mRNA_H-canaliculatus_M_contigs7608.2.1 (mRNA) Hapterophycus canaliculatus Oshoro7m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_M_contigs7608.2.1
Unique NamemRNA_H-canaliculatus_M_contigs7608.2.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro7m male (Hapterophycus canaliculatus Oshoro7m male)
Homology
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LMH6_ECTSI)

HSP 1 Score: 229 bits (583), Expect = 3.360e-72
Identity = 109/116 (93.97%), Postives = 116/116 (100.00%), Query Frame = 3 
Query:    3 CYNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEEDEKGLDLGGFGVYITVIAWVMVGIGALYFLLGICCVKRIRDKKMARHKTLVAHAEMQAAMRRDHDAIA 350
            CYNILFC+LI+MAELEWTTTVREMFVLHSWIPRG+VYSFVGVLVLEEEDEKGLDLGGFG+YIT+IAW+MVGIGALYFLLG+CCVKRIRDKKMARHKTLVAHAEMQAAMRRDHDAIA
Sbjct:   56 CYNILFCMLIIMAELEWTTTVREMFVLHSWIPRGVVYSFVGVLVLEEEDEKGLDLGGFGLYITIIAWIMVGIGALYFLLGLCCVKRIRDKKMARHKTLVAHAEMQAAMRRDHDAIA 171
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBP3_9PHAE)

HSP 1 Score: 228 bits (582), Expect = 2.910e-71
Identity = 108/116 (93.10%), Postives = 116/116 (100.00%), Query Frame = 3 
Query:    3 CYNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEEDEKGLDLGGFGVYITVIAWVMVGIGALYFLLGICCVKRIRDKKMARHKTLVAHAEMQAAMRRDHDAIA 350
            CYNILFC+LI+MAELEWTTTVREMFVLHSWIPRG+VYSFVGVLVLEEEDEKGLDLGGFG+YIT++AW+MVGIGALYFLLG+CCVKRIRDKKMARHKTLVAHAEMQAAMRRDHDAIA
Sbjct:  111 CYNILFCMLIIMAELEWTTTVREMFVLHSWIPRGVVYSFVGVLVLEEEDEKGLDLGGFGLYITIVAWIMVGIGALYFLLGLCCVKRIRDKKMARHKTLVAHAEMQAAMRRDHDAIA 226
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Golgi (apparatus membrane protein TVP15 n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TYB2_9STRA)

HSP 1 Score: 89.0 bits (219), Expect = 1.030e-16
Identity = 42/112 (37.50%), Postives = 72/112 (64.29%), Query Frame = 3 
Query:    6 YNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEEDEKGLDLGGFGVYITVIAWVMVGIGALYFLLGICCVKRIRDKKMARHKTLVAHAEMQAAMRRDHD 341
            Y I+F + +V  EL WT  VR+ ++L +WI RG +Y+FVG+L +++E E    +    V+I      ++G+G LYF++G+ C+K +RD++ A +  L + AE++ A+RR  D
Sbjct:  150 YGIVFSIGVVFTELGWTQAVRDTWLLQNWISRGAIYTFVGLLSIKQEVEHEARINIVNVFIYCAGLGLIGLGVLYFVMGVFCLKGVRDRRAADYTILKSQAELEEALRRHGD 261
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Hypothetical (protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9WMH7_9STRA)

HSP 1 Score: 85.5 bits (210), Expect = 3.970e-16
Identity = 48/120 (40.00%), Postives = 76/120 (63.33%), Query Frame = 3 
Query:    6 YNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEEDEKGL----DLGGFGV---YITVIAWVMVGIGALYFLLGICCVKRIRDKKMARHKTLVAHA-EMQAAMRRDHD 341
            Y I  C +IVM ELEWT+ +RE  +L +W+ RG++Y+FVGVL +E+ +        D   F V   +I V++ +MVG+G LYF++GICC++ + +K    ++  VA A +M AA +  +D
Sbjct:   99 YIIALCAMIVMNELEWTSVIRESRLLRNWLTRGLIYAFVGVLGIEQYEASPSGRHDDSAHFDVAMNFIRVVSNIMVGVGGLYFVMGICCMQLVSNKLRHEYEERVARAGDMHAAAQTYND 218
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Cryptochrome (DASH n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D1W9_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 1.590e-15
Identity = 41/112 (36.61%), Postives = 71/112 (63.39%), Query Frame = 3 
Query:    6 YNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEEDEKGLDLGGFGVYITVIAWVMVGIGALYFLLGICCVKRIRDKKMARHKTLVAHAEMQAAMRRDHD 341
            Y I+F + +V  EL WT  VR+ ++L +WI RG +Y+FVG+L +++E E    +     +I      ++G+G LYF++G+ C+K +RD++ A +  L + AE++ A+RR  D
Sbjct:  618 YGIVFSIGVVFTELGWTQAVRDTWLLQNWISRGAIYTFVGLLSIKQEVEHEARINIVNAFIYCAGLGLIGLGVLYFVMGVFCLKGVRDRRAADYTILKSQAELEEALRRHGD 729
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Hypothetical (protein n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1FP16_9STRA)

HSP 1 Score: 77.8 bits (190), Expect = 1.730e-12
Identity = 46/110 (41.82%), Postives = 63/110 (57.27%), Query Frame = 3 
Query:    3 CYNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEEDEK------GLDLGGFGVYIT-----VIAWVMVGIGALYFLLGICCVKRIRDKKMARHKTLV 299
            CY ++FCL IV+AELEW   ++   +L  W  RGI+YSF+GVL +EE   K      G       V+I      V A V+V  G LYF++G+ C+ +IRDK    +K LV
Sbjct:  292 CYLVVFCLTIVLAELEWPGPIKRSNILQFWPFRGILYSFIGVLGMEESIVKPRTLAEGTTWSSSPVFIAHMALKVSAMVLVVTGCLYFIMGLLCINKIRDKCRQNYKELV 401
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Hypothetical (protein n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2KE92_9STRA)

HSP 1 Score: 73.6 bits (179), Expect = 2.490e-11
Identity = 41/117 (35.04%), Postives = 66/117 (56.41%), Query Frame = 3 
Query:    6 YNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEEDEKGLDL-------------GGFGVYITVIAWVMVGIGALYFLLGICCVKRIRDKKMARHKTLVAHAEMQ 317
            Y  LFC+ +++AELE    +  M +LHSW+ RGIVYSF+G++ +EE      DL             G   ++I + ++ M  +G LYFL+G  C++R+RD    ++   +  AEM+
Sbjct:  173 YMTLFCVGVIVAELE-CLLINRMPLLHSWLVRGIVYSFLGIVGMEESVAVKFDLNNDDDSSVPTTREGHASLFIKLSSYAMTALGVLYFLMGCLCMRRLRDNCRTKYNERIKKAEMK 288
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Hypothetical (protein n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4NFG8_9STRA)

HSP 1 Score: 71.6 bits (174), Expect = 3.920e-11
Identity = 37/102 (36.27%), Postives = 61/102 (59.80%), Query Frame = 3 
Query:    6 YNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEED------------EKGLDLGGFGVYITVIAWVMVGIGALYFLLGICCVKRIRDKK 275
            Y ILF L+ V+AE+E    V+ +  L +W+ RG++YSF+G++  EE              + G++     ++I V +W MVG+G LY L+GI C+K+I+D +
Sbjct:  105 YMILFTLVFVLAEVEMPFIVKHVASLQNWVLRGLMYSFIGLIGAEEARSVVVYKAVKGKLKTGMNTKMVSLFIEVASWAMVGVGLLYLLMGILCLKKIKDNR 206
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Uncharacterized (protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CNQ0_9STRA)

HSP 1 Score: 67.0 bits (162), Expect = 2.270e-10
Identity = 33/87 (37.93%), Postives = 54/87 (62.07%), Query Frame = 3 
Query:    6 YNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEEDEKGLDLGGFGVYITVIAWVMVGIGALYFLLGICCVKRIR 266
            Y I+FC+ I++AELEW + ++   +L  W  RG++Y+FVG+L LE  D     +     YI    W++VG+G LYF +G+  + ++R
Sbjct:   31 YAIVFCIGIILAELEWPSIMKNAVLLRWWPVRGLLYTFVGLLTLEISDFNDNPVAEAFHYI--FGWILVGVGVLYFFMGLARLHKVR 115
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BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Match: Uncharacterized (protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FY58_ECTSI)

HSP 1 Score: 68.2 bits (165), Expect = 5.440e-10
Identity = 30/93 (32.26%), Postives = 56/93 (60.22%), Query Frame = 3 
Query:    6 YNILFCLLIVMAELEWTTTVREMFVLHSWIPRGIVYSFVGVLVLEEEDEKGLDLGGFGVYITVIAWVMVGIGALYFLLGICCVKRIRDKKMAR 284
            Y ILF + ++ AELEW+   +    L  W  RG+ Y FVG++  ++ D        +G Y  +++++M+ +G +YFLLG+ C++ +++ + AR
Sbjct:  107 YGILFSIFVIFAELEWSKFTKFFGFLKYWPARGLFYIFVGLITWDQTDATSSS--SYGTYEDIVSFLMMSVGGIYFLLGLACMRTVKEAERAR 197
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_M_contigs7608.2.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90)
Total hits: 19
Match NameE-valueIdentityDescription
Uncharacterized3.360e-7293.97protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
Uncharacterized2.910e-7193.10protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Golgi1.030e-1637.50apparatus membrane protein TVP15 n=1 Tax=Nannochlo... [more]
Hypothetical3.970e-1640.00protein n=1 Tax=Craspedostauros australis TaxID=14... [more]
Cryptochrome1.590e-1536.61DASH n=1 Tax=Nannochloropsis salina CCMP1776 TaxID... [more]
Hypothetical1.730e-1241.82protein n=1 Tax=Corethron hystrix TaxID=216773 Rep... [more]
Hypothetical2.490e-1135.04protein n=1 Tax=Leptocylindrus danicus TaxID=16351... [more]
Hypothetical3.920e-1136.27protein n=1 Tax=Odontella aurita TaxID=265563 RepI... [more]
Uncharacterized2.270e-1037.93protein (Fragment) n=1 Tax=Tribonema minus TaxID=3... [more]
Uncharacterized5.440e-1032.26protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FY58... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_M_contigs7608contigH-canaliculatus_M_contigs7608:1445..4126 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro7m male2022-07-08
Properties
Property NameValue
Stop1
Start1
Model size900
Hectar predicted targeting categoryother localisation
Exons3
Cds size480
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696854098.5260286-UTR-H-canaliculatus_M_contigs7608:1444..18631696854098.5260286-UTR-H-canaliculatus_M_contigs7608:1444..1863Hapterophycus canaliculatus Oshoro7m maleUTRH-canaliculatus_M_contigs7608 1445..1863 -
1696854098.5901716-UTR-H-canaliculatus_M_contigs7608:4125..41261696854098.5901716-UTR-H-canaliculatus_M_contigs7608:4125..4126Hapterophycus canaliculatus Oshoro7m maleUTRH-canaliculatus_M_contigs7608 4126..4126 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696854098.5390875-CDS-H-canaliculatus_M_contigs7608:1863..21051696854098.5390875-CDS-H-canaliculatus_M_contigs7608:1863..2105Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs7608 1864..2105 -
1696854098.5519626-CDS-H-canaliculatus_M_contigs7608:3554..36751696854098.5519626-CDS-H-canaliculatus_M_contigs7608:3554..3675Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs7608 3555..3675 -
1696854098.5739944-CDS-H-canaliculatus_M_contigs7608:4008..41251696854098.5739944-CDS-H-canaliculatus_M_contigs7608:4008..4125Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs7608 4009..4125 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_M_contigs7608.2.1prot_H-canaliculatus_M_contigs7608.2.1Hapterophycus canaliculatus Oshoro7m malepolypeptideH-canaliculatus_M_contigs7608 1864..4125 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_M_contigs7608.2.1

>prot_H-canaliculatus_M_contigs7608.2.1 ID=prot_H-canaliculatus_M_contigs7608.2.1|Name=mRNA_H-canaliculatus_M_contigs7608.2.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=polypeptide|length=160bp
MLQHLVLLVDRHGGAGVDDNGARDVRASLLDSQRHRLLLRWCPGFGGGGR
ERTRPWRLWRVHHGDRVGHGGHRGPVLLVGHLLRETNSRQEDGKTQNPRG
ARGDASRHEKRSRRDRVNNRTNLYSFLFVFLSAFIFAFPCAALLFHGRCR
QPRLAYFAL*
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mRNA from alignment at H-canaliculatus_M_contigs7608:1445..4126-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_M_contigs7608.2.1 ID=mRNA_H-canaliculatus_M_contigs7608.2.1|Name=mRNA_H-canaliculatus_M_contigs7608.2.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=mRNA|length=2682bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs7608:1445..4126- (Hapterophycus canaliculatus Oshoro7m male)
GATGCTACAACATCTTGTTCTGCTTGTTGATCGTCATGGCGGAGCTGGAG
TGGACGACAACGGTGCGAGAGATGTTCGTGCTTCACTCCTGGATTCCCAG
AGGCATCGTCTACTCCTTGTGAGTGTTGGCGGCAAATAAGATCATGGCAC
ATGAAGAAAAAGAACGCTGACCGATTGGTAGAACGCCAGACTTCCTGCCA
CAGACGTTGCGAATGTGAAGACCTAAAAAACCTCGCAGCGCTACTCAGTG
CGCGTTTGCGCTGACCTGGATTTTCGGTGACGAGCGAATGAGATAGGCAG
CAGCGCGGACAAGGACCTTCAGGGACGGATGAACAATGCGAACTTGTGCA
GACGTGCGTCGCTATGGTCGAGACCGATGGCGCGTTGAACTGGACCCGCC
CCTCGCCCACTCGCTTTCGCCCGGCCGATGCACGTGACGTGCCCACTGCA
GCGTTGGTGTCCTGGTTTTGGAGGAGGAGGACGAGAAAGGACTAGACCTT
GGCGGCTTTGGCGTGTACATCACGGTGATCGCGTGGGTCATGGTGGGCAT
CGGGGCCCTGTACTTCTTGTTGGTAAGGGAGTATACGGTGGTCGGCTCGG
TTCCTGACGGGCTGCGTCTGTTTGTGACCGCGTGGAGATCTTGGGTCTCG
CCTGTCTCTGGAGGTTCGGTGCAAACGGAACGACCCAAAAATTGAAGTGA
ACAAAATACGTACTTGTATTTCAAAATTTTCTATGCTTTCGCCTCCCTTC
CCATTTTAAACCATCCCGCTACAAGCAGTCGCATTTTTTTCACGGATTCC
TGCAGCAACGAGGTAAATTCCTCAAAACATTCGACACCTCCGGACAAAGT
TTTTCACCTTGGATATAAGCACCACCAACAGCAGGAGCAACAACCGTTGC
AGTTACGAAGCAAATTTTTACCAGTGGCAATGAAGGGCCCACTGTGGGTT
GTCTACTCGATTTTATTTGTGGAGAGAAGGTCAGAGCGGAGGTTTTATTA
GGTACCGGGCGCAAAAACAGAGAGGAATGTTAGAGTAGAAGAAACGAATC
ATGTGTTCCTCCAGTTCGAGCAGAAATTCATCCCAAACTTTCAGCGGCTC
CACAGCAGTGCTTCTCACAACTGAAGGGAACCAATCAATGCGATTCAGGC
GACCAGCCTCTCACCCGCTTTCGTATACTTGCAGTTGGGTTGGAAGTTAA
AAAGCTAGTCGAGAGACTCGGTGTCCCACTCGGTCCGTGCTCTGAAACGA
GCGTGATAGGCAGGCAAGGTTCTGGCATCGACGATTGAGCACAAAATGTT
CCTTACCTTGCTTCCCCTTCATTTATGGTATCATGGTACAAGGTACGAGC
TAGTGAAATCCCCGCCTCTTCCGGCATCCTTGCGTTGCGGTTACGGTACC
TTGTTGCTGTGCTATTTGGCACTTTACTCCACGCCACTTTTGAGTCTGTT
CTGCTGCCAACCATGTGGGACGCATGCGTGCCACGTTTATTTTTCCCGAG
GCTCAAAATCCGTTGTGCTATACGTACGTGGAGGAGCAAAGTCTACAGGA
AATGGCGAGGTTGGGTTACACGTCAGTAGTAACTCTTAAGTTCTAACAAG
ATATTTTGCAGATGCCTCACACAGACCAGTAGACACACATACGTGGCCAA
CCCAACCCACCCAACCATAACCCTAAACCTAACCCTCACCGACCTCCAAC
GGGGCAGTGGTGCGAGAAACTGGCCACGAACACGGCTGCGCCGCGTCCGC
GTCGCATTCGTGGTCCGCAAGTCAGAACATGGCGCGCGCTCATTTGGCGC
ATTACAGGGCGCAAATGGCGTAGTCCTAAAGAGTTACCACTTGTGGGTAA
ACACTGCTGAATATTGTTTTGCCTCCAAGTACTACCCCCTCTTCTTTAGA
CTCTCTGCAACACGTGTTGCTCTGTTTGGTGCTCAGCGGCATCTCATGCG
ACCTAGACCACCTGATCCCGAATGTTCGCCGCCCTCAAAAACTATTTCCG
TCTCAACAACTGTGCTGCCAGGGCATCTGCTGCGTGAAACGAATTCGAGA
CAAGAAGATGGCAAGACACAAAACCCTCGTGGCGCACGCGGAGATGCAAG
CCGCCATGAGAAGAGATCACGACGCGATCGCGTGAACAATCGCACGAATT
TGTACTCGTTCCTTTTCGTGTTTCTGAGCGCGTTCATTTTTGCATTTCCT
TGCGCTGCTTTGCTTTTTCATGGTCGTTGTCGCCAGCCACGCCTTGCCTA
TTTTGCACTGTGAGTAATTTTTGTTTCATTCGAAGGCGGATCAACCGTTT
TGATAGCTGCAGCTTGTGCATCATTTAAAGTAACTCGGAGGCATGTGTTT
CCCACGATATTATTTGATGATACGCAAGCGCGTTGATAAAGGAGTTGCCT
CGTTCTCCCAGCGCACGGACGGCTCCTGTTACGGGATGGCCTAGCGGACG
ATGCGTGACCCCCGCCCTCATTGAGGGCATATGCTGCAAGCGATCACGAG
AAGATGCGAACGACAACACGGTGGCCGCTTTCCTGCCCACAGCTTCGACG
AATGAATTGGTAAACCTCATGGAGAATTCTGGCAAGGGGCACTTGCCGGG
CTAAGGTTGGTCTTCCGAGAGGAAACTATTCCTGAGAAATAACTGAAAAA
TGACTCCGCCCCCCAAAATTTCAAGTACACGG
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Coding sequence (CDS) from alignment at H-canaliculatus_M_contigs7608:1445..4126-

>mRNA_H-canaliculatus_M_contigs7608.2.1 ID=mRNA_H-canaliculatus_M_contigs7608.2.1|Name=mRNA_H-canaliculatus_M_contigs7608.2.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=CDS|length=480bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs7608:1445..4126- (Hapterophycus canaliculatus Oshoro7m male)
ATGCTACAACATCTTGTTCTGCTTGTTGATCGTCATGGCGGAGCTGGAGT
GGACGACAACGGTGCGAGAGATGTTCGTGCTTCACTCCTGGATTCCCAGA
GGCATCGTCTACTCCTTCGTTGGTGTCCTGGTTTTGGAGGAGGAGGACGA
GAAAGGACTAGACCTTGGCGGCTTTGGCGTGTACATCACGGTGATCGCGT
GGGTCATGGTGGGCATCGGGGCCCTGTACTTCTTGTTGGGCATCTGCTGC
GTGAAACGAATTCGAGACAAGAAGATGGCAAGACACAAAACCCTCGTGGC
GCACGCGGAGATGCAAGCCGCCATGAGAAGAGATCACGACGCGATCGCGT
GAACAATCGCACGAATTTGTACTCGTTCCTTTTCGTGTTTCTGAGCGCGT
TCATTTTTGCATTTCCTTGCGCTGCTTTGCTTTTTCATGGTCGTTGTCGC
CAGCCACGCCTTGCCTATTTTGCACTGTGA
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