mRNA_H-akashiwo_Contig1218.1.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig1218.1.1
Unique NamemRNA_H-akashiwo_Contig1218.1.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
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BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: A0A7S3XKN4_HETAK (Acetyl-coenzyme A synthetase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XKN4_HETAK)

HSP 1 Score: 135 bits (341), Expect = 8.750e-36
Identity = 60/61 (98.36%), Postives = 61/61 (100.00%), Query Frame = 1 
Query:    7 MTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            MT+TEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV
Sbjct:    1 MTNTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 61
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BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: A0A3N2DFS5_9GAMM (Acetyl-coenzyme A synthetase n=1 Tax=Sinobacterium caligoides TaxID=933926 RepID=A0A3N2DFS5_9GAMM)

HSP 1 Score: 88.6 bits (218), Expect = 6.260e-19
Identity = 35/62 (56.45%), Postives = 46/62 (74.19%), Query Frame = 1 
Query:    4 SMTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            S+ + E+FWAEQA KFL W QP+ KV +  F  G +AWFVDGK+N +  C+D+HLPERAN +
Sbjct:   31 SIDTPEQFWAEQAEKFLTWKQPWSKVVEEDFNQGHVAWFVDGKINVSENCIDRHLPERANDI 92
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BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: A0A7S2R786_9STRA (Acetyl-coenzyme A synthetase n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2R786_9STRA)

HSP 1 Score: 85.1 bits (209), Expect = 1.040e-17
Identity = 34/62 (54.84%), Postives = 48/62 (77.42%), Query Frame = 1 
Query:    4 SMTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            S++  + FWAE+A +FL+W  PF  V QGSF DGDIAWF++GK N ++ C+DQHL ++A+QV
Sbjct:   43 SVSDPDGFWAEKAKEFLVWFSPFTSVKQGSFADGDIAWFLNGKTNVSSTCIDQHLQDKADQV 104
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BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: A0A7S2C0C2_9STRA (Hypothetical protein (Fragment) n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2C0C2_9STRA)

HSP 1 Score: 82.4 bits (202), Expect = 8.700e-17
Identity = 34/62 (54.84%), Postives = 45/62 (72.58%), Query Frame = 1 
Query:    4 SMTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            S+ + +EFW  QA  +L W + F  V QG F+ GDIAWF++GKLNA+ CC+DQHL ER +QV
Sbjct:   63 SIANPDEFWGAQARAYLSWFRDFDVVQQGDFEHGDIAWFLNGKLNASYCCIDQHLAERGDQV 124
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BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: UPI001F020A56 (acetate--CoA ligase n=1 Tax=Sinobacterium norvegicum TaxID=1641715 RepID=UPI001F020A56)

HSP 1 Score: 82.0 bits (201), Expect = 1.250e-16
Identity = 33/62 (53.23%), Postives = 43/62 (69.35%), Query Frame = 1 
Query:    4 SMTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            S+   E+FWAEQANKFL W +P+ KV +  F  G +AWFVDGK+N    C+D+HL +RAN  
Sbjct:   31 SIEQPEQFWAEQANKFLDWRKPWTKVVEQDFHQGQVAWFVDGKINVTENCIDRHLADRANDT 92
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BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: A0A2A5CB61_9GAMM (Acetyl-coenzyme A synthetase n=1 Tax=SAR86 cluster bacterium TaxID=2030880 RepID=A0A2A5CB61_9GAMM)

HSP 1 Score: 81.6 bits (200), Expect = 1.710e-16
Identity = 35/62 (56.45%), Postives = 43/62 (69.35%), Query Frame = 1 
Query:    4 SMTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            S+   E FWA QAN FL W QPF KV QG  ++G   WF  GKLN +A C+D+HLPERA+Q+
Sbjct:   31 SIDDPETFWANQANLFLDWVQPFSKVYQGDMKEGKFQWFNGGKLNVSANCIDRHLPERADQI 92
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BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: A0A7S1V5K2_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Sexangularia sp. CB-2014 TaxID=1486929 RepID=A0A7S1V5K2_9EUKA)

HSP 1 Score: 80.1 bits (196), Expect = 5.530e-16
Identity = 34/62 (54.84%), Postives = 43/62 (69.35%), Query Frame = 1 
Query:    4 SMTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            S T+  +FWA  A  FL +  PF  V QG+F DGDIAWF +GKLN +  C+D+HLP+RANQ 
Sbjct:   35 STTNPTQFWASAARSFLSFFSPFTHVSQGTFADGDIAWFTNGKLNVSYNCIDRHLPKRANQT 96
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BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: A0A6C0TPG0_9PSED (Acetyl-coenzyme A synthetase n=4 Tax=Pseudomonas TaxID=286 RepID=A0A6C0TPG0_9PSED)

HSP 1 Score: 79.3 bits (194), Expect = 1.110e-15
Identity = 32/62 (51.61%), Postives = 44/62 (70.97%), Query Frame = 1 
Query:    4 SMTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            S+   +EFWAEQA +FL W QP+ KV +     G+ AWF+DGKLN ++ C+D+HL  RA+QV
Sbjct:   31 SIEQPDEFWAEQAKRFLDWDQPWSKVHESDMSQGEAAWFIDGKLNVSSNCIDRHLATRADQV 92
[Close]
BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: A0A4P9VUB0_9GAMM (Acetyl-coenzyme A synthetase n=4 Tax=Zooshikella TaxID=202771 RepID=A0A4P9VUB0_9GAMM)

HSP 1 Score: 79.0 bits (193), Expect = 1.520e-15
Identity = 33/62 (53.23%), Postives = 41/62 (66.13%), Query Frame = 1 
Query:    4 SMTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            S+ + + FWAEQA   L W  P+ KV QG F  GD+AWFV GKLN    C+D+HLP +ANQ 
Sbjct:   30 SINNPDAFWAEQAKVHLHWFSPWEKVYQGDFAQGDVAWFVGGKLNVTYNCIDRHLPTKANQT 91
[Close]
BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Match: A0A7R9U0Y9_9STRA (Acetyl-coenzyme A synthetase n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U0Y9_9STRA)

HSP 1 Score: 78.6 bits (192), Expect = 2.070e-15
Identity = 31/62 (50.00%), Postives = 41/62 (66.13%), Query Frame = 1 
Query:    4 SMTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCLDQHLPERANQV 189
            S+   + FW   AN+ L W QPF  V  G F+ GD+AWF++GKLNA+ CCLDQHL +R  + 
Sbjct:   11 SLEDPDAFWGHHANELLAWFQPFDIVHSGGFEHGDVAWFLNGKLNASYCCLDQHLEQRGKRA 72
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig1218.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3XKN4_HETAK8.750e-3698.36Acetyl-coenzyme A synthetase n=1 Tax=Heterosigma a... [more]
A0A3N2DFS5_9GAMM6.260e-1956.45Acetyl-coenzyme A synthetase n=1 Tax=Sinobacterium... [more]
A0A7S2R786_9STRA1.040e-1754.84Acetyl-coenzyme A synthetase n=1 Tax=Rhizochromuli... [more]
A0A7S2C0C2_9STRA8.700e-1754.84Hypothetical protein (Fragment) n=1 Tax=Dictyocha ... [more]
UPI001F020A561.250e-1653.23acetate--CoA ligase n=1 Tax=Sinobacterium norvegic... [more]
A0A2A5CB61_9GAMM1.710e-1656.45Acetyl-coenzyme A synthetase n=1 Tax=SAR86 cluster... [more]
A0A7S1V5K2_9EUKA5.530e-1654.84Hypothetical protein (Fragment) n=1 Tax=Sexangular... [more]
A0A6C0TPG0_9PSED1.110e-1551.61Acetyl-coenzyme A synthetase n=4 Tax=Pseudomonas T... [more]
A0A4P9VUB0_9GAMM1.520e-1553.23Acetyl-coenzyme A synthetase n=4 Tax=Zooshikella T... [more]
A0A7R9U0Y9_9STRA2.070e-1550.00Acetyl-coenzyme A synthetase n=1 Tax=Pinguiococcus... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig1218contigH-akashiwo_Contig1218:8354..8926 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog1209072.ALBT01000044_gene2534
Preferred nameacsA
PFAMsACAS_N,AMP-binding,AMP-binding_C
Model size207
Max annot lvl1236|Gammaproteobacteria
KEGG rclassRC00004,RC00012,RC00043,RC00070,RC02746,RC02816
KEGG koko:K01895
KEGG ReactionR00235,R00236,R00316,R00926,R01354
KEGG Pathwayko00010,ko00620,ko00640,ko00680,ko00720,ko01100,ko01110,ko01120,ko01130,ko01200,map00010,map00620,map00640,map00680,map00720,map01100,map01110,map01120,map01130,map01200
KEGG ModuleM00357
Hectar predicted targeting categoryother localisation
Exons3
Evalue6.58e-17
EggNOG OGsCOG0365@1|root,COG0365@2|Bacteria,1MUF5@1224|Proteobacteria,1RMNZ@1236|Gammaproteobacteria,1FH2P@10|Cellvibrio
EC6.2.1.1
DescriptionCatalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
Cds size201
COG categoryH
BRITEko00000,ko00001,ko00002,ko01000,ko01004
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387124.1107914-UTR-H-akashiwo_Contig1218:8353..83591681387124.1107914-UTR-H-akashiwo_Contig1218:8353..8359Heterosigma akashiwo CCMP452UTRH-akashiwo_Contig1218 8354..8359 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387124.12407-CDS-H-akashiwo_Contig1218:8359..84101681387124.12407-CDS-H-akashiwo_Contig1218:8359..8410Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1218 8360..8410 +
1681387124.134737-CDS-H-akashiwo_Contig1218:8545..85901681387124.134737-CDS-H-akashiwo_Contig1218:8545..8590Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1218 8546..8590 +
1681387124.1438494-CDS-H-akashiwo_Contig1218:8821..89261681387124.1438494-CDS-H-akashiwo_Contig1218:8821..8926Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1218 8822..8926 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig1218.1.1prot_H-akashiwo_Contig1218.1.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig1218 8360..8926 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig1218.1.1

>prot_H-akashiwo_Contig1218.1.1 ID=prot_H-akashiwo_Contig1218.1.1|Name=mRNA_H-akashiwo_Contig1218.1.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=67bp
MTSTEEFWAEQANKFLLWSQPFRKVCQGSFQDGDIAWFVDGKLNAAACCL
DQHLPERANQVNFGSE*
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mRNA from alignment at H-akashiwo_Contig1218:8354..8926+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig1218.1.1 ID=mRNA_H-akashiwo_Contig1218.1.1|Name=mRNA_H-akashiwo_Contig1218.1.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=573bp|location=Sequence derived from alignment at H-akashiwo_Contig1218:8354..8926+ (Heterosigma akashiwo CCMP452)
TTTAGCATGACCAGTACTGAAGAATTTTGGGCTGAGCAGGCCAATAAGTT
TCTTTTGGTAGGTGAAACAGGTTGTGTTCAGCATTCTCTACTGTTATCTC
CTTGAATGCAGTCCTGATAATGAAAATGAGTTTTATTTGTTGCAGCCCAA
TGCCGACGCCTTATGAATTATCACCGATCTTATTCCTTGCAGTGGTCCCA
GCCATTCCGTAAAGTTTGCCAAGGCTCGTTTCAGGATGTGAGTGGATAGT
GCTGTACTGCAGCAGCAATATACAACTATTGCACAGACATTAAAAAACAT
TTGAACTGGATTTATCACCGAGCACATCGCATTTTTACAAATCAACAATG
TTTAAACGATACTATGGTGAAATAGAAGGGCCACACGGGTTTTTGGCTTA
ATAAACATTCATACAATTCTCCAGAACTTGTTTTCAATATTCCTGTTTGT
GAATGACTCTCACAACAGGGCGACATCGCGTGGTTTGTTGATGGAAAGCT
CAACGCTGCAGCCTGCTGCTTGGATCAGCACCTGCCTGAACGTGCCAACC
AGGTAAATTTCGGCAGTGAATGA
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Coding sequence (CDS) from alignment at H-akashiwo_Contig1218:8354..8926+

>mRNA_H-akashiwo_Contig1218.1.1 ID=mRNA_H-akashiwo_Contig1218.1.1|Name=mRNA_H-akashiwo_Contig1218.1.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=201bp|location=Sequence derived from alignment at H-akashiwo_Contig1218:8354..8926+ (Heterosigma akashiwo CCMP452)
ATGACCAGTACTGAAGAATTTTGGGCTGAGCAGGCCAATAAGTTTCTTTT
GTGGTCCCAGCCATTCCGTAAAGTTTGCCAAGGCTCGTTTCAGGATGGCG
ACATCGCGTGGTTTGTTGATGGAAAGCTCAACGCTGCAGCCTGCTGCTTG
GATCAGCACCTGCCTGAACGTGCCAACCAGGTAAATTTCGGCAGTGAATG
A
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