mRNA_H-paniculata_contig5118.12426.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig5118.12426.1
Unique NamemRNA_H-paniculata_contig5118.12426.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Match: D8LFE6_ECTSI (Peroxidase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LFE6_ECTSI)

HSP 1 Score: 139 bits (349), Expect = 2.220e-35
Identity = 65/135 (48.15%), Postives = 85/135 (62.96%), Query Frame = 1 
Query:    4 VWGEQLYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVFAPPEVSVCISDCADENSSEISLSDKYSMVVEQRSDDGSVAFTLRARSVGTQAMMGIGFGGTMMVEAQDFVICEILDESTAECIDRQ 408
             W +Q+YRT  GDR  H HS  IED+  TT+ D++ RT G  DLP S FA   V VC +DC      ++ LSD Y M  E + D+  ++F+L AR +G + M+G+GFGG  M +AQDFVICE+     AECIDRQ
Sbjct:  515 AWMDQMYRTFRGDRFHHLHSRSIEDVSLTTISDLLNRTTGAADLPLSAFAAAGVEVCGADCTAVGEEDVELSDSYKMAWEVQEDEELISFSLSARDIGDRGMLGVGFGGLTMSDAQDFVICELTSTDEAECIDRQ 649
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BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Match: A0A6H5JBZ5_9PHAE (DOMON domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBZ5_9PHAE)

HSP 1 Score: 127 bits (319), Expect = 1.530e-32
Identity = 62/138 (44.93%), Postives = 83/138 (60.14%), Query Frame = 1 
Query:    4 VWGEQLYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVFAPPEVSVCISDCADENSSEISLSDKYSMVVEQRSDDGSVAFTLRARSVGTQAMMGIGFGGTMMVEAQDFVICEILDESTAECIDRQSIT 417
             W +QL R+I GDR +H H   +E++ NTTL D++ R    TDLP SVF  P ++VC   CA +      LSD + +  E+  DD  +A T R + +GT   MG+G+GG  M  AQD++ICEI DESTA C DR   T
Sbjct:  123 AWSDQLTRSIAGDRFYHLHVRNMENVANTTLMDVIGRVTNATDLPLSVFQAPSITVCDGGCAGDGDGVAVLSDNFELEWEELEDD-QMAITFRCKDLGTSGWMGVGWGGLTMELAQDYIICEITDESTASCTDRAYTT 259
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BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Match: A0A6H5JBL8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBL8_9PHAE)

HSP 1 Score: 130 bits (326), Expect = 2.800e-32
Identity = 65/136 (47.79%), Postives = 85/136 (62.50%), Query Frame = 1 
Query:    4 VWGEQLYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVFAPPEVSVCISDCADENSSEISLSDKYSMVVEQRSDDGSVAFTLRARSVGTQAMMGIGFGGTMMVEAQDFVICEILDESTAECIDRQS 411
             W EQ+YR   GDR  H HS  IED   TT+ D++ RT G TD+P S F    V VC +DC+    SE  LSD YS+  E   +D ++  +L A+ +G   M+GIG+GG  M +AQDFVICE+L  + AECIDRQ+
Sbjct:  630 AWLEQMYRAFQGDRFHHLHSRDIEDASLTTISDLINRTTGATDIPLSAFVAATVEVCGADCSSIGVSEADLSDSYSIAWEVL-EDNTMTMSLSAKGIGDTGMLGIGWGGLTMSDAQDFVICEVLSSTEAECIDRQA 764
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BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Match: D7FQW8_ECTSI (Peroxidase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQW8_ECTSI)

HSP 1 Score: 129 bits (323), Expect = 6.960e-32
Identity = 63/138 (45.65%), Postives = 84/138 (60.87%), Query Frame = 1 
Query:    4 VWGEQLYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVFAPPEVSVCISDCADENSSEISLSDKYSMVVEQRSDDGSVAFTLRARSVGTQAMMGIGFGGTMMVEAQDFVICEILDESTAECIDRQSIT 417
             W +QL R+I GDR +H H+  +E++ NTTL D++ R    TDLP SVF  P ++VC   CA +      LSD + +  E+  DD  +A T R + +GT   MG+G+GG  M  AQDF+ICEI DESTA C DR   T
Sbjct:  390 AWSDQLTRSIAGDRFYHLHARYMENVANTTLMDVIGRVTNATDLPLSVFQAPSITVCDGGCAGDGDGIAVLSDNFELEWEELEDD-QMAITFRCKDLGTSGWMGVGWGGLTMELAQDFIICEITDESTASCTDRAYTT 526
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BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Match: D7G7J2_ECTSI (Peroxidase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7J2_ECTSI)

HSP 1 Score: 125 bits (315), Expect = 8.400e-31
Identity = 61/134 (45.52%), Postives = 85/134 (63.43%), Query Frame = 1 
Query:    4 VWGEQLYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVFAPPEVSVCISDCADENSSEISLSDKYSMVVEQRSDDGSVAFTLRARSVGTQAMMGIGFGGTMMVEAQDFVICEILDESTAECIDR 405
             W  QLYRT  GDR  H HS PIE++   ++  ++ +T+G+TDLP S F  PEV+VC  +C     S +SL+++Y+M  E   DD +++ +L    +G   MMGIGFGG  M +AQDF+ICE+     AECIDR
Sbjct:  423 AWVNQLYRTFFGDRYHHLHSRPIENVSLASISGLINQTLGVTDLPASGFTVPEVTVCTGECEAAGISGVSLAERYAMSWEVI-DDQTISISLSVLGIGDSGMMGIGFGGLSMTDAQDFIICEVFSTGGAECIDR 555
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BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Match: A0A6H5JQ31_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ31_9PHAE)

HSP 1 Score: 124 bits (311), Expect = 2.870e-30
Identity = 61/134 (45.52%), Postives = 85/134 (63.43%), Query Frame = 1 
Query:    4 VWGEQLYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVFAPPEVSVCISDCADENSSEISLSDKYSMVVEQRSDDGSVAFTLRARSVGTQAMMGIGFGGTMMVEAQDFVICEILDESTAECIDR 405
             W  QLYRT  GDR  H HS PIE++   ++  ++ +T+G+TDLP S F  PEV+VC  +C     S +SL+++Y+M  E   DD S++ +L    +G   M+G+GFGG  M +AQDFVICE+     AECIDR
Sbjct:  408 AWVYQLYRTFFGDRYHHLHSRPIENVSLASISGLINQTLGVTDLPESGFMVPEVTVCTGECEAAGISGVSLAERYAMSWEI--DDQSISISLSVLDIGDSGMIGVGFGGESMTDAQDFVICEVFSGGDAECIDR 539
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BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Match: D7G7J1_ECTSI (Peroxidase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7J1_ECTSI)

HSP 1 Score: 118 bits (296), Expect = 3.040e-28
Identity = 57/134 (42.54%), Postives = 83/134 (61.94%), Query Frame = 1 
Query:    4 VWGEQLYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVFAPPEVSVCISDCADENSSEISLSDKYSMVVEQRSDDGSVAFTLRARSVGTQAMMGIGFGGTMMVEAQDFVICEILDESTAECIDR 405
             W +QLYR++ GDR  H HS PIE++   ++  ++ RT+GLT LP S F  PEV+VC   C    +S +SL+++Y +  E   +D ++  +L    +G   M+GIGFGG  M +AQDF+ICE+     AEC DR
Sbjct:  423 AWVDQLYRSLFGDRYHHLHSRPIENVSLVSISQLLNRTLGLTALPESGFTVPEVTVCTGQCEATGTSGVSLAERYGISWEV--EDETLLISLSVLGIGDSGMIGIGFGGLSMTDAQDFIICEVFSTGGAECTDR 554
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BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Match: D7FX04_ECTSI (Dopamine beta-monooxygenase, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FX04_ECTSI)

HSP 1 Score: 117 bits (292), Expect = 1.040e-27
Identity = 59/131 (45.04%), Postives = 80/131 (61.07%), Query Frame = 1 
Query:   19 LYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVFAPPEVSVCISDCADENSSEISLSDKYSMVVEQRSDDGSVAFTLRARSVGTQAMMGIGFGGTMMVEAQDFVICEILDESTAECIDRQS 411
            + R   GDR  H HS  IED   TT+ D++ RT G  D+P S F    V VC +DC+    SE  LSD YS+  E   +D ++  +L A+ +G   M+GIG+GG  M +AQD+VICE+L  + AECIDRQ+
Sbjct:    1 MKRAFQGDRFHHLHSRDIEDASLTTISDLINRTTGAADIPLSAFVAAAVEVCGADCSSIGVSEADLSDSYSVAWEMV-EDNTMTMSLSAKGIGDTGMLGIGWGGLTMSDAQDYVICEVLSSTEAECIDRQA 130
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BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Match: D8LB73_ECTSI (Peroxinectin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LB73_ECTSI)

HSP 1 Score: 94.4 bits (233), Expect = 8.770e-20
Identity = 52/139 (37.41%), Postives = 81/139 (58.27%), Query Frame = 1 
Query:    4 VWGEQLYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVFAPPEVSVCISDCADENSSEISLSDKYSMVVEQRS---DDGSVAFTLRARSVGTQAMMGIGFGGTMMVEAQDFVICEILDESTAECIDRQS 411
            V+ EQLYR I+GDR  H HS   ED   +TL ++++    ++ +P   F+ P++ V  S+C+    +E++L++ Y +   Q     + G +  TL AR +  Q M+ IGFGG  M  A DFVIC +  E++AEC DR +
Sbjct:  492 VFLEQLYRAIVGDRHHHSHSTQNEDAALSTLKNLILNNSLVSSIPLDSFSAPDL-VTSSNCSSTEMNEVTLAEGYKLAWNQSDLVEEGGDMFITLSARGIEGQGMIAIGFGGLSMETADDFVICVVESETSAECTDRSA 629
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig5118.12426.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 9
Match NameE-valueIdentityDescription
D8LFE6_ECTSI2.220e-3548.15Peroxidase n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A6H5JBZ5_9PHAE1.530e-3244.93DOMON domain-containing protein n=1 Tax=Ectocarpus... [more]
A0A6H5JBL8_9PHAE2.800e-3247.79Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FQW8_ECTSI6.960e-3245.65Peroxidase n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
D7G7J2_ECTSI8.400e-3145.52Peroxidase n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A6H5JQ31_9PHAE2.870e-3045.52Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G7J1_ECTSI3.040e-2842.54Peroxidase n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
D7FX04_ECTSI1.040e-2745.04Dopamine beta-monooxygenase, putative n=1 Tax=Ecto... [more]
D8LB73_ECTSI8.770e-2037.41Peroxinectin n=1 Tax=Ectocarpus siliculosus TaxID=... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig5118contigH-paniculata_contig5118:11041..13168 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score141.7
Seed ortholog evalue2.5e-31
Seed eggNOG ortholog2880.D8LFE6
Hectar predicted targeting categoryother localisation
EggNOG free text desc.peroxidase activity
EggNOG OGsKOG2408@1,KOG2408@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons3
Model size420
Cds size417
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig5118.12426.1prot_H-paniculata_contig5118.12426.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig5118 11041..13166 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930500.6818154-CDS-H-paniculata_contig5118:11040..112641622930500.6818154-CDS-H-paniculata_contig5118:11040..11264Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig5118 11041..11264 +
1691481320.695993-CDS-H-paniculata_contig5118:11040..112641691481320.695993-CDS-H-paniculata_contig5118:11040..11264Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig5118 11041..11264 +
1622930500.6971004-CDS-H-paniculata_contig5118:12682..127701622930500.6971004-CDS-H-paniculata_contig5118:12682..12770Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig5118 12683..12770 +
1691481320.7119136-CDS-H-paniculata_contig5118:12682..127701691481320.7119136-CDS-H-paniculata_contig5118:12682..12770Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig5118 12683..12770 +
1622930500.7151382-CDS-H-paniculata_contig5118:13061..131661622930500.7151382-CDS-H-paniculata_contig5118:13061..13166Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig5118 13062..13166 +
1691481320.7225509-CDS-H-paniculata_contig5118:13061..131661691481320.7225509-CDS-H-paniculata_contig5118:13061..13166Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig5118 13062..13166 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig5118.12426.1

>prot_H-paniculata_contig5118.12426.1 ID=prot_H-paniculata_contig5118.12426.1|Name=mRNA_H-paniculata_contig5118.12426.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=139bp
EVWGEQLYRTILGDRLWHRHSNPIEDIENTTLYDIMVRTVGLTDLPFSVF
APPEVSVCISDCADENSSEISLSDKYSMVVEQRSDDGSVAFTLRARSVGT
QAMMGIGFGGTMMVEAQDFVICEILDESTAECIDRQSIT
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mRNA from alignment at H-paniculata_contig5118:11041..13168+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig5118.12426.1 ID=mRNA_H-paniculata_contig5118.12426.1|Name=mRNA_H-paniculata_contig5118.12426.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=2128bp|location=Sequence derived from alignment at H-paniculata_contig5118:11041..13168+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
GAGGTGTGGGGAGAGCAGCTGTACCGGACCATACTTGGAGACCGGCTGTG
GCATCGGCACTCGAACCCCATCGAGGACATCGAAAACACCACCCTGTACG
ACATCATGGTGCGGACCGTGGGTCTAACAGACCTGCCGTTCTCCGTGTTC
GCCCCCCCCGAGGTGTCCGTATGCATTTCCGATTGCGCAGATGAGAATTC
TTCGGAAATTTCTCTCTCTGACAAGTGAGTGACAGCTCCCCCCCCATCTG
TCTTTGATTTGACTTCGCTGATTGGTGTATACAACTGAAAGTTCAGTTGA
CCTGCGTAAACCTCAACAGGGGTACGATGACGTGTTAACATAATCGGTAT
AGTTGTACTAATTGGGGATTCCTTCCCACTCCCCCTTGCCAGAAAATGGA
ACCGTTGATTGTACTGAAGTTAAAATGGAGAACATGTAATACGCGGCCGA
GTGATCTATTGTACAACGGGTGGCGGACTCGCCAGGAGTAACTTAGACGT
GTCGTGATGTACGCCGCATTCAGTGAGGTATTTTCAAAACTAAAAATCAT
ATCCCCCAAATCTCAAGTTACGACTCGATCTTTTAAAATAAATAATGGTT
TTTGGACCCCATTTTATACTACACCATTGTTTTCAAGTGTCCCTTTGTTG
TTCTGATCGTGTATTTATAGCAGTATAGCCACTACCTAACCTACCCTTCC
CATGCAGTTCGTGTCTCGTCCCCCGTCTCCATTTATGGAAATCCCATGAC
CCACACACAACGGCTCGATGCGCCTCGCAAATAAGCACATTGTTTGGGAA
CGGTCTTTATATTCTTGAGTGGACCAGAGTTGAAAAAATTGTTTGTTCGG
GAAGGAGCATCGTCGGTCACCTTGCCGTACGATGTAATTAACTGAGAGTA
TGAGCAAAGTACGTGTGCAAAACGTACCGCTTTATAAATATTATCACCCC
GTCTGTGCGTTACGCTTCCCGGTAACCCAAACACGTATGTGTGAGTTGGA
AAAACGAAGCCAACGAGGGGCGTTATTAAACGTGGGCAAGCTTTGGCCCT
CCCTCTCCCTTTTCCCGTGTAGTTTTCAGTGTGGGGGCGGTAGCAGGCTC
TGATATTTTCTACAATTTCCAAGTCGATGTGGAGCAGCCGATTTAGAAAT
TCAGGATCAAGCACGCGTCTTGAGGATGAAATATATTTTTCCAGCTAGCT
ACTGCAGCTTGGGACAATATCTTTACTAGCAATAGCCAAACCCTGGATGG
AAGAATGAATTCAAACTTAAAAACAACTGTCTTCTCACTAGCTGTCTAAC
GAATGCTCCTATTTTCGAGGGAATTTGATTCATCAGTTAAAGTCGAGAGA
GTGGAAATATGTGTCCATCATTAGAAACAATATATATCCCAAACTCGGAA
AAGTGAGTTTGCCACTCGGTCCCGCTACATGACTCTCTGCCGTACTTGAC
ATATGTTTCTCCTGACATTTGGTTAGTGGAGTTGCCACTATCGCTGCGAA
TCTTAGGCTCAGACCAAGTCGATCCAGGCGCTGGTTTCAAGTGCTCGTAT
CCCGGTCAACCGTTCGATATGCGCAGCAACAAGCAAGACATGTATCTACA
GTGTACGTGGTCGAATATATGTATATGTATTGAACGTATCAGGTATTCCA
TGGTGGTTGAACAGAGGAGCGACGATGGGAGCGTGGCGTTTACCCTCAGA
GCAAGGTCTGTCGGCACCCAGGCCATGATGGTGAGTGGTTTGCGTTTTTT
GTCACACTGGTCGGTGCGGCCAGTTCGACACGAATTTTCCCAGGAAAACT
CTAGTGTGCAAACTGGCAACCTTGGGGTACACATACCAGTGCTTGTCCAT
TGATTGGTCTGTGCTGCTTGTTTTGTTTTCAGCGTTTCGTTCTGTCTGCT
CGGGTTGGCCCTTTTGGTTCCCCCTCCCTTCTACGGTCTTGTCTTTTTTA
TCTGTCATTCATCGTGCACTTTGTGTATCTGCGGTTTTGTATCTCTTTTC
GCTTCAACAAACAAAAAACAGGGGATTGGCTTCGGTGGCACCATGATGGT
AGAGGCCCAAGACTTTGTGATCTGCGAGATTCTCGACGAATCCACCGCTG
AGTGCATCGATCGACAGTCCATTACCAG
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Coding sequence (CDS) from alignment at H-paniculata_contig5118:11041..13168+

>mRNA_H-paniculata_contig5118.12426.1 ID=mRNA_H-paniculata_contig5118.12426.1|Name=mRNA_H-paniculata_contig5118.12426.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=834bp|location=Sequence derived from alignment at H-paniculata_contig5118:11041..13168+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
GAGGTGTGGGGAGAGCAGCTGTACCGGACCATACTTGGAGACCGGCTGTG
GCATCGGCACTCGAACCCCATCGAGGACATCGAAAACACCACCCTGTACG
ACATCATGGTGCGGACCGTGGGTCTAACAGACCTGCCGTTCTCCGTGTTC
GCCCCCCCCGAGGTGTCCGTATGCATTTCCGATTGCGCAGATGAGAATTC
TTCGGAAATTTCTCTCTCTGACAAGAGGTGTGGGGAGAGCAGCTGTACCG
GACCATACTTGGAGACCGGCTGTGGCATCGGCACTCGAACCCCATCGAGG
ACATCGAAAACACCACCCTGTACGACATCATGGTGCGGACCGTGGGTCTA
ACAGACCTGCCGTTCTCCGTGTTCGCCCCCCCCGAGGTGTCCGTATGCAT
TTCCGATTGCGCAGATGAGAATTCTTCGGAAATTTCTCTCTCTGACAAGT
ATTCCATGGTGGTTGAACAGAGGAGCGACGATGGGAGCGTGGCGTTTACC
CTCAGAGCAAGGTCTGTCGGCACCCAGGCCATGATGGTATTCCATGGTGG
TTGAACAGAGGAGCGACGATGGGAGCGTGGCGTTTACCCTCAGAGCAAGG
TCTGTCGGCACCCAGGCCATGATGGGGATTGGCTTCGGTGGCACCATGAT
GGTAGAGGCCCAAGACTTTGTGATCTGCGAGATTCTCGACGAATCCACCG
CTGAGTGCATCGATCGACAGTCCATTACCGGGATTGGCTTCGGTGGCACC
ATGATGGTAGAGGCCCAAGACTTTGTGATCTGCGAGATTCTCGACGAATC
CACCGCTGAGTGCATCGATCGACAGTCCATTACC
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