mRNA_H-elongata_contig46727.11775.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig46727.11775.1
Unique NamemRNA_H-elongata_contig46727.11775.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: D7G6L2_ECTSI (CTP synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G6L2_ECTSI)

HSP 1 Score: 171 bits (434), Expect = 6.910e-49
Identity = 87/110 (79.09%), Postives = 89/110 (80.91%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGK   S +  ERKG YLGKTVQ+VPH TD IQ        WIERVA IPVDGS  R DVCLIE
Sbjct:   71 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKVYQSVISKERKGEYLGKTVQVVPHITDEIQA-------WIERVAEIPVDGSNQRPDVCLIE 173
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: A0A392NTP0_9FABA (CTP synthase-like (Fragment) n=1 Tax=Trifolium medium TaxID=97028 RepID=A0A392NTP0_9FABA)

HSP 1 Score: 156 bits (395), Expect = 3.110e-47
Identity = 77/110 (70.00%), Postives = 87/110 (79.09%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            NTDAGTMSPFEHGEVFVLDDGGE DLDLGNYERFL ++L+RD+NITTGK   S +  ER+G+YLGKTVQ+VPH TD IQ        WIERVA IPVDG +   DVC+IE
Sbjct:    4 NTDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDIKLTRDNNITTGKIYQSVIEKERRGDYLGKTVQVVPHITDAIQ-------DWIERVAKIPVDGKEGPADVCVIE 106
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: A0A3N7GG79_POPTR (CTP_synth_N domain-containing protein n=3 Tax=Mesangiospermae TaxID=1437183 RepID=A0A3N7GG79_POPTR)

HSP 1 Score: 157 bits (397), Expect = 1.780e-46
Identity = 78/110 (70.91%), Postives = 88/110 (80.00%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            NTDAGTMSPFEHGEVFVLDDGGE DLDLGNYERFL ++L+RD+NITTGK   S L  ER+G+YLGKTVQ+VPH TD IQ       +WIERVA IPVDG +   DVC+IE
Sbjct:   44 NTDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDIKLTRDNNITTGKIYQSVLEKERRGDYLGKTVQVVPHITDAIQ-------EWIERVAFIPVDGKEGPADVCVIE 146
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: B7FGK3_MEDTR (CTP_synth_N domain-containing protein (Fragment) n=1 Tax=Medicago truncatula TaxID=3880 RepID=B7FGK3_MEDTR)

HSP 1 Score: 156 bits (395), Expect = 4.510e-46
Identity = 76/110 (69.09%), Postives = 88/110 (80.00%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            NTDAGTMSPFEHGEVFVLDDGGE DLDLGNYERFL ++L+RD+NITTGK   S +  ER+G+YLGKTVQ+VPH TD IQ       +WIERVA +PVDG +   DVC+IE
Sbjct:   44 NTDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDIKLTRDNNITTGKIYQSVVEKERRGDYLGKTVQVVPHITDAIQ-------EWIERVAQVPVDGKEGPADVCVIE 146
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: A0A835Z7S1_9STRA (CTP synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z7S1_9STRA)

HSP 1 Score: 154 bits (390), Expect = 1.990e-45
Identity = 78/110 (70.91%), Postives = 84/110 (76.36%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            NTDAGTMSPFEHGEVFVLDDGGE DLDLGNYERFL VRLSRDHNITTG      +  ER+G++LGKTVQ+VPH TD IQ        WIERVA  PVDGS    DVCL+E
Sbjct:   44 NTDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLSVRLSRDHNITTGTVYQEVILKERRGDHLGKTVQVVPHITDEIQ-------DWIERVAVTPVDGSGCCPDVCLVE 146
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: A0A6A5AYC2_9STRA (CTP synthase (Fragment) n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A6A5AYC2_9STRA)

HSP 1 Score: 158 bits (400), Expect = 5.800e-45
Identity = 77/110 (70.00%), Postives = 85/110 (77.27%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            N DAGTMSPFEHGEVFVLDDGGE DLDLGNYERFLG+ L++DHNITTGK     +R ER+G+YLGKTVQ+VPH TD IQ        WIERVA IPVDG     D+CLIE
Sbjct:   44 NCDAGTMSPFEHGEVFVLDDGGEGDLDLGNYERFLGITLTKDHNITTGKVYQQVIRRERRGDYLGKTVQVVPHVTDSIQA-------WIERVAQIPVDGDSTPADICLIE 146
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: A0A833U1A4_JUGRE (CTP_synth_N domain-containing protein n=1 Tax=Juglans regia TaxID=51240 RepID=A0A833U1A4_JUGRE)

HSP 1 Score: 152 bits (383), Expect = 9.980e-45
Identity = 77/110 (70.00%), Postives = 85/110 (77.27%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            NTDAGTMSPFEHGEVFVLDDGGE DLDLGNYERFL V L+RD+NITTGK   S L  ER+G+YLGKTVQ+VPH TD I+        WIE VA IPVDG +   DVC+IE
Sbjct:   44 NTDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDVTLTRDNNITTGKIYQSVLEKERRGDYLGKTVQVVPHITDAIK-------NWIEAVAVIPVDGQEGPADVCVIE 146
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: A0A453F597_AEGTS (CTP_synth_N domain-containing protein n=2 Tax=Aegilops tauschii subsp. strangulata TaxID=200361 RepID=A0A453F597_AEGTS)

HSP 1 Score: 152 bits (384), Expect = 1.130e-44
Identity = 75/110 (68.18%), Postives = 86/110 (78.18%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            NTDAGTMSPFEHGEV+VLDDGGE DLDLGNYERFL ++L+RDHNITTGK   + +  ER+G+YLGKTVQ+VPH TD IQ        WIERVA  PVDG +   DVC+IE
Sbjct:   67 NTDAGTMSPFEHGEVYVLDDGGEVDLDLGNYERFLDIKLTRDHNITTGKVYQAVIDKERRGDYLGKTVQVVPHITDEIQ-------DWIERVAIKPVDGKEGPPDVCVIE 169
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: A0A452ZZ03_AEGTS (CTP_synth_N domain-containing protein n=1 Tax=Aegilops tauschii subsp. strangulata TaxID=200361 RepID=A0A452ZZ03_AEGTS)

HSP 1 Score: 152 bits (385), Expect = 1.310e-44
Identity = 76/110 (69.09%), Postives = 86/110 (78.18%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            NTDAGTMSPFEHGEVFVLDDGGE DLDLGNYERFL ++L+RD+NITTGK   + +  ERKG+YLGKTVQ+VPH TD IQ        WIERVA  PVDG+    DVC+IE
Sbjct:   78 NTDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDIKLTRDNNITTGKIYQAVINKERKGDYLGKTVQVVPHITDEIQ-------DWIERVAMNPVDGTDKPADVCVIE 180
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BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Match: A0A453A273_AEGTS (CTP_synth_N domain-containing protein n=3 Tax=Triticinae TaxID=1648030 RepID=A0A453A273_AEGTS)

HSP 1 Score: 153 bits (386), Expect = 1.690e-44
Identity = 75/110 (68.18%), Postives = 86/110 (78.18%), Query Frame = 1 
Query:    1 NTDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLRNERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDVCLIE 330
            NTDAGTMSPFEHGEVFVLDDGGE DLDLGNYERFL ++L+RD+NITTGK   S +  ER+G YLGKTVQ+VPH T+ IQ        WIERVA +PVDG +   DVC+IE
Sbjct:   44 NTDAGTMSPFEHGEVFVLDDGGEVDLDLGNYERFLDIKLTRDNNITTGKIYQSVINKEREGEYLGKTVQVVPHITNAIQ-------DWIERVAMVPVDGQEGPADVCVIE 146
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig46727.11775.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G6L2_ECTSI6.910e-4979.09CTP synthase n=1 Tax=Ectocarpus siliculosus TaxID=... [more]
A0A392NTP0_9FABA3.110e-4770.00CTP synthase-like (Fragment) n=1 Tax=Trifolium med... [more]
A0A3N7GG79_POPTR1.780e-4670.91CTP_synth_N domain-containing protein n=3 Tax=Mesa... [more]
B7FGK3_MEDTR4.510e-4669.09CTP_synth_N domain-containing protein (Fragment) n... [more]
A0A835Z7S1_9STRA1.990e-4570.91CTP synthase n=1 Tax=Tribonema minus TaxID=303371 ... [more]
A0A6A5AYC2_9STRA5.800e-4570.00CTP synthase (Fragment) n=1 Tax=Aphanomyces astaci... [more]
A0A833U1A4_JUGRE9.980e-4570.00CTP_synth_N domain-containing protein n=1 Tax=Jugl... [more]
A0A453F597_AEGTS1.130e-4468.18CTP_synth_N domain-containing protein n=2 Tax=Aegi... [more]
A0A452ZZ03_AEGTS1.310e-4469.09CTP_synth_N domain-containing protein n=1 Tax=Aegi... [more]
A0A453A273_AEGTS1.690e-4468.18CTP_synth_N domain-containing protein n=3 Tax=Trit... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig46727contigH-elongata_contig46727:2..985 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score163.7
Seed ortholog evalue4.6e-38
Seed eggNOG ortholog2880.D7G6L2
Preferred nameCTPS2
KEGG rclassRC00010,RC00074
KEGG koko:K01937
KEGG ReactionR00571,R00573
KEGG Pathwayko00240,ko01100,map00240,map01100
KEGG ModuleM00052
GOsGO:0000322,GO:0000323,GO:0000324,GO:0001775,GO:0002164,GO:0002376,GO:0002520,GO:0003674,GO:0003824,GO:0003883,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0005829,GO:0006139,GO:0006206,GO:0006207,GO:0006213,GO:0006220,GO:0006221,GO:0006241,GO:0006629,GO:0006644,GO:0006725,GO:0006753,GO:0006793,GO:0006796,GO:0006807,GO:0007275,GO:0008150,GO:0008152,GO:0008283,GO:0008610,GO:0008654,GO:0009058,GO:0009112,GO:0009116,GO:0009117,GO:0009119,GO:0009141,GO:0009142,GO:0009147,GO:0009148,GO:0009163,GO:0009165,GO:0009199,GO:0009201,GO:0009208,GO:0009209,GO:0009218,GO:0009220,GO:0009259,GO:0009260,GO:0009791,GO:0009987,GO:0015949,GO:0016874,GO:0016879,GO:0018130,GO:0019438,GO:0019637,GO:0019693,GO:0019856,GO:0030097,GO:0032501,GO:0032502,GO:0032943,GO:0034404,GO:0034641,GO:0034654,GO:0035166,GO:0035167,GO:0042098,GO:0042100,GO:0042110,GO:0042113,GO:0042221,GO:0042455,GO:0042493,GO:0042802,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044271,GO:0044281,GO:0044283,GO:0044424,GO:0044444,GO:0044464,GO:0045321,GO:0046036,GO:0046112,GO:0046131,GO:0046132,GO:0046134,GO:0046390,GO:0046483,GO:0046649,GO:0046651,GO:0048513,GO:0048534,GO:0048542,GO:0048569,GO:0048731,GO:0048732,GO:0048856,GO:0050896,GO:0055086,GO:0070661,GO:0071704,GO:0072527,GO:0072528,GO:0090407,GO:0097268,GO:1901135,GO:1901137,GO:1901293,GO:1901360,GO:1901362,GO:1901564,GO:1901566,GO:1901576,GO:1901657,GO:1901659
EggNOG free text desc.'de novo' CTP biosynthetic process
EggNOG OGsCOG0504@1,KOG2387@2759
EC6.3.4.2
COG Functional cat.F
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Hectar predicted targeting categoryother localisation
Exons3
Model size330
Cds size312
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig46727.11775.1prot_H-elongata_contig46727.11775.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig46727 2..967 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930773.3229723-CDS-H-elongata_contig46727:1..741622930773.3229723-CDS-H-elongata_contig46727:1..74Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46727 2..74 -
1691679563.9078276-CDS-H-elongata_contig46727:1..741691679563.9078276-CDS-H-elongata_contig46727:1..74Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46727 2..74 -
1622930773.33905-CDS-H-elongata_contig46727:323..4091622930773.33905-CDS-H-elongata_contig46727:323..409Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46727 324..409 -
1691679563.9266243-CDS-H-elongata_contig46727:323..4091691679563.9266243-CDS-H-elongata_contig46727:323..409Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46727 324..409 -
1622930773.356895-CDS-H-elongata_contig46727:814..9671622930773.356895-CDS-H-elongata_contig46727:814..967Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46727 815..967 -
1691679563.9374826-CDS-H-elongata_contig46727:814..9671691679563.9374826-CDS-H-elongata_contig46727:814..967Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46727 815..967 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930773.3759644-UTR-H-elongata_contig46727:967..9851622930773.3759644-UTR-H-elongata_contig46727:967..985Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig46727 968..985 -
1691679563.9484198-UTR-H-elongata_contig46727:967..9851691679563.9484198-UTR-H-elongata_contig46727:967..985Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig46727 968..985 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig46727.11775.1

>prot_H-elongata_contig46727.11775.1 ID=prot_H-elongata_contig46727.11775.1|Name=mRNA_H-elongata_contig46727.11775.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=104bp
MSPFEHGEVFVLDDGGEADLDLGNYERFLGVRLSRDHNITTGKARYSDLR
NERKGNYLGKTVQIVPHFTDVIQVRLFGLEQWIERVASIPVDGSKIRHDV
CLIE
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mRNA from alignment at H-elongata_contig46727:2..985-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig46727.11775.1 ID=mRNA_H-elongata_contig46727.11775.1|Name=mRNA_H-elongata_contig46727.11775.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=984bp|location=Sequence derived from alignment at H-elongata_contig46727:2..985- (Himanthalia elongata Himel1 dioecious)
AACACGGACGCCGGAACCATGTCTCCTTTCGAGCACGGCGAAGTGTTCGT
CCTGGACGACGGCGGGGAAGCGGATTTGGATCTTGGCAATTACGAGAGGT
TTCTCGGCGTTCGTTTGTCGAGAGACCACAATATCACCACCGGCAAGGCA
AGATATTCGGATTTGCGCAACGTCGCCGTGTTTCGCGTGCCTGGTGGGGG
AAGAAGCGCCACCGTATTTGTCTTCGTATTTGCCGTGCTCGTCTCGCGCC
TTTGCGTTATACGCGTTATAGATCATGTAAAGTGTCGTTCGCTGTGCACA
AGGCGCCTATGTAGAGCGCAATGAAAGCGTCTGGTAGATAGAAGACAACG
TGTGAGGAGCAATCAACCAACCGCATAAATGATACAAACTGAATGTTCAG
TCGGGATGGAATCCAGCTCTCTAGCGGGCCCGTCCAGCCTACCAACTGCT
ACGGCACTTGCAACCCTGAAAGCACGTGTGAATAAGTATTCATGTGCGGT
TTAGACGTAGTTGCCACTTACTCTGTGCATGGTGACGACATGCTGTCGAC
AGGTGTACCTAAAAGTAATCTTCAAGGAACGAAAGGGAAATTACTTGGGC
AAAACGGTACAAATCGTACCGCATTTCACGGATGTAATACAGGTCAGGCT
CTTTGGCCTCGAGTGCAGACATGGCCCTCGGTGTAAGGAATGCATAGATA
CATCAATCGTGTTTCAAGAATTCAACGTGATCGGAAGGCTCCATACAATA
TTACTACTTTGTACCCGTTACCGTTGTTTTATATTAACTTTGCGACGTGG
GAAAAGCTCAGGGCGCACATTGCAGTGCTTACGTGACACTGTTCATGAGC
TTCAATTTCGCGAGGTTTACTGACATGTATACCTCGTCACTGGCGTTGGT
GATCTACGCAGGCAATGGATAGAGCGGGTCGCCAGTATTCCCGTTGACGG
CTCTAAAATCCGACATGACGTGTGCCTCATCGAA
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Coding sequence (CDS) from alignment at H-elongata_contig46727:2..985-

>mRNA_H-elongata_contig46727.11775.1 ID=mRNA_H-elongata_contig46727.11775.1|Name=mRNA_H-elongata_contig46727.11775.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=624bp|location=Sequence derived from alignment at H-elongata_contig46727:2..985- (Himanthalia elongata Himel1 dioecious)
ATGTCTCCTTTCGAGCACGGCGAAGTGTTCGTCCTGGACGACGGCGGGGA
AGCGGATTTGGATCTTGGCAATTACGAGAGGTTTCTCGGCGTTCGTTTGT
CGAGAGACCACAATATCACCACCGGCAAGGCAAGATATTCGGATTTGCGC
AACATGTCTCCTTTCGAGCACGGCGAAGTGTTCGTCCTGGACGACGGCGG
GGAAGCGGATTTGGATCTTGGCAATTACGAGAGGTTTCTCGGCGTTCGTT
TGTCGAGAGACCACAATATCACCACCGGCAAGGCAAGATATTCGGATTTG
CGCAACGAACGAAAGGGAAATTACTTGGGCAAAACGGTACAAATCGTACC
GCATTTCACGGATGTAATACAGGTCAGGCTCTTTGGCCTCGAGAACGAAA
GGGAAATTACTTGGGCAAAACGGTACAAATCGTACCGCATTTCACGGATG
TAATACAGGTCAGGCTCTTTGGCCTCGAGCAATGGATAGAGCGGGTCGCC
AGTATTCCCGTTGACGGCTCTAAAATCCGACATGACGTGTGCCTCATCGA
AGCAATGGATAGAGCGGGTCGCCAGTATTCCCGTTGACGGCTCTAAAATC
CGACATGACGTGTGCCTCATCGAA
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