mRNA_H-canaliculatus_F_contig117.1509.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 1166 bits (3016), Expect = 0.000e+0
Identity = 603/963 (62.62%), Postives = 726/963 (75.39%), Query Frame = 2

Query: 2024 SVFVTLAALWG----VAADSFPYPSLRAVENTLLTTAKDDFAGQRKGFENAVRWDGERIRAGIRFNDSDQGDVLAPHFACAGYGHGREAFLRLQQLLSPEAVRPASHSSEHGACFFATASHTQVAAIISEHDEFGLESLAPFPSALKLAPGLIEHEANDG--SEPINLSSKLTAKHGGAMRLGNVEGLSVEFSPGTLPAGSSEAELLIKDLLDDLVSESMDLHTTNFWSDPAMLG-EHLVTPGGAIRRRDWSKAATMVHELSKSEHTSPANICSWHHVHAIHVASDFVLISGLNHLLVFGGGPDGG-KNQPTELDVACFMGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFIDGDGMEVQHGYYFDELGLLQGGSSTGSSLFGFPEFHGGDFFYDSDRRKIIQYIDMVKSDSIQGSFGSSFTVSGIAFPWIPADDFGEDDEAGHGTHTAGTAAGATLNNPAETVTCEGGQTLGCAGACIDATGSDDDLVTSFYQRY--DVDRQCPIFGCDDDTEACLGDAVGTTLAENGGMAQGAKLSIFDVFLGELGLTSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDGENPENSVCTIGSPAIAKNVLAVGATSSGETR-FNTDGVYDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGTDDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDDGFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWSSGEKDNVNVNERVVVAAEDVESGTWTVSVSANSLEITDRQSYSLVVNGALI-----GANVMVDNSSP 4864
            +V V   A W      AAD++  PSL  VEN L TTAKDDF G  + FE A  W+GER+R         Q    APH  CA YGHGRE F RL+Q LSP+AVR  SHS +HGACF ATASH Q +AI+ + ++FGLES +PFPSALKLAPGL++H  +     EP +   +L A+HGG MR+ NVEGL+V+ +P TLPA S+ AE  I DLL DL+S S+DLH+TNFWSDP M G EHL + GGA+R RDWSKAA++VHELS++  TSP +ICSW  V  I    D +L++GL+HLL  G G  G  + + TEL VACFMG++SF A RPEVLRVSP H+KR L+  AR IIQ+A++T+TPLT AGLDGTGE+IQVVD+GLDETSCFFID  G EV HGY+F+EL +   GS+  S L  +P F  GDF Y  +RRK+IQYI++V  DS   +F    T  G  +P++P D+FG+DD AGHGTHTAG+AAGATLN+PAET+TC G +TLGC G CID + + DDLV+   Q    D+DR CP++GCD  T+ CL D V  TL +NGGMAQGAKLSIFDVF    GL     NG+WEPCLEAGCK+HS S GG   C  G +++ YD+FMYENPENLLIFAAGNDGE  E SVCT+ SPAI KNVLAVGA++SG+TR  +T+G   ID VA FSS+GFTTDGRIKPEVVAPGDM+YSAASDGTD HSCRLWAY+GTSMS P+VAGA+AM+RQYFVD  FY ADVT+RGFC DGF+CEGFSPS+ATVKALLINSANLMG +S+PD YRGFGRIHLEQGMPL+GEGDLALFVAD+  TSI+E ++  YLFDVDADA ++ RATLSWIDYPAT+ S +QL++DLDL V+SPSGT Y MW S   DN NVNERV+V A+DVE+GTWTV V +NSL +TD QSYSLVVNGA+      GA+   D+S+P
Sbjct:    9 AVVVMACAFWAPARAAAADTWSPPSLELVENGLATTAKDDFNGHARAFEAAATWNGERLRR-------SQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFV---GSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIELVNPDSDYVAFS---TTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGET-ERSVCTMASPAIGKNVLAVGASTSGDTRVISTEGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSL-VTDSQSYSLVVNGAISAGTGEGASGSFDSSNP 956          

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 1065 bits (2755), Expect = 0.000e+0
Identity = 574/987 (58.16%), Postives = 698/987 (70.72%), Query Frame = 2

Query: 2054 GVAADSFPYPSLRAVENTLLTTAKDDFAGQRKGFENAVRWDGERIRAGIRFNDSDQGDVLAPHFACAGYGHGREAFLRLQQLLSPEAVRPASHSSEHGACFFATASHTQVAAIISEHDEFGLESLAPFPSALKLAPGLIEHEANDGSEPINLSS--KLTAKHGGAMRLGNVEGLSVEFSPGTLPAGSSEAELLIKDLLDDLVSESMDLHTTNFWSDPAMLG-EHLVTPGGAIRRRDWSKAATMVHELSKSEHTSPANICSWHHVHAIHVASDFVLISGLNHLLVFGGGPDGGKN--QPTELDVACFMGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFI-DGDGMEVQHGYYFDELGL--------LQGGSSTGSSLFGFP----------------EFHGGDFFYDSDRRKIIQYIDMVKSDSIQGSFGSSFTVS-GIAFPWIPADDFGEDDEAGHGTHTAGTAAGATLNNPAETVTCEGGQTLGCAGACIDATGSDD--DLVTSFYQRYDVDRQCPIFG---CDD-DTEACLGDAVGTTLAENGGMAQGAKLSIFDVFLGELGLTSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDGENPENSVCTIGSPAIAKNVLAVGATSSGETRFNTDGVYDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGT-DDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDDGFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWSSGEKDNVNVNERVVVAAEDVESGTWTVSVSANSLEITDRQSYSLVVNGAL-----IGANVMVDNSSPASSVVSN 4885
            G    S PYPSL  VEN L TTAKDDF G  + FE A  W+GER+R         Q +  APH  CA YGHGRE F RL+Q LSPEAVR  SHS + GACFFA+ASH Q +AII + ++FGLES +PFPSALKLAPGL++H      +    +   +L+A+HG  MR+ NVEGLS+E +PGTL + S EAE  I D L DL+  S+DLH+TNFWSD  M G E L  PGGA+R RDWSKAA++VHELS++  TSP +ICSW+ V   H   D +L+SGL+HLL  G G  G  +  +  EL +ACFMG++SFLAS+PEVLRV+P  S+  L+A+ARA IQ+AT T+TPLT AGLDGTG+++QV+DSG+DE+SCFF  D  G  V HG+YF+E G+            +S      G+P                EF GGDF    DRRKIIQYI+++KSD  +GS G SFT S G  +PW+PAD F  D EAGHGTH AG+ AGATLN PAE ++C+  +T+ C GAC+D   ++D  DL T   +  D+DR CP+F    CD  +TE CL D V  TL  NGGMAQGAKL+IFD F    GL+ F  NGLWEPC EA CKIHSNS+GG   C+LG ND+ YD +M  NPENLLIFAAGNDGE    S CT+ SPAIAKNVLAVGA++SG+TR +      ID VA FSS+G T+D RIKPEV+APGD IYSAASDG+ D HSCRLWAY GTSMSTP+VAGASAMIRQYF++  F+A D+T+RGFCD+   CE FSPSSAT+KAL+INSANLMGG+SEPDG RGFGRIHLE GMPL GEG LALFV+DS  TSI E +   Y FDV+A A +D R TLSW+D PATTFS VQL+HDLDL V+SP+GT YTMW+SG  D+ NVNERV+V A DVE+GTW+V + A++L  TD QSYSLVVNGA+     +GA   ++ SS  SS  S+
Sbjct:   13 GWPGKSLPYPSLELVENALSTTAKDDFNGHARAFEAAATWNGERLR-------HRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGER-RRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLT-TDVQSYSLVVNGAISPGTGVGA---IERSSSTSSTSSD 987          

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 1061 bits (2743), Expect = 0.000e+0
Identity = 567/935 (60.64%), Postives = 670/935 (71.66%), Query Frame = 2

Query: 2057 VAADSFPYPSLRAVENTLLTTAKDDFAGQRKGFENAVRWDGERIRAGIRFNDSDQGDVLAPHFACAGYGHGREAFLRLQQLLSPEAVRPASHSSEHGACFFATASHTQVAAIISEHDEFGLESLAPFPSALKLAPGLIEH-EANDGSEPINLSS---KLTAKHGGAMRLGNVEGLSVEFSPGTLPAGSSEAELLIKDLLDDLVSESMDLHTTNFWSDPAM--LGEHLVTPGGAIRRRDWSKAATMVHELSKSEHTSPANICSWHHVHAIHVASDFVLISGLNHLLVFGGGPDGG-KNQPTELDVACFMGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFIDGDGMEVQHGYYFDELGLLQGGSSTGSSLFGFPEFHGGDFFYDSDRRKIIQYIDMVKSDSIQGSFGSSFTVSGIAFPWIPADDFGEDDEAGHGTHTAGTAAGATLNNPAETVTCEGGQTLGCAGACIDATGSDDDLVTSFYQR--YDVDRQCPIFGCDDDTEA-CLGDAVGTTLAENGGMAQGAKLSIFDVFLGELGLTSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDGENPENSVCTIGSPAIAKNVLAVGATSSGETR-FNTDGVYDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGTDDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDDGFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWSSGEKDNVNVNERVVVAAEDVESGTWTVSVSANSLEITDRQSYSLVVNGAL 4828
            VAA     P+L  VEN LLTTAKDDF G    FE A  W+GER+R         Q +  APH ACA YG G EA  RL+Q LSPEAVR  SHS +HGACFFA+ASH Q AAI+ + ++FGLE+ +PFPS LKLAPGL++H E++D +E    SS   +L+A+HG  MR  NVEGLSVE +PGTL A SSEA+  I  +L DL+S S+DLH+TNFWSDP M   GEHL +P GA+R RDW KAA++VHELS++  T P +ICSW+ +   H   D +L+SGL+HLL  G G  G  + + TEL VACFMG++SFLA RPEVLRVSP  +KR L+A AR +IQ+A++T+TPLT AGLDGTGEIIQV+DSGLDETSC+FID  G EV HGYYFDELG     SS+ SS      F+GGDF Y  +RRK                                         AGHGTHTAG+AAGATLN+PAET+TC    TL C GACID   + DDLV+   Q    D+DR CP++GCD+ T+  CL D V  TL+ NGGMAQGAKL+IFDVF G    +S   N LWE C EAGCK+HSNS G    C++ + D+  D FMYEN ENLLIFAAGN+G   E S CTI +P I KN LA+GA++SG+TR  +T G   ID VA FSSWGFTTD RIKPEVVAPGD +YSAASDGTD HSCRLWAYEGTSMS P+VAGA+AMIRQYFVD  FY ADVT+RGFC DGF+CEGFSPS+ATVKALLINSANLMGG+SEPDG+RGFGR+HLE GMPL+GEGDL LFVAD+  TSI+  +L  YLFDVDADA +D RATLSWID PAT  S VQL+H+LDL V+SP GT+Y MW S   DN NVNERV+V A DV +GTWTV V AN+L +TD QSYSLVVNGA+
Sbjct:   19 VAAGRLNSPALELVENALLTTAKDDFKGHVGAFEAAATWNGERLR-------HSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAVYSSSYSSDETATIFNGGDFSYYPERRK-----------------------------------------AGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEG-GIERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNL-VTDSQSYSLVVNGAI 903          

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 952 bits (2460), Expect = 0.000e+0
Identity = 535/995 (53.77%), Postives = 666/995 (66.93%), Query Frame = 2

Query: 2012 RATFSVFVTLAAL---WGVAADSFPYPSLRAVENTLLTTAKDDFAGQRKGFENAVRWDGERIRAGIRFNDSDQGDVLAPHFACAGYGHGREAFLRLQQLLSPEAVRPASHSSEHGACFFATASHTQVAAIISEHDEFGLESLAPFPSALKLAPGLIEHEANDGSEPINLSSKLTAKHGGAMRLGNVEGLSVEFSPGTLPAGSSEAELLIKDLLDDLVSESMDLHTTNFWSDP-AMLGEHLVTPGGAIRRRDWSKAATMVHELSKSEHTSPANICSWHHVHAIHVASDFVLISGLNHLLVFGGGPDGGKNQPTELDVACFMGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFIDGDGMEVQHGYYFDELGLLQGGSSTGSSLFGFPEFHGGDFFYDSDRRKIIQYIDMVKSDSIQGSFGSSFTVSGIAFPWIPADDFGEDDEAGHGTHTAGTAAGATLNNPAET-VTCEGGQTLGCAGACIDA--TGSDDDLVTSFYQRY----DVDRQCPIFGCDDDTEA-CLGDAVGTTLAENGGMAQGAKLSIFDVFLGELGLTSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDGENPEN-SVCTIGSPAIAKNVLAVGATSSGETRFN-----------TDGVYDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGTDDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDD-GFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWS-SGEKDNVNVNERVVVAAEDVESGTWTVSVSANSLEITDRQSYSLVVNGALIGANVMVDNSSPASSVVSNAWMVLSVVVSI 4918
            R   ++F++L A    WG +A+S PYPSL  VE +LLTTA + F G  KGFE A +W G+ IR+       ++ +  APH  C  Y  G E   RLQ+LLSPEAVR  +HSS HGACFF TASH +  A+ S++  +GL S++PFPSALK+APG++EH  +   +      +L   HG  MR GNVEGL++E SPGTLPA S +A + I +LLDDL+S+S+DLH  N WSDP A  GEHL TP GA+R+R+WS AAT+VH+L+ +  TSP +ICSW  +   H  +D +L+SGL+HLL    G  G   + TEL VACFMG++SFL+SR EV RVSP H+ R L+A AR IIQ+AT T+ PLTAAGLDGTG++IQV                                                              IQYI+++K+DS+ G   SS T  G    W+  D F  DD   HGTHTAG+A GAT+  PAET  TC G +  GC G CIDA  T   DDL+T   +      D+DR CP+ GCD++ E  CL D V  TLA++GGMAQGAKLS FD++ G +    F  NGLWEPCLEAGCKIHS S+G    CA+   DL YD+FMY NPENL++ AAGN GE  +  +VCT+ SPAI KN LAVGATSSGETR             T+G  D+D VA FSS+G T DGRIKPEVVAPGD +YSA SDG+D HSCRL A  GTSM+ P+VAGASAM+RQYF+D  FY AD  +RG+CDD G  CE FSPSSATVKALLINSANLMGG+SEPDG+RGFGRIHLE GMPLDGEG LALFVADS+ TS+ E S  +++FDVDA+A++DFRATLSWID PA++ SGVQL+HDLDL V+SPSG ++ MW+ SGE D+ NVNERV++AAE+VESGT+TV VS N L +TD Q+YSLVVNGA      MV +SS AS+ V ++  +++  VS+
Sbjct:    6 RTATALFLSLVAASTPWGGSANSLPYPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRS------KEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAM-SDNPYYGLTSISPFPSALKVAPGVLEHGVSR-DQASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQV--------------------------------------------------------------IQYINLIKADSVSGP-ASSTTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHL-LTDSQNYSLVVNGA------MVYSSSLASASVQSSGSMMTTPVSL 922          

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 951 bits (2458), Expect = 0.000e+0
Identity = 520/941 (55.26%), Postives = 637/941 (67.69%), Query Frame = 2

Query: 2012 RATFSVFVTLAAL---WGVAADSFPYPSLRAVENTLLTTAKDDFAGQRKGFENAVRWDGERIRAGIRFNDSDQGDVLAPHFACAGYGHGREAFLRLQQLLSPEAVRPASHSSEHGACFFATASHTQVAAIISEHDEFGLESLAPFPSALKLAPGLIEHEANDGSEPINLSSKLTAKHGGAMRLGNVEGLSVEFSPGTLPAGSSEAELLIKDLLDDLVSESMDLHTTNFWSDP-AMLGEHLVTPGGAIRRRDWSKAATMVHELSKSEHTSPANICSWHHVHAIHVASDFVLISGLNHLLVFGGGPDGGKNQPTELDVACFMGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFIDGDGMEVQHGYYFDELGLLQGGSSTGSSLFGFPEFHGGDFFYDSDRRKIIQYIDMVKSDSIQGSFGSSFTVSGIAFPWIPADDFGEDDEAGHGTHTAGTAAGATLNNPAET-VTCEGGQTLGCAGACIDA--TGSDDDLVTSF--YQRYDVD--RQCPIFGCDDDTEA-CLGDAVGTTLAENGGMAQGAKLSIFDVFLGELGLTSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDGENPEN-SVCTIGSPAIAKNVLAVGATSSGETRFN-----------TDGVYDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGTDDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDD-GFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWS-SGEKDNVNVNERVVVAAEDVESGTWT 4756
            R   ++F +LAA    WG +A++ PYPSL  VE +LLTTA+D F G  K FE A +W GE +R+       ++ +  APH  C  Y  G +   RLQ+LLSP AVRP +HSS HGACFF TASH +  A+ S+   +GL S++PFPSALK+APG++EH  +   +      +LT  HG  MR GNVEGL++E SPGTLPA S EA + I +LLDDL+S+S+DLH  N WSDP A  GEHL TP GA+R+R+WS AAT+VH+L+ +  TSP +ICSW  +   +  +D +L+SGL+HLL    G  G   + TEL VACFMG++SFL++R EVLRVSP H+ R L+A AR IIQ+AT TE PLT AGLDGTG++IQV+D+GLDETSCFFID DG EV HG+Y++ LG +                                                           IP+     DD  GHGTH AG+A GAT+  PAET  TC G +  GC G CIDA  T   DDL T    Y  YD+D  R CP+  CDD T+  CL D VG TLA++GGMAQGAKL+ FDVF      T F  NGLWEPCLEAGCKIHS S G    C++   D+ YD+FMY NPENL++ AAGN G+  +  +VCT+ SPAI KN LAVGATSSGETR             T+G  D+D VA FSS+G T DGRIKPEVVAPGD IYSA SDG+D+HSCRL A  GTSM+ P+VAGASAM+RQYF+D  FY AD  +RG+ DD G  CE FSPSSATVKALLINSANLMGG+SEPDGYRGFGRIHLE GMPLDGEG LALFVADS+ TS+ E    +++FDVDA+A++DFRATLSWID PA++ SGVQL+HDLDL V+SPSG ++ MW+ SGE D+ NVNERV++AAEDVESGT+T
Sbjct:    6 RTATALFFSLAAASTPWGGSANTLPYPSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRS------KEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAM-SDSPYYGLTSISPFPSALKVAPGVLEHGVSL-DQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALGEI-----------------------------------------------------------IPSSSSARDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 823 bits (2125), Expect = 2.980e-268
Identity = 472/975 (48.41%), Postives = 614/975 (62.97%), Query Frame = 2

Query: 2009 MRATFSVFVTLAALWGVAADSFPYPSLRAVENTLLTTAKDDFAGQRKGFENAVRWDGERIRAGIRFNDSDQGDVLAPHFACAGYGHGREAFLRLQQLLSPEAVRPASHSSEHGACFFATASHTQVAAIISEHDEFGLESLAPFPSALKLAPGLIEHEANDGSEPIN---------LSSKLTAKHGGAMRLGNVEGLSVEFSPGTLPA---GSSEAELLIKDLLDDLVSESMDLHTTNFWSDPAMLG-EHLVTPGGAIRRRDWSKAATMVHELSKSEHTSPANICSWHHVHAIHVASDFVLISGLNHLLVFGGGPDGG-KNQPTELDVACFMGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFIDGDGMEVQHGYYFDELGL---LQGGSSTGSSLFGFPEFHGGDFFYDSDRRKIIQYIDMVKSDSIQGSFGSSFTVSGIAFPWIPADDFGEDDEAGHGTHTAGTAAGATLNNPAETVTC-EGGQTLGCAGACI--DATGSDDDLVTSFYQRYDVDRQCPIFGCDDDTEACLGDAVGTTLAENGGMAQGAKLSIFDVFLGELGLTSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDGENPENSVCTIGSPAIAKNVLAVGATSSGETRFNT-----------DGVYDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGTDDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDDGFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWSSGEKDNVNVNERVVVAAEDVE----SGTWTVSVSANSLEITDRQSYSLVVNGAL 4828
            MR TF    T   L    + +   PSL   E TLLTTA  DF  +   F  A  W     +AG R   S      +P+ +C  +G GR A+ +L+  L   AV+P S ++ HGACF  TAS  + A + S  D     +   FP+ALK+APGL++H + DG               + +L+  HG +M++ +  GL VE SPGTLP+   G ++A   I++LL  L S S+DLH    WSD  M G +HL +  GA+R R+WS+AAT+V+E S+SE  +P+ +C W  V   H A+D +L++GL+HLL  G    G  + +  EL +AC+MG++SFLASR EVL+V+  + K  L+A ARA IQ+A+   TPLT AGLDG+GE+IQ++D+G++ET+CF   GDG EV+HGYYFD +     L   SS   S+F   EF GGDF YDSDRRK+IQYID++  D+  GS       SG+  P++ AD F  DD+ GHGTH A T AG+T+++PAE V+C +G + +GCAG CI   +TGS +    + Y   D++R CP FGC  D   CL D    TL+E+GGMAQGAK++  DVF G+      A NGLWE C +AGCKIHSNS+G    C L A D+ YD +MYEN E+LL+F+AGN G   +   CTI SP IAKNVL VG+TSSG TR +T           D +  ID V+ FS +GFT DGRIKPEVVAPGDMIYSA+ +G D  SC L AY GTS ST V +GA+AM+RQYF D+ FY+ADV SRGFCD GF C+ FSP + TVKA+LINSAN+MGG +EPD  RGFGRIHLE GMPL G GD+ALFVADS+   +       + F+VD DA ++ RATLSW D  A++ SG QL++DLDL V +P GT Y MW     D  NVNERV+V AE V     +  W V V      + D  S+SLVV GA+
Sbjct:    1 MRVTFRGAATSVLLISTVSGASQVPSLGFFEQTLLTTAGADFDSEA-AFAFASAW-----QAGDRQGRS------SPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWED---FTTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFK-AEFTGGDFSYDSDRRKVIQYIDLINPDTGDGSS------SGM--PFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSE----ADYGE-DINRLCPAFGCSGDQ--CLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAI 943          

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 784 bits (2024), Expect = 1.680e-257
Identity = 422/665 (63.46%), Postives = 495/665 (74.44%), Query Frame = 2

Query: 2873 SGLNHLLVFGGGPDGG-KNQPTELDVACFMGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFIDGDGMEVQHGYYFDELGLLQGGSSTGSSLFGFPEFHGGDFFYDSDRRKIIQYIDMVKSDSIQGSFGSSFTVSGIAFPWIPADDFGEDDEAGHG--------THTAGTAAGATLNNPAETVTCEGGQTLGCAGACIDATGSDDDLVTSFYQR--YDVDRQCPIFGCDDDTEA-CLGDAVGTTLAENGGMAQGAKLSIFDVFLGELGLTSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDGENPENSVCTIGSPAIAKNVLAVGATSSGETR-FNTDGVYDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGTDDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDDGFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWSSGEKDNVNVNERVVVAAEDVESGTWTVSVSANSLEITDRQSYSLVVNGAL 4828
            +GL+HLL  G G  G  + + TEL VACFMG++S LA RPEVLRVSP  +KR L+A AR I+Q+A++T+TPLT AGLDGTGEIIQV+DSGLDETSC+FID  G EV HGYYFDEL      SS  SS      F+GGDF Y  +RRKIIQYID+V  DS   SF +S   S I + ++P D FGEDDEA                +AAGATLNNPAET+TC    TL C GACID     DDLV+  +Q    D+DR CP++GCD+ T+  CL D V  TL+ NGGMAQGAKL+IFDVF G+   +S   N LWE C EAGCK+HSNS G    C++ + D+  D FMYEN E+LLIFAAGN+G   E S CTI +P I KN LA+GA++SG+TR  +T G   I+ VA FSSWGFTTD RIKPEVVAPGD +YSAASDGTD HSCRLWAYEGTSMS P+VAGA+AMIRQYF+D  FY ADVT+RGFC D F+CEGFSPS+ATVKALLINSANLMGG+SEPDG+RGFGR+HLE GMPL+GEG+L LFVAD+  TSI+E +L  YLFDVDADA +D RATLSWID P+T  S VQL+H+LDL V+SP GT+Y MW S   DN NVNERV+V A DV +GTWTV V ANSL ITD QSYSLVVNGA+
Sbjct:    4 TGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELAQAAVYSSLYSSDETATIFNGGDFSYYPERRKIIQYIDLVNPDSPYSSFYTS-QGSYIGY-FLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACIDLLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEG-GEERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSL-ITDSQSYSLVVNGAI 664          

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 748 bits (1930), Expect = 2.440e-243
Identity = 407/685 (59.42%), Postives = 493/685 (71.97%), Query Frame = 2

Query: 2957 MGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFI-DGDGMEVQHGYYFDELGLLQGGSSTGSSLFGFP----------------------------EFHGGDFFYDSDRRKIIQYIDMVKSDSIQGSFGSSFTVS-GIAFPWIPADDFGEDDEAGHGTHTAGTAAGATLNNPAETVTCEGGQTLGCAGACIDATGSDD--DLVTSFYQRYDVDRQCPIFG---CDD-DTEACLGDAVGTTLAENGGMAQGAKLSIFDVFLGELGLTSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDGENPENSVCTIGSPAIAKNVLAVGATSSGETRFNTDGVYDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGT-DDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDDGFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWSSGEKDNVNVNERVVVAAEDVESGTWTVSVSANSLEITDRQSYSLVVNGAL-----IGANVMVDNSSPASSVVSN 4885
            MG++SFLAS+PEVLRV+P  S+  L+A ARA IQ+AT T+TPLT AGLDGTG+++QV+DSG+DE+SCFF  D  G  V HG+YF+E G+     S  SS F  P                            EF GGDF    DRRKIIQYI+++KSD  +GS G SFT S G  +PW+ AD F  DDEAGHGTH AG+ AGATLN PAE ++C   +T+ C GAC+D   ++D  DL T + +  D+DR CP+F    CD  +T+ CL D V  TL  NGGMAQGAKL+IFD F    GL+ F   GLWEPC +A CKIHSNS+GG   C+LG+ND+ YD +M  NPENLLIFAAGNDGE    S CTIGSPAIAKNVLAVGA++SG+TR +      ID VA FSS+G T+D RIKPEV+APGD IYSAASDG+ D HSCRLWAY GTSMSTP+VAGASAMIRQYF++  F+A D+T+RGFCD+   CE FSPSSAT+KAL+INSANLMG +SEP+G RGFGRIHLE GMPL GEG LALFV+DS  TSI E +   Y FDV+A A +D R TLSW+D PATTFS VQL+HDLDL V+SP+GT YTMW+SG  D+ NVNERV+V A+DVE+GTW+V + A++L  TD QSYSLVVNGA+     +GA   ++ SS  SS  S+
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGI---NPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGEL-RRSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGSGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLT-TDVQSYSLVVNGAISPGTGVGA---IERSSSTSSTSSD 677          

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 728 bits (1878), Expect = 1.810e-230
Identity = 472/1073 (43.99%), Postives = 615/1073 (57.32%), Query Frame = 2

Query: 2060 AADSFPYPSLRAVENTLLTTAKDDFAGQRKGFENAVRWDGERIRAGIRFNDSDQGDVLAPHFACAGYGHGREAF-LRLQQLLSPEAV--RPASHSSEHGACFFATASHTQVAAIISEHDEFGLESLAPFPSALKLAPGLIEHEANDGSEPINLSSKLTAKHGGAMRL----------------------------------------------GNVEGLSVEFSPGTLPA----GSS------EAELLIKDLLDDLVSESMDLHTTNFWSD--PAMLGEHLVTPG---------GAIRRRDWSKAATMVHELSKSEH-TSPANICSWHHVHAIHVASDFVLISGLNHLLVFGGGPDGGKN-QPTELDVACFMGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFIDGDGME-VQHGYYFDELGLLQGGSSTGSSLFGFPE-----FHGGDFFYDSDRRKIIQYIDMVK----SDSIQGSF-----------------------GSSFTVS-GIAFPWIPADDFGEDDEAGHGTHTAGTAAGATLNNPAETVT------CEGGQTLGCAGACIDATG--SDDDLVTSF--------YQRYDVDRQCPIFGCDD-DTEACLGDAVGTTLAENGGMAQGAKLSIFDVFLGELGL-TSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDGENPENSVCTIGSPAIAKNVLAVGATSSGETRFN--------TDG---VYDIDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGTDDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDDGFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWSSGEKDNVNVNERVVV--------------AAEDVES-GTWTVSVSANSLEITDRQSYSLVVNGAL 4828
            AA + P+P L  +   LLTTA   F  Q + F+ AV W   R+      +D+      AP+ ACA     R      L  +L   A+  R    S+EHG C  ATA+  Q  +I      F   S A FPSALKLAPGL++H  +D  +      +L   HG  +R                                                 V GL+VE +PG L A    GSS       +  L  +  ++L+S+S+D+H  +FWS    A L +     G         G +  R+W++AA ++  L  +E   +  ++CSW  +  +H   D + + G++HLL  G G  G +  +  EL VACFM ++SFLASRPEVLRVSP  + + L+A A   +Q+A+ TE+P+T AGLDGTGE+IQ+VD+GLD  SCFF+D DG E V HGYYF+E+G+      +  + + F       F GGDF +D  RRK +  +D       S +I   F                       G S+  S G  +PWI A +F EDD  GHGTHTAG+AAGA +++P+ + T      CE G+ L C G CID      +DDL+T++        Y+  D+DR CP   CDD   E CLGD    TL ++GG+A+GA+L+IFD+     G+    A NG+WEP +EAG K+ SNSWG    C   + D+ +D+FMY N + LL+FAAGNDG+  + S CT+G+PA+AKNVLAVGA++SG  R +        TDG   V DID +A FSS+G T D RIKPEVVAPGDMIYSAA  G  +++CRLW Y GTSMSTP+VAGA+A++RQYF +  FYA DV  RGFCD  F CE F+PS+ATVKA+LINSANLMGG++EPDG RGFGR+HLE GMPL GEGD ALFVADSA TS +E +L  Y F VDA A ID RATLSWID PATTFS VQL+HDLDL+V SP+G  Y+MW  G  D++N NERV+V               AED ES GTWTV V +  L   + QSYSLVV GA+
Sbjct:   23 AAPTLPHPPLSVIRGALLTTAGVTFRHQGE-FKAAVEWHEARL------DDARP----APYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFS--SWAAFPSALKLAPGLLDHP-HDDRDGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGL-VVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGD--DWSGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCDS-FGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAI 1077          

BLAST of mRNA_H-canaliculatus_F_contig117.1509.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 698 bits (1801), Expect = 5.400e-221
Identity = 449/1035 (43.38%), Postives = 585/1035 (56.52%), Query Frame = 2

Query: 2087 LRAVENTLLTTAKDDFAGQRKGFENAVRWDGERIRAGIRFNDSDQGDVLAPHFACAGYGHGREAFLRLQQLLSPEAVRPASHSSEHGACFFATASHTQVAAIISEHDEFGLESLAPFPSALKLAPGLIEHE--------ANDGSEPINLSSKLTAKHGGAMRLGNVEGLSVEFSPGTLPAGSSE--AELLIKDLLDDLVSESMDLHTTNFWSDP-AMLGEHLVTPGGAIRRRDWSKAATMVHELSKSEHTSPANICSWHHVHAIHVASDFVLISGLNHLLVFGGGPDGGKNQPTELDVACFMGVISFLASRPEVLRVSPTHSKRPLDATARAIIQTATSTETPLTAAGLDGTGEIIQVVDSGLDETSCFFIDGDGMEVQHGYYFDELGLLQGGS-STGSSLFGFPEFHGGDFFYDSDRRKIIQYIDMVKSDSIQGSFGS---SFTVSG--IAFPW-------IPADD------------------------------------------------FGEDDEAGHGTHTAGTAAGA-TLNNPAETVTCEGGQTLGCAGACIDATGSDDDLVTSFYQRYDVDRQCPIFGCDDDTEA----CLGDAVGTTLAENGGMAQGAKLSIFDV-FLGELGLTSFASNGLWEPCLEAGCKIHSNSWGGAGACALGANDLQYDNFMYENPENLLIFAAGNDG---ENPENSVCTIGSPAIAKNVLAVGATSSGE---TRFNTDG--VYD--------------------------------IDHVASFSSWGFTTDGRIKPEVVAPGDMIYSAASDGTDDHSCRLWAYEGTSMSTPVVAGASAMIRQYFVDAKFYAADVTSRGFCDD---GFLCEGFSPSSATVKALLINSANLMGGASEPDGYRGFGRIHLEQGMPLDGEGDLALFVADSATTSISESSLHNYLFDVDADADIDFRATLSWIDYPATTFSGVQLIHDLDLTVLSPSGTSYTMWSSGEKDNVNVNERVVVAAEDVESGTWTVSVSANSLEITDRQSYSLVVNGAL 4828
            L  ++    ++A DDFA  +  +  A  W  +R+       D+ +G   AP+ ACA Y  GR A   L+   +  AV   S++   G CF  TA  +   A+I   ++F L S AP   +LKLA  L++H         A D  +  + S+ L + +G  M L NV GLSV  SPG L AG  E  AE  ++D   DL SES+++   +FWSD  A   E+ VT     R R+WS+AA +V  L+     +   IC +  +   HVA D VL+ G+NHLL        GK  P+E   AC+MG++S LA++PEVLRVSP  S R  +A A A++Q+AT+TETPL  AGLDGTGE+IQVVDSGLDETSCFF D DG++V+HG+ FD + +   G+ ST    + FP        +D  RRKI+QYI + K+   QGS  S   SFT     +  P+       I  DD                                                F +D   GHGT TAG+AAGA +  +  E   C G +  GCAG CI A+  D  L  S    +D+D  CP++ CD +T+     CL D     L ++GG+A GA++S+FDV ++G     SFA N LW   +E G KIH+NSWGGA  C L  ++  YD FMYENPE+LLIFAAGNDG   + P    CT+GSPA+ KN LAVGATSSG    TR   DG  VY+                                +D VA FSS G T DGRIKP+VVAPGD + SA+SDGTD HSC+L A  GTS S P+VAGA+A++RQYF D  F+A DV SRG C +      C  F+PS+ATVKA++INSA+LMGG+SEP+G RGFGR+HLE GMP+DG G+  L V ++A   I++  +   L  VD DA ++ RATL+W+D P T  S  QL HDLDL V++PSG  YTMW SG  D VNV ERV+V  E +ESG W+V VSA +L +TD Q YSLVV GA+
Sbjct:   14 LGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSY-----DAAEGST-APYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVT-----RVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLP-------GKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFP--------FDMSRRKIVQYIQLFKTPD-QGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDS---TFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAA---IADGEITE-LVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKAL-LTDEQLYSLVVTGAI 1013