mRNA_H-elongata_contig167030.4192.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig167030.4192.1
Unique NamemRNA_H-elongata_contig167030.4192.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: A0A6H5JJL6_9PHAE (SEC7 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JJL6_9PHAE)

HSP 1 Score: 167 bits (423), Expect = 6.640e-46
Identity = 90/107 (84.11%), Postives = 99/107 (92.52%), Query Frame = 1 
Query:    1 GDAEDQNKSVLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLGRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
             DA +Q + VLV++MRFLSLLSDPRSNG AE VLSLSLINIALEAGG+DLGRIP+LVRVMRGDLCKHLLQNSQ +DL+VLSLTLRVVFNLFNSIKD LK+QLEVFLT
Sbjct:  386 SDAAEQ-EGVLVKLMRFLSLLSDPRSNGSAECVLSLSLINIALEAGGADLGRIPSLVRVMRGDLCKHLLQNSQTDDLDVLSLTLRVVFNLFNSIKDHLKVQLEVFLT 491
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: D7FVF3_ECTSI (GBF1, ArfGEF protein of the BIG/GBF subfamily n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVF3_ECTSI)

HSP 1 Score: 166 bits (421), Expect = 1.230e-45
Identity = 90/107 (84.11%), Postives = 98/107 (91.59%), Query Frame = 1 
Query:    1 GDAEDQNKSVLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLGRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
            G AE +   VLV++MRFLSLLSDPRSNG AE VLSLSLINIALEAGG+DLGRIP+LVRVMRGDLCKHLLQNSQ +DL+VLSLTLRVVFNLFNSIKD LK+QLEVFLT
Sbjct:  387 GTAEQEG--VLVKLMRFLSLLSDPRSNGSAECVLSLSLINIALEAGGADLGRIPSLVRVMRGDLCKHLLQNSQTDDLDVLSLTLRVVFNLFNSIKDHLKVQLEVFLT 491
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: A0A2D4CF02_PYTIN (SEC7 domain-containing protein (Fragment) n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CF02_PYTIN)

HSP 1 Score: 112 bits (280), Expect = 1.270e-26
Identity = 64/107 (59.81%), Postives = 75/107 (70.09%), Query Frame = 1 
Query:    1 GDAEDQNKSVLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLGRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
            G A     ++L + + FL+ L  P SN  A  VL L LINI LE  G+ LG   ALV V++GDLCK LLQNS+ E+L VLSLTLRVVFNLFNSIKD LK+QLEVF T
Sbjct:  221 GRANGYGVALLEQTLTFLARLIAPASNDDATCVLGLRLINIVLETAGASLGAHAALVSVLQGDLCKFLLQNSETEELAVLSLTLRVVFNLFNSIKDHLKVQLEVFFT 327
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: A0A8K1CH01_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CH01_PYTOL)

HSP 1 Score: 110 bits (274), Expect = 8.210e-26
Identity = 60/98 (61.22%), Postives = 72/98 (73.47%), Query Frame = 1 
Query:   28 VLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLGRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
            +L R+++FLS L  P +   A  V  L LINI LE  G+ LG  P LV V++GDL K+LLQNS+ EDL +LSLTLRVVFNLFNSIKD LK+QLEVF T
Sbjct:  237 LLERILQFLSTLISPTAKDEATCVFGLRLINIVLETAGTGLGSHPGLVSVLQGDLSKYLLQNSETEDLAILSLTLRVVFNLFNSIKDHLKVQLEVFFT 334
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: A0A225WXV2_9STRA (SEC7 domain-containing protein n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225WXV2_9STRA)

HSP 1 Score: 109 bits (272), Expect = 1.530e-25
Identity = 59/98 (60.20%), Postives = 72/98 (73.47%), Query Frame = 1 
Query:   28 VLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLGRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
            +L R++ F+S L  P  N  A  VL L LIN+ LE  G+ LG  P+LV V++GDL K LLQNS+ E+L +LSLTLRVVFNLFNSIKD LK+QLEVF T
Sbjct:  263 LLERILHFISGLISPTENEEATCVLGLRLINVVLETAGTGLGNHPSLVSVLQGDLSKFLLQNSETEELGILSLTLRVVFNLFNSIKDHLKVQLEVFFT 360
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: A0A6A3JS86_9STRA (SEC7 domain-containing protein n=4 Tax=Phytophthora TaxID=4783 RepID=A0A6A3JS86_9STRA)

HSP 1 Score: 108 bits (270), Expect = 2.850e-25
Identity = 59/98 (60.20%), Postives = 71/98 (72.45%), Query Frame = 1 
Query:   28 VLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLGRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
            +L R++ F+S L  P  N  A  VL L LIN+ LE  G+ LG  P LV V++GDL K LLQNS+ E+L +LSLTLRVVFNLFNSIKD LK+QLEVF T
Sbjct:  260 LLERILHFVSGLISPSENEEATCVLGLRLINVVLETAGTGLGNHPGLVSVLQGDLSKFLLQNSETEELGILSLTLRVVFNLFNSIKDHLKVQLEVFFT 357
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: A0A7S2WBL3_9STRA (Hypothetical protein n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2WBL3_9STRA)

HSP 1 Score: 108 bits (270), Expect = 2.860e-25
Identity = 62/118 (52.54%), Postives = 78/118 (66.10%), Query Frame = 1 
Query:    7 AEDQNKSVLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLG-------------RIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
            AE     VLV+V+ FLS L+DP  N   + + +L LINI LE+GG  LG                 ++ V++GDLCKHLLQNSQ  DL+VLSLTLRV+FNLFN++K  LK+QLEVFLT
Sbjct:  319 AESYGLPVLVKVLEFLSNLTDPLRNTEPDRIFALQLINIILESGGETLGTWSPPDEPNDPRAEASPIITVLQGDLCKHLLQNSQSLDLSVLSLTLRVIFNLFNAMKGHLKVQLEVFLT 436
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: A0A3F2RJI9_9STRA (SEC7 domain-containing protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3F2RJI9_9STRA)

HSP 1 Score: 107 bits (268), Expect = 5.310e-25
Identity = 58/98 (59.18%), Postives = 71/98 (72.45%), Query Frame = 1 
Query:   28 VLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLGRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
            +L R++ F+S L  P  N  A  VL L LIN+ LE  G+ LG  P LV V++GDL K LLQNS+ E+L +LSLTLRVVFNLFNSIKD LK+QLE+F T
Sbjct:  240 LLERILHFISGLISPTENEEATCVLGLRLINVVLETAGTGLGNHPCLVSVLQGDLSKFLLQNSETEELAILSLTLRVVFNLFNSIKDHLKVQLEIFFT 337
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: W2PX92_PHYPN (SEC7 domain-containing protein n=12 Tax=Phytophthora TaxID=4783 RepID=W2PX92_PHYPN)

HSP 1 Score: 107 bits (266), Expect = 9.880e-25
Identity = 58/98 (59.18%), Postives = 70/98 (71.43%), Query Frame = 1 
Query:   28 VLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLGRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
            +L R++ F+S L  P  N     VL L LIN+ LE  G+ LG  P LV V++GDL K LLQNS+ E+L +LSLTLRVVFNLFNSIKD LK+QLEVF T
Sbjct:  262 LLERILHFISGLISPTENEEMTCVLGLRLINVVLETAGTGLGNHPCLVSVLQGDLSKFLLQNSETEELGILSLTLRVVFNLFNSIKDHLKVQLEVFFT 359
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BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Match: A0A3M6VFJ1_9STRA (SEC7 domain-containing protein n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VFJ1_9STRA)

HSP 1 Score: 106 bits (265), Expect = 1.350e-24
Identity = 57/98 (58.16%), Postives = 70/98 (71.43%), Query Frame = 1 
Query:   28 VLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDLGRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKIQLEVFLT 321
            +L R++ F+S L+ P  N     V  L LIN+ LE  G+ LG  P LV V++GDL K LLQNS+ E+L +LSLTLRVVFNLFNSIKD LK+QLEVF T
Sbjct:  270 LLERILHFISSLTSPMENEEMTCVQGLRLINVVLETAGTGLGNHPCLVSVLQGDLSKFLLQNSETEELGILSLTLRVVFNLFNSIKDHLKVQLEVFFT 367
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig167030.4192.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JJL6_9PHAE6.640e-4684.11SEC7 domain-containing protein n=1 Tax=Ectocarpus ... [more]
D7FVF3_ECTSI1.230e-4584.11GBF1, ArfGEF protein of the BIG/GBF subfamily n=1 ... [more]
A0A2D4CF02_PYTIN1.270e-2659.81SEC7 domain-containing protein (Fragment) n=1 Tax=... [more]
A0A8K1CH01_PYTOL8.210e-2661.22Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A225WXV2_9STRA1.530e-2560.20SEC7 domain-containing protein n=1 Tax=Phytophthor... [more]
A0A6A3JS86_9STRA2.850e-2560.20SEC7 domain-containing protein n=4 Tax=Phytophthor... [more]
A0A7S2WBL3_9STRA2.860e-2552.54Hypothetical protein n=1 Tax=labyrinthulid quahog ... [more]
A0A3F2RJI9_9STRA5.310e-2559.18SEC7 domain-containing protein n=3 Tax=Phytophthor... [more]
W2PX92_PHYPN9.880e-2559.18SEC7 domain-containing protein n=12 Tax=Phytophtho... [more]
A0A3M6VFJ1_9STRA1.350e-2458.16SEC7 domain-containing protein n=1 Tax=Peronospora... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig167030contigH-elongata_contig167030:484..804 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score168.3
Seed ortholog evalue1.9e-39
Seed eggNOG ortholog2880.D7FVF3
Preferred nameGBF1
KEGG koko:K18443
KEGG Pathwayko04144,map04144
GOsGO:0000003,GO:0000137,GO:0000139,GO:0000278,GO:0000281,GO:0000578,GO:0000902,GO:0000910,GO:0000911,GO:0001736,GO:0001738,GO:0001775,GO:0002009,GO:0002064,GO:0002252,GO:0002263,GO:0002376,GO:0002790,GO:0003006,GO:0003674,GO:0005085,GO:0005086,GO:0005488,GO:0005515,GO:0005543,GO:0005547,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005768,GO:0005783,GO:0005793,GO:0005794,GO:0005795,GO:0005798,GO:0005801,GO:0005802,GO:0005829,GO:0005886,GO:0005933,GO:0006810,GO:0006888,GO:0006890,GO:0006891,GO:0006892,GO:0006893,GO:0006895,GO:0006897,GO:0006900,GO:0006901,GO:0006903,GO:0006914,GO:0006928,GO:0006935,GO:0006955,GO:0006996,GO:0007005,GO:0007010,GO:0007029,GO:0007030,GO:0007049,GO:0007155,GO:0007163,GO:0007164,GO:0007275,GO:0007346,GO:0007389,GO:0007424,GO:0007431,GO:0008104,GO:0008150,GO:0008152,GO:0008289,GO:0009056,GO:0009058,GO:0009306,GO:0009605,GO:0009606,GO:0009607,GO:0009615,GO:0009653,GO:0009790,GO:0009791,GO:0009793,GO:0009798,GO:0009826,GO:0009846,GO:0009856,GO:0009880,GO:0009888,GO:0009914,GO:0009926,GO:0009942,GO:0009966,GO:0009987,GO:0010008,GO:0010015,GO:0010053,GO:0010054,GO:0010087,GO:0010101,GO:0010102,GO:0010154,GO:0010256,GO:0010274,GO:0010311,GO:0010540,GO:0010646,GO:0010817,GO:0012505,GO:0015031,GO:0015833,GO:0016020,GO:0016021,GO:0016043,GO:0016049,GO:0016050,GO:0016192,GO:0016197,GO:0016236,GO:0016477,GO:0016482,GO:0017016,GO:0017137,GO:0019898,GO:0019899,GO:0021700,GO:0022402,GO:0022411,GO:0022414,GO:0022610,GO:0022622,GO:0023051,GO:0023052,GO:0030010,GO:0030029,GO:0030036,GO:0030154,GO:0030593,GO:0030595,GO:0030855,GO:0031090,GO:0031224,GO:0031252,GO:0031267,GO:0031410,GO:0031982,GO:0031984,GO:0031985,GO:0032012,GO:0032501,GO:0032502,GO:0032506,GO:0032509,GO:0032879,GO:0032880,GO:0032940,GO:0032989,GO:0033036,GO:0033363,GO:0033365,GO:0034067,GO:0034613,GO:0035091,GO:0035148,GO:0035149,GO:0035150,GO:0035151,GO:0035152,GO:0035158,GO:0035159,GO:0035239,GO:0035272,GO:0035295,GO:0035296,GO:0035964,GO:0040007,GO:0040011,GO:0042147,GO:0042221,GO:0042330,GO:0042802,GO:0042803,GO:0042886,GO:0042995,GO:0043001,GO:0043087,GO:0043167,GO:0043168,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044248,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044432,GO:0044433,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0044706,GO:0045184,GO:0045229,GO:0046578,GO:0046903,GO:0046907,GO:0046983,GO:0048193,GO:0048194,GO:0048199,GO:0048200,GO:0048205,GO:0048209,GO:0048308,GO:0048313,GO:0048316,GO:0048364,GO:0048468,GO:0048469,GO:0048513,GO:0048527,GO:0048528,GO:0048583,GO:0048589,GO:0048608,GO:0048646,GO:0048729,GO:0048731,GO:0048732,GO:0048764,GO:0048765,GO:0048856,GO:0048869,GO:0048870,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0050900,GO:0051020,GO:0051049,GO:0051056,GO:0051179,GO:0051234,GO:0051301,GO:0051336,GO:0051640,GO:0051641,GO:0051648,GO:0051649,GO:0051650,GO:0051656,GO:0051674,GO:0051704,GO:0051707,GO:0051716,GO:0051726,GO:0060326,GO:0060341,GO:0060429,GO:0060541,GO:0060560,GO:0060627,GO:0060918,GO:0061024,GO:0061162,GO:0061339,GO:0061458,GO:0061640,GO:0061919,GO:0061951,GO:0065007,GO:0065008,GO:0065009,GO:0070727,GO:0070887,GO:0070971,GO:0070972,GO:0070973,GO:0071554,GO:0071555,GO:0071621,GO:0071695,GO:0071702,GO:0071705,GO:0071840,GO:0071944,GO:0071985,GO:0072593,GO:0072657,GO:0072659,GO:0080025,GO:0080119,GO:0090066,GO:0090150,GO:0090166,GO:0090406,GO:0090558,GO:0090627,GO:0090696,GO:0090697,GO:0090698,GO:0097111,GO:0097529,GO:0097530,GO:0097708,GO:0098586,GO:0098588,GO:0098657,GO:0098772,GO:0098791,GO:0098805,GO:0098876,GO:0099402,GO:0120025,GO:1901981,GO:1902410,GO:1902531,GO:1902936,GO:1903008,GO:1903047,GO:1903409,GO:1903420,GO:1903827,GO:1905392,GO:1905393,GO:1990266,GO:1990778,GO:2000008
EggNOG free text desc.regulation of ARF protein signal transduction
EggNOG OGsCOG5307@1,KOG0928@2759
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04131
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionconserved unknown protein
Ec32 orthologEc-00_002840.1
Exons1
Model size321
Cds size321
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929975.7283683-CDS-H-elongata_contig167030:483..8041622929975.7283683-CDS-H-elongata_contig167030:483..804Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig167030 484..804 +
1691679231.217142-CDS-H-elongata_contig167030:483..8041691679231.217142-CDS-H-elongata_contig167030:483..804Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig167030 484..804 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig167030.4192.1prot_H-elongata_contig167030.4192.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig167030 484..804 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig167030.4192.1

>prot_H-elongata_contig167030.4192.1 ID=prot_H-elongata_contig167030.4192.1|Name=mRNA_H-elongata_contig167030.4192.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=107bp
GDAEDQNKSVLVRVMRFLSLLSDPRSNGGAEGVLSLSLINIALEAGGSDL
GRIPALVRVMRGDLCKHLLQNSQIEDLNVLSLTLRVVFNLFNSIKDDLKI
QLEVFLT
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mRNA from alignment at H-elongata_contig167030:484..804+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig167030.4192.1 ID=mRNA_H-elongata_contig167030.4192.1|Name=mRNA_H-elongata_contig167030.4192.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=321bp|location=Sequence derived from alignment at H-elongata_contig167030:484..804+ (Himanthalia elongata Himel1 dioecious)
GGAGATGCGGAGGATCAGAACAAGAGCGTTTTGGTGAGGGTGATGCGATT
CCTGTCGCTGCTGAGCGATCCGCGCTCGAACGGCGGTGCCGAGGGCGTCC
TGAGCCTGAGCTTGATCAACATCGCTCTGGAGGCGGGGGGGAGCGACCTC
GGCCGCATCCCCGCCCTCGTGAGGGTGATGCGCGGGGACCTCTGCAAGCA
CCTGCTGCAGAACAGTCAGATCGAGGACCTTAATGTGCTGAGCCTGACCC
TGCGGGTGGTGTTCAACCTGTTTAACTCCATCAAGGACGACCTCAAGATA
CAGCTCGAAGTTTTCCTCACC
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Coding sequence (CDS) from alignment at H-elongata_contig167030:484..804+

>mRNA_H-elongata_contig167030.4192.1 ID=mRNA_H-elongata_contig167030.4192.1|Name=mRNA_H-elongata_contig167030.4192.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=642bp|location=Sequence derived from alignment at H-elongata_contig167030:484..804+ (Himanthalia elongata Himel1 dioecious)
GGAGATGCGGAGGATCAGAACAAGAGCGTTTTGGTGAGGGTGATGCGATT
CCTGTCGCTGCTGAGCGATCCGCGCTCGAACGGCGGTGCCGAGGGCGTCC
TGAGCCTGAGCTTGATCAACATCGCTCTGGAGGCGGGGGGGAGCGACCTC
GGCCGCATCCCCGCCCTCGTGAGGGTGATGCGCGGGGACCTCTGCAAGCA
CCTGCTGCAGAACAGTCAGATCGAGGACCTTAATGTGCTGAGCCTGACCC
TGCGGGTGGTGTTCAACCTGTTTAACTCCATCAAGGACGACCTCAAGATA
CAGCTCGAAGTTTTCCTCACCGGAGATGCGGAGGATCAGAACAAGAGCGT
TTTGGTGAGGGTGATGCGATTCCTGTCGCTGCTGAGCGATCCGCGCTCGA
ACGGCGGTGCCGAGGGCGTCCTGAGCCTGAGCTTGATCAACATCGCTCTG
GAGGCGGGGGGGAGCGACCTCGGCCGCATCCCCGCCCTCGTGAGGGTGAT
GCGCGGGGACCTCTGCAAGCACCTGCTGCAGAACAGTCAGATCGAGGACC
TTAATGTGCTGAGCCTGACCCTGCGGGTGGTGTTCAACCTGTTTAACTCC
ATCAAGGACGACCTCAAGATACAGCTCGAAGTTTTCCTCACC
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