mRNA_H-akashiwo_Contig1050.6.1 (mRNA) Heterosigma akashiwo CCMP452

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-akashiwo_Contig1050.6.1
Unique NamemRNA_H-akashiwo_Contig1050.6.1
TypemRNA
OrganismHeterosigma akashiwo CCMP452 (Heterosigma akashiwo CCMP452)
Homology
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: A0A7S3UUX1_HETAK (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase n=3 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UUX1_HETAK)

HSP 1 Score: 233 bits (593), Expect = 6.710e-69
Identity = 120/122 (98.36%), Postives = 120/122 (98.36%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEGATPEQVPDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP GLIGFIPEFGVLIAEINDILAEGATPEQVPDKLSVTSRAVFDLVP AIQMQLMLDRDPHGNVQV
Sbjct:  262 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLPEGLIGFIPEFGVLIAEINDILAEGATPEQVPDKLSVTSRAVFDLVPPAIQMQLMLDRDPHGNVQV 383          
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: A0A835YKH2_9STRA (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YKH2_9STRA)

HSP 1 Score: 154 bits (390), Expect = 3.000e-41
Identity = 78/124 (62.90%), Postives = 96/124 (77.42%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEGATP--EQVPDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            A+N+ALECALQTRPNMC I EEVEA  M+L  +T+ + DM+ ARAA GKHYG+VLLP GLI FIPEF  LIAEIN++LA G  P  E +   L+ T++AVF  +P  I++QL+LDRDPHGNVQV
Sbjct:  274 ASNIALECALQTRPNMCLISEEVEAKGMNLLQITHDIADMVVARAAKGKHYGMVLLPEGLIEFIPEFNHLIAEINNVLASGVEPTIEAMSAALTPTNKAVFAFLPKTIKLQLLLDRDPHGNVQV 397          
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: A0A835YX68_9STRA (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YX68_9STRA)

HSP 1 Score: 153 bits (387), Expect = 8.190e-41
Identity = 77/124 (62.10%), Postives = 95/124 (76.61%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEGATP--EQVPDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            A+N+ALECALQTRPN+C I EEVEA  M+L  +TN + DM+ ARAAAGKHYGV +LP GLI FIPEF  LIAEIN++LA G  P  + +   L+  +RAVF  +P  I++QL+LDRDPHGNVQV
Sbjct:  266 ASNIALECALQTRPNVCLISEEVEAKGMNLLQITNDIADMVVARAAAGKHYGVCMLPEGLIEFIPEFNRLIAEINNVLASGVEPSIDAMTAALTPANRAVFAFLPKTIKLQLLLDRDPHGNVQV 389          
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: A0A812VW95_SYMMI (Pfp protein (Fragment) n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812VW95_SYMMI)

HSP 1 Score: 144 bits (364), Expect = 6.010e-40
Identity = 78/125 (62.40%), Postives = 94/125 (75.20%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAA-AGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEGATP--EQVPDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            A+N+ALECALQTRPN+C I EEVEA  M+L+ +T QVVD I  R+   GK+YGVVLLP GLI FIPEF  LI++IND+LA G  P  E V  +LS  +RAVF  +P  I+ QL+LDRDPHGNVQV
Sbjct:  150 ASNIALECALQTRPNVCLISEEVEAKKMTLSEITQQVVDTIVMRSEDMGKNYGVVLLPEGLIEFIPEFNRLISDINDVLAAGTPPNEEAVLKELSYNNRAVFSYLPDNIKQQLLLDRDPHGNVQV 274          
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: D7FV23_ECTSI (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FV23_ECTSI)

HSP 1 Score: 145 bits (367), Expect = 4.050e-38
Identity = 75/124 (60.48%), Postives = 95/124 (76.61%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEG--ATPEQVPDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            A+N+ALECALQTRPN+C I EEVEA  +SL  + +++V+MI  RA AGK+YGVV+LP GLI FIPEF  LI++IND+LA G   T E V   L   +R VF+ +P +I+MQL+LDRDPHGNVQV
Sbjct:  265 ASNIALECALQTRPNVCLISEEVEAKGLSLKQIVDELVEMILKRADAGKNYGVVMLPEGLIEFIPEFNALISDINDVLASGIPTTIEGVVPALQPANRNVFNYLPQSIKMQLLLDRDPHGNVQV 388          
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: A0A7R9UAS9_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9UAS9_9STRA)

HSP 1 Score: 136 bits (342), Expect = 1.400e-37
Identity = 72/125 (57.60%), Postives = 89/125 (71.20%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEGA---TPEQVPDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            A+N+ALE ALQT PN+C I EEV     SL+ ++  +   IC RA AGK YG+VLLP GLI FIPEF +LI EINDI+A+G    T E V   LS  ++AVF  +PS I++QL+LDRDPHGNVQV
Sbjct:   80 ASNIALEVALQTGPNVCLISEEVAEREQSLSGISKAIATTICQRAQAGKDYGIVLLPEGLIEFIPEFKLLIEEINDIMAKGGVHPTEEAVMHALSFNNKAVFSYLPSDIKLQLLLDRDPHGNVQV 204          
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: L8GY22_ACACA (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GY22_ACACA)

HSP 1 Score: 137 bits (346), Expect = 1.010e-35
Identity = 68/122 (55.74%), Postives = 96/122 (78.69%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEGATPEQVPDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            A+++ LECALQTRPN+  IGEEV A   +L+ +TN++  +I  RA AGK++G+VLLP GLI F+PE   LI+E+N+++A+G + + V +KL+  SRAVFD +PSAI+ +L+LDRDPHGNVQV
Sbjct:  278 ASHITLECALQTRPNITLIGEEVAARKQTLSDLTNELCHVIVERAKAGKNFGIVLLPEGLIEFVPEVHRLISELNELMAKGNSADVVGEKLTEESRAVFDFLPSAIRHELLLDRDPHGNVQV 399          
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: W7TMW0_9STRA (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TMW0_9STRA)

HSP 1 Score: 139 bits (349), Expect = 1.080e-35
Identity = 73/124 (58.87%), Postives = 93/124 (75.00%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEGA--TPEQVPDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            A+++ALECALQTRPN   I EEVEA ++SL+ VT+ +V MI  RA  GKHYGV+LLP G+I FIPEF  L+ E+ND++A G   T E V  +LS  +RAVF  +P+ I+ QL+LDRDPHGNVQV
Sbjct:  200 ASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQV 323          
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: A0A397YQ42_BRACM (Uncharacterized protein n=2 Tax=Brassica campestris TaxID=3711 RepID=A0A397YQ42_BRACM)

HSP 1 Score: 129 bits (324), Expect = 5.980e-35
Identity = 69/124 (55.65%), Postives = 88/124 (70.97%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEGATPE--QVPDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            A+++ LECALQ  PN+  IGEEV A   +L  VT+ +VD+IC R+  G +YGV+L+P GLI FIPE  VLIAE+N+ILA     E  +   KL+  S  +FDL+P AIQ QLML+RDPHGNVQV
Sbjct:    5 ASHITLECALQAHPNITIIGEEVSAQKQTLKNVTDYMVDVICKRSELGYNYGVILIPEGLIDFIPEVQVLIAELNEILANEVVDESGEWKKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQV 128          
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Match: A0A7J6W8T1_THATH (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta n=1 Tax=Thalictrum thalictroides TaxID=46969 RepID=A0A7J6W8T1_THATH)

HSP 1 Score: 132 bits (331), Expect = 7.690e-35
Identity = 70/124 (56.45%), Postives = 88/124 (70.97%), Query Frame = 1
Query:    1 AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKHYGVVLLP-GLIGFIPEFGVLIAEINDILAEGATPEQV--PDKLSVTSRAVFDLVPSAIQMQLMLDRDPHGNVQV 363
            A+++ LECALQT PN   IGEEV A   +L +VTN + D+IC RA  G +YGV+L+P GLI FIPE   LIAE+N+ILA     E     +KL   S+A+FDL+P AIQ QL+L+RDPHGNVQV
Sbjct:   65 ASHITLECALQTHPNATIIGEEVAAKKQTLKSVTNYITDIICKRADLGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDEGGVWKNKLQPQSQALFDLLPQAIQEQLLLERDPHGNVQV 188          
The following BLAST results are available for this feature:
BLAST of mRNA_H-akashiwo_Contig1050.6.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3UUX1_HETAK6.710e-6998.36Pyrophosphate--fructose 6-phosphate 1-phosphotrans... [more]
A0A835YKH2_9STRA3.000e-4162.90Pyrophosphate--fructose 6-phosphate 1-phosphotrans... [more]
A0A835YX68_9STRA8.190e-4162.10Pyrophosphate--fructose 6-phosphate 1-phosphotrans... [more]
A0A812VW95_SYMMI6.010e-4062.40Pfp protein (Fragment) n=1 Tax=Symbiodinium microa... [more]
D7FV23_ECTSI4.050e-3860.48Pyrophosphate--fructose 6-phosphate 1-phosphotrans... [more]
A0A7R9UAS9_9STRA1.400e-3757.60Hypothetical protein (Fragment) n=1 Tax=Pinguiococ... [more]
L8GY22_ACACA1.010e-3555.74Pyrophosphate--fructose 6-phosphate 1-phosphotrans... [more]
W7TMW0_9STRA1.080e-3558.87Pyrophosphate--fructose 6-phosphate 1-phosphotrans... [more]
A0A397YQ42_BRACM5.980e-3555.65Uncharacterized protein n=2 Tax=Brassica campestri... [more]
A0A7J6W8T1_THATH7.690e-3556.45Pyrophosphate--fructose 6-phosphate 1-phosphotrans... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-akashiwo_Contig1050contigH-akashiwo_Contig1050:89240..89602 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Heterosigma akashiwo CCMP452 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D7FV23
Preferred namePFP-BETA
PFAMsPFK
Model size363
Max annot lvl2759|Eukaryota
KEGG rclassRC00017
KEGG koko:K00895
KEGG ReactionR00764,R02073
KEGG Pathwayko00010,ko00030,ko00051,ko01100,ko01110,ko01120,ko01130,map00010,map00030,map00051,map01100,map01110,map01120,map01130
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0003006,GO:0003674,GO:0003824,GO:0005575,GO:0005618,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0005975,GO:0006793,GO:0006796,GO:0007275,GO:0008150,GO:0008152,GO:0008443,GO:0009719,GO:0009725,GO:0009735,GO:0009790,GO:0009791,GO:0009793,GO:0009987,GO:0010033,GO:0010035,GO:0010038,GO:0010154,GO:0015979,GO:0016020,GO:0016301,GO:0016310,GO:0016740,GO:0016772,GO:0016773,GO:0019200,GO:0022414,GO:0030312,GO:0032501,GO:0032502,GO:0042221,GO:0044237,GO:0044238,GO:0044262,GO:0044424,GO:0044444,GO:0044464,GO:0046686,GO:0046835,GO:0047334,GO:0048316,GO:0048608,GO:0048731,GO:0048856,GO:0050896,GO:0061458,GO:0071704,GO:0071944
Exons1
Evalue6.65e-39
EggNOG OGsCOG0205@1|root,KOG2440@2759|Eukaryota
EC2.7.1.90
Description6-phosphofructokinase activity
Cds size363
COG categoryG
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681387062.8653305-CDS-H-akashiwo_Contig1050:89239..896021681387062.8653305-CDS-H-akashiwo_Contig1050:89239..89602Heterosigma akashiwo CCMP452CDSH-akashiwo_Contig1050 89240..89602 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-akashiwo_Contig1050.6.1prot_H-akashiwo_Contig1050.6.1Heterosigma akashiwo CCMP452polypeptideH-akashiwo_Contig1050 89240..89602 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-akashiwo_Contig1050.6.1

>prot_H-akashiwo_Contig1050.6.1 ID=prot_H-akashiwo_Contig1050.6.1|Name=mRNA_H-akashiwo_Contig1050.6.1|organism=Heterosigma akashiwo CCMP452|type=polypeptide|length=121bp
AANVALECALQTRPNMCFIGEEVEASNMSLAAVTNQVVDMICARAAAGKH
YGVVLLPGLIGFIPEFGVLIAEINDILAEGATPEQVPDKLSVTSRAVFDL
VPSAIQMQLMLDRDPHGNVQV
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mRNA from alignment at H-akashiwo_Contig1050:89240..89602-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-akashiwo_Contig1050.6.1 ID=mRNA_H-akashiwo_Contig1050.6.1|Name=mRNA_H-akashiwo_Contig1050.6.1|organism=Heterosigma akashiwo CCMP452|type=mRNA|length=363bp|location=Sequence derived from alignment at H-akashiwo_Contig1050:89240..89602- (Heterosigma akashiwo CCMP452)
GCCGCAAACGTTGCACTTGAGTGTGCATTGCAGACTCGCCCCAACATGTG TTTCATAGGGGAGGAGGTTGAAGCATCAAACATGAGCCTTGCAGCAGTGA CCAATCAGGTTGTTGACATGATCTGTGCCCGAGCGGCGGCTGGCAAACAC TATGGAGTGGTTCTCCTGCCCGGTCTGATTGGGTTTATCCCAGAGTTTGG CGTGCTGATTGCGGAGATCAATGATATTCTTGCAGAAGGTGCAACCCCAG AGCAAGTGCCTGACAAGCTTTCTGTCACGAGCCGTGCTGTGTTTGACCTC GTGCCATCTGCAATTCAGATGCAGCTCATGCTTGACCGCGATCCTCATGG CAACGTGCAGGTG
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Coding sequence (CDS) from alignment at H-akashiwo_Contig1050:89240..89602-

>mRNA_H-akashiwo_Contig1050.6.1 ID=mRNA_H-akashiwo_Contig1050.6.1|Name=mRNA_H-akashiwo_Contig1050.6.1|organism=Heterosigma akashiwo CCMP452|type=CDS|length=363bp|location=Sequence derived from alignment at H-akashiwo_Contig1050:89240..89602- (Heterosigma akashiwo CCMP452)
GCCGCAAACGTTGCACTTGAGTGTGCATTGCAGACTCGCCCCAACATGTG
TTTCATAGGGGAGGAGGTTGAAGCATCAAACATGAGCCTTGCAGCAGTGA
CCAATCAGGTTGTTGACATGATCTGTGCCCGAGCGGCGGCTGGCAAACAC
TATGGAGTGGTTCTCCTGCCCGGTCTGATTGGGTTTATCCCAGAGTTTGG
CGTGCTGATTGCGGAGATCAATGATATTCTTGCAGAAGGTGCAACCCCAG
AGCAAGTGCCTGACAAGCTTTCTGTCACGAGCCGTGCTGTGTTTGACCTC
GTGCCATCTGCAATTCAGATGCAGCTCATGCTTGACCGCGATCCTCATGG
CAACGTGCAGGTG
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