CCAAGGCTAGTCCGGAGCGCCGAGATGAGGTTCTCCAGGAACTGGCGCTG
CAGATAAAGACCCACCGAGCGGGCGGCTTACGATGTGGAGTGAGCGACGA
TCGTCTCTATCGAGAAGAGGAGGAGGATGCTTGTCCAGAGGGGATGGGGA
CGGGGGGGGCTCTCATTGAAAAGGATGGAGGCGGAGAGGGTCGGTTTGGC
TTCAGAGGGCGGCTTACGATGCGGGGACGCCAACACCAAGGCTAGTCCGG
AGCGCCGAGATGAGGTTCTCCAGGAACTGGCGCTGCAGATAAAGACCCAC
CGAGCGGGCGGCTTACGATGTGGAGTGAGCGACGATCGTCTCTATCGAGA
AGAGGAGGAGGATGCTTGTCCAGAGGGGATGGGGACGGGGGGGGCTCTCA
TTGAAAAGGATGGAGGCGGAGAGGGTCGGTTTGGCTTC
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