mRNA_H-paniculata_contig27186.7436.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig27186.7436.1
Unique NamemRNA_H-paniculata_contig27186.7436.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: D8LNR1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNR1_ECTSI)

HSP 1 Score: 142 bits (359), Expect = 4.610e-39
Identity = 81/152 (53.29%), Postives = 104/152 (68.42%), Query Frame = 1 
Query:  112 DVSSHTLPASFVFLAVQLAFRKPLTVQKTSKNASSKRSQQRALENTERR--------LTKGGAFLPSDVALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDVSPFLAGLACDRFL 543
            + SS+TLPA+F F+AVQL F+   T Q T K    KR + +    T+R+         T  G +LP+DVA K  ILAA+KDLHGE+ S+ Y VDVLSWD  SG+G+LRV   SLVPIRAAL+ V S GD+ C++ V +VSPFLAGLAC+R+L
Sbjct:    2 EASSYTLPAAFCFIAVQLTFQ--ATRQATPKRP--KRGKVKGGGTTQRQQQAPQGSAATSQGVYLPTDVAFKTFILAAVKDLHGEVGSLAYPVDVLSWDAVSGEGRLRVPARSLVPIRAALSFVGSCGDVPCAIDVREVSPFLAGLACERYL 149
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: UPI001C64A4DA (ribonuclease P protein subunit p14 n=1 Tax=Centrocercus urophasianus TaxID=9002 RepID=UPI001C64A4DA)

HSP 1 Score: 58.2 bits (139), Expect = 4.260e-7
Identity = 38/92 (41.30%), Postives = 50/92 (54.35%), Query Frame = 1 
Query:  253 RRLTKGGAFLPSDVALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDVSPFLAGLA 528
            RR    GA  P    LK L++AAL++LHGE+      VDVL+++  S    LRV    LV + +ALTL   Y +  C+  V   SPFL  LA
Sbjct:   33 RRELPDGAARPGAPQLKQLVVAALRELHGEV-GAALPVDVLAYEEKSLCAVLRVISGGLVKLWSALTLQGFYQNRRCAFRVLQTSPFLLALA 123
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: UPI0003C10CDB (ribonuclease P protein subunit p14 n=1 Tax=Latimeria chalumnae TaxID=7897 RepID=UPI0003C10CDB)

HSP 1 Score: 56.6 bits (135), Expect = 1.590e-6
Identity = 32/76 (42.11%), Postives = 48/76 (63.16%), Query Frame = 1 
Query:  301 KGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDVSPFLAGLA 528
            K LI+AALK+LHGE  +     DVL+++  +    +RV  S LV + +A+TL+  Y +  C++ VT VSPFL  L+
Sbjct:   51 KQLIIAALKELHGETGAALPF-DVLTFEEKTTSAIVRVYSSGLVKLWSAITLLGYYQNQKCAIRVTQVSPFLLALS 125
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: A0A7K9YJJ3_9GALL (RPP14 protein (Fragment) n=7 Tax=Galliformes TaxID=8976 RepID=A0A7K9YJJ3_9GALL)

HSP 1 Score: 56.6 bits (135), Expect = 1.650e-6
Identity = 39/89 (43.82%), Postives = 50/89 (56.18%), Query Frame = 1 
Query:  271 GAFLPSDVALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDV---SPFLAGLA 528
            GA  P    LK L+LAAL++LHGE+      VDVL+++  S    LRV  S LV + +ALTL   Y +  C+  V  V   SPFL  LA
Sbjct:   40 GAARPGAPQLKQLVLAALRELHGEV-GAALPVDVLAYEEKSLCAILRVVSSGLVKLWSALTLQGFYQNRRCAFRVLQVGGTSPFLLALA 127
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: A0A4W3J7P9_CALMI (Uncharacterized protein n=5 Tax=Callorhinchus milii TaxID=7868 RepID=A0A4W3J7P9_CALMI)

HSP 1 Score: 55.8 bits (133), Expect = 2.820e-6
Identity = 35/81 (43.21%), Postives = 46/81 (56.79%), Query Frame = 1 
Query:  292 VALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDVSPFLAGLACD 534
            V  K LI+AAL+DLHGE+      VDVL+++       LRV    LV + +ALTL+  Y    C+  V  VSPFL  L+ D
Sbjct:   40 VQFKHLIVAALRDLHGEI-GAALPVDVLTFEEEILSAILRVQNRGLVKLWSALTLLGCYQGQKCAFRVLQVSPFLLALSGD 119
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: UPI0004574E47 (ribonuclease P protein subunit p14 isoform X1 n=1 Tax=Callorhinchus milii TaxID=7868 RepID=UPI0004574E47)

HSP 1 Score: 55.8 bits (133), Expect = 4.010e-6
Identity = 35/81 (43.21%), Postives = 46/81 (56.79%), Query Frame = 1 
Query:  292 VALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDVSPFLAGLACD 534
            V  K LI+AAL+DLHGE+      VDVL+++       LRV    LV + +ALTL+  Y    C+  V  VSPFL  L+ D
Sbjct:   58 VQFKHLIVAALRDLHGEI-GAALPVDVLTFEEEILSAILRVQNRGLVKLWSALTLLGCYQGQKCAFRVLQVSPFLLALSGD 137
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: A0A226PF64_COLVI (Uncharacterized protein n=1 Tax=Colinus virginianus TaxID=9014 RepID=A0A226PF64_COLVI)

HSP 1 Score: 55.8 bits (133), Expect = 5.240e-6
Identity = 37/85 (43.53%), Postives = 47/85 (55.29%), Query Frame = 1 
Query:  271 GAFLPSDVALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDVSPFLAGL 525
            GA  P    LK L+LAAL++LHGE+      VDVL++D  S    LRV  S LV + +ALTL  SY +  C+  V     F A L
Sbjct:   34 GAARPGAPQLKQLVLAALRELHGEV-GAALPVDVLAYDEKSLSAILRVVSSGLVKLWSALTLQGSYQNRRCAFRVLQEIRFPAPL 117
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: A0A226NF70_CALSU (Uncharacterized protein n=1 Tax=Callipepla squamata TaxID=9009 RepID=A0A226NF70_CALSU)

HSP 1 Score: 54.3 bits (129), Expect = 2.450e-5
Identity = 35/78 (44.87%), Postives = 44/78 (56.41%), Query Frame = 1 
Query:  271 GAFLPSDVALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDV 504
            GA  P    LK L+LAAL++LHGE+      VDVL +D  S    LRV  S LV + +ALTL  SY +  C+  V  V
Sbjct:   96 GAARPGAPQLKQLVLAALRELHGEV-GAALPVDVLVYDEKSLSAILRVVSSGLVKLWSALTLQGSYQNRRCAFRVLQV 172
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: A0A7L0PTX3_9AVES (RPP14 protein (Fragment) n=2 Tax=Neognathae TaxID=8825 RepID=A0A7L0PTX3_9AVES)

HSP 1 Score: 52.0 bits (123), Expect = 6.770e-5
Identity = 34/84 (40.48%), Postives = 48/84 (57.14%), Query Frame = 1 
Query:  259 LTKGGAFLPSDVALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDVSP 510
            L  GGA  PS    K L++AAL++LHGE+      VDVL+++  +    LRV  S LV + +ALTL+  Y +  C+  V  V P
Sbjct:   33 LPDGGAR-PSVAQFKQLVVAALRELHGEV-GAALPVDVLTYEEKTLSAILRVISSGLVKLWSALTLLGFYQNRRCAFRVLQVKP 114
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BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Match: UPI00093A4F6B (LOW QUALITY PROTEIN: ribonuclease P protein subunit p14 n=1 Tax=Meleagris gallopavo TaxID=9103 RepID=UPI00093A4F6B)

HSP 1 Score: 52.0 bits (123), Expect = 7.040e-5
Identity = 32/89 (35.96%), Postives = 48/89 (53.93%), Query Frame = 1 
Query:  271 GAFLPSDVALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQLRVAVSSLVPIRAALTLVSSYGDILCSVHVTDVSPFLAGLACDR 537
            GA  P    LK L++AAL++LHGE+      VDVL ++  S    LRV     V + +ALTL   Y +  C+  V   SPF + ++ ++
Sbjct:   10 GAARPGAPQLKQLVVAALRELHGEV-GAALPVDVLVYEEKSLCAVLRVVSGGFVKLWSALTLQGFYQNRRCAFRVLQTSPFXSCVSWEQ 97
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig27186.7436.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 11
Match NameE-valueIdentityDescription
D8LNR1_ECTSI4.610e-3953.29Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
UPI001C64A4DA4.260e-741.30ribonuclease P protein subunit p14 n=1 Tax=Centroc... [more]
UPI0003C10CDB1.590e-642.11ribonuclease P protein subunit p14 n=1 Tax=Latimer... [more]
A0A7K9YJJ3_9GALL1.650e-643.82RPP14 protein (Fragment) n=7 Tax=Galliformes TaxID... [more]
A0A4W3J7P9_CALMI2.820e-643.21Uncharacterized protein n=5 Tax=Callorhinchus mili... [more]
UPI0004574E474.010e-643.21ribonuclease P protein subunit p14 isoform X1 n=1 ... [more]
A0A226PF64_COLVI5.240e-643.53Uncharacterized protein n=1 Tax=Colinus virginianu... [more]
A0A226NF70_CALSU2.450e-544.87Uncharacterized protein n=1 Tax=Callipepla squamat... [more]
A0A7L0PTX3_9AVES6.770e-540.48RPP14 protein (Fragment) n=2 Tax=Neognathae TaxID=... [more]
UPI00093A4F6B7.040e-535.96LOW QUALITY PROTEIN: ribonuclease P protein subuni... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig27186contigH-paniculata_contig27186:1388..2914 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score64.7
Seed ortholog evalue4.1e-08
Seed eggNOG ortholog7955.ENSDARP00000016855
Preferred nameRPP14
KEGG koko:K14529
KEGG Pathwayko03013,map03013
Hectar predicted targeting categoryother localisation
GOsGO:0000966,GO:0001682,GO:0003674,GO:0003676,GO:0003723,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0006139,GO:0006396,GO:0006399,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0009987,GO:0010467,GO:0016070,GO:0030677,GO:0030681,GO:0031974,GO:0031981,GO:0032991,GO:0034470,GO:0034471,GO:0034641,GO:0034660,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0046483,GO:0070013,GO:0071704,GO:0090304,GO:0097159,GO:0099116,GO:1901360,GO:1901363,GO:1902494,GO:1902555,GO:1905348,GO:1990904
EggNOG free text desc.ribonuclease P
EggNOG OGs2BCSB@1,2S10S@2759,3A3XP@33154,3BRFU@33208,3D7R7@33213,48EKZ@7711,49BQG@7742,4A44A@7898
Ec32 ortholog descriptionRibonuclease P/MRP protein subunit
Ec32 orthologEc-08_006170.1
EC3.1.26.5
COG Functional cat.S
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03016
Exons3
Model size824
Cds size441
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig27186.7436.1prot_H-paniculata_contig27186.7436.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig27186 1666..2809 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929818.1647384-UTR-H-paniculata_contig27186:1387..16651622929818.1647384-UTR-H-paniculata_contig27186:1387..1665Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig27186 1388..1665 -
1691481024.9832568-UTR-H-paniculata_contig27186:1387..16651691481024.9832568-UTR-H-paniculata_contig27186:1387..1665Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig27186 1388..1665 -
1622929818.2237318-UTR-H-paniculata_contig27186:2809..29141622929818.2237318-UTR-H-paniculata_contig27186:2809..2914Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig27186 2810..2914 -
1691481025.0386834-UTR-H-paniculata_contig27186:2809..29141691481025.0386834-UTR-H-paniculata_contig27186:2809..2914Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig27186 2810..2914 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929818.1894834-CDS-H-paniculata_contig27186:1665..17891622929818.1894834-CDS-H-paniculata_contig27186:1665..1789Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig27186 1666..1789 -
1691481025.0074923-CDS-H-paniculata_contig27186:1665..17891691481025.0074923-CDS-H-paniculata_contig27186:1665..1789Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig27186 1666..1789 -
1622929818.2011337-CDS-H-paniculata_contig27186:2155..24131622929818.2011337-CDS-H-paniculata_contig27186:2155..2413Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig27186 2156..2413 -
1691481025.0196455-CDS-H-paniculata_contig27186:2155..24131691481025.0196455-CDS-H-paniculata_contig27186:2155..2413Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig27186 2156..2413 -
1622929818.2129006-CDS-H-paniculata_contig27186:2750..28091622929818.2129006-CDS-H-paniculata_contig27186:2750..2809Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig27186 2751..2809 -
1691481025.029617-CDS-H-paniculata_contig27186:2750..28091691481025.029617-CDS-H-paniculata_contig27186:2750..2809Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig27186 2751..2809 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig27186.7436.1

>prot_H-paniculata_contig27186.7436.1 ID=prot_H-paniculata_contig27186.7436.1|Name=mRNA_H-paniculata_contig27186.7436.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=147bp
MADVSSHTLPASFVFLAVQLAFRKPLTVQKTSKNASSKRSQQRALENTER
RLTKGGAFLPSDVALKGLILAALKDLHGEMESVTYLVDVLSWDPASGKGQ
LRVAVSSLVPIRAALTLVSSYGDILCSVHVTDVSPFLAGLACDRFL*
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mRNA from alignment at H-paniculata_contig27186:1388..2914-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig27186.7436.1 ID=mRNA_H-paniculata_contig27186.7436.1|Name=mRNA_H-paniculata_contig27186.7436.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=1527bp|location=Sequence derived from alignment at H-paniculata_contig27186:1388..2914- (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATCACAGCCCGTGCAGTAGTTGGGCTACCTTATTCTAGGCCTGCAGCACC
CGGTACTACACCACGCCTACACCAACGACGGCACTTGATTCTGAGTGAAG
ATATAATGGCAGACGTCTCATCCCACACGCTGCCAGCTAGTTTCGTTTTC
CTCGCCGTACAGTTGTGAGTGGCCTCAATTTCTCAATTTGTTTGACGTGA
GCTGTCGAAGGCTGCCTGAGCTCTCCTGCAGTGTACAACGCTTCGTCGAA
GTCTGTCAAGAACGTCAAATATTTGTCCAGTTCAATTGCTTGTTCGTTGT
CTCTTCTGACAAAGTAGTTACGAAATTTCTAGTCATCCATGCGGATGGAT
TGCCGATCGATAGGATGGCACGTGATGACAACACTTCGGGCATCGATGTT
ATATGGAGCACCTCTACTTACACTGAACTACAACTATACTCTATCATAAT
GTGATGTTGATGTTTTGCCCGTGGCTCTTACGCGTTCCTCTTGGAACCAA
GGGCATTTCGAAAGCCATTGACAGTCCAGAAGACATCCAAGAACGCATCG
TCCAAGCGAAGTCAACAGCGGGCGCTGGAAAATACAGAAAGACGACTTAC
AAAAGGAGGAGCATTTTTGCCTAGTGATGTAGCTCTTAAGGGGCTGATAC
TGGCTGCGTTGAAGGATCTTCATGGAGAGATGGAGTCCGTGACCTACCTC
GTCGACGTTCTGTCGTGGGATCCTGCCAGCGGTAAGGGACAACTACGGGT
GGCAGTCAGGTACGGCATCAGAATGTTCAGTTGTTTGTTCGCAAGATGTC
GAAGGGCTGCGATGGCTGTGGTAGATCGGTTGTTTGTGCATGCTGTGCTT
GAATCCCCGCCCCTGGTGTATGCCTTCCGAAAGCAAGAAATGTGTGTGAA
AGGGATGGCTTTACATACAAGCCTGCTGCATCTGGGGCACAGGCAGGCGG
GCGTGAGTGTCGCAGGTAGCTCAAACGAGTGTGCGCATTAGTTATTTAGT
GTGGACAGCAGACAGCGAAGTAATGGTGTTGCGGCTGCTGGCGTTGGTGT
TGTCCGAAATTACTTCCTCACAGATTCCTGCTTCGCTTCTTGCCGCTGCT
GCTGCTGCCTGCCGTGATTTTACAGCTCGCTGGTGCCAATTCGGGCGGCT
TTAACGTTGGTGTCGTCGTACGGTGATATTTTATGCTCCGTTCACGTCAC
CGATGTGTCTCCATTTTTGGCAGGCCTAGCGTGCGATCGATTTCTTTAGT
AGATGTGTTTTGCCCGAATTACAGAAAAAAAACTTTGGGCGTACAGTTTT
GTGTGTGTTTCGTTTGGTCCAGTGAGTGCTTCGCAAAAATGGCGTCGTTT
TGGCCACAGGTTAGTGTACGGCATGTTGAACACCTCAGCGAAATCCAGGA
AAATGTTTTGGCATGGGGAACTACTGTTCAGTTGACAGGCAGGGGCGACA
CCGCCAGAGCCCCCGGTCACCCAGGGCATTTAGCACAGATTAGTCAAGTA
CGAGAAGAAAAACTTGTTGGAATGGTA
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Coding sequence (CDS) from alignment at H-paniculata_contig27186:1388..2914-

>mRNA_H-paniculata_contig27186.7436.1 ID=mRNA_H-paniculata_contig27186.7436.1|Name=mRNA_H-paniculata_contig27186.7436.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=882bp|location=Sequence derived from alignment at H-paniculata_contig27186:1388..2914- (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGGCAGACGTCTCATCCCACACGCTGCCAGCTAGTTTCGTTTTCCTCGC
CGTACAGTTATGGCAGACGTCTCATCCCACACGCTGCCAGCTAGTTTCGT
TTTCCTCGCCGTACAGTTGGCATTTCGAAAGCCATTGACAGTCCAGAAGA
CATCCAAGAACGCATCGTCCAAGCGAAGTCAACAGCGGGCGCTGGAAAAT
ACAGAAAGACGACTTACAAAAGGAGGAGCATTTTTGCCTAGTGATGTAGC
TCTTAAGGGGCTGATACTGGCTGCGTTGAAGGATCTTCATGGAGAGATGG
AGTCCGTGACCTACCTCGTCGACGTTCTGTCGTGGGATCCTGCCAGCGGT
AAGGGACAACTACGGGTGGCAGTCAGGGCATTTCGAAAGCCATTGACAGT
CCAGAAGACATCCAAGAACGCATCGTCCAAGCGAAGTCAACAGCGGGCGC
TGGAAAATACAGAAAGACGACTTACAAAAGGAGGAGCATTTTTGCCTAGT
GATGTAGCTCTTAAGGGGCTGATACTGGCTGCGTTGAAGGATCTTCATGG
AGAGATGGAGTCCGTGACCTACCTCGTCGACGTTCTGTCGTGGGATCCTG
CCAGCGGTAAGGGACAACTACGGGTGGCAGTCAGCTCGCTGGTGCCAATT
CGGGCGGCTTTAACGTTGGTGTCGTCGTACGGTGATATTTTATGCTCCGT
TCACGTCACCGATGTGTCTCCATTTTTGGCAGGCCTAGCGTGCGATCGAT
TTCTTTAGCTCGCTGGTGCCAATTCGGGCGGCTTTAACGTTGGTGTCGTC
GTACGGTGATATTTTATGCTCCGTTCACGTCACCGATGTGTCTCCATTTT
TGGCAGGCCTAGCGTGCGATCGATTTCTTTAG
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