mRNA_H-paniculata_contig14440.2669.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig14440.2669.1
Unique NamemRNA_H-paniculata_contig14440.2669.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5JN68_9PHAE (DDE-1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JN68_9PHAE)

HSP 1 Score: 119 bits (297), Expect = 5.940e-26
Identity = 76/162 (46.91%), Postives = 97/162 (59.88%), Query Frame = 1
Query:  367 INHSRQLSGGVGRREALNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFRKRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKVRAT-TKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM---QSSTRCRWRPRWRR 840
            IN  R+   G G RE  +KL +L PDALGG+P++    +   F  KF   Y +FRKRN FSI  RT+V QKLP+GYEG +W  V+K+R T  K A EIYA +     P A D+S      ++  +L+SEQ +SV+ E  R        QSSTRCRWRP WRR
Sbjct:  120 INEQRKQHRGCGNREVTSKLLELKPDALGGMPANTKPEEALAFKVKFNSWYQRFRKRNGFSIRRRTSVGQKLPKGYEGMAWTTVMKLRETLVKRAGEIYARRQ----PPAPDESP-----IKGEDLTSEQLVSVEAEDSRNSATWIRRQSSTRCRWRPLWRR 272          
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5JTG5_9PHAE (DDE-1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTG5_9PHAE)

HSP 1 Score: 114 bits (285), Expect = 2.130e-24
Identity = 71/145 (48.97%), Postives = 90/145 (62.07%), Query Frame = 1
Query:  367 INHSRQLSGGVGRREALNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFRKRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKVR-ATTKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM 798
            IN  R+L  G G RE +NKL +L PDALGG+P++    +   F  KF   Y +FRKRN FSI  R +V QKLP+GYEG +WA V K+R A  K A EIYA +     P A D+S      ++  +L+SEQ  SV+ EVF ELGNM
Sbjct:  139 INEQRKLHRGCGNREVMNKLLELKPDALGGMPANAKPEEALAFKVKFNSWYQRFRKRNGFSIRRRASVGQKLPKGYEGMAWATVTKLREALVKRAGEIYARRH----PPAPDESP-----IKGEDLTSEQPASVEAEVFEELGNM 274          
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5KFQ9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KFQ9_9PHAE)

HSP 1 Score: 110 bits (274), Expect = 6.390e-24
Identity = 76/172 (44.19%), Postives = 100/172 (58.14%), Query Frame = 1
Query:  286 SRAWKVPAAFGAITEGARPAGLTWRRRINHSRQLSGGVGRREALNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFRKRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKVR-ATTKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM 798
            S+A +VP+   A T  A    L     IN  R+   G G RE +NKL QL PDALGG+P++    +   F  KF   Y QFRKRN FSI  RT+V QKLP+GYEG +WA V+ +R A  + A  I+A ++    P A D+S      ++  +L+ EQ  SV+ EVF ELGNM
Sbjct:  146 SKAKQVPSGGKASTVDAAEQALV--DYINEQRKQHRGCGNREVINKLLQLKPDALGGMPANAKPEEALAFKIKFNSWYQQFRKRNGFSIRRRTSVGQKLPKGYEGTAWATVMNLREALVQRAGAIHARRN----PPAPDESP-----IKGEDLTCEQLASVEAEVFEELGNM 306          
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5KJN9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KJN9_9PHAE)

HSP 1 Score: 112 bits (279), Expect = 7.220e-24
Identity = 70/145 (48.28%), Postives = 90/145 (62.07%), Query Frame = 1
Query:  367 INHSRQLSGGVGRREALNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFRKRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKVR-ATTKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM 798
            IN  R+   G G RE +NKL +L PDALGG+P++    +   F   F   Y +FRKRN FSI  RT+V QKLP+GYEG +WA V+K+R A  K A EIYA +     P A D+S      ++  +L+SEQ  SV+ EVF ELGNM
Sbjct:  108 INEQRKQHRGCGNREVMNKLLELKPDALGGMPANAKPEEALAFKVNFNPWYQRFRKRNGFSIRRRTSVGQKLPKGYEGMAWATVMKLREALVKRAGEIYARRH----PPAPDESP-----IKGEDLTSEQLASVKAEVFEELGNM 243          
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5K7Y1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K7Y1_9PHAE)

HSP 1 Score: 110 bits (274), Expect = 3.780e-23
Identity = 74/147 (50.34%), Postives = 93/147 (63.27%), Query Frame = 1
Query:  415 LNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFR-KRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKVR-ATTKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM---QSSTRCRWRPRWRR 840
            +NKL +L PDALGG+P++V   +   F  KF   Y +FR KR  FSI  RT+V QKL +GYEG +WA  +K+R A  K A EIYA +     P A D+S      ++  +L+SEQ  SV  EVF ELGNM   Q+STRCRWRP WRR
Sbjct:    1 MNKLLELKPDALGGMPANVKPEEALAFKVKFNSWYQRFRSKRYGFSIRRRTSVGQKLSKGYEGMAWATGMKLREALVKRAGEIYARRH----PPAPDESP-----IKGEDLTSEQLASVGAEVFEELGNMDRRQTSTRCRWRPLWRR 138          
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5KKK4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KKK4_9PHAE)

HSP 1 Score: 105 bits (262), Expect = 7.800e-23
Identity = 66/145 (45.52%), Postives = 90/145 (62.07%), Query Frame = 1
Query:  367 INHSRQLSGGVGRREALNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFRKRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKVRAT-TKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM 798
            IN  R+     G RE ++KL ++ PDALGG+P++    +      KF   Y +FRKRN FSI  RT+V QKLP+G+EG +WA V+K+R T +K A EIYA +     P A D+S      ++  +L+SEQ  SV+ EVF ELGNM
Sbjct:   82 INDQRKQPRRCGNREVMDKLLEMKPDALGGMPANAKPEEALALKVKFNSWYQRFRKRNGFSIRRRTSVGQKLPKGHEGMAWATVMKLRETLSKRAGEIYARRH----PPAPDESP-----IKGVDLTSEQLASVEAEVFEELGNM 217          
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5JAU0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JAU0_9PHAE)

HSP 1 Score: 108 bits (271), Expect = 9.920e-23
Identity = 69/145 (47.59%), Postives = 90/145 (62.07%), Query Frame = 1
Query:  367 INHSRQLSGGVGRREALNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFRKRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKVR-ATTKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM 798
            IN  R+   G G RE +NKL +L PDALGG+P++    +   F  KF   Y +FRKR+  SI  RT+V QKLP+GYEG +WA V+K+R A  K A EIYA +     P A D+S +     +  +L+SEQ  SV+ EVF ELGNM
Sbjct:  153 INEQRKQHRGCGNREVMNKLLELKPDALGGMPANAKPEEALAFKVKFNSWYQRFRKRHGSSIRQRTSVGQKLPKGYEGMAWATVMKLREALVKRAGEIYARRH----PPAPDESPT-----KGEDLTSEQLASVEAEVFEELGNM 288          
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5JCV1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JCV1_9PHAE)

HSP 1 Score: 107 bits (267), Expect = 3.690e-22
Identity = 68/145 (46.90%), Postives = 90/145 (62.07%), Query Frame = 1
Query:  367 INHSRQLSGGVGRREALNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFRKRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKVR-ATTKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM 798
            IN  R+   G G RE +NK+ +L PDALGG+P++    +   F  KF   Y +FRKR+  SI  RT+V QKLP+GYEG +WA V+K+R A  K A EIYA +     P A D+S +     +  +L+SEQ  SV+ EVF ELGNM
Sbjct:  207 INEQRKQHRGCGNREVMNKVLELKPDALGGMPANAKPEEALAFKVKFNSWYQRFRKRHGSSIRRRTSVGQKLPKGYEGMAWATVMKLREALVKRAGEIYARRH----PPAPDESPT-----KGEDLTSEQLASVEAEVFEELGNM 342          
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5L819_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L819_9PHAE)

HSP 1 Score: 105 bits (262), Expect = 6.030e-22
Identity = 67/145 (46.21%), Postives = 88/145 (60.69%), Query Frame = 1
Query:  367 INHSRQLSGGVGRREALNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFRKRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKVR-ATTKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM 798
            IN   +   G G RE +NKL +L PDALGG+P++    +   F  KF   Y +FRKRN F I  RT+V QKLP+GYEG +WA V+K+R A  K A EIYA +     P A D+S      ++  +L+SEQ  SV+ EVF E+G M
Sbjct:  136 INERHKQHRGCGNREVMNKLLELKPDALGGVPANAKPEEALAFKVKFNSWYQRFRKRNGFRIRRRTSVGQKLPKGYEGMAWATVMKLREALVKRAGEIYARRH----PPAHDESP-----IKGEDLTSEQLASVEAEVFEEVGIM 271          
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Match: A0A6H5JHT8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHT8_9PHAE)

HSP 1 Score: 106 bits (265), Expect = 1.020e-21
Identity = 65/135 (48.15%), Postives = 86/135 (63.70%), Query Frame = 1
Query:  397 VGRREALNKLCQL*PDALGGLPSSVNAAKIEQFNSKFGELYAQFRKRNRFSIPPRTTVAQKLPQGYEGKSWAWVLKV-RATTKWAAEIYAVKDSVAAPSA*DQSASAALNVEATELSSEQQLSVQEEVFRELGNM 798
            +G RE +NKL +L PDALGG+P++    +   F  KF   Y +FRKRN FSI  RT+V QKLP+GYEG +WA V+K+  A  K A EIYA +     P A D+S      ++  +L+SEQ  SV+ EVF E+GNM
Sbjct:  189 LGNREVMNKLLELKPDALGGMPANAKPEEAIDFKFKFNSWYQRFRKRNGFSIRRRTSVGQKLPKGYEGMAWATVMKLCEALVKRAGEIYARRH----PPAPDESP-----IKGEDLNSEQLASVEAEVFEEVGNM 314          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig14440.2669.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JN68_9PHAE5.940e-2646.91DDE-1 domain-containing protein n=1 Tax=Ectocarpus... [more]
A0A6H5JTG5_9PHAE2.130e-2448.97DDE-1 domain-containing protein n=1 Tax=Ectocarpus... [more]
A0A6H5KFQ9_9PHAE6.390e-2444.19Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5KJN9_9PHAE7.220e-2448.28Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5K7Y1_9PHAE3.780e-2350.34Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5KKK4_9PHAE7.800e-2345.52Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JAU0_9PHAE9.920e-2347.59Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JCV1_9PHAE3.690e-2246.90Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5L819_9PHAE6.030e-2246.21Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JHT8_9PHAE1.020e-2148.15Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig14440contigH-paniculata_contig14440:1859..3219 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons2
Model size1018
Cds size192
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig14440.2669.1prot_H-paniculata_contig14440.2669.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig14440 1873..2407 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929149.2527463-UTR-H-paniculata_contig14440:1858..18721622929149.2527463-UTR-H-paniculata_contig14440:1858..1872Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig14440 1859..1872 -
1691480750.2912776-UTR-H-paniculata_contig14440:1858..18721691480750.2912776-UTR-H-paniculata_contig14440:1858..1872Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig14440 1859..1872 -
1622929149.2903829-UTR-H-paniculata_contig14440:2407..32191622929149.2903829-UTR-H-paniculata_contig14440:2407..3219Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig14440 2408..3219 -
1691480750.3225641-UTR-H-paniculata_contig14440:2407..32191691480750.3225641-UTR-H-paniculata_contig14440:2407..3219Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig14440 2408..3219 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929149.2623444-CDS-H-paniculata_contig14440:1872..20031622929149.2623444-CDS-H-paniculata_contig14440:1872..2003Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14440 1873..2003 -
1691480750.303559-CDS-H-paniculata_contig14440:1872..20031691480750.303559-CDS-H-paniculata_contig14440:1872..2003Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14440 1873..2003 -
1622929149.2789218-CDS-H-paniculata_contig14440:2346..24071622929149.2789218-CDS-H-paniculata_contig14440:2346..2407Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14440 2347..2407 -
1691480750.3139138-CDS-H-paniculata_contig14440:2346..24071691480750.3139138-CDS-H-paniculata_contig14440:2346..2407Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig14440 2347..2407 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig14440.2669.1

>prot_H-paniculata_contig14440.2669.1 ID=prot_H-paniculata_contig14440.2669.1|Name=mRNA_H-paniculata_contig14440.2669.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=64bp
MPVETTLETTRVQDARISTARKQKERFTLHLAMMSDGRNVTPRIAVKGTL
LFLGDESCRRQPM*
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mRNA from alignment at H-paniculata_contig14440:1859..3219-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig14440.2669.1 ID=mRNA_H-paniculata_contig14440.2669.1|Name=mRNA_H-paniculata_contig14440.2669.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=1361bp|location=Sequence derived from alignment at H-paniculata_contig14440:1859..3219- (Halopteris paniculata Hal_grac_a_UBK monoicous)
GGCAACACCACAGAGGGCACGAGCAGCACAGGCGGCGGTCCCTCCAACGA TACGAATTAGAATAAAGAGTGAGCTCCGTAAAAATCTGCCCATGATCCCA GCGTTGGGACTGGTGGTACGCCCCAGAGACCTGCTGATTCTGCGGCTGTA GGTGTTGCTAACCCCAAGGCAGTAGTGGAGACAATTGATGCAACACAGGC AACACGGGCCCGGCAAGAGTCCTTCGTGTGCACAAGAAGATAATTATCGC GTGGTGCAAGAGCGAAAAACGGCTGAAGAGGCCAGTCAAGAGCCTGGAAG GTGCCCGCAGCCTTCGGGGCAATAACAGAGGGCGCCCGACCAGCAGGGCT GACGTGGAGAAGGAGGATCAACCACAGTCGTCAACTATCCGGCGGCGTCG GTAGGCGGGAAGCCTTGAACAAGCTCTGTCAGCTTTAGCCCGACGCCCTT GGCGGACTGCCGAGCAGTGTTAATGCTGCCAAGATAGAACAGTTCAACAG CAAGTTCGGCGAATTGTACGCCCAGTTTCGCAAGAGGAACAGGTTTTCCA TCCCTCCTCGTACCACCGTCGCTCAAAAGCTCCCGCAGGGATATGAGGGA AAGTCATGGGCGTGGGTGCTGAAAGTTCGCGCCACCACGAAATGGGCCGC TGAGATCTACGCCGTCAAGGATTCAGTAGCCGCGCCGAGCGCATAGGATC AGAGCGCGAGTGCTGCTCTGAATGTGGAAGCGACGGAGCTATCCTCTGAG CAGCAGTTATCGGTACAGGAGGAAGTGTTCCGTGAGCTGGGGAACATGCA ATCCAGCACGAGATGCCGGTGGAGACCACGCTGGAGACGACTAGAGTGCA GGACGCGCGTATTTCTACGGCACGTAAGGCCGTCGAACATTCGTACAGGA CAGTACCTGTGGTAAAATTGGCACTGACATTGCAGAAAACGCTCATGTCT GTAACTTGACGGTTTGTATTTTGTTTTCTTGCATTTGATATACGGTGGTA GAGTCAGTGCTCGGTACACTGTCCAACATAGTACACTGTGCTATGCTGAC TGTACACTCCTGGTTGTACTGTGTTTTTCTTTTTGTGATGCACCGACTGT GAGAGTGCACTGGCTATATCTGTGTCATATTTCAGATACAAGTCTACCGA CCATACGCGATCCCAATAAAGTTATTTCACCATGCTCCATTCCTGCTGCT TTCTCGACCTCCGTAGGCAAGCAGAAGGAGCGCTTTACACTTCATCTCGC TATGATGAGTGATGGACGGAACGTGACTCCGCGCATCGCTGTTAAGGGCA CGCTGCTCTTCTTGGGAGATGAATCCTGTCGACGGCAGCCCATGTAGGCA CCCCACGAACG
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Coding sequence (CDS) from alignment at H-paniculata_contig14440:1859..3219-

>mRNA_H-paniculata_contig14440.2669.1 ID=mRNA_H-paniculata_contig14440.2669.1|Name=mRNA_H-paniculata_contig14440.2669.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=384bp|location=Sequence derived from alignment at H-paniculata_contig14440:1859..3219- (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGCCGGTGGAGACCACGCTGGAGACGACTAGAGTGCAGGACGCGCGTAT
TTCTACGGCACATGCCGGTGGAGACCACGCTGGAGACGACTAGAGTGCAG
GACGCGCGTATTTCTACGGCACGCAAGCAGAAGGAGCGCTTTACACTTCA
TCTCGCTATGATGAGTGATGGACGGAACGTGACTCCGCGCATCGCTGTTA
AGGGCACGCTGCTCTTCTTGGGAGATGAATCCTGTCGACGGCAGCCCATG
TAGGCAAGCAGAAGGAGCGCTTTACACTTCATCTCGCTATGATGAGTGAT
GGACGGAACGTGACTCCGCGCATCGCTGTTAAGGGCACGCTGCTCTTCTT
GGGAGATGAATCCTGTCGACGGCAGCCCATGTAG
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