mRNA_H-elongata_contig48140.11955.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig48140.11955.1
Unique NamemRNA_H-elongata_contig48140.11955.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: A0A6H5L446_9PHAE (UDENN domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5L446_9PHAE)

HSP 1 Score: 181 bits (458), Expect = 3.370e-50
Identity = 99/133 (74.44%), Postives = 111/133 (83.46%), Query Frame = 1 
Query:    1 QVSIHCPAFLRAPLEVTSAPLEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVGVQAPFEAPLVNPLGYALDSVVVLDLDARPAS 399
            +VS++CPAFLRAPLE+T   LE W+TAVLF  V E TILRA+D+LLLEKTLVVCGRDIG+VS+ ATALLSLLDPFKWEGVFVPVVPL +MDVLDSPVPALVGVQAP     ++PL  A D V VLDLDA  AS
Sbjct:  653 KVSLYCPAFLRAPLELTPPSLELWSTAVLFGCVSERTILRALDLLLLEKTLVVCGRDIGIVSMAATALLSLLDPFKWEGVFVPVVPLALMDVLDSPVPALVGVQAP-----LDPLSSAADGVAVLDLDANNAS 780
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: A0A4D9CNF6_9STRA (UDENN domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9CNF6_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 8.160e-21
Identity = 51/106 (48.11%), Postives = 73/106 (68.87%), Query Frame = 1 
Query:   40 LEVTSAPLEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVGVQAPFEAPLVNPLGYAL 357
            L++   PL+ W  AVLF+ V    I++ V++LLLE++LVV G D G+VS  +TA +SLL PFKW+GVF+P++P  + D+L SPVP ++GVQ PF+     P   AL
Sbjct:  500 LDLPLVPLQDWTVAVLFSHVSVDVIIKCVNLLLLEQSLVVIGEDQGLVSAISTAFVSLLQPFKWDGVFIPLLPTALSDILQSPVPFVLGVQPPFDPTRSTPHAAAL 605
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: A0A482S8K5_9ARCH (DENN domain-containing protein (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S8K5_9ARCH)

HSP 1 Score: 75.1 bits (183), Expect = 2.990e-13
Identity = 42/106 (39.62%), Postives = 66/106 (62.26%), Query Frame = 1 
Query:    4 VSIHCPAFL-RAPLEVTSAPLEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVGVQAP 318
            V++  P+++    +  T A ++ WA AVL   +P   I+    +LLLEK+L++ G D  +VSI  T  L LL P+KW G+FVP++P    +VL++PVP +VG+  P
Sbjct:   47 VNLSFPSYMDNLVVSKTQATIDMWAVAVLTYFIPTDVIVHIHLMLLLEKSLIILGSDAALVSILCTGFLHLLAPYKWTGIFVPLLPPLAQEVLEAPVPFIVGMYTP 152
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: A0A7S3GU22_9STRA (Hypothetical protein (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GU22_9STRA)

HSP 1 Score: 68.6 bits (166), Expect = 1.100e-11
Identity = 36/102 (35.29%), Postives = 63/102 (61.76%), Query Frame = 1 
Query:   10 IHCPAFL---RAPLEVTSAPLEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVG 306
            I CP ++   +  L+      E W  AVLF  +    I + +++LL+EK+L++ G++ G+V+    A+++L+ PF WEG+FVP+VP    ++  +PVP +VG
Sbjct:   14 IRCPGYIEDFKVDLKALLTTKE-WTCAVLFTHLSSQIIFKIINLLLMEKSLIIHGKNAGIVTTITMAVINLISPFLWEGIFVPLVPDNARELFGAPVPLIVG 114
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: A0A7S3GQI8_9STRA (Hypothetical protein n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GQI8_9STRA)

HSP 1 Score: 70.5 bits (171), Expect = 1.630e-11
Identity = 36/85 (42.35%), Postives = 55/85 (64.71%), Query Frame = 1 
Query:   61 LEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVGVQA 315
            L+ W  A+L A +P   I++ +  LL E++L+V G D GMV+   +A   LL PF WEG+ VP++P+   + LD+PVP ++GV A
Sbjct:  707 LKEWTVAILIALLPSELIVKILSQLLQEQSLLVYGSDAGMVTAVVSAFTLLLQPFSWEGIHVPLLPMAAYEALDAPVPFVIGVVA 791
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: A0A6J1JNC5_CUCMA (uncharacterized protein LOC111488447 isoform X1 n=9 Tax=Cucurbita TaxID=3660 RepID=A0A6J1JNC5_CUCMA)

HSP 1 Score: 69.3 bits (168), Expect = 4.040e-11
Identity = 35/82 (42.68%), Postives = 58/82 (70.73%), Query Frame = 1 
Query:   61 LEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVG 306
            L  W TA L  ++   T+L+ V V+LLEK ++V   ++G++S T  +L+ ++ PF+W+ +F+PV+P KM D+LD+PVP +VG
Sbjct:  563 LSIWTTATLCRALSLETVLQLVAVILLEKQVIVVCPNLGLLSATVLSLVPMICPFQWQSLFLPVLPGKMFDLLDAPVPFIVG 644
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: A0A484NLS7_9ASTE (UDENN domain-containing protein n=3 Tax=Cuscuta sect. Cleistogrammica TaxID=1824901 RepID=A0A484NLS7_9ASTE)

HSP 1 Score: 68.2 bits (165), Expect = 1.020e-10
Identity = 34/87 (39.08%), Postives = 60/87 (68.97%), Query Frame = 1 
Query:   52 SAPLEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVGVQ 312
            +A L  W TA    ++   TIL  +  +LLEK +V+   ++G++S    +L+ ++ PF+W+ +F+P++PLKM+D+LD+PVP +VG+Q
Sbjct:  590 AAALSVWTTAATCRALSLETILTMITGVLLEKQVVLICPNLGVLSALVLSLIPIIRPFQWQSLFLPILPLKMLDLLDAPVPFIVGLQ 676
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: UPI000901FF5B (uncharacterized protein LOC109173826 n=2 Tax=Ipomoea TaxID=4119 RepID=UPI000901FF5B)

HSP 1 Score: 67.4 bits (163), Expect = 1.900e-10
Identity = 34/84 (40.48%), Postives = 58/84 (69.05%), Query Frame = 1 
Query:   61 LEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVGVQ 312
            L  W TA +  ++   TIL  V  +LLEK +VV   ++G++S    +L+ ++ PF+W+ +F+P++P KM+D+LD+PVP +VG+Q
Sbjct:  578 LSVWTTATICRALSLETILALVTGVLLEKQVVVICPNLGILSALVLSLIPIIRPFQWQSLFLPILPRKMLDLLDAPVPFIVGLQ 661
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: UPI0008FEBA2E (uncharacterized protein LOC101261930 isoform X2 n=1 Tax=Solanum lycopersicum TaxID=4081 RepID=UPI0008FEBA2E)

HSP 1 Score: 67.4 bits (163), Expect = 1.900e-10
Identity = 34/84 (40.48%), Postives = 57/84 (67.86%), Query Frame = 1 
Query:   61 LEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVGVQ 312
            L  W TA +  S+   TIL  +  +LLEK +V+   ++G++S    +L+ ++ PF+W+ +F+P++P KMMD LD+PVP +VG+Q
Sbjct:  591 LSLWTTATICRSLSIETILALITGVLLEKQVVIVCPNLGVLSAVVLSLIPIIRPFQWQSLFLPILPGKMMDFLDAPVPFIVGLQ 674
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BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Match: A0A7S1YZ68_9STRA (Hypothetical protein (Fragment) n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1YZ68_9STRA)

HSP 1 Score: 67.0 bits (162), Expect = 2.660e-10
Identity = 50/132 (37.88%), Postives = 73/132 (55.30%), Query Frame = 1 
Query:    1 QVSIHCPAFLRAPLEVTSAPLEAWATAVLFASVPEATILRAVDVLLLEKTLVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPALVGVQAPF----EAPLVNPLGYALDSVVVLDLD 384
            QVSI  PA  R PL         ++   LFAS+    IL  +  LL E  + +C +   ++     AL SLL PF W+G+++P++P  M+DVLD+PVP LVG+ + +    +AP   P+G     VV +DLD
Sbjct:  503 QVSIVRPAANRLPLV-------GFSYHPLFASLSVGNILVVLGCLLKETRVAICSQHYALLGPVCEALTSLLFPFVWQGMYIPIMPYSMLDVLDAPVPFLVGLHSRYLKDVQAP-ARPIG-----VVFVDLD 621
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig48140.11955.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5L446_9PHAE3.370e-5074.44UDENN domain-containing protein n=2 Tax=Ectocarpus... [more]
A0A4D9CNF6_9STRA8.160e-2148.11UDENN domain-containing protein n=2 Tax=Monodopsid... [more]
A0A482S8K5_9ARCH2.990e-1339.62DENN domain-containing protein (Fragment) n=1 Tax=... [more]
A0A7S3GU22_9STRA1.100e-1135.29Hypothetical protein (Fragment) n=1 Tax=Spumella e... [more]
A0A7S3GQI8_9STRA1.630e-1142.35Hypothetical protein n=1 Tax=Spumella elongata Tax... [more]
A0A6J1JNC5_CUCMA4.040e-1142.68uncharacterized protein LOC111488447 isoform X1 n=... [more]
A0A484NLS7_9ASTE1.020e-1039.08UDENN domain-containing protein n=3 Tax=Cuscuta se... [more]
UPI000901FF5B1.900e-1040.48uncharacterized protein LOC109173826 n=2 Tax=Ipomo... [more]
UPI0008FEBA2E1.900e-1040.48uncharacterized protein LOC101261930 isoform X2 n=... [more]
A0A7S1YZ68_9STRA2.660e-1037.88Hypothetical protein (Fragment) n=1 Tax=Ditylum br... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig48140contigH-elongata_contig48140:190..2578 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score185.3
Seed ortholog evalue1.9e-44
Seed eggNOG ortholog2880.D7FZZ2
KEGG koko:K20161
EggNOG free text desc.Rab guanyl-nucleotide exchange factor activity
EggNOG OGsKOG3569@1,KOG3569@2759
COG Functional cat.A
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionDENN domain
Ec32 orthologEc-22_003540.1
Exons3
Model size399
Cds size399
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig48140.11955.1prot_H-elongata_contig48140.11955.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig48140 190..2578 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930793.8321326-CDS-H-elongata_contig48140:189..3601622930793.8321326-CDS-H-elongata_contig48140:189..360Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig48140 190..360 -
1691679573.0253286-CDS-H-elongata_contig48140:189..3601691679573.0253286-CDS-H-elongata_contig48140:189..360Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig48140 190..360 -
1622930793.8463898-CDS-H-elongata_contig48140:1396..14441622930793.8463898-CDS-H-elongata_contig48140:1396..1444Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig48140 1397..1444 -
1691679573.0396223-CDS-H-elongata_contig48140:1396..14441691679573.0396223-CDS-H-elongata_contig48140:1396..1444Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig48140 1397..1444 -
1622930793.8640866-CDS-H-elongata_contig48140:2398..25781622930793.8640866-CDS-H-elongata_contig48140:2398..2578Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig48140 2399..2578 -
1691679573.0483637-CDS-H-elongata_contig48140:2398..25781691679573.0483637-CDS-H-elongata_contig48140:2398..2578Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig48140 2399..2578 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig48140.11955.1

>prot_H-elongata_contig48140.11955.1 ID=prot_H-elongata_contig48140.11955.1|Name=mRNA_H-elongata_contig48140.11955.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=133bp
QVSIHCPAFLRAPLEVTSAPLEAWATAVLFASVPEATILRAVDVLLLEKT
LVVCGRDIGMVSITATALLSLLDPFKWEGVFVPVVPLKMMDVLDSPVPAL
VGVQAPFEAPLVNPLGYALDSVVVLDLDARPAS
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mRNA from alignment at H-elongata_contig48140:190..2578-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig48140.11955.1 ID=mRNA_H-elongata_contig48140.11955.1|Name=mRNA_H-elongata_contig48140.11955.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2389bp|location=Sequence derived from alignment at H-elongata_contig48140:190..2578- (Himanthalia elongata Himel1 dioecious)
CAGGTTTCCATTCATTGCCCGGCGTTTCTTCGTGCCCCTTTGGAGGTGAC
CTCCGCCCCGCTGGAGGCGTGGGCCACCGCGGTGCTGTTCGCTTCTGTTC
CGGAGGCGACAATACTACGCGCGGTCGACGTACTTCTGCTGGAAAAAACG
CTCGTGGTCTGCGGCAGGGACATCGGCATGGTGAGGTAGATGGTCCATCC
GGAGCGTTGCCGATTCTTCTACTTGAGACTAAAGTTGTCCGTGGACAAGA
ATTGAGCCCCTTGACAGATGCGTCAAGGACACGTCCCTGAACTAAAACTA
GTATATATTTATATTTTCCCAAAAGACGCCTTGACGCACTGTCAAGAACC
TGTCAACCGACAACTATGCTTGAAAGAAGAGTGGACTCAATCTGCTTGAT
GAGAATGTTGGGCGAAGGAACGTGCGCATATTTTTCGGAGCGCCAGGACG
CACCTGCAAGCAAATTTGTGCAGAACGGAAAAATTACTTACCAGGTACAC
ACAATTAGTATAGCTCCTAAGATTTTGGTGGACCCAGCCCGACCCTAACT
CTAGCCCACACCCAATACTACTTGTAAGTAATTTTTCCGCCCTGCACAAA
TGCGCTTGCAGGTGCGTCCTGCATTTAGGGGATCTCTATATTTTATACCT
TGCGATCGGTTGACAGATTATTGACAGTGCGTCAAGGCGTCTTTTTGTAA
AATATACTATTTTTAGTGTTGGGATGTGTCCTTGACGCGTCTGTCAAGGG
GTTCAACTCTTGTCAACCGACAATTTTACTTTAGAACCTACAATACCAGT
CGGAAGCGATTTTTGGCCGTTACAACTATATCAATCGAAGTCGACGTTTA
TTCGGGGGGGGGGGTTCACATGGGTATGCTTGCTTTTGAAAAACAAAACC
TACGTAAAGCTAATATGTTGAGAAATAGTATCGTTTATATGAAATACAGC
CTGCGTCATTTTCTTCTTTTTTTTCTTCTAGTTTTGGAAGTCATTTTATT
TTTTTTAAGTTCACCGCGAACATCAAGCGAGGCTTGCGAATCGACTCCAT
CTGCCTAATTTGAAGACAAGAGTTATTACATCAACACCGCTGTTTAGCGA
CAATGTCGCCCCTCCCCCGAAATGCCTTCATCAGGTTTCCATAACTGCCA
CGGCCCTCCTGTCGCTGCTGGATCCTTTCAAGGTCAGGAGTAATTAGGGC
TCGAAGTATACTATACTAATAATATATATAGATTGTTCCAGTGTTATTGG
AATGCTTGGTCGGGGAGAGTTTTTGAAAATATACTTGTATATAAATTACA
ACAAACGATGAATCACATGTGCCGATTTTGTGTTCAAAAAGTAGTATTGT
ACACACATTCGAACCTGACGATAGCCCCTGGATTACACACCTCTCAGGAT
GCTAAGAAGGCGTGGATGTTCGGAGGGTTGGAATGAGTTCATACGTTTTG
AAAGAAAAATAATGTCTACTCACGGTGTGCTAAGGAGGCGTGAATGTTCG
GAGGGTTGGAATGAGTTTATGTTATTTTTAAGAAAAACGACATATCTAGC
AAACGTTCCAGACGCGGACTCGCACATTCCAGAAGCGGGCTCGCACGTTC
AAGATGTTCCGCATGGTGTGGTACCGCGATCCCCGGAGCTTGTGGTAAAT
GGCGGATGGCTTGAAGCAAAAGGGACACGCACTTGAAATAGTTGCGAACA
TTGATTGACGCCGTAGTCGCTGGAAGTAGCGGTTGTCGCGTGCTATTGTG
GGGTTGACTATATCAGGAAAAAACGTTTTTACATAGTAAGAGTACACGGA
GCCAGAAGCCCATCAGATATGTATTTTCCTATTTCCTTATCGCCGGCTCG
GGGCTCATCCCTCTGATCGCCGGCACGTAGAAATCGCTCACCCGTCAAAT
AGTGCTGCTTCTACTGCTGCCGCTGCTGCTGTTAATTTTTTCGGGAGCAT
GAACGACTACCTCGAACGCTACGAAACCGAGCGCGAGGCCGGTTCTGTTA
ATTAGCTGTCGTGGTGGGTGGTGTGCCAATGTCATCTCTTCCCCTCGATC
CCTTATTCCGGATTTTCTTGCAGTTGGAAGGCGTTTTTGTCGTGGGTGGT
AGACCAATGATATCTCTGTCGCTTTCAAAAGAAGCGGTCAACACAACCGC
TCCCTTGTTTATTTCTGATTTCATCTTTCCCTCTCCCCCTCGATCCCCTA
TCCCCGGATTTCTTGTAGTGGGAAGGCGTTTTTGTACCCGTGGTGCCTCT
CAAGATGATGGACGTGCTGGACAGCCCGGTGCCGGCCCTTGTAGGCGTTC
AGGCCCCGTTTGAGGCGCCCCTGGTAAACCCTCTTGGGTACGCCTTGGAC
AGTGTGGTGGTGCTCGATCTCGATGCACGTCCGGCGTCG
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Coding sequence (CDS) from alignment at H-elongata_contig48140:190..2578-

>mRNA_H-elongata_contig48140.11955.1 ID=mRNA_H-elongata_contig48140.11955.1|Name=mRNA_H-elongata_contig48140.11955.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=798bp|location=Sequence derived from alignment at H-elongata_contig48140:190..2578- (Himanthalia elongata Himel1 dioecious)
CAGGTTTCCATTCATTGCCCGGCGTTTCTTCGTGCCCCTTTGGAGGTGAC
CTCCGCCCCGCTGGAGGCGTGGGCCACCGCGGTGCTGTTCGCTTCTGTTC
CGGAGGCGACAATACTACGCGCGGTCGACGTACTTCTGCTGGAAAAAACG
CTCGTGGTCTGCGGCAGGGACATCGGCATGCAGGTTTCCATTCATTGCCC
GGCGTTTCTTCGTGCCCCTTTGGAGGTGACCTCCGCCCCGCTGGAGGCGT
GGGCCACCGCGGTGCTGTTCGCTTCTGTTCCGGAGGCGACAATACTACGC
GCGGTCGACGTACTTCTGCTGGAAAAAACGCTCGTGGTCTGCGGCAGGGA
CATCGGCATGGTTTCCATAACTGCCACGGCCCTCCTGTCGCTGCTGGATC
CTTTCAAGGTTTCCATAACTGCCACGGCCCTCCTGTCGCTGCTGGATCCT
TTCAAGTGGGAAGGCGTTTTTGTACCCGTGGTGCCTCTCAAGATGATGGA
CGTGCTGGACAGCCCGGTGCCGGCCCTTGTAGGCGTTCAGGCCCCGTTTG
AGGCGCCCCTGGTAAACCCTCTTGGGTACGCCTTGGACAGTGTGGTGGTG
CTCGATCTCGATGCACGTCCGGCGTCGTGGGAAGGCGTTTTTGTACCCGT
GGTGCCTCTCAAGATGATGGACGTGCTGGACAGCCCGGTGCCGGCCCTTG
TAGGCGTTCAGGCCCCGTTTGAGGCGCCCCTGGTAAACCCTCTTGGGTAC
GCCTTGGACAGTGTGGTGGTGCTCGATCTCGATGCACGTCCGGCGTCG
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