mRNA_H-paniculata_contig3252.8746.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig3252.8746.1
Unique NamemRNA_H-paniculata_contig3252.8746.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: D7FVG1_ECTSI (Molybdopterin biosynthesis protein MoeB n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVG1_ECTSI)

HSP 1 Score: 187 bits (476), Expect = 3.950e-56
Identity = 94/103 (91.26%), Postives = 101/103 (98.06%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LPELN DEVRRYSRHLILPDVGV+GQR LKASSVLAVGTGGLGSPALLYLAAAGVGRLG++DDDVVDESNLQRQVIHGT+TLGKPKV+SARDRMLDVNPH++V
Sbjct:   20 LPELNQDEVRRYSRHLILPDVGVKGQRRLKASSVLAVGTGGLGSPALLYLAAAGVGRLGIVDDDVVDESNLQRQVIHGTATLGKPKVDSARDRMLDVNPHIKV 122
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: A0A6H5L9U9_9PHAE (Rhodanese domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L9U9_9PHAE)

HSP 1 Score: 187 bits (476), Expect = 4.790e-55
Identity = 94/103 (91.26%), Postives = 101/103 (98.06%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LPELN DEVRRYSRHLILPDVGV+GQR LKASSVLAVGTGGLGSPALLYLAAAGVGRLG++DDDVVDESNLQRQVIHGT+TLGKPKV+SARDRMLDVNPH++V
Sbjct:  142 LPELNQDEVRRYSRHLILPDVGVKGQRRLKASSVLAVGTGGLGSPALLYLAAAGVGRLGIVDDDVVDESNLQRQVIHGTATLGKPKVDSARDRMLDVNPHIKV 244
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: A0A836CQ68_9STRA (Molybdopterin biosynthesis protein MoeB n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CQ68_9STRA)

HSP 1 Score: 172 bits (435), Expect = 1.320e-49
Identity = 87/103 (84.47%), Postives = 94/103 (91.26%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LP L  DEVRRYSRHLILPDVGV GQR LKASSVLA+G GGLGSPALLYLAAAGVGR+G++DDDVVDESNLQRQVIHGTSTLG  KVESAR R+LD+NPHV+V
Sbjct:   73 LPHLTQDEVRRYSRHLILPDVGVEGQRRLKASSVLAIGAGGLGSPALLYLAAAGVGRVGIVDDDVVDESNLQRQVIHGTSTLGVSKVESARTRILDINPHVQV 175
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: A0A7S2XVR8_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XVR8_9STRA)

HSP 1 Score: 156 bits (395), Expect = 6.210e-46
Identity = 75/103 (72.82%), Postives = 91/103 (88.35%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LP L ++E+ RY+RHLILPD GV GQR LKASSVL +GTGGLGSP LLYLAAAG+GRLG++DDD+VDESNLQRQ+IHGTS +GKPKV+SA  R+ ++NPHV+V
Sbjct:   57 LPVLTNEEIPRYARHLILPDCGVEGQRKLKASSVLCIGTGGLGSPLLLYLAAAGIGRLGIIDDDIVDESNLQRQIIHGTSQVGKPKVQSASARINEINPHVKV 159
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: A0A2E8RYB4_9DELT (Molybdenum cofactor biosynthesis protein MoeB n=1 Tax=Myxococcales bacterium TaxID=2026763 RepID=A0A2E8RYB4_9DELT)

HSP 1 Score: 160 bits (404), Expect = 8.940e-46
Identity = 80/103 (77.67%), Postives = 92/103 (89.32%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LPEL+ +E+ RYSRHLIL +VG+ GQRTLK SSVL VGTGGLGSP LLYLAAAGVGRLG++D DVVDESNLQRQV+HGT+TLG  KVESARDR+ D+NPHV+V
Sbjct:    3 LPELSREEISRYSRHLILDEVGMDGQRTLKGSSVLCVGTGGLGSPLLLYLAAAGVGRLGIVDFDVVDESNLQRQVVHGTATLGVKKVESARDRLKDINPHVQV 105
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: A0A7Y4RHI1_9CHLR (Molybdopterin-synthase adenylyltransferase MoeB n=1 Tax=Anaerolineales bacterium TaxID=2073117 RepID=A0A7Y4RHI1_9CHLR)

HSP 1 Score: 155 bits (393), Expect = 4.500e-44
Identity = 74/103 (71.84%), Postives = 91/103 (88.35%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LPELNH+E+ RYSRHL++P+VG+ GQR LK SS L +GTGGLGSP  LYLAAAGVGR+G++D DVVD SNLQRQVIHGTST+GK KVESARD++LD+NP +++
Sbjct:    2 LPELNHEEILRYSRHLLIPEVGLEGQRKLKNSSALVIGTGGLGSPVALYLAAAGVGRIGLVDYDVVDSSNLQRQVIHGTSTIGKLKVESARDKLLDLNPDIQI 104
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: A0A7S0ITP1_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Calcidiscus leptoporus TaxID=127549 RepID=A0A7S0ITP1_9EUKA)

HSP 1 Score: 148 bits (374), Expect = 8.490e-44
Identity = 71/103 (68.93%), Postives = 87/103 (84.47%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LPEL+H+E+RRYSRHLIL DVG+ GQR LK +SVL +G+GGLGSP L+YLAAAGVG LG++DDDVVDESNLQRQ++H T  +  PKV+SA  R+ D+NPHV V
Sbjct:   37 LPELSHEEIRRYSRHLILDDVGMEGQRRLKGASVLCIGSGGLGSPVLMYLAAAGVGVLGLVDDDVVDESNLQRQIVHSTGRIDTPKVDSASARVADINPHVTV 139
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: A0A661W8B3_9CHLR (Molybdenum cofactor biosynthesis protein MoeB (Fragment) n=1 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A661W8B3_9CHLR)

HSP 1 Score: 149 bits (377), Expect = 1.110e-43
Identity = 74/103 (71.84%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LPEL+H E+ RYSRHL++PDVG+ GQ+ LKA+SVL VGTGGLGSP  LYLAAAGVGR+G++D DVVD SNLQRQVIHG S LG  KV SAR+RMLD+NP ++V
Sbjct:    2 LPELSHKEIHRYSRHLLIPDVGLEGQKKLKAASVLIVGTGGLGSPVALYLAAAGVGRIGLVDYDVVDFSNLQRQVIHGESRLGDLKVHSARERMLDINPEIQV 104
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: A0A2V7SCU0_9BACT (Molybdenum cofactor biosynthesis protein MoeB n=2 Tax=unclassified Gemmatimonadetes TaxID=234665 RepID=A0A2V7SCU0_9BACT)

HSP 1 Score: 156 bits (395), Expect = 1.320e-43
Identity = 76/103 (73.79%), Postives = 90/103 (87.38%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LPEL+H+E+ RYSRHLILPDVGV GQR LKA+ VL VG GGLGSPA LYLAAAGVG LG++D DVVD +NLQRQ++HGTS LG+PK+ESAR+R+ D+NPHV V
Sbjct:  100 LPELSHEEILRYSRHLILPDVGVEGQRKLKAARVLLVGAGGLGSPAALYLAAAGVGTLGIVDFDVVDRTNLQRQILHGTSQLGRPKLESARERLHDINPHVRV 202
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BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Match: A0A0M0JTP2_9EUKA (Adenylyltransferase sulfurtransferase n=1 Tax=Chrysochromulina tobinii TaxID=1460289 RepID=A0A0M0JTP2_9EUKA)

HSP 1 Score: 152 bits (384), Expect = 1.930e-43
Identity = 75/103 (72.82%), Postives = 90/103 (87.38%), Query Frame = 1 
Query:    1 LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYLAAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPHVEV 309
            LPEL+H+E+RRYSRHLIL D G+RGQR LKA+ VL +G+GGLGSP L+YLAAAGVG LG++DDDVVDESNLQRQ+IH TSTLGK KV+SA  R+ D+NP+V V
Sbjct:   14 LPELSHEEIRRYSRHLILDDFGMRGQRKLKATKVLCIGSGGLGSPVLMYLAAAGVGTLGLVDDDVVDESNLQRQIIHTTSTLGKSKVDSAAARVTDINPNVVV 116
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig3252.8746.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FVG1_ECTSI3.950e-5691.26Molybdopterin biosynthesis protein MoeB n=1 Tax=Ec... [more]
A0A6H5L9U9_9PHAE4.790e-5591.26Rhodanese domain-containing protein n=1 Tax=Ectoca... [more]
A0A836CQ68_9STRA1.320e-4984.47Molybdopterin biosynthesis protein MoeB n=1 Tax=Tr... [more]
A0A7S2XVR8_9STRA6.210e-4672.82Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
A0A2E8RYB4_9DELT8.940e-4677.67Molybdenum cofactor biosynthesis protein MoeB n=1 ... [more]
A0A7Y4RHI1_9CHLR4.500e-4471.84Molybdopterin-synthase adenylyltransferase MoeB n=... [more]
A0A7S0ITP1_9EUKA8.490e-4468.93Hypothetical protein (Fragment) n=1 Tax=Calcidiscu... [more]
A0A661W8B3_9CHLR1.110e-4371.84Molybdenum cofactor biosynthesis protein MoeB (Fra... [more]
A0A2V7SCU0_9BACT1.320e-4373.79Molybdenum cofactor biosynthesis protein MoeB n=2 ... [more]
A0A0M0JTP2_9EUKA1.930e-4372.82Adenylyltransferase sulfurtransferase n=1 Tax=Chry... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig3252contigH-paniculata_contig3252:1353..2565 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score189.9
Seed ortholog evalue6e-46
Seed eggNOG ortholog2880.D7FVG1
Preferred namecnxF
KEGG rclassRC00043
KEGG koko:K11996,ko:K18469
KEGG ReactionR07459,R07461
KEGG Pathwayko04122,map04122
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0001403,GO:0001558,GO:0002097,GO:0002098,GO:0002143,GO:0003674,GO:0003824,GO:0004792,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006139,GO:0006163,GO:0006396,GO:0006399,GO:0006400,GO:0006464,GO:0006725,GO:0006732,GO:0006753,GO:0006777,GO:0006793,GO:0006796,GO:0006807,GO:0006950,GO:0006979,GO:0007114,GO:0008033,GO:0008150,GO:0008152,GO:0008265,GO:0008641,GO:0009058,GO:0009108,GO:0009116,GO:0009117,GO:0009119,GO:0009141,GO:0009144,GO:0009150,GO:0009199,GO:0009205,GO:0009259,GO:0009451,GO:0009987,GO:0010467,GO:0010570,GO:0016070,GO:0016740,GO:0016772,GO:0016779,GO:0016782,GO:0016783,GO:0016874,GO:0016877,GO:0018117,GO:0018130,GO:0018175,GO:0019008,GO:0019538,GO:0019637,GO:0019693,GO:0019720,GO:0019954,GO:0030447,GO:0032324,GO:0032446,GO:0032447,GO:0032505,GO:0032991,GO:0033554,GO:0034227,GO:0034470,GO:0034599,GO:0034641,GO:0034660,GO:0036211,GO:0036267,GO:0040007,GO:0040008,GO:0042221,GO:0042278,GO:0042292,GO:0043170,GO:0043412,GO:0043545,GO:0044182,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046039,GO:0046128,GO:0046483,GO:0050789,GO:0050794,GO:0050896,GO:0051128,GO:0051186,GO:0051188,GO:0051189,GO:0051301,GO:0051716,GO:0055086,GO:0065007,GO:0070566,GO:0070647,GO:0070733,GO:0070783,GO:0070784,GO:0070887,GO:0071566,GO:0071704,GO:0072521,GO:0090304,GO:0090407,GO:1900428,GO:1901068,GO:1901135,GO:1901360,GO:1901362,GO:1901564,GO:1901566,GO:1901576,GO:1901657,GO:2000220
EggNOG free text desc.thiosulfate sulfurtransferase activity
EggNOG OGsCOG0476@1,KOG2017@2759
EC2.7.7.80,2.8.1.11
COG Functional cat.H
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03016,ko04121,ko04131
Exons2
Model size309
Cds size309
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930004.711941-CDS-H-paniculata_contig3252:1352..14391622930004.711941-CDS-H-paniculata_contig3252:1352..1439Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig3252 1353..1439 -
1691481106.8518617-CDS-H-paniculata_contig3252:1352..14391691481106.8518617-CDS-H-paniculata_contig3252:1352..1439Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig3252 1353..1439 -
1622930004.7241554-CDS-H-paniculata_contig3252:2343..25651622930004.7241554-CDS-H-paniculata_contig3252:2343..2565Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig3252 2344..2565 -
1691481106.8648756-CDS-H-paniculata_contig3252:2343..25651691481106.8648756-CDS-H-paniculata_contig3252:2343..2565Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig3252 2344..2565 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig3252.8746.1prot_H-paniculata_contig3252.8746.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig3252 1353..2565 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig3252.8746.1

>prot_H-paniculata_contig3252.8746.1 ID=prot_H-paniculata_contig3252.8746.1|Name=mRNA_H-paniculata_contig3252.8746.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=103bp
LPELNHDEVRRYSRHLILPDVGVRGQRTLKASSVLAVGTGGLGSPALLYL
AAAGVGRLGVLDDDVVDESNLQRQVIHGTSTLGKPKVESARDRMLDVNPH
VEV
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mRNA from alignment at H-paniculata_contig3252:1353..2565-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig3252.8746.1 ID=mRNA_H-paniculata_contig3252.8746.1|Name=mRNA_H-paniculata_contig3252.8746.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=1213bp|location=Sequence derived from alignment at H-paniculata_contig3252:1353..2565- (Halopteris paniculata Hal_grac_a_UBK monoicous)
TTGCCCGAGCTAAACCACGATGAAGTGCGGCGATATTCACGGCACTTGAT
CCTCCCGGATGTCGGTGTCCGCGGGCAGCGCACGCTCAAGGCCAGCTCTG
TGCTCGCCGTGGGCACCGGCGGCTTAGGGTCGCCGGCCCTCTTGTACCTG
GCGGCGGCCGGTGTTGGCAGGCTCGGTGTGCTCGACGATGATGTCGTTGA
CGAATCCAATCTACAGCGACAGGTAGCGTGGACTGTTTGGCATATCTGGG
AGTGGGTGCGGGTGGGAGTTTGGCGAGTTTGGGAGTCGACAAAAGCAGGG
TAGTTTGCGACCGCATGAGCTAATCGTTCATCGTTTGTCGCTCTTTTTAT
GTGTACCTCGAGAGGCTAGATCATGCTGTACTTGGTAGATGTATATTGTT
TAGAAGACAATGATTTTTCTGGTTGTTGTGGAGGTGCAGGGTCGTTTGTA
TTTGCCAACCCTCACATTATTTAGCTCATGTGCAACAATAGATGACAACT
TAGCTGGTGGGGAAAAGTGTTTCTCGGTCTTTTCCACCTTGATTCTGGCG
AGCGAATCGCTTCTTTCCTCGCTCGTGGCTGCCGCCACCGGTGCAGAACC
TTCCAGCCACTGATGCTGGGCGGCGACCGGGGCCTGCAATAGGGTCGCGA
GCATCAGTATTTGCCCGTTGCCGTTGAGCATTGGCCCATCTCGAACAAAT
ATACACCTGCGCCCTTCGTAAGCAAAAGTTGCTCACCACTGTGACCCAAC
AGTGATATATAATATGGGTACACACACATTCCATAACCCCACCAGAAGGC
GAGTCATGTCACCTCTACGCTGCACAGCTTTATCCCTTCCTCACTAAATA
AAAATGTAGTACGTAAAACGAGTACATTTCCCCTTGGTGGTGGTGGTGGT
GGTGGTGGTGTGCAATGACCACGGCGCTTACCGAAACGCATACAAAGGAA
CAGTCCGAAAAGAGGCGACTTGCGGCACCTCTGCAGAAGCAGGTGGTGTT
ATCGTAGTATGCAGTGTTTACCTATTTTAAATTGACGATTTCTCCATTGT
TTTGGTGCCGCTATTATTCAATGAACTGCCGTGTTACCCCGAACACACAA
TTACCCCCCCCCCCCCTCCCCCAAAGGTTATTCACGGAACCTCTACACTA
GGCAAGCCGAAAGTGGAATCGGCCAGAGACCGCATGCTGGACGTTAATCC
GCACGTTGAGGTG
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Coding sequence (CDS) from alignment at H-paniculata_contig3252:1353..2565-

>mRNA_H-paniculata_contig3252.8746.1 ID=mRNA_H-paniculata_contig3252.8746.1|Name=mRNA_H-paniculata_contig3252.8746.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=618bp|location=Sequence derived from alignment at H-paniculata_contig3252:1353..2565- (Halopteris paniculata Hal_grac_a_UBK monoicous)
TTGCCCGAGCTAAACCACGATGAAGTGCGGCGATATTCACGGCACTTGAT
CCTCCCGGATGTCGGTGTCCGCGGGCAGCGCACGCTCAAGGCCAGCTCTG
TGCTCGCCGTGGGCACCGGCGGCTTAGGGTCGCCGGCCCTCTTGTACCTG
GCGGCGGCCGGTGTTGGCAGGCTCGGTGTGCTCGACGATGATGTCGTTGA
CGAATCCAATCTACAGCGACAGTTGCCCGAGCTAAACCACGATGAAGTGC
GGCGATATTCACGGCACTTGATCCTCCCGGATGTCGGTGTCCGCGGGCAG
CGCACGCTCAAGGCCAGCTCTGTGCTCGCCGTGGGCACCGGCGGCTTAGG
GTCGCCGGCCCTCTTGTACCTGGCGGCGGCCGGTGTTGGCAGGCTCGGTG
TGCTCGACGATGATGTCGTTGACGAATCCAATCTACAGCGACAGGTTATT
CACGGAACCTCTACACTAGGCAAGCCGAAAGTGGAATCGGCCAGAGACCG
CATGCTGGACGTTAATCCGCACGTTGAGGTGGTTATTCACGGAACCTCTA
CACTAGGCAAGCCGAAAGTGGAATCGGCCAGAGACCGCATGCTGGACGTT
AATCCGCACGTTGAGGTG
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