mRNA_H-canaliculatus_M_contigs75445.1.1 (mRNA) Hapterophycus canaliculatus Oshoro7m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_H-canaliculatus_M_contigs75445.1.1
Unique Name	mRNA_H-canaliculatus_M_contigs75445.1.1
Type	mRNA
Organism	Hapterophycus canaliculatus Oshoro7m male (Hapterophycus canaliculatus Oshoro7m male)

Homology

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: Transposase (n=4 Tax=Rhodobacterales TaxID=204455 RepID=A0A196NKS5_9RHOB)

HSP 1 Score: 310 bits (795), Expect = 3.550e-104
Identity = 158/179 (88.27%), Postives = 167/179 (93.30%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKMDFVDLLAAI +IVNASKG+SMVQLSRDLDCQYKTAFVLAHKLREAMA+EVHTGEVLEGHVEIDGA+FGGHIRPENAK DRKDRRLKE+QTGKRRVVI MRER GRTLP V  +EGEGVALA ENVSRTATMSADEA HWD+LH G++VDRVNHSEIYSD GKHTNM ESFFS
Sbjct:   78 IFASRKMDFVDLLAAICIIVNASKGVSMVQLSRDLDCQYKTAFVLAHKLREAMAMEVHTGEVLEGHVEIDGAYFGGHIRPENAKADRKDRRLKEHQTGKRRVVIVMRERAGRTLPTVAMNEGEGVALAQENVSRTATMSADEASHWDMLHAGWNVDRVNHSEIYSDHGKHTNMAESFFS 256

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: Transposase (zinc-ribbon domain/ISXO2-like transposase domain n=5 Tax=Rhodobacterales TaxID=204455 RepID=A0A0J5QEM0_9RHOB)

HSP 1 Score: 306 bits (783), Expect = 1.220e-102
Identity = 158/179 (88.27%), Postives = 166/179 (92.74%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKMDFVDL AAI +IVNASKG+SMVQLSRDLDCQYKTAFVLAHKLREAMA EVHTGEVLEGHVEIDGA+FGGHIRPENAK DR DRR KE+QTGKRRVVIA+REREGRTLPFV  SEGEGVALANENVSR +TMSADEA HWD LH+G+ VDRVNHSEIYSD GKHTNMVESFFS
Sbjct:   78 IFASRKMDFVDLWAAICIIVNASKGVSMVQLSRDLDCQYKTAFVLAHKLREAMAREVHTGEVLEGHVEIDGAYFGGHIRPENAKVDRVDRRKKEHQTGKRRVVIALREREGRTLPFVGMSEGEGVALANENVSRLSTMSADEANHWDELHLGHYVDRVNHSEIYSDHGKHTNMVESFFS 256

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: Putative (transposase n=1 Tax=Roseobacter sp. CCS2 TaxID=391593 RepID=A4EIH2_9RHOB)

HSP 1 Score: 306 bits (784), Expect = 1.660e-102
Identity = 158/179 (88.27%), Postives = 165/179 (92.18%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKMDFVDLLAAIFLIVNASKG+SMVQLSRDLDCQYKTAFVLAHKLREAMA EVHTGEVL+GHVEIDGA+FGGHIRP NAK DR DRRLK NQTGKRRVV+AMRER+GRTLPFV   EGEGVALA ENVSRTATMSADEA HWD LH G+ V+RVNHSEIYSD GKHTNMVESFFS
Sbjct:   78 IFASRKMDFVDLLAAIFLIVNASKGMSMVQLSRDLDCQYKTAFVLAHKLREAMATEVHTGEVLDGHVEIDGAYFGGHIRPANAKADRVDRRLKRNQTGKRRVVVAMRERQGRTLPFVAMGEGEGVALAVENVSRTATMSADEASHWDTLHDGWMVNRVNHSEIYSDHGKHTNMVESFFS 256

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: Transposase (n=2 Tax=Rhodobacterales TaxID=204455 RepID=A0A1X7PY79_9RHOB)

HSP 1 Score: 305 bits (782), Expect = 3.460e-102
Identity = 153/179 (85.47%), Postives = 167/179 (93.30%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKMDFVDLLAAI + VNASKG+SMVQLSRDLDCQYKTAFVLAHKLREAMA EVHTGEVL+GHVEIDGA+FGGHIRPEN K+DRKDRRLK +QTGKRRVV+A+RER GRTLPFV  SEGEGVALANEN+SR AT+SADEA HWD+LH G++VDRVNHSE+YSD GKHTNMVESFFS
Sbjct:   78 IFASRKMDFVDLLAAICITVNASKGVSMVQLSRDLDCQYKTAFVLAHKLREAMAQEVHTGEVLDGHVEIDGAYFGGHIRPENRKEDRKDRRLKAHQTGKRRVVVALRERVGRTLPFVAMSEGEGVALANENISRMATISADEASHWDMLHAGWNVDRVNHSEVYSDHGKHTNMVESFFS 256

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: Putative (transposase n=1 Tax=Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) TaxID=398580 RepID=A8LJX1_DINSH)

HSP 1 Score: 304 bits (779), Expect = 9.570e-102
Identity = 152/179 (84.92%), Postives = 166/179 (92.74%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKMDFVDLLAAI ++VNASKG+SM+QLSRDLDCQYKTAFVLAHKLREAMA EV TGEVLEGHVEIDGA+FGGHIRPEN K+DRKDRRLKENQTGKRRVV+A+REREGRTLPFV K E EGVALANENV R AT+SADEA HWD+LH G+DV RVNHS++YSD GKHTN+VESFFS
Sbjct:   78 IFASRKMDFVDLLAAICILVNASKGVSMIQLSRDLDCQYKTAFVLAHKLREAMAQEVQTGEVLEGHVEIDGAYFGGHIRPENRKEDRKDRRLKENQTGKRRVVVALREREGRTLPFVTKLESEGVALANENVCRMATVSADEASHWDMLHNGWDVQRVNHSQVYSDHGKHTNLVESFFS 256

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: Possible (transposase n=5 Tax=Rhodobacterales TaxID=204455 RepID=A3W4M7_9RHOB)

HSP 1 Score: 298 bits (763), Expect = 2.580e-99
Identity = 153/179 (85.47%), Postives = 162/179 (90.50%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKMDFVDLLAAI +IVN +KG+SM+QLSRDLDCQYKTAFVLAHKLREAMA EV TGEVL+GHVEIDGA+FGGHIRPENAK DRKDRRLK +QTGKRRVVIAMRER GRTLP V  SE EGVALANENV R ATMSADEA HWD LHMG+ VDRVNHSEIYSD GKHTNMVES+FS
Sbjct:   78 IFASRKMDFVDLLAAICIIVNGAKGVSMIQLSRDLDCQYKTAFVLAHKLREAMAQEVQTGEVLQGHVEIDGAYFGGHIRPENAKADRKDRRLKAHQTGKRRVVIAMRERTGRTLPVVGMSEAEGVALANENVCRMATMSADEASHWDELHMGWSVDRVNHSEIYSDHGKHTNMVESYFS 256

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: IS1595 (family transposase n=1 Tax=Lentibacter algarum TaxID=576131 RepID=UPI001C094F1F)

HSP 1 Score: 298 bits (763), Expect = 2.580e-99
Identity = 154/179 (86.03%), Postives = 164/179 (91.62%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKMDFVDLLAAIFLIVNASKG+SMVQLSR +DCQYKTAFVLAHKLREAMALEV TGEVLEGHVEIDGA+FGGHIRP N K  R DRRLK++QTG RRVV+A+REREGRTLPF+  +EGEGVALA ENVSRTATMSADEA HWDLLH G+ VDRVNHSEIYSD GKHTNMVESFFS
Sbjct:   78 IFASRKMDFVDLLAAIFLIVNASKGMSMVQLSRSIDCQYKTAFVLAHKLREAMALEVQTGEVLEGHVEIDGAYFGGHIRPANEKAKRIDRRLKKHQTGTRRVVVALREREGRTLPFIAMNEGEGVALAVENVSRTATMSADEASHWDLLHDGWMVDRVNHSEIYSDHGKHTNMVESFFS 256

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: Putative (IS1595 family transposase n=2 Tax=Octadecabacter arcticus TaxID=53946 RepID=M9RMZ2_9RHOB)

HSP 1 Score: 297 bits (761), Expect = 6.100e-99
Identity = 151/179 (84.36%), Postives = 162/179 (90.50%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKMDFVDLLAAIF++VNASKG+SMVQLSRDLDCQYKTAFVL+HKLREAMA EV TGEVL+GH+EIDGA+F GHIRP NAK DR D RLK +QTGKRRVV+A+RER GRTLPFV  SE EGVALANENV RTATMSADEA HWD LHMG+ VDRVNHSEIYSD GKHTNMVESFFS
Sbjct:   83 IFASRKMDFVDLLAAIFILVNASKGVSMVQLSRDLDCQYKTAFVLSHKLREAMAQEVQTGEVLQGHIEIDGAYFSGHIRPANAKVDRVDLRLKRHQTGKRRVVVALRERTGRTLPFVSMSEAEGVALANENVCRTATMSADEASHWDALHMGHHVDRVNHSEIYSDHGKHTNMVESFFS 261

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: IS1595 (family transposase n=3 Tax=Roseobacteraceae TaxID=2854170 RepID=A0A365TRI5_9RHOB)

HSP 1 Score: 296 bits (759), Expect = 1.040e-98
Identity = 149/179 (83.24%), Postives = 161/179 (89.94%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKMDFVDLLAAI ++VN +KG+SM+QLSRDLDCQYKTAFVLAHKLREAMA EV TGEVLEGH+EIDGA+FGGHIRPEN K+DRKDRR K+NQTGKRRVVIAMRER GRTLPFV   EGEGVALANEN+ R ATMSADEA HWD LHMG+ VDRVNHSE YSD GKHTNM ES+FS
Sbjct:   78 IFASRKMDFVDLLAAICILVNGAKGVSMIQLSRDLDCQYKTAFVLAHKLREAMASEVQTGEVLEGHIEIDGAYFGGHIRPENRKEDRKDRRRKDNQTGKRRVVIAMRERVGRTLPFVSTDEGEGVALANENICRLATMSADEASHWDELHMGWAVDRVNHSECYSDHGKHTNMAESYFS 256

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BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Match: DDE_Tnp_IS1595 (domain-containing protein n=2 Tax=Rhodobacterales TaxID=204455 RepID=A0A2R8B9L6_9RHOB)

HSP 1 Score: 295 bits (755), Expect = 4.220e-98
Identity = 152/179 (84.92%), Postives = 161/179 (89.94%), Query Frame = 1

Query:    1 IFASRKMDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALEVHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAMREREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVDRVNHSEIYSDDGKHTNMVESFFS 537
            IFASRKM FVDLLAAI LIVNASKG+SMVQLSRD+DCQYKTAFVLAHKLREA+A EV TGEVLEGHVEIDGA+FGGHIRP N K  R DRRLK++QTG RRVV+A+RER GRTLPFV  +EGEGVALA ENVSR ATMSADEA HWD LHMGYDVDRVNHSEIYSD GKHTNMVESFFS
Sbjct:   78 IFASRKMGFVDLLAAICLIVNASKGMSMVQLSRDIDCQYKTAFVLAHKLREAIAREVQTGEVLEGHVEIDGAYFGGHIRPHNEKAKRVDRRLKKHQTGMRRVVVALRERTGRTLPFVAMTEGEGVALAQENVSRMATMSADEASHWDALHMGYDVDRVNHSEIYSDHGKHTNMVESFFS 256

The following BLAST results are available for this feature:

BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.

BLAST of mRNA_H-canaliculatus_M_contigs75445.1.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
Transposase	3.550e-104	88.27	n=4 Tax=Rhodobacterales TaxID=204455 RepID=A0A196N...	[more]
Transposase	1.220e-102	88.27	zinc-ribbon domain/ISXO2-like transposase domain n...	[more]
Putative	1.660e-102	88.27	transposase n=1 Tax=Roseobacter sp. CCS2 TaxID=391...	[more]
Transposase	3.460e-102	85.47	n=2 Tax=Rhodobacterales TaxID=204455 RepID=A0A1X7P...	[more]
Putative	9.570e-102	84.92	transposase n=1 Tax=Dinoroseobacter shibae (strain...	[more]
Possible	2.580e-99	85.47	transposase n=5 Tax=Rhodobacterales TaxID=204455 R...	[more]
IS1595	2.580e-99	86.03	family transposase n=1 Tax=Lentibacter algarum Tax...	[more]
Putative	6.100e-99	84.36	IS1595 family transposase n=2 Tax=Octadecabacter a...	[more]
IS1595	1.040e-98	83.24	family transposase n=3 Tax=Roseobacteraceae TaxID=...	[more]
DDE_Tnp_IS1595	4.220e-98	84.92	domain-containing protein n=2 Tax=Rhodobacterales ...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
H-canaliculatus_M_contigs75445	contig	H-canaliculatus_M_contigs75445:3..539 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90	2022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro7m male	2022-07-08

Properties


Property Name	Value
Stop	0
Start	1
Seed ortholog	391593.RCCS2_13849
PFAMs	DDE_Tnp_IS1595,Zn_Tnp_IS1595
Model size	537
Max annot lvl	28211\|Alphaproteobacteria
Hectar predicted targeting category	signal peptide
Exons	1
Evalue	5.44e-99
EggNOG OGs	COG3677@1\|root,COG3677@2\|Bacteria,1MXYX@1224\|Proteobacteria,2TQR3@28211\|Alphaproteobacteria
Description	Transposase and inactivated derivatives
Cds size	519
COG category	L

Relationships

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696854091.5164783-UTR-H-canaliculatus_M_contigs75445:2..20	1696854091.5164783-UTR-H-canaliculatus_M_contigs75445:2..20	Hapterophycus canaliculatus Oshoro7m male	UTR	H-canaliculatus_M_contigs75445 3..20 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696854091.5286553-CDS-H-canaliculatus_M_contigs75445:20..539	1696854091.5286553-CDS-H-canaliculatus_M_contigs75445:20..539	Hapterophycus canaliculatus Oshoro7m male	CDS	H-canaliculatus_M_contigs75445 21..539 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_H-canaliculatus_M_contigs75445.1.1	prot_H-canaliculatus_M_contigs75445.1.1	Hapterophycus canaliculatus Oshoro7m male	polypeptide	H-canaliculatus_M_contigs75445 21..539 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at H-canaliculatus_M_contigs75445:3..539+
coding sequence from alignment at H-canaliculatus_M_contigs75445:3..539+

protein sequence of mRNA_H-canaliculatus_M_contigs75445.1.1

>prot_H-canaliculatus_M_contigs75445.1.1 ID=prot_H-canaliculatus_M_contigs75445.1.1|Name=mRNA_H-canaliculatus_M_contigs75445.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=polypeptide|length=173bp
MDFVDLLAAIFLIVNASKGISMVQLSRDLDCQYKTAFVLAHKLREAMALE
VHTGEVLEGHVEIDGAFFGGHIRPENAKKDRKDRRLKENQTGKRRVVIAM
REREGRTLPFVRKSEGEGVALANENVSRTATMSADEAGHWDLLHMGYDVD
RVNHSEIYSDDGKHTNMVESFFS

mRNA from alignment at H-canaliculatus_M_contigs75445:3..539+

Legend: UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_H-canaliculatus_M_contigs75445.1.1 ID=mRNA_H-canaliculatus_M_contigs75445.1.1|Name=mRNA_H-canaliculatus_M_contigs75445.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=mRNA|length=537bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs75445:3..539+ (Hapterophycus canaliculatus Oshoro7m male)
ATCTTTGCCTCGCGCAAGATGGACTTCGTGGACCTGCTGGCCGCTATCTT
CCTGATCGTGAACGCCTCCAAGGGCATCAGCATGGTGCAGTTGTCGCGTG
ACCTTGATTGTCAGTACAAGACTGCCTTCGTTTTGGCCCACAAATTGCGT
GAGGCGATGGCGTTGGAAGTCCACACGGGCGAAGTGTTGGAAGGCCATGT
CGAGATCGATGGCGCATTCTTCGGTGGTCACATCCGCCCTGAGAACGCCA
AGAAAGACCGCAAGGACCGCCGCCTGAAAGAAAACCAGACGGGCAAGCGC
CGCGTCGTTATCGCCATGCGTGAGCGTGAGGGCCGCACACTGCCATTCGT
GCGCAAGTCTGAGGGCGAAGGCGTTGCGCTGGCAAACGAGAACGTGAGCC
GCACAGCAACCATGTCAGCCGACGAGGCGGGCCACTGGGACTTGCTGCAC
ATGGGTTATGACGTTGACCGCGTGAACCACTCCGAAATCTACAGCGACGA
CGGCAAGCACACAAACATGGTGGAAAGCTTCTTTAGC

Coding sequence (CDS) from alignment at H-canaliculatus_M_contigs75445:3..539+

>mRNA_H-canaliculatus_M_contigs75445.1.1 ID=mRNA_H-canaliculatus_M_contigs75445.1.1|Name=mRNA_H-canaliculatus_M_contigs75445.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=CDS|length=519bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs75445:3..539+ (Hapterophycus canaliculatus Oshoro7m male)
ATGGACTTCGTGGACCTGCTGGCCGCTATCTTCCTGATCGTGAACGCCTC
CAAGGGCATCAGCATGGTGCAGTTGTCGCGTGACCTTGATTGTCAGTACA
AGACTGCCTTCGTTTTGGCCCACAAATTGCGTGAGGCGATGGCGTTGGAA
GTCCACACGGGCGAAGTGTTGGAAGGCCATGTCGAGATCGATGGCGCATT
CTTCGGTGGTCACATCCGCCCTGAGAACGCCAAGAAAGACCGCAAGGACC
GCCGCCTGAAAGAAAACCAGACGGGCAAGCGCCGCGTCGTTATCGCCATG
CGTGAGCGTGAGGGCCGCACACTGCCATTCGTGCGCAAGTCTGAGGGCGA
AGGCGTTGCGCTGGCAAACGAGAACGTGAGCCGCACAGCAACCATGTCAG
CCGACGAGGCGGGCCACTGGGACTTGCTGCACATGGGTTATGACGTTGAC
CGCGTGAACCACTCCGAAATCTACAGCGACGACGGCAAGCACACAAACAT
GGTGGAAAGCTTCTTTAGC