mRNA_H-canaliculatus_M_contigs74572.1.1 (mRNA) Hapterophycus canaliculatus Oshoro7m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_M_contigs74572.1.1
Unique NamemRNA_H-canaliculatus_M_contigs74572.1.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro7m male (Hapterophycus canaliculatus Oshoro7m male)
Homology
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BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoylalanine--D-glutamate (ligase n=3 Tax=unclassified Jannaschia TaxID=2645469 RepID=MURD_JANSC)

HSP 1 Score: 179 bits (453), Expect = 3.370e-52
Identity = 92/106 (86.79%), Postives = 98/106 (92.45%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEALR 318
            ALGAFKRIRWICGGLEKDGG+E L  AAG VAKAYVIGREAEAFALHLG  P D+CGDMATAVARA+ADA+PGDTVLLAPAAASFDQYDSFETRGADFIA+VE L+
Sbjct:  354 ALGAFKRIRWICGGLEKDGGLEALWEAAGHVAKAYVIGREAEAFALHLGDIPKDVCGDMATAVARASADAQPGDTVLLAPAAASFDQYDSFETRGADFIAQVEKLQ 459
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BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoylalanine--D-glutamate (ligase n=1 Tax=Gymnodinialimonas ceratoperidinii TaxID=2856823 RepID=A0A8F6YCH8_9RHOB)

HSP 1 Score: 173 bits (438), Expect = 6.040e-50
Identity = 88/107 (82.24%), Postives = 98/107 (91.59%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEALRG 321
            AL AF++IRWICGGLEKDGG+E L  AAG V KAYVIGREAEAFALHLG  P++ICGDMATAVARA+A+A+PGDTVLLAPAAASFDQYDSFE RGADFIA+VEAL+G
Sbjct:  358 ALDAFRKIRWICGGLEKDGGLEPLHGAAGEVLKAYVIGREAEAFALHLGDIPSEICGDMATAVARASAEAQPGDTVLLAPAAASFDQYDSFEARGADFIAQVEALQG 464
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BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoyl-L-alanine--D-glutamate (ligase n=1 Tax=Hasllibacter sp. MH4015 TaxID=2854029 RepID=UPI001CD51D8C)

HSP 1 Score: 172 bits (435), Expect = 1.710e-49
Identity = 90/106 (84.91%), Postives = 94/106 (88.68%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEALR 318
            ALGAFKRIRWICGGLEKDGG   L  AAG V KAYVIGREAEAFALHLG  P +ICG+M TAVARAAA+AEPGDTVLLAPAAASFDQYDSFE RGADFIA+VEALR
Sbjct:  359 ALGAFKRIRWICGGLEKDGGFGGLQDAAGGVLKAYVIGREAEAFALHLGDVPVEICGNMETAVARAAAEAEPGDTVLLAPAAASFDQYDSFEARGADFIAQVEALR 464
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BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoylalanine--D-glutamate (ligase n=1 Tax=Rhodobacteraceae bacterium HLUCCO18 TaxID=1666917 RepID=A0A0P8APG6_9RHOB)

HSP 1 Score: 161 bits (407), Expect = 2.280e-45
Identity = 85/107 (79.44%), Postives = 92/107 (85.98%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEALRG 321
            AL AF RIRWICGGL K+GGME LAAAAG VAKAYVIGREAE FAL LG  P ++CG+MATAVARAAA+AEPGDTVLLAPAAASFDQYD+FE RG DFIA+V  L G
Sbjct:  359 ALSAFPRIRWICGGLAKEGGMEALAAAAGNVAKAYVIGREAEGFALGLGDLPHEVCGEMATAVARAAAEAEPGDTVLLAPAAASFDQYDNFEKRGDDFIAQVGKLSG 465
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BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoylalanine--D-glutamate (ligase n=1 Tax=Roseibacterium persicicum TaxID=2650661 RepID=A0A7X6GYI9_9RHOB)

HSP 1 Score: 159 bits (403), Expect = 8.840e-45
Identity = 84/107 (78.50%), Postives = 91/107 (85.05%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEALRG 321
            ALGAF RIRWICGGLEK+GG+  LA AAG V KAYVIGREA AFAL LG  P +ICGDMATAVARAAA+A+PGDTVLLAPAAASFDQYDSFE RG DF+ +V  LRG
Sbjct:  359 ALGAFTRIRWICGGLEKEGGLAALAPAAGEVVKAYVIGREAAAFALKLGAIPHEICGDMATAVARAAAEAQPGDTVLLAPAAASFDQYDSFEHRGRDFMEQVGKLRG 465
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BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoylalanine--D-glutamate (ligase n=1 Tax=Roseicyclus mahoneyensis TaxID=164332 RepID=A0A316GJY2_9RHOB)

HSP 1 Score: 157 bits (397), Expect = 6.600e-44
Identity = 83/105 (79.05%), Postives = 89/105 (84.76%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEAL 315
            AL AF+RIRWICGGLEK+GGM  LA AAG V KAYVIGREA AFALHLG    +ICGDMATAVARAAA+AEPGDTVLLAPAAASFDQYDSFE RG DF+ +V  L
Sbjct:  359 ALAAFRRIRWICGGLEKEGGMAALAPAAGEVVKAYVIGREAAAFALHLGAVRHEICGDMATAVARAAAEAEPGDTVLLAPAAASFDQYDSFEHRGRDFMEQVAKL 463
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BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoyl-L-alanine--D-glutamate (ligase n=1 Tax=Maritalea mobilis TaxID=483324 RepID=UPI001C96A82A)

HSP 1 Score: 156 bits (394), Expect = 1.820e-43
Identity = 79/105 (75.24%), Postives = 89/105 (84.76%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEAL 315
            AL AF RIRWICGGLEK+GGM+ LA A G V KAYVIGREA  FALHLG  P ++CGDMATAV+RAAA+AEPG+TVLLAPAAASFDQYD+FE RG DF+A+V  L
Sbjct:  359 ALSAFPRIRWICGGLEKEGGMQALAGATGNVVKAYVIGREAAGFALHLGDLPHEVCGDMATAVSRAAAEAEPGETVLLAPAAASFDQYDNFEQRGDDFVAQVAKL 463
[Close]
BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoylalanine--D-glutamate (ligase n=1 Tax=Roseibacterium elongatum DSM 19469 TaxID=1294273 RepID=W8S630_9RHOB)

HSP 1 Score: 150 bits (380), Expect = 1.030e-42
Identity = 77/105 (73.33%), Postives = 86/105 (81.90%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEAL 315
            AL AF RIRWICGGLEKDGGM+ L+ A   V KAYVIGREA  FALHLG  P ++CGDM TAVARAA++AEPG+TVLLAPAAASFDQYD+FE RG DF A+V  L
Sbjct:  209 ALSAFPRIRWICGGLEKDGGMDALSGAMDHVVKAYVIGREAAGFALHLGDLPHEVCGDMVTAVARAASEAEPGETVLLAPAAASFDQYDNFERRGEDFAAQVARL 313
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BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoylalanine--D-glutamate (ligase n=2 Tax=Pseudooceanicola marinus TaxID=396013 RepID=A0A1X6ZG70_9RHOB)

HSP 1 Score: 149 bits (377), Expect = 5.880e-41
Identity = 79/107 (73.83%), Postives = 88/107 (82.24%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEALRG 321
            AL AFKRIRWICGGL+K+GG+E L  AAG V KAYVIGREAE FA+ L     ++C DMATAVARAAA+A+PG+TVLLAPAAASFDQYDSFE RG DF ARV AL G
Sbjct:  360 ALSAFKRIRWICGGLQKEGGLEPLLEAAGEVVKAYVIGREAEDFAMQLKGIEAEVCTDMATAVARAAAEAQPGETVLLAPAAASFDQYDSFEKRGEDFAARVIALSG 466
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BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Match: UDP-N-acetylmuramoylalanine--D-glutamate (ligase n=1 Tax=Litoreibacter ponti TaxID=1510457 RepID=A0A2T6BDB3_9RHOB)

HSP 1 Score: 149 bits (376), Expect = 7.840e-41
Identity = 78/105 (74.29%), Postives = 84/105 (80.00%), Query Frame = 1 
Query:    1 ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQTPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIARVEAL 315
            AL AF+ IRWICGGLEKDGG+E L    G V KAYVIGREA +FA+ LG  P ++C DM TAVARAA DAEPGDTVLLAPAAASFDQYDSFE RG DFIA VE L
Sbjct:  360 ALQAFRNIRWICGGLEKDGGLEALLPHLGGVTKAYVIGREAPSFAMKLGDLPHEVCSDMVTAVARAAEDAEPGDTVLLAPAAASFDQYDSFERRGEDFIACVERL 464
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_M_contigs74572.1.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UDP-N-acetylmuramoylalanine--D-glutamate3.370e-5286.79ligase n=3 Tax=unclassified Jannaschia TaxID=26454... [more]
UDP-N-acetylmuramoylalanine--D-glutamate6.040e-5082.24ligase n=1 Tax=Gymnodinialimonas ceratoperidinii T... [more]
UDP-N-acetylmuramoyl-L-alanine--D-glutamate1.710e-4984.91ligase n=1 Tax=Hasllibacter sp. MH4015 TaxID=28540... [more]
UDP-N-acetylmuramoylalanine--D-glutamate2.280e-4579.44ligase n=1 Tax=Rhodobacteraceae bacterium HLUCCO18... [more]
UDP-N-acetylmuramoylalanine--D-glutamate8.840e-4578.50ligase n=1 Tax=Roseibacterium persicicum TaxID=265... [more]
UDP-N-acetylmuramoylalanine--D-glutamate6.600e-4479.05ligase n=1 Tax=Roseicyclus mahoneyensis TaxID=1643... [more]
UDP-N-acetylmuramoyl-L-alanine--D-glutamate1.820e-4375.24ligase n=1 Tax=Maritalea mobilis TaxID=483324 RepI... [more]
UDP-N-acetylmuramoylalanine--D-glutamate1.030e-4273.33ligase n=1 Tax=Roseibacterium elongatum DSM 19469 ... [more]
UDP-N-acetylmuramoylalanine--D-glutamate5.880e-4173.83ligase n=2 Tax=Pseudooceanicola marinus TaxID=3960... [more]
UDP-N-acetylmuramoylalanine--D-glutamate7.840e-4174.29ligase n=1 Tax=Litoreibacter ponti TaxID=1510457 R... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_M_contigs74572contigH-canaliculatus_M_contigs74572:2..325 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro7m male vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro7m male2022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog290400.Jann_2763
Preferred namemurD
PFAMsMur_ligase_C,Mur_ligase_M
Model size324
Max annot lvl28211|Alphaproteobacteria
KEGG rclassRC00064,RC00141
KEGG koko:K01925
KEGG ReactionR02783
KEGG Pathwayko00471,ko00550,ko01100,map00471,map00550,map01100
Hectar predicted targeting categoryother localisation
Exons1
Evalue5.52e-53
EggNOG OGsCOG0771@1|root,COG0771@2|Bacteria,1MVYD@1224|Proteobacteria,2TTE8@28211|Alphaproteobacteria
EC6.3.2.9
DescriptionCell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
Cds size324
COG categoryM
BRITEko00000,ko00001,ko01000,ko01011
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696854083.1936214-CDS-H-canaliculatus_M_contigs74572:1..3251696854083.1936214-CDS-H-canaliculatus_M_contigs74572:1..325Hapterophycus canaliculatus Oshoro7m maleCDSH-canaliculatus_M_contigs74572 2..325 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_M_contigs74572.1.1prot_H-canaliculatus_M_contigs74572.1.1Hapterophycus canaliculatus Oshoro7m malepolypeptideH-canaliculatus_M_contigs74572 2..325 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_M_contigs74572.1.1

>prot_H-canaliculatus_M_contigs74572.1.1 ID=prot_H-canaliculatus_M_contigs74572.1.1|Name=mRNA_H-canaliculatus_M_contigs74572.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=polypeptide|length=108bp
ALGAFKRIRWICGGLEKDGGMETLAAAAGAVAKAYVIGREAEAFALHLGQ
TPTDICGDMATAVARAAADAEPGDTVLLAPAAASFDQYDSFETRGADFIA
RVEALRG*
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mRNA from alignment at H-canaliculatus_M_contigs74572:2..325+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_M_contigs74572.1.1 ID=mRNA_H-canaliculatus_M_contigs74572.1.1|Name=mRNA_H-canaliculatus_M_contigs74572.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=mRNA|length=324bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs74572:2..325+ (Hapterophycus canaliculatus Oshoro7m male)
GCCTTGGGCGCGTTCAAGCGCATCCGCTGGATTTGCGGTGGGCTGGAGAA
GGACGGCGGGATGGAGACCTTGGCCGCGGCAGCTGGCGCGGTTGCCAAAG
CCTATGTGATCGGGCGCGAGGCAGAGGCCTTTGCCCTGCACTTGGGCCAA
ACCCCCACGGACATCTGCGGCGACATGGCAACCGCCGTGGCACGGGCAGC
GGCTGACGCAGAGCCCGGCGATACCGTGCTTCTTGCGCCAGCGGCCGCCA
GTTTCGACCAATACGACAGCTTCGAGACGCGGGGCGCGGACTTCATCGCA
CGGGTTGAGGCGCTGCGGGGCTAG
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Coding sequence (CDS) from alignment at H-canaliculatus_M_contigs74572:2..325+

>mRNA_H-canaliculatus_M_contigs74572.1.1 ID=mRNA_H-canaliculatus_M_contigs74572.1.1|Name=mRNA_H-canaliculatus_M_contigs74572.1.1|organism=Hapterophycus canaliculatus Oshoro7m male|type=CDS|length=324bp|location=Sequence derived from alignment at H-canaliculatus_M_contigs74572:2..325+ (Hapterophycus canaliculatus Oshoro7m male)
GCCTTGGGCGCGTTCAAGCGCATCCGCTGGATTTGCGGTGGGCTGGAGAA
GGACGGCGGGATGGAGACCTTGGCCGCGGCAGCTGGCGCGGTTGCCAAAG
CCTATGTGATCGGGCGCGAGGCAGAGGCCTTTGCCCTGCACTTGGGCCAA
ACCCCCACGGACATCTGCGGCGACATGGCAACCGCCGTGGCACGGGCAGC
GGCTGACGCAGAGCCCGGCGATACCGTGCTTCTTGCGCCAGCGGCCGCCA
GTTTCGACCAATACGACAGCTTCGAGACGCGGGGCGCGGACTTCATCGCA
CGGGTTGAGGCGCTGCGGGGCTAG
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