prot_H-akashiwo_Contig917.1.3 (polypeptide) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: A0A7S2UT03_9STRA (Phenylalanine--tRNA ligase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UT03_9STRA)

HSP 1 Score: 626 bits (1615), Expect = 7.220e-220
Identity = 332/528 (62.88%), Postives = 380/528 (71.97%), Query Frame = 0

Query:   19 LRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGAFNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEYRAGTAVLGQPCS-GPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVAGSGDAIVVTVPPTRSDILHAVDVIEDVAIAYGQRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLRRPGAPAVRLSNPAIIEYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVLPDPAHPIGARNERRLSAAYVGLTAGFEVIHGLVDRVMALVQVAPDPAYAGNSMR---------------DLAATVAPRKGVCYKVVPGSDPAFFEGRCAEVMLVREGEGGQQESPVGVFGVVHPEVLANFDLVNPCSALELNIEALM 530
            LR VT DP+RYKS IDLQDKLHQNICR R  VAIGTHDL T++GPFRYE   P DI FVPL P DR  F A+ L+DHY TD DCKHLK YV LI+DAPAYPVIYD+ D V+SLPP+INGDHSKI L T+N+FIECTATD TKANIVLDTMVTMFSQYC +PFTVE V+V Y  G A   +      TP+L+ R E AKV +VNST GI+I+AEEMCRLCTKMQLGPA      DAI VTVPPTR+DILHAVDVIEDVAIAYG      T P            L VG PQP+N F DLLREEI+RAGY EMLTHGLCS +ENF  L +P  PAV L NPA +EYEVVRT LLPG LKTVQHNR+MP + G++LFEISD+V  DP H IGA N+R L A YVGLTAGFEVIHGLVDRVMAL QVAP   Y  +S++                   TVAPR GV Y + P  +P FF GRCA+++L        Q   +G FG++HP+VL ++DL NPCS LE+ +EAL+
Sbjct:    4 LRGVTLDPKRYKSLIDLQDKLHQNICRMRSLVAIGTHDLDTIQGPFRYECLPPQDIEFVPLTPNDR-LFKAKDLMDHYNTDKDCKHLKPYVPLIYDAPAYPVIYDSNDVVLSLPPLINGDHSKIELHTKNIFIECTATDLTKANIVLDTMVTMFSQYCTNPFTVEEVEVVYEEGHAHASKKEKVAVTPVLTNRTEVAKVKDVNSTAGINITAEEMCRLCTKMQLGPATYDQELDAICVTVPPTRADILHAVDVIEDVAIAYGYNNIITTVPNT----------LTVGGPQPMNLFCDLLREEISRAGYTEMLTHGLCSKQENFGHLLQPETPAVELLNPANLEYEVVRTTLLPGALKTVQHNRSMPTKDGIKLFEISDIVQVDPDHAIGACNKRHLVAVYVGLTAGFEVIHGLVDRVMALCQVAPAADYVSSSLKKPTNKGAGTTSGDQVSAVTTVAPRAGVEYSIQPAQNPTFFPGRCADILLH---SNESQIIKLGTFGILHPKVLQSYDLSNPCSVLEMELEALV 517          

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: D8LJA1_ECTSI (Phenylalanine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LJA1_ECTSI)

HSP 1 Score: 584 bits (1506), Expect = 1.610e-201
Identity = 318/541 (58.78%), Postives = 380/541 (70.24%), Query Frame = 0

Query:    1 MSTALAGGRLRPSGVLRVLRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGAFNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEYRAGTAVLGQPCSGPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVAGSGD-----AIVVTVPPTRSDILHAVDVIEDVAIAYGQRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLRRPGAPAVRLSNPAIIEYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVLPDPAHPIGARNERRLSAAYVGLTAGFEVIHGLVDRVMALVQVAPDPAYAGNSMRDLAATVAP-RKGVCYKVVPGSDPAFFEGRCAEVMLVRE-----GEGGQQESPVGVFGVVHPEVLANFDLVNPCSALELNIEALM 530
            M+      ++RP  V  VLR VTF+ + YKSF+DLQDKLHQNICR+R  VAIG HDL TV+GPFRY+   P DI FVPL P+D+G FNA+ALLD Y TDP  KHLK Y  +I+++  YPVI DA   V+SLPP+ING  S+I L+T +VFIECTATD TKANIVLDTMVTMFS+YC   F+VEPVDV Y   +  +       TPLLSTR E A V E+ S IG++I  EE+CRLCTKMQLGPA V   G+      I VTVPPTRSDILH VDV+EDVAIAYG    P T P          A L VG  QP+N+F DLLREEI+RAGY EMLTHGLCS  ENF +LRR   P V LSNP  +EYEVVRT LLPG LKT Q NRAMPV+ GVRLFEISDVV+ D +   GARN RRL A Y+G TAGFE+IHGLVDR+M+LVQVAP   Y+GN  R  A    P R+ + Y++ P   P FF GRCA+V+L+ +     G  G +E  +G FG++HPEVL  +DL +P SA+E+++E LM
Sbjct:  115 MTVGSGAAKIRPYVVCAVLRGVTFNSKSYKSFMDLQDKLHQNICRRRTLVAIGAHDLNTVKGPFRYDAVPPSDIDFVPLTPSDQGTFNAKALLDLYNTDPSVKHLKPYTPIIYESEVYPVIKDANGDVLSLPPVINGHLSRIRLETTDVFIECTATDLTKANIVLDTMVTMFSEYCGDKFSVEPVDVTYDDNSGDVKT-----TPLLSTRTEKASVAEICSIIGVEIEEEEICRLCTKMQLGPAEVVEMGEEEGGKGIKVTVPPTRSDILHPVDVVEDVAIAYGFNKIPTTLP----------ATLTVGGHQPLNNFGDLLREEISRAGYTEMLTHGLCSRAENFKMLRRQEEPCVSLSNPKHVEYEVVRTSLLPGALKTAQFNRAMPVKDGVRLFEISDVVVRDDSCDTGARNCRRLVATYMGPTAGFEIIHGLVDRIMSLVQVAPSEEYSGNDPR--ARVGGPWRENMRYRIDPVDRPTFFPGRCADVLLMVKDTEEGGSAGWREIRLGSFGMLHPEVLKAYDLRDPSSAVEIDLEPLM 638          

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: W7TXC3_9STRA (Phenylalanine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TXC3_9STRA)

HSP 1 Score: 574 bits (1480), Expect = 8.960e-198
Identity = 306/532 (57.52%), Postives = 368/532 (69.17%), Query Frame = 0

Query:    1 MSTALAGGRLRPSGVLRVLRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGAFNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEYRAGTAVLGQPCSGPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVAGSGDAIVVTVPPTRSDILHAVDVIEDVAIAYGQRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLRRPGAPAVRLSNPAIIEYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVLPDPAHPIGARNERRLSAAYVGLTAGFEVIHGLVDRVMALVQVAPDPAYAGNSMRDLAATVAPRKGVCYKVVPGSDPAFFEGRCAEVMLVREGEGGQQESP--VGVFGVVHPEVLANFDLVNPCSALELNIEALM 530
            MS   +   +RP     +LR +TFDP  YKSFIDLQ+ LH+NICR+R  VAIGTHDL T+RGPFRY    P  I F PL   D   FNA+ LLD Y TD   KHL  Y  +I++AP YPV+YD++  V+SLPP+ING HS+I+L TRNVFIECTATD TKANIVLDT+VTMFSQ+CA PFTVEPVDV Y      +       TPLLSTR+  AK+ E+   +G D+  E +C+LC KMQLGPA      D +VVTVPPTRSD+LHAVDVIEDVAIA+G    P      ++ PG     L VG P PIN F+DLLR+EIARAGY+E+LTHGLCS  ENFA LRRP   AV LSNPA  EYE+VRT LLPG LK +QHNRA  V+ G+R FEISDVVL D    +GA+N RRL A+   + AGFE IHGLVDRVM LVQ+ P  AYAGNSMR        +  + Y +VP  DPA+F GRCA+V+LV   E G++  P  VG FGV+HPEVL  +D+  P S LE+++E L+
Sbjct:  112 MSVEASTASVRPFVACAILRDMTFDPLVYKSFIDLQEHLHRNICRRRTLVAIGTHDLDTLRGPFRYTAADPASIQFSPLTSDDGRVFNAKELLDFYRTDESAKHLSPYTDIIYEAPRYPVLYDSRGTVLSLPPVINGRHSRITLQTRNVFIECTATDKTKANIVLDTVVTMFSQHCARPFTVEPVDVVYEGPELSVET-----TPLLSTRKVAAKMSEIEGLVGRDLGPELVCQLCNKMQLGPASYHAETDTVVVTVPPTRSDVLHAVDVIEDVAIAFGYNNLP------IKVPGT----LTVGAPLPINAFSDLLRDEIARAGYLEVLTHGLCSRAENFAKLRRPETKAVSLSNPANEEYEIVRTTLLPGLLKVLQHNRAAQVKDGLRFFEISDVVLRDDHTDVGAKNIRRLVASSTSMVAGFESIHGLVDRVMLLVQIGPTEAYAGNSMRGEERMAVLKDKIQYYIVPSDDPAYFPGRCADVILV---EDGKKSKPLKVGTFGVLHPEVLKAYDIPFPTSMLEIDLEPLI 625          

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: A0A835YVN2_9STRA (Phenylalanine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YVN2_9STRA)

HSP 1 Score: 541 bits (1393), Expect = 1.640e-184
Identity = 303/543 (55.80%), Postives = 358/543 (65.93%), Query Frame = 0

Query:    9 RLRPSGVLRVLRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGAFNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEYRA--GTAVLGQPCSGPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVAGSGDAIVVTVPPTRSDILHAVDVIEDVAIAYGQRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLR----RPGAPAVRLSNPAII----EYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVLPDPAHPIGARNERRLSAAYVGLTAGFEVIHGLVDRVMALVQVAPDPAYAGNSMRDLAATVAP----RKGVCYKVVPGSDPAFFEGRCAEVMLVREGE------GGQQESPVGVFGVVHPEVLANFDLVN-PCSALELNIEALM 530
            ++RP  V  VLR VTFD R YKSFIDLQDKLHQNICR+R  VAIGTHDL T+ GPF YE   P DI FVPLVP DRGAF A+ALLD Y TD   KHLK Y G+I+D+P YPV+ DA+  V SLPP+ING HS+I   TRNVFIECTATD TKANIVLDT+V MFSQ CA PFT EPVDV Y A   TA         TPLLS+R     + ++   +G+D++ EE CRLC KMQLGPA V   G AI VT PPTRSD+LHAVDV+EDVAIA+G    P   PR             VG PQP+    DLLR E+ARAGY E+LTHGLC   +  A+ R      G  A  L   A+     +YEVVRT LLPG LKT+QHNR++ V+ G+RLFEISDVVL DP    GARN RRL A Y G TAGFE++HGLVDRVM L+QV P  +YAG S+   A         R+G+ Y V P  +  FF GRCA+V+L R G        G +E  +G FGV+HPEVL  +++   P S LE+++E LM
Sbjct:  108 QIRPYVVCAVLRGVTFDARTYKSFIDLQDKLHQNICRRRTLVAIGTHDLDTLEGPFTYEALPPQDIEFVPLVPCDRGAFKAKALLDLYNTDVAVKHLKPYTGIIYDSPVYPVVLDARRTVCSLPPVINGYHSRIQQTTRNVFIECTATDLTKANIVLDTVVAMFSQLCADPFTAEPVDVVYEATGNTAT--------TPLLSSRTLEVAMADIRGILGVDMAPEEACRLCAKMQLGPATVTQGGAAIAVTAPPTRSDVLHAVDVVEDVAIAHGYNALPLRAPRA----------HTVGAPQPLAAVCDLLRAEVARAGYAELLTHGLCDRRDLVAVARCEGGEEGPQAAALLGQAVALGDADYEVVRTTLLPGVLKTLQHNRSLSVKDGLRLFEISDVVLQDPTTDTGARNVRRLCALYTGNTAGFEIVHGLVDRVMQLLQVVPAASYAGASVSKAAGGAIEGGPWREGLEYFVRPVDNRTFFPGRCADVVLRRSGXXXXXXXSGAEEVRLGSFGVLHPEVLHAYEVGEYPASVLEMDLEPLM 632          

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: B7G0G3_PHATC (Phenylalanine--tRNA ligase n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7G0G3_PHATC)

HSP 1 Score: 540 bits (1390), Expect = 6.050e-184
Identity = 294/537 (54.75%), Postives = 371/537 (69.09%), Query Frame = 0

Query:    9 RLRPSGVLRVLRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGAFNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEYRAGTAVLGQPCSGPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVAGSGDAIVVTVPPTRSDILHAVDVIEDVAIAYG----QRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLRRPGAPAVRLSNPAIIEYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVLPDPAHP-----IGARNERRLSAAYVGLTAGFEVIHGLVDRVMALVQVAPDPAYAGNSMR-DLAATVAPRKGVCYKV--VPGSDP---AFFEGRCAEVMLVREGEGGQQESPVGVFGVVHPEVLANFDLVNPCSALELNIEALM 530
            ++RP  V  +LR VTFD  RY SFIDLQD+LH+N+CRQR  VAIGTHD+  V+GPF Y+ +AP DISFVPL P D+ ++ A  LL+ Y T+P  KHLK YV +I D+P YPVI D+ + V+SLPPIING  SKI+LDT+NVFIECTATD TKANIVLDTMVTMFS YC  PFTVEPV V+Y+    ++    S  TP+LS+R+ETAKVD  NS IGI I+ +EM  LC K+QLGPA++  +   + VTVPPTRSDILH VD+ ED+ IAYG    +R  P T                VG   P+N   DLLREEI RAGY+E+LTHGLCS  +NF  L+RP   AV+LSNPA +EY+VVRT LLPG LKT+QHN++     G +LFEISDVVLPD  +      +GA+N RRL+A Y G T+GFE+IHGLVDRVM L+++AP+ AY  NS + D       R+G  Y +  +  SDP    +F GR A ++L +          VG FG++HP+VLANFD++ P S +EL++EALM
Sbjct:  124 QIRPFVVCAILRNVTFDEERYASFIDLQDQLHRNLCRQRTLVAIGTHDMDVVQGPFFYDARAPTDISFVPLTPNDK-SYVANELLEFYETEPAAKHLKPYVPIIKDSPLYPVIVDSNETVLSLPPIINGSKSKITLDTKNVFIECTATDLTKANIVLDTMVTMFSGYCEVPFTVEPVLVQYQENDKIVS---SYVTPVLSSRQETAKVDFCNSLIGIQITPQEMQALCNKVQLGPAQLLENDTVLQVTVPPTRSDILHPVDIAEDIGIAYGYNNIRRRVPTT--------------NTVGGEFPLNQTGDLLREEIGRAGYVEVLTHGLCSVRDNFTALQRPVTAAVKLSNPANVEYQVVRTTLLPGLLKTLQHNKSASFTGGFKLFEISDVVLPDSENVMTETIVGAKNSRRLAAVYAGPTSGFEIIHGLVDRVMTLLEIAPEEAYIANSAKSDEEEYRVAREGWTYTIASLSESDPHVNTYFPGRAAAILLTKP--NATSSCVVGTFGILHPDVLANFDILYPSSCVELDLEALM 640          

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: K0SRH2_THAOC (Phenylalanine--tRNA ligase n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0SRH2_THAOC)

HSP 1 Score: 539 bits (1389), Expect = 8.020e-184
Identity = 297/532 (55.83%), Postives = 367/532 (68.98%), Query Frame = 0

Query:   10 LRPSGVLRVLRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGA-FNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEYRAGTAVLGQPCSGPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVAGSG----DAIVVTVPPTRSDILHAVDVIEDVAIAYGQRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLRRPGAPAVRLSNPAIIEYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVLPDPA-----HPIGARNERRLSAAYVGLTAGFEVIHGLVDRVMALVQVAPDPAYAGNSMRDLAATVAPRKGVCYKVVP-GSDPAFFEGRCAEVMLVREGEGGQQESPVGVFGVVHPEVLANFDLVNPCSALELNIEALM 530
            +RP  V  VLR VTFDP RY+SFI+LQD+LH+N+CRQR  VAIGTHDL +VRGPF Y+ +APG+I FVPL   D G  F+ R L++HY T+P CKHLK YV +I D+P YPV+ D++  V+SLPPIING  S+I+LDT+NVFIECTATD TKANIVLDT+V MFS+Y A PFTVEPV V Y    A  G   S  TP + TR+ETAKVD VNS IGI + + +M  LC ++QLGPAR+   G    D + VTVPPTRSDILHAVDV EDV IA+G      T P              VG  QP+N   DLLR+EI RAGY E+LTHGLCST +NF  L RP   AV+LSNPA +EYEVVRT LLPG LKT+QHN++M    G +LFEISDVVLPD         +GARN RR+ A + G ++GFE+IHGLVDR+M L +VAP+  Y  +S      +V  ++G  Y +     D ++F GR AEV+L R   GG  E P G FG++HP+VL  FD++ P SA+ELN+E L+
Sbjct:  123 VRPYVVCAVLRDVTFDPERYQSFIELQDQLHRNLCRQRTLVAIGTHDLDSVRGPFTYDARAPGEIKFVPLTHTDEGREFDGRELMEHYETEPACKHLKPYVPIIKDSPLYPVVLDSEGTVLSLPPIINGSKSRITLDTKNVFIECTATDLTKANIVLDTVVAMFSEYAARPFTVEPVTVSYV--DAAGGVARSYVTPQMYTRKETAKVDFVNSLIGISVESSKMADLCNRIQLGPARIIPDGQTGEDVLEVTVPPTRSDILHAVDVAEDVGIAHGYNNIVRTVP----------GTCTVGREQPLNQLGDLLRDEIGRAGYTEVLTHGLCSTADNFTALGRPVTAAVKLSNPANVEYEVVRTTLLPGLLKTLQHNKSMSFAGGFKLFEISDVVLPDSECVVSETVVGARNSRRVCATFTGPSSGFEIIHGLVDRIMTLCEVAPEAGYVASS-GSAYKSVYCKEGWEYTIRELRGDGSYFPGRGAEVLLKRP--GGDFE-PAGSFGILHPDVLRAFDVLYPTSAVELNLEKLL 638          

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: A0A1Z5JYF5_FISSO (Phenylalanine--tRNA ligase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JYF5_FISSO)

HSP 1 Score: 529 bits (1362), Expect = 5.010e-180
Identity = 288/529 (54.44%), Postives = 358/529 (67.67%), Query Frame = 0

Query:   10 LRPSGVLRVLRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGAFNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEY--RAGTAVLGQPCSGPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVAGSGDAIVVTVPPTRSDILHAVDVIEDVAIAYGQRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLRRPGAPAVRLSNPAIIEYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVLPDPAHPI-----GARNERRLSAAYVGLTAGFEVIHGLVDRVMALVQVAPD-PAYAGNSMRDLAATVAPRKGVCYKVVPGSDPAFFEGRCAEVMLVREGEGGQQESPVGVFGVVHPEVLANFDLVNPCSALELNIEALM 530
            +RP  V  +LR + FD +RY SFI LQD+LH+N+CRQR  VAIGTHDL   + PF Y+ +AP  I FVPL PA R  F A+ LL+ Y T  + KHLK YV +I ++P YPVI DA + V+SLPPIINGDHSKI+LDT NVFIECTATD TKANIVLDT+VTMFS+YCA+PF VEPV V Y   +GT V     S  TPLL TR+E A VD  NS IGI +S +EM +LC K+QLGPA +      + VTVPPTRSDILHAVD+ ED+ IA+G        P+             VG+  P+N  TDLLR+EIARAGY E+LTHGLCS E+NF  LRRP APAV L NPA +EY+VVRT LLPG L T+QHNRA     G +LFEISDVV+PD  H +     GA+N+R + A Y G T+GFE+IHGL DR+M L +VAP+ P  A    R +      R+G  Y + P   P +F GR A+++L +    G   + +G FG++HPEVL +F L+ P S LE+++EALM
Sbjct:  114 IRPFVVCAILRNIQFDAQRYDSFIALQDQLHRNLCRQRTLVAIGTHDLDATQPPFFYDARAPSSIEFVPLTPATR-KFEAKELLEFYETQTEGKHLKPYVPIIKESPLYPVILDAAETVLSLPPIINGDHSKITLDTTNVFIECTATDLTKANIVLDTVVTMFSEYCATPFQVEPVVVNYVDPSGTVV----SSYTTPLLYTRQEQASVDFCNSLIGIQLSPQEMQQLCEKVQLGPANLLEKDTMLQVTVPPTRSDILHAVDIAEDIGIAFGYNNIVKRVPKTA----------TVGQEFPLNLLTDLLRDEIARAGYTEVLTHGLCSLEDNFTALRRPIAPAVHLLNPANVEYQVVRTTLLPGLLNTLQHNRATSFTNGFKLFEISDVVVPDNEHVVTETIVGAKNQRMVCAVYAGPTSGFEIIHGLADRIMTLTEVAPELPDDADKDHRVV------REGWSYAIEPCDAPTYFSGRAAQIVLTKP---GSSPTVIGSFGILHPEVLQHFSLLYPASCLEIDLEALM 618          

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: A0A448Z5X7_9STRA (Phenylalanine--tRNA ligase n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448Z5X7_9STRA)

HSP 1 Score: 529 bits (1362), Expect = 1.420e-179
Identity = 288/543 (53.04%), Postives = 362/543 (66.67%), Query Frame = 0

Query:   10 LRPSGVLRVLRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGAFNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEYRAGTAVLGQPCSGPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVAGSGDAIVVTVPPTRSDILHAVDVIEDVAIAYGQRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLRRPGAP--AVRLSNPAIIEYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVLPDPAHPI-----GARNERRLSAAYVGLTAGFEVIHGLVDRVMALVQVAPDPAYAGNSMRDLAATVAP--------RKGVCYKVVP-------GSDPAFFEGRCAEVMLVREGEGGQQESPVGVFGVVHPEVLANFDLVNPCSALELNIEALM 530
            +RP  V  +L+ +TF   RY SFIDLQD+LH+N+CRQR  VAIGTHDL +V GPF Y+ + P DI FVPL P+D+G+F    L+++Y T+   KHLK YV +I D+P YPVI DAQDRV+SLPPIINGDHSKI+LDT+NVFIECTATD TKANIVLDT++TMFS+YCA+PFTV PV VEY   +  +    S  TP LS R E AK+D  NS IG+++SA E+  LC K+QLGPA +  +   + VTVPPTRSDILHAVD+ ED+AIAYG        P+             VG  +P+N   DLLREEI RAGY E+LTHGLCS  +NF  LR P     AV LSNPA IEYEV RT LLPG LKT QHN++     G +LFEISD VL D  + +     GARN R+L+AAY G T+GFE+IHGLVDRVM L++VAP+ +Y   S +  +AT           R+G  Y + P            +F GR A+++L +      ++  VG FG++HP VL NFD+  PCS +E+N+E LM
Sbjct:  122 IRPFVVAAILKDITFTQERYDSFIDLQDQLHRNLCRQRTLVAIGTHDLDSVEGPFVYDARPPADIDFVPLTPSDKGSFKGGDLMNYYETEVSAKHLKPYVPIIKDSPLYPVIVDAQDRVLSLPPIINGDHSKITLDTKNVFIECTATDLTKANIVLDTVLTMFSEYCAAPFTVVPVQVEYHDDSGSVVD--SYLTPKLSVRREQAKIDFCNSLIGVNLSAAEIQTLCNKVQLGPANLLENDTLLEVTVPPTRSDILHAVDIAEDIAIAYGYNNIVKRVPKT----------NTVGGAEPLNQVGDLLREEIGRAGYTEVLTHGLCSIHDNFTALRCPVREDWAVSLSNPANIEYEVCRTTLLPGLLKTFQHNKSASFANGFKLFEISDAVLVDRENVVTDTIVGARNVRKLAAAYAGPTSGFEIIHGLVDRVMTLIEVAPEASYVATSGKKSSATGGADEEMYRISREGWFYTIAPLDYDGDAHEAKMYFPGRAAQILLTKP--NCSEKIRVGTFGILHPLVLKNFDIQYPCSVVEINLEELM 650          

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: A0A7S1U5G5_9STRA (Phenylalanine--tRNA ligase n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1U5G5_9STRA)

HSP 1 Score: 528 bits (1359), Expect = 2.420e-178
Identity = 306/567 (53.97%), Postives = 365/567 (64.37%), Query Frame = 0

Query:   10 LRPSGVLRVLRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGAFNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEYRAGTAVLGQPCSGPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVA--------GSGDAIVVTVPPTRSDILHAVDVIEDVAIAYGQRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLRRPGAPA----VRLSNPAIIEYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVL-------PDPAHP-----IGARNERRLSAAYVGLT-AGFEVIHGLVDRVMALVQVAPDPAYAGNSMRDLA----------------ATVAPRKGVCYKVVPGSDPAFFEGRCAEVMLVREG--EGGQQESPVGVFGVVHPEVLANFDLVN----PCSALELNIEAL 529
            +RP  V  VLR VTF P+RY+SFI+LQDKLHQNICR+R  VAIGTHDL T+  PFRY    P DI+FVPL   +R +F+ +ALLDHY TD  CKHL  YV +I+D P YPVIYDA+DRV+SLPPIING HS+I LDTR+VFIECTATD+TKANIVLDT+VTMFS++C  PF+VE VDV Y A    +       TP ++ R + A V +VNS +GIDI+ E MC LC KMQLGPA           G G  I VTVPPTR DI+H+VDVIED+ +AYG      T P            L VG PQP+NHF DLLR+EIARAG+ E LTHGLCS  EN+ +LRR    A    V LSNPA  EYE+VRT LLPG LKT+Q NR   +   +++FEISDVV        P  + P     +GARN RRLSA Y  +  +GFEVIHGLV RVM +VQ+ P       S +                    T+APR G+ Y+V  G DP FF GRCAEV L R G  +    E  VG+FGVVHPEVLAN+ LV     P S LEL++E L
Sbjct:  153 VRPHVVCAVLRGVTFTPKRYQSFIELQDKLHQNICRRRTLVAIGTHDLATLTPPFRYRALPPTDINFVPLTEEER-SFDGKALLDHYKTDGQCKHLAPYVDIIYDEPNYPVIYDAEDRVLSLPPIINGRHSRIRLDTRDVFIECTATDYTKANIVLDTVVTMFSEHCDKPFSVEKVDVIYEAEGGRVDT-----TPKMTVRSDQAAVADVNSLVGIDITPERMCELCDKMQLGPATHVPEGPDSGPGKGGLIRVTVPPTRHDIMHSVDVIEDIGVAYGFNNIVKTVPET----------LTVGAPQPLNHFCDLLRDEIARAGFSETLTHGLCSIRENYEMLRRDSEAAATEYVSLSNPANEEYEIVRTSLLPGMLKTLQANRGTALSVPLKIFEISDVVFRDLEFTTPSVSGPYDLGVVGARNARRLSALYASMQGSGFEVIHGLVTRVMQVVQL-PHLLQVRESAKTTTDGEEEDKKWSMIPPGGQTLAPRPGLAYRVAAGDDPCFFPGRCAEVFLCRLGADDAVLSEERVGLFGVVHPEVLANYGLVKIHAYPTSVLELDLEPL 702          

BLAST of mRNA_H-akashiwo_Contig917.1.3 vs. uniprot
Match: A0A7S2H2G7_9STRA (Phenylalanine--tRNA ligase n=1 Tax=Helicotheca tamesis TaxID=374047 RepID=A0A7S2H2G7_9STRA)

HSP 1 Score: 525 bits (1353), Expect = 2.770e-178
Identity = 290/539 (53.80%), Postives = 359/539 (66.60%), Query Frame = 0

Query:   10 LRPSGVLRVLRLVTFDPRRYKSFIDLQDKLHQNICRQRKYVAIGTHDLGTVRGPFRYEHKAPGDISFVPLVPADRGAFNARALLDHYLTDPDCKHLKDYVGLIHDAPAYPVIYDAQDRVMSLPPIINGDHSKISLDTRNVFIECTATDFTKANIVLDTMVTMFSQYCASPFTVEPVDVEYRAGTAVLGQPCSGPTPLLSTREETAKVDEVNSTIGIDISAEEMCRLCTKMQLGPARVAGSGDAIVVTVPPTRSDILHAVDVIEDVAIAYGQRYAPHTPPRQVQQPGAGGAPLCVGEPQPINHFTDLLREEIARAGYIEMLTHGLCSTEENFALLRRPGAP--AVRLSNPAIIEYEVVRTVLLPGTLKTVQHNRAMPVRQGVRLFEISDVVLPDPAHPI-----GARNERRLSAAYVGLTAGFEVIHGLVDRVMALVQVAPDPAY------AGNSMRDLAATVAPRKGVCYKVVPGSDP-----AFFEGRCAEVMLVREGEGGQQESPVGVFGVVHPEVLANFDLVNPCSALELNIEALM 530
            +RP  V  +LR VTF P RY SF+DLQD+LH+N+CRQR  VAIGTHDL +V+GPF Y+ + P +I+FVPL P     FN++ALL+ Y TD +CK LK YV LI D+P YPV+ D+ D VMSLPPIINGDHSKI+LDT+ VFIECTATD TKANIVLDTMVTMF+QYCA P+ +EPV V Y+     +    S  TP LSTR+ETA+V  VNS IGI++ A +M  LC K+QLGPA++  S   + VTVPPTRSDILH VD+ ED+AIAYG        P+             VG  QP+N  TDLLR+EI RAGY E+LTHGLCS  +NF  L R      AV LSNPA IEYEVVRT LLPG +KT+QHNR+     G++LFEISDVVL D  H I     GARN RR+ A Y G T+GFE++HGLVDR+M L +VAP   Y      AG SM +       R+G  Y+V    +       +F GR A  M+V    G + E  +G FG++HP+VL NFD+  P S +EL++  L+
Sbjct:  121 IRPFVVCAILRDVTFTPERYASFLDLQDQLHRNLCRQRSLVAIGTHDLDSVQGPFTYDARKPEEINFVPLKPDGSPTFNSKALLELYETDAECKSLKPYVPLIKDSPLYPVVLDSNDVVMSLPPIINGDHSKITLDTKQVFIECTATDLTKANIVLDTMVTMFAQYCAKPYEIEPVKVTYKNANGEITN--SYTTPTLSTRKETAQVSFVNSLIGIELEATKMLTLCNKLQLGPAQLLDSNTTLEVTVPPTRSDILHPVDIAEDIAIAYGYNNIVKRVPQT----------CTVGGEQPLNKLTDLLRDEIGRAGYTEVLTHGLCSLHDNFTALGREEKKEEAVSLSNPANIEYEVVRTTLLPGLMKTLQHNRSASFSNGMKLFEISDVVLADGKHAITETVVGARNVRRVCAIYAGPTSGFEIVHGLVDRIMTLTEVAPGGEYIKATQGAGGSMGEEEQFRVAREGWEYEVRALEESRDEIGTYFPGRAA--MVVVRNPGVEGEVVLGTFGILHPKVLKNFDVSYPASCVELDLSPLL 645