mRNA_H-akashiwo_Contig120.19.1 (mRNA) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: A0A6V1Q1T2_HETAK ((R)-citramalate synthase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1Q1T2_HETAK)

HSP 1 Score: 1268 bits (3282), Expect = 0.000e+0
Identity = 649/649 (100.00%), Postives = 649/649 (100.00%), Query Frame = 3

Query:   69 MCRFQKTFIVGAWAILVLALFCSGFVPSPHQTRQTRTLIKSISSLRMSTMDNSKEVTSEMDDVLSSQRTSNMPDGLSAKHLDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCVTDVECAAWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKMEVSDGAQGEGRRTLLEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFLALKCDAQSLECRLD 2015
            MCRFQKTFIVGAWAILVLALFCSGFVPSPHQTRQTRTLIKSISSLRMSTMDNSKEVTSEMDDVLSSQRTSNMPDGLSAKHLDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCVTDVECAAWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKMEVSDGAQGEGRRTLLEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFLALKCDAQSLECRLD
Sbjct:    1 MCRFQKTFIVGAWAILVLALFCSGFVPSPHQTRQTRTLIKSISSLRMSTMDNSKEVTSEMDDVLSSQRTSNMPDGLSAKHLDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCVTDVECAAWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKMEVSDGAQGEGRRTLLEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFLALKCDAQSLECRLD 649          

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: A0A7S2UWY3_9STRA ((R)-citramalate synthase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UWY3_9STRA)

HSP 1 Score: 656 bits (1693), Expect = 4.940e-225
Identity = 347/572 (60.66%), Postives = 420/572 (73.43%), Query Frame = 3

Query:  303 KHLDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCVTDVECAAWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKMEVSDGAQGEGRRTLLEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQ-GAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFLALKCDAQSLECRLD 2015
            + ++IYDTTLRDGTQ ES+SV+V+GKL IA+RL EFG+ YIEGGWPGSNPKD EFF RA+ EL      ++ AFGSTRRKF    +D QIA L++S   C+C+VGKS D H+D+ILEVPREENLAMV DS+ +LK  G+EVMLDLEHF DG  S+P  ++ VC+AAVDAGVDVLV+CDTNGG LPWE+    R V   FP VRLGIHCHNDQELAVAN+L A+R G ++VQGT+NG+GERTGNANL S++P LQLKMGY GVG  KL++LT +SRF DE+MNR P  + P+VGASAFAHKGGIHVSA+AKNP SYQH++P+ VGN +RVLVS+LAGRSNI+ KV+E+GL   D +   W + S+QILN +K LEK GY+FEGAEASV LMLRR  + Y PPF I DY  QI DQ   S     R +  +        G ARATVK+ V +    E  + LLEVAEGNGPV+AL  AL +GL P FP+L E+ LSDYKVRILD   G   T+S  RVM++FK+ N      + +TTVG DSNIISAS NAL DG EY L  +     L C +D
Sbjct:   90 QEVEIYDTTLRDGTQAESISVSVEGKLRIAERLAEFGVHYIEGGWPGSNPKDKEFFERAKTELSPEARTKLTAFGSTRRKFKKAAEDSQIAALVDSGVDCICLVGKSSDFHVDKILEVPREENLAMVHDSIHYLKGLGKEVMLDLEHFIDGFKSNPQYSMSVCQAAVDAGVDVLVMCDTNGGSLPWEVAHVTREVRKMFPEVRLGIHCHNDQELAVANTLEAVREGATVVQGTINGLGERTGNANLCSVIPNLQLKMGYHGVGD-KLKDLTSVSRFSDELMNRSPNPAAPFVGASAFAHKGGIHVSAIAKNPLSYQHIEPEEVGNLKRVLVSELAGRSNIMTKVEEYGLIGQDSDLNVWKERSVQILNKVKALEKSGYTFEGAEASVNLMLRRSSDMYTPPFAIQDYMLQIWDQDVDS---VMRFSDPSVVEPYYFHGSARATVKVLVMEHTCNE--KQLLEVAEGNGPVNALANALMKGLVPTFPVLEELELSDYKVRILDMSCGKSGTSSVVRVMMDFKKSNG-----DKYTTVGADSNIISASLNALVDGLEYALIGE-----LNCAVD 645          

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: D8LTV2_ECTSI ((R)-citramalate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LTV2_ECTSI)

HSP 1 Score: 610 bits (1573), Expect = 1.090e-207
Identity = 329/572 (57.52%), Postives = 398/572 (69.58%), Query Frame = 3

Query:  285 PDGLSAKHLDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCV-TDVECA-AWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKMEVSD-------GAQGEGRRTLLEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFL 1973
            P+G   + +++YDTTLRDGTQ E +S +V  KL IA +L  FGM YIEGGWPGSNPKD EFF RAR ELP   W RVVAFGSTRRKF    +DKQ+  L+ +  GCV IV K+WDLH+D ILEVPREENLAMV +SV HLK AG+EVMLDLEHFFDG  ++   T+ VC AAV A VDVLVLCDTNGG LPWEI+  VR V A +PG RLGIHCHNDQ LAVANSL A+R+G  +VQGTVNG+GERTGNANL +++PTLQLKMG  GVG   L  LT LSRF DE  N    +  P+ G SAFAHKGGIHVSA+ K P SYQH+DP  VGN +RVLVS+L+G+SNI++KV++ G+   T+++ +  W +   Q+L  +K LE  GYS+EGA+ASV L++RR +  Y PPF ++DY  QI D   T   +        A          RATVK+ V         G +GE     LEVAEGNGPVDAL + L R L P FP L+ V+LSDYKVRILD + A  T + TRV+IEF   +   G    W+TVGV++NIISAS +AL DGFEY L
Sbjct:   37 PEGEVLRTVEMYDTTLRDGTQMEGISASVLDKLKIALQLHHFGMHYIEGGWPGSNPKDVEFFERARKELPAEAWERVVAFGSTRRKFKACAEDKQLESLISAGTGCVTIVAKAWDLHVDHILEVPREENLAMVRESVEHLKMAGKEVMLDLEHFFDGWKANREYTMQVCEAAVGAKVDVLVLCDTNGGSLPWEIDEIVRDVKAAYPGTRLGIHCHNDQGLAVANSLQAVRSGADVVQGTVNGIGERTGNANLMTIIPTLQLKMGMTGVGE-HLTNLTALSRFTDEQFNMAHTSGAPFTGISAFAHKGGIHVSAVNKIPDSYQHIDPAQVGNMKRVLVSELSGQSNILSKVEDSGMASKTELKASKVWRERMGQVLKTVKRLETEGYSYEGADASVNLLVRRTMSGYVPPFKLLDYLVQIWDSDRTPGGLEFPSGPTTA----------RATVKVRVPSMDSEAELGIEGENPGVRLEVAEGNGPVDALAKGLFRALLPTFPSLQSVKLSDYKVRILDQESA--TQAKTRVLIEFYEKSSDVG----WSTVGVNTNIISASMSALMDGFEYAL 591          

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: A0A836CCG4_9STRA ((R)-citramalate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CCG4_9STRA)

HSP 1 Score: 568 bits (1464), Expect = 3.960e-190
Identity = 333/648 (51.39%), Postives = 403/648 (62.19%), Query Frame = 3

Query:  309 LDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVV-----------AFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVG--------------ERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGL-CVTDVECAAWADSSLQILNDIKVLEKMGYSFEGAEASVEL------------------MLRRMVENYAPPFNIVDYSCQIMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKMEVSDGAQ---------GEGRRTL-LEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYK---------------------------VRILDHQGAKATASTTRVMIEF--KRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFLALKCDAQSLE 2003
            +DIYDTTLRDGTQ E +SV+VD KL IA+ L  FGM YIEGGWPGSNPKDAEFF RA+AE P   W R+            AFGSTRRK    EQDKQ+A L+ +   CV IV K+WDLH+D ILEVPREENLAMV +SVA LKAAG+EVM+DLEHFFDG  ++PA T+ VC  AV+AG DVLVLCDTNGG L WE++  VR VA+ FPGVRLGIHCHNDQELAV+NSL A+++G ++                       ERTGNANLAS+VPTLQLKMG AGVG  +L ELT L RFVDE +NR+P +  P+VG SAFAHKGGIHVSA+ K P SYQH+DP  VGN RR LVS+L+GRSNI++KV+EFG+    +     W D   ++L  +K LEK G +FEGA+A+V L                  M+RR++  Y  PF ++DY+    D + +      RG        ++ S   RATVK+++  G             G RT  LE AEGNGPV+AL +AL + L P FP L+ V L DYK                           VRILD + A  TA+ TRVMIEF    HN+       W TVGVD+NIISAS  AL DGFEY L       SL+
Sbjct:   34 VDIYDTTLRDGTQMEGISVSVDDKLKIARVLSNFGMHYIEGGWPGSNPKDAEFFERAKAEPPPDVWSRIAEXXXXXXXXXAAFGSTRRKNSTCEQDKQLATLIAAGTNCVTIVAKAWDLHVDHILEVPREENLAMVGESVAFLKAAGKEVMIDLEHFFDGFKANPAYTMQVCERAVEAGADVLVLCDTNGGTLGWEVDQAVRTVASAFPGVRLGIHCHNDQELAVSNSLQAVQSGATVXXXXXXXXXXXXXXXXXXXXXXXERTGNANLASIVPTLQLKMGCAGVGE-RLPELTALCRFVDEQLNRLPDSKAPYVGQSAFAHKGGIHVSAVNKIPDSYQHIDPAAVGNVRRTLVSELSGRSNILSKVEEFGMGSKEEFSSPEWQDRMNRVLTAVKTLEKRGSTFEGADATVNLIEPLRXXXXXXXXXXXXXMIRRLMPGYTAPFRLMDYTVTAADTMGSD---ALRGEETGTPGSVMPS--VRATVKVKLPAGVSQMESLASFWDNGDRTQRLESAEGNGPVNALAKALNKALLPTFPSLQHVVLKDYKASRRPAQLPELRVFVGEVVQELKFQLKVRILDQESA--TAANTRVMIEFMDSHHNQ------QWATVGVDTNIISASMCALMDGFEYALVEYAPCCSLD 667          

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: A0A1Z5JTT2_FISSO ((R)-citramalate synthase n=1 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JTT2_FISSO)

HSP 1 Score: 555 bits (1429), Expect = 1.100e-185
Identity = 300/583 (51.46%), Postives = 388/583 (66.55%), Query Frame = 3

Query:  303 KHLDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPG-VRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCVTDVECAAWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQVATSHT--VTQRGAAAAAAAGLLASGQARATVKMEVSDGAQGEGR---------RTLLEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFLALKCDAQSLECRLD 2015
            KH  IYDTTLRDGTQGESVS + D KL I +RL EFG+ YIE GWPGSNPKDAEFF RA+ ELP +   ++VAFGSTRRK    +QD Q+ +L++S+A  VCIV KS    + EIL+   +ENL M+ D+V HL  AGR+VM+D+EHFFDG A+DPA  L  C AA DAG   LVLCDTNGG +PW +    +RV   F   V +GIHCHND  +AVANS++A + G  L+QGT+NG+GERTGNA+L S++PTL L MG     + +L  LT +SR+VDE++NR P  + P+VG SAFAHKGG+HV+AM ++P SYQH+DP +VGN  RVL+S+L+GR NI++K++  G+   D+      D +L ILN +K LE +GY+FEGAEASV LM+      Y PPF ++DYS Q+ D    S T  + + G ++++ A       ARAT+K+    G+  +              LEV +GNGPVDAL  AL R + P+ P L+ + L DYKVRILD Q A  T + TRVMIEF+   + T     WTTV VD+N+ISAS NAL DGFEY L     +  L   LD
Sbjct:   60 KHAIIYDTTLRDGTQGESVSASADDKLKICRRLAEFGVDYIEAGWPGSNPKDAEFFRRAQTELPETARAKLVAFGSTRRKNTSADQDTQVQLLIDSKAPTVCIVAKSHSWQVQEILKADLQENLDMIRDTVQHLTNAGRQVMVDMEHFFDGYAADPAYALQCCEAAADAGATCLVLCDTNGGTMPWTVGQVSQRVVRHFDRYVTIGIHCHNDCGMAVANSVSACQNGIGLIQGTINGIGERTGNADLCSIIPTLALHMGSNIACKKQLLNLTSVSRYVDEILNRSPNVAAPYVGISAFAHKGGLHVAAMERSPLSYQHIDPALVGNEMRVLISELSGRQNILSKMEAAGV---DISETGTNDRALAILNRVKELEAVGYTFEGAEASVHLMILHATRGYCPPFRVLDYSAQVYDSNIDSATRVMKKHGISSSSLAPT-----ARATIKVRTVSGSSDDDDCDTTNRPPFEDRLEVGDGNGPVDALAAALTRAVTPSHPYLKNIELVDYKVRILDPQSA--TQAATRVMIEFRDTQKDT----KWTTVSVDTNVISASLNALVDGFEYALIEHSQSCPLYDDLD 628          

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: A0A7S4QUS5_9STRA ((R)-citramalate synthase n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4QUS5_9STRA)

HSP 1 Score: 541 bits (1394), Expect = 6.290e-180
Identity = 310/642 (48.29%), Postives = 417/642 (64.95%), Query Frame = 3

Query:  135 SGFVPSPHQTRQTRTL-IKSISSLRMST------MDNSKEVTSEMDDVLSSQRTSN-----MPDG--LSAKHLDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCVTDVECAAWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQ-VATSHTVTQRGAAAAAAAGLLASGQARATVK---MEVSDGAQGEGRRTL-----LEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFLALKCDA 1991
            + FV   H++++TR   ++S S+ +++T       +N K ++  ++D L  + +S+     + DG  +    + +YDT+LRDGTQGES+S + + KL IA RL  FG+ YIE GWPGSNPKDAEFF RA+ EL  S   ++VAFGSTRRK + VEQD QI MLL+S A   CIV KS    + +I+    +ENL M+ADSVA+L +  R V +DLEHFFDG   D    +  C+AAVDAG   LVLCDTNGG +PW++     +V   FP V +G+H HND  +AVANSL A ++G  ++QGT+NG+GERTGNA+L S+VP+L L +         L  +T LSRF+DE +NR P ++ P+VGASAFAHKGG+HV+AM ++P SYQHV+P +VGN +RVLVS+L+GR NI+ K ++ GL V   +     + +  ILN +K LE +GY+FEGA+ASV LM+      Y PPF I+DYS Q+ D  + ++  V ++G    A    + S  ARAT+K   + + D A   G   L     LEV++GNGPVDAL  AL+R L P  P L  + L+DYKVRILD +  KAT +TTRVMI+F    E T   ESWTTV VD N+ISAS NAL DGFEY L    D+
Sbjct:   23 TAFVVPNHKSKRTRAFHVESSSTTQVTTNKNNVDAENDKWLSESIEDELQKKSSSSSSGHAVDDGAVVGPGAVLVYDTSLRDGTQGESISASCEDKLKIANRLSTFGVDYIEAGWPGSNPKDAEFFTRAQTELSPSAKSKLVAFGSTRRKAIAVEQDAQIQMLLDSHAPTCCIVAKSHLWQVTDIIRATPQENLDMIADSVAYLTSQNRRVFIDLEHFFDGYTFDEDYAMKCCQAAVDAGAKGLVLCDTNGGSMPWDVAHITSKVVQTFPQVTVGMHAHNDCGMAVANSLMAAKSGAGMIQGTINGIGERTGNADLCSIVPSLSLHVKSNLTCNPSLANITSLSRFIDETLNRSPDSAAPYVGASAFAHKGGLHVAAMERSPMSYQHVEPALVGNEKRVLVSELSGRQNILGKFRDAGL-VDHPDDDPVEERASAILNRVKHLESIGYTFEGADASVHLMILHSTRGYCPPFRILDYSAQVYDNNMDSASRVLEKGKLEHANG--MGSSTARATIKVRTLNLDDEAYTSGDAPLAFIDRLEVSDGNGPVDALSSALKRALSPPHPFLNTIELTDYKVRILDPE--KATGATTRVMIDF----EDTTTGESWTTVSVDRNVISASLNALVDGFEYALVQHSDS 655          

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: R7QGZ5_CHOCR ((R)-citramalate synthase n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QGZ5_CHOCR)

HSP 1 Score: 535 bits (1378), Expect = 5.220e-179
Identity = 290/558 (51.97%), Postives = 373/558 (66.85%), Query Frame = 3

Query:  309 LDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCVTDVECAA---WADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKMEV---SDGAQGEGRRTLLEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFE 1964
            + +YDTTLRDG QGE +S++V  KL IA RL EFG  YIEGGWPGSNPKDA FF   R +L  +   ++VAFGSTR K    E+D+ + ML  ++   V +VGK+WD+ +  +LE   EENL M+ +SVA+ K  GREVMLD EHF+DG  + P   +   +AAVD GVDVLVLCDTNGG +PW+++     V   FP  R+G+HCHND ELAVANS++A+ AG SLVQGTVNG GERTGNANL S+VPTLQLK+GYA VG A L +LT LSRFVDE+  + P + QP+VG S+FAHKGG+HV+A+ KN  +YQH+DP +VGN +R+LVS+LAGR N+++K +E GL     +  A   W + + Q+L  +K LE  GYSFEGAEAS+ELMLRR VE Y PPF ++D+S    D     +     G               +ATVK+ +    DG +    +  LEVAEGNGP+DA+  AL + L   +  L+ V L+DYKVRILD + A  TA+TTRV+++F    E +     WTTV    NII AS NAL DGFE
Sbjct:    8 IQVYDTTLRDGAQGEGISLSVADKLKIATRLDEFGAHYIEGGWPGSNPKDAAFFENCRGKLKRA---KLVAFGSTRYKNTTCEKDRNVQMLANADTLVVTLVGKAWDMQVQVVLEASLEENLDMIRESVAYFKRLGREVMLDAEHFYDGYKAAPEYAIACLQAAVDGGVDVLVLCDTNGGSVPWDLQKVTATVVHRFPATRVGVHCHNDMELAVANSISAVNAGASLVQGTVNGYGERTGNANLMSIVPTLQLKLGYACVGDA-LSKLTSLSRFVDEIACQKPVSGQPFVGVSSFAHKGGLHVAAVLKNEDTYQHIDPSLVGNEKRILVSELAGRRNVLSKAEELGLVPAGDDAGARQEWGNRAKQVLGQVKDLENKGYSFEGAEASLELMLRRSVEGYRPPFELLDFSVTT-DNKRIHYGPNSEGPP-------FNESVTQATVKIALLGPKDGGEACPTKICLEVAEGNGPIDAVNGALGKVLFSMYQPLQTVALTDYKVRILDSESA--TAATTRVVVDF----EDSASGRQWTTVYAHPNIIVASVNALMDGFE 547          

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: A0A2V3IHB1_9FLOR ((R)-citramalate synthase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3IHB1_9FLOR)

HSP 1 Score: 531 bits (1367), Expect = 4.930e-177
Identity = 293/569 (51.49%), Postives = 378/569 (66.43%), Query Frame = 3

Query:  303 KHLDIYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCVTDV--ECAAWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQ----------IMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKMEV---SDGAQGEGRRTLLEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFE 1964
            K + IYDTTLRDG+QGE +S+TV  KL +A+RL EFG  YIEGGWPGSNPKD  FFA+ +  L  +   ++VAFGSTR K    E DK +  L++++   V +VGK+WD  +  +LE   +ENLAM++D+VA  K  GREVMLD EHF+DG  + PA  L    AAV AGVDVLVLCDTNGG LPWEI+   R V AEFP VR+GIHCHND ELAV+N++AA+ AG +LVQGT+NG GERTGNANL S+VPTLQLKMG+  VG  +L++LT LSR+ DE+ N+    ++P+VGAS+FAHKGG+HV+A+ K+  +YQH+DP +VGN RR+LVS+L+GR NI+ K +E GL   D   +   W      +L  +K LE  GYSFEGAEAS+ELMLRR V  Y PPF ++D+S            +++    + +VTQ                  A+VK+ +    DG      +   E+AEGNGPVDA+  AL++ L P +  LR V L DYKVRILD+  A  TA+ TRVM+EF+  +      + WTTV    NII AS NAL DGFE
Sbjct:   29 KTVQIYDTTLRDGSQGEGISLTVPDKLKLAQRLDEFGAHYIEGGWPGSNPKDESFFAQCKG-LDNA---KLVAFGSTRYKGTTCEHDKNVQALMKADTPVVTLVGKAWDHQVSVVLEATLDENLAMISDTVAFFKRKGREVMLDAEHFYDGYKASPAYALSCLHAAVKAGVDVLVLCDTNGGTLPWEIDEITRVVVAEFPTVRIGIHCHNDMELAVSNTIAAVHAGATLVQGTINGFGERTGNANLMSIVPTLQLKMGHDCVGD-RLQQLTDLSRYTDEITNQPHVPNRPYVGASSFAHKGGLHVAAVLKDENTYQHIDPVLVGNERRILVSELSGRRNILTKAEELGLHPADESKDGVEWNVRVKAVLEQVKELENKGYSFEGAEASLELMLRRTVHGYRPPFELLDFSVSTDNKRVVYGNVLNATPNNESVTQ------------------ASVKLALLGPEDGGDQCPTKICFEIAEGNGPVDAVNGALQKCLLPVYAPLRSVSLVDYKVRILDN--ASGTAAITRVMVEFEESSNG----KRWTTVYAHPNIIVASVNALMDGFE 568          

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: A0A7C5E488_9CHLR ((R)-citramalate synthase n=1 Tax=Anaerolineae bacterium TaxID=2052143 RepID=A0A7C5E488_9CHLR)

HSP 1 Score: 518 bits (1333), Expect = 8.630e-173
Identity = 295/553 (53.35%), Postives = 362/553 (65.46%), Query Frame = 3

Query:  315 IYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEFPGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKEFGLCVTDVECAAWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKMEVSDGAQGEGRRTLLEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFL 1973
            +YDTTLRDGTQGE +S++ + KL IA++L EFG  YIEGGWPGSNPKDAEFFAR + EL L    ++ AFGSTR K    ++D  I  L+E+    V +VGKSWDLH+  ILE   EENL M+A+SVA+ K  G+EV+ D EHFFDG  ++P   L   +AA+ +G D +VLCDTNGG  PWE+E   ++VAA F G RLGIH HND EL VAN+LA +R G   VQGTVNG GER GNANL S++P LQLKMGY  V   +L  LT LSR+VDE+ N  P   QP+VG SAFAHKGGIHV+A+ K   SYQH+DP VVGN +R +VS+L+GR NI+ K  E GL  +  E         Q+L  IK LE  GY+FEGAEASV++MLRR    Y PPF ++D+            T  ++G       GL       ATVK++V D    E R T   VAEGNGPV+AL  ALRR L   FP L+ V L+D+KVRILD      TA+TTRVMI+F++     G TE WTTVG   NII AS+ AL D  EY L
Sbjct:    6 LYDTTLRDGTQGEGISLSCNDKLKIAQKLDEFGAHYIEGGWPGSNPKDAEFFAR-QGELKLKH-AKIAAFGSTRYKNTTCDKDANIQALVEANTPVVTLVGKSWDLHVRHILETDLEENLEMIAESVAYFKERGKEVIYDAEHFFDGYKANPDYALLTLQAAIKSGADCVVLCDTNGGSTPWEVEEITKQVAARFEGQRLGIHPHNDCELGVANALAGVRGGARHVQGTVNGYGERVGNANLISIIPDLQLKMGYECVKPEQLAGLTALSRYVDELANMTPNPHQPFVGYSAFAHKGGIHVAAILKVEESYQHMDPTVVGNEKRAVVSELSGRGNILYKAAERGLDTSREEAR-------QVLEQIKELENQGYTFEGAEASVDMMLRRANSGYKPPFEVIDFMVI---------TENRQGR------GLFT----EATVKVKVGD----EIRHT---VAEGNGPVNALNLALRRALYQDFPQLQRVHLTDFKVRILDSDAG--TAATTRVMIDFQQE----GTTEFWTTVGAHPNIIEASWRALIDSMEYAL 517          

BLAST of mRNA_H-akashiwo_Contig120.19.1 vs. uniprot
Match: A0A448Z9P0_9STRA ((R)-citramalate synthase n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448Z9P0_9STRA)

HSP 1 Score: 520 bits (1340), Expect = 2.310e-172
Identity = 287/569 (50.44%), Postives = 372/569 (65.38%), Query Frame = 3

Query:  315 IYDTTLRDGTQGESVSVTVDGKLAIAKRLGEFGMAYIEGGWPGSNPKDAEFFARARAELPLSTWRRVVAFGSTRRKFLPVEQDKQIAMLLESEAGCVCIVGKSWDLHIDEILEVPREENLAMVADSVAHLKAAGREVMLDLEHFFDGMASDPACTLDVCRAAVDAGVDVLVLCDTNGGRLPWEIEAGVRRVAAEF-PGVRLGIHCHNDQELAVANSLAALRAGCSLVQGTVNGVGERTGNANLASLVPTLQLKMGYAGVGRAKLRELTGLSRFVDEMMNRVPAASQPWVGASAFAHKGGIHVSAMAKNPASYQHVDPDVVGNTRRVLVSDLAGRSNIIAKVKE-FGLCVTDVECAAWADSSLQILNDIKVLEKMGYSFEGAEASVELMLRRMVENYAPPFNIVDYSCQIMDQVATSHTVTQRGAAAAAAAGLLASGQARATVKME-VSDGAQGEGRRTL----LEVAEGNGPVDALGQALRRGLEPAFPLLREVRLSDYKVRILDHQGAKATASTTRVMIEFKRHNEFTGLTESWTTVGVDSNIISASFNALSDGFEYFLALKCDAQSL 2000
            I+DTTLRDGTQGE VS + D KL IA RL  F + YIE GWPGSNPKD EFF RA+AEL   T  ++VAFGS+RRK   VE+D QI  L+ES+   VC+V K+    + EIL   REENL M+ DSV +L   G+ V++DLEHFFDG   DP   +  C +AVDAG   LV+CDTNGG +PWE+      +   F P   +GIHCHND  +AVANS+A+  AG  L+QGT+NG+GERTGNA+L S+VP+L L +      + K+ ELT LSRFVDE++NR P  + P+VG SAFAHKGG+HV+AM ++P SYQHV+P+ VGN  RVL+S+L+GR NI+ K+++ FG   ++++    +  SL +LN +K LE MGY FEGA+ASV LM+    + Y PPF ++DYS Q  DQ   S +   +  A         S  ARAT+K+  VSD    EG        LEV++G GPVDAL +A+ + L P+ P L  + L+DYKVRIL+   A  T + TRV+I+F+         + WTTV VDSNI+SAS NAL DGFEY L    D   L
Sbjct:   68 IFDTTLRDGTQGELVSASCDDKLKIATRLAAFDVDYIEAGWPGSNPKDLEFFKRAKAELTEHTRSKLVAFGSSRRKNTAVEKDPQIQALVESDVSTVCMVVKAHSWQVTEILRATREENLQMIYDSVEYLTNLGKTVLVDLEHFFDGYKDDPEYAIACCESAVDAGARCLVMCDTNGGSMPWEVTDTTTAMVKHFEPFCTIGIHCHNDSGMAVANSIASCHAGVGLIQGTINGIGERTGNADLCSIVPSLALHVETDMTCKDKVPELTKLSRFVDEVLNRTPNTAAPYVGRSAFAHKGGLHVAAMERSPLSYQHVEPESVGNEMRVLISELSGRQNIMGKMQDMFG---SEMDVGQKSARSLAVLNRVKELENMGYQFEGADASVHLMILYSTQGYCPPFQVLDYSAQCYDQTMDSASRLMKTRAD--------SSTARATIKVRTVSDDPDLEGSGLSFVENLEVSDGAGPVDALAKAMLKALTPSHPSLESIELTDYKVRILNPNAA--TKAVTRVLIDFRDAET----DQKWTTVSVDSNIVSASLNALVDGFEYALIEHSDTCML 619