mRNA_H-elongata_contig127835.2050.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig127835.2050.1
Unique NamemRNA_H-elongata_contig127835.2050.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: A0A6H5K6B1_9PHAE (UmuC domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K6B1_9PHAE)

HSP 1 Score: 176 bits (445), Expect = 2.150e-49
Identity = 83/117 (70.94%), Postives = 96/117 (82.05%), Query Frame = 1 
Query:    4 GVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRSKI 354
            GVAHNKLLAKLCSNMHKPNAQTVLP D V  VF  LPV RV+GWGGKFGV+++E LGVSTAGEVA  G + LQR  G ++GWR W+KS  I  +PVK RSAPKS+GCSKTFPG++K+
Sbjct:   60 GVAHNKLLAKLCSNMHKPNAQTVLPLDKVEAVFHTLPVGRVRGWGGKFGVKMMEKLGVSTAGEVAAVGAAELQRVLGDEEGWRAWEKSNAICRDPVKARSAPKSVGCSKTFPGKAKL 176
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: D7FT49_ECTSI (Translesion synthesis DNA polymerase eta splice variant n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FT49_ECTSI)

HSP 1 Score: 176 bits (446), Expect = 5.360e-49
Identity = 83/117 (70.94%), Postives = 96/117 (82.05%), Query Frame = 1 
Query:    4 GVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRSKI 354
            GVAHNKLLAKLCSNMHKPNAQTVLP D V  VF  LPVERV+GWGGKFGV+++E LGVSTAGEVA  G + LQR  G ++GWR W+KS  I  +PV  RSAPKS+GCSKTFPG++K+
Sbjct:  320 GVAHNKLLAKLCSNMHKPNAQTVLPLDKVEVVFHTLPVERVRGWGGKFGVKMMEKLGVSTAGEVAAVGAAELQRVLGDEEGWRAWEKSNAICRDPVNARSAPKSVGCSKTFPGKTKL 436
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: A0A3B3ZQX4_9GOBI (Polymerase (DNA directed), eta n=2 Tax=Periophthalmus magnuspinnatus TaxID=409849 RepID=A0A3B3ZQX4_9GOBI)

HSP 1 Score: 127 bits (319), Expect = 9.850e-32
Identity = 57/118 (48.31%), Postives = 81/118 (68.64%), Query Frame = 1 
Query:    1 TGVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRSKI 354
            +G+AHNK+LAKL   ++KPN QT+LP D VP++F +LP+ +++  GGK G  I ETLG+   GE+     + L + FG + G  ++   RGI+ EPVKPR  PKSIGCSK FPG++ +
Sbjct:  206 SGIAHNKVLAKLACGLNKPNRQTILPMDSVPELFSSLPISKIRNLGGKLGTSITETLGIENMGELTQFSQAQLGQHFGEKTGQWLYDLCRGIEFEPVKPRQLPKSIGCSKNFPGKTSL 323
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: A0A3B3QCM7_9TELE (DNA polymerase eta n=1 Tax=Paramormyrops kingsleyae TaxID=1676925 RepID=A0A3B3QCM7_9TELE)

HSP 1 Score: 126 bits (316), Expect = 2.610e-31
Identity = 58/117 (49.57%), Postives = 80/117 (68.38%), Query Frame = 1 
Query:    4 GVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRSKI 354
            G++HNK+LAKL    +KPN QT+LP   VP++F +LP+ +++  GGK G  IVE+L V   GE+     + L++ FG + G  ++  SRGID EPVKPR  PKSIGCSK FPG+S +
Sbjct:  218 GISHNKVLAKLACGFNKPNRQTILPSGSVPQLFSSLPISKIRNLGGKLGTSIVESLEVENMGEITKFTQAQLEQRFGEKTGQWLFDLSRGIDFEPVKPRQLPKSIGCSKNFPGKSAL 334
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: A0A2I4AR82_9TELE (DNA polymerase eta n=1 Tax=Austrofundulus limnaeus TaxID=52670 RepID=A0A2I4AR82_9TELE)

HSP 1 Score: 125 bits (315), Expect = 3.680e-31
Identity = 59/117 (50.43%), Postives = 80/117 (68.38%), Query Frame = 1 
Query:    4 GVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRSKI 354
            G+AHNK+LAKL   ++KPN QT+LP D V ++F +LPV +++  GGK GV I ETLGV   G++     + L   FG + G  ++   RGI+ EPVKPR  PKSIGCSKTFPG++ +
Sbjct:  228 GIAHNKVLAKLACGLNKPNRQTILPLDSVAELFNSLPVSKIRNLGGKLGVSITETLGVENMGDLTRFSQAELGNHFGEKTGQWLYDLCRGIEFEPVKPRQLPKSIGCSKTFPGKTSL 344
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: A0A3B4D598_PYGNA (DNA polymerase eta n=2 Tax=Pygocentrus nattereri TaxID=42514 RepID=A0A3B4D598_PYGNA)

HSP 1 Score: 125 bits (313), Expect = 6.610e-31
Identity = 58/117 (49.57%), Postives = 80/117 (68.38%), Query Frame = 1 
Query:    4 GVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRSKI 354
            G++HNK+LAKL   ++KPN QT+LP   V ++FR LP+ +++  GGK GV I ETLGV   GE+ +   S L+  FG + G  ++   RGI+ EPVKPR  PKSIGCSK FPG++ +
Sbjct:  209 GISHNKVLAKLACGLNKPNRQTILPLGSVSELFRTLPISKIRNLGGKLGVSITETLGVENMGELTEFTRSQLEENFGEKTGQWLYDLCRGIEFEPVKPRQLPKSIGCSKNFPGKTAL 325
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: A0A1A8NBR1_9TELE (Polymerase (DNA directed), eta (Fragment) n=2 Tax=Nothobranchius rachovii TaxID=451742 RepID=A0A1A8NBR1_9TELE)

HSP 1 Score: 122 bits (307), Expect = 2.260e-30
Identity = 58/117 (49.57%), Postives = 80/117 (68.38%), Query Frame = 1 
Query:    4 GVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRSKI 354
            G++HNK+LAKL   ++KPN QT+LP D V ++F +LPV +++  GGK GV I ETLGV   G++     + L + FG + G  ++   RGI+ EPVKPR  PKSIGCSK FPGR+ +
Sbjct:  245 GISHNKVLAKLACGLNKPNRQTILPLDSVGELFSSLPVSKIRNLGGKLGVSITETLGVENMGDLTRFSRAELGQHFGEKTGQWLFDLCRGIEFEPVKPRQLPKSIGCSKNFPGRTSL 361
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: A0A1A7ZAD2_NOTFU (Polymerase (DNA directed), eta n=7 Tax=Nothobranchius TaxID=28779 RepID=A0A1A7ZAD2_NOTFU)

HSP 1 Score: 123 bits (308), Expect = 3.200e-30
Identity = 58/117 (49.57%), Postives = 80/117 (68.38%), Query Frame = 1 
Query:    4 GVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRSKI 354
            G++HNK+LAKL   ++KPN QT+LP D V ++F +LPV +++  GGK GV I ETLGV   G++     + L + FG + G  ++   RGI+ EPVKPR  PKSIGCSK FPGR+ +
Sbjct:  225 GISHNKVLAKLACGLNKPNRQTILPLDSVGELFSSLPVSKIRNLGGKLGVSITETLGVENMGDLTRFSQAELGQHFGEKTGQWLFDLCRGIEFEPVKPRQLPKSIGCSKNFPGRTSL 341
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: UPI001CF9D4E1 (DNA polymerase eta n=1 Tax=Protopterus annectens TaxID=7888 RepID=UPI001CF9D4E1)

HSP 1 Score: 123 bits (308), Expect = 3.290e-30
Identity = 60/115 (52.17%), Postives = 77/115 (66.96%), Query Frame = 1 
Query:    4 GVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRS 348
            G++HNK+LAKL   ++KPN QT+LP D VP++F  LP+  V+  GGK G  I ETLGV   G++     + LQ  FG + G  ++   RGI+ EPVKPR  PKSIGCSK FPGRS
Sbjct:  230 GISHNKVLAKLACGLNKPNRQTILPLDSVPQLFATLPISSVRNLGGKLGASISETLGVECMGQLTQFSETELQALFGEKTGPWLYALCRGIENEPVKPRQLPKSIGCSKHFPGRS 344
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BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Match: A0A667ZAQ0_9TELE (DNA polymerase eta n=1 Tax=Myripristis murdjan TaxID=586833 RepID=A0A667ZAQ0_9TELE)

HSP 1 Score: 123 bits (308), Expect = 3.370e-30
Identity = 56/117 (47.86%), Postives = 80/117 (68.38%), Query Frame = 1 
Query:    4 GVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFGVRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPRSAPKSIGCSKTFPGRSKI 354
            G++HNK+LAKL   ++KPN QTV+P D VP++F +LP+ +++  GGK G  I ETL V   GE+     + L++ FG + G  ++   RGI+ EPVKPR  PKSIGCSK FPG++ +
Sbjct:  223 GISHNKVLAKLACGLNKPNRQTVVPLDSVPELFNSLPISKIRNLGGKLGASITETLHVENMGELTCYSQAQLEQHFGEKTGQWLYDLCRGIEFEPVKPRQLPKSIGCSKNFPGKTSL 339
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig127835.2050.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5K6B1_9PHAE2.150e-4970.94UmuC domain-containing protein n=1 Tax=Ectocarpus ... [more]
D7FT49_ECTSI5.360e-4970.94Translesion synthesis DNA polymerase eta splice va... [more]
A0A3B3ZQX4_9GOBI9.850e-3248.31Polymerase (DNA directed), eta n=2 Tax=Periophthal... [more]
A0A3B3QCM7_9TELE2.610e-3149.57DNA polymerase eta n=1 Tax=Paramormyrops kingsleya... [more]
A0A2I4AR82_9TELE3.680e-3150.43DNA polymerase eta n=1 Tax=Austrofundulus limnaeus... [more]
A0A3B4D598_PYGNA6.610e-3149.57DNA polymerase eta n=2 Tax=Pygocentrus nattereri T... [more]
A0A1A8NBR1_9TELE2.260e-3049.57Polymerase (DNA directed), eta (Fragment) n=2 Tax=... [more]
A0A1A7ZAD2_NOTFU3.200e-3049.57Polymerase (DNA directed), eta n=7 Tax=Nothobranch... [more]
UPI001CF9D4E13.290e-3052.17DNA polymerase eta n=1 Tax=Protopterus annectens T... [more]
A0A667ZAQ0_9TELE3.370e-3047.86DNA polymerase eta n=1 Tax=Myripristis murdjan Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig127835contigH-elongata_contig127835:725..1532 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score177.6
Seed ortholog evalue3.6e-42
Seed eggNOG ortholog2880.D7FT49
Preferred namePOLH
KEGG koko:K03509,ko:K03510
KEGG Pathwayko01524,ko03460,map01524,map03460
GOsGO:0000003,GO:0000070,GO:0000228,GO:0000278,GO:0000280,GO:0000724,GO:0000725,GO:0000731,GO:0000785,GO:0000790,GO:0000819,GO:0003674,GO:0003676,GO:0003677,GO:0003684,GO:0003824,GO:0003887,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005657,GO:0005694,GO:0005737,GO:0005739,GO:0005829,GO:0006139,GO:0006259,GO:0006281,GO:0006282,GO:0006290,GO:0006301,GO:0006302,GO:0006310,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0007049,GO:0007059,GO:0007062,GO:0007064,GO:0007275,GO:0007346,GO:0008080,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009314,GO:0009411,GO:0009416,GO:0009628,GO:0009650,GO:0009790,GO:0009792,GO:0009987,GO:0010224,GO:0010225,GO:0010564,GO:0010948,GO:0016043,GO:0016407,GO:0016410,GO:0016740,GO:0016746,GO:0016747,GO:0016772,GO:0016779,GO:0018130,GO:0019219,GO:0019222,GO:0019438,GO:0019985,GO:0022402,GO:0031323,GO:0031974,GO:0031981,GO:0032501,GO:0032502,GO:0033554,GO:0034061,GO:0034085,GO:0034086,GO:0034087,GO:0034088,GO:0034641,GO:0034644,GO:0034645,GO:0034654,GO:0035861,GO:0042276,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043596,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044444,GO:0044446,GO:0044454,GO:0044464,GO:0045786,GO:0045787,GO:0045930,GO:0045931,GO:0046483,GO:0048285,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048585,GO:0048856,GO:0050789,GO:0050794,GO:0050896,GO:0051052,GO:0051171,GO:0051276,GO:0051716,GO:0051726,GO:0052858,GO:0060255,GO:0065007,GO:0070013,GO:0070601,GO:0070987,GO:0071214,GO:0071478,GO:0071482,GO:0071494,GO:0071704,GO:0071840,GO:0071897,GO:0071960,GO:0071962,GO:0080090,GO:0080134,GO:0080135,GO:0090304,GO:0090734,GO:0097159,GO:0098813,GO:0104004,GO:0140014,GO:0140097,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1901976,GO:1901977,GO:1903047,GO:1904289,GO:1904290,GO:2000001,GO:2000002,GO:2001020,GO:2001021
EggNOG free text desc.DNA synthesis involved in DNA repair
EggNOG OGsCOG0389@1,KOG2095@2759
EC2.7.7.7
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03400
Hectar predicted targeting categoryother localisation
Exons2
Model size360
Cds size360
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929757.9536192-CDS-H-elongata_contig127835:724..8971622929757.9536192-CDS-H-elongata_contig127835:724..897Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig127835 725..897 -
1691679146.6026542-CDS-H-elongata_contig127835:724..8971691679146.6026542-CDS-H-elongata_contig127835:724..897Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig127835 725..897 -
1622929757.9678335-CDS-H-elongata_contig127835:1345..15321622929757.9678335-CDS-H-elongata_contig127835:1345..1532Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig127835 1346..1532 -
1691679146.6122456-CDS-H-elongata_contig127835:1345..15321691679146.6122456-CDS-H-elongata_contig127835:1345..1532Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig127835 1346..1532 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig127835.2050.1prot_H-elongata_contig127835.2050.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig127835 725..1532 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig127835.2050.1

>prot_H-elongata_contig127835.2050.1 ID=prot_H-elongata_contig127835.2050.1|Name=mRNA_H-elongata_contig127835.2050.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=120bp
TGVAHNKLLAKLCSNMHKPNAQTVLPGDLVPKVFRALPVERVKGWGGKFG
VRIVETLGVSTAGEVADRGPSALQRAFGAQDGWRVWQKSRGIDLEPVKPR
SAPKSIGCSKTFPGRSKIK*
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mRNA from alignment at H-elongata_contig127835:725..1532-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig127835.2050.1 ID=mRNA_H-elongata_contig127835.2050.1|Name=mRNA_H-elongata_contig127835.2050.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=808bp|location=Sequence derived from alignment at H-elongata_contig127835:725..1532- (Himanthalia elongata Himel1 dioecious)
ACAGGGGTGGCCCACAACAAGCTTTTGGCCAAGCTCTGCAGCAACATGCA
CAAGCCCAACGCTCAGACCGTGCTCCCAGGGGACCTTGTTCCCAAGGTCT
TTCGGGCGCTTCCCGTGGAAAGAGTCAAGGGCTGGGGGGGCAAGTTCGGG
GTGCGCATCGTGGAGACACTCGGGGTTTCTACTGCAGGTGTGGCAGAGGG
CGGAGGGGGGGGGGGGAAGAGGAAAGGAGAGCACATACAAAGTTCGTACT
TTGTATTTATTTTCCCTTTTCTGGTAAAAAATGTCTTTCTAGTCTACTCT
ATCTAGTATATTATATTCTAGATCCTAATACTTGGACTGTATAACTTTGA
TACATCTATCGATAAATGCAGGCGTCCTTTCACAGGCACACACGCACACC
CGCAGCTTAGATACGAAATGTAGAGATGGTATGTCTCCTTTGTCACATCG
GATCATTAATATCATTAATAGAGGTTTTTGTAGAAAATATGACTGAATCC
CCCATTGGGAGGATCAATGCGAGAGTATCCTAAAAGGACTAGCATGACAG
GGCCTGATTGCGTGGTTATCTGCAATTTATTAAATACACACATAACTATA
TACACATACTCAAACATATATACACACAAATATAGGCGAGGTGGCCGACA
GAGGACCGAGCGCGCTACAACGAGCGTTCGGCGCGCAGGACGGCTGGAGG
GTGTGGCAAAAGTCGCGGGGGATCGACCTCGAGCCCGTGAAGCCTCGGTC
GGCCCCGAAGAGCATAGGGTGCAGCAAGACGTTCCCCGGGAGGTCGAAGA
TTAAGTGA
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Coding sequence (CDS) from alignment at H-elongata_contig127835:725..1532-

>mRNA_H-elongata_contig127835.2050.1 ID=mRNA_H-elongata_contig127835.2050.1|Name=mRNA_H-elongata_contig127835.2050.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=720bp|location=Sequence derived from alignment at H-elongata_contig127835:725..1532- (Himanthalia elongata Himel1 dioecious)
ACAGGGGTGGCCCACAACAAGCTTTTGGCCAAGCTCTGCAGCAACATGCA
CAAGCCCAACGCTCAGACCGTGCTCCCAGGGGACCTTGTTCCCAAGGTCT
TTCGGGCGCTTCCCGTGGAAAGAGTCAAGGGCTGGGGGGGCAAGTTCGGG
GTGCGCATCGTGGAGACACTCGGGGTTTCTACTGCAGACAGGGGTGGCCC
ACAACAAGCTTTTGGCCAAGCTCTGCAGCAACATGCACAAGCCCAACGCT
CAGACCGTGCTCCCAGGGGACCTTGTTCCCAAGGTCTTTCGGGCGCTTCC
CGTGGAAAGAGTCAAGGGCTGGGGGGGCAAGTTCGGGGTGCGCATCGTGG
AGACACTCGGGGTTTCTACTGCAGGCGAGGTGGCCGACAGAGGACCGAGC
GCGCTACAACGAGCGTTCGGCGCGCAGGACGGCTGGAGGGTGTGGCAAAA
GTCGCGGGGGATCGACCTCGAGCCCGTGAAGCCTCGGTCGGCCCCGAAGA
GCATAGGGTGCAGCAAGACGTTCCCCGGGAGGTCGAAGATTAAGTGAGCG
AGGTGGCCGACAGAGGACCGAGCGCGCTACAACGAGCGTTCGGCGCGCAG
GACGGCTGGAGGGTGTGGCAAAAGTCGCGGGGGATCGACCTCGAGCCCGT
GAAGCCTCGGTCGGCCCCGAAGAGCATAGGGTGCAGCAAGACGTTCCCCG
GGAGGTCGAAGATTAAGTGA
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