mRNA_H-akashiwo_Contig113.11.3 (mRNA) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: A0A6H5KWQ0_9PHAE (AMP-binding domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KWQ0_9PHAE)

HSP 1 Score: 702 bits (1811), Expect = 8.760e-245
Identity = 362/665 (54.44%), Postives = 463/665 (69.62%), Query Frame = 1

Query:   67 GTVWVTEPNAYLEPKIGAKGTVAGEPARTVWQMLAETVDNFPNRPALCFKEI-GGQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKAS---RGNGAGRGGAAPRAM---IRVYSWAEFMNLGETGSNQEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTMSMDFTLMCENDRAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKMA-DNGEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEPDTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMY 2037
            GTVW TEP+A L  K+G  G  A +P  TV+++  + V+N     AL FK+  GG +A D P+  +TW+++  E ++ GKS+++LG  PH+  NIIGFNS  W +A  G++AAGG+PAGIY +N  EA +YIT HSEA VV +E+  QL K+V++ A++ S+KA    RGN        P        VY+W +FM LG    +  + ER++ Q+PG   SLIYTSGTTG PKAVMISHDN+TWT +T++  F L  E  R VS+LPLSHIAAQ+LD + PM  G  + FAQPDALKGSL  TL+E+RPT FFGVPRVWEK+YEKMQE+GRS  G KK I+TWAK KG    R+ QYGAGGG PCGFGVANA+  SKVKA LGLD       GAAP+  E+ +YFASL+I I E+FGQSE TG  T N   AWK+GT GRP+ G+E+K+  + GE C+R R++ MGYM   EKTAETID +GW+H+GDV  FD+D DDR   G +GF+ ITGRIKELIITAGGEN+PPVLIE +FK  +P I+NCM +GDKRKFL +++ L  E    D   T++LAG+SL   + IGS ATT++E   D KW  Y D  +   N++ TSNAQ+VAKW ++  DFS +GG LTPTLKLKRGP +E +A  IE +Y
Sbjct:    3 GTVWATEPHADLPIKMGTSGAAA-KPPTTVFKVFQKAVENHGENVALKFKDTSGGVSADDAPWQTLTWAQFYMETLRFGKSLMSLGFAPHRSINIIGFNSTEWFIANMGAIAAGGIPAGIYTSNLPEACQYITNHSEAEVVVVENEAQLDKFVKLAATLTSVKAIVMYRGN-------VPEGTDCGFPVYTWDQFMELGGEIPDSSIQERIEAQKPGECCSLIYTSGTTGKPKAVMISHDNMTWTAETLTTQFPLN-ETSRVVSYLPLSHIAAQILDMHGPMFLGCTVAFAQPDALKGSLVKTLREIRPTVFFGVPRVWEKMYEKMQEVGRSIKGAKKSISTWAKGKGLAHARMQQYGAGGGVPCGFGVANALAFSKVKANLGLDQATCCFTGAAPIAVEIIEYFASLNIHILELFGQSECTGPQTFNTAEAWKIGTCGRPMAGTESKLVPETGELCYRGRHIFMGYMHNPEKTAETIDEDGWMHSGDVATFDEDGDDRT-EGPSGFMQITGRIKELIITAGGENVPPVLIENEFKTELPCIANCMVVGDKRKFLSILIALRSEV-AEDGTPTSKLAGDSLTACEKIGSTATTIEEAKADPKWTEYIDKAVKACNNRTTSNAQRVAKWALLPHDFSVEGGELTPTLKLKRGPASEKYADIIEGLY 656          

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: A0A835Z6J1_9STRA (Long chain acyl-CoA synthetase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z6J1_9STRA)

HSP 1 Score: 694 bits (1790), Expect = 1.280e-241
Identity = 353/656 (53.81%), Postives = 457/656 (69.66%), Query Frame = 1

Query:   76 WVTEPNAYLEPKIGAKGTVAGEPARTVWQMLAETVDNFPNRPALCFKEIGGQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKASRGNGAGRGGAAPRAMIRVYSWAEFMNLGETGSNQEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTMSMDFTLMCENDRAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKM-ADNGEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEPDTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMYK 2040
            WVTEP+  +  ++GA+G VA  P+RT+ ++    + NF   PAL ++  G +     P++ +TW++Y  E +K  K++++LG  PH+C NIIGFNS  W +A+ G + AGG+ AGIY TN  EA +YI+ HS+A VV +E   QL KY+ +   +K LKA    G       P   + VY+W++F  LG+   + +V ERM  QQPGH   LIYTSGTTG PKAVM+SHDNITWT +T  M F+     DR++S+LPLSHIAAQ+LD Y PML G  +YFAQPDAL+GSLG TLK VRPT F GVPRVWEKIYEKMQE+G+ T G KKKIATWAK KG+E  R+ QYG GGG PCG+ VAN VV +KVK  LGLD        AAP+  E+ +YF SLD+PI+EVFGQSE TG HT+N P  W +GT GRP+ G+ENK+ AD GE C+R R++ MGYM   EKTAETID EGWLH+GDV  FDD+ +   +   +GF+ ITGRIKELIITAGGEN+ PVLIE +FK A+P++SNCM +GDKRK+L ++L+L  E + ++   T++LAG +L  SKAIGS ATTV E     KW  + +A  +  N +A S AQ + KW ++  DFSE+GG LTPTLKLKRGPTA+     I+++YK
Sbjct:    7 WVTEPDEVIPIRMGAEG-VAARPSRTMCEVFKSALANFGTHPALKYQAEGIEG----PWSTLTWTDYYNESVKFAKTLMSLGFAPHRCVNIIGFNSVEWFVAEMGCILAGGIAAGIYTTNLPEACRYISDHSQAEVVVVEGALQLKKYISIAGQLKHLKAIVVYGNETLAPVPDCPVPVYTWSDFQQLGKDVGDAQVEERMAGQQPGHCCCLIYTSGTTGTPKAVMLSHDNITWTAQTALMHFSFKI-GDRSISYLPLSHIAAQILDMYGPMLCGSCVYFAQPDALRGSLGATLKAVRPTLFLGVPRVWEKIYEKMQEVGKKTVGTKKKIATWAKQKGAEHTRMCQYGGGGGEPCGYNVANKVVFTKVKENLGLDQAKGCFSAAAPISTEILEYFGSLDLPIYEVFGQSECTGPHTINDPEGWLIGTCGRPMRGTENKIVADTGELCYRGRHIFMGYMHDPEKTAETIDEEGWLHSGDVATFDDN-NRTDMPAPSGFMRITGRIKELIITAGGENVAPVLIENEFKKALPSLSNCMVVGDKRKYLTILLSLKTELNEAEGIPTDKLAGTALETSKAIGSEATTVPEAATCPKWREHIEAARSKVNEEAPSRAQVIQKWALLPMDFSEKGGELTPTLKLKRGPTADKHRDIIDALYK 655          

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: D7G3T1_ECTSI (Long chain acyl-coA synthetase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G3T1_ECTSI)

HSP 1 Score: 672 bits (1733), Expect = 6.280e-233
Identity = 350/667 (52.47%), Postives = 452/667 (67.77%), Query Frame = 1

Query:   67 GTVWVTEPNAYLEPKIGAKGTVAGEPARTVWQMLAETVDNFPNRPALCFKEI-GGQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKAS---RGNGAGRGGAAPRAM---IRVYSWAEFMNLGETGSNQEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTMSMDFTLMCENDRAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKMADN-GEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEP--DTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMY 2037
            GTVW TE +A L  KIG  G  A  PA+TV+++  + V+N    PAL FK+  GG +A D P+  +TW++Y  E ++ GKS+++L   PH+C NIIGFNS  W +A  G++AAGG+ AGIY +N  EA +YIT HSEA VV +E+  QLAK+V++ A++ S+KA    RGN        P        VY+W +FM LG    + ++ ER++ Q+PG   SLIYTSGTTG PKAVMISHDN+TWT +T+   F      +R VS+LPLSH+A QLLD + PM    +++FAQPDALKG+L  TL+EVRPT F GVPRVWEK+YEKMQEIGRS  G K  ++ W+K +G    R  +Y  GGG P GF +ANA+V SKVK  LGLD  N     AAP+ +E  +YFASL+I I EVFGQSE TG   V+   AWK+G+ GRP+ G+E+K+    GE C+R RN+ MGYM   EKTAETID +GW+H+GDV  FD+D D R+  G +GFV ITGRIKELIITAGGENIPPVLIE +FK  +P I+NCM IGDKRKFL +++ L  E   D +    T RL G+SL   + IGS ATT++E   D KW  Y +  M   N++ TSNAQ+VAKW  +  DFS +GG LT TLKL+RGPT+E ++  IE +Y
Sbjct:    3 GTVWATERDADLPVKIGTAGAAA-RPAKTVFEVFQKAVENHGENPALMFKDTSGGMSADDAPWQTLTWAQYYMETLRFGKSLLSLDFAPHRCVNIIGFNSTEWFIANMGAIAAGGIAAGIYTSNLPEACQYITNHSEAEVVVVENEAQLAKFVKLAATLTSVKAIVMYRGN-------VPEETECGFPVYTWKQFMELGSKIPDTKIEERIEAQRPGQCCSLIYTSGTTGQPKAVMISHDNMTWTAETLCAPFN-WSHAERGVSYLPLSHVAGQLLDMHAPMAVASSVWFAQPDALKGTLVKTLREVRPTTFVGVPRVWEKMYEKMQEIGRSIHGAKAALSAWSKGRGLANARRKEYSGGGGTPWGFKIANALVFSKVKEKLGLDKANLCFTTAAPIAQETLEYFASLNIHIMEVFGQSECTGPMAVSTKEAWKMGSCGRPMLGTESKLEPGTGELCYRGRNIFMGYMHNPEKTAETIDEDGWMHSGDVATFDEDGDPRI-EGPSGFVRITGRIKELIITAGGENIPPVLIESEFKAELPCIANCMVIGDKRKFLTILIALRSEKREDENGGSSTVRLVGDSLTACEKIGSTATTIEEAKADTKWTEYINKAMKACNARTTSNAQRVAKWAFLPHDFSVEGGELTETLKLRRGPTSEKYSDIIEGLY 659          

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: A0A4D9DBU9_9STRA (AMP-binding domain-containing protein n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9DBU9_9STRA)

HSP 1 Score: 659 bits (1699), Expect = 5.990e-228
Identity = 350/639 (54.77%), Postives = 443/639 (69.33%), Query Frame = 1

Query:  148 RTVWQMLAETVDNFPNRPALCFKEI--GGQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKAS---RGNGAGRGGAAPRAMIRVYSWAEFMNLGETGSNQEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTM--SMDFTLMCENDRAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKM-ADNGEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEPDTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMYK 2040
            +TV+Q+  ETV  + + PAL +K++  GG  A    ++  TW EY +  +K  KS+++LG   H   N+IGFNS  W +A  G++AAGGV  GIY +NG +A KYIT+HSEA VV +E+ +QL KY+++   +  LKA     G   G      +  + +YSW +FM LG    ++ V  R++ Q+PGH  +LIYTSGTTGPPKAVMISHDN+TWTVK    S+ FTL CE DR+VS+LPLSH+AAQ+LD + P+  G  IYFAQPDAL+GSL +TLK+V PTYFFGVPRVWEKIYEKMQE+ RSTTG+K+ +A WAKAKG E+NR  QYG GGGAP GFG A+A+VLSKVKAALGL         AAP+  E+ +YFASLDIP+ E+FGQSE TG HT N   AWK+G++GR +PG + K  A+  E+C   R++MMGYMKME+KT E +D EGWLH+GDV + D D          GF SITGRIKELIITAGGENIPPVLIE +   A+PA++NCM +GDK+KFL ++LT+  + D      T  L  E+L   K IGS A+T ++V  D  W  YFD G+  ANS ATSNAQ V KW V+  DFSE+GG LTPTLKLKR   AE +A  I  MYK
Sbjct:   28 KTVFQVFEETVKKYGDSPALHYKKVPHGGSLAT-TEWSSYTWREYYDLTLKFCKSLLSLGFPAHGAINLIGFNSPEWLIANCGAIAAGGVGVGIYTSNGVDACKYITEHSEAEVVVVENAKQLEKYLKIAKELPRLKALVIYSGTAEGY-----KCDVPIYSWKDFMALGSGVKDEAVRARIEAQRPGHCCTLIYTSGTTGPPKAVMISHDNLTWTVKNFVASLPFTLTCE-DRSVSYLPLSHVAAQMLDIHCPIATGAKIYFAQPDALRGSLPVTLKDVCPTYFFGVPRVWEKIYEKMQEVARSTTGVKRALAQWAKAKGLEKNRRQQYGCGGGAPVGFGCAHALVLSKVKAALGLHETKMCITSAAPIAVEILEYFASLDIPVLELFGQSECTGPHTSNFSYAWKIGSIGRDIPGVKTKQHANMSEFCMYGRHIMMGYMKMEDKTQEAVDNEGWLHSGDVAQVDAD----------GFWSITGRIKELIITAGGENIPPVLIENEIMSALPAVANCMVVGDKKKFLTVLLTMKAKLD-DQGNPTKELNKEALDIGKEIGSNASTTEQVASDPHWKKYFDEGLKKANSTATSNAQFVQKWNVLPLDFSEKGGELTPTLKLKRSVVAEKYADVIADMYK 648          

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: A0A7S2K6B1_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2K6B1_9STRA)

HSP 1 Score: 652 bits (1682), Expect = 3.750e-225
Identity = 350/673 (52.01%), Postives = 455/673 (67.61%), Query Frame = 1

Query:   82 TEPNAYLEPKIGAKGTVAGEPARTVWQMLAETVDNFPNRPALCFK--EIGGQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKA------------SRGNGAGRGGAAPRAMIRVYSWAEFMNLGETGSN---QEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTMSMDFTL---MCENDRAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKMADN-GEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEPDTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMY 2037
            ++P   +E +IG  G  A EP +TV  +       F N PAL  K   +  + A + P+T  T+ +Y E++    KS+I+LG + H C NIIGFNS  W  A FG++AAGG+ AGIY TN  EA  Y++ HS+A VV +E  +QL KY E+ A + +LKA            S GNGA          + VY + EF+ LG+  ++     V   +D Q+PG   +LIYTSGTTG PKAVMI+HDN+TWT K M ++ TL   +  +D  +S+LPLSHIAAQ+LD + P+  G  ++FAQ DAL+GSLG TLKEVRPT FFGVPRVWEK+YE MQ++ +STTG+KK I+TWAK K S +N   QYG  GG+P G+G+A+ + L KV  ALGL+ C  A  GAAP+  ++ +YFAS+D+PI+E+FGQSE TG HTVN PGAWK+GT GRPLPG+E++M    GE  +  R++  GYM M++KTA+TID  GWLH+GDVV FDD +   +  G +GF+SITGRIKELIITAGGEN+ PVLIE +FKLAMPA+SNCM IGDKRKFL ++L L +E D      +++LAGE+L  SK IGS+ATT  EVM   KW  YFD GM  AN K  S AQ+VAKW VV TDF+E+GG LTPTLKLKR      ++  +E +Y
Sbjct:    6 SDPKECVEIRIGNSGIAADEP-KTVMSVFQANCRKFANAPALHQKVMHLSDKPAAETPWTTWTFKQYEEQVFSFAKSLISLGFEKHDCINIIGFNSPEWFFANFGTIAAGGIAAGIYTTNLPEACHYVSDHSKAKVVVVEGAKQLEKYTEISARLPNLKAVVVYGEKTKPMESVGNGA----------VPVYLFDEFLQLGKDAADVTDTTVQASIDAQKPGETCTLIYTSGTTGNPKAVMITHDNLTWTSKNMLVN-TLPRALTNDDNIISYLPLSHIAAQMLDMHCPIQCGCQVWFAQADALRGSLGTTLKEVRPTIFFGVPRVWEKMYEAMQKVAKSTTGVKKMISTWAKGKASAKNIAGQYGESGGSPWGYGIASKI-LGKVHVALGLERCLCAFTGAAPIEVKILNYFASIDLPIYELFGQSECTGPHTVNFPGAWKVGTCGRPLPGTESRMEPGTGELSYTGRHIFAGYMYMDDKTADTIDSGGWLHSGDVVSFDDCSTPGI-EGPSGFMSITGRIKELIITAGGENVAPVLIESEFKLAMPALSNCMVIGDKRKFLSILLCLLVEVDEEGVA-SDKLAGEALDTSKRIGSSATTTAEVMTCSKWKDYFDKGMAVANDKTISRAQRVAKWAVVPTDFTEKGGELTPTLKLKRSVAEAKYSEIVEGIY 663          

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: G9BBC7_9STRA (Long-chain acyl-coenzyme A synthetase n=1 Tax=Nannochloropsis oculata TaxID=43925 RepID=G9BBC7_9STRA)

HSP 1 Score: 646 bits (1666), Expect = 5.690e-223
Identity = 353/660 (53.48%), Postives = 445/660 (67.42%), Query Frame = 1

Query:   82 TEPNAYLEPKIGAKGTVAGEPARTVWQMLAETVDNFPNRPALCFKEIG-GQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKAS---RGNGAGRGGAAPRAMIRVYSWAEFMNLGETGSNQEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTM--SMDFTLMCENDRAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKM-ADNGEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEPDTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMYK 2040
            T  +  ++ ++  +G  A EP RTV+Q+  ETV  + +RPAL FK++  G + +   ++V  W EY +  +   KS++AL    H   NIIGFNS  W +A  G++AAGGV  GIY +N AEA  YI++HSEA VV +E+ +QL KYV++  ++  LKA     G G G     P     +YSW  FM LG+  S   V  R++ Q+PGH  +LIYTSGTTGPPKAVMISHDN+TWTVK    ++ FTL CE DR+VS+LPL H+AAQ+LD + P+  G  IYFAQ DAL+GSL  TLK+  PTYFFGVPRVWEKIYEKMQE+ RSTTG+K+ +A WAKAKG E+NR  QYG GGGAP GFG A A+VLSKVKAALGL         AAP+  EV +YFASLDIP+ E+FGQSE TG HT N   AWK+G++GR +PG + K  A   E+C   R++MMGYMKMEEKT E +D EGWLH+GDV   D D          GF +ITGRIKELIITAGGENIPPVLIE + K A+ A++NCM +GDK+KFL ++LT+  + D      TN L  E+L   K +GS ATT ++V  D  W  YFD G+  AN+ AT NAQ V KW V+  DFSE+GG LTPTLKLKR   AE +A    ++YK
Sbjct:    7 TSASGEVDLRMEKEGPGAWEP-RTVYQVFEETVQRYGDRPALHFKKVPHGGSPETTGWSVYAWREYYDLTLTFAKSLLALDFPAHGAINIIGFNSPEWLIANCGAIAAGGVGVGIYTSNNAEACNYISEHSEAEVVVVENAKQLEKYVKIAKNLPRLKALVVYDGTGEGFTCDTP-----IYSWKAFMALGKDKSEAAVRARIEAQRPGHCCTLIYTSGTTGPPKAVMISHDNLTWTVKNFVAALPFTLTCE-DRSVSYLPLPHVAAQMLDVHCPIASGAKIYFAQADALRGSLPNTLKDACPTYFFGVPRVWEKIYEKMQEVARSTTGVKRALAQWAKAKGLEKNRRQQYGGGGGAPVGFGCAYALVLSKVKAALGLHQTKICITSAAPISVEVLEYFASLDIPVLELFGQSECTGPHTSNFSYAWKIGSIGRDIPGVKTKQEAAAKEFCMFGRHIMMGYMKMEEKTKEAVDEEGWLHSGDVADVDAD----------GFWTITGRIKELIITAGGENIPPVLIETEVKAALHAVANCMVVGDKKKFLTVLLTMKTKLD-EQGNPTNALNREALDIGKELGSEATTTEQVGKDPAWKKYFDEGLKKANAAATFNAQFVQKWAVLPLDFSEKGGELTPTLKLKRSVVAEKYADVTANLYK 648          

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: A0A7S3VDL2_9STRA (Hypothetical protein n=8 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3VDL2_9STRA)

HSP 1 Score: 601 bits (1550), Expect = 3.320e-205
Identity = 318/648 (49.07%), Postives = 426/648 (65.74%), Query Frame = 1

Query:  109 KIGAKGTVAGEPARTVWQMLAETVDNFPNRPALCFKEI-GGQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKASRGNGAGR--GGAAPRAMIRVYSWAEFMNLGETGSNQEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTMSMDFTLMCEND-RAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKM-ADNGEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEPDTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMY 2037
            KI     +AG   +T+ Q     V+ F  +PAL  K +  G++A D P+T  T+ EYR  +    KS++++G +     NI+GFN+  WH A FG++ AGG+ AGIY TNG +  KYI++HSEA VV +E  +QL KY  M + +  LKA    G        + +  + VY++A+F+ LGE+  +  + +  + Q PG +++LIYTSGTTGPPKAVMI+HDNITWT            +ND   +S+LPLSHIAAQ+LD + PM  G  I+FAQPDAL+GSLG TLK+VRPT FFGVPRVWEKIY+KMQ +G+STTG KK ++TWAK + +E     Q+      P  + +A  ++L KV+ ALGLD C    V AAP+  ++ +YFAS+DIPI E+FGQSE +G H  N+P  WK+G++GRPL G+ +K+  +NGE  +  R+V  GYM ME+KT ET+D +G+LH+GDVV  DD   + V +   GF+ ITGRIKE+IITAGGENIPPVLIE+Q K AMPA+SN M IGDKRKFL ++  L +E +      T +L GE+L  SK IGS+ATT  E    +KW  YFD GM  AN  A S AQ V KW ++  DF+E GG LTPTLKLKR   A+ ++  IE+ Y
Sbjct:   19 KIRLGEGLAGVEPQTIIQAFDNVVEKFGEKPALHQKIMKAGESAADTPWTTWTFQEYRNNVDSFAKSLLSIGFEKFDMVNILGFNAPEWHFANFGAIMAGGIAAGIYSTNGPDGCKYISEHSEAKVVVVEGVKQLEKYYSMASDLSHLKAIVMYGPDELPADVSQKLSVPVYTFADFLKLGESVLDSALTDIGEAQNPGDVTTLIYTSGTTGPPKAVMITHDNITWTANAQLKTMGRPLDNDDHMISYLPLSHIAAQMLDMHCPMQRGVQIWFAQPDALRGSLGATLKDVRPTIFFGVPRVWEKIYDKMQIVGKSTTGFKKTMSTWAKGQAAEYWENHQFEGNQSKPLLYPIAQ-LLLGKVRVALGLDRCMACYVSAAPIEVKILEYFASIDIPILELFGQSECSGPHCTNSPSGWKIGSIGRPLEGTISKLDPNNGELIYSGRHVFAGYMSMEDKTKETVDSDGFLHSGDVVSIDDCLQNGVPN--TGFMHITGRIKEIIITAGGENIPPVLIEDQMKAAMPALSNAMAIGDKRKFLTILFCLQVEINEETGMPTEKLTGEALETSKKIGSSATTTIEAQTCDKWKKYFDDGMKVANGNAASRAQNVGKWALLDGDFTEPGGELTPTLKLKRSVAADKYSEVIEAFY 663          

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: B7FXX6_PHATC (Long chain acyl-coa synthetase n=3 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7FXX6_PHATC)

HSP 1 Score: 597 bits (1539), Expect = 1.470e-203
Identity = 325/659 (49.32%), Postives = 431/659 (65.40%), Query Frame = 1

Query:   82 TEPNAYLEPKIGAKGTVAGEPARTVWQMLAETVDNFPNRPALCFKEIG-GQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKA--SRGNGAGRGGAAPRAMIRVYSWAEFMNLGETGSNQEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTMSMDFT---LMCENDRAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKMAD-NGEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEPDTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMY 2037
            +E +  +  ++G +G +A  P +TV Q     V    NR AL  K    GQ   DV +T  +W EYR+++   GK+++ +G       NIIGFN+  W  A FG++AAGG+PAG+Y TN  EA  Y+ +HSEA VV  E  +QL KY E+  ++ +L A    G  +       +  + VY++  F++LG+  S+ ++  R D  + G   +LIYTSGTTGPPKAVMI++DNITWT++T+ M  T    +  ND  +S+LPLSHIAAQ+LD + PM  G  +YFA  +ALKGSLG TLKEVRPT FFGVPRVWEKIY+K+QE+ RS+TG+KK ++TWAK K +      +YG+  G+P    +A  + L K   ALG D C    V AAP+  ++  YF SLDIPI E+FGQSE TG H VN   A+K+GTVGRPL G+E K+ +  GE C+R R++  GYM M +KT ETID +GWLH+GD+V  D D    +    +GF+SITGRIKELIITAGGEN+ PVLIE++ K AMPA++N M IGDKRKFL ++L L +EPD  +   TN+L G +L  +K IGS A T +E   D KW  YFD G+  AN KA S A +V KW ++ TDFSE GG LTPT+KLKR  TAE FA  I++MY
Sbjct:    9 SEASQEVAIRMGNEG-IASLPPKTVMQNFDAQVTKNGNRTALYHKRPKQGQDVADVAWTKYSWKEYRQQVDSFGKALLKVGFARFDTINIIGFNAPEWFFANFGAIAAGGIPAGVYTTNNPEACAYVAEHSEAKVVVCEGVKQLEKYYEISRNLPNLTALVMYGTDSIPEDVKSKCSVPVYTFEGFLDLGKDVSDTDLKARTDSWKAGETCTLIYTSGTTGPPKAVMITNDNITWTIETL-MGRTRKGTLDHNDVMISYLPLSHIAAQMLDMHNPMATGTQLYFADANALKGSLGQTLKEVRPTVFFGVPRVWEKIYDKLQEVARSSTGIKKMLSTWAKGKAAAHWESLEYGSKSGSPFMLFLAKKL-LHKAHLALGFDRCIQFYVSAAPIEVKILKYFMSLDIPIMELFGQSECTGPHAVNGYDAFKVGTVGRPLIGTETKIDEATGELCYRGRHIFAGYMGMPDKTTETIDSQGWLHSGDIVAIDSDHHPEIKKP-SGFISITGRIKELIITAGGENVAPVLIEDELKAAMPALANAMVIGDKRKFLSVLLCLQVEPDI-EGNPTNKLTGNALEAAKTIGSKAKTTNEARDDPKWKEYFDKGLEVANKKAISRASRVGKWALLSTDFSEVGGELTPTMKLKRNVTAEKFADTIDAMY 662          

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: A0A1V9YWX5_9STRA (Long-chain-fatty-acid-CoA ligase n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YWX5_9STRA)

HSP 1 Score: 592 bits (1527), Expect = 9.840e-202
Identity = 327/664 (49.25%), Postives = 426/664 (64.16%), Query Frame = 1

Query:   70 TVWVTEPNAYLEPKIGAKGTVAGEPARTVWQMLAETVDNFPNRPALCFKEIGGQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKASRGNGAGRGGAAP---RAMIRVYSWAEFMNLGETGSNQEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTM----SMDFTLMCENDRAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKMA-DNGEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEPDTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMY 2037
            T W  E +  ++ ++G  GT+AG    TV       V    +  AL +K       KD  +  ++W  Y E+    GKS++ALG++P    +IIGFN+  W +A  G + AGG+ AGIY TN   A +YI  H+ A VV  +   QL K   +   + +LKA     A    A P   +  +  +S+A+F+ LG +  + E   RM  Q P    +LIYTSGTTG PKAVM+SHDNITWT+  M    S+    +    R VS+LPLSH+AAQ++D +LP+  G  +YFAQPDALKGSLG TLKEVRPT+F GVPRVWEKI EKM +IG+ T G+KK +A WAK KG+ +N+LAQ+GA GGAPCGFGVANAVVLSKV+AALGLDAC +  VGAAPM  E+  YF+SLDIPI+++FGQSE +G  +V  PGAWK+G+VGRPLPG+E ++  +  E  FR R+ MMGY+ ME +TA  +D +GWLH+GD    D D          GF +ITGRIKELIITAGGENIPPVL+E+  K  +P +SN M +GDKRKFL  +LTL +  D  D   T +L  ++L     + S ATT  E +   K  AY DAGM  AN++ATS AQ +AK+ V+  DFS  GG LTPTLKLKR    + +A  IE++Y
Sbjct:   20 TTW--ERDGQVQLRMGPPGTIAGRAPETVCDAFQRVVAEHGDEDALLWK-------KDGSWATLSWRAYFEQCQAFGKSLVALGMEPFDAVSIIGFNAPEWLIANVGCILAGGIAAGIYTTNNPPACQYIAAHARAVVVVCDGVAQLEKLASIADQLPALKALVVYNA----AVPAGFQCRVPCHSFADFLALGASAGSLEA--RMQAQTPNQCCTLIYTSGTTGNPKAVMLSHDNITWTIAAMLQAQSVAGVGLDHTARLVSYLPLSHVAAQMIDVHLPIAIGAKVYFAQPDALKGSLGQTLKEVRPTFFLGVPRVWEKIAEKMWDIGKQTKGVKKTLAAWAKGKGARKNQLAQFGASGGAPCGFGVANAVVLSKVRAALGLDACRFCAVGAAPMSVEILTYFSSLDIPIYDIFGQSECSGPQSVCLPGAWKIGSVGRPLPGTEWRVEPETRELVFRGRHTMMGYLAMEAETAAAVDTDGWLHSGDCATIDAD----------GFGAITGRIKELIITAGGENIPPVLLEDAIKEELPILSNVMVVGDKRKFLAALLTLRVVMD-GDGAPTTQLDAKALEVLAGLQSPATTPAEAISCAKLQAYLDAGMKRANARATSRAQSIAKFHVLPHDFSMPGGELTPTLKLKRKVVVDKYAEVIEALY 657          

BLAST of mRNA_H-akashiwo_Contig113.11.3 vs. uniprot
Match: A0A8K1FDL6_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FDL6_PYTOL)

HSP 1 Score: 590 bits (1521), Expect = 3.310e-201
Identity = 325/660 (49.24%), Postives = 430/660 (65.15%), Query Frame = 1

Query:   73 VWVTEPNAYLEPKIGAKGTVAGEPARTVWQMLAETVDNFPNRPALCFKEIGGQTAKDVPFTVMTWSEYREEIIKVGKSMIALGLQPHQCTNIIGFNSKYWHLAQFGSMAAGGVPAGIYPTNGAEATKYITQHSEAPVVFLEDNRQLAKYVEMGASIKSLKASRGNGAGRGGAAPRAMI---RVYSWAEFMNLGETGSNQEVIERMDYQQPGHLSSLIYTSGTTGPPKAVMISHDNITWTVKTMSMDFTLMCENDRAVSFLPLSHIAAQLLDFYLPMLYGGAIYFAQPDALKGSLGLTLKEVRPTYFFGVPRVWEKIYEKMQEIGRSTTGLKKKIATWAKAKGSERNRLAQYGAGGGAPCGFGVANAVVLSKVKAALGLDACNYACVGAAPMGREVFDYFASLDIPIFEVFGQSESTGTHTVNAPGAWKLGTVGRPLPGSENKMAD-NGEYCFRSRNVMMGYMKMEEKTAETIDPEGWLHTGDVVKFDDDADDRVLSGLNGFVSITGRIKELIITAGGENIPPVLIEEQFKLAMPAISNCMCIGDKRKFLGMVLTLYLEPDTSDAGFTNRLAGESLANSKAIGSAATTVDEVMGDEKWAAYFDAGMTTANSKATSNAQKVAKWVVVGTDFSEQGGMLTPTLKLKRGPTAEAFAREIESMYK 2040
            VW  E +   +  + + G  A +PA TV ++  +TVD   +  AL FK       KD  +   TW +Y +  I   KS+I+LG+QPH C NIIG NS  W     G++AAG V AGIY TNG EA  YI++H  A VV +E+   L KY+++   + SLKA         G  PR +I    VY + +F+ LGE   +  V ER+  Q PGH  +LIYTSGTTGPPKAVMISHDN TWT   +      + E +++VSFLPLSH+AAQLLD ++PM  G  IYFA PDALKG+L   LKE+RPT+FFGVPRVWEK+ EKM+EIG  TTGLKK +ATWAK KG+E+   AQ+GA G     F +AN +VLSKVK ALGL+ C     GAAP+  EV ++F SLDIPI+E+FGQSE TG H++N PG WK+G++G PL G+  ++    GE  +  R++ MGY+  E  T ET+  +GWL +GD+ +FD+D          GF+ ITGRIKELIITAGGEN+PPVLIE+  K  +PA+SN M IGDKRK+L  V T  ++ D++ A  T+RL   +L  +  IGS A TV+E +  +K+  Y +AGM  +N ++TS AQ+V K++++  DFS  GG LTPTLKLKR   A+ +A EI+SMY+
Sbjct:    4 VW--EADKIADIVVQSDGIAARKPA-TVMEIFRKTVDRRGSGRALSFK-------KDGKWVAYTWKQYYQNCIHFAKSLISLGVQPHDCVNIIGHNSPEWFFTNNGTIAAGAVIAGIYTTNGPEACHYISEHCNAKVVVVENKHHLDKYLKIADQLPSLKAL----VMWDGEVPRDIICPVPVYGFRDFLKLGEDVDDAIVEERIAAQLPGHCCTLIYTSGTTGPPKAVMISHDNFTWTSYCVLDSLYGVDETEKSVSFLPLSHVAAQLLDIHVPMHLGCEIYFAGPDALKGALVDILKEIRPTFFFGVPRVWEKVMEKMREIGGKTTGLKKTLATWAKEKGAEKTERAQFGADGRKSLSFRLANRIVLSKVKEALGLENCKLCFTGAAPISAEVINFFGSLDIPIYEIFGQSECTGPHSINLPGKWKIGSIGNPLQGTRTRIDQVTGEIQYSGRHIFMGYLNNEAATKETLQ-DGWLMSGDIGQFDED----------GFLRITGRIKELIITAGGENVPPVLIEDSLKAELPALSNIMVIGDKRKYLTFVCTFRVDMDSNGAP-TDRLDNLALKAAAEIGSPARTVEEAIACDKFKKYIEAGMKRSNDQSTSRAQRVQKFIILPRDFSIPGGELTPTLKLKRSVVAKMYAAEIDSMYE 637