mRNA_H-canaliculatus_F_contig10293.308.1 (mRNA) Hapterophycus canaliculatus Oshoro5f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-canaliculatus_F_contig10293.308.1
Unique NamemRNA_H-canaliculatus_F_contig10293.308.1
TypemRNA
OrganismHapterophycus canaliculatus Oshoro5f female (Hapterophycus canaliculatus Oshoro5f female)
Homology
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: A0A6H5KEW6_9PHAE (Isochorismatase domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KEW6_9PHAE)

HSP 1 Score: 239 bits (611), Expect = 5.300e-78
Identity = 113/139 (81.29%), Postives = 126/139 (90.65%), Query Frame = 1
Query:    1 HKFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAVEQL 417
            HK +  PEGKRIGD GPMGRILVRGERGHDI+PELYPLPMEP VDKPGKGAFF+TDL TIL+SRGI+TL+VCGVTTEVCV +TVREANDRGF+CVV DEACSSFFPEFH  GL+M+ AQGGI GRVASVA+VV+AV +L
Sbjct:   90 HKLMQSPEGKRIGDLGPMGRILVRGERGHDIIPELYPLPMEPCVDKPGKGAFFSTDLETILRSRGIETLIVCGVTTEVCVHTTVREANDRGFACVVVDEACSSFFPEFHRVGLSMVMAQGGIFGRVASVASVVRAVREL 228          
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: A0A127ENE2_9HYPH (Isochorismatase n=2 Tax=Bacteria TaxID=2 RepID=A0A127ENE2_9HYPH)

HSP 1 Score: 196 bits (498), Expect = 4.660e-61
Identity = 94/137 (68.61%), Postives = 107/137 (78.10%), Query Frame = 1
Query:    1 HKFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAVE 411
            HK   G    RIG PGPMGRILVRGE GHDI+PELYP P EPV+DKPGKGAF+ TDL  +L++R I TLLVCGVTTEVCV +TVREANDRGF CVV  + C+S+FPEFH AGLAMIKAQGGI G V     V+ A++
Sbjct:   86 HKVERGEPSLRIGQPGPMGRILVRGEPGHDIIPELYPAPGEPVIDKPGKGAFYQTDLELMLKNREIDTLLVCGVTTEVCVNTTVREANDRGFRCVVLSDCCASYFPEFHEAGLAMIKAQGGIFGWVTPSTKVLAALD 222          
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: A0A4Q5XBK4_9DELT (Cysteine hydrolase n=1 Tax=Myxococcales bacterium TaxID=2026763 RepID=A0A4Q5XBK4_9DELT)

HSP 1 Score: 194 bits (494), Expect = 1.830e-60
Identity = 91/135 (67.41%), Postives = 108/135 (80.00%), Query Frame = 1
Query:    4 KFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAV 408
            K   G    RIG PGPMGRILVRGE GHD++PELYPLP EPV+DKPGKGAF+ATDL +ILQ RGI+TL+VCGVTTEVCV +TVREANDRG+ C+V  + C+S+FPEFH  GL MIKAQGGI G V+    + +A+
Sbjct:   88 KVERGAPSMRIGSPGPMGRILVRGEPGHDLIPELYPLPGEPVIDKPGKGAFYATDLGSILQHRGIETLIVCGVTTEVCVHTTVREANDRGYKCIVPGDCCASYFPEFHEMGLRMIKAQGGIFGWVSDSQKLQRAL 222          
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: A0A3S2VTA8_9HYPH (Cysteine hydrolase n=1 Tax=Methylobacterium oryzihabitans TaxID=2499852 RepID=A0A3S2VTA8_9HYPH)

HSP 1 Score: 194 bits (494), Expect = 1.880e-60
Identity = 94/137 (68.61%), Postives = 109/137 (79.56%), Query Frame = 1
Query:    4 KFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAVEQ 414
            K   G    RIG PGPMGRIL+RGE GHDIVP L P P EPV+DKPGKGAF+ATDL  IL++RG+Q LLVCGVTTEVCV +TVREANDRG+ CVV  +AC+S+FPEFH AGLAM+KAQGGI G V++ A  V A+ Q
Sbjct:   86 KLERGEPSARIGAPGPMGRILIRGEPGHDIVPALAPRPGEPVIDKPGKGAFYATDLGAILEARGVQALLVCGVTTEVCVHTTVREANDRGYRCVVIADACASYFPEFHEAGLAMVKAQGGIFGWVSTSAAAVAALRQ 222          
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: UPI00131E81D1 (cysteine hydrolase n=1 Tax=Acidisphaera sp. S103 TaxID=1747223 RepID=UPI00131E81D1)

HSP 1 Score: 194 bits (494), Expect = 2.200e-60
Identity = 92/136 (67.65%), Postives = 108/136 (79.41%), Query Frame = 1
Query:    4 KFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAVE 411
            K + G    RIGDPGPMGRILVRGE GHDI+PELYP   EPV+DKPGKGAFFATDLH ILQ++GIQ L+V GVTTEVCV +TVREANDRG+ C+V  + C S+FPEFH  GL MI AQGGI G VA  A +++A++
Sbjct:   84 KKIRGRGATRIGDPGPMGRILVRGEAGHDIIPELYPAAGEPVIDKPGKGAFFATDLHAILQNQGIQQLVVTGVTTEVCVNTTVREANDRGYDCLVVSDCCGSYFPEFHEMGLRMIAAQGGIFGWVAPSATIIQALK 219          
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: A0A1I4Q0T1_9HYPH (Nicotinamidase-related amidase n=5 Tax=Methylobacterium TaxID=407 RepID=A0A1I4Q0T1_9HYPH)

HSP 1 Score: 194 bits (493), Expect = 3.530e-60
Identity = 95/136 (69.85%), Postives = 106/136 (77.94%), Query Frame = 1
Query:    4 KFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAVE 411
            K   G    RIGDPGPMGRIL+RGE GHDIVPEL P P EPV+DKPGKGAF+ATDL +IL +R I TLLVCGVTTEVCV +TVREANDRG+ C V  +AC S+ PEFH AGLAMIKAQGGI G V   A VV A++
Sbjct:   93 KLARGEPSARIGDPGPMGRILIRGEAGHDIVPELRPEPGEPVIDKPGKGAFYATDLASILSARRIDTLLVCGVTTEVCVHTTVREANDRGYRCAVVADACGSYIPEFHAAGLAMIKAQGGIFGWVTDSAAVVDALD 228          
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: A0A2S6NMF0_RHOGL (Isochorismatase n=1 Tax=Rhodopila globiformis TaxID=1071 RepID=A0A2S6NMF0_RHOGL)

HSP 1 Score: 193 bits (491), Expect = 5.210e-60
Identity = 94/136 (69.12%), Postives = 108/136 (79.41%), Query Frame = 1
Query:    4 KFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAVE 411
            K+  G    RIGDPGPMGRILVRGE GHDI+PELYP   EPVVDKPGKGAF ATDLH ILQ+RGI  L+V GVTTEVCV +TVREANDRG+ C+V  + C+S+FPEFH AGL MIKAQGGI G VAS   V++ ++
Sbjct:   84 KYRRGRGETRIGDPGPMGRILVRGEPGHDIIPELYPAAGEPVVDKPGKGAFHATDLHAILQNRGIAQLVVTGVTTEVCVNTTVREANDRGYDCLVVADCCASYFPEFHDAGLRMIKAQGGIFGWVASSGAVLQVIK 219          
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: UPI001BCC76C6 (cysteine hydrolase n=1 Tax=Roseococcus sp. SDR TaxID=2835532 RepID=UPI001BCC76C6)

HSP 1 Score: 193 bits (491), Expect = 5.550e-60
Identity = 95/137 (69.34%), Postives = 107/137 (78.10%), Query Frame = 1
Query:    4 KFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAVEQ 414
            K    P G RIGDPGPMGRILVRGE GHDI+PEL PLP E V+DKPGKGAF+ATDL  IL++RGI+ LLV GVTTEVCV +TVREANDRGF C V  +AC+S+FP FHTA LAMI AQGGI G VAS A    A+ +
Sbjct:   86 KLTRSPPGMRIGDPGPMGRILVRGEAGHDIIPELAPLPGEVVLDKPGKGAFWATDLENILRNRGIEALLVAGVTTEVCVSTTVREANDRGFRCAVISDACASYFPAFHTAALAMIHAQGGIFGWVASSAAATTALRE 222          
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: A0A4R5QD80_9PROT (Cysteine hydrolase n=1 Tax=Dankookia rubra TaxID=1442381 RepID=A0A4R5QD80_9PROT)

HSP 1 Score: 192 bits (487), Expect = 2.240e-59
Identity = 97/139 (69.78%), Postives = 109/139 (78.42%), Query Frame = 1
Query:    4 KFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGG----ILGRVASVANVVKAV 408
            K+  GP G+RIGDPGPMGRILVRGE GHDIVPEL P P E V+DKPGKGAF  TDL  IL++RGI+TLLVCGVTTEVCV +TVREANDRG+ CVV  + C S+FPEFH  GLAMIKAQGG    I G VA  A  V+A+
Sbjct:   86 KWERGPPGRRIGDPGPMGRILVRGEPGHDIVPELVPAPGEVVLDKPGKGAFCETDLDLILRNRGIRTLLVCGVTTEVCVNTTVREANDRGYRCVVLADCCGSYFPEFHATGLAMIKAQGGAGGGIFGWVADSAAAVRAL 224          
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Match: A0A354V7H4_9PROT (Isochorismatase n=1 Tax=Acetobacteraceae bacterium TaxID=1909293 RepID=A0A354V7H4_9PROT)

HSP 1 Score: 191 bits (484), Expect = 7.240e-59
Identity = 92/135 (68.15%), Postives = 105/135 (77.78%), Query Frame = 1
Query:    4 KFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKGAFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEACSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAV 408
            K + G     IGDPGPMGRILVRGE GHDI+PELYP   EPV+DKPGKGAFFATDLH ILQ+RGI  L+V GVTTEVCV +TVREANDRG+ C+V  + C S+FPEFH  GL MI AQGGI G VA  A V++A+
Sbjct:   84 KKVRGRSATCIGDPGPMGRILVRGEPGHDIIPELYPAAGEPVIDKPGKGAFFATDLHAILQNRGITQLVVTGVTTEVCVNTTVREANDRGYDCLVVSDCCGSYFPEFHEMGLRMIAAQGGIFGWVAPSATVIEAL 218          
The following BLAST results are available for this feature:
BLAST of mRNA_H-canaliculatus_F_contig10293.308.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KEW6_9PHAE5.300e-7881.29Isochorismatase domain-containing protein n=2 Tax=... [more]
A0A127ENE2_9HYPH4.660e-6168.61Isochorismatase n=2 Tax=Bacteria TaxID=2 RepID=A0A... [more]
A0A4Q5XBK4_9DELT1.830e-6067.41Cysteine hydrolase n=1 Tax=Myxococcales bacterium ... [more]
A0A3S2VTA8_9HYPH1.880e-6068.61Cysteine hydrolase n=1 Tax=Methylobacterium oryzih... [more]
UPI00131E81D12.200e-6067.65cysteine hydrolase n=1 Tax=Acidisphaera sp. S103 T... [more]
A0A1I4Q0T1_9HYPH3.530e-6069.85Nicotinamidase-related amidase n=5 Tax=Methylobact... [more]
A0A2S6NMF0_RHOGL5.210e-6069.12Isochorismatase n=1 Tax=Rhodopila globiformis TaxI... [more]
UPI001BCC76C65.550e-6069.34cysteine hydrolase n=1 Tax=Roseococcus sp. SDR Tax... [more]
A0A4R5QD80_9PROT2.240e-5969.78Cysteine hydrolase n=1 Tax=Dankookia rubra TaxID=1... [more]
A0A354V7H4_9PROT7.240e-5968.15Isochorismatase n=1 Tax=Acetobacteraceae bacterium... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-canaliculatus_F_contig10293contigH-canaliculatus_F_contig10293:3612..4791 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Hapterophycus canaliculatus Oshoro5f female vs UniRef902022-09-19
OGS1.0 of Hapterophycus canaliculatus Oshoro5f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score244.2
Seed ortholog evalue3.7e-62
Seed eggNOG ortholog2880.D8LSP5
EggNOG free text desc.Isochorismatase family
EggNOG OGs2A8EQ@1,2RYGB@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons2
Model size426
Cds size426
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928663.2899418-CDS-H-canaliculatus_F_contig10293:3611..38061622928663.2899418-CDS-H-canaliculatus_F_contig10293:3611..3806Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig10293 3612..3806 -
1696845517.6902957-CDS-H-canaliculatus_F_contig10293:3611..38061696845517.6902957-CDS-H-canaliculatus_F_contig10293:3611..3806Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig10293 3612..3806 -
1622928663.3043516-CDS-H-canaliculatus_F_contig10293:4560..47911622928663.3043516-CDS-H-canaliculatus_F_contig10293:4560..4791Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig10293 4561..4791 -
1696845517.704487-CDS-H-canaliculatus_F_contig10293:4560..47911696845517.704487-CDS-H-canaliculatus_F_contig10293:4560..4791Hapterophycus canaliculatus Oshoro5f femaleCDSH-canaliculatus_F_contig10293 4561..4791 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-canaliculatus_F_contig10293.308.1prot_H-canaliculatus_F_contig10293.308.1Hapterophycus canaliculatus Oshoro5f femalepolypeptideH-canaliculatus_F_contig10293 3612..4791 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-canaliculatus_F_contig10293.308.1

>prot_H-canaliculatus_F_contig10293.308.1 ID=prot_H-canaliculatus_F_contig10293.308.1|Name=mRNA_H-canaliculatus_F_contig10293.308.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=polypeptide|length=142bp
HKFLHGPEGKRIGDPGPMGRILVRGERGHDIVPELYPLPMEPVVDKPGKG
AFFATDLHTILQSRGIQTLLVCGVTTEVCVQSTVREANDRGFSCVVADEA
CSSFFPEFHTAGLAMIKAQGGILGRVASVANVVKAVEQLRS*
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mRNA from alignment at H-canaliculatus_F_contig10293:3612..4791-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-canaliculatus_F_contig10293.308.1 ID=mRNA_H-canaliculatus_F_contig10293.308.1|Name=mRNA_H-canaliculatus_F_contig10293.308.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=mRNA|length=1180bp|location=Sequence derived from alignment at H-canaliculatus_F_contig10293:3612..4791- (Hapterophycus canaliculatus Oshoro5f female)
CACAAGTTTCTTCACGGCCCCGAGGGAAAGCGCATCGGCGACCCCGGTCC CATGGGCCGCATCCTCGTCCGCGGGGAGAGGGGGCACGACATCGTGCCGG AGCTGTACCCCCTCCCGATGGAACCAGTAGTTGACAAGCCCGGCAAGGGG GCTTTCTTCGCCACCGACCTTCACACCATCCTACAATCCCGCGGGATTCA GACGCTCCTCGTTTGCGGCGTTACCACAGAGGTGAGCAACACAGACAGAT AGGGGGGAGGGGGGGGGGTCGGCGAAGGGAGTTCGTACAGTGGGCGGTGT TGGTGTCGTTTGCTGGTCGCGCTGTGGGGTAGGGTAGGGTTCGGTGCAGT GCAGGTCGCTACATAAACCGTGCCTTTTTTGCGGCGTGGTTTAGGTCGGG ACGCCGCCGTGGGAGGTCCGATGATTGCCTTCCCAACTTACGGAGGGCCT ACGCGCTGCTCGTTCGACTGCGGTAATACTAAGACCGACCGGGTAGCCTC TGTCGTTGTGAAGACTATTGCCGAAGTTTCCACTACAAAGGGAAGGCAGA GGGGGAGGTGGGTGGTGGCCCCCAAAAATCGAGCACTTGTACATAACCTG TCTTGAAGTGTTCCTTCGTACGTTGACGTAGACATGATTGGTAGTTGCGC GAATTGACCGCGCTGCTAACGGACGAAGGAAAGTGTGGGACAATTCACAG GAAAGCCTCAAAGGGACAGCTTCAAAGCTGAAGCAAAAGCTGACGTACGG TTTGTAAAGCCTCGACAATGGTGGTTCCGGCAGATGGTGAACAATCTTGG AGGTCAACCCACTCGAGTAGTCCGCACTTGTTCGCGCGCAGCTAATGGCA GCAGCAGCTAATGACAGCAGCAACAACAACAACAACAACAACAACAACAC TGCACTAGTGATAAAACAAAAGTAACAACGGCGGCAACGGCGACGATAGC AACAACACTAACGACGACGTACTCCACCCCAACAGGTTTGCGTCCAAAGC ACCGTGCGGGAGGCCAACGACCGCGGGTTCTCCTGTGTCGTTGCGGACGA GGCGTGCTCCTCTTTCTTTCCGGAGTTCCACACCGCTGGGTTGGCGATGA TCAAGGCCCAGGGGGGGATTTTGGGCCGGGTTGCCTCCGTTGCGAACGTC GTAAAGGCGGTCGAGCAACTCCGTTCGTAA
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Coding sequence (CDS) from alignment at H-canaliculatus_F_contig10293:3612..4791-

>mRNA_H-canaliculatus_F_contig10293.308.1 ID=mRNA_H-canaliculatus_F_contig10293.308.1|Name=mRNA_H-canaliculatus_F_contig10293.308.1|organism=Hapterophycus canaliculatus Oshoro5f female|type=CDS|length=852bp|location=Sequence derived from alignment at H-canaliculatus_F_contig10293:3612..4791- (Hapterophycus canaliculatus Oshoro5f female)
CACAAGTTTCTTCACGGCCCCGAGGGAAAGCGCATCGGCGACCCCGGTCC
CATGGGCCGCATCCTCGTCCGCGGGGAGAGGGGGCACGACATCGTGCCGG
AGCTGTACCCCCTCCCGATGGAACCAGTAGTTGACAAGCCCGGCAAGGGG
GCTTTCTTCGCCACCGACCTTCACACCATCCTACAATCCCGCGGGATTCA
GACGCTCCTCGTTTGCGGCGTTACCACAGAGCACAAGTTTCTTCACGGCC
CCGAGGGAAAGCGCATCGGCGACCCCGGTCCCATGGGCCGCATCCTCGTC
CGCGGGGAGAGGGGGCACGACATCGTGCCGGAGCTGTACCCCCTCCCGAT
GGAACCAGTAGTTGACAAGCCCGGCAAGGGGGCTTTCTTCGCCACCGACC
TTCACACCATCCTACAATCCCGCGGGATTCAGACGCTCCTCGTTTGCGGC
GTTACCACAGAGGTTTGCGTCCAAAGCACCGTGCGGGAGGCCAACGACCG
CGGGTTCTCCTGTGTCGTTGCGGACGAGGCGTGCTCCTCTTTCTTTCCGG
AGTTCCACACCGCTGGGTTGGCGATGATCAAGGCCCAGGGGGGGATTTTG
GGCCGGGTTGCCTCCGTTGCGAACGTCGTAAAGGCGGTCGAGCAACTCCG
TTCGTAAGTTTGCGTCCAAAGCACCGTGCGGGAGGCCAACGACCGCGGGT
TCTCCTGTGTCGTTGCGGACGAGGCGTGCTCCTCTTTCTTTCCGGAGTTC
CACACCGCTGGGTTGGCGATGATCAAGGCCCAGGGGGGGATTTTGGGCCG
GGTTGCCTCCGTTGCGAACGTCGTAAAGGCGGTCGAGCAACTCCGTTCGT
AA
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