mRNA_H-akashiwo_Contig1056.2.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig1056.2.1
Unique NamemRNA_H-akashiwo_Contig1056.2.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: A0A7S1FS45_9STRA (Hypothetical protein n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1FS45_9STRA)

HSP 1 Score: 243 bits (621), Expect = 2.300e-77
Identity = 128/213 (60.09%), Postives = 151/213 (70.89%), Query Frame = 1
Query:   13 VIVPVVSLAALPVMMALFGASPAEAAEGAEPISSAFAAYGHYASILVWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSVDKAQGKLVPMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKE 651
            V+VP++ L+ L +      ASP+       P++SAFAAYGH+AS+L    A+  ER TVK NM+D EEDRLA ADI  G+AG  LAY+GYLR   +GKGWEFY HEP+FWLK+  V V GA SFFPT KI+QRS+DK  GKLVPMS  LA RM   +NAELL + SIPLTASLM+RGV Y E FPWPLGA   A + LGLG KYV EAL W E
Sbjct:   86 VLVPLLPLSVLLLPSEALAASPS-------PVASAFAAYGHFASLLAIVGALVAERLTVKPNMTDAEEDRLAAADITLGVAGTLLAYTGYLRVVSYGKGWEFYAHEPVFWLKIVFVAVFGAVSFFPTTKIVQRSLDKRNGKLVPMSEKLAGRMTSLINAELLMVGSIPLTASLMARGVGYWEDFPWPLGAAAAAAALLGLGYKYVNEALTWSE 291          
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: Q01CA3_OSTTA (Uncharacterized protein n=3 Tax=Ostreococcus TaxID=70447 RepID=Q01CA3_OSTTA)

HSP 1 Score: 222 bits (566), Expect = 3.610e-70
Identity = 114/207 (55.07%), Postives = 150/207 (72.46%), Query Frame = 1
Query:   31 SLAALPVMMALFGASPAEAAEGAEPISSAFAAYGHYASILVWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSVDKAQGKLVPMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKE 651
            ++A  P ++ +  A  AEAA   +P++SAFAAYGHY  +++   A+T ER TVKANMS++EE RL IAD + GIAG  + Y+GYLRAT++GKGWEFY HEPIFW+K+ L  V G+ S FPT+KIIQR+  KA+G++ PMS  LA R+ K +N ELLAI SIPL A+LM+RGV Y+    W  GA   A++ + LG KYVKEAL W+E
Sbjct:    4 TVAIAPAVLGMCAAGAAEAA--VDPVASAFAAYGHYLGLVLVVGALTTERLTVKANMSEDEEQRLVIADSVYGIAGVLVLYTGYLRATEYGKGWEFYAHEPIFWVKMWLFTVMGSSSLFPTIKIIQRAAAKAKGEVEPMSEKLANRITKVVNGELLAIGSIPLAAALMARGVAYAPDMSWQAGAAPIALTSVALGVKYVKEALTWEE 208          
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: A0A7S4B7A9_CHRCT (Hypothetical protein n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4B7A9_CHRCT)

HSP 1 Score: 221 bits (563), Expect = 1.440e-68
Identity = 112/202 (55.45%), Postives = 145/202 (71.78%), Query Frame = 1
Query:   55 MALFGASPAEAA-EGAEPISSAFAAYGHYASILVWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSVDKAQGKLV--PMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKE 651
            +ALF   PA+AA  GAE + SAFAAYGHY  +++ T+A+  ER  +K +M+++E D +A ADI+ G+AG  + Y+GYLR TQ+GKGWEFYQHEPIFW+K+ L  V GA SFFPT KIIQ +V K   +    PMSP L  RM   +N EL AI+ IPLTA+LM+RGV Y+++ PW   AG+ AV+  GLG KY+KEALDW E
Sbjct:   81 LALFSGLPADAAVPGAEAVPSAFAAYGHYLGLVLVTLALGTERILIKEDMTEKEFDLMANADILYGLAGTLMTYTGYLRVTQYGKGWEFYQHEPIFWVKIVLFSVLGAASFFPTTKIIQLAVSKRNPETPYKPMSPKLVARMTTILNGELSAILLIPLTATLMARGVGYADWLPWQAPAGLVAVTLFGLGGKYIKEALDWSE 282          
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: A0A7S3WSW8_9SPIT (Hypothetical protein n=1 Tax=Strombidinopsis acuminata TaxID=141414 RepID=A0A7S3WSW8_9SPIT)

HSP 1 Score: 216 bits (550), Expect = 2.210e-67
Identity = 109/184 (59.24%), Postives = 136/184 (73.91%), Query Frame = 1
Query:  106 ISSAFAAYGHYASILVWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSVDKAQGKLV--PMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKE 651
            + SAFAAYGHY S+L+   ++T +  TVK NMS+EE D+ + ADI+ G+AG  LA +GYLRATQ+GKGWEFYQHEPIFW KLTLVGV GA SFFPT K IQ ++ K        PM P L +RM   +NA+LLA+ SIPLTA+LM+RGV Y+E FPW  GA   A+S  GLG KY+KEAL+++E
Sbjct:   51 VPSAFAAYGHYLSLLLMVGSLTTQSLTVKENMSEEEFDKASNADILNGVAGLLLAVTGYLRATQYGKGWEFYQHEPIFWAKLTLVGVLGAASFFPTTKYIQLAIAKRNPDTPYRPMGPKLVKRMSTLINAQLLALASIPLTATLMARGVGYNESFPWQAGAAAVALSVFGLGYKYIKEALEFEE 234          
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: A0A0M0JMW5_9EUKA (Uncharacterized protein n=1 Tax=Chrysochromulina tobinii TaxID=1460289 RepID=A0A0M0JMW5_9EUKA)

HSP 1 Score: 214 bits (546), Expect = 5.030e-67
Identity = 111/185 (60.00%), Postives = 136/185 (73.51%), Query Frame = 1
Query:  106 ISSAFAAYGHYASILVWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSVDKAQGK---LVPMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKE 651
            I++AF AYGHY +IL+   ++T ER  VK +M+  EE  L IAD   G+AG  LA +GYLRAT FGKGWEFY HEPIFW+KLTL+ VAGA SFFPTVKIIQRSV + Q     + PMS  LA RM   +NAELLA  SIPL+A+LM+RGV Y+E+ PW +GA   A++ +GLG KY+KEALDW E
Sbjct:   33 IAAAFVAYGHYFAILLSVASLTVERLLVKPSMNMAEEQTLVIADTAYGVAGLLLAVTGYLRATSFGKGWEFYSHEPIFWVKLTLLSVAGAASFFPTVKIIQRSVAQRQTPDVPIAPMSEKLAARMTSIINAELLAFASIPLSATLMARGVGYAEWLPWQVGAAPPALALVGLGYKYIKEALDWVE 217          
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: A0A7S0KFH2_9CHLO (Hypothetical protein n=1 Tax=Ostreococcus mediterraneus TaxID=1486918 RepID=A0A7S0KFH2_9CHLO)

HSP 1 Score: 215 bits (547), Expect = 2.250e-66
Identity = 108/198 (54.55%), Postives = 143/198 (72.22%), Query Frame = 1
Query:   58 ALFGASPAEAAEGAEPISSAFAAYGHYASILVWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSVDKAQGKLVPMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKE 651
            A+  A  A+A    + ++SAFAAYGHY  +++   A+T E++TVKANM++ EE RL IAD + GIAG  + Y+GYLRAT++GKGWEFY HEPIFW+K+ L  V G+ S FPT+KIIQR+  KA+G + PMS  LA R+ K +N ELLA+ SIPL A+LMSRGV Y+    W +GA   A++ + LG KYVKEAL W+E
Sbjct:   78 AIATAYAADAHAAVDQVASAFAAYGHYLGLVLVVGALTTEKWTVKANMTEAEETRLVIADSVYGIAGILVLYTGYLRATEYGKGWEFYSHEPIFWVKMWLFTVMGSSSLFPTIKIIQRAAAKAKGDVEPMSEKLANRITKVVNGELLAVCSIPLAAALMSRGVAYAPDMSWQVGAAPIALTSVALGVKYVKEALTWEE 275          
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: A0A813DZ85_POLGL (Hypothetical protein n=3 Tax=Polarella glacialis TaxID=89957 RepID=A0A813DZ85_POLGL)

HSP 1 Score: 210 bits (534), Expect = 5.690e-64
Identity = 105/201 (52.24%), Postives = 139/201 (69.15%), Query Frame = 1
Query:   73 SPAEAAEGA-EPISSAFAAYGHYASILVWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSV---DKAQGKLVPMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKEPGLP 663
            +PAEAAE   + I  A  AYGHY ++++ T  +T ER T+K +M+ EEE R+ IAD + G+AG     +GYLR T++GKGW+FYQHEP+FW KLTLV +AGA SFF T  I++R++   D     + P++P LA RM   +NAELLAI SIPLTA+LM+RGV Y+E+ PW  GA    ++  GLG KY+KEALDW E   P
Sbjct:  110 APAEAAEATTDDIPGALVAYGHYLALVLATACLTVERLTIKPDMTKEEETRMVIADSVYGLAGLMTVVTGYLRVTEYGKGWDFYQHEPLFWFKLTLVAIAGASSFFVTAMIVKRAIARRDAGDQPIAPVTPKLANRMTSIINAELLAIGSIPLTATLMARGVGYAEWLPWQAGAAPAVLALGGLGFKYIKEALDWTEDAEP 310          
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: A0A7S0DUP2_9EUKA (Hypothetical protein n=1 Tax=Phaeocystis antarctica TaxID=33657 RepID=A0A7S0DUP2_9EUKA)

HSP 1 Score: 206 bits (523), Expect = 4.870e-63
Identity = 108/197 (54.82%), Postives = 133/197 (67.51%), Query Frame = 1
Query:   85 AAEGAEPISSAFAAYGHYASILVWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSVDKAQ---GKLVPMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKEPGLPA 666
            AA GA P  SA  AYGHY S ++ T  +T ER T+K  M+ EEE  + IAD   G+    L Y+GYLR TQ+GKGWEFYQHEPIFW+K+TL+ VAGA SFFPT  IIQR++++ +     + PMS  LA RM   +NAELLA  SIPL A+LMSRGV Y+++ PW  GA    +   GLG KYVKEALDW E  + A
Sbjct:   53 AAPGAIP--SALVAYGHYLSFMIGTACVTAERLTIKPAMTMEEEKIMTIADTTYGLTALLLVYTGYLRVTQYGKGWEFYQHEPIFWVKMTLLAVAGAASFFPTTIIIQRAIEQQKLGDTPIAPMSEKLAARMTSVLNAELLAFFSIPLAATLMSRGVGYADWLPWQAGAAPVVLVLGGLGFKYVKEALDWTEDPVDA 247          
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: A0A7S2BQQ6_9EUKA (Hypothetical protein n=1 Tax=Haptolina brevifila TaxID=156173 RepID=A0A7S2BQQ6_9EUKA)

HSP 1 Score: 205 bits (522), Expect = 6.480e-63
Identity = 108/192 (56.25%), Postives = 132/192 (68.75%), Query Frame = 1
Query:   85 AAEGAEPISSAFAAYGHYASILVWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSV---DKAQGKLVPMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKE 651
            A  GA P  SA AAYGHY   ++ +  +T ER T+K  M+ EEE  + IAD + G+A   +  +GYLR TQ+GKGWEFYQHEPIFWLKLTL+ VAGA SFFPT  IIQRS+   D     + PMSP LA RM   +NAELLA  SIPL+A+LMSRGV Y+E+ PW  GA   A++  GLG KY+ EAL W+E
Sbjct:   63 AVPGAVP--SALAAYGHYMGFILSSACLTAERLTIKPAMTVEEEKVMTIADSLYGLAALLIVGTGYLRVTQYGKGWEFYQHEPIFWLKLTLLAVAGAASFFPTTIIIQRSLKQRDAGDTPIEPMSPKLAARMTSIINAELLAFASIPLSATLMSRGVGYAEWLPWQAGAAPVALALFGLGYKYINEALTWQE 252          
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Match: A0A7S1V1F0_9STRA (Hypothetical protein n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1V1F0_9STRA)

HSP 1 Score: 204 bits (518), Expect = 6.800e-63
Identity = 108/202 (53.47%), Postives = 140/202 (69.31%), Query Frame = 1
Query:   61 LFGASPAEAAEGAE-PISSAFAAYGHYASILVWTMAITCERFTV---KANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQFGKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSVDKAQGK-LVPMSPALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVSFLGLGAKYVKEALDWKE 651
            L  ASPAEAA+ +  P++SAF AY HYAS+ V+  A+T ER  +     N+S  +E+ +  ADI+ GIA      SGY R TQ+GKGW+FYQHEPIFW+KLTLVGV GA SFFPTV + +R++    G+    +SP L +RM   MNAEL A++SIPL+A+LMSRGV Y + FPWP+GA   A++  G G KYV EAL W+E
Sbjct:    1 LMPASPAEAADASSFPVASAFVAYAHYASLFVFVGAVTTERVVIGRGAGNISQADEELMTTADIVYGIAALVATASGYFRVTQYGKGWDFYQHEPIFWVKLTLVGVLGALSFFPTVILTKRAIAFRNGEPYEELSPELVKRMTSIMNAELTALLSIPLSATLMSRGVGYWQDFPWPVGATFVALALFGAGYKYVGEALAWEE 202          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig1056.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S1FS45_9STRA2.300e-7760.09Hypothetical protein n=1 Tax=Corethron hystrix Tax... [more]
Q01CA3_OSTTA3.610e-7055.07Uncharacterized protein n=3 Tax=Ostreococcus TaxID... [more]
A0A7S4B7A9_CHRCT1.440e-6855.45Hypothetical protein n=1 Tax=Chrysotila carterae T... [more]
A0A7S3WSW8_9SPIT2.210e-6759.24Hypothetical protein n=1 Tax=Strombidinopsis acumi... [more]
A0A0M0JMW5_9EUKA5.030e-6760.00Uncharacterized protein n=1 Tax=Chrysochromulina t... [more]
A0A7S0KFH2_9CHLO2.250e-6654.55Hypothetical protein n=1 Tax=Ostreococcus mediterr... [more]
A0A813DZ85_POLGL5.690e-6452.24Hypothetical protein n=3 Tax=Polarella glacialis T... [more]
A0A7S0DUP2_9EUKA4.870e-6354.82Hypothetical protein n=1 Tax=Phaeocystis antarctic... [more]
A0A7S2BQQ6_9EUKA6.480e-6356.25Hypothetical protein n=1 Tax=Haptolina brevifila T... [more]
A0A7S1V1F0_9STRA6.800e-6353.47Hypothetical protein n=1 Tax=Grammatophora oceanic... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig1056contigH-akashiwo_Contig1056:39308..39985 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog70448.Q01CA3
PFAMsDUF2214
Model size678
Max annot lvl2759|Eukaryota
Hectar predicted targeting categorysignal peptide
Exons1
Evalue5.91e-71
EggNOG OGsCOG3556@1|root,2S1I9@2759|Eukaryota
DescriptionPredicted membrane protein (DUF2214)
Cds size678
COG categoryS
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387064.8798623-CDS-H-akashiwo_Contig1056:39307..399851681387064.8798623-CDS-H-akashiwo_Contig1056:39307..39985Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1056 39308..39985 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig1056.2.1prot_H-akashiwo_Contig1056.2.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig1056 39308..39985 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig1056.2.1

>prot_H-akashiwo_Contig1056.2.1 ID=prot_H-akashiwo_Contig1056.2.1|Name=mRNA_H-akashiwo_Contig1056.2.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=226bp
MLQPVIVPVVSLAALPVMMALFGASPAEAAEGAEPISSAFAAYGHYASIL
VWTMAITCERFTVKANMSDEEEDRLAIADIITGIAGAGLAYSGYLRATQF
GKGWEFYQHEPIFWLKLTLVGVAGACSFFPTVKIIQRSVDKAQGKLVPMS
PALAERMIKCMNAELLAIVSIPLTASLMSRGVFYSEYFPWPLGAGVFAVS
FLGLGAKYVKEALDWKEPGLPAETK*
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mRNA from alignment at H-akashiwo_Contig1056:39308..39985-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig1056.2.1 ID=mRNA_H-akashiwo_Contig1056.2.1|Name=mRNA_H-akashiwo_Contig1056.2.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=678bp|location=Sequence derived from alignment at H-akashiwo_Contig1056:39308..39985- (Heterosigma akashiwo CCMP452)
ATGCTTCAGCCAGTAATTGTACCAGTAGTTTCACTTGCAGCACTCCCTGT AATGATGGCTCTCTTTGGAGCAAGCCCTGCTGAAGCAGCCGAGGGAGCAG AGCCAATCTCAAGTGCCTTCGCAGCGTACGGCCACTATGCATCTATCCTT GTCTGGACAATGGCAATCACTTGCGAAAGATTCACAGTAAAAGCGAACAT GTCGGATGAAGAAGAGGACCGCCTGGCTATTGCAGATATCATTACTGGAA TAGCAGGCGCAGGGCTGGCGTACTCAGGCTATCTCCGTGCAACACAATTT GGTAAGGGTTGGGAGTTTTACCAGCATGAGCCAATCTTTTGGCTTAAGCT CACTTTGGTGGGGGTTGCAGGGGCATGCTCTTTCTTCCCCACTGTCAAGA TTATCCAAAGATCAGTCGATAAAGCTCAAGGAAAGCTTGTACCCATGAGC CCGGCTCTAGCTGAGCGGATGATCAAATGTATGAATGCAGAGCTGTTGGC CATCGTTTCTATCCCTCTTACTGCCTCACTCATGTCCAGAGGGGTTTTCT ACTCCGAATACTTCCCCTGGCCACTGGGGGCCGGTGTGTTTGCTGTCTCT TTTCTTGGATTGGGAGCAAAGTATGTCAAGGAGGCACTTGATTGGAAAGA ACCTGGACTACCCGCTGAGACTAAGTGA
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Coding sequence (CDS) from alignment at H-akashiwo_Contig1056:39308..39985-

>mRNA_H-akashiwo_Contig1056.2.1 ID=mRNA_H-akashiwo_Contig1056.2.1|Name=mRNA_H-akashiwo_Contig1056.2.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=678bp|location=Sequence derived from alignment at H-akashiwo_Contig1056:39308..39985- (Heterosigma akashiwo CCMP452)
ATGCTTCAGCCAGTAATTGTACCAGTAGTTTCACTTGCAGCACTCCCTGT
AATGATGGCTCTCTTTGGAGCAAGCCCTGCTGAAGCAGCCGAGGGAGCAG
AGCCAATCTCAAGTGCCTTCGCAGCGTACGGCCACTATGCATCTATCCTT
GTCTGGACAATGGCAATCACTTGCGAAAGATTCACAGTAAAAGCGAACAT
GTCGGATGAAGAAGAGGACCGCCTGGCTATTGCAGATATCATTACTGGAA
TAGCAGGCGCAGGGCTGGCGTACTCAGGCTATCTCCGTGCAACACAATTT
GGTAAGGGTTGGGAGTTTTACCAGCATGAGCCAATCTTTTGGCTTAAGCT
CACTTTGGTGGGGGTTGCAGGGGCATGCTCTTTCTTCCCCACTGTCAAGA
TTATCCAAAGATCAGTCGATAAAGCTCAAGGAAAGCTTGTACCCATGAGC
CCGGCTCTAGCTGAGCGGATGATCAAATGTATGAATGCAGAGCTGTTGGC
CATCGTTTCTATCCCTCTTACTGCCTCACTCATGTCCAGAGGGGTTTTCT
ACTCCGAATACTTCCCCTGGCCACTGGGGGCCGGTGTGTTTGCTGTCTCT
TTTCTTGGATTGGGAGCAAAGTATGTCAAGGAGGCACTTGATTGGAAAGA
ACCTGGACTACCCGCTGAGACTAAGTGA
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