mRNA_H-elongata_contig46561.11753.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig46561.11753.1
Unique NamemRNA_H-elongata_contig46561.11753.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig46561.11753.1 vs. uniprot
Match: A0A6H5J9X3_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J9X3_9PHAE)

HSP 1 Score: 122 bits (306), Expect = 1.000e-25
Identity = 69/152 (45.39%), Postives = 87/152 (57.24%), Query Frame = 1 
Query:   13 CMLPLEDPCTPGTSSSNINTGIGLFYSLACNSTDAESDSVPGCNTDPTNLCRDCFFNTTLYQVKNPGEELPDYVDCPCCVLEEYRKDSAVNEDGPLCENFTPTP-------VRAGDAFYTKPFD---FRDAQRKYGGGEGIALAALVLLRGL 438
            C+LP   PCT  TS SN N GIG +Y  AC   + E + + GCN D ++LCR CFFNT LY  K+P E+LPDY DCPCCVL+ Y +D A N DG  C + +P P       +  G+    + FD   F D   +   GE  AL+AL LL  L
Sbjct:  412 CILPESKPCTEATSFSNTNEGIGDYYDPACELVEGE-EQLGGCNADASSLCRMCFFNTPLYVAKHPDEDLPDYPDCPCCVLDIYEQD-AFNLDGQRCVSASPAPSSPALTEIPVGE-ITPRGFDDLSFDDGGWEMNAGEVAALSALGLLAAL 560
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BLAST of mRNA_H-elongata_contig46561.11753.1 vs. uniprot
Match: A0A6H5J9W9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J9W9_9PHAE)

HSP 1 Score: 120 bits (300), Expect = 1.020e-24
Identity = 57/114 (50.00%), Postives = 74/114 (64.91%), Query Frame = 1 
Query:    1 DGMECMLPLEDPCTPGTSS-SNINTGIGLFYSLACNS---TDAESDSVP-GCNTDPTNLCRDCFFNTTLYQVKNPGEELPDYVDCPCCVLEEYRKDSAVNEDGPLCENFTPTPV 327
            +G ECM+P   PCT   S+ S+ N G+G FY   C +   T +E   +P GC+    + CR C FNT  +Q KNPGE+LPD VDCPCCVL+ Y++D+A+NEDG  CE  TP PV
Sbjct:  535 EGQECMIPEGAPCTSTASTFSHTNQGVGSFYDPTCAAAAATASEEGELPEGCDPSSADSCRFCLFNTPRWQDKNPGEDLPDLVDCPCCVLDIYKEDTAINEDGVRCEWMTPAPV 648
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BLAST of mRNA_H-elongata_contig46561.11753.1 vs. uniprot
Match: D8LE44_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE44_ECTSI)

HSP 1 Score: 105 bits (261), Expect = 4.780e-21
Identity = 47/103 (45.63%), Postives = 69/103 (66.99%), Query Frame = 1 
Query:    1 DGMECMLPLEDPCTPGTSS-SNINTGIGLFYSLACNS---TDAESDSVP-GCNTDPTNLCRDCFFNTTLYQVKNPGEELPDYVDCPCCVLEEYRKDSAVNEDG 294
            +G ECM+P E PCT   ++ ++ N G+G +Y   C +   T +E   +P GC+    + CR C FNT  +Q KNPGE+LPD VDCPCCV++ Y++D+A++EDG
Sbjct:  213 EGQECMIPEEAPCTSTAATIAHTNQGVGSYYDPTCAAAAATASEEGELPEGCDPSSADSCRFCLFNTPRWQDKNPGEDLPDLVDCPCCVVDVYKEDTAISEDG 315
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BLAST of mRNA_H-elongata_contig46561.11753.1 vs. uniprot
Match: A0A6H5JBT9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBT9_9PHAE)

HSP 1 Score: 99.8 bits (247), Expect = 2.830e-18
Identity = 50/139 (35.97%), Postives = 71/139 (51.08%), Query Frame = 1 
Query:   13 CMLPLEDPCTPGTSSSNINTGIGLFYSLACNSTDAESDSVPGCNTDPTNLCRDCFFNTTLYQVKNPGE-ELPDYVDCPCCVLEEYRKDSAVNEDGPLCENFTPTPVRAGDAFYTKPFDFRDAQRKYGGGEGIALAALVL 426
            C  PL+ PCT  T  S+IN G+G +Y   C        S  GC    + LCR C+FN T+ +  N  + ++PDY +CPCCV++EY + +        C   TP+P +       + + F D      GGEG A+AAL L
Sbjct:  469 CTWPLDGPCTTETLFSDINRGLGAYYDPGC--------SGAGCGVGDSGLCRTCYFNLTMGEEGNDDDVDMPDYPECPCCVMDEYDRSAD------RCVGATPSPAQGTGTEALETYSFEDKPLDMSGGEGFAVAALAL 593
[Close]
BLAST of mRNA_H-elongata_contig46561.11753.1 vs. uniprot
Match: D8LE45_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE45_ECTSI)

HSP 1 Score: 60.8 bits (146), Expect = 3.290e-8
Identity = 23/35 (65.71%), Postives = 28/35 (80.00%), Query Frame = 1 
Query:  172 CFFNTTLYQVKNPGEELPDYVDCPCCVLEEYRKDS 276
            CFFNT LY  K+P E+LPDY DCPCCVL+ Y +D+
Sbjct:    2 CFFNTPLYVEKHPDEDLPDYPDCPCCVLDIYEQDA 36
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig46561.11753.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
A0A6H5J9X3_9PHAE1.000e-2545.39Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5J9W9_9PHAE1.020e-2450.00Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LE44_ECTSI4.780e-2145.63Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JBT9_9PHAE2.830e-1835.97Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LE45_ECTSI3.290e-865.71Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig46561contigH-elongata_contig46561:247..4224 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionexpressed unknown protein
Ec32 orthologEc-05_004120.1
Exons5
Model size1811
Cds size591
Stop1
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930770.8828611-UTR-H-elongata_contig46561:246..13111622930770.8828611-UTR-H-elongata_contig46561:246..1311Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig46561 247..1311 -
1691679562.5967593-UTR-H-elongata_contig46561:246..13111691679562.5967593-UTR-H-elongata_contig46561:246..1311Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig46561 247..1311 -
1622930770.8966842-UTR-H-elongata_contig46561:1364..15131622930770.8966842-UTR-H-elongata_contig46561:1364..1513Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig46561 1365..1513 -
1691679562.6088014-UTR-H-elongata_contig46561:1364..15131691679562.6088014-UTR-H-elongata_contig46561:1364..1513Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig46561 1365..1513 -
1622930770.9872208-UTR-H-elongata_contig46561:4218..42241622930770.9872208-UTR-H-elongata_contig46561:4218..4224Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig46561 4219..4224 -
1691679562.6565852-UTR-H-elongata_contig46561:4218..42241691679562.6565852-UTR-H-elongata_contig46561:4218..4224Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig46561 4219..4224 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930770.9079647-CDS-H-elongata_contig46561:1513..16811622930770.9079647-CDS-H-elongata_contig46561:1513..1681Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46561 1514..1681 -
1691679562.617626-CDS-H-elongata_contig46561:1513..16811691679562.617626-CDS-H-elongata_contig46561:1513..1681Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46561 1514..1681 -
1622930770.9227393-CDS-H-elongata_contig46561:1787..19151622930770.9227393-CDS-H-elongata_contig46561:1787..1915Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46561 1788..1915 -
1691679562.6257274-CDS-H-elongata_contig46561:1787..19151691679562.6257274-CDS-H-elongata_contig46561:1787..1915Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46561 1788..1915 -
1622930770.944743-CDS-H-elongata_contig46561:2906..30591622930770.944743-CDS-H-elongata_contig46561:2906..3059Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46561 2907..3059 -
1691679562.6338081-CDS-H-elongata_contig46561:2906..30591691679562.6338081-CDS-H-elongata_contig46561:2906..3059Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46561 2907..3059 -
1622930770.9637992-CDS-H-elongata_contig46561:4076..42181622930770.9637992-CDS-H-elongata_contig46561:4076..4218Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46561 4077..4218 -
1691679562.6425266-CDS-H-elongata_contig46561:4076..42181691679562.6425266-CDS-H-elongata_contig46561:4076..4218Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig46561 4077..4218 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig46561.11753.1prot_H-elongata_contig46561.11753.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig46561 1514..4218 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig46561.11753.1

>prot_H-elongata_contig46561.11753.1 ID=prot_H-elongata_contig46561.11753.1|Name=mRNA_H-elongata_contig46561.11753.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=197bp
MECMLPLEDPCTPGTSSSNINTGIGLFYSLACNSTDAESDSVPGCNTDPT
NLCRDCFFNTTLYQVKNPGEELPDYVDCPCCVLEEYRKDSAVNEDGPLCE
NFTPTPVRAGDAFYTKPFDFRDAQRKYGGGEGIALAALVLLRGLFRSLFG
TGANDNMLKIFEVRFVLDVVVALACVYPEARRVILEKTRILRCFAL*
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mRNA from alignment at H-elongata_contig46561:247..4224-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig46561.11753.1 ID=mRNA_H-elongata_contig46561.11753.1|Name=mRNA_H-elongata_contig46561.11753.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=3978bp|location=Sequence derived from alignment at H-elongata_contig46561:247..4224- (Himanthalia elongata Himel1 dioecious)
GACGGTATGGAGTGCATGCTGCCGTTGGAAGACCCGTGCACGCCAGGAAC
TTCTTCGTCTAACATCAACACAGGCATAGGGTTGTTCTACAGCCTGGCTT
GCAACAGCACTGACGCCGAATCGGATTCCGTGCCCGGGTGCAACACGGGT
GGGTTTTTCTGGAGGTGGCTACTGAGTGTGTTTAGAAAAGAAACAAGAGA
AGCACGGAGGGACAGGTGTCAAAATCACGCTCGCCATCACTTTTGTTTTA
TTTATTTTTTGCGAGAGCGGCACGACGTTCTCTTGTGTTTTGGTACGCGG
TTGTTTTTTTAATCCTTAGAGTTTACTCGCAAAGTTACGCCCGACGCCAT
CCCCTGACGGTGACGATCCAAAATTAATCACTTAAGACTCTTCTCCTCCT
ATTCACATTTGAGGTGCCGCGTAGGTTACGGGGAGTAAAATACACCCCCG
CAGACGGAAATCCCCCCCCCCCCTTGTTAACCCATGGCATGTCAGGTTTG
GTGTAGCTAACGCGAGGATGATGATCTAACGGCTGGTATCACTGTTGTGA
AACATAAGCCTGACCGGCGAAAACTGGCATTGTTGAGATTCGTGATCTAA
ATCCGTTAGAATACGACGTGCGTTTAGCACAAGTACGCTCCATCACCCCC
CCTTGAATTTCTCAAGAACTCGGCCGTTGTTCACACGAAAAAATACGGAT
TTTGAAAAACTCTCGCGAAGGTCTCCTTGATTTTGTGGGGGTTTAACCCC
ATGGGCGCTTGCGCTGTCATATACATATCCCTACCTCTCCTCTCCCCAAC
CCCCCGCGCCATTGTGTTGGAAGACATATCCGCCTGAGGACATAAGTAAT
CATTCCCCTTTTAATCGAGGATTTGTTCCACTTCATCCCCTACGGCTTCA
CATCTCGGTCGATAAAGCGCCTTCCCGCGAGGCGCTGAAACTTCGAACTA
GCTTGTCTGCAGTACTAATCACGTTCAGCGAATTAATAGGTTCCAGAAAT
GTGGCAGTCTGGCAGAATATATAGGGCTGCGGATCGGCCAAACATGTGCG
TCACCGAAGCGTTTAACAGTGTTTTCGGTTCGGTTATGCGGGAAGTTTTC
ACAGTCCCGTCTCGTGCGGCGTTATGTTTCTGCTACCGTCTTGTGTGTCG
ATCTCGGCCCTGCAGACCCCACGAACTTGTGCCGGGACTGCTTCTTCAAT
ACCACTCTCTACCAGGTGAAAAACCCTGGCGAGGAGCTCCCGGACTACGT
TGACTGTCCCTGCTGCGTATTGGAAGAGTATCGCAAAGACAGCGCCGTGA
ACGAGGACGGGCCGCTATGTGAGACATCGTCCTACTTATCATTGCTTGTT
TGTTTCGTCTGATATTTGTGGGTTTTCATTTTATATTTGAGGTTTTTATT
TTCAACGATATATATATATATAATATATATATATTATTTTTTTCCCGCTT
CTTGTCACTTTGTACCGATGGGACAAATCAATTTGCTAGGTTTCCATTAC
CACCTTTGACCGTAACGGATATATCTATGCCCTATAGCCCTCTCCGGGCA
ATGCCAACGATAAAAAGCTTATGTAATTTTAATAACATCGCCTCTACACA
TCAACTAATGGTTGTACGCACGAAATGGCGCTTTCCGTTACACAAGGGCG
TTTGATTTCAAAACTGCTGAACCACTGGTTGTGTAGGCTACTGATAACTA
CCGTACTTGAACAATAAAGTGTCTGATTGGGACGTTTGTGTGTTTTGCTT
TGTTTCGCTTCGACTTTGGGGTTTAAAGCCTTGCTCATAGTCTGATGCAA
AAGCCCTTAAAATCTCGGAATGGGTTTTGTAGCCAGGTTTTCCTCAGCTT
CGTGGCCATCTGGGTAGATCATTATATAGGATATGTAATTAGTGCGTCGG
AAACCTCGCTCTCATTCCCCGTGGTGTTCATATTTTCAAACTTGATACAG
ATGGATTGACGCGGTTGTAGACGACCACATAGCTGTGCGGACTCGCATAC
GGCATTGCCAGTACACTAAACGCCGTACACACGACTGGCATAAGAAAACG
GCCTGAAGCACGAACCATTTCGCAGGAAGATCCGTCCGATGGACCGTTTT
AGGGACTTAATCTGCCTATATACGCCTATGTTCATCTGGAACAGCAGCAG
ACGAAAAGTACTGGCCTGTATAAACGGGCTGAGCCGCCGTGCAGGCGGTA
TCGAATCACAGCTTTAACGAAGCGTCTCTCCGCGCCCCGACCCTGTTCCC
CATCTATGTGGCCTACCCTTGCTGCGTCTACACCCTCGGCGAACCCTTGT
CTTCGACAGGCGAGAACTTTACCCCTACACCAGTCAGGGCGGGAGACGCG
TTCTACACAAAACCTTTCGACTTTAGGGACGCGCAGAGAAAATACGGGGG
CGGCGAGGGAATAGCGCTCGCGGCGCTTGTGCTGCTGGGTGTGTTTACTT
TTTCTCCGTTTTGCGCAAAGTGAAAACGCGCACGCGCGCGCGAGCTTTGT
CGGTCATGCTTGGGGTCTCACTGGCGTTTGCTCTCGTTTTGTTCGTGGTT
TATTTCGATCTTTATTCGGCACGGGTGCCAACGACAACATGTTAAAAATA
TTTGAAGTTCGATTCGTTTTGGATGTAGTTGTTGCTTTGGCTTGTGTGTA
CCCCGAGGCGAGGCGCGTCATTTTGGAGAAGACGCGTATTTTGCGGTGTT
TCGCTTTATAGAGAACCACGCGGCACGCTTCGGAGCAGCCATCCCACCGT
CAAATATTGTTACTGGTACCTGGAGGCATAAGGATGTGTTTCTTGCTTCA
AAAGGAAAAAGGATGAATCTTGGCGGCGCCATCGATATCATACGAAGTAT
ATGATGCTTCCCTCGTGCTGCCGCGCTGTAGCCCCCGCGGAGTTGAACGC
TCGGCTTGCGCGCGACGTCACCGTGTACGCAAACGCCAGCGGCGTTTTCC
TGTGATTCCCACGATACACTAAGCGATGAGCTCCTATCCTTTGGTTTTCT
TGTAATAGATCGTACCCAAGTAATTTCTACACAGAGTCAAATCCTCTCGA
AAAGGACTGGAACCCCTGTCTTCCCCTAGGTTGGTGGGCTAAGGTTGTTA
AGAGGCATGTAAAAGCTACACATACATGACGATCATGTCCTCCCTCATGC
GCCTGGTCTCTGGTTCCAGCTGTACTATCTTGAGAGAGATGCACTGCGGT
GTCTGGCCTTCCGTCGTGGGGTTCATTGTGGAGGTCGAAGTGCAGTTATA
TTTTTCGTTTTACATTACGGTGATGTAGTTTGCCGCGTGCGTGTAGTCTA
GAGCTATCATTGTTATGTGGGAGTGGGAGTAACGAGTGTAAAGTTAGCAT
GTTGATTCCAAGAGTCAGCAGACACGCGGGGCACTTTGCTAGTCGATCTG
CGTGACGCTGCGTGGTTTCGTATCTTGCGAAGATACACCCGGTGTGTCAT
ACGCGTTCCCATCTTCTTTTTGATGTGTCTTGCGCGTATCGCAAATTTCT
TGAATAGCGTGATTTTTTCGTCGCAGAGATTAAAGGTTTCTTAGTTTGTT
TTTTGTTAGTGTACTTGTCGGAAGAGCAGCAGAAAATTACACCCAGCTTT
CCCTCGAACGACACAGGATTGACTTTGTCCGGTGTATTTTGACGAGCGAC
CTGTGGCGTATGGAATGTGGCGGGTTTGCACGGGGTGATGAATCTAGCTG
TGCCGGCGGGCATTCCATTCATAGTGTTGGGACTCGGTGTAAGTAAAACC
GAGGACATGAGCTTCGACTCGATGTATAATACGGCCATGTTCTACGTGGA
CGTTGGTACGGTTATTTCGAGATTCTAACTCTGTTGATGACTGTCGTGTA
GAGCGTATATGAAAATGCTTATCTATTGTTATCTCGAACCTATGGTACGT
TTAAGGTAGAGGTGCGACCCGCCGATGATGTTTGTGAAGCCGGGAGACTC
CAATCATCAGCGGGAGATATCAGGGATC
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Coding sequence (CDS) from alignment at H-elongata_contig46561:247..4224-

>mRNA_H-elongata_contig46561.11753.1 ID=mRNA_H-elongata_contig46561.11753.1|Name=mRNA_H-elongata_contig46561.11753.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=1182bp|location=Sequence derived from alignment at H-elongata_contig46561:247..4224- (Himanthalia elongata Himel1 dioecious)
ATGGAGTGCATGCTGCCGTTGGAAGACCCGTGCACGCCAGGAACTTCTTC
GTCTAACATCAACACAGGCATAGGGTTGTTCTACAGCCTGGCTTGCAACA
GCACTGACGCCGAATCGGATTCCGTGCCCGGGTGCAACACGGATGGAGTG
CATGCTGCCGTTGGAAGACCCGTGCACGCCAGGAACTTCTTCGTCTAACA
TCAACACAGGCATAGGGTTGTTCTACAGCCTGGCTTGCAACAGCACTGAC
GCCGAATCGGATTCCGTGCCCGGGTGCAACACGGACCCCACGAACTTGTG
CCGGGACTGCTTCTTCAATACCACTCTCTACCAGGTGAAAAACCCTGGCG
AGGAGCTCCCGGACTACGTTGACTGTCCCTGCTGCGTATTGGAAGAGTAT
CGCAAAGACAGCGCCGTGAACGAGGACGGGCCGCTATACCCCACGAACTT
GTGCCGGGACTGCTTCTTCAATACCACTCTCTACCAGGTGAAAAACCCTG
GCGAGGAGCTCCCGGACTACGTTGACTGTCCCTGCTGCGTATTGGAAGAG
TATCGCAAAGACAGCGCCGTGAACGAGGACGGGCCGCTATGCGAGAACTT
TACCCCTACACCAGTCAGGGCGGGAGACGCGTTCTACACAAAACCTTTCG
ACTTTAGGGACGCGCAGAGAAAATACGGGGGCGGCGAGGGAATAGCGCTC
GCGGCGCTTGTGCTGCTGGCGAGAACTTTACCCCTACACCAGTCAGGGCG
GGAGACGCGTTCTACACAAAACCTTTCGACTTTAGGGACGCGCAGAGAAA
ATACGGGGGCGGCGAGGGAATAGCGCTCGCGGCGCTTGTGCTGCTGCGTG
GTTTATTTCGATCTTTATTCGGCACGGGTGCCAACGACAACATGTTAAAA
ATATTTGAAGTTCGATTCGTTTTGGATGTAGTTGTTGCTTTGGCTTGTGT
GTACCCCGAGGCGAGGCGCGTCATTTTGGAGAAGACGCGTATTTTGCGGT
GTTTCGCTTTATAGCGTGGTTTATTTCGATCTTTATTCGGCACGGGTGCC
AACGACAACATGTTAAAAATATTTGAAGTTCGATTCGTTTTGGATGTAGT
TGTTGCTTTGGCTTGTGTGTACCCCGAGGCGAGGCGCGTCATTTTGGAGA
AGACGCGTATTTTGCGGTGTTTCGCTTTATAG
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