mRNA_H-akashiwo_Contig1247.3.1 (mRNA) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: A0A7S4DBK0_HETAK (Phosphoenolpyruvate carboxykinase (ATP) n=4 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DBK0_HETAK)

HSP 1 Score: 840 bits (2169), Expect = 3.220e-304
Identity = 399/412 (96.84%), Postives = 403/412 (97.82%), Query Frame = 1

Query:  130 FVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRWPSRPPGLC 1365
            FVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSE+LVDWNNINQEASPEVW+DLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELV FEPDFTIINSCQTSNPKWKEHGLNS+VYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTI PETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKR   +    C
Sbjct:    1 FVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRAC 412          

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: A0A7S4DBG4_HETAK (Phosphoenolpyruvate carboxykinase (ATP) (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DBG4_HETAK)

HSP 1 Score: 704 bits (1816), Expect = 3.050e-253
Identity = 330/337 (97.92%), Postives = 335/337 (99.41%), Query Frame = 1

Query:  130 FVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGY 1140
            FVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSE+LVDWNNINQEASPEVW+DLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITE+AWQHHFVRNMFIRPTTEEELV FEP+FTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSR+LLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTI PETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGY
Sbjct:    1 FVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITEMAWQHHFVRNMFIRPTTEEELVGFEPEFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRFLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGY 337          

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: A0A7S2UVQ5_9STRA (Phosphoenolpyruvate carboxykinase (ATP) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UVQ5_9STRA)

HSP 1 Score: 710 bits (1832), Expect = 4.150e-252
Identity = 337/432 (78.01%), Postives = 374/432 (86.57%), Query Frame = 1

Query:   46 GITDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKG-PAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKR 1338
            GI  P  ++HNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDK+VV+N G D    VDWNNINQ  S E+W D+ AN++ +MNKAERLYV+D + G N  T+K +RF+ E AWQ HFV+NMFIRP+ +E L DF+PDF +INSC T NPKWKEHGLNSDVYA FNL+E +AVIGGTYYGGE KKGIFALMNY LP EGIMSMHCSANKG   GDT LFFGLSGTGKTTLSAD SRYL+GDDEHGWD+DGIFNFE GCYAKTI+L EENEP+I+RAI+PDALLENN IVPETNWPDFA+T+KTQNGRVSYP+YHIPN+EPSG   HP NC+FLTCDAFGVLPPV+RL   QAVYHFLSGYTAKVAGTERGI EP ATFSAGFGAAFLTLHPT YADLL +KL +HGTK WLVNTGWSGG+ GVG+R
Sbjct:   42 GIHTPTTIFHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKYVVKNEGADQ---VDWNNINQPISEEIWADMKANTLEYMNKAERLYVYDCFCGTNKKTRKAIRFLFEKAWQMHFVKNMFIRPSVDE-LADFKPDFQVINSCGTVNPKWKEHGLNSDVYAAFNLKENLAVIGGTYYGGENKKGIFALMNYTLPQEGIMSMHCSANKGMETGDTCLFFGLSGTGKTTLSADPSRYLIGDDEHGWDDDGIFNFEQGCYAKTINLSEENEPDIYRAIKPDALLENNFIVPETNWPDFANTSKTQNGRVSYPIYHIPNFEPSGSGTHPANCVFLTCDAFGVLPPVSRLSDEQAVYHFLSGYTAKVAGTERGINEPTATFSAGFGAAFLTLHPTKYADLLVQKLKKHGTKTWLVNTGWSGGSAGVGER 469          

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: W7TU57_9STRA (Phosphoenolpyruvate carboxykinase (ATP) n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TU57_9STRA)

HSP 1 Score: 651 bits (1679), Expect = 6.690e-229
Identity = 303/433 (69.98%), Postives = 362/433 (83.60%), Query Frame = 1

Query:   40 KWGITDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKR 1338
            K GIT+P  ++ NLSYP++A HE   KEG FV+NGTFAVDTG FTGRSPKDKW+V+     SE  + W ++NQ    EV+++LY   ++H NK ++ Y+FDGY GANP+++KKVRF+TE AWQ HFV NMFIRP + +E+  F PDFTIIN+C+  + KWKEHG+NS+V+  FNLE+KVA+IGGT+YGGEMKKGIF++MNYWLPLEGIM MHCSANKG AGDTALFFGLSGTGKTTLSAD  RYL+GDDEHGWD+ GIFNFEGGCYAKTI+L EENEP+I+RAI+ DA+LEN  I P T  PD+ +T+KT+NGRVSYPL+HI NYEP+ M  HPD  IFLTCDAFGVLPPVA+L   QA+YHFLSGYTAKVAGTERGITEP+ATFSA FGAAFLTLHPT YADLL++KL++HGT  +LVNTGW+GG YGVGKR
Sbjct:   37 KLGITNPTTIFRNLSYPDLAKHEEANKEGTFVANGTFAVDTGKFTGRSPKDKWIVKQ--APSEDNLWWGSVNQPMKGEVFEELYKKCVAHYNKLDKAYIFDGYCGANPSSRKKVRFLTEQAWQQHFVTNMFIRPQSTKEIEGFAPDFTIINACRVVDEKWKEHGINSEVFVAFNLEKKVAIIGGTFYGGEMKKGIFSMMNYWLPLEGIMPMHCSANKGKAGDTALFFGLSGTGKTTLSADPGRYLIGDDEHGWDDHGIFNFEGGCYAKTINLSEENEPDIYRAIKRDAMLENVYIDPTTKVPDYYNTSKTENGRVSYPLHHINNYEPTSMGDHPDKVIFLTCDAFGVLPPVAKLSPGQAMYHFLSGYTAKVAGTERGITEPQATFSACFGAAFLTLHPTKYADLLQKKLEKHGTATYLVNTGWTGGGYGVGKR 467          

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: A0A7R9UE17_9STRA (Phosphoenolpyruvate carboxykinase (ATP) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9UE17_9STRA)

HSP 1 Score: 637 bits (1644), Expect = 6.000e-223
Identity = 303/441 (68.71%), Postives = 359/441 (81.41%), Query Frame = 1

Query:   46 GITDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMN-KAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRWPSRPPGLC 1365
            G+T    +  N SY  +   E   KE    SNG  AVDTG FTGRSPKDKW+V+ P   SE  + W+++N+  +PEV+D+LYA  + H N  AE++YVFDG+ GANP T+K VRF+TE+AWQ+HFVRNMFIRP +++E+ +F PD+TIIN+C+ S+  WK+HGLNSDV+  FNLE+KVA+IGGTYYGGEMKKGIF++MNYWLPLEGIMSMHCSANKG  GDTALFFGLSGTGKT LSAD +RYL+GDDEHGWD DGIFNFEGGCYAKTIDL EENEPEI+RAI+ DALLEN T+    N PD+ +T+KTQNGRVSYPL+HI NY+P  M GHP+N IFLTCDAFG+LPPV++L   QA+YHFLSGYTAKVAGTERGI EP+ATFSA FGAAFLTLHPT YADLL+EKL +HGTKA+LVNTGWSGG YGVG R P +    C
Sbjct:   80 GLTTEVPMMRNPSYETLFEEEMANKEAIATSNGAMAVDTGKFTGRSPKDKWIVKQPP--SEGNLWWSDVNRGITPEVFDELYAKVLDHYNYDAEKVYVFDGFCGANPTTRKNVRFVTEMAWQNHFVRNMFIRPKSDDEIANFAPDYTIINACRISDEDWKKHGLNSDVFVAFNLEKKVAIIGGTYYGGEMKKGIFSMMNYWLPLEGIMSMHCSANKGKNGDTALFFGLSGTGKTALSADPNRYLIGDDEHGWDQDGIFNFEGGCYAKTIDLTEENEPEIYRAIKRDALLENVTLEGPNNTPDYFNTSKTQNGRVSYPLHHIENYDPVSMGGHPNNMIFLTCDAFGILPPVSKLTPEQAMYHFLSGYTAKVAGTERGIKEPQATFSACFGAAFLTLHPTKYADLLQEKLQRHGTKAYLVNTGWSGGMYGVGSRMPIKATRAC 518          

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: H3G8U5_PHYRM (Phosphoenolpyruvate carboxykinase (ATP) n=24 Tax=Peronosporaceae TaxID=4777 RepID=H3G8U5_PHYRM)

HSP 1 Score: 630 bits (1626), Expect = 1.990e-221
Identity = 291/435 (66.90%), Postives = 352/435 (80.92%), Query Frame = 1

Query:   61 RIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRWPSRPPGLC 1365
            R ++ NLSY +IA HE    EG FV NGT+ +DTG FTGRSPKDK++V      S + + W +INQ  S E++D+LY     H   AE++YVFDGY GANPA++KKVRFITELAWQHHFV NMF+RP TEEE+ DF+PDFTI+N+C+ +N  +K+HGLNS+V+  FN+E+ VAVIGGT+YGGEMKKGIF++MNYWLPL+GIM+MHCSANKG  GDTALFFGLSGTGKTTLSAD  RYL+GDDEHGWD+DGIFNFEGGCYAKTI+L  ENEP+I+ AI+ DALLEN  +  +T  PDF +T+KT+NGRVSYP+YHIPN+EP+   GHP N +FLTCDA+GVLPPV++L   QA+YHFLSGYTAKVAGTERG+TEP ATFSA FGAAFL LHPT YADLL++KL +H T  +LVNTGW+ G YGVGKR   +    C
Sbjct:    7 RAMHRNLSYDDIAAHEEKNGEGQFVKNGTYTIDTGKFTGRSPKDKYIVDQ--APSSKNIWWGDINQPVSSEIFDELYETVTKHYGSAEKVYVFDGYAGANPASRKKVRFITELAWQHHFVTNMFLRPQTEEEIADFKPDFTIVNACKVTNQDYKKHGLNSEVFVAFNIEKDVAVIGGTWYGGEMKKGIFSMMNYWLPLDGIMAMHCSANKGKKGDTALFFGLSGTGKTTLSADPHRYLIGDDEHGWDDDGIFNFEGGCYAKTINLSAENEPDIYNAIKRDALLENTFVDADTKEPDFYNTSKTENGRVSYPIYHIPNHEPTSSGGHPSNIVFLTCDAYGVLPPVSKLSDGQAMYHFLSGYTAKVAGTERGVTEPTATFSACFGAAFLPLHPTKYADLLQKKLQKHNTSVYLVNTGWTSGGYGVGKRMSIKDTRAC 439          

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: A0A7S1XX74_9STRA (Phosphoenolpyruvate carboxykinase (ATP) (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1XX74_9STRA)

HSP 1 Score: 626 bits (1614), Expect = 2.940e-220
Identity = 301/441 (68.25%), Postives = 358/441 (81.18%), Query Frame = 1

Query:   16 LEQLGIAQKWGITDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMNK-AERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGK 1335
            LE LGI      TD  ++  N SY E+   E    EG   S G+FA+DTG FTGRSPKDKW+V+     SE  + W ++N+   PEV+D+LY   ++H N   E++YVFDG+ GANP+T+K VRF+TE+AWQ+HFVRNMF+RP  E+E+ DF PDFTIIN+C+ S+  W+ HGLNSDV+  FNLE+K+A+IGGTYYGGEMKKGIF++MNYWLPLEGIM+MHCSANKG  GDTALFFGLSGTGKTTLSAD +RYL+GDDEHGWD+DGIFNFEGGCYAKTI+LKEENEPEI+RAI+ DALLEN  +  E N PD+ + TKTQNGRVSYPL+HI N++P  M GHP+N IFLTCDAFGVLPPV+RL   QA+YHFLSGYTAKVAGTERGI EPEATFSA FGAAFLTLHPT YADLL++KL+ H TK +LVNTGWSGGAYG G+
Sbjct:   46 LESLGID-----TDAPMM-RNPSYEELFEEEVANGEGVVTSTGSFAIDTGKFTGRSPKDKWIVKQ--APSEGNLWWGDVNRGIEPEVFDELYEKVVAHYNNDVEKVYVFDGFCGANPSTRKNVRFVTEMAWQNHFVRNMFLRPKNEDEIADFAPDFTIINACKISDQDWERHGLNSDVFVAFNLEKKIAIIGGTYYGGEMKKGIFSMMNYWLPLEGIMAMHCSANKGKNGDTALFFGLSGTGKTTLSADPNRYLIGDDEHGWDDDGIFNFEGGCYAKTINLKEENEPEIYRAIKRDALLENCWLEGEDNTPDYFNATKTQNGRVSYPLHHIENHDPVSMGGHPENIIFLTCDAFGVLPPVSRLTPEQAMYHFLSGYTAKVAGTERGIDEPEATFSACFGAAFLTLHPTKYADLLQKKLENHDTKVFLVNTGWSGGAYGTGE 478          

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: K3XBX7_GLOUD (Phosphoenolpyruvate carboxykinase (ATP) n=2 Tax=Pythiaceae TaxID=4782 RepID=K3XBX7_GLOUD)

HSP 1 Score: 629 bits (1623), Expect = 3.460e-220
Identity = 295/453 (65.12%), Postives = 363/453 (80.13%), Query Frame = 1

Query:   10 SRLEQLGIAQKWGITDPRI-VYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRWPSRPPGLC 1365
            +R   LG+  K+G+T+    V+HNLSY +IA HE   KEG F++NGT+ +DTG FTGRSPKDK++V      S + + W +IN+  +PEV+D LY     H   AE++YVFDGY GANP ++KKVR ITELAWQHHFV NMF+RP T+EE+ +FEPDFTI+N+C+ +N  +KEHGLNS+V+  FN+E+ VAVIGGT+YGGEMKKGIF++MNYWLPLEGIM+MHCSANKG  GDTALFFGLSGTGKTTLSAD  R+L+GDDEHGWD++GIFNFEGGCYAKTI+L +ENEP+I+ AI+ DALLEN  ++PE        T+KT+NGRVSYP+YHIPN+EP+   GHPDN +FLTCDA+GVLPPV++L   QA+YHFLSGYTAKVAGTERG+TEP ATFSA FGAAFL LHPT YADLL++KL +H T  +LVNTGW+GG YGVGKR   +    C
Sbjct:   41 ARAAALGL-DKFGVTNANTKVHHNLSYADIAKHEDANKEGQFIANGTYTIDTGKFTGRSPKDKYIVDQ--APSNKNIWWGDINRPITPEVFDALYDKVTGHYGTAEKVYVFDGYAGANPNSRKKVRIITELAWQHHFVTNMFLRPETKEEIENFEPDFTIVNACKVTNEDYKEHGLNSEVFVAFNIEKNVAVIGGTWYGGEMKKGIFSMMNYWLPLEGIMAMHCSANKGKNGDTALFFGLSGTGKTTLSADPHRWLIGDDEHGWDDEGIFNFEGGCYAKTINLSQENEPDIYNAIKRDALLENTFVIPEXXXXXXXXTSKTENGRVSYPIYHIPNHEPTSSGGHPDNIVFLTCDAYGVLPPVSKLSDGQAMYHFLSGYTAKVAGTERGVTEPTATFSACFGAAFLPLHPTRYADLLQQKLQKHKTSVYLVNTGWTGGGYGVGKRMSIKDTRAC 490          

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: A0A3M6VGM8_9STRA (Phosphoenolpyruvate carboxykinase (ATP) n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VGM8_9STRA)

HSP 1 Score: 624 bits (1608), Expect = 6.080e-218
Identity = 291/439 (66.29%), Postives = 360/439 (82.00%), Query Frame = 1

Query:   25 LGIAQKWGITDPRI-VYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKR 1338
            LGI +K+G+T+ +  V+ NLSY EIA HE    EG FV NGT+ +DTG FTGRSPKDK++V    +++   + W +IN+  S EV+D+LY     H +  E++YVFDGY GANPA++KKVRFITELAWQHHFV NMF+RP ++EE+ DF+PDFTI+N+C+ +N  +K+HGLNS+V+  FN+E+ VAVIGGT+YGGEMKKGIF++MNYWLPL+G+M+MHCSANKG  GDTALFFGLSGTGKTTLSAD  R L+GDDEHGWD+DGIFNFEGGCYAKTI+L  ENEP+I+ AI+ +ALLEN  +  ET  PDF +T+KT+NGRVSYP+YHIPN+EPS   GHP N +FLTCDA+GVLPPV++L   QA+YHFLSGYTAKVAGTERG+TEP ATFSA FGAAFL LHPT YADLL++KL +H T  +LVNTGW+ G YGVG+R
Sbjct:   44 LGI-EKFGVTNAKTQVHRNLSYDEIAAHEKKNGEGEFVKNGTYTIDTGKFTGRSPKDKYIVDQAPSNTN--IWWGDINRPVSAEVFDELYKTVTKHYSSTEKVYVFDGYAGANPASRKKVRFITELAWQHHFVTNMFLRPQSKEEIADFKPDFTIVNACKVTNKDYKKHGLNSEVFVAFNIEKDVAVIGGTWYGGEMKKGIFSMMNYWLPLDGVMAMHCSANKGKNGDTALFFGLSGTGKTTLSADPHRNLIGDDEHGWDDDGIFNFEGGCYAKTINLSRENEPDIYNAIKRNALLENVFVDAETKEPDFYNTSKTENGRVSYPIYHIPNHEPSSTGGHPSNIVFLTCDAYGVLPPVSKLSDGQAMYHFLSGYTAKVAGTERGVTEPTATFSACFGAAFLPLHPTKYADLLQQKLKKHNTSVYLVNTGWTSGGYGVGRR 479          

BLAST of mRNA_H-akashiwo_Contig1247.3.1 vs. uniprot
Match: A0A097IUF2_9PHAE (Phosphoenolpyruvate carboxykinase (ATP) n=1 Tax=Desmarestia viridis TaxID=62313 RepID=A0A097IUF2_9PHAE)

HSP 1 Score: 624 bits (1608), Expect = 6.520e-218
Identity = 293/456 (64.25%), Postives = 358/456 (78.51%), Query Frame = 1

Query:    1 ATASRLEQLGIAQKWGITDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSERLVDWNNINQEASPEVWDDLYANSISHMN-KAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVDFEPDFTIINSCQTSNPKWKEHGLNSDVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIVPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRWPSRPPGLC 1365
            +T + +  LGI +  GIT P  VY NL YPE+  HE   KEG    NGT+ V TG FTGRSPKDK+ V+     S++ +DW  +N   +PEV+ ++YA ++ H N   +++YVFDGY G +P ++K VR ITELAWQHHF +NMFIRP +E E+ DF+PDFTIIN+C+  N  +KE GLNSDV   FN+EE  AVI GT+YGGEMKKGIF++MNYWLPL G+M+MHCSANKG  GDTALFFGLSGTGKTTLSAD +RYL+GDDEHGWD+DGIFNFEGGCYAKT++L  ENEPEI+ AI+ DALLEN  I+ +   PD+AD ++T+N RVSYP+YHI NYEPSGM GHP++ IFLTCDAFGVLPPV+RL + QA+YHFLSGYTAKVAGTERG+TEP   FSAGFGAAFLTLHPT+YADLL++KL  HGT+ +L+NTGW+GG YGVGKR   +    C
Sbjct:   39 STMATVSSLGIDKATGITKPTTVYRNLPYPEVFEHETKNKEGEVTKNGTYCVSTGQFTGRSPKDKYFVQQ--APSDKNLDWGKVNVGVAPEVYKNIYAKAVKHYNDNVDKMYVFDGYCGTSPKSRKNVRIITELAWQHHFAKNMFIRPESEAEVADFKPDFTIINACKVVNDDYKELGLNSDVAVVFNVEENKAVITGTWYGGEMKKGIFSMMNYWLPLNGVMAMHCSANKGKNGDTALFFGLSGTGKTTLSADPARYLIGDDEHGWDDDGIFNFEGGCYAKTVNLSHENEPEIYDAIKRDALLEN-VIIKDNGEPDYADVSRTENTRVSYPIYHIDNYEPSGMGGHPNSIIFLTCDAFGVLPPVSRLSAGQAMYHFLSGYTAKVAGTERGVTEPTPNFSAGFGAAFLTLHPTMYADLLQKKLATHGTQCFLINTGWTGGGYGVGKRMSIKATRAC 491