mRNA_H-paniculata_contig32122.8649.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig32122.8649.1
Unique NamemRNA_H-paniculata_contig32122.8649.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
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BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: A0A6H5JE49_9PHAE (RING-type domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JE49_9PHAE)

HSP 1 Score: 146 bits (369), Expect = 1.440e-36
Identity = 67/156 (42.95%), Postives = 97/156 (62.18%), Query Frame = 1 
Query:  346 MNKAKGRIAMQCPICNERRT--KGVECSEGHFVCKTCCVQYVSLTL--EGTIFWDTIECVNYPCKSYYRGVEIREVMPQSAIQRIEKRQMDAAYLVAGERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTMCRKDFWWTCNCEYSGQHKTGC 801
            + +  GR A+ C +C E     +   C EGH +CK C   YV  TL  +GT+FWD I+C       +  G  +  ++P   I++IE +QMD A+LVAGERDP+S   I KHS+ CP CG+PIQ++ GC H+ C +CR +FW+ C+C+Y  +H+ GC
Sbjct:  101 IRRKAGRAAVTCIVCFEDTAVDRCSRCPEGHAMCKNCVTSYVVETLVPQGTVFWDRIKCGGSSTCGHLFGPSVTSLLPIPIIKKIETKQMDVAHLVAGERDPTSDKWISKHSRDCPNCGVPIQRSSGCMHMTCIICRHNFWYDCDCKYP-RHRQGC 255
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BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: A0A074XDQ5_9PEZI (RING-type domain-containing protein (Fragment) n=1 Tax=Aureobasidium namibiae CBS 147.97 TaxID=1043004 RepID=A0A074XDQ5_9PEZI)

HSP 1 Score: 76.3 bits (186), Expect = 4.230e-12
Identity = 32/64 (50.00%), Postives = 41/64 (64.06%), Query Frame = 1 
Query:  607 AAYLVAGERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTMCRKDFWWTCNCEYSGQHKTG 798
            AA   + E D  SQ  IDK SKPCP+C +PIQKN GCDH+ C+ C  DF W C+ +Y+   + G
Sbjct:  141 AALHRSTEHDEKSQKEIDKISKPCPQCRVPIQKNSGCDHMTCSRCNHDFCWACSADYNDIARQG 204
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BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: A0A074VYK1_9PEZI (RING-type domain-containing protein (Fragment) n=1 Tax=Aureobasidium melanogenum CBS 110374 TaxID=1043003 RepID=A0A074VYK1_9PEZI)

HSP 1 Score: 70.5 bits (171), Expect = 4.020e-10
Identity = 27/57 (47.37%), Postives = 36/57 (63.16%), Query Frame = 1 
Query:  628 ERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTMCRKDFWWTCNCEYSGQHKTG 798
            E D  S+  ++K SKPCP C +PIQKN GCDH+ C+ CR  F W C   Y+  ++ G
Sbjct:  148 ENDEKSEKEVEKISKPCPECRVPIQKNNGCDHMTCSRCRHQFCWICLASYTHIYRDG 204
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BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: S7PIK8_MYOBR (RBR-type E3 ubiquitin transferase n=1 Tax=Myotis brandtii TaxID=109478 RepID=S7PIK8_MYOBR)

HSP 1 Score: 68.6 bits (166), Expect = 5.180e-10
Identity = 31/75 (41.33%), Postives = 48/75 (64.00%), Query Frame = 1 
Query:  568 QSAIQRI--EKRQMDAAYLVAGERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTM--CRKDFWWTCNCEYS 780
            Q+A+ R+  +  Q+DA        + SS+  I K +KPCPRC +P++KNGGC H+KC    C+ ++ W C+CE+S
Sbjct:   70 QTALTRLLPQAYQVDARAAEQARWEESSRETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCQLEWCWNCSCEWS 144
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BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: UPI000B4F1927 (E3 ubiquitin-protein ligase parkin n=1 Tax=Meriones unguiculatus TaxID=10047 RepID=UPI000B4F1927)

HSP 1 Score: 66.6 bits (161), Expect = 7.270e-10
Identity = 32/92 (34.78%), Postives = 50/92 (54.35%), Query Frame = 1 
Query:  523 CKSYYRGVEIREVMPQSAIQ----RIEKRQMDAAYLVAGERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTM--CRKDFWWTCNCEYS 780
            CK  Y   +   +   S       R++KR  + A       + +S+  I K +KPCPRC +P++KNGGC H+KC    CR ++ W C+CE++
Sbjct:    9 CKEAYHEGDCNSLFEASGATSQAYRVDKRAAEQARW-----EEASKETIKKTTKPCPRCNVPVEKNGGCMHMKCPQPQCRLEWCWNCSCEWN 95
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BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: A0A8C6H7B7_MUSSI (RBR-type E3 ubiquitin transferase n=1 Tax=Mus spicilegus TaxID=10103 RepID=A0A8C6H7B7_MUSSI)

HSP 1 Score: 65.1 bits (157), Expect = 1.490e-9
Identity = 28/68 (41.18%), Postives = 43/68 (63.24%), Query Frame = 1 
Query:  583 RIEKRQMDAAYLVAGERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTM--CRKDFWWTCNCEYS 780
            R++KR  + A       + +S+  I K +KPCPRC +PI+KNGGC H+KC    C+ ++ W C CE++
Sbjct:   13 RVDKRAAERARW-----EEASKETIKKTTKPCPRCNVPIEKNGGCMHMKCPQPQCKLEWCWNCGCEWN 75
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BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: A0A6A6PSR3_9PEZI (RING-type domain-containing protein n=1 Tax=Neohortaea acidophila TaxID=245834 RepID=A0A6A6PSR3_9PEZI)

HSP 1 Score: 69.7 bits (169), Expect = 1.530e-9
Identity = 29/69 (42.03%), Postives = 40/69 (57.97%), Query Frame = 1 
Query:  628 ERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTMCRKDFWWTCNCEYSG-------QHKTGCAAWR 813
            + +  S   + K++K CP CG   QKNGGCDH++CTMCR +F + C  +Y          HK+ CA WR
Sbjct:  152 KEEDKSTAALKKYAKLCPGCGRKFQKNGGCDHIRCTMCRHEFCYLCFADYKDIFGKGNHAHKSSCAHWR 220
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BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: A0A1V8SMJ7_9PEZI (RING-type domain-containing protein n=2 Tax=Rachicladosporium TaxID=470018 RepID=A0A1V8SMJ7_9PEZI)

HSP 1 Score: 69.3 bits (168), Expect = 1.540e-9
Identity = 39/105 (37.14%), Postives = 50/105 (47.62%), Query Frame = 1 
Query:  469 LTLEGTIFWDTIECVNYPCKSYYRGVEIREVMPQSAIQRIEKRQMDAAYLVAGERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTMCRKDFWWTCNCEYSG 783
            LT E   F     C++  C S   G    E       Q   +  +D A L     +  S  L+ K SKPCP+CGI +QK GGCDH+ C  CR +F W C+  Y G
Sbjct:  120 LTTEEEFF----SCISAACSS---GAPFHEEKTCEEHQNALRVAVDHAEL-----NRESAALVKKISKPCPKCGISVQKAGGCDHMTCMKCRHEFCWPCSAAYRG 212
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BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: A0A8C0L2Y8_CANLU (RBR-type E3 ubiquitin transferase n=1 Tax=Canis lupus dingo TaxID=286419 RepID=A0A8C0L2Y8_CANLU)

HSP 1 Score: 65.1 bits (157), Expect = 1.700e-9
Identity = 24/51 (47.06%), Postives = 38/51 (74.51%), Query Frame = 1 
Query:  634 DPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTM--CRKDFWWTCNCEYS 780
            + +S+  I K +KPCPRC +P++KNGGC H+KC    C+ ++ WTC+CE++
Sbjct:   30 EEASKETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCQLEWCWTCSCEWN 80
[Close]
BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Match: A0A5F9DAU5_RABIT (RBR-type E3 ubiquitin transferase n=1 Tax=Oryctolagus cuniculus TaxID=9986 RepID=A0A5F9DAU5_RABIT)

HSP 1 Score: 64.7 bits (156), Expect = 2.080e-9
Identity = 30/68 (44.12%), Postives = 45/68 (66.18%), Query Frame = 1 
Query:  583 RIEKRQMDAAYLVAGERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKC--TMCRKDFWWTCNCEYS 780
            R+++R   AA L   E   +S+  I K +KPCPRC +P++KNGGC H+KC  + CR ++ W C CE++
Sbjct:   14 RVDER---AAELARWEE--ASKDTIQKTTKPCPRCHVPVEKNGGCMHMKCPQSQCRLEWCWHCGCEWN 76
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig32122.8649.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JE49_9PHAE1.440e-3642.95RING-type domain-containing protein n=2 Tax=Ectoca... [more]
A0A074XDQ5_9PEZI4.230e-1250.00RING-type domain-containing protein (Fragment) n=1... [more]
A0A074VYK1_9PEZI4.020e-1047.37RING-type domain-containing protein (Fragment) n=1... [more]
S7PIK8_MYOBR5.180e-1041.33RBR-type E3 ubiquitin transferase n=1 Tax=Myotis b... [more]
UPI000B4F19277.270e-1034.78E3 ubiquitin-protein ligase parkin n=1 Tax=Merione... [more]
A0A8C6H7B7_MUSSI1.490e-941.18RBR-type E3 ubiquitin transferase n=1 Tax=Mus spic... [more]
A0A6A6PSR3_9PEZI1.530e-942.03RING-type domain-containing protein n=1 Tax=Neohor... [more]
A0A1V8SMJ7_9PEZI1.540e-937.14RING-type domain-containing protein n=2 Tax=Rachic... [more]
A0A8C0L2Y8_CANLU1.700e-947.06RBR-type E3 ubiquitin transferase n=1 Tax=Canis lu... [more]
A0A5F9DAU5_RABIT2.080e-944.12RBR-type E3 ubiquitin transferase n=1 Tax=Oryctola... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig32122contigH-paniculata_contig32122:102..1807 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score95.1
Seed ortholog evalue5.2e-17
Seed eggNOG ortholog2880.D8LB34
KEGG koko:K11967,ko:K11973,ko:K11977
Hectar predicted targeting categoryother localisation
EggNOG free text desc.ubiquitin conjugating enzyme binding
EggNOG OGsKOG1815@1,KOG1815@2759
EC2.3.2.31
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko04121
Exons1
Model size1706
Cds size810
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig32122.8649.1prot_H-paniculata_contig32122.8649.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig32122 111..920 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929991.089997-UTR-H-paniculata_contig32122:101..1101622929991.089997-UTR-H-paniculata_contig32122:101..110Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig32122 102..110 +
1691481100.9662993-UTR-H-paniculata_contig32122:101..1101691481100.9662993-UTR-H-paniculata_contig32122:101..110Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig32122 102..110 +
1622929991.1210504-UTR-H-paniculata_contig32122:920..18071622929991.1210504-UTR-H-paniculata_contig32122:920..1807Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig32122 921..1807 +
1691481101.0048208-UTR-H-paniculata_contig32122:920..18071691481101.0048208-UTR-H-paniculata_contig32122:920..1807Halopteris paniculata Hal_grac_a_UBK monoicousUTRH-paniculata_contig32122 921..1807 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929991.1064792-CDS-H-paniculata_contig32122:110..9201622929991.1064792-CDS-H-paniculata_contig32122:110..920Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig32122 111..920 +
1691481100.98984-CDS-H-paniculata_contig32122:110..9201691481100.98984-CDS-H-paniculata_contig32122:110..920Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig32122 111..920 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig32122.8649.1

>prot_H-paniculata_contig32122.8649.1 ID=prot_H-paniculata_contig32122.8649.1|Name=mRNA_H-paniculata_contig32122.8649.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=270bp
MAHTMEPARTASKDEATWTPSTRDRGIVDSMVHNRSTETPDVVDVTGSET
LSETATAEQERKRKRYYGVVDLTESGVFSEMAPFESKGKGKGKRDVVDVE
AAWEQYAQLSAIMNKAKGRIAMQCPICNERRTKGVECSEGHFVCKTCCVQ
YVSLTLEGTIFWDTIECVNYPCKSYYRGVEIREVMPQSAIQRIEKRQMDA
AYLVAGERDPSSQTLIDKHSKPCPRCGIPIQKNGGCDHVKCTMCRKDFWW
TCNCEYSGQHKTGCAAWRR*
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mRNA from alignment at H-paniculata_contig32122:102..1807+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig32122.8649.1 ID=mRNA_H-paniculata_contig32122.8649.1|Name=mRNA_H-paniculata_contig32122.8649.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=1706bp|location=Sequence derived from alignment at H-paniculata_contig32122:102..1807+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
AGTGCAACAATGGCTCATACCATGGAGCCCGCCAGAACGGCTTCTAAGGA
CGAAGCTACCTGGACACCAAGCACAAGGGATCGGGGTATTGTAGACAGTA
TGGTCCACAATCGAAGCACGGAAACGCCTGATGTTGTAGACGTGACAGGC
TCGGAGACTTTATCTGAGACAGCAACTGCTGAACAAGAGAGGAAACGGAA
GAGGTATTACGGAGTTGTAGATTTAACAGAGTCGGGAGTATTCTCGGAAA
TGGCCCCTTTCGAGAGTAAGGGGAAAGGCAAGGGCAAGCGTGATGTTGTA
GACGTTGAAGCAGCATGGGAGCAATATGCGCAGCTCTCTGCTATTATGAA
TAAAGCAAAAGGCAGGATCGCTATGCAGTGTCCAATATGCAATGAACGAA
GGACGAAGGGGGTAGAGTGCTCTGAGGGACACTTTGTGTGCAAAACGTGC
TGTGTCCAGTACGTGTCTCTAACGTTAGAAGGAACTATTTTTTGGGATAC
AATTGAGTGTGTCAATTACCCATGCAAGTCCTATTACCGGGGGGTAGAAA
TCCGCGAGGTCATGCCTCAGAGCGCCATCCAGAGGATAGAGAAAAGACAA
ATGGACGCGGCTTACCTCGTTGCAGGAGAACGCGACCCTTCGTCACAGAC
GTTGATCGACAAGCACAGCAAGCCTTGCCCGAGGTGTGGAATTCCTATTC
AAAAAAACGGAGGGTGCGATCATGTCAAGTGCACGATGTGTAGGAAGGAC
TTCTGGTGGACATGTAACTGTGAGTACTCGGGCCAGCACAAGACTGGGTG
TGCTGCTTGGAGGAGGTAAGAATGCTTCCCCCGGAAAGTCTGCAGCAAAA
TATATTGCACACGAAAGTACCGAACAGAGGTGATCGGTTCGAAAATAAAC
AAGAGCATTCCCAAGGCTTGATGTGCAATGTAAATATTCCAACTTCGTTT
GTTAGGTATTTGCCGTAGAAGCAGCTTGCGACTGTGCTTCGACAGCAAGT
GGTAGTGTATTTGTAATTAGTCATGGGCTTCGCAGGGGTCGTTACGTAAT
AGAGAAGCTATCGAGAGGGGAGATTACAAGGTGCCTGCAGACTTACATTG
GCCGAACTTAGTTACAGCTGGCCAACGGAATACTGGGTGGAGAATCGCGA
AAGAGTTTTTGTATCCATAGCTCTACCTGTGAGGACTGCATATTATCTAG
CATCTAGAGGACTCCACTCTCCTTTCGTCCTTGCTTCAGTCGGTATTTTG
TTCAAGCGAGAATGTCTCCCTTGTGATGTTGGGAGCTTGAAGAAGTTGGA
GCCTGGAAGGATTCGTGGAATGAGGAGAACCCAAACATCCGTAATGGTGT
GCGTTTTTGCTCCGTGTCTTAGAAAGGCGGGCGTGGAGTGTGGAAGGCTG
TGCAGAGCTTACAAGTAACCGCCATTAATCAACCTATTGACCGACGTCAT
TACTGGTTTCTAATTTGCTGTAGAAGTATCAAAGTTGCGATTTTTGCCCT
GTGTTGATTCCGCAAGAGATTATCGCAGTTGCAAATTCGAACGATAGGGT
GTCAGCATCCCTCAACACGTATTCGAAGAGCGTTGTGATGAGCGACGCAG
ATGTATTCCTTATGTAGAAAGCGAAAACTTCTTATATGAGTATGGCTGTG
TAAGGAATGCAGCGTGCCACGAAATACGAAGAGACCATAGAGGAGAGAGG
GGGGCG
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Coding sequence (CDS) from alignment at H-paniculata_contig32122:102..1807+

>mRNA_H-paniculata_contig32122.8649.1 ID=mRNA_H-paniculata_contig32122.8649.1|Name=mRNA_H-paniculata_contig32122.8649.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=1620bp|location=Sequence derived from alignment at H-paniculata_contig32122:102..1807+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
ATGGCTCATACCATGGAGCCCGCCAGAACGGCTTCTAAGGACGAAGCTAC
CTGGACACCAAGCACAAGGGATCGGGGTATTGTAGACAGTATGGTCCACA
ATCGAAGCACGGAAACGCCTGATGTTGTAGACGTGACAGGCTCGGAGACT
TTATCTGAGACAGCAACTGCTGAACAAGAGAGGAAACGGAAGAGGTATTA
CGGAGTTGTAGATTTAACAGAGTCGGGAGTATTCTCGGAAATGGCCCCTT
TCGAGAGTAAGGGGAAAGGCAAGGGCAAGCGTGATGTTGTAGACGTTGAA
GCAGCATGGGAGCAATATGCGCAGCTCTCTGCTATTATGAATAAAGCAAA
AGGCAGGATCGCTATGCAGTGTCCAATATGCAATGAACGAAGGACGAAGG
GGGTAGAGTGCTCTGAGGGACACTTTGTGTGCAAAACGTGCTGTGTCCAG
TACGTGTCTCTAACGTTAGAAGGAACTATTTTTTGGGATACAATTGAGTG
TGTCAATTACCCATGCAAGTCCTATTACCGGGGGGTAGAAATCCGCGAGG
TCATGCCTCAGAGCGCCATCCAGAGGATAGAGAAAAGACAAATGGACGCG
GCTTACCTCGTTGCAGGAGAACGCGACCCTTCGTCACAGACGTTGATCGA
CAAGCACAGCAAGCCTTGCCCGAGGTGTGGAATTCCTATTCAAAAAAACG
GAGGGTGCGATCATGTCAAGTGCACGATGTGTAGGAAGGACTTCTGGTGG
ACATGTAACTGTGAGTACTCGGGCCAGCACAAGACTGGGTGTGCTGCTTG
GAGGAGGTAAATGGCTCATACCATGGAGCCCGCCAGAACGGCTTCTAAGG
ACGAAGCTACCTGGACACCAAGCACAAGGGATCGGGGTATTGTAGACAGT
ATGGTCCACAATCGAAGCACGGAAACGCCTGATGTTGTAGACGTGACAGG
CTCGGAGACTTTATCTGAGACAGCAACTGCTGAACAAGAGAGGAAACGGA
AGAGGTATTACGGAGTTGTAGATTTAACAGAGTCGGGAGTATTCTCGGAA
ATGGCCCCTTTCGAGAGTAAGGGGAAAGGCAAGGGCAAGCGTGATGTTGT
AGACGTTGAAGCAGCATGGGAGCAATATGCGCAGCTCTCTGCTATTATGA
ATAAAGCAAAAGGCAGGATCGCTATGCAGTGTCCAATATGCAATGAACGA
AGGACGAAGGGGGTAGAGTGCTCTGAGGGACACTTTGTGTGCAAAACGTG
CTGTGTCCAGTACGTGTCTCTAACGTTAGAAGGAACTATTTTTTGGGATA
CAATTGAGTGTGTCAATTACCCATGCAAGTCCTATTACCGGGGGGTAGAA
ATCCGCGAGGTCATGCCTCAGAGCGCCATCCAGAGGATAGAGAAAAGACA
AATGGACGCGGCTTACCTCGTTGCAGGAGAACGCGACCCTTCGTCACAGA
CGTTGATCGACAAGCACAGCAAGCCTTGCCCGAGGTGTGGAATTCCTATT
CAAAAAAACGGAGGGTGCGATCATGTCAAGTGCACGATGTGTAGGAAGGA
CTTCTGGTGGACATGTAACTGTGAGTACTCGGGCCAGCACAAGACTGGGT
GTGCTGCTTGGAGGAGGTAA
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