mRNA_H-akashiwo_Contig1059.1.1 (mRNA) Heterosigma akashiwo CCMP452

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: A0A7S4DBK0_HETAK (Phosphoenolpyruvate carboxykinase (ATP) n=4 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DBK0_HETAK)

HSP 1 Score: 1036 bits (2678), Expect = 0.000e+0
Identity = 498/506 (98.42%), Postives = 500/506 (98.81%), Query Frame = 3

Query:  297 FVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQ-----KNFANKFVVPGRTDYTSHGPKQSAKAQFL 1799
            FVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACV GILDGSINNAEFVKDELFNFKVP+SLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQ     KNFANKFVVPGRTDYTS+GPKQSAKAQFL
Sbjct:    1 FVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVKGILDGSINNAEFVKDELFNFKVPQSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQQGMFQKNFANKFVVPGRTDYTSYGPKQSAKAQFL 506          

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: A0A7S2UVQ5_9STRA (Phosphoenolpyruvate carboxykinase (ATP) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UVQ5_9STRA)

HSP 1 Score: 822 bits (2122), Expect = 5.400e-294
Identity = 391/522 (74.90%), Postives = 443/522 (84.87%), Query Frame = 3

Query:  213 GISDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKG-PAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQKNFANKFVVPGRTDYTSHGPK 1775
            GI  P  ++HNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDK+VV+N G D    VDWNNINQ  S E+W D+ AN++ +MNKAERLYV+D + G N  T+K +RF+ E AWQ HFV+NMFIRP+ +E L  F+PDF +INSC T NPKWKEHGLNS+VYA FNL+E +AVIGGTYYGGE KKGIFALMNY LP EGIMSMHCSANKG   GDT LFFGLSGTGKTTLSAD SRYL+GDDEHGWD+DGIFNFE GCYAKTI+L EENEP+I+RAI+PDALLENN I PETNWPDFA+T+KTQNGRVSYP+YHIPN+EPSG   HP NC+FLTCDAFGVLPPV+RL   QAVYHFLSGYTAKVAGTERGI EP ATFSAGFGAAFLTLHPT YADLL +KL +HGTK WLVNTGWSGG+ GVG+RM+IK TR C++GILDGSIN A FVKD+LF F+ P +LPGVP+GVL P+E WADKAAYD TA+KLA MF  N+  K+  PG TDY+ +GP+
Sbjct:   42 GIHTPTTIFHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKYVVKNEGADQ---VDWNNINQPISEEIWADMKANTLEYMNKAERLYVYDCFCGTNKKTRKAIRFLFEKAWQMHFVKNMFIRPSVDE-LADFKPDFQVINSCGTVNPKWKEHGLNSDVYAAFNLKENLAVIGGTYYGGENKKGIFALMNYTLPQEGIMSMHCSANKGMETGDTCLFFGLSGTGKTTLSADPSRYLIGDDEHGWDDDGIFNFEQGCYAKTINLSEENEPDIYRAIKPDALLENNFIVPETNWPDFANTSKTQNGRVSYPIYHIPNFEPSGSGTHPANCVFLTCDAFGVLPPVSRLSDEQAVYHFLSGYTAKVAGTERGINEPTATFSAGFGAAFLTLHPTKYADLLVQKLKKHGTKTWLVNTGWSGGSAGVGERMSIKVTRKCISGILDGSINGASFVKDDLFGFEYPTALPGVPDGVLTPKETWADKAAYDETAKKLANMFVDNY-KKYQKPGMTDYSVYGPQ 558          

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: W7TU57_9STRA (Phosphoenolpyruvate carboxykinase (ATP) n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TU57_9STRA)

HSP 1 Score: 765 bits (1976), Expect = 7.630e-272
Identity = 360/523 (68.83%), Postives = 427/523 (81.64%), Query Frame = 3

Query:  207 KWGISDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQKNFANKFVVPGRTDYTSHGPK 1775
            K GI++P  ++ NLSYP++A HE   KEG FV+NGTFAVDTG FTGRSPKDKW+V+     SE  + W ++NQ    EV+ +LY   ++H NK ++ Y+FDGY GANP+++KKVRF+TE AWQ HFV NMFIRP + +E+ GF PDFTIIN+C+  + KWKEHG+NSEV+  FNLE+KVA+IGGT+YGGEMKKGIF++MNYWLPLEGIM MHCSANKG AGDTALFFGLSGTGKTTLSAD  RYL+GDDEHGWD+ GIFNFEGGCYAKTI+L EENEP+I+RAI+ DA+LEN  IDP T  PD+ +T+KT+NGRVSYPL+HI NYEP+ M  HPD  IFLTCDAFGVLPPVA+L   QA+YHFLSGYTAKVAGTERGITEP+ATFSA FGAAFLTLHPT YADLL++KL++HGT  +LVNTGW+GG YGVGKRM+IK TR C++ ILDGSI NA F  D +F F VP++L GV   VL PR  WADKAAYDA   KL  MFQKN+  K+V PG TDY+SHGPK
Sbjct:   37 KLGITNPTTIFRNLSYPDLAKHEEANKEGTFVANGTFAVDTGKFTGRSPKDKWIVKQ--APSEDNLWWGSVNQPMKGEVFEELYKKCVAHYNKLDKAYIFDGYCGANPSSRKKVRFLTEQAWQQHFVTNMFIRPQSTKEIEGFAPDFTIINACRVVDEKWKEHGINSEVFVAFNLEKKVAIIGGTFYGGEMKKGIFSMMNYWLPLEGIMPMHCSANKGKAGDTALFFGLSGTGKTTLSADPGRYLIGDDEHGWDDHGIFNFEGGCYAKTINLSEENEPDIYRAIKRDAMLENVYIDPTTKVPDYYNTSKTENGRVSYPLHHINNYEPTSMGDHPDKVIFLTCDAFGVLPPVAKLSPGQAMYHFLSGYTAKVAGTERGITEPQATFSACFGAAFLTLHPTKYADLLQKKLEKHGTATYLVNTGWTGGGYGVGKRMSIKNTRTCIDAILDGSIKNASFSTDPVFGFSVPKALHGVASDVLDPRTAWADKAAYDAQRLKLGAMFQKNYT-KYVTPGVTDYSSHGPK 556          

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: K3XBX7_GLOUD (Phosphoenolpyruvate carboxykinase (ATP) n=2 Tax=Pythiaceae TaxID=4782 RepID=K3XBX7_GLOUD)

HSP 1 Score: 742 bits (1915), Expect = 2.320e-262
Identity = 357/554 (64.44%), Postives = 435/554 (78.52%), Query Frame = 3

Query:  120 SALKK-AFRPTLVVRSLATASRLEQLGIAQKWGISDPRI-VYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQKNFANKFVVPGRTDYTSHGPK 1775
            +ALK  AF       + +  +R   LG+  K+G+++    V+HNLSY +IA HE   KEG F++NGT+ +DTG FTGRSPKDK++V      S K + W +IN+  +PEV++ LY     H   AE++YVFDGY GANP ++KKVR ITELAWQHHFV NMF+RP T+EE+  FEPDFTI+N+C+ +N  +KEHGLNSEV+  FN+E+ VAVIGGT+YGGEMKKGIF++MNYWLPLEGIM+MHCSANKG  GDTALFFGLSGTGKTTLSAD  R+L+GDDEHGWD++GIFNFEGGCYAKTI+L +ENEP+I+ AI+ DALLEN  + PE        T+KT+NGRVSYP+YHIPN+EP+   GHPDN +FLTCDA+GVLPPV++L   QA+YHFLSGYTAKVAGTERG+TEP ATFSA FGAAFL LHPT YADLL++KL +H T  +LVNTGW+GG YGVGKRM+IK TRAC++ ILDGSI  AEF  D LF F VP++L  VP+ VL PRE W+DKAAYDATA+KLAGMFQKNF  K+V    TDY+  GPK
Sbjct:   21 AALKNGAFASPFAAMNFSANARAAALGL-DKFGVTNANTKVHHNLSYADIAKHEDANKEGQFIANGTYTIDTGKFTGRSPKDKYIVDQ--APSNKNIWWGDINRPITPEVFDALYDKVTGHYGTAEKVYVFDGYAGANPNSRKKVRIITELAWQHHFVTNMFLRPETKEEIENFEPDFTIVNACKVTNEDYKEHGLNSEVFVAFNIEKNVAVIGGTWYGGEMKKGIFSMMNYWLPLEGIMAMHCSANKGKNGDTALFFGLSGTGKTTLSADPHRWLIGDDEHGWDDEGIFNFEGGCYAKTINLSQENEPDIYNAIKRDALLENTFVIPEXXXXXXXXTSKTENGRVSYPIYHIPNHEPTSSGGHPDNIVFLTCDAYGVLPPVSKLSDGQAMYHFLSGYTAKVAGTERGVTEPTATFSACFGAAFLPLHPTRYADLLQQKLQKHKTSVYLVNTGWTGGGYGVGKRMSIKDTRACIDAILDGSIKKAEFATDALFGFSVPKALGSVPQSVLNPREAWSDKAAYDATAKKLAGMFQKNFT-KYVSKDHTDYSPFGPK 570          

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: A0A7R9UE17_9STRA (Phosphoenolpyruvate carboxykinase (ATP) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9UE17_9STRA)

HSP 1 Score: 742 bits (1915), Expect = 6.390e-262
Identity = 364/578 (62.98%), Postives = 441/578 (76.30%), Query Frame = 3

Query:   93 NVLATAATNSALKKAFRPTLVVRSLATASRLEQLGIAQKWGISDPRI---------------VYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMN-KAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQKNFANKFVVPGRTDYTSHGPKQ 1778
            N+    ATNS L+ AF    V+R     + L  L      G S P +               +  N SY  +   E   KE    SNG  AVDTG FTGRSPKDKW+V+ P   SE  + W+++N+  +PEV+++LYA  + H N  AE++YVFDG+ GANP T+K VRF+TE+AWQ+HFVRNMFIRP +++E+  F PD+TIIN+C+ S+  WK+HGLNS+V+  FNLE+KVA+IGGTYYGGEMKKGIF++MNYWLPLEGIMSMHCSANKG  GDTALFFGLSGTGKT LSAD +RYL+GDDEHGWD DGIFNFEGGCYAKTIDL EENEPEI+RAI+ DALLEN T++   N PD+ +T+KTQNGRVSYPL+HI NY+P  M GHP+N IFLTCDAFG+LPPV++L   QA+YHFLSGYTAKVAGTERGI EP+ATFSA FGAAFLTLHPT YADLL+EKL +HGTKA+LVNTGWSGG YGVG RM IK TRACV+ IL+G I+NA    D +F F  P +LPGVPEG+L PR+ WADK AYDA   +LAGMF +N+  +F++PG TDY+++GPKQ
Sbjct:   26 NLSDNRATNSMLRSAFCQARVLRGRVGLTSLRYLATVSS-GPSHPTVADIGLNELGLTTEVPMMRNPSYETLFEEEMANKEAIATSNGAMAVDTGKFTGRSPKDKWIVKQP--PSEGNLWWSDVNRGITPEVFDELYAKVLDHYNYDAEKVYVFDGFCGANPTTRKNVRFVTEMAWQNHFVRNMFIRPKSDDEIANFAPDYTIINACRISDEDWKKHGLNSDVFVAFNLEKKVAIIGGTYYGGEMKKGIFSMMNYWLPLEGIMSMHCSANKGKNGDTALFFGLSGTGKTALSADPNRYLIGDDEHGWDQDGIFNFEGGCYAKTIDLTEENEPEIYRAIKRDALLENVTLEGPNNTPDYFNTSKTQNGRVSYPLHHIENYDPVSMGGHPNNMIFLTCDAFGILPPVSKLTPEQAMYHFLSGYTAKVAGTERGIKEPQATFSACFGAAFLTLHPTKYADLLQEKLQRHGTKAYLVNTGWSGGMYGVGSRMPIKATRACVDAILNGDIDNAPVETDPVFGFDFPTALPGVPEGLLNPRDSWADKDAYDAQRLQLAGMFAENY-KQFIIPGVTDYSAYGPKQ 599          

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: A0A097IUF2_9PHAE (Phosphoenolpyruvate carboxykinase (ATP) n=1 Tax=Desmarestia viridis TaxID=62313 RepID=A0A097IUF2_9PHAE)

HSP 1 Score: 736 bits (1901), Expect = 3.100e-260
Identity = 349/536 (65.11%), Postives = 423/536 (78.92%), Query Frame = 3

Query:  171 TASRLEQLGIAQKWGISDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMN-KAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQKNFANKFVVPGRTDYTSHGPK 1775
            T + +  LGI +  GI+ P  VY NL YPE+  HE   KEG    NGT+ V TG FTGRSPKDK+ V+     S+K +DW  +N   +PEV+ ++YA ++ H N   +++YVFDGY G +P ++K VR ITELAWQHHF +NMFIRP +E E+  F+PDFTIIN+C+  N  +KE GLNS+V   FN+EE  AVI GT+YGGEMKKGIF++MNYWLPL G+M+MHCSANKG  GDTALFFGLSGTGKTTLSAD +RYL+GDDEHGWD+DGIFNFEGGCYAKT++L  ENEPEI+ AI+ DALLEN  I  +   PD+AD ++T+N RVSYP+YHI NYEPSGM GHP++ IFLTCDAFGVLPPV+RL + QA+YHFLSGYTAKVAGTERG+TEP   FSAGFGAAFLTLHPT+YADLL++KL  HGT+ +L+NTGW+GG YGVGKRM+IK TRACV+ +LDGSINN +F  D++F F+VP  LPGVP  VL PRE W+DKA YDAT  KLAGMF++NF  KFV PG TDYT+ GPK
Sbjct:   40 TMATVSSLGIDKATGITKPTTVYRNLPYPEVFEHETKNKEGEVTKNGTYCVSTGQFTGRSPKDKYFVQQ--APSDKNLDWGKVNVGVAPEVYKNIYAKAVKHYNDNVDKMYVFDGYCGTSPKSRKNVRIITELAWQHHFAKNMFIRPESEAEVADFKPDFTIINACKVVNDDYKELGLNSDVAVVFNVEENKAVITGTWYGGEMKKGIFSMMNYWLPLNGVMAMHCSANKGKNGDTALFFGLSGTGKTTLSADPARYLIGDDEHGWDDDGIFNFEGGCYAKTVNLSHENEPEIYDAIKRDALLEN-VIIKDNGEPDYADVSRTENTRVSYPIYHIDNYEPSGMGGHPNSIIFLTCDAFGVLPPVSRLSAGQAMYHFLSGYTAKVAGTERGVTEPTPNFSAGFGAAFLTLHPTMYADLLQKKLATHGTQCFLINTGWTGGGYGVGKRMSIKATRACVDSVLDGSINNTKFFSDKVFGFEVPTELPGVPSKVLNPREAWSDKAGYDATQTKLAGMFKQNFT-KFVKPGVTDYTAFGPK 571          

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: D7FYY3_ECTSI (Phosphoenolpyruvate carboxykinase (ATP) n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FYY3_ECTSI)

HSP 1 Score: 733 bits (1893), Expect = 6.080e-259
Identity = 353/539 (65.49%), Postives = 421/539 (78.11%), Query Frame = 3

Query:  162 SLATASRLEQLGIAQKWGISDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMN-KAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQKNFANKFVVPGRTDYTSHGPK 1775
            SLAT S    LGI +  GI+ P  V+HNL YP++  HE   KEG   +NGTF V TG FTGRSPKDK+ V+     S+K +DW  IN   +P+V+ ++Y  ++ H N   + +YVFDGY G +P ++K VR ITELAWQHHF +NMFIRP TE E  GF+PDFTIIN+C+  N  +KE GLNSEV   FN+EE+ AVI GT+YGGEMKKGIF++MNYWLPL+G+M+MHCSANKG  GDTALFFGLSGTGKTTLSAD  RYL+GDDEHGWD+DGIFNFEGGCYAKTI+L  ENEPEI+ AI+ DALLEN  I  +   PD+ D +KT+N RVSYP+YHI NYEPSGM GHP++ IFLTCDAFG+LPPV+RL + QA+YHFLSGYTAKVAGTERG+TEP   FSAGFGAAFLTLHPT+YADLL++KL +HGT+ +L+NTGW+GG YGVGKRM+IK TRACV+ +LDGSI    F  D +F F+VP  LPGVP  VL PRE W+DK  YDAT  KLAGMF+ NF  KFV PG TDYTS+GPK
Sbjct:   45 SLATVS---SLGIDKATGITKPSTVHHNLPYPQVFEHETKNKEGESTANGTFCVSTGQFTGRSPKDKYFVQQ--APSDKTLDWGKINVGVAPKVFENIYNKAVKHYNDNVDSMYVFDGYCGTSPKSRKNVRIITELAWQHHFAKNMFIRPETEAETAGFKPDFTIINACKVVNDDYKELGLNSEVAVVFNVEERKAVITGTWYGGEMKKGIFSMMNYWLPLDGVMAMHCSANKGKNGDTALFFGLSGTGKTTLSADPERYLIGDDEHGWDDDGIFNFEGGCYAKTINLSHENEPEIYDAIKRDALLENVVI-KDNGEPDYTDVSKTENTRVSYPIYHIDNYEPSGMGGHPNSVIFLTCDAFGILPPVSRLSAGQAMYHFLSGYTAKVAGTERGVTEPTPNFSAGFGAAFLTLHPTMYADLLQKKLAKHGTQCYLINTGWTGGGYGVGKRMSIKATRACVDAVLDGSIGKTTFTPDPVFGFEVPAELPGVPAKVLNPREAWSDKKGYDATRTKLAGMFKDNFT-KFVKPGVTDYTSYGPK 576          

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: H3G8U5_PHYRM (Phosphoenolpyruvate carboxykinase (ATP) n=24 Tax=Peronosporaceae TaxID=4777 RepID=H3G8U5_PHYRM)

HSP 1 Score: 731 bits (1887), Expect = 6.450e-259
Identity = 341/516 (66.09%), Postives = 413/516 (80.04%), Query Frame = 3

Query:  228 RIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNKAERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQKNFANKFVVPGRTDYTSHGPK 1775
            R ++ NLSY +IA HE    EG FV NGT+ +DTG FTGRSPKDK++V      S K + W +INQ  S E++++LY     H   AE++YVFDGY GANPA++KKVRFITELAWQHHFV NMF+RP TEEE+  F+PDFTI+N+C+ +N  +K+HGLNSEV+  FN+E+ VAVIGGT+YGGEMKKGIF++MNYWLPL+GIM+MHCSANKG  GDTALFFGLSGTGKTTLSAD  RYL+GDDEHGWD+DGIFNFEGGCYAKTI+L  ENEP+I+ AI+ DALLEN  +D +T  PDF +T+KT+NGRVSYP+YHIPN+EP+   GHP N +FLTCDA+GVLPPV++L   QA+YHFLSGYTAKVAGTERG+TEP ATFSA FGAAFL LHPT YADLL++KL +H T  +LVNTGW+ G YGVGKRM+IK TRAC++ ILDGSI ++EF +D  F F VP+ L  +PE VL PRE W DK AYD TA+KLA MF+ NF  K+V    TDY+  GP+
Sbjct:    7 RAMHRNLSYDDIAAHEEKNGEGQFVKNGTYTIDTGKFTGRSPKDKYIVDQ--APSSKNIWWGDINQPVSSEIFDELYETVTKHYGSAEKVYVFDGYAGANPASRKKVRFITELAWQHHFVTNMFLRPQTEEEIADFKPDFTIVNACKVTNQDYKKHGLNSEVFVAFNIEKDVAVIGGTWYGGEMKKGIFSMMNYWLPLDGIMAMHCSANKGKKGDTALFFGLSGTGKTTLSADPHRYLIGDDEHGWDDDGIFNFEGGCYAKTINLSAENEPDIYNAIKRDALLENTFVDADTKEPDFYNTSKTENGRVSYPIYHIPNHEPTSSGGHPSNIVFLTCDAYGVLPPVSKLSDGQAMYHFLSGYTAKVAGTERGVTEPTATFSACFGAAFLPLHPTKYADLLQKKLQKHNTSVYLVNTGWTSGGYGVGKRMSIKDTRACIDAILDGSIKDSEFTQDANFGFDVPKKLGSIPENVLNPREAWTDKEAYDVTAKKLANMFKTNF-KKYVSADFTDYSKFGPQ 519          

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: A0A097IUG4_PETFA (Phosphoenolpyruvate carboxykinase (ATP) n=1 Tax=Petalonia fascia TaxID=2893 RepID=A0A097IUG4_PETFA)

HSP 1 Score: 733 bits (1891), Expect = 9.850e-259
Identity = 350/539 (64.94%), Postives = 424/539 (78.66%), Query Frame = 3

Query:  162 SLATASRLEQLGIAQKWGISDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNK-AERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQKNFANKFVVPGRTDYTSHGPK 1775
            SLAT S    LGI +  GI+ P  VY+NL YPE+  HE   +EG   +NGT+ V TG FTGRSPKDK+ V+     S+K +DW  IN+  +PEV+ ++Y  ++ H N+  +++YVFDGY G +P ++K VR ITELAWQHHF +NMFIRP +E E  GFEPDFTIIN+C+  N  +K+ GLNS+V   FN+EE +AVI GT+YGGEMKKGIF++MNYWLPL+G+M+MHCSANKG  GDTALFFGLSGTGKTTLSAD  R L+GDDEHGWD+DGIFNFEGGCYAKTI+L  +NEPEI+ AI+ DALLEN  I  E   PD+ DT++T+N RVSYP+YHI N+EPSGM GHP++ IFLTCDAFGVLPPV+RL   QA+YH+LSGYTAKVAGTERG+TEP   FSAGFGAAFLTLHPT+YADLL+ KLD+HGT+ +L+NTGW+GG YGVGKRM+IK TRACVN +LDGSIN A F  D +F F VP  LPGVP  VLIPR+ W+DK +YD T  KLAGMF+ N+  KFV PG T+YTS GPK
Sbjct:   39 SLATVS---SLGIDKATGITKPSTVYYNLPYPEVFEHETKNEEGESTANGTYCVSTGQFTGRSPKDKYFVKQ--APSDKKLDWGKINKGVAPEVYENIYKKAVKHYNENVDKMYVFDGYCGTSPKSRKNVRIITELAWQHHFAKNMFIRPDSEAETAGFEPDFTIINACKVINEDYKDLGLNSDVAVVFNVEENMAVITGTWYGGEMKKGIFSMMNYWLPLDGVMAMHCSANKGKDGDTALFFGLSGTGKTTLSADPERLLIGDDEHGWDDDGIFNFEGGCYAKTINLSRDNEPEIYDAIKRDALLEN-VIIKENGEPDYTDTSRTENTRVSYPIYHIDNHEPSGMGGHPNSIIFLTCDAFGVLPPVSRLSPGQAMYHYLSGYTAKVAGTERGVTEPTPNFSAGFGAAFLTLHPTLYADLLQHKLDRHGTQCFLINTGWTGGGYGVGKRMSIKATRACVNAVLDGSINKASFTPDPVFGFDVPTELPGVPTKVLIPRDAWSDKKSYDTTRTKLAGMFKDNYT-KFVKPGVTNYTSFGPK 570          

BLAST of mRNA_H-akashiwo_Contig1059.1.1 vs. uniprot
Match: A0A097IUF6_9PHAE (Phosphoenolpyruvate carboxykinase (ATP) n=1 Tax=Punctaria latifolia TaxID=90013 RepID=A0A097IUF6_9PHAE)

HSP 1 Score: 729 bits (1881), Expect = 2.710e-257
Identity = 355/571 (62.17%), Postives = 434/571 (76.01%), Query Frame = 3

Query:   72 NSSVGIMNVLATAATNSALKKAFRPTLVVR--SLATASRLEQLGIAQKWGISDPRIVYHNLSYPEIALHEAHYKEGHFVSNGTFAVDTGIFTGRSPKDKWVVRNPGTDSEKLVDWNNINQEASPEVWNDLYANSISHMNK-AERLYVFDGYVGANPATQKKVRFITELAWQHHFVRNMFIRPTTEEELVGFEPDFTIINSCQTSNPKWKEHGLNSEVYACFNLEEKVAVIGGTYYGGEMKKGIFALMNYWLPLEGIMSMHCSANKGPAGDTALFFGLSGTGKTTLSADSSRYLLGDDEHGWDNDGIFNFEGGCYAKTIDLKEENEPEIFRAIRPDALLENNTIDPETNWPDFADTTKTQNGRVSYPLYHIPNYEPSGMAGHPDNCIFLTCDAFGVLPPVARLDSNQAVYHFLSGYTAKVAGTERGITEPEATFSAGFGAAFLTLHPTVYADLLKEKLDQHGTKAWLVNTGWSGGAYGVGKRMAIKTTRACVNGILDGSINNAEFVKDELFNFKVPRSLPGVPEGVLIPREGWADKAAYDATAEKLAGMFQKNFANKFVVPGRTDYTSHGPK 1775
            ++ V + N L  AA     +K       VR  SLAT S    LGI +  GI+ P  VYHNL YP++  HE   KEG   +NGT+ V TG FTGRSPKDK+ V+     S++ +DW  IN   +P+VW ++Y  ++ H N+  E++YVFDGY G +P ++K VR ITELAWQHHF +NMFIRP TE E  GF+PDFTIIN+C+  N  +KE GLNSEV   FN+EE+ AVI GT+YGGEMKKGIF++MNYWLPL G+M+MHCSANKG  GDTALFFGLSGTGKTTLSAD  R L+GDDEHGWDNDGIFNFEGGCYAKT++L  E EPEIF AI+ DALLEN  I  E   PD+ D +KT+N RVSYP+YHI N+EP+GM GHP++ IFLTCDAFG+LPPV+RL + QA+YHFLSGYTAKVAGTERG+TEP   FSA FGAAF+TLHPT YADLL++KL +HGT+ +L+NTGW+GG YGVGKRM++K TRACV+ +LDG+I+N  F  D +F F+VP  +PGVP+ VL PR+ W+DK  YDAT  KLAGMF++NF  KFV PG TDYTS+GPK
Sbjct:    2 SAMVSLRNSLRAAARPVLSRKITMHQQFVRTASLATVS---SLGIDKATGITKPSTVYHNLPYPQVFEHETKNKEGETTANGTYCVSTGQFTGRSPKDKYFVQQ--APSDETLDWGKINVGVAPKVWENIYKKAVKHYNENVEKMYVFDGYCGTSPKSRKNVRIITELAWQHHFAKNMFIRPDTEAETEGFKPDFTIINACKVVNDDYKELGLNSEVAVVFNVEERKAVITGTWYGGEMKKGIFSMMNYWLPLNGVMAMHCSANKGKNGDTALFFGLSGTGKTTLSADPDRKLIGDDEHGWDNDGIFNFEGGCYAKTVNLSHETEPEIFDAIKRDALLENVVI-KENGEPDYTDVSKTENTRVSYPIYHIDNFEPTGMGGHPNSVIFLTCDAFGILPPVSRLSAGQAMYHFLSGYTAKVAGTERGVTEPVPNFSACFGAAFMTLHPTKYADLLQKKLAKHGTQCFLINTGWTGGGYGVGKRMSLKATRACVHAVLDGTIHNTSFTPDPVFGFEVPAEVPGVPDKVLDPRDAWSDKDGYDATRTKLAGMFKENFT-KFVKPGVTDYTSYGPK 565