mRNA_H-akashiwo_Contig9.77.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig9.77.1
Unique NamemRNA_H-akashiwo_Contig9.77.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: A0A6V1LYK6_HETAK (Hypothetical protein n=2 Tax=Sar TaxID=2698737 RepID=A0A6V1LYK6_HETAK)

HSP 1 Score: 224 bits (571), Expect = 4.380e-73
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 1
Query:    1 LKAKTIESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAENEA 351
            LKAKTIESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAENEA
Sbjct:    4 LKAKTIESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAENEA 120          
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: G4YYZ2_PHYSP (Ribosomal_S10 domain-containing protein n=7 Tax=Oomycota TaxID=4762 RepID=G4YYZ2_PHYSP)

HSP 1 Score: 178 bits (452), Expect = 5.700e-55
Identity = 88/114 (77.19%), Postives = 106/114 (92.98%), Query Frame = 1
Query:    4 KAKTIESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAEN 345
            K KT+E+EE++IHRIRITL+S NV+NLEKVCA+LKRGAVDK L+VSGPVRLPTK+LR T RK+P GEG+ TWDR +MRIHKRIIDLH+PS+IVKQITSISIEPGV+VEVT+A++
Sbjct:    6 KPKTVEAEETRIHRIRITLSSRNVKNLEKVCADLKRGAVDKNLKVSGPVRLPTKILRLTTRKSPCGEGTNTWDRFEMRIHKRIIDLHAPSDIVKQITSISIEPGVEVEVTIADS 119          
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: H3GAS8_PHYRM (Ribosomal_S10 domain-containing protein n=9 Tax=Eukaryota TaxID=2759 RepID=H3GAS8_PHYRM)

HSP 1 Score: 178 bits (452), Expect = 5.700e-55
Identity = 88/114 (77.19%), Postives = 106/114 (92.98%), Query Frame = 1
Query:    4 KAKTIESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAEN 345
            K KT+E+EE++IHRIRITL+S NV+NLEKVCA+LKRGAVDK L+VSGPVRLPTK+LR T RK+P GEG+ TWDR +MRIHKRIIDLH+PS+IVKQITSISIEPGV+VEVT+A++
Sbjct:    6 KQKTVEAEETRIHRIRITLSSRNVKNLEKVCADLKRGAVDKNLKVSGPVRLPTKILRLTTRKSPCGEGTNTWDRFEMRIHKRIIDLHAPSDIVKQITSISIEPGVEVEVTIADS 119          
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: A0A329RI77_9STRA (Ribosomal_S10 domain-containing protein n=11 Tax=Phytophthora TaxID=4783 RepID=A0A329RI77_9STRA)

HSP 1 Score: 180 bits (456), Expect = 6.110e-55
Identity = 89/114 (78.07%), Postives = 106/114 (92.98%), Query Frame = 1
Query:    4 KAKTIESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAEN 345
            K KT+E+EE++IHRIRITLTS NV+NLEKVCA+LKRGAVDK L+VSGPVRLPTK+LR T RK+P GEG+ TWDR +MRIHKRIIDLH+PS+IVKQITSISIEPGV+VEVT+A++
Sbjct:   52 KPKTVEAEETRIHRIRITLTSRNVKNLEKVCADLKRGAVDKNLKVSGPVRLPTKILRLTTRKSPCGEGTNTWDRFEMRIHKRIIDLHAPSDIVKQITSISIEPGVEVEVTIADS 165          
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: A0A421F2P5_9STRA (Ribosomal_S10 domain-containing protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A421F2P5_9STRA)

HSP 1 Score: 177 bits (449), Expect = 4.380e-54
Identity = 87/114 (76.32%), Postives = 106/114 (92.98%), Query Frame = 1
Query:    4 KAKTIESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAEN 345
            K KT+E+EE++IHRIRITL+S NV+NLEKVCA+LKRGAVDK L+V+GPVRLPTK+LR T RK+P GEG+ TWDR +MRIHKRIIDLH+PS+IVKQITSISIEPGV+VEVT+A++
Sbjct:   37 KPKTVEAEETRIHRIRITLSSRNVKNLEKVCADLKRGAVDKNLKVAGPVRLPTKILRLTTRKSPCGEGTNTWDRFEMRIHKRIIDLHAPSDIVKQITSISIEPGVEVEVTIADS 150          
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: A0A397FH44_9STRA (Ribosomal_S10 domain-containing protein n=8 Tax=Saprolegniaceae TaxID=4764 RepID=A0A397FH44_9STRA)

HSP 1 Score: 176 bits (446), Expect = 8.810e-54
Identity = 88/109 (80.73%), Postives = 101/109 (92.66%), Query Frame = 1
Query:   19 ESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAEN 345
            E EES+IHRIRITLTS NV+NLEKVCA+LK+GAVDK L+VSGPVRLPTK+LR T RK+P GEG+ TWDR +MRIHKRIIDLHSPSEIVKQITSISIEPGV+VEVT+A++
Sbjct:   31 EGEESRIHRIRITLTSRNVKNLEKVCADLKKGAVDKNLKVSGPVRLPTKILRLTTRKSPCGEGTNTWDRFEMRIHKRIIDLHSPSEIVKQITSISIEPGVEVEVTIADS 139          
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: UPI0014259BF5 (40S ribosomal protein S20 n=1 Tax=Anneissia japonica TaxID=1529436 RepID=UPI0014259BF5)

HSP 1 Score: 171 bits (433), Expect = 4.280e-52
Identity = 85/107 (79.44%), Postives = 97/107 (90.65%), Query Frame = 1
Query:   22 SEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAE 342
            +E+  +HRIRITLTS NV+NLEKVCAELKRGA DK L+V GP+R+PTK+LR T RKTP GEGSKTWDR QMRIHKR+IDLHSPSEIVKQITSISIEPGV+VEVT+A+
Sbjct:   12 AEDVTVHRIRITLTSRNVKNLEKVCAELKRGAQDKNLQVKGPIRMPTKILRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIAD 118          
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: A0A1Y1XN71_9FUNG (40S ribosomal protein S20-like protein n=3 Tax=Zoopagomycota TaxID=1913638 RepID=A0A1Y1XN71_9FUNG)

HSP 1 Score: 170 bits (431), Expect = 9.180e-52
Identity = 87/108 (80.56%), Postives = 97/108 (89.81%), Query Frame = 1
Query:   19 ESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAE 342
            E  E+KIH+IRITLTS NV+NLEKVCA+L   A DK+LRV GPVRLPTKVLR T RKTPNGEGSKTWDR +MRIHKR+IDLHSPSEIVKQITSISIEPGV+VEVT+A+
Sbjct:   13 EQVEAKIHKIRITLTSRNVKNLEKVCADLVSRAKDKQLRVKGPVRLPTKVLRITTRKTPNGEGSKTWDRFEMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIAD 120          
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: A0A7S1ZI10_TRICV (Hypothetical protein (Fragment) n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZI10_TRICV)

HSP 1 Score: 170 bits (431), Expect = 9.180e-52
Identity = 90/113 (79.65%), Postives = 100/113 (88.50%), Query Frame = 1
Query:    1 LKAKTIESEESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLA 339
            LKAK  E EE+KIHRIRITLTS NV+NLEKVCA+L RGA DKKL+V GPVRLPTKVLR T RK+P GEG+ TWDR +MRIHKR+IDLHSPSE VKQITSISIEPGV+VEVT+A
Sbjct:   10 LKAKAFE-EEAKIHRIRITLTSRNVRNLEKVCADLIRGAKDKKLKVKGPVRLPTKVLRITTRKSPCGEGTNTWDRFEMRIHKRLIDLHSPSETVKQITSISIEPGVEVEVTIA 121          
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Match: A0A5N5SPA9_9CRUS (40S ribosomal protein S20 n=1 Tax=Armadillidium nasatum TaxID=96803 RepID=A0A5N5SPA9_9CRUS)

HSP 1 Score: 170 bits (430), Expect = 1.220e-51
Identity = 86/106 (81.13%), Postives = 96/106 (90.57%), Query Frame = 1
Query:   25 EESKIHRIRITLTSLNVQNLEKVCAELKRGAVDKKLRVSGPVRLPTKVLRHTMRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEVTLAE 342
            EESKIHRIRITLTS NV++LEKVC EL +GA ++KL+V GPVRLPTK+LR T RKTP GEGSKTWDR QMRIHKR+IDLHSPSE+VKQITSISIEP VDVEVT+AE
Sbjct:   14 EESKIHRIRITLTSSNVKSLEKVCTELVKGAKEEKLKVKGPVRLPTKILRITTRKTPCGEGSKTWDRFQMRIHKRVIDLHSPSEVVKQITSISIEPDVDVEVTIAE 119          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig9.77.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6V1LYK6_HETAK4.380e-73100.00Hypothetical protein n=2 Tax=Sar TaxID=2698737 Rep... [more]
G4YYZ2_PHYSP5.700e-5577.19Ribosomal_S10 domain-containing protein n=7 Tax=Oo... [more]
H3GAS8_PHYRM5.700e-5577.19Ribosomal_S10 domain-containing protein n=9 Tax=Eu... [more]
A0A329RI77_9STRA6.110e-5578.07Ribosomal_S10 domain-containing protein n=11 Tax=P... [more]
A0A421F2P5_9STRA4.380e-5476.32Ribosomal_S10 domain-containing protein n=3 Tax=Ph... [more]
A0A397FH44_9STRA8.810e-5480.73Ribosomal_S10 domain-containing protein n=8 Tax=Sa... [more]
UPI0014259BF54.280e-5279.4440S ribosomal protein S20 n=1 Tax=Anneissia japoni... [more]
A0A1Y1XN71_9FUNG9.180e-5280.5640S ribosomal protein S20-like protein n=3 Tax=Zoo... [more]
A0A7S1ZI10_TRICV9.180e-5279.65Hypothetical protein (Fragment) n=1 Tax=Trieres ch... [more]
A0A5N5SPA9_9CRUS1.220e-5181.1340S ribosomal protein S20 n=1 Tax=Armadillidium na... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig9contigH-akashiwo_Contig9:5653260..5654820 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog79684.XP_005345768.1
Preferred nameRPS20
PFAMsRibosomal_S10
Model size523
Max annot lvl33208|Metazoa
KEGG koko:K02969
KEGG Pathwayko03010,map03010
KEGG ModuleM00177
Hectar predicted targeting categoryno signal peptide or anchor
GOsGO:0000184,GO:0000956,GO:0002181,GO:0003674,GO:0003735,GO:0005198,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005829,GO:0005840,GO:0006139,GO:0006401,GO:0006402,GO:0006412,GO:0006413,GO:0006518,GO:0006605,GO:0006612,GO:0006613,GO:0006614,GO:0006725,GO:0006807,GO:0006810,GO:0006886,GO:0008104,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009058,GO:0009059,GO:0009892,GO:0009987,GO:0010467,GO:0010468,GO:0010605,GO:0010629,GO:0015031,GO:0015833,GO:0015935,GO:0016070,GO:0016071,GO:0019222,GO:0019439,GO:0019538,GO:0022626,GO:0022627,GO:0031974,GO:0031981,GO:0032991,GO:0033036,GO:0033365,GO:0034613,GO:0034641,GO:0034645,GO:0034655,GO:0042886,GO:0043043,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044260,GO:0044265,GO:0044267,GO:0044270,GO:0044271,GO:0044391,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044445,GO:0044446,GO:0044464,GO:0045047,GO:0045184,GO:0046483,GO:0046700,GO:0046907,GO:0048519,GO:0050789,GO:0051179,GO:0051234,GO:0051641,GO:0051649,GO:0060255,GO:0065007,GO:0070013,GO:0070727,GO:0070972,GO:0071702,GO:0071704,GO:0071705,GO:0072594,GO:0072599,GO:0072657,GO:0090150,GO:0090304,GO:1901360,GO:1901361,GO:1901564,GO:1901566,GO:1901575,GO:1901576,GO:1990904
Exons3
Evalue1.46e-26
EggNOG OGsCOG0051@1|root,KOG0900@2759|Eukaryota,3A3H9@33154|Opisthokonta,3BQ9M@33208|Metazoa,3D79I@33213|Bilateria,48EM2@7711|Chordata,49BPB@7742|Vertebrata,3JGT6@40674|Mammalia,35PPF@314146|Euarchontoglires,4Q5QQ@9989|Rodentia
Ec32 ortholog descriptionRibosomal protein S10, eukaryotic/archaeal
Ec32 orthologEc-16_004860.1
Descriptioncytoplasmic translation
Cds size174
COG categoryJ
BRITEbr01610,ko00000,ko00001,ko00002,ko03011
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388032.1196313-UTR-H-akashiwo_Contig9:5653259..56534281681388032.1196313-UTR-H-akashiwo_Contig9:5653259..5653428Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig9 5653260..5653428 -
1681388032.1557627-UTR-H-akashiwo_Contig9:5654210..56542991681388032.1557627-UTR-H-akashiwo_Contig9:5654210..5654299Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig9 5654211..5654299 -
1681388032.1687162-UTR-H-akashiwo_Contig9:5654729..56548201681388032.1687162-UTR-H-akashiwo_Contig9:5654729..5654820Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig9 5654730..5654820 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388032.1332831-CDS-H-akashiwo_Contig9:5653428..56535461681388032.1332831-CDS-H-akashiwo_Contig9:5653428..5653546Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig9 5653429..5653546 -
1681388032.1451998-CDS-H-akashiwo_Contig9:5654154..56542101681388032.1451998-CDS-H-akashiwo_Contig9:5654154..5654210Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig9 5654155..5654210 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig9.77.1prot_H-akashiwo_Contig9.77.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig9 5653429..5654210 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig9.77.1

>prot_H-akashiwo_Contig9.77.1 ID=prot_H-akashiwo_Contig9.77.1|Name=mRNA_H-akashiwo_Contig9.77.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=58bp
MRKTPNGEGSKTWDRLQMRIHKRIIDLHSPSEIVKQITSISIEPGVDVEV
TLAENEA*
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mRNA from alignment at H-akashiwo_Contig9:5653260..5654820-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig9.77.1 ID=mRNA_H-akashiwo_Contig9.77.1|Name=mRNA_H-akashiwo_Contig9.77.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=1561bp|location=Sequence derived from alignment at H-akashiwo_Contig9:5653260..5654820- (Heterosigma akashiwo CCMP452)
CTTAAAGCCAAAACGATTGAATCCGAGGAATCCAAGATCCACCGGATCCG GATCACTCTGACTTCCCTGAATGTTCAGAACCTCGAGAAAGGTGCAGTAA ATTCCTATAGATAAAAATATTTCAGCGTTTATATATGGCATTTTTAAATC CAGAAAAATGCTTGCAACCAAATGATGCTCTTCGCAACTTTCGTTCTACC GCAAGAGCTCTACTCACTGTGGAGATCATAGAAGCTGATGGTTGCCGTGG ATGGCCCCACCGATAACTTGATAACGCCACACTGATATTGAGCCATTTAA CACTCCATTCACCCCACATACTAAATCGGCTTCCTCATCCGCAGAGCCCA TTATTACTTTCACCATCATCACCTTTTTTCATGCCCTAGAAGAAATGGTT CAGGTGAAGAGGCTGCTGCACGTCTGTGCCCACCTTTGTGACCCGCCTTA TCCCTCATGGCCCCGTCCCGCCTCCGTTAACCCCCCTCCCCCCCCCCCCC CCCCCCTACCCCCGCCCCCAGTGTGCGCCGAGCTGAAGCGCGGCGCTGTG GACAAGAAGCTGCGCGTGTCCGGCCCCGTGCGCCTGCCCACCAAGGTGCT CCGGCACACCATGCGCAAGACCCCCAACGGCGAGGGGTCCAAGACCTGGG ATCGCCTCCAGATGAGGTGAGAGAGTGGCGCGATGCTCGACCACATCCAC AACGGTCGTGGACCTGGCGGGGGTGGCAGTTGGACTTCCCACCAGACCGG GCCAGACGCCAGCCGTGCTTTGGAATGAAATCACAATGCAAGATACTAAC GAGATTTCTGGAACTCTGCTACAAAGGCATGACGTGTGTGTCAGTCAGCG TGTAGTGGGGGGTGCCAAAATCTGATCTCGCCAGCATCATACCAGTGCAA TCTCAAAAGTAACACTGCCCGTAAAGGACATTGAGATGGAACAGCAAGTG AAGAGCATGGCGTGCACAGCGTAAAAATAAAAAAGTAAAAGAATCAGGAC TATACTTCATACAAAACAAAACATAACTTTTCATGCCCTGATCAATAATA TGTAGGTGAATCAAATATCATGGTCATTGGATACTCCTCCATACCATCGT GTTTATAATCTCATCTTCCTTTCACTTCCTCCTCTTTCATAACCCCCCAA ATGCAAGCATGGAATGGGCTGGATGAATTTACCCATATACATTGTGGCTC CTTCCCCCCCCCCCCCCCGTCGATGATTCACCGCATGCCGCCTGTCCTCC CCTGCCCCGGCCCCGACCGCGCAGGATCCACAAGCGCATCATCGACCTGC ACTCCCCCTCGGAGATCGTGAAGCAGATCACCTCCATCTCCATCGAGCCC GGGGTGGACGTCGAGGTCACCCTGGCGGAGAATGAGGCCTAAGGGGCGGC GGTGCATCGGCATGACCAGTTCCAGCCTTCTTGCTGAATAAAGAATGCAG GCTGAACAAAAAAACTCCATGTACCTATTGTTTGTCTTACATGTGATCAA GCTCCAAGGTAGAAAAGTCGAATGATTTATAAAAAATTATTATGTTTGGT TGTTGTTGTTA
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Coding sequence (CDS) from alignment at H-akashiwo_Contig9:5653260..5654820-

>mRNA_H-akashiwo_Contig9.77.1 ID=mRNA_H-akashiwo_Contig9.77.1|Name=mRNA_H-akashiwo_Contig9.77.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=174bp|location=Sequence derived from alignment at H-akashiwo_Contig9:5653260..5654820- (Heterosigma akashiwo CCMP452)
ATGCGCAAGACCCCCAACGGCGAGGGGTCCAAGACCTGGGATCGCCTCCA
GATGAGGATCCACAAGCGCATCATCGACCTGCACTCCCCCTCGGAGATCG
TGAAGCAGATCACCTCCATCTCCATCGAGCCCGGGGTGGACGTCGAGGTC
ACCCTGGCGGAGAATGAGGCCTAA
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