mRNA_H-paniculata_contig1053.348.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: D7FVJ5_ECTSI (tRNA:m(4)X modification enzyme TRM13 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVJ5_ECTSI)

HSP 1 Score: 428 bits (1100), Expect = 2.630e-139
Identity = 296/664 (44.58%), Postives = 366/664 (55.12%), Query Frame = 3

Query:   42 PDSGSVEERERGLSRKRRQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVSENAEFCVTHMPASSFADGNRRVPCPLDPNHNVAERLLESHVKICNKTRQSKELEQQPYFSKDINSGRPS-SGKDCDSDVTWDHAGEEAVGEPGNAIGNAAVFDEAFVLDLITRVSAAHREHVGDLSMEVLDPPQVQALHRTVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADKKLDGVSTRARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALRAMLTLPPGNIDPIVENTVGEGGNIEK-DPVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWISQEPESAWPSAPANLR----RNP--CSSLEMPQQQSGENGSPAPQQLRRPVNTDRMDSDHCEPMEHYDHGSGHVDRP--MPLFGV--GGPGQS----------GVEEDP-----------------------------------------------------------------GVLPAMSGAARAVAARECKRLLDRGRVWFVQEKLGLTCELVHFVEREVTPENALLLAFRA*E 1772
            P S S+EE+ R  SR+RRQE+K+AQ  ALKTG+W+RC +RVPGK R+CAQ R   +A FC THM     A+  +RVPCP+DPNH V E+ L SHVKICN   Q   +  QPY+S+ INSG P   G++                            D+AFV+DL++RV AAHR+ VG++  EVLDPP+VQALHR    G VG   G +        GH    G  GP    L+VEMGAG+G LGQ+IATAFPGS++TM+ERSS+R KA+ +L+GVSTRARIDIRDLNM GLPSLT  +G+ RPVVAVAKHLCGVATDLALRAMLTLPP            +GG++   DP + P                    A+A T   R GGVAIATCCHH C+WRDYVG++FL + GF+ARDF+AM+RISAWIS EP++    A  N R    R+P   SS    ++  GE  S A +  RRP    R      EP      G G   RP     +G   GGPG++          G  ED                                                                    L  ++ + +AVAAR CKRLLDRGR  F++E+LGL  E+VHFV  +VTPENALLL FRA E
Sbjct:    7 PRSKSIEEQTRADSRRRRQERKKAQLGALKTGVWDRCLYRVPGKGRYCAQHRSKHSASFCGTHM--GETAEKGKRVPCPVDPNHTVFEQQLSSHVKICNLALQQARMVAQPYYSEGINSGPPLLPGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXLDDAFVVDLLSRVEAAHRDLVGEIPTEVLDPPEVQALHR----GLVGPAGGDN--------GH----GENGPSV--LHVEMGAGKGTLGQSIATAFPGSDVTMVERSSVRRKAENRLEGVSTRARIDIRDLNMGGLPSLTA-EGDDRPVVAVAKHLCGVATDLALRAMLTLPPRP----ESRDADDGGHVGNGDPTAAP--------------------AAATT---RVGGVAIATCCHHVCNWRDYVGRDFLTQQGFSARDFEAMRRISAWISLEPDAG---ARKNNRYPDDRHPGVTSSPSFCRKTPGETASVAARS-RRPAAETRKLEPVLEP------GGGDCPRPGEREAYGWSGGGPGEAREGTWTEQLCGEREDSLERARETNRGHSSSPPWHTTPAPTFMTASTPSSLHSPTSDNANANTSIGDGSDVLXXXXXXXXXXXXLAPLTPSEKAVAARGCKRLLDRGRRAFIEERLGLRSEIVHFVGSDVTPENALLLGFRAEE 612          

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: A0A6H5KUX2_9PHAE (tRNA:m(4)X modification enzyme TRM13 n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KUX2_9PHAE)

HSP 1 Score: 393 bits (1009), Expect = 6.500e-125
Identity = 287/723 (39.70%), Postives = 361/723 (49.93%), Query Frame = 3

Query:   42 PDSGSVEERERGLSRKRRQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVSENAEFCVTHMPASSFADGNRRVPCPLDPNHNVAERLLESHVK--------------------------ICNKTRQSKELEQQPYFSKDINSGRP---SSGKDCDSDVTWDHAGEEAVGEPGNAIGNAAVFDEAFVLDLITRVSAAHREHVGDLSMEVLDPPQVQALHR-TVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADKKLDGVSTRARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALR---------------------------------------------------AMLTLPPGNIDPIVENTVGEGGNI-EKDPVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWISQEPESAWPSAPANLR----RNP--CSSLEMPQQQSGENGSPAPQQLRRPVNTDRM-----------------DSDHCE----------------------PMEHYDHGSGHVDRPMPLFGVGGPG---------------------QSGVEEDPGVLPAMSGAARAVAARECKRLLDRGRVWFVQEKLGLTCELVHFVEREVTPENALLLAFRA 1766
            P S S++E+ R  SR+RRQE+K AQ  AL+TG+WERC +RVPGK R+CAQ     +A FC THM     A+  +RVPCP+DPNH V E+ L SHVK                          +CN   Q   +  QPY+S+ INSG P                  G+ AV     A+G AA  D+AFV+DL++RV AAHR  VG++  EVLDPP+VQALHR  V   G G   G                G  GP    L+VEMGAG+G LGQ+IATAFPGS++TM+ERSS+R KA+ +L+GVSTRARIDIRDL+M GLPSLT   G+ RPVVAVAKHLCGVATDLALR                                                   AMLTLPP            +GG++   DP   P                    A+A T   R GGVAIATCCHH C+WRDYVG++FL++ GF+ARDF+AM+RISAWIS EP++    A  N R    R+P    SL  P++  GE  S A   LR    T ++                 ++D C                        +E     +G      P      P                       + + +   VL  ++ + +A AAR CKRLLDRGR  F++E+LGL  E+VHFV  +VTPENALLL FRA
Sbjct:    7 PRSKSIDEQTRADSRRRRQERKNAQLEALQTGVWERCLYRVPGKARYCAQH----SASFCGTHM--GETAEKGKRVPCPVDPNHTVFEQQLSSHVKRSRRVSPQAPPAAPYRTMWHVGSTTKVCNLALQQARMVTQPYYSEGINSGPPLLPGEXXXXXXXXXXXXRGDVAVVA---AVGAAADLDDAFVVDLLSRVEAAHRHLVGEIPTEVLDPPEVQALHRGLVGPTGAGGESGH---------------GEDGPSV--LHVEMGAGKGTLGQSIATAFPGSDVTMVERSSVRRKAENRLEGVSTRARIDIRDLDMGGLPSLTAA-GDDRPVVAVAKHLCGVATDLALRRDGGATDGETNREKHQAGHLPFHLLVPRHFPPWLAVLALSLRRSLPNLYRRAMLTLPPRR----ESRDADDGGHVCNGDPTDAP--------------------AAATT---RVGGVAIATCCHHVCNWRDYVGRDFLMQQGFSARDFEAMRRISAWISLEPDAG---ARKNNRYPDDRHPGVTFSLSFPRKIPGETASVAAGSLRPAAETRKLQPVLEPGGGDGPWPGEREADSCSGGGPGEAREGIWTEQFSGEGENSLERAGETNGGHSSSSPWHATAAPSLNMSASTPSSLQLPTSDNANASTSIGDGSEVLVPLTPSEKAAAARGCKRLLDRGRRAFIEERLGLCSEIVHFVGSDVTPENALLLGFRA 672          

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: A0A835ZI09_9STRA (tRNA:m(4)X modification enzyme TRM13 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZI09_9STRA)

HSP 1 Score: 244 bits (623), Expect = 3.700e-69
Identity = 197/599 (32.89%), Postives = 281/599 (46.91%), Query Frame = 3

Query:   42 PDSGSVEERERGLSRKRRQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVSENAEFCVTHMPASSFADGNRRVPCPLDPNHNVAERLLESHVKICNKTRQSKELEQQPYFSKDINSGRPSSGKD--CDSDVTW-------DHAGEEAVGEPGNAIGNAAVFDEA----------FVLDLITRVSAAHREHVGDLSMEVLDPPQVQALHRTVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADKKLDGVST---RARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALRAMLTLPPGNI--DPIVENTVGEGGNIEKD--PVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWISQEPESAWPSAPANLRRNPCSSLEMPQQQSGENGSPAPQQLRRPVNTDRMDSDHCEPMEHYDHGSGHVDRPMPLFGVGGPGQSGVEEDPGVLPAMSGAARAVAARECKRLLDRGRVWFVQEKLGLTCELVHFVEREVTPENALLLAF 1760
            P  G+V+        +R+  KK A Q +  TG W+ CR  V  K R+C+Q R   +A++C  H       D   R+PCP+D  H++ +  L+ H+KIC  T+ + ++  Q ++SK INSG P +G      +DV         D A +    +    I  +AV+  A          ++ DLI R+  A+ EHVG +S   L PP+ + L        +  ++ RH++QQ+S++GHM +RG+V P     Y+EMGAGRG LG A+   +P S+I +++ +  RN  DK L        RA++DIRDL+   LP     QG++  VV VAKHLCGV TDLALRA LTL P         +++ G GG+   D          + S A        S  +A+ G  G     VAIATCCHH  +  DYVG+ F    GF+ RDF A+ R+  W +          P    R P SS      Q                  +   SD                      G+G  G   V  D         A R    R+ K L+D GR  ++ E LG+  ELV+F +R  +PEN LLLA+
Sbjct:   26 PAEGNVKPLRISKKAQRKLAKKDAGQDSQGTGDWDGCRMWVTRKNRYCSQPR-KRDAQYCGLHQ--QEVFDAESRIPCPVDATHSIDKANLQWHLKICTFTKTASDVASQVFYSKGINSGPPLAGSPIAAAADVAAPNGSAFVDPASDPTAADEAPVIDASAVWRMAKASGGDIGGEYLSDLIARIERAYEEHVGSISALQLSPPECEPLKEAADAVKMDLKRMRHIVQQSSIIGHMQRRGMVQP--EHCYIEMGAGRGLLGLALGLTYPDSDILLVDGAKPRNAGDKGLRAKGNPPFRAKVDIRDLDAGKLP---LPQGKR--VVIVAKHLCGVGTDLALRAALTLAPAAALNGQARDHSGGGGGSGASDCGGAXXXXXXSGSRAXXXXXXXXSRFAAAHGLAG-----VAIATCCHHCVNLTDYVGRAFAERCGFDKRDFAAIGRLGGWATTF-------GPEGGGRRPGSSXXXXXAQREGGXXDXXXXXXXXXXNEHSASD---------------------SGIGCEGGGAVRTD---------AERREIGRKSKALIDWGRKEYL-EGLGMEAELVYFCDRAFSPENCLLLAW 571          

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: A0A8K1FML3_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FML3_PYTOL)

HSP 1 Score: 221 bits (562), Expect = 1.820e-61
Identity = 159/455 (34.95%), Postives = 229/455 (50.33%), Query Frame = 3

Query:   45 DSGSVEERERGLSRKRRQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVSENAEFCVTHMPASSF----------ADGNRRVPCPLDPNHNVAERLLESHVKICNKTRQSKELEQQPYFSKDINSGRPSSGK-----------DCDSDVTWDHAGEEAVGEPGNAIGNAAVFDEAFVLDLITRVSAAHREHVGDLSMEVLDPPQVQALHRTVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADKKL-DGVST-------RARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALRAMLTLPPGNIDPIVENTVGEGGNIEKDPVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWIS 1322
            +S + EER     +KR+QEK      A+K+G W+RC FR+  K RFC  +R++  A FC  H+PA +           A+   RVPCP+D +H V +  LE H+K+CNKTR ++  +  PY+S++INSG  +              +   D   +H G  A  E  + I   A  D A   DL+ RV++A+   VG +  E L      AL    T  G      RH+ QQAS++GHM +  L+   +++ ++E+GAGRG L  A+A  FP S   +I+R+  R KAD  + D +S        RA+IDIR LN+AG+  L        P+V ++KHLCGVATDL+LRA            + NTV         P S P                      AG       G+A+A CCHHAC W DY   EF L  GF+A++F  ++ +++W +
Sbjct:   10 ESSATEER-----KKRKQEKL----AAMKSGEWDRCMFRIVRKNRFCNMERIN-GALFCGNHLPADAQVSSKSSQQFKAELRSRVPCPIDGSHTVYQYDLEKHLKVCNKTRDAEAAKALPYYSENINSGTHAVTTREELKAAEVSIEASDDTEKEHDGPTAQQEQ-SLIDKLAALDFA---DLVKRVNSAYDTCVGSIPTEKLRHSCCDALLTEKTAAGASRAALRHIEQQASIIGHMERAKLLD--SKAAFLELGAGRGMLSLALAQTFPDSLYVLIDRAGSRGKADHFIRDNMSDTSAHKVMRAKIDIRHLNLAGMKELV-----DMPIVGMSKHLCGVATDLSLRA------------IANTVPV-------PESHP----------------------AGVISPAFKGIAVALCCHHACQWEDYSNPEFFLAQGFSAQEFPLLKGMTSWAT 402          

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: W4H4G0_9STRA (tRNA:m(4)X modification enzyme TRM13 n=2 Tax=Aphanomyces astaci TaxID=112090 RepID=W4H4G0_9STRA)

HSP 1 Score: 218 bits (554), Expect = 3.140e-60
Identity = 171/585 (29.23%), Postives = 256/585 (43.76%), Query Frame = 3

Query:   93 RQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVSENAEFCVTHMPASSFADGNR----------RVPCPLDPNHNVAERLLESHVKICNKTRQSKELEQQPYFSKDINSGR--------PSSGKDCDSDVTWDHAGEEAVGEPGNAIGNAAVFDEAFVLDL---ITRVSAAHREHVGDLSMEVLDPPQVQALHRTVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADK--KLDGVSTRARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALRAML-TLPPGNIDPIVENTVGEGGNIEKDPVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWISQEPESAWPSAPANLRRNPCSSLEMPQQQSGENGSPAPQQLRRPVNTDRMDSDHCEPMEHYDHGSGHVDRPMPLFGVGGPGQSGVEEDPGVLPAMSG---AARAVAARECKRLLDRGRVWFVQEKLGLTCELVHFVEREVTPENALLLAFRA 1766
            R ++KR +  A+K G W +C F++  K RFC   RV E + +C  H+         +          RVPC LDP H V    L+ H+ ICNK ++++ +   P+++ +INSG         PS+    D+  T +H+G  +  +       A++F     LD      ++ AA  +HV  +  + LD      L +     G      RH+ QQAS++GHM  + L+   +  +YVE+GAGR  L  A+   +P S   +I+R+  R KAD+   LD   TRA+IDIR LN+A +  +       +P+V ++KHLCGVATDL+LRA+  TLP G  D   E   G    +    V                                  G+AIA CCHHACSW DYV   F +++GF A +FK M  +S W +                                                     C                            G+  D G +  + G   A R    R+CKRLLD GRV +++E+ G+  +LVH+ +++ + EN LLLA+R+
Sbjct:   20 RLKRKREKAKAMKDGEWTQCMFKLERKNRFCNVARV-EGSLYCGNHVVDDEGVVSQKTKKYKQELCKRVPCTLDPTHTVYLFDLKKHLVICNKLKEAEVMRSLPFYTANINSGTHAATGTSDPSTTVQDDTSATEEHSGPSSDKQ-------ASLFQTLVGLDFNAFAAKIDAAFDKHVPVIPTQTLDHAACNTLLQQKQAAGANHSILRHIQQQASILGHMESKRLLR--SDCVYVELGAGRAMLSLALTQMYPSSPFVLIDRAGSRGKADQYIALDNKCTRAKIDIRHLNLAKMDQVA-----HQPLVCLSKHLCGVATDLSLRALANTLPAGPSDDATEAPTGHPQKMSSHLV----------------------------------GLAIALCCHHACSWEDYVNPAFFVDMGFTAAEFKLMVPLSGWAT-----------------------------------------------------C----------------------------GMTMDGGAVETVLGFNRADRTRMGRQCKRLLDMGRVHYLRER-GVQAQLVHYCDQDESLENCLLLAWRS 473          

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: A0A024UQA3_9STRA (tRNA:m(4)X modification enzyme TRM13 n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UQA3_9STRA)

HSP 1 Score: 215 bits (548), Expect = 2.290e-59
Identity = 168/581 (28.92%), Postives = 260/581 (44.75%), Query Frame = 3

Query:   93 RQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVSENAEFCVTHMPASSFADGNR----------RVPCPLDPNHNVAERLLESHVKICNKTRQSKELEQQPYFSKDINSGRPSSGKD-----CDSDVTWDHAGEEAVGEPGNAIGNAAVFDEAFVLD---LITRVSAAHREHVGDLSMEVLDPPQVQALHRTVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADKKL--DGVSTRARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALRAML-TLPPGNIDPIVENTVGEGGNIEKDPVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWISQEPESAWPSAPANLRRNPCSSLEMPQQQSGENGSPAPQQLRRPVNTDRMDSDHCEPMEHYDHGSGHVDRPMPLFGVGGPGQSGVEEDPGVLPAMSG---AARAVAARECKRLLDRGRVWFVQEKLGLTCELVHFVEREVTPENALLLAFR 1763
            R ++KR +  A+K G W +C F++  K R C   RV E + +C  H+         +          RVPC LDP+H V    L+ H+ ICNK ++++ +   P++S++INSG  ++G D      D  +  D A  +    P +A   A++F     +D   L  ++ +A+  +V  +  + LD     AL +     G      RH+ QQAS++GHM  + L+ P    +Y E+GAGR  L  A+   +P S   +I+R+  R KAD+ +  +   TRA+IDIR LN+A +  +       +P+V ++KHLCGVATDL+LRA+  TLPP           G  G++   P++                             S   G+AIA CCHHAC W DYV  ++ L++GF   +FK M  +S W +                                                     C                            G+  D G + ++ G   A R    R+CKRLLD GRV +++E+ G+  ELVH+ E++ + EN LLLA++
Sbjct:   21 RLKRKREKSKAMKDGEWTQCMFKLKRKNRLCNVARV-EGSLYCGNHIVDEEGVTSQKTKKHKQELCKRVPCTLDPSHTVYLFDLKKHLVICNKLKEAEAMRSLPFYSENINSGTHAAGSDHVAPPVDEALHVDDANVDEHSCPSSA-KQASLFQTLAAMDFNSLAAKIKSAYDANVPSIPTQKLDHAACTALLKEKQAAGANHSVLRHIQQQASILGHMEAKHLLRPS--HVYCELGAGRAMLSLALTQIYPSSPFVLIDRAGSRGKADQHIATENKCTRAKIDIRHLNLAKMEQVAN-----QPLVCLSKHLCGVATDLSLRALANTLPPSE---------GSAGSVPP-PIT-----------------------------SHFVGLAIALCCHHACRWEDYVHPQYFLDMGFTVAEFKLMVSLSGWAT-----------------------------------------------------C----------------------------GMSTDGGAVESVLGLNRADRTQLGRQCKRLLDMGRVHYLRER-GVRAELVHYCEQDESLENCLLLAWQ 471          

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: A0A5D6XLD7_9STRA (tRNA:m(4)X modification enzyme TRM13 n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XLD7_9STRA)

HSP 1 Score: 214 bits (545), Expect = 1.170e-58
Identity = 156/450 (34.67%), Postives = 229/450 (50.89%), Query Frame = 3

Query:   93 RQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVSENAEFCVTHMP-----ASSFA-----DGNRRVPCPLDPNHNVAERLLESHVKICNKTRQSKELEQQPYFSKDINSG---------RPSSGKDCDSDVTWDHAGEEAVGEPGNAIGNA----AVFDEAFVLD---------LITRVSAAHREHVGDLSMEVLDPPQVQALHRTVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADKKLDGVST-------RARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALRAML-TLPPGNIDPIVENTVGEGGNIEKDPVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWIS 1322
            ++++K+ +  A+++G WERC FR+  K+RFC   R++  A FC  H+P     AS+ A     D  +RVPCP+D  H V    L+ HV +CNK + +  +   PY+ ++INSG          P +    D+       G +   EP  A   A    +  DE  +LD         L  RV AA+   VG++ +E L     +AL     D G      RH+ QQAS++GHM +  L+     S++VE+GAGRG L  A+A +F  S   +++R+  R KAD+ ++G          RA+IDIR LN+AG+  +       RPVV ++KHLCGVATDL+LRA+  TLP G                       PG      A+  + + +  VSA A +   R  G+A+A CCHH C+W DYV  EF    GF AR+F  +  +S+W +
Sbjct:   24 KRQRKKLKHAAMQSGDWERCMFRLVRKDRFCNMARIN-GALFCGNHLPPDADVASNKAGRYKTDRRQRVPCPVDKRHTVYVYDLDKHVLVCNKVKDAAAMTLLPYYVENINSGAHCVRKAGSEPPAAPIADATPEAPPIGADGGEEPKEAAAAAHAGLSANDEQGLLDKLLAVDFPDLSRRVHAAYERCVGEIPLEKLTHACCEALFAEKKDAGAAKNVLRHIEQQASILGHMARVNLLD--ASSVFVELGAGRGMLSLALAQSFRDSVFVLVDRAHTRGKADRFIEGPDEAPSRKVLRAKIDIRHLNLAGMQEVV-----DRPVVCMSKHLCGVATDLSLRAVTQTLPRG-----------------------PG------AESAASSAQPAVSALAVSPLFR--GLAVALCCHHVCAWEDYVNPEFFEAHGFLAREFPLLTGMSSWAT 434          

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: A0A485KSY0_9STRA (tRNA:m(4)X modification enzyme TRM13 n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KSY0_9STRA)

HSP 1 Score: 212 bits (539), Expect = 4.380e-58
Identity = 171/585 (29.23%), Postives = 251/585 (42.91%), Query Frame = 3

Query:   93 RQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVSENAEFCVTHMPASSF----------ADGNRRVPCPLDPNHNVAERLLESHVKICNKTRQSKELEQQPYFSKDINSGRPSSGKDCDSDVTWDH-------AGEEAVGEPGNAIGNAAVFDEAFVLD---LITRVSAAHREHVGDLSMEVLDPPQVQALHRTVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADKKL--DGVSTRARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALRAML-TLPPGNIDPIVENTVGEGGNIEKDPVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWISQEPESAWPSAPANLRRNPCSSLEMPQQQSGENGSPAPQQLRRPVNTDRMDSDHCEPMEHYDHGSGHVDRPMPLFGVGGPGQSGVEEDPGVLPAMSGAARAVAARECKRLLDRGRVWFVQEKLGLTCELVHFVEREVTPENALLLAFRA*ETA 1778
            R ++KR +  A+K G W  C F++  K R+C   RV E +++C  H+               A+  +RVPC LDP+H V    L+ H++ICNK ++++ +   PY+S +INSG  +                    + ++      +    A +F     +D   L  +++AA   HV  +  + L       L       G      RH+ QQAS++GHM  + L+ P    +YVE+GAGR  L  A+   +P S   +I+R+  R KAD+ +  +   TRA+IDIR LN+ G+  +      Q+PVV ++KHLCGVATDL+LRA+  TLPP   D +  + VG                                             +AIA CCHHAC W DYV Q F LE+GF   DF  M  +S W +                                                     C                    G+G  G + VE     +   S AAR    R+CKRL+D GRV +++  LGL  ELVH+ ++  + EN LLLA+R  + A
Sbjct:   25 RNKRKREKAKAMKDGEWTHCMFKLERKNRYCNVARV-EGSKYCGNHIVDEEGVVSQKTKKYKAEMRKRVPCTLDPSHTVYLFDLKKHLEICNKLKEAEAMRSLPYYSLNINSGTHTGASTXXXXXXXXXXXXXXXXSTDDQEHSAPSLEKQANLFQTLAKMDFNALAKKIAAAFDAHVSAIPTQTLQHRACDILLEQKQASGAAHSVLRHIQQQASILGHMDAKHLLHPD--HVYVELGAGRAMLSLALTQIYPASPYVLIDRAGSRGKADQYIATENTCTRAKIDIRHLNLGGMDQVV-----QKPVVCLSKHLCGVATDLSLRALANTLPPVAADTMSPHLVG---------------------------------------------LAIALCCHHACCWDDYVNQAFFLEMGFTRADFALMVPLSGWAT-----------------------------------------------------C--------------------GMGTEGDA-VE----TVLGWSRAARTALGRQCKRLMDMGRVLYLRH-LGLHAELVHYCDQAESLENCLLLAWRQDDAA 477          

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: A0A225W5E4_9STRA (tRNA:m(4)X modification enzyme TRM13 n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225W5E4_9STRA)

HSP 1 Score: 211 bits (536), Expect = 1.100e-57
Identity = 152/452 (33.63%), Postives = 221/452 (48.89%), Query Frame = 3

Query:   54 SVEERERGLSRKRRQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVSENAEFCVTHMPASSFADGN----------RRVPCPLDPNHNVAERLLESHVKICNKTRQSKELEQQPYFSKDINSGRPSSGKDCDSDVT------WDHAGEEAVGEPGNAIGNAAVFDEAFVLDLITRVSAAHREHVGDLSMEVLDPPQVQALHRTVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADKKLDG------------VSTRARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALRAML-TLPPGNIDPIVENTVGEGGNIEKDPVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWIS 1322
            S E+  R + RKRR   K+A+  A   G W+RC F+V  K R+C   RVS +  FC  H+P S  +             RRVPCP+D +H V E  L  HV +CN+ + +  +++ PY+S +INSG   S K   S          +H+   A  + G  I      D A   DL  R+  A+   VG+L++E L       L       G      RH+ QQAS++GHM K  L+   + + +VE+GAGRG L  A+A  +P S   +I+R+  R KAD+ + G             + RA+IDIR LN AG+  +       +PVV ++KHLCGVATDL+LRA+  TLP  NI+ +                               +++ ++ S S+   G     +AIA CCHH C+W DYV   F L  GF A +F  +  +++W +
Sbjct:    6 SAEQLSRDVQRKRR---KQAKAEAKDGGEWDRCMFKVVRKNRYCNISRVSGSL-FCGNHLPDSDASKSKKSQKFKAATRRRVPCPVDSSHTVYEYDLAKHVLVCNRVKDADTMKKLPYYSHNINSGTHCSDKTESSSTEEXXXXXXEHSAPSAQEQQG-IIDKLQAIDFA---DLRKRIDTAYAACVGELTLEKLHHKCCDQLFEEKKKAGASKSVLRHIEQQASIIGHMEKATLL-EDSNAAFVELGAGRGMLSLALAQMYPDSLYVLIDRAHTRGKADRFIGGDGKDDEAAKENSTTLRAKIDIRHLNFAGMQEILN-----KPVVCMSKHLCGVATDLSLRAIAQTLPEPNINVV-------------------------------QSIDTSTSVSSSFQG-----LAIALCCHHVCAWEDYVNPAFFLAQGFTAEEFVLLTSMTSWTT 407          

BLAST of mRNA_H-paniculata_contig1053.348.1 vs. uniprot
Match: A0A067CWT3_SAPPC (tRNA:m(4)X modification enzyme TRM13 n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067CWT3_SAPPC)

HSP 1 Score: 209 bits (531), Expect = 6.140e-57
Identity = 141/427 (33.02%), Postives = 211/427 (49.41%), Query Frame = 3

Query:   93 RQEKKRAQQLALKTGLWERCRFRVPGKERFCAQQRVS---------ENAEFCVTHMPASSFADGNRRVPCPLDPNHNVAERLLESHVKICNKTRQSKELEQQPYFSKDINSGRPSSGKDCDSDVTWDHAG---EEAVGEPGNAIGNAAVFDEAFVLD---LITRVSAAHREHVGDLSMEVLDPPQVQALHRTVTDGGVGFRKGRHVLQQASLVGHMLKRGLVGPGTRSLYVEMGAGRGALGQAIATAFPGSEITMIERSSIRNKADKKL--DGVSTRARIDIRDLNMAGLPSLTTGQGEQRPVVAVAKHLCGVATDLALRAMLTLPPGNIDPIVENTVGEGGNIEKDPVSKPGRSARSAADLKSETVRSTVSASAGTYGSRAGGVAIATCCHHACSWRDYVGQEFLLELGFNARDFKAMQRISAWIS 1322
            R ++KR +  A   G W +C F++  K R+C   RV+         E  E  V++      A+  +RVPC LDP+H V    L+ H+ +CNK ++++ ++  PYFS++INSG  ++    ++DV+ D      EE   E  +A   A++F     +D      +V AA+ + VG +  E L       L       G      RH+ QQAS++GHM ++ L+       Y+E+GAGR  L  A+   +P S   +I+R+  R KAD+ +  +   TRA+IDIR LN+AG+   T     + P+V ++KHLCGVATDL+LRA+ T  P    P+  N +                                             G+AIA CCHHACSW DYV   +L ELGF+A DFK +  +S W +
Sbjct:   30 RSKRKRDKFKAAADGEWTQCMFKLERKARYCNVARVAGSLYCGNHIERDEGVVSNKSKKFKAEMRQRVPCTLDPSHTVYLYDLKKHLVVCNKLKEAEAMQALPYFSRNINSGSHAAAPQDNTDVSVDEPDNETEENSHEAPSAARQASIFQILAKMDYTAFAAKVEAAYAKAVGSIPTETLKHDACTKLLVEKEATGASHGALRHIQQQASIIGHMERKSLLHKSFT--YMELGAGRAMLSLALTQLYPASPFILIDRAGTRGKADQYIATESTCTRAKIDIRHLNLAGMKEAT-----ETPLVCLSKHLCGVATDLSLRALATTLP----PVQANAMSP----------------------------------------HLHGLAIALCCHHACSWDDYVNPTYLEELGFSAADFKVLVSLSGWAT 405