mRNA_H-akashiwo_Contig812.10.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig812.10.1
Unique NamemRNA_H-akashiwo_Contig812.10.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: A0A7S4DDF7_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DDF7_HETAK)

HSP 1 Score: 128 bits (321), Expect = 1.580e-34
Identity = 59/59 (100.00%), Postives = 59/59 (100.00%), Query Frame = 2
Query:    8 MDLVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLEM 184
            MDLVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLEM
Sbjct:    1 MDLVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLEM 59          
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: A0A6H5JGF2_9PHAE (CN hydrolase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JGF2_9PHAE)

HSP 1 Score: 108 bits (271), Expect = 8.730e-30
Identity = 47/56 (83.93%), Postives = 54/56 (96.43%), Query Frame = 2
Query:   14 LVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLE 181
            LVTVA CNL+QWALDFDGNLER+L+SIREAKA G++YRLGPELE+CGYGCEDH+LE
Sbjct:   11 LVTVAACNLDQWALDFDGNLERVLRSIREAKAMGSRYRLGPELELCGYGCEDHFLE 66          
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: A0A5D6XZ74_9STRA (Glutamine-dependent NAD(+) synthetase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XZ74_9STRA)

HSP 1 Score: 115 bits (289), Expect = 1.090e-28
Identity = 51/57 (89.47%), Postives = 55/57 (96.49%), Query Frame = 2
Query:   14 LVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLEM 184
            LVTVATCNLNQWALDFDGNLERI+ SIREAKA+GAKYRLGPELE+CGYGCEDH+LE 
Sbjct:    4 LVTVATCNLNQWALDFDGNLERIMTSIREAKARGAKYRLGPELEVCGYGCEDHFLEQ 60          
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: A0A6A4ZXR1_9STRA (CN hydrolase domain-containing protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A6A4ZXR1_9STRA)

HSP 1 Score: 108 bits (269), Expect = 2.800e-28
Identity = 49/56 (87.50%), Postives = 52/56 (92.86%), Query Frame = 2
Query:   14 LVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLE 181
            LVTVATCNLNQWALDFDGNL RI+QSIR AK +GA YRLGPELEICGYGCEDH+LE
Sbjct:    7 LVTVATCNLNQWALDFDGNLARIVQSIRIAKERGATYRLGPELEICGYGCEDHFLE 62          
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: A0A662WL89_9STRA (Glutamine-dependent NAD(+) synthetase n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662WL89_9STRA)

HSP 1 Score: 114 bits (285), Expect = 3.840e-28
Identity = 49/57 (85.96%), Postives = 56/57 (98.25%), Query Frame = 2
Query:   14 LVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLEM 184
            LVTVATCNLNQWALDFDGNLERIL+SIR+AKA+GA+YR+GPELE+CGYGCEDH+LE 
Sbjct:    4 LVTVATCNLNQWALDFDGNLERILESIRQAKARGARYRVGPELEVCGYGCEDHFLEQ 60          
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: A0A2D4BPM7_PYTIN (Glutamine-dependent NAD(+) synthetase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BPM7_PYTIN)

HSP 1 Score: 113 bits (282), Expect = 9.830e-28
Identity = 49/56 (87.50%), Postives = 55/56 (98.21%), Query Frame = 2
Query:   14 LVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLE 181
            LVT+ATCNLNQWALDFDGNLERIL+SIREAKA+GA YR+GPELE+CGYGCEDH+LE
Sbjct:    4 LVTLATCNLNQWALDFDGNLERILESIREAKARGATYRIGPELEVCGYGCEDHFLE 59          
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: G4YVY0_PHYSP (Glutamine-dependent NAD(+) synthetase n=2 Tax=Peronosporaceae TaxID=4777 RepID=G4YVY0_PHYSP)

HSP 1 Score: 112 bits (281), Expect = 1.340e-27
Identity = 49/57 (85.96%), Postives = 55/57 (96.49%), Query Frame = 2
Query:   14 LVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLEM 184
            LVTVATCNLNQWALDFDGNLERIL+SIR AKA+GA+YR+GPELE+CGYGCEDH+LE 
Sbjct:    4 LVTVATCNLNQWALDFDGNLERILESIRVAKARGARYRVGPELEVCGYGCEDHFLEQ 60          
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: A0A3R7H6U3_9STRA (NAD(+) synthase (glutamine-hydrolyzing) n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7H6U3_9STRA)

HSP 1 Score: 112 bits (281), Expect = 1.360e-27
Identity = 49/57 (85.96%), Postives = 55/57 (96.49%), Query Frame = 2
Query:   14 LVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLEM 184
            LVTVATCNLNQWALDFDGNLERIL+SIR+AKA GA+YR+GPELE+CGYGCEDH+LE 
Sbjct:  205 LVTVATCNLNQWALDFDGNLERILESIRQAKALGARYRVGPELEVCGYGCEDHFLEQ 261          
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: A0A7S0MSQ6_9CHLO (Hypothetical protein (Fragment) n=1 Tax=Pyramimonas obovata TaxID=1411642 RepID=A0A7S0MSQ6_9CHLO)

HSP 1 Score: 104 bits (260), Expect = 1.420e-27
Identity = 44/58 (75.86%), Postives = 54/58 (93.10%), Query Frame = 2
Query:    8 MDLVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLE 181
            M +VT+ATCNLNQWA+DFD N+ER+ +SIR+AKA GA+YR+GPELEICGYGCEDH+LE
Sbjct:    1 MRVVTLATCNLNQWAMDFDSNIERVEESIRQAKAVGARYRVGPELEICGYGCEDHFLE 58          
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Match: W7T1K7_9STRA (Glutamine-dependent NAD(+) synthetase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7T1K7_9STRA)

HSP 1 Score: 112 bits (279), Expect = 2.530e-27
Identity = 50/59 (84.75%), Postives = 54/59 (91.53%), Query Frame = 2
Query:    8 MDLVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGYGCEDHYLEM 184
            M L+TVATCNLNQWALDFDGNL+RI QSI +AKAKGA YRLGPELEICGYGCEDH+LE 
Sbjct:    1 MSLITVATCNLNQWALDFDGNLKRIKQSIFQAKAKGASYRLGPELEICGYGCEDHFLEQ 59          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig812.10.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S4DDF7_HETAK1.580e-34100.00Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A6H5JGF2_9PHAE8.730e-3083.93CN hydrolase domain-containing protein n=1 Tax=Ect... [more]
A0A5D6XZ74_9STRA1.090e-2889.47Glutamine-dependent NAD(+) synthetase n=1 Tax=Pyth... [more]
A0A6A4ZXR1_9STRA2.800e-2887.50CN hydrolase domain-containing protein n=1 Tax=Aph... [more]
A0A662WL89_9STRA3.840e-2885.96Glutamine-dependent NAD(+) synthetase n=1 Tax=Noth... [more]
A0A2D4BPM7_PYTIN9.830e-2887.50Glutamine-dependent NAD(+) synthetase n=1 Tax=Pyth... [more]
G4YVY0_PHYSP1.340e-2785.96Glutamine-dependent NAD(+) synthetase n=2 Tax=Pero... [more]
A0A3R7H6U3_9STRA1.360e-2785.96NAD(+) synthase (glutamine-hydrolyzing) n=3 Tax=Ph... [more]
A0A7S0MSQ6_9CHLO1.420e-2775.86Hypothetical protein (Fragment) n=1 Tax=Pyramimona... [more]
W7T1K7_9STRA2.530e-2784.75Glutamine-dependent NAD(+) synthetase n=2 Tax=Mono... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig812contigH-akashiwo_Contig812:483563..483746 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog36331.EPrPI00000019720
PFAMsCN_hydrolase,NAD_synthase
Model size185
Max annot lvl121069|Pythiales
Hectar predicted targeting categoryno signal peptide or anchor
Exons1
Evalue4.21e-29
EggNOG OGsCOG0171@1|root,KOG2303@2759|Eukaryota,1MJAT@121069|Pythiales
DescriptionGlutamine-dependent NAD( ) synthetase. Source PGD
Cds size177
COG categoryH
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387995.4191842-UTR-H-akashiwo_Contig812:483562..4835691681387995.4191842-UTR-H-akashiwo_Contig812:483562..483569Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig812 483563..483569 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387995.4311557-CDS-H-akashiwo_Contig812:483569..4837461681387995.4311557-CDS-H-akashiwo_Contig812:483569..483746Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig812 483570..483746 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig812.10.1prot_H-akashiwo_Contig812.10.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig812 483570..483746 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig812.10.1

>prot_H-akashiwo_Contig812.10.1 ID=prot_H-akashiwo_Contig812.10.1|Name=mRNA_H-akashiwo_Contig812.10.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=59bp
MDLVTVATCNLNQWALDFDGNLERILQSIREAKAKGAKYRLGPELEICGY
GCEDHYLEM
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mRNA from alignment at H-akashiwo_Contig812:483563..483746+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig812.10.1 ID=mRNA_H-akashiwo_Contig812.10.1|Name=mRNA_H-akashiwo_Contig812.10.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=184bp|location=Sequence derived from alignment at H-akashiwo_Contig812:483563..483746+ (Heterosigma akashiwo CCMP452)
CGGCATTATGGACCTTGTCACCGTAGCAACTTGCAACTTGAATCAATGGG CCCTTGACTTTGATGGGAACTTGGAGCGCATTCTACAATCCATCAGAGAA GCGAAAGCCAAGGGTGCAAAGTACCGCCTTGGACCAGAACTCGAAATCTG TGGGTATGGCTGTGAAGACCATTACCTCGAAATG
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Coding sequence (CDS) from alignment at H-akashiwo_Contig812:483563..483746+

>mRNA_H-akashiwo_Contig812.10.1 ID=mRNA_H-akashiwo_Contig812.10.1|Name=mRNA_H-akashiwo_Contig812.10.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=177bp|location=Sequence derived from alignment at H-akashiwo_Contig812:483563..483746+ (Heterosigma akashiwo CCMP452)
ATGGACCTTGTCACCGTAGCAACTTGCAACTTGAATCAATGGGCCCTTGA
CTTTGATGGGAACTTGGAGCGCATTCTACAATCCATCAGAGAAGCGAAAG
CCAAGGGTGCAAAGTACCGCCTTGGACCAGAACTCGAAATCTGTGGGTAT
GGCTGTGAAGACCATTACCTCGAAATG
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