mRNA_H-canaliculatus_M_contigs75122.1.1 (mRNA) Hapterophycus canaliculatus Oshoro7m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_M_contigs75122.1.1
Unique NamemRNA_H-canaliculatus_M_contigs75122.1.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro7m male (Hapterophycus canaliculatus Oshoro7m male)
Homology
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: Phosphoenolpyruvate-protein (phosphotransferase (Fragment) n=1 Tax=Sulfitobacter sp. TaxID=1903071 RepID=A0A3D2F523_9RHOB)

HSP 1 Score: 295 bits (755), Expect = 1.510e-95
Identity = 155/180 (86.11%), Postives = 167/180 (92.78%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIELN 540
            EDI RGLSAEAAVEKEQS+ARARM  V+DSYLRERLSDLDDLSNRMLRILTGQG +TGAEMP DPILVA NIGPAELL+YGR LKGIILE GSVGSHAAIVARALAIPLIVHV RITTEALNGD VM+DGEQGIVHLRPD++VV+AFRDKIAMEAEAQ RYASIRDK A TKCG++IE++
Sbjct:   34 EDIGRGLSAEAAVEKEQSIARARMGQVSDSYLRERLSDLDDLSNRMLRILTGQGQNTGAEMPADPILVASNIGPAELLEYGRSLKGIILEKGSVGSHAAIVARALAIPLIVHVDRITTEALNGDHVMLDGEQGIVHLRPDETVVSAFRDKIAMEAEAQQRYASIRDKPAMTKCGSLIEMH 213
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: Phosphoenolpyruvate--protein (phosphotransferase n=1 Tax=Thalassococcus sp. S3 TaxID=2017482 RepID=A0A4P6MLW5_9RHOB)

HSP 1 Score: 293 bits (749), Expect = 3.590e-92
Identity = 149/180 (82.78%), Postives = 167/180 (92.78%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIELN 540
            EDISRGLSAEAAVEKEQSVARARME V DSYLRERLSDLDDLSNR+LRILTGQG++TGAEMP DPIL+ARNIGP ELL+YGR L+GI+LE GSVGSHAAIVARALAIPL+VH GRITTEALN DQ+MVDG+QGIVHLRPDD+VVTAFRDKIAM+A+A  RYASIRDK A+T CG+++ L+
Sbjct:  268 EDISRGLSAEAAVEKEQSVARARMEQVQDSYLRERLSDLDDLSNRLLRILTGQGSETGAEMPPDPILIARNIGPGELLEYGRKLRGIVLEEGSVGSHAAIVARALAIPLVVHAGRITTEALNNDQIMVDGDQGIVHLRPDDTVVTAFRDKIAMQAQALERYASIRDKHAQTLCGSVVSLH 447
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: Phosphoenolpyruvate--protein (phosphotransferase n=2 Tax=Rhodobacterales TaxID=204455 RepID=A0A8J7JAC8_9RHOB)

HSP 1 Score: 292 bits (748), Expect = 5.050e-92
Identity = 150/180 (83.33%), Postives = 166/180 (92.22%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIELN 540
            EDISRGLSAEAAVEKEQS+ARAR+  VTDSYLRERLSDLDDLSNR+LRILTGQG +TGA++P DPIL+ARNIGPAELL+YGR L+G++LE GSVGSHAAIVARALAIPLIVH GRI TEALNGD +MVDG+QG+VHLRPDDSVVTAFRDKIAM+AEAQ RYASIRD  A T+CGN IELN
Sbjct:  268 EDISRGLSAEAAVEKEQSLARARIGQVTDSYLRERLSDLDDLSNRLLRILTGQGRETGADLPPDPILIARNIGPAELLEYGRSLRGVVLEDGSVGSHAAIVARALAIPLIVHAGRIATEALNGDHIMVDGDQGVVHLRPDDSVVTAFRDKIAMQAEAQQRYASIRDTPAVTRCGNRIELN 447
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: phosphoenolpyruvate--protein (phosphotransferase n=1 Tax=Aestuariivita boseongensis TaxID=1470562 RepID=UPI000683748B)

HSP 1 Score: 291 bits (746), Expect = 9.980e-92
Identity = 149/180 (82.78%), Postives = 167/180 (92.78%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIELN 540
            EDI+RGLSAEAAVEKEQS ARARME V D+YLRERLSDLDDLSNRMLRILTGQGA+TGAEMP DPIL+ARNIGPAELL+YGR LKGI+LE GSVGSHAAIVARALAIPL+VH GRITTEALNGD +MVDG+QG+VHLRPDD+VV+AFR+KIAM+A+AQ RYASI+DK AET CG +I L+
Sbjct:  268 EDIARGLSAEAAVEKEQSTARARMEQVQDAYLRERLSDLDDLSNRMLRILTGQGAETGAEMPDDPILIARNIGPAELLEYGRKLKGIVLEDGSVGSHAAIVARALAIPLVVHAGRITTEALNGDHIMVDGDQGLVHLRPDDTVVSAFREKIAMQAQAQERYASIKDKPAETLCGKVINLH 447
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: phosphoenolpyruvate--protein (phosphotransferase n=1 Tax=Roseobacter sp. YSTF-M11 TaxID=2859783 RepID=UPI001C5E1C2F)

HSP 1 Score: 291 bits (744), Expect = 1.970e-91
Identity = 152/179 (84.92%), Postives = 163/179 (91.06%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIEL 537
            EDIS GLSAEAAVEKEQS+ARARM  VTD+YLRERLSDLDDLSNR+LRILTGQGADTGAEMP DPIL+ARNIGPAELLDYGR LKGIILE GSVGSHAAIVARALAIPL+VH  RITTEALNGD +MVDGEQG+VHLRPDD+VVTAFRDKIAM A AQ RYASIRDK AET+ G ++ L
Sbjct:  268 EDISNGLSAEAAVEKEQSLARARMGKVTDNYLRERLSDLDDLSNRLLRILTGQGADTGAEMPADPILIARNIGPAELLDYGRSLKGIILEQGSVGSHAAIVARALAIPLVVHANRITTEALNGDHIMVDGEQGVVHLRPDDNVVTAFRDKIAMLAAAQERYASIRDKPAETRDGAVLSL 446
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: Phosphoenolpyruvate--protein (phosphotransferase n=3 Tax=Primorskyibacter TaxID=1068904 RepID=A0A1W2CW38_9RHOB)

HSP 1 Score: 289 bits (740), Expect = 7.540e-91
Identity = 147/179 (82.12%), Postives = 164/179 (91.62%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIEL 537
            EDI RGLSAEAAVEKEQS AR+RM  VTD+YLRERLSDLDDLSNR+LR+L+GQG++TGAE+P DPILVARNIGPAELLDYGR LKG++LE GSVGSHAAIVARALAIPL+VH GRITTEALNGD +MVDG+QG+VHLRPDD+VVTAFRDK+AM+AEAQ RYASIRDK AET CG  I L
Sbjct:  268 EDIQRGLSAEAAVEKEQSTARSRMAQVTDNYLRERLSDLDDLSNRLLRLLSGQGSETGAELPDDPILVARNIGPAELLDYGRSLKGVVLEEGSVGSHAAIVARALAIPLVVHAGRITTEALNGDHIMVDGDQGLVHLRPDDTVVTAFRDKMAMQAEAQERYASIRDKPAETLCGKTISL 446
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: Phosphoenolpyruvate--protein (phosphotransferase n=5 Tax=Tateyamaria TaxID=299261 RepID=A0A1P8MVL6_9RHOB)

HSP 1 Score: 289 bits (739), Expect = 1.100e-90
Identity = 152/180 (84.44%), Postives = 163/180 (90.56%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIELN 540
            EDI+ GLSAEAAVEKEQS+ARARM   TD+YLRERLSDLDDLSNR+LRILTGQGADTGAEMP DPILVARNIGPAELLDYGR LKGIILEAGSVGSHAAIVARALAIPLIVH  R T EALNGD VMVDGEQGIVHLRPDD+VVTAFRDK+AM+A A  RYASIRDK AET CG+++ L+
Sbjct:  267 EDIANGLSAEAAVEKEQSLARARMGQATDNYLRERLSDLDDLSNRLLRILTGQGADTGAEMPADPILVARNIGPAELLDYGRSLKGIILEAGSVGSHAAIVARALAIPLIVHAERATLEALNGDHVMVDGEQGIVHLRPDDTVVTAFRDKMAMQAAAVERYASIRDKPAETVCGSVVGLH 446
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: Phosphoenolpyruvate--protein (phosphotransferase n=1 Tax=Ascidiaceihabitans donghaensis TaxID=1510460 RepID=A0A2R8BGC2_9RHOB)

HSP 1 Score: 287 bits (735), Expect = 4.050e-90
Identity = 151/180 (83.89%), Postives = 163/180 (90.56%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIELN 540
            E IS GLSAEAAVEKEQS+ARARME+V D+YLRERLSDLDDLSNRMLRILTGQGADTGAEMP DPILVARNIGPAELL+YGR LKGIILE GSVGSHAAIVARALAIPLIV   R+T EALNGD VMVDGEQGIVHLRPDD+VVTAFRDK+AM+ EA  RYASI+DK A T+CG I+EL+
Sbjct:  266 EAISNGLSAEAAVEKEQSIARARMESVADNYLRERLSDLDDLSNRMLRILTGQGADTGAEMPPDPILVARNIGPAELLEYGRNLKGIILEGGSVGSHAAIVARALAIPLIVQTARVTNEALNGDHVMVDGEQGIVHLRPDDTVVTAFRDKMAMQTEALKRYASIQDKPALTECGQIVELH 445
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: Phosphoenolpyruvate--protein (phosphotransferase n=1 Tax=Roseobacter ponti TaxID=1891787 RepID=A0A858SSL7_9RHOB)

HSP 1 Score: 287 bits (735), Expect = 4.220e-90
Identity = 149/179 (83.24%), Postives = 162/179 (90.50%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIEL 537
            +DI  GLSAEAAVEKEQS+ARARM  VTD+YLRERLSDLDDLSNR+LRILTGQG DTGAEMP +PILVARNIGPAELL+YGR L+GI+LE GSVGSHAAIVARALAIPL+VH  R+TTEALNGDQ+MVDGEQGIVHLRPDD+VVTAFRDKIAM AEAQ RYASIRDK A T CGN + L
Sbjct:  268 DDIGGGLSAEAAVEKEQSLARARMGQVTDNYLRERLSDLDDLSNRLLRILTGQGKDTGAEMPANPILVARNIGPAELLEYGRSLRGIVLEEGSVGSHAAIVARALAIPLVVHASRVTTEALNGDQIMVDGEQGIVHLRPDDTVVTAFRDKIAMLAEAQERYASIRDKPATTACGNTVGL 446
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Match: Phosphoenolpyruvate--protein (phosphotransferase n=1 Tax=Sulfitobacter sabulilitoris TaxID=2562655 RepID=A0A5S3QDF0_9RHOB)

HSP 1 Score: 286 bits (733), Expect = 8.340e-90
Identity = 149/179 (83.24%), Postives = 163/179 (91.06%), Query Frame = 1 
Query:    1 EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRILTGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAIVARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDKIAMEAEAQHRYASIRDKRAETKCGNIIEL 537
            EDISRGLSAEAAVEKEQS+ARARM  V+DSYLRERLSDLDDLSNR+LRILTGQGADTGAEMP DPILVARNIGPAELL+YGR L+GI+LE GSVGSHAAIVARALAIPL+VH GRIT EALNGD +MVDGEQG+VHLRPDD+V +AFRDKIAM A AQ RY SIRDK A+T CG++I L
Sbjct:  268 EDISRGLSAEAAVEKEQSLARARMGQVSDSYLRERLSDLDDLSNRLLRILTGQGADTGAEMPPDPILVARNIGPAELLEYGRSLRGIVLEEGSVGSHAAIVARALAIPLVVHAGRITNEALNGDYIMVDGEQGLVHLRPDDTVSSAFRDKIAMLAAAQERYTSIRDKPAKTLCGSVISL 446
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_M_contigs75122.1.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Phosphoenolpyruvate-protein1.510e-9586.11phosphotransferase (Fragment) n=1 Tax=Sulfitobacte... [more]
Phosphoenolpyruvate--protein3.590e-9282.78phosphotransferase n=1 Tax=Thalassococcus sp. S3 T... [more]
Phosphoenolpyruvate--protein5.050e-9283.33phosphotransferase n=2 Tax=Rhodobacterales TaxID=2... [more]
phosphoenolpyruvate--protein9.980e-9282.78phosphotransferase n=1 Tax=Aestuariivita boseongen... [more]
phosphoenolpyruvate--protein1.970e-9184.92phosphotransferase n=1 Tax=Roseobacter sp. YSTF-M1... [more]
Phosphoenolpyruvate--protein7.540e-9182.12phosphotransferase n=3 Tax=Primorskyibacter TaxID=... [more]
Phosphoenolpyruvate--protein1.100e-9084.44phosphotransferase n=5 Tax=Tateyamaria TaxID=29926... [more]
Phosphoenolpyruvate--protein4.050e-9083.89phosphotransferase n=1 Tax=Ascidiaceihabitans dong... [more]
Phosphoenolpyruvate--protein4.220e-9083.24phosphotransferase n=1 Tax=Roseobacter ponti TaxID... [more]
Phosphoenolpyruvate--protein8.340e-9083.24phosphotransferase n=1 Tax=Sulfitobacter sabulilit... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_M_contigs75122contigH-canaliculatus_M_contigs75122:3..542 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro7m male2022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog391624.OIHEL45_09858
Preferred nameptsP
PFAMsGAF,GAF_2,PEP-utilisers_N,PEP-utilizers,PEP-utilizers_C
Model size540
Max annot lvl28211|Alphaproteobacteria
KEGG koko:K08484
KEGG Pathwayko02060,map02060
Hectar predicted targeting categoryother localisation
Exons1
Evalue4.45e-92
EggNOG OGsCOG3605@1|root,COG3605@2|Bacteria,1QTTV@1224|Proteobacteria,2TR3Z@28211|Alphaproteobacteria
EC2.7.3.9
DescriptionBelongs to the PEP-utilizing enzyme family
Cds size540
COG categoryT
BRITEko00000,ko00001,ko01000,ko02000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696854089.091883-CDS-H-canaliculatus_M_contigs75122:2..5421696854089.091883-CDS-H-canaliculatus_M_contigs75122:2..542Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs75122 3..542 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_M_contigs75122.1.1prot_H-canaliculatus_M_contigs75122.1.1Hapterophycus canaliculatus Oshoro7m malepolypeptideH-canaliculatus_M_contigs75122 3..542 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_M_contigs75122.1.1

>prot_H-canaliculatus_M_contigs75122.1.1 ID=prot_H-canaliculatus_M_contigs75122.1.1|Name=mRNA_H-canaliculatus_M_contigs75122.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=polypeptide|length=180bp
EDISRGLSAEAAVEKEQSVARARMETVTDSYLRERLSDLDDLSNRMLRIL
TGQGADTGAEMPVDPILVARNIGPAELLDYGRGLKGIILEAGSVGSHAAI
VARALAIPLIVHVGRITTEALNGDQVMVDGEQGIVHLRPDDSVVTAFRDK
IAMEAEAQHRYASIRDKRAETKCGNIIELN
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mRNA from alignment at H-canaliculatus_M_contigs75122:3..542+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_M_contigs75122.1.1 ID=mRNA_H-canaliculatus_M_contigs75122.1.1|Name=mRNA_H-canaliculatus_M_contigs75122.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=mRNA|length=540bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs75122:3..542+ (Hapterophycus canaliculatus Oshoro7m male)
GAAGACATCAGCCGTGGTCTAAGTGCCGAGGCGGCCGTCGAAAAAGAACA
ATCTGTCGCGCGTGCGCGGATGGAAACGGTCACGGACAGCTACCTGCGCG
AACGGCTGAGCGATCTGGATGATCTGTCAAACCGGATGCTGCGGATTTTG
ACCGGGCAGGGTGCTGATACGGGGGCCGAAATGCCGGTCGATCCGATTTT
GGTTGCGCGCAACATTGGTCCTGCGGAATTGCTGGATTACGGGCGCGGGC
TCAAGGGAATCATCCTTGAGGCAGGGTCGGTCGGCAGCCACGCCGCCATC
GTCGCCCGTGCCCTTGCGATCCCGCTGATTGTGCACGTGGGGCGCATCAC
CACCGAGGCGCTGAACGGCGATCAGGTGATGGTCGACGGCGAACAAGGCA
TCGTGCATCTGCGCCCCGATGACAGTGTCGTCACGGCGTTTCGCGACAAA
ATCGCAATGGAGGCCGAGGCGCAGCATCGCTATGCGTCGATCCGTGACAA
GCGCGCAGAAACCAAATGCGGCAACATTATTGAGCTGAAC
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Coding sequence (CDS) from alignment at H-canaliculatus_M_contigs75122:3..542+

>mRNA_H-canaliculatus_M_contigs75122.1.1 ID=mRNA_H-canaliculatus_M_contigs75122.1.1|Name=mRNA_H-canaliculatus_M_contigs75122.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=CDS|length=540bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs75122:3..542+ (Hapterophycus canaliculatus Oshoro7m male)
GAAGACATCAGCCGTGGTCTAAGTGCCGAGGCGGCCGTCGAAAAAGAACA
ATCTGTCGCGCGTGCGCGGATGGAAACGGTCACGGACAGCTACCTGCGCG
AACGGCTGAGCGATCTGGATGATCTGTCAAACCGGATGCTGCGGATTTTG
ACCGGGCAGGGTGCTGATACGGGGGCCGAAATGCCGGTCGATCCGATTTT
GGTTGCGCGCAACATTGGTCCTGCGGAATTGCTGGATTACGGGCGCGGGC
TCAAGGGAATCATCCTTGAGGCAGGGTCGGTCGGCAGCCACGCCGCCATC
GTCGCCCGTGCCCTTGCGATCCCGCTGATTGTGCACGTGGGGCGCATCAC
CACCGAGGCGCTGAACGGCGATCAGGTGATGGTCGACGGCGAACAAGGCA
TCGTGCATCTGCGCCCCGATGACAGTGTCGTCACGGCGTTTCGCGACAAA
ATCGCAATGGAGGCCGAGGCGCAGCATCGCTATGCGTCGATCCGTGACAA
GCGCGCAGAAACCAAATGCGGCAACATTATTGAGCTGAAC
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