mRNA_H-akashiwo_Contig1234.3.1 (mRNA) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: A0A6V1T6U9_HETAK (3-isopropylmalate dehydrogenase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1T6U9_HETAK)

HSP 1 Score: 791 bits (2044), Expect = 4.310e-289
Identity = 404/404 (100.00%), Postives = 404/404 (100.00%), Query Frame = 1

Query:    1 MIYSILFILAILQIANAFVGTPLMGARGSARTFMAVEKKDSYDITLLTGDGIGPEITQATVAALAPLEKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEESKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDICRTVDIAGGGLPTVGCKQMGEEVAKLVAALVEKKLVA 1212
            MIYSILFILAILQIANAFVGTPLMGARGSARTFMAVEKKDSYDITLLTGDGIGPEITQATVAALAPLEKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEESKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDICRTVDIAGGGLPTVGCKQMGEEVAKLVAALVEKKLVA
Sbjct:    1 MIYSILFILAILQIANAFVGTPLMGARGSARTFMAVEKKDSYDITLLTGDGIGPEITQATVAALAPLEKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEESKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDICRTVDIAGGGLPTVGCKQMGEEVAKLVAALVEKKLVA 404          

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: A0A7S2V595_9STRA (3-isopropylmalate dehydrogenase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V595_9STRA)

HSP 1 Score: 591 bits (1524), Expect = 6.840e-210
Identity = 298/375 (79.47%), Postives = 327/375 (87.20%), Query Frame = 1

Query:   76 ARGSARTFMAVEKKDSYDITLLTGDGIGPEITQATVAALAPLEKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTET-RDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEESKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDICRTVDIAGGGLPTVGCKQMGEEVAKLVAALVE 1197
            A G ++  MAV KK SYD+TLL GDGIGPEIT A + A+ PLE++GF MNFK+ALIGG A+D VN PFPDESL+QC+ SDSVLL+CIGGYKWDKNPR LRPETGLL+MRKEM LFANLRPAKV+PQL+GASTLK EVLEGVDIMVVRELTGDVYFG PKGT T  DG+RV YNNMIY E EI RIAKVAFDVAMKRGK V+SIDKANVLDVSQLWRDVV EES+NYPEV+L HM +DNAAMQLIRWPKQFDT+VCGNLFGDI+SDEASMLVGSLGMLPSASIG+P  PGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDL+R E A +LE AVE+VLD  RT DIA  G   +GCK+MGEEVAKLV   V+
Sbjct:   17 AYGFSQITMAVGKKASYDVTLLKGDGIGPEITDAAIVAMKPLEQMGFTMNFKDALIGGIAIDEVNDPFPDESLKQCQESDSVLLACIGGYKWDKNPRHLRPETGLLKMRKEMDLFANLRPAKVLPQLLGASTLKPEVLEGVDIMVVRELTGDVYFGQPKGTMTDEDGQRVGYNNMIYSEGEITRIAKVAFDVAMKRGKKVHSIDKANVLDVSQLWRDVVTEESQNYPEVDLQHMLVDNAAMQLIRWPKQFDTVVCGNLFGDILSDEASMLVGSLGMLPSASIGNPENPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLARPEAADILEAAVEEVLDTYRTGDIAADGFELIGCKKMGEEVAKLVEKRVK 391          

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: B7G1X8_PHATC (3-isopropylmalate dehydrogenase n=2 Tax=Naviculales TaxID=38748 RepID=B7G1X8_PHATC)

HSP 1 Score: 550 bits (1416), Expect = 2.820e-193
Identity = 290/399 (72.68%), Postives = 332/399 (83.21%), Query Frame = 1

Query:   10 SILFILAILQIANAFVGTPLMGARGSARTFMAVEKKDSYDITLLTGDGIGPEITQATVAALAPL-EKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEE-SKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDI-CRTVDIA--GGGLPTVGCKQMGEEVAKLVAAL 1191
            +++ +L +  +++AFV      AR  +    AV KKDSYDITLL GDGIGPEIT++T   L  L EK GF MN K ALIGGAA+DAVN PFP ESLEQC+ SDSVLL+CIGGYKWD NPRELRPE+GLL+MRKEMGLFANLRPAKV+PQL+ ASTLK EV+EGVD+MVVRELTGDVYFGTPKG ET +G+RV YNNMIY E EI RIA+VA DVA KRGK + S+DKANVLDVSQLWR+VV +  +K++P+VEL+HMY+DNAAMQLIRWPKQFDTIVCGN+FGDI+SDEASMLVGSLGMLPSASIG+ SGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMML+YDL R +EA +LE+AVE VLD   RT DI   G G   VGC +MGE VAK+V  L
Sbjct:   10 ALIVVLGVASLSDAFVPQHSRFARHPS-VKDAVSKKDSYDITLLPGDGIGPEITESTKKVLTALCEKCGFDMNLKVALIGGAAIDAVNDPFPQESLEQCQASDSVLLACIGGYKWDSNPRELRPESGLLKMRKEMGLFANLRPAKVLPQLIDASTLKREVVEGVDVMVVRELTGDVYFGTPKGIETINGERVGYNNMIYSESEIDRIARVAGDVASKRGKKLCSVDKANVLDVSQLWREVVTDVITKDFPDVELSHMYVDNAAMQLIRWPKQFDTIVCGNIFGDILSDEASMLVGSLGMLPSASIGE-SGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLKYDLDRPDEALLLENAVEAVLDQGLRTPDIKQEGDGCKLVGCSEMGEAVAKIVTEL 406          

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: A0A7S2P3R3_9STRA (3-isopropylmalate dehydrogenase n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2P3R3_9STRA)

HSP 1 Score: 535 bits (1379), Expect = 4.680e-188
Identity = 279/380 (73.42%), Postives = 314/380 (82.63%), Query Frame = 1

Query:   70 MGARGSARTFM-AVEKKDSYDITLLTGDGIGPEITQATVAALAPL-EKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEE-SKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDI-CRTVDIA--GGGLPTVGCKQMGEEVAKLVAAL 1191
            M  RGSA + + A  KKDSYDITLL GDGIGPEIT AT   L  L E+  FK+N KEALIGGAA+DA N PFPDESL+QC   DSVLL+CIGGYKWD NPRELRPETGLL+MRKEMGLFANLRPA V+ QL+ ASTLK EV+EGVD+MVVRELTGDVYFGTPKG +  DG+RV YNNMIY E EI+RIA+VA DVA KRG  + S+DKANVLDVSQLWR+VV +   K +P+VEL+HMY+DNAAMQLIRWPKQFDTIVCGN+FGDI+SDEASMLVGSLGMLPSASIG+  GPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDL R  EA +LE AVE+ L+   RT DI   G G   +GC +MGE+VA +V A+
Sbjct:    1 MSTRGSAISSLDATSKKDSYDITLLPGDGIGPEITTATTKVLQTLTERFNFKLNLKEALIGGAAIDAKNDPFPDESLQQCLSGDSVLLACIGGYKWDNNPRELRPETGLLKMRKEMGLFANLRPAVVLDQLIDASTLKREVVEGVDVMVVRELTGDVYFGTPKGIDVVDGERVGYNNMIYSESEIKRIARVAGDVASKRGGKLCSVDKANVLDVSQLWREVVTDICEKEFPDVELSHMYVDNAAMQLIRWPKQFDTIVCGNIFGDILSDEASMLVGSLGMLPSASIGE-DGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLDRPHEALVLEKAVEEALEQGLRTADIKQEGDGCKLIGCVEMGEQVANIVKAI 379          

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: A0A1E7F9J8_9STRA (3-isopropylmalate dehydrogenase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7F9J8_9STRA)

HSP 1 Score: 532 bits (1370), Expect = 2.770e-186
Identity = 283/390 (72.56%), Postives = 318/390 (81.54%), Query Frame = 1

Query:   37 QIANAFVGTPLMGARGSARTFMAVEKKDSYDITLLTGDGIGPEITQATVAALAPL-EKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEE-SKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDI-CRTVDIA--GGGLPTVGCKQMGEEVAKLVAAL 1191
            +IA+A   T       SA    AV KKDSY ITLL GDGIGPEIT AT  AL  L EK GF +  KEALIGGAA+DAVN PFP+ESL+QC  SDSVLL+CIGGYKWD NPRELRPE+GLL+MRKEMGLFANLRPAKV+PQLM ASTLK E++EGVDIM VRELTGDVYFGTPKG +  DG+RV YNNMIY E EI RIA+VA D+A KRG  + SIDKANVLDVSQLWR+VV +  +K++P VEL+HMY+DN AMQLIR+PKQFD IVCGN+FGDI+SDEASMLVGSLGMLPSASIG+ SGPGVFEPCHGSAPDIAG+DKANPLAMILSAAMML+YDL R EEA+MLEDAVE VLD   RT DI     G   VGC  MGE VA+++ A+
Sbjct:   25 KIADAVANTLSRSTTPSALN--AVSKKDSYSITLLPGDGIGPEITAATKVALTALCEKSGFTLELKEALIGGAAIDAVNDPFPEESLQQCLSSDSVLLACIGGYKWDNNPRELRPESGLLKMRKEMGLFANLRPAKVLPQLMDASTLKREIIEGVDIMCVRELTGDVYFGTPKGIDVIDGQRVGYNNMIYSEMEIDRIARVAGDIAAKRGGKLCSIDKANVLDVSQLWREVVTDVITKDFPSVELSHMYVDNCAMQLIRYPKQFDVIVCGNIFGDILSDEASMLVGSLGMLPSASIGE-SGPGVFEPCHGSAPDIAGDDKANPLAMILSAAMMLKYDLDRPEEAKMLEDAVEAVLDKGFRTPDIKIEDDGCQLVGCVAMGEAVAEVITAM 411          

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: A0A1Z5JD77_FISSO (3-isopropylmalate dehydrogenase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JD77_FISSO)

HSP 1 Score: 530 bits (1366), Expect = 9.070e-186
Identity = 282/387 (72.87%), Postives = 319/387 (82.43%), Query Frame = 1

Query:   49 AFVGTPLMGARGSARTFM-AVEKKDSYDITLLTGDGIGPEITQATVAALAPLE-KLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEE-SKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDI-CRTVDIA--GGGLPTVGCKQMGEEVAKLVAAL 1191
            AFV  P +    SA + + AV KKDSY +TLL GDGIGPEIT AT   L  L  +  F++ +KEALIGGAA+DA N PFP ESLEQC  SDSVLL+CIGGYKWDKNPRELRPETGLL+MRKEMGLFANLRPAKV+PQL+ ASTLK EV+EGVD+MVVRELTGDVYFGTPKG +  +G+RV YNNMIY E EI RIA+VA DVA KRG  + S+DKANVLDVSQLWRDVV +  +K++PEVEL+HMY+DNAAMQLIRWPKQFDTIVCGN+FGDI+SDEASMLVGSLGMLPSASIG+ SGPGVFEPCHGSAPDIAG+DKANPLAMILSAAMML+YDL R EEA +LE AVE  LD   RT D+     G   VGC +MG+ VAKLVA +
Sbjct:   21 AFV--PSVQRSSSASSLLGAVAKKDSYAVTLLPGDGIGPEITAATKQVLQALATRCNFRIEWKEALIGGAAIDAKNDPFPQESLEQCLASDSVLLACIGGYKWDKNPRELRPETGLLKMRKEMGLFANLRPAKVLPQLIDASTLKREVVEGVDVMVVRELTGDVYFGTPKGIDMVNGQRVGYNNMIYSESEIDRIARVAGDVASKRGGKLCSVDKANVLDVSQLWRDVVTDVITKDFPEVELSHMYVDNAAMQLIRWPKQFDTIVCGNIFGDILSDEASMLVGSLGMLPSASIGE-SGPGVFEPCHGSAPDIAGQDKANPLAMILSAAMMLQYDLDRPEEAALLEKAVEAALDAGVRTPDLKQDNDGCKLVGCAEMGQVVAKLVAEI 404          

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: B8C2I2_THAPS (3-isopropylmalate dehydrogenase n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C2I2_THAPS)

HSP 1 Score: 530 bits (1364), Expect = 2.110e-185
Identity = 278/390 (71.28%), Postives = 315/390 (80.77%), Query Frame = 1

Query:   43 ANAFVGTPLMGARGSARTFMAVEKKDSYDITLLTGDGIGPEITQATVAALAPLE-KLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEE-SKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDI-CRTVDIA--GGGLPTVGCKQMGEEVAKLVAALVE 1197
            ++  +  P  G   +     AV KKD+Y ITLL GDGIGPEI  AT   LA L+ + GF +   EALIGGAA+DAVN PFPDESL+QC  SDSVLLSCIGGYKWD NPRELRPE+GLL+MRK MGLFANLRPAKV+ QL+ ASTLK EV+EGVD+MVVRELTGDVYFGTPKG +  DGKR+ YNNMIY E+EI RIA+VA DVA KRG  + S+DKANVLDVSQLWRDV  +  +K +P+VEL+HMY+DNAAMQLIRWPKQFDT+V GN+FGDI+SDEASMLVGSLGMLPSASIG+ SGPGVFEPCHGSAPDIAGEDKANPLA+ILSAAMMLRYDL RAEEA +LE AVE VLD   RT DI   G     VGC +MG+ VA +VAA VE
Sbjct:   25 SSTLLSPPSAGTTTAVAPLAAVSKKDTYQITLLPGDGIGPEIMTATKQVLASLQSRCGFTLEMTEALIGGAAIDAVNDPFPDESLQQCLKSDSVLLSCIGGYKWDSNPRELRPESGLLKMRKSMGLFANLRPAKVLDQLIDASTLKREVVEGVDVMVVRELTGDVYFGTPKGIDVVDGKRIGYNNMIYSEDEIVRIARVAGDVASKRGGKLCSVDKANVLDVSQLWRDVTTDIITKEFPDVELSHMYVDNAAMQLIRWPKQFDTMVTGNIFGDILSDEASMLVGSLGMLPSASIGE-SGPGVFEPCHGSAPDIAGEDKANPLALILSAAMMLRYDLDRAEEADILERAVEAVLDQGLRTADIKQEGDNCKLVGCIEMGKAVADIVAAAVE 413          

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: A0A835ZK29_9STRA (3-isopropylmalate dehydrogenase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZK29_9STRA)

HSP 1 Score: 529 bits (1362), Expect = 3.190e-185
Identity = 276/374 (73.80%), Postives = 306/374 (81.82%), Query Frame = 1

Query:   82 GSARTFMAVEKKDSYDITLLTGDGIGPEITQATVAALAPLEKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRD-GKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEESKN-YPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDI-CRTVDIAGGGLPTVGCKQMGEEVAKLVAALV 1194
            GS    MAV KKDSY ITLL GDGIGPEIT ATVAA+ P  KL FKM FKEALIGGAA+DA   PFPD + EQ K SDSVLLS IGG KWD NPRE RPETGLL MRK +GLFANLRPAKV+ QL+ ASTLK E+++GVDIMVVRELTGDVYFGTPKG ET + G+R  YNNMIY E E+ RIA+VAFDVA KRGK V S+DKANVLDVSQLWRDVVI   K+ YP+VEL H Y+DNAAMQLIRWPKQFD +V GN+FGDI+SDEASMLVGSLGMLPSAS+G+PSGPGVFEPCHGSAPDIAG+DKANPLAMILSAAMMLRYDL R EEA +LE AV+ VLD    T DI   G   VGCK+MG+ VA+ +  L+
Sbjct:   34 GSRGLHMAVTKKDSYSITLLPGDGIGPEITTATVAAIQPFSKL-FKMEFKEALIGGAAIDATGSPFPDATFEQIKASDSVLLSAIGGPKWDGNPREQRPETGLLAMRKGLGLFANLRPAKVVRQLLDASTLKPEIVDGVDIMVVRELTGDVYFGTPKGIETTESGERFGYNNMIYYEHEVERIARVAFDVARKRGKRVCSVDKANVLDVSQLWRDVVIRIQKDEYPDVELTHQYVDNAAMQLIRWPKQFDVMVTGNIFGDILSDEASMLVGSLGMLPSASVGNPSGPGVFEPCHGSAPDIAGQDKANPLAMILSAAMMLRYDLGRPEEADLLETAVDNVLDQGYATADIMRDGCKPVGCKEMGQRVAEALEKLL 406          

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: D7G282_ECTSI (3-isopropylmalate dehydrogenase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G282_ECTSI)

HSP 1 Score: 529 bits (1363), Expect = 4.750e-185
Identity = 278/397 (70.03%), Postives = 321/397 (80.86%), Query Frame = 1

Query:   31 ILQIANAFVGTPLMGARG-----SARTFMAVEKKDSYDITLLTGDGIGPEITQATVAALAPLEKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGT-ETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEESKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDI-CRTVDIAGGGLPTVGCKQMG----EEVAKLVAA 1188
            +++ A AFVG+P++ AR      S +  M  E KDSYD+ LL GDGIGPEIT ATV AL PL+ L FK+NFKEALIGGAA+DA   P+PD +L   K SDS+LL+ IGG KWD NPRELRPETGLL MR ++GLFANLRPAKV+ +L+ AS+LK E++EGVDIMVVRELTGDVYFGTPKG  E   G+RV YNNMIY E E+ RIAKVA+DVAMKRGK + S+DKANVLDVSQLWRDVVI+ S++YPEVEL H Y+DNAAMQLIRWPKQFD +V GN+FGDI+SDEASMLVGSLGMLPSAS+G+P GPGVFEPCHGSAPDIAG+DKANPLAMILSAAMMLRYDL R  EA M+E AV+ V+D    T DI   G   VGCK+MG    EE+AKLVAA
Sbjct:   16 LIEQAQAFVGSPVLRARAFSPSTSHKLSMVTEAKDSYDVMLLPGDGIGPEITAATVNALEPLQSL-FKLNFKEALIGGAAIDAEGSPWPDSTLALAKASDSILLAAIGGPKWDGNPRELRPETGLLAMRSQLGLFANLRPAKVMKELVDASSLKPEIVEGVDIMVVRELTGDVYFGTPKGIDELPSGERVGYNNMIYHEHEVERIAKVAYDVAMKRGKRLCSVDKANVLDVSQLWRDVVIKMSEDYPEVELTHQYVDNAAMQLIRWPKQFDVMVTGNIFGDILSDEASMLVGSLGMLPSASMGNPDGPGVFEPCHGSAPDIAGQDKANPLAMILSAAMMLRYDLGRPAEADMVEAAVDVVMDKGYATNDIQREGTTAVGCKEMGDKVKEELAKLVAA 411          

BLAST of mRNA_H-akashiwo_Contig1234.3.1 vs. uniprot
Match: A0A6S8RM19_9STRA (3-isopropylmalate dehydrogenase n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6S8RM19_9STRA)

HSP 1 Score: 524 bits (1350), Expect = 2.830e-183
Identity = 283/410 (69.02%), Postives = 321/410 (78.29%), Query Frame = 1

Query:    1 MIYSILFILAILQIANAFVGTPLMGARGSARTF-MAVEKKDSYDITLLTGDGIGPEITQATVAALAPL-EKLGFKMNFKEALIGGAALDAVNHPFPDESLEQCKGSDSVLLSCIGGYKWDKNPRELRPETGLLRMRKEMGLFANLRPAKVIPQLMGASTLKEEVLEGVDIMVVRELTGDVYFGTPKGTETRDGKRVAYNNMIYDEEEIRRIAKVAFDVAMKRGKNVYSIDKANVLDVSQLWRDVVIEE-SKNYPEVELNHMYIDNAAMQLIRWPKQFDTIVCGNLFGDIISDEASMLVGSLGMLPSASIGDPSGPGVFEPCHGSAPDIAGEDKANPLAMILSAAMMLRYDLSRAEEAQMLEDAVEQVLDI-CRTVDIA--GGGLPTVGCKQMGEEVAKLVAALVEKKLVA 1212
            M Y  + + A+L  ++AF   P    R    +  MAV K DSY ITLL GDGIGPEIT AT   L  L ++ GF M  KEALIGG A+D  N PFP+E+L+QC  SDSVLL+CIGGYKWD NPRELRPETGLL+MRKEMGLFANLRPAKVIPQL+ ASTLK EV+EGVD+MVVRELTGDVYFGTPKG E RDG+RV +NNMIY E EI RIA+VA DVA KRG  + S+DKANVLDVSQLWR+V  +  +K +P+VEL+HMY+DNAAMQLIRWPKQFDT+V GN+FGDI+SDEASMLVGSLGMLPSASIG+ SGPGVFEP HGSAPDIAGEDKANPLAMILSAAMML YDL R EEA +LE AVE  LD   RT DI   G G   VGCK+MG  VA LV+ +   + VA
Sbjct:    5 MSYRCMAVAALLGASSAFTPAPTAFQRTQRTSLNMAVPKSDSYSITLLPGDGIGPEITTATKEVLGALCKRCGFTMEMKEALIGGCAIDEKNDPFPEETLKQCLESDSVLLACIGGYKWDSNPRELRPETGLLKMRKEMGLFANLRPAKVIPQLLDASTLKREVVEGVDVMVVRELTGDVYFGTPKGIEERDGQRVGFNNMIYGENEIVRIARVAGDVASKRGGKLCSVDKANVLDVSQLWREVTTDTITKEFPDVELSHMYVDNAAMQLIRWPKQFDTMVTGNIFGDILSDEASMLVGSLGMLPSASIGE-SGPGVFEPIHGSAPDIAGEDKANPLAMILSAAMMLTYDLERPEEAALLEAAVEAALDQGLRTADIKQEGDGCKLVGCKEMGSVVADLVSKIDLPEAVA 413