mRNA_H-akashiwo_Contig940.6.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig940.6.1
Unique NamemRNA_H-akashiwo_Contig940.6.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: A0A482SNF4_9ARCH (Fatty acid hydroxylase family protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SNF4_9ARCH)

HSP 1 Score: 181 bits (459), Expect = 1.170e-53
Identity = 84/182 (46.15%), Postives = 119/182 (65.38%), Query Frame = 3
Query:   18 NLSVATLFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTYFGQYCGMEFCPPR 563
            NL +A+ F     E  VR    +T+++F +    L  +F  ++ A  ++S+ EYYWH LMH    Y  FHK HH YK P PFDDMYIHP+E+FGYYCI +AP  +  +  VSF  Y+VI+G  GV+DHSG  F +P +YN+ DH++HH  F VNY+FPFP++D+LH T+ G++ G ++   R
Sbjct:   76 NLFIASCFALMATECSVRRVNRMTYEDFDVNL--LPTIFCQVLLACTYESLAEYYWHRLMHLKFFYIAFHKMHHQYKAPEPFDDMYIHPVEAFGYYCILYAPPFLFTMHYVSFIAYMVIMGIFGVMDHSGIKFSIPAIYNTVDHDSHHAKFEVNYSFPFPYLDILHGTFEGEFLGRQYTRKR 255          
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: L1JX35_GUITC (Sterol desaturase n=2 Tax=Guillardia theta TaxID=55529 RepID=L1JX35_GUITC)

HSP 1 Score: 166 bits (421), Expect = 5.860e-48
Identity = 85/179 (47.49%), Postives = 111/179 (62.01%), Query Frame = 3
Query:   48 FTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTYFGQYCGMEFCPPRPSVKQKM 584
            F V  IVRG T L FD     A  L  V      A+ +QS+ EYYWH LMH    YK FHK HH YK P+PFDDMYIHP+E+ GYYCI ++P  V  +    F LY+ I+G CG++DHSG  +    +YN++DH+ HH  F+ N+AFPF +MD+LH T+ G + G+ +  P  S K  M
Sbjct:   90 FVVRSIVRGSTRLLFD--WKAAGRLPGVVGTFCLAVGWQSVLEYYWHRLMHLPFFYKHFHKMHHSYKSPQPFDDMYIHPVEAVGYYCILYSPPFVFPLHVYGFVLYMAIMGVCGILDHSGIKWGFLGIYNTEDHDKHHEHFDCNFAFPFVWMDILHGTFAGDHMGIHY--PLSSAKLGM 264          
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: A0A6G0WDA8_9STRA (Fatty acid hydroxylase domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WDA8_9STRA)

HSP 1 Score: 165 bits (418), Expect = 1.120e-47
Identity = 79/181 (43.65%), Postives = 114/181 (62.98%), Query Frame = 3
Query:   18 NLSVATLFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTYFGQYCGMEFCPP 560
            NL +A      + E  +RG + +TFD     A  +R++    + A VF++  EYYWH L+H   +Y R HK HHHYK P+PFDDMY+HP E   YY + + P  V  +   +F++Y++I+GT GV+DHSG  F++PL+YNS+DH  HH    VN  FPFP+ D+LH T+ G++ G+ F  P
Sbjct:   71 NLLLAATCAMVSTELHMRGHSQMTFDASPSIATIVRQI----IVACVFENTVEYYWHCLLHCRFLYTRVHKVHHHYKRPQPFDDMYMHPFEGAVYYLVLYGPPFVFSMHVSAFAIYMIIMGTFGVLDHSGLPFRIPLVYNSEDHAMHHAKVIVNLGFPFPYWDILHGTFEGEFAGINFHRP 247          
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: A0A485KEW3_9STRA (Aste57867_4084 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KEW3_9STRA)

HSP 1 Score: 163 bits (412), Expect = 1.320e-46
Identity = 79/178 (44.38%), Postives = 113/178 (63.48%), Query Frame = 3
Query:   18 NLSVATLFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTYFGQYCGMEF 551
            NL +A  F   + E  VRG + + FD+   ++     V    V A++  ++ EYYWH L+H   +Y R HK HHHYK P+PFDDMY+HP E   Y+CI + P  VL ++  +F LY+V++G  GV+DHSG SF +PL+Y + DH  HH   NVNY FPF ++DL+H T+ G++ G+ F
Sbjct:   74 NLIMAATFAMLSTELHVRGHSRMCFDD---SSPPSSLVVSQAVLAMLGSNLVEYYWHRLLHIRALYVRVHKVHHHYKRPQPFDDMYMHPFEGAIYFCILYGPPFVLPMNVHAFILYMVVMGLFGVLDHSGLSFHIPLVYKAQDHAVHHAKVNVNYGFPFVYLDLVHGTFHGEFAGVTF 248          
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: W7TYR2_9STRA (C-4 sterol methyl oxidase n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TYR2_9STRA)

HSP 1 Score: 162 bits (410), Expect = 6.740e-46
Identity = 82/175 (46.86%), Postives = 114/175 (65.14%), Query Frame = 3
Query:   18 NLSVATLFMTFTVERIVRG--DTFLTFDEFGITAVDLRKVFLG----LVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 524
            NL +A+LF   T E  VRG      T     +   ++    LG    +V +L +QS  EYYWH LMH GP+Y+RFHK HH YK P PFDDMYI P+E+ GYY I ++PA VL +  +SF++Y+ +LG CG++DHSG   ++P +Y++  H+ HH + N N+ FPFPF+D+LH TY
Sbjct:  122 NLMIASLFALLTAEGTVRGIGSQLKTGHPHVLGTREVIWWLLGESWLVVASLGWQSAWEYYWHRLMHYGPVYRRFHKHHHFYKAPAPFDDMYIAPLEALGYYFILYSPALVLPMHVLSFAVYLTLLGLCGILDHSGIDVRLPRVYDTRFHDIHHSYTNANFGFPFPFLDVLHGTY 296          
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: A0A1V9YQH7_9STRA (Sterol desaturase n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YQH7_9STRA)

HSP 1 Score: 153 bits (387), Expect = 4.230e-43
Identity = 76/169 (44.97%), Postives = 107/169 (63.31%), Query Frame = 3
Query:   18 NLSVATLFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 524
            NL VA++F   T E   RG + ++F     TA+ L +V    + A VF++  EYYWH ++HT  MY R HK HHHYK PRPFDDMY+HP E   YY + + P  VL +   SF +Y+V++GT G++DHSG   ++P +Y++ DH+ HH     NY FPF  +D++H TY
Sbjct:   73 NLLVASVFAMATAECHARGLSRMSFGPAPSTAMLLLQV----LGACVFENTIEYYWHRILHTRAMYTRIHKVHHHYKRPRPFDDMYMHPCEGMAYYWVLYGPPFVLPMHLHSFVVYMVLMGTFGLLDHSGVPVRIPFVYDTRDHDVHHTRVTYNYGFPFQLLDVVHGTY 237          
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: A0A061RXV7_9CHLO (C-4 sterol methyl oxidase n=2 Tax=Tetraselmis sp. GSL018 TaxID=582737 RepID=A0A061RXV7_9CHLO)

HSP 1 Score: 154 bits (388), Expect = 5.910e-43
Identity = 82/172 (47.67%), Postives = 108/172 (62.79%), Query Frame = 3
Query:   15 LNLSVATLFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFA-PAHVL-KISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 524
            +NL  A+ F+ F  E  +RG   +   E   T + L   +L  ++A + QS+  YYWH LMH  P YK  HK HH YK PRPFDD++IHP+E+FGYY + +  PAH L ++    ++LY+ I G  GV+DHSG    VP  Y+  DH+AHHR FNVNYAFPF  MD+ H TY
Sbjct:   86 INLLTASSFLAFVTEAHIRGWNNIKLPE-PDTLIYLE--WLPFLRATLAQSVLVYYWHRLMHWRPCYKALHKLHHFYKAPRPFDDLFIHPVEAFGYYTLLYLFPAHCLGRMPLGDYALYLTIHGVFGVLDHSGIYLSVPPFYHVSDHDAHHRHFNVNYAFPFVLMDIFHGTY 254          
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: A0A067CC58_SAPPC (Fatty acid hydroxylase domain-containing protein n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067CC58_SAPPC)

HSP 1 Score: 152 bits (385), Expect = 7.360e-43
Identity = 77/169 (45.56%), Postives = 108/169 (63.91%), Query Frame = 3
Query:   18 NLSVATLFMTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTY 524
            NL VA++F     E  VRG + +TF   G +   L    L +  A VF++  EYYWH  +HT   Y R HK HH+YK PRPFDDMY+HP+E   YY + + P +V  +   SFS+Y+ ++G  G++DHSG + ++PL+Y++ DHE HHR  N NY FPF ++D+LH TY
Sbjct:   66 NLLVASVFAMMAAEGHVRGLSRMTF---GPSPPPLSMA-LQVGLACVFENTVEYYWHRFLHTRAPYARVHKVHHYYKSPRPFDDMYMHPVEGMAYYWVLYGPPYVFAMHWQSFSVYMALMGLFGLLDHSGIAVRMPLVYDTRDHELHHRRVNANYGFPFQYLDVLHGTY 230          
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: A0A7S3D0N0_9EUKA (Hypothetical protein n=4 Tax=Palpitomonas bilix TaxID=652834 RepID=A0A7S3D0N0_9EUKA)

HSP 1 Score: 151 bits (382), Expect = 4.450e-42
Identity = 75/184 (40.76%), Postives = 112/184 (60.87%), Query Frame = 3
Query:    3 IFVFLNLSVATLFMTFTVERIVRGDTFLTFDEF-GITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTYFGQYCGMEF 551
            IF  +N+ +     T  +E + RG + + F+       + +   FLG    +V   + EYYWH LMH    Y  FHK+HH++K P PFDDMYIHP+E+ GYY I ++   ++ +   +F+ Y++++G  GV+DHSG  F  PL Y S+DH+ HH  + VNY+FPF +MD+LH T+ G Y G+ F
Sbjct:   76 IFATMNVLLQVACATTAIELVCRGYSSMRFEPVENEWKMSIVSFFLG----IVLHMMLEYYWHRLMHLRWFYCTFHKYHHYFKSPEPFDDMYIHPLEALGYYIIMYSTPFLIPMHWTAFAGYMIVVGVAGVMDHSGIKFHFPLFYASEDHDLHHEKYEVNYSFPFMWMDILHGTFRGTYAGITF 255          
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Match: A0A024URC2_9STRA (Fatty acid hydroxylase domain-containing protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024URC2_9STRA)

HSP 1 Score: 147 bits (372), Expect = 2.190e-41
Identity = 74/181 (40.88%), Postives = 106/181 (58.56%), Query Frame = 3
Query:   18 NLSVATLFMTFTVERIVRGDTFLTFD----EFGITAVDLRKVFLGLVKALVFQSICEYYWHLLMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKISAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFPFPFMDLLHQTYFGQYCGME 548
            NL +A  +     E  VRG + + FD     +   AV +    LG        +  EYYWH L+HT  +Y R HK HH+YK PRPFDDMY+HP+E   Y+ I + P  V+ +  VSF +Y++++G  GV+DHSG SF++PL+Y S+DH  HH   + N  FP P+ D+LH T+ G +  + 
Sbjct:   23 NLLLAATYAMAAAELHVRGRSAMVFDAEWPSWKTIAVQVALAILG-------ANCVEYYWHRLLHTRFLYTRVHKVHHYYKRPRPFDDMYMHPVEGAAYFFILYGPPFVMSMHVVSFVIYMMVMGLFGVMDHSGLSFRIPLVYTSNDHATHHAKVHFNLGFPLPYWDILHGTHDGDFAALS 196          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig940.6.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A482SNF4_9ARCH1.170e-5346.15Fatty acid hydroxylase family protein n=1 Tax=arch... [more]
L1JX35_GUITC5.860e-4847.49Sterol desaturase n=2 Tax=Guillardia theta TaxID=5... [more]
A0A6G0WDA8_9STRA1.120e-4743.65Fatty acid hydroxylase domain-containing protein n... [more]
A0A485KEW3_9STRA1.320e-4644.38Aste57867_4084 protein n=1 Tax=Aphanomyces stellat... [more]
W7TYR2_9STRA6.740e-4646.86C-4 sterol methyl oxidase n=1 Tax=Nannochloropsis ... [more]
A0A1V9YQH7_9STRA4.230e-4344.97Sterol desaturase n=1 Tax=Achlya hypogyna TaxID=12... [more]
A0A061RXV7_9CHLO5.910e-4347.67C-4 sterol methyl oxidase n=2 Tax=Tetraselmis sp. ... [more]
A0A067CC58_SAPPC7.360e-4345.56Fatty acid hydroxylase domain-containing protein n... [more]
A0A7S3D0N0_9EUKA4.450e-4240.76Hypothetical protein n=4 Tax=Palpitomonas bilix Ta... [more]
A0A024URC2_9STRA2.190e-4140.88Fatty acid hydroxylase domain-containing protein n... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig940contigH-akashiwo_Contig940:256996..257595 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog55529.EKX53146
PFAMsFA_hydroxylase
Model size600
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons1
Evalue1.84e-49
EggNOG OGsCOG3000@1|root,KOG0873@2759|Eukaryota
DescriptionC-4 methylsterol oxidase activity
Cds size546
COG categoryI
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388052.249902-UTR-H-akashiwo_Contig940:256995..2570361681388052.249902-UTR-H-akashiwo_Contig940:256995..257036Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig940 256996..257036 +
1681388052.2770715-UTR-H-akashiwo_Contig940:257582..2575951681388052.2770715-UTR-H-akashiwo_Contig940:257582..257595Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig940 257583..257595 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388052.2669325-CDS-H-akashiwo_Contig940:257036..2575821681388052.2669325-CDS-H-akashiwo_Contig940:257036..257582Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig940 257037..257582 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig940.6.1prot_H-akashiwo_Contig940.6.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig940 257037..257582 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig940.6.1

>prot_H-akashiwo_Contig940.6.1 ID=prot_H-akashiwo_Contig940.6.1|Name=mRNA_H-akashiwo_Contig940.6.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=182bp
MTFTVERIVRGDTFLTFDEFGITAVDLRKVFLGLVKALVFQSICEYYWHL
LMHTGPMYKRFHKFHHHYKVPRPFDDMYIHPIESFGYYCIFFAPAHVLKI
SAVSFSLYVVILGTCGVIDHSGCSFKVPLLYNSDDHEAHHRFFNVNYAFP
FPFMDLLHQTYFGQYCGMEFCPPRPSVKQKM*
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mRNA from alignment at H-akashiwo_Contig940:256996..257595+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig940.6.1 ID=mRNA_H-akashiwo_Contig940.6.1|Name=mRNA_H-akashiwo_Contig940.6.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=600bp|location=Sequence derived from alignment at H-akashiwo_Contig940:256996..257595+ (Heterosigma akashiwo CCMP452)
GGATTTTCGTTTTTCTTAACCTTTCAGTTGCTACACTGTTCATGACTTTC ACAGTTGAGAGAATAGTACGAGGGGACACCTTCTTGACGTTTGATGAATT TGGAATCACTGCAGTGGACTTGAGAAAAGTTTTTCTGGGTTTGGTCAAAG CTCTCGTTTTTCAGTCTATTTGTGAGTATTATTGGCATTTACTCATGCAC ACAGGACCCATGTACAAGAGGTTCCACAAATTTCATCACCACTACAAAGT TCCCAGGCCCTTTGACGATATGTACATCCACCCTATAGAGTCCTTTGGGT ACTATTGCATTTTTTTTGCGCCAGCCCATGTTCTGAAAATTTCAGCAGTC AGCTTCAGTCTTTATGTGGTAATTTTGGGGACCTGTGGTGTCATTGATCA TTCAGGATGCAGTTTCAAGGTCCCTTTGCTATACAATAGTGATGATCATG AAGCGCATCATCGGTTCTTCAATGTCAACTATGCATTTCCATTTCCTTTT ATGGACCTGCTGCACCAAACCTATTTTGGCCAATACTGTGGTATGGAATT TTGCCCTCCACGACCAAGTGTAAAGCAGAAAATGTAATTAATTTCCCCCC
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Coding sequence (CDS) from alignment at H-akashiwo_Contig940:256996..257595+

>mRNA_H-akashiwo_Contig940.6.1 ID=mRNA_H-akashiwo_Contig940.6.1|Name=mRNA_H-akashiwo_Contig940.6.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=546bp|location=Sequence derived from alignment at H-akashiwo_Contig940:256996..257595+ (Heterosigma akashiwo CCMP452)
ATGACTTTCACAGTTGAGAGAATAGTACGAGGGGACACCTTCTTGACGTT
TGATGAATTTGGAATCACTGCAGTGGACTTGAGAAAAGTTTTTCTGGGTT
TGGTCAAAGCTCTCGTTTTTCAGTCTATTTGTGAGTATTATTGGCATTTA
CTCATGCACACAGGACCCATGTACAAGAGGTTCCACAAATTTCATCACCA
CTACAAAGTTCCCAGGCCCTTTGACGATATGTACATCCACCCTATAGAGT
CCTTTGGGTACTATTGCATTTTTTTTGCGCCAGCCCATGTTCTGAAAATT
TCAGCAGTCAGCTTCAGTCTTTATGTGGTAATTTTGGGGACCTGTGGTGT
CATTGATCATTCAGGATGCAGTTTCAAGGTCCCTTTGCTATACAATAGTG
ATGATCATGAAGCGCATCATCGGTTCTTCAATGTCAACTATGCATTTCCA
TTTCCTTTTATGGACCTGCTGCACCAAACCTATTTTGGCCAATACTGTGG
TATGGAATTTTGCCCTCCACGACCAAGTGTAAAGCAGAAAATGTAA
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