mRNA_H-elongata_contig57911.13265.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig57911.13265.1
Unique NamemRNA_H-elongata_contig57911.13265.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: D7G5W8_ECTSI (Putative homoaconitate hydratase, c-terminal part n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5W8_ECTSI)

HSP 1 Score: 155 bits (392), Expect = 3.710e-45
Identity = 75/92 (81.52%), Postives = 84/92 (91.30%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLKV 276
            AKY+SK+ YD++ LEPVVAKPHSPDN+ALAK+CR  KIDRVYIGSCTGGKTEDFAAAARILQN+K    VRVPTFLVPATQKVYND+QTLK+
Sbjct:   83 AKYFSKKRYDMSNLEPVVAKPHSPDNKALAKDCRGTKIDRVYIGSCTGGKTEDFAAAARILQNQK----VRVPTFLVPATQKVYNDIQTLKI 170
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BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: A0A6H5KHV3_9PHAE (3-isopropylmalate dehydratase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KHV3_9PHAE)

HSP 1 Score: 159 bits (401), Expect = 2.690e-44
Identity = 76/92 (82.61%), Postives = 86/92 (93.48%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLKV 276
            AKY+SK++YD++ LEPVVAKPHSPDN+ALAK+CRD KIDRVYIGSCTGGKTEDFAAAARILQN+K    VRVPTFLVPATQKVYND+QTLK+
Sbjct:  340 AKYFSKKTYDMSNLEPVVAKPHSPDNKALAKDCRDTKIDRVYIGSCTGGKTEDFAAAARILQNQK----VRVPTFLVPATQKVYNDIQTLKI 427
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BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: X2KY86_SCYLO (3-isopropylmalate dehydratase n=1 Tax=Scytosiphon lomentaria TaxID=27967 RepID=X2KY86_SCYLO)

HSP 1 Score: 154 bits (390), Expect = 1.370e-42
Identity = 74/92 (80.43%), Postives = 83/92 (90.22%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLKV 276
            AKY+SKQ YD+  LEPVVAKPHSPDN+ALA++CRD KIDRVYIGSCTGGKTEDFAAAARILQN+K    VRVPTFLVPATQKVYND+QT+ +
Sbjct:  359 AKYFSKQRYDVGNLEPVVAKPHSPDNKALARDCRDTKIDRVYIGSCTGGKTEDFAAAARILQNQK----VRVPTFLVPATQKVYNDIQTMTI 446
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BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: A0A835YVF1_9STRA (3-isopropylmalate dehydratase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YVF1_9STRA)

HSP 1 Score: 144 bits (364), Expect = 2.770e-39
Identity = 68/92 (73.91%), Postives = 81/92 (88.04%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLKV 276
            A Y++ ++YD+++LEPVVA PHSPDN+ALAK+CRD KIDRVYIGSCTGGKTEDFAAAAR+L  +K    VRVPTFLVPATQKVYND+Q LK+
Sbjct:  288 ATYFADKTYDMSQLEPVVAMPHSPDNKALAKDCRDTKIDRVYIGSCTGGKTEDFAAAARVLDGQK----VRVPTFLVPATQKVYNDIQNLKI 375
[Close]
BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: W7TZ20_9STRA (3-isopropylmalate dehydratase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TZ20_9STRA)

HSP 1 Score: 142 bits (358), Expect = 3.570e-38
Identity = 69/90 (76.67%), Postives = 78/90 (86.67%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTL 270
            A Y++K +YDL+ LEPVVAKPHSPDNRALA+ECRDIKIDRVYIGSCTGGKTEDF AAARILQ  K S    VPTFLVPAT++VY+DL +L
Sbjct:  322 ASYFAKYAYDLSSLEPVVAKPHSPDNRALARECRDIKIDRVYIGSCTGGKTEDFVAAARILQQNKVS----VPTFLVPATRRVYDDLYSL 407
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BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: A0A6P1AAH6_9CYAN (3-isopropylmalate dehydratase large subunit n=2 Tax=Oscillatoriales TaxID=1150 RepID=A0A6P1AAH6_9CYAN)

HSP 1 Score: 140 bits (352), Expect = 8.850e-38
Identity = 65/91 (71.43%), Postives = 81/91 (89.01%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLK 273
            AK+YS ++YD+++LEPVVAKPHSPDNRALA+ECRD+KIDRVYIGSCTGGKT DF  AAR+L+ +K    V+VPT++VPATQKVY+DL T+K
Sbjct:  267 AKFYSDRTYDVSQLEPVVAKPHSPDNRALARECRDVKIDRVYIGSCTGGKTSDFLNAARVLKGRK----VKVPTYIVPATQKVYDDLFTVK 353
[Close]
BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: K9TYF3_CHRTP (Homoaconitate hydratase family protein n=26 Tax=Cyanobacteria TaxID=1117 RepID=K9TYF3_CHRTP)

HSP 1 Score: 137 bits (346), Expect = 6.650e-37
Identity = 66/91 (72.53%), Postives = 79/91 (86.81%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLK 273
            AK+YS + YD+++LEPVVA+PHSPDNRALA+EC D+KIDRVYIGSCTGGKTEDF  AA IL+ ++    V+VPT+LVPATQKVYNDL TLK
Sbjct:  267 AKFYSDRRYDVSQLEPVVAQPHSPDNRALARECSDVKIDRVYIGSCTGGKTEDFIHAAHILKGQQ----VKVPTYLVPATQKVYNDLFTLK 353
[Close]
BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: A0A2T1E8G0_9CHRO (3-isopropylmalate dehydratase n=2 Tax=Cyanobacteria TaxID=1117 RepID=A0A2T1E8G0_9CHRO)

HSP 1 Score: 136 bits (342), Expect = 2.590e-36
Identity = 66/91 (72.53%), Postives = 77/91 (84.62%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLK 273
            AK+YS  SYD+++LEPVVAKPHSPDNRALA+EC D+KIDRVYIGSCTGGKT DF  AA+IL+ +K    V+VPT+LVPATQKVY DL T K
Sbjct:  267 AKFYSSHSYDVSQLEPVVAKPHSPDNRALARECSDVKIDRVYIGSCTGGKTTDFINAAQILKGQK----VKVPTYLVPATQKVYEDLFTQK 353
[Close]
BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: A0A1U7IRT4_9CYAN (3-isopropylmalate dehydratase n=6 Tax=Cyanobacteria TaxID=1117 RepID=A0A1U7IRT4_9CYAN)

HSP 1 Score: 135 bits (341), Expect = 3.620e-36
Identity = 66/91 (72.53%), Postives = 76/91 (83.52%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLK 273
            AK+YS+  YD++KLEPVVAKPHSPDNRALAKEC D+KI+RVYIGSCTGGKT DF  AARI++  K    V+VPT+LVPATQKVY DL T K
Sbjct:  267 AKFYSQHHYDVSKLEPVVAKPHSPDNRALAKECSDVKINRVYIGSCTGGKTSDFLNAARIIKGHK----VKVPTYLVPATQKVYEDLFTQK 353
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BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Match: A0A446W303_TRITD (Uncharacterized protein n=2 Tax=Poaceae TaxID=4479 RepID=A0A446W303_TRITD)

HSP 1 Score: 131 bits (329), Expect = 5.320e-36
Identity = 62/92 (67.39%), Postives = 77/92 (83.70%), Query Frame = 1 
Query:    1 AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGKTEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLKV 276
            A++YS   +D++KLEPVVAKPHSPDNRALA+EC+D+KIDRVYIGSCTGGKTEDF AAA++     +   V+VPTFLVPATQKV+ D+ +L V
Sbjct:   76 ARFYSDYRFDVSKLEPVVAKPHSPDNRALARECKDVKIDRVYIGSCTGGKTEDFIAAAKVFL--ASGQKVKVPTFLVPATQKVWMDVYSLPV 165
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig57911.13265.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G5W8_ECTSI3.710e-4581.52Putative homoaconitate hydratase, c-terminal part ... [more]
A0A6H5KHV3_9PHAE2.690e-4482.613-isopropylmalate dehydratase n=1 Tax=Ectocarpus s... [more]
X2KY86_SCYLO1.370e-4280.433-isopropylmalate dehydratase n=1 Tax=Scytosiphon ... [more]
A0A835YVF1_9STRA2.770e-3973.913-isopropylmalate dehydratase n=1 Tax=Tribonema mi... [more]
W7TZ20_9STRA3.570e-3876.673-isopropylmalate dehydratase n=2 Tax=Monodopsidac... [more]
A0A6P1AAH6_9CYAN8.850e-3871.433-isopropylmalate dehydratase large subunit n=2 Ta... [more]
K9TYF3_CHRTP6.650e-3772.53Homoaconitate hydratase family protein n=26 Tax=Cy... [more]
A0A2T1E8G0_9CHRO2.590e-3672.533-isopropylmalate dehydratase n=2 Tax=Cyanobacteri... [more]
A0A1U7IRT4_9CYAN3.620e-3672.533-isopropylmalate dehydratase n=6 Tax=Cyanobacteri... [more]
A0A446W303_TRITD5.320e-3667.39Uncharacterized protein n=2 Tax=Poaceae TaxID=4479... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig57911contigH-elongata_contig57911:761..3399 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score153.3
Seed ortholog evalue5.5e-35
Seed eggNOG ortholog2880.D7G5W8
Preferred nameLEU1
KEGG rclassRC00497,RC00976,RC00977,RC01041,RC01046,RC03072
KEGG koko:K00749,ko:K01702,ko:K01703
KEGG ReactionR03896,R03898,R03968,R04001,R08620,R08624,R08628,R08634,R08641,R08645,R10170
KEGG Pathwayko00290,ko00660,ko00966,ko01100,ko01110,ko01210,ko01230,map00290,map00660,map00966,map01100,map01110,map01210,map01230
KEGG ModuleM00432,M00535
GOsGO:0003674,GO:0003824,GO:0003861,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006082,GO:0006520,GO:0006551,GO:0006790,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009081,GO:0009082,GO:0009098,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009987,GO:0010035,GO:0010038,GO:0010494,GO:0016053,GO:0016143,GO:0016144,GO:0016829,GO:0016835,GO:0016836,GO:0016853,GO:0016866,GO:0019748,GO:0019752,GO:0019757,GO:0019758,GO:0019760,GO:0019761,GO:0032991,GO:0035770,GO:0036464,GO:0042221,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044272,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0044550,GO:0046394,GO:0046686,GO:0050486,GO:0050896,GO:0071704,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607,GO:1901657,GO:1901659,GO:1990904
EggNOG free text desc.3-isopropylmalate dehydratase activity
EggNOG OGsCOG0065@1,KOG0454@2759
EC2.4.1.183,4.2.1.33,4.2.1.35
COG Functional cat.E
CAZyGT5
BiGG ReactioniMM904.YGL009C,iND750.YGL009C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01601,ko00000,ko00001,ko00002,ko01000,ko01003
Hectar predicted targeting categoryother localisation
Exons2
Model size276
Cds size276
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930939.9468114-CDS-H-elongata_contig57911:760..9671622930939.9468114-CDS-H-elongata_contig57911:760..967Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57911 761..967 +
1691679633.1845326-CDS-H-elongata_contig57911:760..9671691679633.1845326-CDS-H-elongata_contig57911:760..967Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57911 761..967 +
1622930939.9671495-CDS-H-elongata_contig57911:3330..33991622930939.9671495-CDS-H-elongata_contig57911:3330..3399Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57911 3331..3399 +
1691679633.1966648-CDS-H-elongata_contig57911:3330..33991691679633.1966648-CDS-H-elongata_contig57911:3330..3399Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57911 3331..3399 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig57911.13265.1prot_H-elongata_contig57911.13265.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig57911 761..3399 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig57911.13265.1

>prot_H-elongata_contig57911.13265.1 ID=prot_H-elongata_contig57911.13265.1|Name=mRNA_H-elongata_contig57911.13265.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=92bp
AKYYSKQSYDLNKLEPVVAKPHSPDNRALAKECRDIKIDRVYIGSCTGGK
TEDFAAAARILQNKKASSTVRVPTFLVPATQKVYNDLQTLKV
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mRNA from alignment at H-elongata_contig57911:761..3399+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig57911.13265.1 ID=mRNA_H-elongata_contig57911.13265.1|Name=mRNA_H-elongata_contig57911.13265.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2639bp|location=Sequence derived from alignment at H-elongata_contig57911:761..3399+ (Himanthalia elongata Himel1 dioecious)
GCGAAGTACTACTCTAAGCAGAGCTACGACCTGAACAAGCTCGAGCCTGT
CGTGGCGAAGCCTCACTCTCCCGATAACAGGGCCCTGGCAAAGGAATGCC
GTGACATTAAGATCGACCGCGTCTACATTGGCTCCTGCACGGGGGGCAAG
ACGGAGGATTTTGCGGCGGCTGCCCGCATCCTCCAAAACAAGAAGGCTAG
TAGTACTGTAATGTGAACCATCCAAGGATAAAGGGGGTCCGTGGGGGGGG
TGGCTTAAGCGTTTCCGGTTCGGGAAAAGGGGACGAAACGTACCCTACTT
CTCCTCAATCGGGGATACGATAATTGGATCCTCGAGAGGGTATGATATTA
TTTTTCCCTCCCTAGACTGGGGACACGTAAGCAATTTTCCCGTCCTTATC
GTCGGACGTGTAATCGGCACGCACAGCCTCCGAGATAATTATGAAATATC
TCTGAGATCACTCTGAAATAATTACTCTGAGATAACAATCCTAGTAAGCG
TGGTGGGGTTGGGCATGAATAGACGCCGAGAGGCTGCGAAAAAATAAAAG
AGGGTCCGGCCCACGATTTTATCGCTTAACTTGTGGTCTGTGGCTGAAGC
AAAGACCTTCTCGACACCATTCGGTTGACACCATTCGGTTGAAGGGCATT
TGGCTTACAAACATGATACACAGTACAGTAGTACACAGTACTGTATCTAC
CGTGTACCGTGTGTGATAACTCTCAAAGCCAGCGTCTTGTTTAGGTAAAT
TTTTGCTGTAGTCATTTTTGTTAGCCTACACACATACGTCAAGGACTTTT
ACTAATACTAAGACTATGACTAGCTGACAAGGCCGGATTGCTGAATTGTG
CAGTTATGTGCAATTTTGTAATTACGCACACACGCACACAAGTATAGAGT
ATGCCCTTACCCCCTACCCTCAATTTTGAAGTTTCCAATTCGATACTGTA
CACGACCACGTGATCATTTTCATCCAGATCGAAAATGGTCCCTGGCTTTA
AGAGGGTATTTTATCCCATGCGCGCCTATGGTTCCACTGTTCAAATCCAC
ACGACATGACAATCCTCTAGTATCAATTGTAGACAGTTACATAGTATCAC
GTTATCCAACAGTCCCGAGTTCAAATCTCGGTAAAAAAAAATGGTTGTGT
AGGTGTACAGGACTGTACAGCCTCGGAAAAGCATGAGGGTGGTTATCCAA
CAGTCCCGAGTTTAAATCTCGGTTAAAAAAAGGGTCGTGTAGGTGTACAG
TCTCGGAAATGCGTGAGTGTGGTAGTACCGTGCACGGGACTGTACTGCCC
GAGCAATCAAAGTTCAACCATTGACTGTCGAGGGGTAGGAGCAGCATCGA
GGTTAGATCGTGGTGAAAACGAAAAGGCGCACAGTAGGCATTAAGCAAAC
GGGGAAATATATATGAGGTGGAATGGGCGATGTTGCCGTAGAGGTAGGAT
TGGTACAGTAACCTAGCACGTCGAATGGTATATATATACCACGTTAGGAC
TAAAAGTGTTATTAAATTAATATAGTAACGCCGCACTGTAATTAATTATT
GATGAGATCGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
CCCTTTTTCCCCCCCCCCCACCCAAAACAAAAAAATACAACTTTTTTTTC
TTTTTTTTNNNNNNNNNNCCCTAAAAATTTATTATACAGCGCCCCCCCCC
CCCCCCCCCCCACACACACACGCCTTCACCTTGTTTTCCCACACACCGGA
AGGAATATAGAATTAACGCGTTTTTTTCTTCCTGGGGAGAAAACTTCCCC
TTCTTTCTTTCATTAATATATACATACTTGGTGTTATATATTAAGTTGTT
TTTTGTGTTTTTTTAACTTCTCTAAGCGATGGGGGCTAAAATATTTTTTC
GGAGTGTTCTCGATCGACAAGATATATCCAACGGTATACTGTGTATTAGG
GAAGTATTTTGTCAGATCGCCCCGGGGCCAAGAAACATAGAGAGGAGGGG
AGGAGGAGGCGGGGGGGCGGGGGGGGCGTTAATTCCAACCGTTTGCAGCG
ATTCAGTATGGTGAAAGGATCGAAAATCGCCCATGAAAATATTTCTGCAC
ACACCCATCCACCTTCCATTTTTTTAGTGCAATATTTTACCATAATATCA
GCGTAGGGTAGTCTACACGATTATCAGCATGTGTCGATAATTTAATTTAA
AATAGTTCACGCCATTCTCTTTTATCCTACGCGACCGCTTATATTAACCG
CAAGCAAGCAAGCAACGTCTATTGTTGAAGAAAAAAATGCACGGCCGAGG
TAAACGGTTTAAGGTTAATTATCGGTACACAGTTCATTCACAGTTCTTGT
TTGAGGACAGACGGGGTTGCTTTTCATGCTGCCTTGTCTCCGGGTCGGTA
AAAATCGAACGGGGATATCTATCCACCATCCCCTGGGGATGATGGATAGA
GGCCTAAGAGGCATGGTGTGCTGTCTTGTCTATTGAACGTTAAATATATA
ATTGACCGTATCGTACATCGTGCATCGTGCTGCCTTACCCTTCCCTCTAA
ATGTTGTTTTGGCGAGGCAGGTTCGGGTGCCGACCTTCCTCGTGCCGGCG
ACGCAAAAGGTCTACAACGACCTACAGACGCTCAAGGTA
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Coding sequence (CDS) from alignment at H-elongata_contig57911:761..3399+

>mRNA_H-elongata_contig57911.13265.1 ID=mRNA_H-elongata_contig57911.13265.1|Name=mRNA_H-elongata_contig57911.13265.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=552bp|location=Sequence derived from alignment at H-elongata_contig57911:761..3399+ (Himanthalia elongata Himel1 dioecious)
GCGAAGTACTACTCTAAGCAGAGCTACGACCTGAACAAGCTCGAGCCTGT
CGTGGCGAAGCCTCACTCTCCCGATAACAGGGCCCTGGCAAAGGAATGCC
GTGACATTAAGATCGACCGCGTCTACATTGGCTCCTGCACGGGGGGCAAG
ACGGAGGATTTTGCGGCGGCTGCCCGCATCCTCCAAAACAAGAAGGCTAG
TAGTACTGCGAAGTACTACTCTAAGCAGAGCTACGACCTGAACAAGCTCG
AGCCTGTCGTGGCGAAGCCTCACTCTCCCGATAACAGGGCCCTGGCAAAG
GAATGCCGTGACATTAAGATCGACCGCGTCTACATTGGCTCCTGCACGGG
GGGCAAGACGGAGGATTTTGCGGCGGCTGCCCGCATCCTCCAAAACAAGA
AGGCTAGTAGTACTGTTCGGGTGCCGACCTTCCTCGTGCCGGCGACGCAA
AAGGTCTACAACGACCTACAGACGCTCAAGGTAGTTCGGGTGCCGACCTT
CCTCGTGCCGGCGACGCAAAAGGTCTACAACGACCTACAGACGCTCAAGG
TA
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