mRNA_H-akashiwo_Contig847.3.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig847.3.1
Unique NamemRNA_H-akashiwo_Contig847.3.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: A0A7S3UU75_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UU75_HETAK)

HSP 1 Score: 508 bits (1309), Expect = 9.090e-177
Identity = 259/268 (96.64%), Postives = 261/268 (97.39%), Query Frame = 1
Query:  274 RELGFVTEQDKANYAELLARAREQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKANTKLTTSSKGLST-NWRRISIIGTKG 1074
            RELGFVTEQDKANYAELLARAREQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKANTKLTTSSKGLST N  +   +G KG
Sbjct:    1 RELGFVTEQDKANYAELLARAREQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKANTKLTTSSKGLSTVNEEKSEAVGGKG 268          
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: D7FP09_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FP09_ECTSI)

HSP 1 Score: 411 bits (1056), Expect = 5.100e-133
Identity = 243/588 (41.33%), Postives = 333/588 (56.63%), Query Frame = 1
Query:    1 TTWTKTADHHDYLQSDLLPDC-EDVTAYAELLAVLDSPVGQRHLGVYAKKIFCQELLLFWTDCQVYRSIPTCDYRRAKGGNIHNKYIKSGAVRELGFVTEQDKANYAELLARAREQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVD-DESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKANTKLTTSSKGLSTNWRRISIIGTKGYMAPEVVKLMSPADGPRKGTPRP---WTTG-----------RWAPPPTSC*PGGGRSSNPKSLFPQQPPPAPQRAREPSEQKGGVPQPR--QPEAKSFIKECLDVNEETRMG-GRGGRQRLENHPWFASVDWAALLARTVRVPHVPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSVRAPNR---EQQKYFRSWNYVSPHTLKVEMGIANESRALTTNFKAQQMMG 1698
            T W K   H D+ +S +LPD  ED+T Y ELLA+L SPVGQ  LG ++ +I  Q +L  W+D Q YR+IPT DYR  K   I  KY+++ A   L F+ E         L +ARE   LLG +L+  + K CF  IF+  F   K +    Y A++EE  + +N +GV DF YME LG GAFG+VVHV KKSTG HYAMKIQ K +L+ +F   L++L+NEK VF ACNHPF+L M YAFQTEL+ ++VL LVT GNLQDAID S   RL E +ARFY AE+ +AL HLHDMGLMYRDLKPRNV+L  DGH+ LADMGGVGD  G + ++ ++K  T  T   +G   N++R SI+GTKGYMAPE+ KL++ ++  R+G       W+ G            + PPP +      R  +  ++         +R  E  + +  V  PR     A SF+   L  +   R+G G  G++ +++H + A + W  L A+ V  P++P                              +      +   + QK+F +W Y+SP TL++EMG+A     +  NFK  Q+ G
Sbjct:   27 TVWRKEGYHDDFTKSPILPDDREDITEYVELLAMLHSPVGQNFLGRFSMEIGTQHVLFAWSDIQEYRAIPTVDYRFTKAKQIFGKYLRASATA-LDFLPEGLGEEIYNCLDQAREDKNLLGPDLFLQVQKVCFRHIFEFTFQELKNNYARRYEAYQEEGASRFNRIGVNDFWYMEKLGEGAFGKVVHVVKKSTGRHYAMKIQHKTELLRSFARSLSRLDNEKMVFQACNHPFVLQMDYAFQTELHAIIVLNLVTTGNLQDAIDSSPKKRLDEPRARFYAAEIVIALIHLHDMGLMYRDLKPRNVMLGMDGHIQLADMGGVGDCIGDMSARKIRKHGT--TVKRRGTLMNFKRRSIMGTKGYMAPEMAKLLNQSNDQRQGYDEAVDWWSLGVTVYKMLTGARPFDPPPWA------RKQSVVAML--------RRQTEFEKLQAEVEYPRYMSEAAVSFVSALLRHDVHERLGSGPNGKEGIKSHAFMAGIVWERLAAKNVEPPYIPTQGPVCDEPLYDSFEEMMEGMHEADARYNKKKRNLNEKVSPDDQKWFATWRYISPQTLRIEMGLAQAMDDIEANFKVMQLTG 597          
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: A0A7S1U415_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1U415_9STRA)

HSP 1 Score: 362 bits (930), Expect = 1.910e-114
Identity = 226/552 (40.94%), Postives = 313/552 (56.70%), Query Frame = 1
Query:   79 YAELLAVLDSPVGQRHLGVYAKKIFCQELLLFWTDCQVYRSIPTCDYRRAKGGNIHNKYIKSGAVRELGFVTEQDKANYAELLARAREQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKANTKLTTSSKGLSTNWRRISIIGTKGYMAPEVVKLMSPADGPRKGTPRP---WTTGRWAPPPTSC*PGGGRSSNPKSLFPQQPPPAPQRAREPSEQKGGVPQPRQPEAKSFIKEC--------LDVNEETRMGGRG-GRQRLENHPWFASVDWAALLARTVRVPHVPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSVRAPNREQQKYFRSWNYVSPHTLKVEMGIANESRALTTNFKAQQMMG 1698
            + E   +LD P+GQR++G +AK  F  E    W D Q +++IPT DYRR+K  +I++KY+K GAV ELG V  Q+K  +  ++ RAR    ++   ++DD+ +  F++I+   FI FK  D+ Y  +R+E   TYN V V+DFEYME LG G FGRVVH RK STG HYAMKIQ+K  L+  F DD T+L+ EK+VFAAC+HPFI+ + YAFQT  + +L L LVTAG+LQ+ +++    RL E++   YTAEV LAL HLH++GLMYRDLKP NVLL  DGHV LADMGGV DF G+V     +KA+        G+   +RR SI+GT+GYMAPEV+ LMS A   RKG  +    W+ G        C   G R  +  S        + Q+ ++ +E    + +   P   +FI +         + ++ E R+G    G   L  H +F  +D+  L  + +   + P                                   P+  QQ++F  W++VSPHTLKVEMGIA E     TN K QQ++G
Sbjct:   61 HIECRDILDDPIGQRYVGQFAKSNFTHEHFFCWIDMQEFKAIPTKDYRRSKAMHIYDKYLKVGAVLELGSVAPQEKERFKGIVERARGDKSVVTPNMFDDIWQMVFVDIYHNTFIPFKRSDK-YEEYRKEIRETYNKVSVDDFEYMEKLGEGGFGRVVHCRKISTGKHYAMKIQLKTALLDTFWDDPTRLDAEKSVFAACHHPFIVSLDYAFQTPSHAILALGLVTAGDLQEIMEKEPSKRLDEDRVVLYTAEVVLALQHLHELGLMYRDLKPCNVLLGADGHVQLADMGGVADFKGNVLDHRNEKADDD------GV---YRRRSIMGTRGYMAPEVLDLMS-AVSARKGYTQAVDFWSLGVTVYK-LLC---GTRPFDMSSYDVLLEYSSEQKQQKQAEYDNLLHRIYFP---TFISDAGCELIIGLMALHPEDRLGSNAEGVDGLTQHAFFNGMDFIKLGQKHLDAAYKPDVQLPSEKPMYPSYSTMMEAITGPDDRSNFDWESVPSDRQQRHFDEWDFVSPHTLKVEMGIAGEMAVHDTNLKVQQILG 594          
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: A0A835ZAC1_9STRA (Kinase-like domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZAC1_9STRA)

HSP 1 Score: 357 bits (916), Expect = 1.000e-112
Identity = 219/563 (38.90%), Postives = 303/563 (53.82%), Query Frame = 1
Query:   79 YAELLAVLDSPVGQRHLGVYAKKIFCQELLLFWTDCQVYRSIPTCDYRRAKGGNIHNKYIKSGAVRELGFVTEQDKANYAELLARAREQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKAN------------TKLTTSSKGLSTNWRRISIIGTKGYMAPEVVKLMSPADGPRKGTPRP---WTTG-RWAPPPTSC*PGGGRSSNPKSLFPQQPPPAPQRAREPSEQKGGVPQPRQPEAKSFIKECLDVNEETRMGGRGGR-QRLENHPWFASVDWAALLARTVRVPHVPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSV------RAPNREQQKYFRSWNYVSPHTLKVEMGIANESRALTTNFKAQQMMG 1698
            + EL  +L+ PVGQ+++G +AKK    E    WT+ Q YRSIPT DY+R    +I+ KYIK GA+ +LG V   D+     +L +++    +   +LYD + +  F+E++   F RFKV DE YS  R+    TYN V + DF+Y+  +G G FGRVVHVRKKST  H AMKIQ+K  L+  F DD  +++NE+ V AA  HPFI+ M+YAFQTE   ++VL LVT G+LQ+AI+++   RL E + +FY AE+ LAL HLH++GLM+RDLKP NVLL QDGHV L DMGGV +FA       +   +             K  T ++ LS + RR SI+GT GYMAPE+V L+S     RKG  +    W+ G       T   P   +    + L   + PP                     EAK  +   LD NE  R+G        L+ HP+F  +DW  L  R +  P+VP                                       + P+   Q++F+SW+++S  TLK+EMGIANE  +  TNFK +Q+MG
Sbjct:   21 HMELRTILEDPVGQKYIGAFAKKTLTHESFFAWTEIQEYRSIPTADYQRGMAMHIYQKYIKEGAILQLGCVKPADRQCIKAVLDQSKYDASVTNNQLYDKVQQAVFIEMYHNTFQRFKVSDE-YSEMRKMLRETYNRVCLSDFDYIAQIGEGGFGRVVHVRKKSTMAHLAMKIQLKTALLETFSDDPNRIDNERRVLAATYHPFIVGMNYAFQTETLAIMVLDLVTCGDLQEAINQAPNKRLNEVRVQFYVAEIILALSHLHELGLMFRDLKPCNVLLGQDGHVKLTDMGGVAEFAKGTCLDPMDSCSGSSPYMMEIGGQRKGNTEARKLSQSHRRRSIMGTHGYMAPEMVILLSQPRAVRKGYTKAVDFWSLGVTMYKLLTGVRPFENKRFLNEILTEVRFPPY-----------------MSAEAKDLVIRLLDGNEHRRLGASPELLAELKAHPFFNGIDWVRLSVRHMIPPYVPDAKPLEQTPQYASFDALMEAYRTQDEADRSGGTPMCDWTQKPSPADQRFFQSWDFISMQTLKIEMGIANEMVSHDTNFKVRQLMG 565          
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: F0WFN3_9STRA (Protein kinase putative n=2 Tax=Albugo TaxID=65356 RepID=F0WFN3_9STRA)

HSP 1 Score: 338 bits (867), Expect = 3.420e-105
Identity = 205/559 (36.67%), Postives = 297/559 (53.13%), Query Frame = 1
Query:   85 ELLAVLDSPVGQRHLGVYAKKIFCQELLLFWTDCQVYRSIPTCDYRRAKGGNIHNKYIKSGAVRELGFVTEQDKANYAELLARAREQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKAN---TKLTTSSKGLSTNWRRISIIGTKGYMAPEVV--KLMSPADGPRKGTPRPWTTGRWAPPPT------SC*PGGGRSSN-------PKSLFPQQPPPAPQRAREPSEQKGGVPQPRQPEAKSFIKECLDVNEETRMGGRG--GRQRLENHPWFASVDWAALLARTVRVPHVPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRS-VRAPNREQQKYFRSWNYVSPHTLKVEMGIANESRALTTNFKAQQMMG 1698
            ELLAV+  PVGQ+HLG YAKKI   E    W +   +R  P+  YR++    IH KYIKSG+   LG +     A+Y   +        ++  +L+D L+K    +++Q  F+RFK   E Y  +R+E   TYN V V+DFEY+ELLG+G FGRVVH RKKSTG HYAMK+Q+K  L+  ++D L ++ +EK +  AC++PF+LDMHY+FQT  + ++V  LV  G+L  A+  S    L E++ R Y AE+ LAL H+H++GL+YRDLKP NVLL +DGH++LADMG       + F   L K N   T++T ++  L+   RR + +GTKGYMAPE++  KL+  +  P       +    W+   T         P G  SS         + +F   P    Q   +   Q    P       + F+++ L+VN + R+G     G      HP+F S+DW  L+ + +   + PA                                 + P  + QK+F SW+Y+S HTL+ E+GIANE  A    +K  Q+ G
Sbjct:   56 ELLAVVSDPVGQKHLGAYAKKILTSETFFCWVEINDFRDAPSLGYRKSLAKLIHKKYIKSGSPMALGSLPSDVVAHYENHIKAMETNESIVTPDLFDLLMKYVLSDMYQSTFVRFKASAE-YDLYRKEVKETYNRVTVDDFEYLELLGSGGFGRVVHARKKSTGKHYAMKVQLKAGLLDEYQDQLAQITSEKDILQACHNPFVLDMHYSFQTPAHAIIVSELVRGGDLGSALASSRESHLSEDRVRLYAAEIGLALNHMHELGLIYRDLKPVNVLLGEDGHIVLADMG-----LAAGFDTCLLKVNEDGTRVTPATPKLT---RRKTTVGTKGYMAPEIIAGKLVKRSQRPGYT----FAIDYWSLGVTIYELLTGYQPFGVHSSQYVLQQVAQEDMFRMSPRTQHQLELQKMAQGTNYPSHLSRVVQEFLQQLLEVNPDQRLGCSPDHGFAEFMGHPFFDSIDWEKLMVKHIDPTYRPALPPLTDKKIYNSYQQMMGQFDARDKDYIRHDWYKTPPSDMQKHFDSWDYISSHTLRAELGIANELDATNIPYKVLQLTG 601          
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: A0A8K1C8Y3_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1C8Y3_PYTOL)

HSP 1 Score: 323 bits (827), Expect = 2.630e-99
Identity = 203/557 (36.45%), Postives = 290/557 (52.06%), Query Frame = 1
Query:   85 ELLAVLDSPVGQRHLGVYAKKIFCQELLLFWTDCQVYRSIPTCDYRRAKGGNIHNKYIKSGAVRELGFVTEQDKANYAELLARAREQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKK---ANTKLTTSSKGLSTNWRRISIIGTKGYMAPEVVKLMSPADGPRKGTPRP---WTTG------RWAPPPTSC*PGGG--RSSNPKSLFPQQPPPAPQRAREPSEQKGGV--PQPRQPEAKSFIKECLDVNEETRMG--GRGGRQRLENHPWFASVDWAALLARTVRVPHVPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSVRA-PNREQQKYFRSWNYVSPHTLKVEMGIANESRALTTNFKAQQMMG 1698
            ELLA+L+ PVGQ+H+G++AKKI   E    W + + +R  P+  YR++    I+ KYIK+GA   LG +T    A Y + +        L+  + +D L+     ++ Q  ++RFK   E Y  +++E   TYN V VEDFEY+ELLG+G FGRVVH RKKSTG HYAMKIQ+K  L+    D L+++ +EK +   C++PF+LDMHY+FQT  + ++V  LV  G+L +A+  S  G + E++ R Y AE+ LAL H+HD+GL+YRDLKP NVLL +DGHV+LADMG     A       LKK    NTK    +   +   RR + +GT+GYMAPE++         R G       W+ G           P S   GG   +    + +F   P    Q   E  +   GV  P      A+ F++  L+VN E R+G     G Q   +H +F  VDW  L+ + +     P                                  A P+ E QK+F SW+Y+S +TL+ E+GIA E       +K  Q+ G
Sbjct:   61 ELLAILNDPVGQKHIGIFAKKIMTAETFFCWVEIKEFRDAPSIGYRKSLAKLIYKKYIKNGAPMALGSITADIIALYDKKITDMERDATLMTPDFFDRLMTLITTDMVQNTYLRFKTSSE-YDIYKKEMKDTYNKVTVEDFEYLELLGSGGFGRVVHARKKSTGKHYAMKIQLKTGLLDEHHDQLSQITSEKDILQICHNPFVLDMHYSFQTSAHAIIVTELVRGGDLGEALKSSREGYITEDRVRLYAAEIGLALNHMHDLGLIYRDLKPGNVLLGEDGHVVLADMG----LAAGYDPGNLKKDDNGNTKYVPQNAKFT---RRKTTVGTRGYMAPEIISGKLVKRDQRPGYTHAVDYWSLGVTIFELLCGFQPFSVYSGGNFFQEVANEEIFRLSP--RTQHKIELQKMAQGVTFPPHLSKNAQDFLRRLLEVNAEIRLGCSPEKGFQEFMDHAFFEVVDWEKLMMKHIDPKFKPTLPPLTDKKIYNSYDHMMSHFDMRDKDFIRHDWYADPDPEMQKHFDSWDYISQYTLRAELGIAKELDETNIPYKVLQLTG 607          
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: K3X895_GLOUD (Uncharacterized protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X895_GLOUD)

HSP 1 Score: 322 bits (825), Expect = 6.420e-99
Identity = 206/567 (36.33%), Postives = 293/567 (51.68%), Query Frame = 1
Query:   64 EDVTAYAELLAVLDSPVGQRHLGVYAKKIFCQELLLFWTDCQVYRSIPTCDYRRAKGGNIHNKYIKSGAVRELGFVTEQDKANYAELLARAREQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKA-----NTKLTTSSKGLSTNWRRISIIGTKGYMAPEVVKLMSPADGPRKGTPRP---WTTG------RWAPPPTSC*PGGG--RSSNPKSLFPQQPPPAPQRAREPSEQKGGVPQPRQPE--AKSFIKECLDVNEETRMG---GRGGRQRLENHPWFASVDWAALLARTVRVPHVPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSVRA-PNREQQKYFRSWNYVSPHTLKVEMGIANESRALTTNFKAQQMMG 1698
            E  T   ELL +L  PVGQ+H+G +AKKI   E    W + + ++  P+  YR++    I+ KYIK+G+   LG +T +  A Y + +      P L+  E +D L+     ++ Q  F+RFK   E Y A++++   TYN V VEDFEY+ELLG+G FGRVVH RKKSTG HYA+KIQ+K  L+    D L ++ +EK +   C++PFILDM Y+FQT  + ++V  LV  G+L +A+  S  G + E++ R Y AE+ LAL H+H++GL+YRDLKP NVLL +DGHV+LADMG    F  ++  K    +     N KLT          RR + +GT+GYMAPE++         R G       W+ G           P S   GG   +    + +F   P    Q   E  +   GV  P       + F+K  LDVN E R+G    +G  + +E HP+F S+DW  L+ + +     PA                                 A P  EQQK+F SW+Y+S  TL+ E+GIA E       +K  Q+ G
Sbjct:   56 EPSTNEEELLNMLHDPVGQKHIGAFAKKIMTAETFFCWVEVKEFKDAPSIGYRKSLAKLIYKKYIKNGSTMALGSITSELIATYDKKITDMETNPSLMTPEFFDPLMTNIVNDMCQNTFLRFKTTPE-YDAYKKDVKDTYNKVTVEDFEYLELLGSGGFGRVVHARKKSTGKHYAIKIQLKAGLLDEHHDQLGQITSEKDILQVCHNPFILDMQYSFQTPAHAIIVTELVRGGDLGEALKASREGYIIEDRVRLYAAEIGLALNHMHELGLIYRDLKPGNVLLGEDGHVILADMGLAAGFDPTLLKKDENSSKYIPVNPKLT----------RRKTTVGTRGYMAPEIISGKLVKRDQRPGYTFAVDYWSLGVTIFELMCGYQPFSVYSGGNFFQEVANEEIFRLSP--RTQHKIELQKMAQGVTFPAHISKCGQDFLKRLLDVNVEQRLGCNCEKGFAEFME-HPFFESIDWEKLMMKHIDPKFKPALPLLTDKKIYNSYEHMMSHFDMRDKDFIRHDWYADPPPEQQKHFESWDYISQSTLRAELGIAKELDETNIPYKVLQLTG 608          
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: D8LEF7_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LEF7_ECTSI)

HSP 1 Score: 320 bits (819), Expect = 9.620e-99
Identity = 206/560 (36.79%), Postives = 288/560 (51.43%), Query Frame = 1
Query:   85 ELLAVLDSPVGQRHLGVYAKKIFCQELLLFWTDCQVYRSIPTCDYRRAKGGNIHNKYIKSGAVRELGFVTEQDKANYAELLARA----REQPQLLGTELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKANTKLTT----------SSKG---LSTNWRRISIIGTKGYMAPEVVKLMSPADGPRKGTPRP---WTTGRWAPPPTSC*PGGGRSSNPKSLFPQQPPPAPQRAREPSEQKGGVPQPRQ--PEAKSFIKECLDVNEETRMGGRGGRQ-RLENHPWFASVDWAALLARTVRVPHVPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSVR-----APNREQQKYFRSWNYVSPHTLKVEMGIANESRALTTNFK 1680
            E+ A+L+ P+GQ+++G +AKK+  QE    W D Q +RSIPT DYRR K  +I+ KYI+ GAV ++G +  +++A    ++             + T+++D   K  F E+F   F RF    E Y+    +    YN V  +DF Y+E +G G FGRVVHV+KK+T +HYAMKIQ+K  L+  F DD T++++E+ V +  +HPFI+ M YAFQT    ++ L LVT G+LQ+AID +  GR+  ++ +FY+AE+ LAL HLHD+GLMYRDLKP NVLL  DGHV LADMGGV +FA     +T K  N    T          S  G   L+   RR SI+GT+GYMAPE+V L       R G       W+ G                    ++F       P   R   E    +  P     EA+S I   L   E  R+GG       L++H +F  +DW  L  R V  P++P                                       AP  E Q +F SW++VS HTLK+E+GI++E      +FK
Sbjct:   20 EMRAILEDPIGQKYIGQFAKKVMTQESFYAWVDIQEFRSIPTGDYRRGKSMHIYQKYIREGAVLQVGSIPAEERAKIKHIIDAGVVDKAGNKSGISTDMFDFFRKAVFKEMFYNTFQRFSASPE-YAQMHADIKNAYNKVCPDDFLYIERIGEGGFGRVVHVKKKTTQSHYAMKIQLKTALLDTFNDDPTRIDHERRVLSVTHHPFIVGMDYAFQTPELGIMCLELVTGGDLQEAIDTAPEGRIEAKRVQFYSAEIILALVHLHDLGLMYRDLKPCNVLLMGDGHVKLADMGGVAEFADGTCLETNKDKNPFTGTEIGSLPQSKSSEDGPPTLNNKHRRRSIMGTQGYMAPEMVILPKQPRSVRVGYSNAVDYWSLG-------------------VTVFKLLTGSRPFDRRRFQELLDTISWPEYVGSEARSVISGLLACKETERLGGTPENLLALKSHEYFEGIDWLRLSQRHVLPPYLPKVPPLREEPLYQTFDHLMRDFYAQQEMESATKDTYDWFVAPTDEDQAFFNSWDFVSMHTLKIELGISSEMAVHDQSFK 559          
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: H3GJZ7_PHYRM (Uncharacterized protein n=20 Tax=Peronosporaceae TaxID=4777 RepID=H3GJZ7_PHYRM)

HSP 1 Score: 312 bits (799), Expect = 3.130e-95
Identity = 199/560 (35.54%), Postives = 289/560 (51.61%), Query Frame = 1
Query:   64 EDVTAYAELLAVLDSPVGQRHLGVYAKKIFCQELLLFWTDCQVYRSIPTCDYRRAKGGNIHNKYIKSGAVRELGFVTEQDKANYAELLARAREQPQLLGT-ELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKANTKLTTSSKGLSTNWRRISIIGTKGYMAPEVVKLMSPADGPRKGTPRPWTTGRW------APPPTSC*PGGG--RSSNPKSLFPQQPPPAPQRAREPSEQKGGVPQPRQ--PEAKSFIKECLDVNEETRMG---GRGGRQRLENHPWFASVDWAALLARTVRVPHVPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSVRA-PNREQQKYFRSWNYVSPHTLKVEMGIANESRALTTNFKAQQMMG 1698
            E  T   ELL +L  PVGQ+H+G +AKKI   E    W + + +R  P+  YR++    I+ KYIK+G+   LG +T      Y + +A          T + +D L+     ++ Q  F+RFK   E Y  ++++   TYN V +EDFEY+ELLG+G FGRVVH RKKSTG HYAMKIQ+K  L+    D L+++ +EK +   C++PF+LDMHY+FQT  + V+V  LV  G+L DA+  S  G L E++ R Y AE+ LAL H+H++GL+YRDLKP NVLL +DGHV+LADMG    F      K     + + T     L+   RR + +GT+GYMAPE++ +              W+ G           P S    G   +    + +F   P    Q   E  +   GV  P      A+ F+K  L+V+ E R+G    +G  + +E HP+F +VDW  L+ + +     PA                                 A P+ E QK+F +W+Y+S +TL+ E+GIA E       +K  Q+ G
Sbjct:   58 EPSTNEQELLHMLHDPVGQKHIGAFAKKILTSETFFCWVEIKEFREAPSIGYRKSMAKLIYRKYIKNGSTMALGSITSDLIVLYDKKIAELEANHGSSATVDFFDPLMTNIVADMCQNTFMRFKASSE-YETYKKDLKDTYNKVTIEDFEYLELLGSGGFGRVVHARKKSTGKHYAMKIQLKTGLLDEHHDQLSQITSEKDILQICHNPFVLDMHYSFQTPAHAVIVTELVRGGDLGDALKSSRDGYLHEDRVRLYAAEIGLALNHMHELGLVYRDLKPGNVLLGEDGHVILADMGLAAGFDPLKLKKEESSGDFRYTPVLPKLT---RRKTTVGTRGYMAPEIISVDF------------WSLGVTIFELVCGFQPFSMHSSGNFFQEVANEEIFRLSP--RTQHKIELQKMAQGVTFPLHLTRNAQDFLKRLLEVSPEHRLGCNVEKGFTEFME-HPFFETVDWEKLMMKHIDPKFKPALPLLSDKKIYNSYEHMMSHFDMRDKDFIRHDWYADPDPEMQKHFDTWDYISQYTLRAELGIAKELDETNIPYKVLQLTG 598          
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Match: A0A0P1B3D1_PLAHL (Agc protein kinase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1B3D1_PLAHL)

HSP 1 Score: 309 bits (791), Expect = 9.500e-94
Identity = 200/566 (35.34%), Postives = 291/566 (51.41%), Query Frame = 1
Query:   85 ELLAVLDSPVGQRHLGVYAKKIFCQELLLFWTDCQVYRSIPTCDYRRAKGGNIHNKYIKSGAVRELGFVTEQDKANYAELLARAREQPQLLGT-ELYDDLLKRCFLEIFQQIFIRFKVDDESYSAFREESDATYNVVGVEDFEYMELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKTVFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEEQARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDFAGSVFSKTLKKANTKLTTSSKGLSTNWRRISIIGTKGYMAPEVV--KLMSPADGPRKGTP-------------------RPWT---TGRWAPPPTSC*PGGGRSSNPKSLFPQQPPPAPQRAREPSEQKGGVPQPRQPEAKSFIKECLDVNEETRMGGRGGRQRLE--NHPWFASVDWAALLARTVRVPHVPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSVRA-PNREQQKYFRSWNYVSPHTLKVEMGIANESRALTTNFKAQQMMG 1698
            ELL +L  PVGQ+H+G +AKKI   E    W + + +R  P+  YR++    I+ KYIK+G+   LG +T     +Y + +A          T + +D L+     ++ Q  FIRFK   E Y AF+++   TYN V +EDFEY+E+LG+G FGRVVH RKKSTG HYAMKIQ+K  L+    D L+++ +EK +   C++PF+LDMHY+FQT  + ++V  LV  G+L DA+     G + E++ R Y AE+ LAL H+H++GL+YRDLKP NVLL +DGHV+LADMG    F      K  +      T     L+   RR + +GT+GYMAPE+V  KLM   +  R+G                     RP+T   TG +     +    G    +P+S    +         E   Q    P    P A+ F+K  L+V  E R+G    +  +E   HP+F ++DW  L+ + +     PA                                 A P+ E QKYF +W ++S +TL+ E+GIA E   +   +K  Q+ G
Sbjct:   78 ELLQLLHDPVGQKHIGAFAKKIMTSETFFCWVEIKEFREAPSSGYRKSMAKLIYRKYIKNGSTMALGSITSDLITHYDKQIAELDTNHGASATLDFFDPLMANIVADMCQNTFIRFKASPE-YEAFKKDLKDTYNKVTIEDFEYLEVLGSGGFGRVVHARKKSTGKHYAMKIQLKTGLLDEHHDQLSQITSEKDILQICHNPFVLDMHYSFQTSAHAIIVTELVRGGDLSDALKACREGYIHEDRVRLYAAEIGLALNHMHELGLLYRDLKPGNVLLGEDGHVILADMGLATGFDPLKLQKE-ESGEFWYTAIQPKLT---RRRTTVGTRGYMAPEIVSGKLMKRNE--RQGYTYAIDFWSLGVTMFELLCGFRPFTSPSTGNYFQDLANDETYG---LSPRSQLKIE--------LERMAQCVTFPSHVSPNAQDFLKRLLEVLPENRLGCNDEKGFVEFIEHPFFETIDWEKLMMKHIDPTFKPAVQLLSDKRIFNSYEQMMSYFDTRDKDFSRYDWYADPDPEMQKYFDNWEFISLNTLRAELGIAKELDEMNIPYKVLQLTG 625          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig847.3.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3UU75_HETAK9.090e-17796.64Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
D7FP09_ECTSI5.100e-13341.33Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S1U415_9STRA1.910e-11440.94Hypothetical protein n=1 Tax=Phaeomonas parva TaxI... [more]
A0A835ZAC1_9STRA1.000e-11238.90Kinase-like domain-containing protein n=1 Tax=Trib... [more]
F0WFN3_9STRA3.420e-10536.67Protein kinase putative n=2 Tax=Albugo TaxID=65356... [more]
A0A8K1C8Y3_PYTOL2.630e-9936.45Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
K3X895_GLOUD6.420e-9936.33Uncharacterized protein n=1 Tax=Globisporangium ul... [more]
D8LEF7_ECTSI9.620e-9936.79Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
H3GJZ7_PHYRM3.130e-9535.54Uncharacterized protein n=20 Tax=Peronosporaceae T... [more]
A0A0P1B3D1_PLAHL9.500e-9435.34Agc protein kinase n=1 Tax=Plasmopara halstedii Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig847contigH-akashiwo_Contig847:394862..396641 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FP09
PFAMsPkinase,RGS
Model size1698
Max annot lvl2759|Eukaryota
KEGG koko:K00910,ko:K04688
KEGG Pathwayko01521,ko01522,ko04012,ko04062,ko04066,ko04140,ko04144,ko04150,ko04151,ko04152,ko04211,ko04212,ko04213,ko04340,ko04350,ko04371,ko04666,ko04714,ko04724,ko04740,ko04745,ko04910,ko04931,ko05032,ko05165,ko05200,ko05205,ko05210,ko05212,ko05221,ko05224,ko05225,ko05226,ko05231,map01521,map01522,map04012,map04062,map04066,map04140,map04144,map04150,map04151,map04152,map04211,map04212,map04213,map04340,map04350,map04371,map04666,map04714,map04724,map04740,map04745,map04910,map04931,map05032,map05165,map05200,map05205,map05210,map05212,map05221,map05224,map05225,map05226,map05231
Hectar predicted targeting categoryother localisation
Exons2
Evalue3.62e-78
EggNOG OGsKOG0598@1|root,KOG0598@2759|Eukaryota
EC2.7.11.1,2.7.11.15
Descriptionprotein serine/threonine kinase activity
Cds size672
COG categoryH
BRITEko00000,ko00001,ko01000,ko01001,ko04147
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388010.1436565-UTR-H-akashiwo_Contig847:394861..3953741681388010.1436565-UTR-H-akashiwo_Contig847:394861..395374Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig847 394862..395374 +
1681388010.1854491-UTR-H-akashiwo_Contig847:396128..3966411681388010.1854491-UTR-H-akashiwo_Contig847:396128..396641Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig847 396129..396641 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681388010.1600177-CDS-H-akashiwo_Contig847:395374..3958991681388010.1600177-CDS-H-akashiwo_Contig847:395374..395899Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig847 395375..395899 +
1681388010.1725876-CDS-H-akashiwo_Contig847:395981..3961281681388010.1725876-CDS-H-akashiwo_Contig847:395981..396128Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig847 395982..396128 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig847.3.1prot_H-akashiwo_Contig847.3.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig847 395375..396128 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig847.3.1

>prot_H-akashiwo_Contig847.3.1 ID=prot_H-akashiwo_Contig847.3.1|Name=mRNA_H-akashiwo_Contig847.3.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=224bp
MELLGAGAFGRVVHVRKKSTGNHYAMKIQIKRDLVANFRDDLTKLENEKT
VFAACNHPFILDMHYAFQTELYCVLVLRLVTAGNLQDAIDESALGRLGEE
QARFYTAEVALALFHLHDMGLMYRDLKPRNVLLNQDGHVLLADMGGVGDF
AGSVFSKTLKKANTKLTTSSKGLSTNWRRISIIGTKGYMAPEVVKLMSPA
DGPRKGTPRPWTTGRWAPPPTSC*
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mRNA from alignment at H-akashiwo_Contig847:394862..396641+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig847.3.1 ID=mRNA_H-akashiwo_Contig847.3.1|Name=mRNA_H-akashiwo_Contig847.3.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=1780bp|location=Sequence derived from alignment at H-akashiwo_Contig847:394862..396641+ (Heterosigma akashiwo CCMP452)
ACGACATGGACTAAAACAGCTGATCACCACGATTATCTCCAATCTGATTT GCTGCCAGACTGTGAAGATGTTACAGCTTACGCAGAACTGTTGGCAGTTC TCGATTCCCCTGTCGGTCAAAGGCATCTTGGTGTTTATGCAAAAAAGATA TTCTGCCAAGAGCTGCTGCTCTTTTGGACTGACTGCCAGGTCTATCGGAG TATTCCTACTTGTGACTATAGGCGAGCCAAAGGGGGTAATATTCACAACA AATACATAAAGTCCGGGGCAGTGCGGGAGCTAGGGTTCGTAACTGAGCAG GACAAAGCCAATTATGCCGAGCTCTTGGCAAGGGCGCGGGAGCAGCCACA GCTACTGGGTACTGAGCTCTACGACGACTTACTGAAGCGTTGCTTCCTTG AGATCTTTCAGCAGATTTTCATCCGCTTCAAAGTGGATGACGAGTCGTAT TCGGCCTTCCGGGAAGAGTCAGATGCAACCTATAATGTGGTGGGGGTTGA GGACTTTGAGTACATGGAGCTACTGGGCGCAGGTGCCTTTGGCAGGGTGG TGCATGTGCGGAAAAAATCCACGGGGAACCACTATGCCATGAAGATTCAG ATCAAGCGCGACCTGGTGGCCAACTTCCGGGACGACCTGACAAAGCTGGA AAACGAGAAGACCGTATTCGCGGCCTGCAACCACCCGTTCATCCTGGACA TGCACTATGCGTTCCAGACGGAGCTCTACTGCGTGCTGGTGCTGCGGCTG GTAACCGCGGGCAACCTGCAGGACGCAATCGACGAGAGCGCCCTGGGCCG GCTGGGGGAGGAGCAGGCGCGGTTCTACACGGCGGAGGTGGCCCTGGCCC TCTTCCACCTGCACGACATGGGCCTCATGTACCGGGACCTCAAGCCGCGC AACGTGCTGCTCAACCAGGATGGGCACGTGCTGCTGGCGGACATGGGCGG CGTGGGGGACTTCGCCGGCTCGGTGTTCAGCAAGACGCTAAAGAAGGCCA ACACCaAGCTGACGACCTCGTCCAAGGGCCTGTCCACCGTGAACGAGGAG AAGAGCGGCCGTGGGGGGGGGGGGGGTCGGGGCGGTTGGAGTCGGTGTCC ATGGCGGAAGGGAGGTGGAGAACTGGCGGCGCATCTCGATCATCGGCACC AAGGGGTACATGGCCCCAGAAGTGGTCAAGTTGATGAGCCCTGCGGACGG GCCGCGAAAGGGTACACCAAGACCGTGGACTACTGGTCGCTGGGCGCCAC CACCCACAAGCTGCTGACCGGGCGGCGGCCGCTCGAGCAACCCCAAGAGC CTTTTTCCACAGCAACCGCCACCAGCACCGCAGCGAGCACGAGAACCTTC TGAGCAGAAAGGTGGAGTACCCCAGCCACGCCAGCCCGAGGCCAAGAGCT TCATCAAAGAGTGCCTGGACGTGAACGAGGAGACGCGGATGGGGGGGCGG GGCGGGCGGCAGCGGCTGGAGAACCACCCGTGGTTTGCCTCGGTGGACTG GGCGGCGCTGCTGGCGCGCACGGTGCGGGTGCCGCACGTGCCGGCGGCCG TGCCGGCCGTGGAGACCCCGCTCTTCCCCAGCTTCCAGGCCATGATGGAC ACGCTCGACCAGGAGGACCGCCACCGGCGGCGGCGCAGCGTGCGGGCGCC CAACCGCGAGCAGCAGAAGTACTTCCGCAGCTGGAACTACGTCTCGCCGC ACACACTCAAGGTGGAGATGGGCATTGCCAACGAGAGCCGGGCGCTGACC ACCAACTTCAAGGCCCAGCAGATGATGGGG
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Coding sequence (CDS) from alignment at H-akashiwo_Contig847:394862..396641+

>mRNA_H-akashiwo_Contig847.3.1 ID=mRNA_H-akashiwo_Contig847.3.1|Name=mRNA_H-akashiwo_Contig847.3.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=672bp|location=Sequence derived from alignment at H-akashiwo_Contig847:394862..396641+ (Heterosigma akashiwo CCMP452)
ATGGAGCTACTGGGCGCAGGTGCCTTTGGCAGGGTGGTGCATGTGCGGAA
AAAATCCACGGGGAACCACTATGCCATGAAGATTCAGATCAAGCGCGACC
TGGTGGCCAACTTCCGGGACGACCTGACAAAGCTGGAAAACGAGAAGACC
GTATTCGCGGCCTGCAACCACCCGTTCATCCTGGACATGCACTATGCGTT
CCAGACGGAGCTCTACTGCGTGCTGGTGCTGCGGCTGGTAACCGCGGGCA
ACCTGCAGGACGCAATCGACGAGAGCGCCCTGGGCCGGCTGGGGGAGGAG
CAGGCGCGGTTCTACACGGCGGAGGTGGCCCTGGCCCTCTTCCACCTGCA
CGACATGGGCCTCATGTACCGGGACCTCAAGCCGCGCAACGTGCTGCTCA
ACCAGGATGGGCACGTGCTGCTGGCGGACATGGGCGGCGTGGGGGACTTC
GCCGGCTCGGTGTTCAGCAAGACGCTAAAGAAGGCCAACACCaAGCTGAC
GACCTCGTCCAAGGGCCTGTCCACCAACTGGCGGCGCATCTCGATCATCG
GCACCAAGGGGTACATGGCCCCAGAAGTGGTCAAGTTGATGAGCCCTGCG
GACGGGCCGCGAAAGGGTACACCAAGACCGTGGACTACTGGTCGCTGGGC
GCCACCACCCACAAGCTGCTGA
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