mRNA_H-canaliculatus_M_contigs7779.1.1 (mRNA) Hapterophycus canaliculatus Oshoro7m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_H-canaliculatus_M_contigs7779.1.1
Unique Name	mRNA_H-canaliculatus_M_contigs7779.1.1
Type	mRNA
Organism	Hapterophycus canaliculatus Oshoro7m male (Hapterophycus canaliculatus Oshoro7m male)

Homology

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Uncharacterized (protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LD99_ECTSI)

HSP 1 Score: 166 bits (420), Expect = 5.210e-48
Identity = 89/122 (72.95%), Postives = 96/122 (78.69%), Query Frame = 1

Query:    1 MAIEKELSGLNRELSMFPERAFDFKNAGTVIIQQDVADVGSIVWDAEIILAHYLDQAYGSRLSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLPTLMKNARANAWDLEVGQSRPPTDG 366
            MAIE ELSGLNRELSMFPERAF+F  AG V+I+QDVADVGSIVWDAEI+LAHYLDQAYGSRLSGMRVLELGAGTGLAG+              SKLLPTL+ NARANA DLE  +  P T G
Sbjct:    1 MAIEDELSGLNRELSMFPERAFEFSKAGEVVIEQDVADVGSIVWDAEILLAHYLDQAYGSRLSGMRVLELGAGTGLAGL--------------SKLLPTLIANARANAQDLEYSRQPPTTPG 108

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Putative (methyltransferase-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CAM0_9STRA)

HSP 1 Score: 137 bits (346), Expect = 6.630e-37
Identity = 70/102 (68.63%), Postives = 82/102 (80.39%), Query Frame = 1

Query:   16 ELSGLNRELSMFPERAFDFKNAGTVIIQQDVADVGSIVWDAEIILAHYLDQAYGSRLSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLPTLMKNARAN 321
            ELS  + +L+ FP+RAF+F +AGTV+I QD  DVGSIVWDAE++LAH+LD      L GM VLELGAGTGLAGIVAARLGA VVLTDQ+KLLP L +NA AN
Sbjct:   30 ELSANDHDLARFPQRAFEFTHAGTVLIDQDFGDVGSIVWDAEVVLAHHLDATVSKSLRGMSVLELGAGTGLAGIVAARLGAHVVLTDQAKLLPQLQRNADAN 131

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Uncharacterized (protein n=2 Tax=Saprolegnia TaxID=4769 RepID=T0QEG4_SAPDV)

HSP 1 Score: 95.5 bits (236), Expect = 6.790e-22
Identity = 50/93 (53.76%), Postives = 63/93 (67.74%), Query Frame = 1

Query:   16 ELSGLNRELSMFPERAFDFKNAGTVIIQQDVADVGSIVWDAEIILAHYLDQAYGSRLSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLP 294
            ELS L+  L+   ER F F  AG+V+I QD+A VG  VWDA ++L+HYLD      + G R++ELGAGT L  I+AARLGA VV TD S +LP
Sbjct:    6 ELSTLDPSLTYARERRFHFAAAGSVVILQDIAHVGGTVWDAGVVLSHYLDTLGSIAMKGKRLVELGAGTALPSIIAARLGADVVATDMSDVLP 98

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Uncharacterized (protein n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YJ95_9STRA)

HSP 1 Score: 94.7 bits (234), Expect = 1.320e-21
Identity = 49/94 (52.13%), Postives = 63/94 (67.02%), Query Frame = 1

Query:   13 KELSGLNRELSMFPERAFDFKNAGTVIIQQDVADVGSIVWDAEIILAHYLDQAYGSRLSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLP 294
            +ELS ++R L+    R F +  AG V I QD+A VG  VWDA ++L+HYLD    + L G R++ELGAGT L  IV+ARLGA VV TD S +LP
Sbjct:    5 RELSTVDRSLTYAQTRRFVYAAAGVVTIDQDIAHVGGTVWDAGVVLSHYLDSLGSAALQGKRIIELGAGTALPSIVSARLGAVVVATDMSDVLP 98

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Uncharacterized (protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024U606_9STRA)

HSP 1 Score: 94.4 bits (233), Expect = 2.240e-21
Identity = 53/109 (48.62%), Postives = 66/109 (60.55%), Query Frame = 1

Query:    7 IEKELSGLNRELSMFPERAFDFKNAGTVIIQQDVADVGSIVWDAEIILAHYLDQAYGSRLSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLPTLMKNARANAWDL 333
            I  ELS L+R L+   ER F + NAG V I QD+ADVG  VWDA I+L+HYLD    + L G  ++ELGAGT +  IVA+RLGA  V TD  ++L       R N   L
Sbjct:    4 IRSELSFLDRNLTYAKERGFVYPNAGRVTILQDIADVGGTVWDASIVLSHYLDSLGTTVLQGKSLVELGAGTAVPSIVASRLGARAVATDMKQVLLYTEMAIRGNCDQL 112

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Methyltransferase (21A-like isoform 1 n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZGK2_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 8.200e-19
Identity = 50/109 (45.87%), Postives = 63/109 (57.80%), Query Frame = 1

Query:   16 ELSGLNRELSMFPERAFDFKNAGTVIIQQDVADVGSIVWDAEIILAHYLDQAYGSRLSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLPTLMKNARANAWDLEVG 342
            ELS + REL+   ER F+F  AG V I QD+  V   VWDA I+L+HYLD      L G  +LE+GAGT L  IV ARLGA V+ TD  + L    +   AN  +  +G
Sbjct:    2 ELSSIKRELTYAKERHFNFAIAGYVKIIQDITQVSGTVWDASIVLSHYLDSLGTITLEGKTILEIGAGTALPSIVGARLGAIVIATDLIEALEYTNRAIEANCTNNILG 110

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Aste57867_13708 (protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KZD0_9STRA)

HSP 1 Score: 87.4 bits (215), Expect = 1.250e-18
Identity = 44/92 (47.83%), Postives = 62/92 (67.39%), Query Frame = 1

Query:   16 ELSGLNRELSMFPERAFDFKNAGTVIIQQDVADVGSIVWDAEIILAHYLDQAYGSRLSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLL 291
            ELS ++R+L+   ER F+F++ G V I QD++ VG  +WDA ++L+HYLD    + LSG  ++E+GAGT L  IV ARLG  VV TD   +L
Sbjct:    8 ELSYVDRDLTYAKERHFNFEHGGHVSILQDISHVGGTIWDASVVLSHYLDSLGPATLSGKSMVEIGAGTALPSIVGARLGMDVVATDMQDVL 99

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Methyltransferase-like (protein 21D (Fragment) n=3 Tax=Parasteatoda tepidariorum TaxID=114398 RepID=A0A2L2YAX9_PARTP)

HSP 1 Score: 80.9 bits (198), Expect = 2.290e-16
Identity = 39/92 (42.39%), Postives = 62/92 (67.39%), Query Frame = 1

Query:   67 DFKNAGTVIIQQDVADVGSIVWDAEIILAHYLD--QAYGSRLSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLPTLMKNARANAWDLE 336
            ++ N    ++Q  V DVG +VWDA I++  YLD  Q + + +SG  V+++G+GTG+AG+ AA LGA V+LTD  +++P L KN ++N   L+
Sbjct:   14 EYTNVELKLVQHSVGDVGCVVWDAGIVMGKYLDYQQKFVNSMSGKMVVDIGSGTGVAGLFAAALGAKVILTDLPEIIPILEKNIQSNCKALK 105

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Hypothetical (protein n=1 Tax=Darwinula stevensoni TaxID=69355 RepID=A0A7R8XEB0_9CRUS)

HSP 1 Score: 80.1 bits (196), Expect = 4.970e-16
Identity = 44/103 (42.72%), Postives = 60/103 (58.25%), Query Frame = 1

Query:   91 IIQQDVADVGSIVWDAEIILAHYLDQAYGSR---LSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLPTLMKNARANA-------------WDLEVGQSR 351
            I+Q+ + DVG +VWDA ++LA YL++        L G +VLELGAGTGL G+VAA  G  V LTD  ++ P L +N R NA             W  ++GQS+
Sbjct:   21 IVQKTIGDVGCVVWDAALVLAKYLERLCSEEKDFLRGKKVLELGAGTGLVGLVAAYFGGDVTLTDLPEMFPLLGENVRRNALICEGRATSKVLIWGWDLGQSK 123

[Close]

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Match: Uncharacterized (protein n=2 Tax=Pteromalinae TaxID=272242 RepID=A0A7M7LJE7_NASVI)

HSP 1 Score: 78.2 bits (191), Expect = 2.760e-15
Identity = 42/78 (53.85%), Postives = 53/78 (67.95%), Query Frame = 1

Query:   94 IQQDVADVGSIVWDAEIILAHYLDQAYGSR--LSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLPTLMKNARAN 321
            +Q+ + DV  ++WDA ++LA YLD+       L G RVLELGAG G AGIVAA  GA VVLTD + +LP L KN +AN
Sbjct:   24 LQKKIGDVSCVIWDAALVLAKYLDKTSQKNKWLKGKRVLELGAGLGCAGIVAACFGAHVVLTDLATVLPMLEKNIKAN 101

The following BLAST results are available for this feature:

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.

BLAST of mRNA_H-canaliculatus_M_contigs7779.1.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
Uncharacterized	5.210e-48	72.95	protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LD99...	[more]
Putative	6.630e-37	68.63	methyltransferase-domain-containing protein n=1 Ta...	[more]
Uncharacterized	6.790e-22	53.76	protein n=2 Tax=Saprolegnia TaxID=4769 RepID=T0QEG...	[more]
Uncharacterized	1.320e-21	52.13	protein n=1 Tax=Achlya hypogyna TaxID=1202772 RepI...	[more]
Uncharacterized	2.240e-21	48.62	protein n=1 Tax=Aphanomyces invadans TaxID=157072 ...	[more]
Methyltransferase	8.200e-19	45.87	21A-like isoform 1 n=1 Tax=Thraustotheca clavata T...	[more]
Aste57867_13708	1.250e-18	47.83	protein n=1 Tax=Aphanomyces stellatus TaxID=120398...	[more]
Methyltransferase-like	2.290e-16	42.39	protein 21D (Fragment) n=3 Tax=Parasteatoda tepida...	[more]
Hypothetical	4.970e-16	42.72	protein n=1 Tax=Darwinula stevensoni TaxID=69355 R...	[more]
Uncharacterized	2.760e-15	53.85	protein n=2 Tax=Pteromalinae TaxID=272242 RepID=A0...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
H-canaliculatus_M_contigs7779	contig	H-canaliculatus_M_contigs7779:201..3209 -

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90	2022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro7m male	2022-07-08

Properties


Property Name	Value
Stop	0
Start	1
Seed ortholog	112098.XP_008610405.1
PFAMs	Methyltransf_16
Model size	366
Max annot lvl	2759\|Eukaryota
KEGG ko	ko:K21804
Hectar predicted targeting category	other localisation
Exons	3
Evalue	1.11e-22
EggNOG OGs	KOG2793@1\|root,KOG2793@2759\|Eukaryota
Description	lysine N-methyltransferase activity
Cds size	366
COG category	S
BRITE	ko00000,ko01000,ko03110

Relationships

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696854114.6456459-CDS-H-canaliculatus_M_contigs7779:200..329	1696854114.6456459-CDS-H-canaliculatus_M_contigs7779:200..329	Hapterophycus canaliculatus Oshoro7m male	CDS	H-canaliculatus_M_contigs7779 201..329 -
1696854114.658609-CDS-H-canaliculatus_M_contigs7779:1350..1485	1696854114.658609-CDS-H-canaliculatus_M_contigs7779:1350..1485	Hapterophycus canaliculatus Oshoro7m male	CDS	H-canaliculatus_M_contigs7779 1351..1485 -
1696854114.670679-CDS-H-canaliculatus_M_contigs7779:3107..3209	1696854114.670679-CDS-H-canaliculatus_M_contigs7779:3107..3209	Hapterophycus canaliculatus Oshoro7m male	CDS	H-canaliculatus_M_contigs7779 3108..3209 -

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_H-canaliculatus_M_contigs7779.1.1	prot_H-canaliculatus_M_contigs7779.1.1	Hapterophycus canaliculatus Oshoro7m male	polypeptide	H-canaliculatus_M_contigs7779 201..3209 -

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at H-canaliculatus_M_contigs7779:201..3209-
coding sequence from alignment at H-canaliculatus_M_contigs7779:201..3209-

protein sequence of mRNA_H-canaliculatus_M_contigs7779.1.1

>prot_H-canaliculatus_M_contigs7779.1.1 ID=prot_H-canaliculatus_M_contigs7779.1.1|Name=mRNA_H-canaliculatus_M_contigs7779.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=polypeptide|length=122bp
MAIEKELSGLNRELSMFPERAFDFKNAGTVIIQQDVADVGSIVWDAEIIL
AHYLDQAYGSRLSGMRVLELGAGTGLAGIVAARLGASVVLTDQSKLLPTL
MKNARANAWDLEVGQSRPPTDG

mRNA from alignment at H-canaliculatus_M_contigs7779:201..3209-

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_H-canaliculatus_M_contigs7779.1.1 ID=mRNA_H-canaliculatus_M_contigs7779.1.1|Name=mRNA_H-canaliculatus_M_contigs7779.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=mRNA|length=3009bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs7779:201..3209- (Hapterophycus canaliculatus Oshoro7m male)
ATGGCAATCGAAAAAGAGCTATCGGGGCTCAACCGCGAGCTTTCCATGTT
TCCTGAGCGAGCCTTCGACTTCAAGAATGCCGGAACTGTGATCATTCAAC
AGGTACGTACACGGGACTGAGACATTGCACTTCCTCTTCGACCGACTGCT
GCGTCCTACTTTGTAGCAGTCTGTGCCATGAACTGCTGTGGTATCGTCTG
TGCATATCATGTGTCCTTCGTAAGATCCTGGTGGCGATCATATTCCTCGT
ACATAACCGTGGTCCTGCTTCCCTGTGAAGTACTCTCCGTACATGTTATA
GTGCGATAGGACACGGCCAGTCGAAGGTGCCCGTTTGTGCTCGCAATCTT
GAGGTCGACTTCTAATTGTATGTTGTTATGTACTCCGAGCGTTGTTTCTT
CAGTCACTACTTGCAGGCGTTGTGCTTGCTTTGCTTGAGTGACGGCATGG
ATAGTCCTGGCTGCCACTGTCCAGGGAGTAGTACTACAGTAGCAGCTTTC
AGGCGGGCATTCGACTACTACTTCGAAGTAGTTGGCATGGAGACAACTAT
AAAACCCTTGTGTAGCGAGACCTCTCGGTTGGTGGCCGTCACGAGCAGCC
GACCAAGCTACGCCGACCGGTACTGGTCATGTGTAATATGAACCGAGCGA
GTGGCGACGCCTTGAACTTTTTTCTCCCTGGGGTTCCTGAGGGGGGGGAA
GGCTGTGCCAATCGGTCCCGCGTGTAGGGTGCCTCCAGCTTGATTTGAAG
TGGTCGTCAATAACAGCACTTTGGGGGAAAAGCAAGACCCACCTGTACAG
AGGACCAGATGTCGATCTGACGGCCCCTGAGAGGAGAGAAAAATACAAAA
ATACGGCACGATTCCTGAGCAACGTGTGGCGATGGTCCGACAGGAAATAG
CCGTGCATCAGGCTTTTGTCGTCAATAGCTTGAAAGTCTCAATCCTGGGG
TTTCAAAAAATACGGCCTCCTACAAAGGAAGCAGGCAGGCTCACAGGTAT
ATCCTCGGAGTGTATCGCTGTGTAGGATGGGCGTGGGAAGTTTCTGGAGG
TAGGCTGCTGGGCGTGATCGGCGGCAAAAGTCATCGGATCACAAGCGTTT
CACTGGCGAGAATGCCCCATCCGGTGCATGTAGACAGAGGGGCATCCGAC
CCGCGCTGTTCGCCGTCCCGACCAAGTCGACCACGACACGCCCCACTGCT
GCTGCGGGCATCTTCCAACAGGAAATACCACTCTCTGTGCTTGCGTCAAG
CGTTTGTGGGCGTTTCTTGCTGCTGTGGGGAAAAGAACAGGGCTAAGTCG
TTCTGCTGGGAATTTTCCGGAGCGCACCAAGAACGGCAGCAGCGACCGTA
CCAGAATCTTGTTTTCGAAGCCGAGCCGTGGGGCTCCGGGGGCCCCAACC
CCCCCCTCGGCAATTTTTTCCCACCACCAAAATATGGTAGGGGTGATCCA
AAAGAGCGCCGCCCTAAACACATGGTCTCTTACAGGTACCGTATCGCAGA
TCGACTGCTGTTTGGTAGTGAGTCCGCTCGGCTCATCACTCATCCAATTT
ACAGCAAATACAAGTATCAGGTATCAAACAAGCGTTGTTTGGGTGTTGTA
CACTCTAGGTTTAATAGCGCGGGCCTTATCTACGAATATCCGTCACCTTT
AAACGTATTGATTCCGGTGCCCCGAGGTAAAAAAATATTCCCCGTTTCGA
CACCCGTGCCCGATCGCCCGGCAGGATGTGGCCGACGTGGGGTCAATCGT
GTGGGACGCGGAGATTATTCTCGCCCACTACCTGGACCAGGCATACGGTT
CCCGGCTGAGCGGAATGCGGGTGTTGGAGCTCGGGGCTGGAACTGGACTT
GCTGGCATCGTAAGTGCATCGAGACGATCAATTTTTTCTCGAATGAACTT
CGCATAAATGCAACATAGAAATACAAGGATCTTTCGGATGCTGAACGCGT
TTTTGGAGGCTAAGTGCTTGTATCCGTCATAACCATACGATTGTTTGGTA
CGCAGTCCTGACATATTTCGGCACGTTTCTGCGACGAACCATGCATCGAC
TTGCTTCTGCCGATACCTCGAGACAGAATCCTTCTGTATCCTCTTCGTTG
TCGATGTCTACGTGTGACAGCAAAACAAGAGTGCGCGAAGAATACGGAAG
ATTTTAATGGGTGGAACCCAGTTTCCAATATCAGGTTTCCAACTGTGTCA
TGTCAAGCCTGATGTCGCGCACCCGCGATACCGTTAGCAAGAGGCAATGC
GCGCTTCCCCAACAGCGTTCATGCCTTCATCGAACCAAACAGTCAGACTG
CCAGCCCATTTTTCTGGTAACGGTAGTAGCACTGAGTGGAAGTAGATGGA
TGAGAACTACCTGAAAGGGCAGGAGCAAGCAGTTAGACTGCCAGCCCATT
TTTCTGGTAACGGTTGTAGCACTGAGTGGAAGCAGATGGATGAGAACTAC
CTGAAAGGGCAGGAGACAATGTTATTGACCCCTGATCTCATCTGTGCTGT
GTCACTCCTGGAGTAGCTTCCTTGATGTTTCAAGGAAACCAATGTTGACT
CACCTGTCGAGACTATACCTACCGGAAGAACAGTTTTATGCACACGTCGA
AAGTACAAGCATCGGTGCCCATGCTCACAAAGAGACGCCATGCCCCTCAC
GCGCGCAAATATGGCACGTTCCGAAAAATCTTCAGAAACCTCTCCCAAGG
GCAACATAAAAAAATTAGTCGCGGGTCGTGGGCACGTAATAGAGCACAGA
GTATCACCAGCAGGCAAAAAGTGAACAAAAACTACTGGTTGCCCGGTATC
CAACCCATCATCCCCGGGCTTTAACTCTCAATACGAAACTCGAACTTCAT
TTTCAACAAAATTCCTCGGTAAACACGCAGGTCGCGGCACGGCTTGGTGC
ATCCGTGGTGCTCACGGACCAGTCTAAGCTCTTGCCAACGCTGATGAAGA
ACGCGCGCGCCAACGCTTGGGATCTCGAGGTTGGCCAATCTAGGCCACCC
ACGGACGGT

Coding sequence (CDS) from alignment at H-canaliculatus_M_contigs7779:201..3209-

>mRNA_H-canaliculatus_M_contigs7779.1.1 ID=mRNA_H-canaliculatus_M_contigs7779.1.1|Name=mRNA_H-canaliculatus_M_contigs7779.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=CDS|length=366bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs7779:201..3209- (Hapterophycus canaliculatus Oshoro7m male)
ATGGCAATCGAAAAAGAGCTATCGGGGCTCAACCGCGAGCTTTCCATGTT
TCCTGAGCGAGCCTTCGACTTCAAGAATGCCGGAACTGTGATCATTCAAC
AGGATGTGGCCGACGTGGGGTCAATCGTGTGGGACGCGGAGATTATTCTC
GCCCACTACCTGGACCAGGCATACGGTTCCCGGCTGAGCGGAATGCGGGT
GTTGGAGCTCGGGGCTGGAACTGGACTTGCTGGCATCGTCGCGGCACGGC
TTGGTGCATCCGTGGTGCTCACGGACCAGTCTAAGCTCTTGCCAACGCTG
ATGAAGAACGCGCGCGCCAACGCTTGGGATCTCGAGGTTGGCCAATCTAG
GCCACCCACGGACGGT