mRNA_H-paniculata_contig15150.3047.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig15150.3047.1
Unique NamemRNA_H-paniculata_contig15150.3047.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: A0A6H5KRZ7_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KRZ7_9PHAE)

HSP 1 Score: 155 bits (392), Expect = 9.660e-43
Identity = 75/97 (77.32%), Postives = 88/97 (90.72%), Query Frame = 1
Query:    1 QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAPTDVEELEKII 291
            +VL+TTNVL+RGVDVPAVSLVVNYDMPT+G   +PDPDTYLHRIGRTGRFGRRGVAINLI+D S+F +++ I+RHF+P  SM+RQA TDVEELEKII
Sbjct:  446 KVLLTTNVLSRGVDVPAVSLVVNYDMPTMGYSQDPDPDTYLHRIGRTGRFGRRGVAINLIQDDSTFRVMESIDRHFSPTGSMLRQASTDVEELEKII 542          
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: A0A835ZGD2_9STRA (ATP-dependent RNA helicase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZGD2_9STRA)

HSP 1 Score: 141 bits (356), Expect = 1.140e-38
Identity = 64/97 (65.98%), Postives = 85/97 (87.63%), Query Frame = 1
Query:    1 QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAPTDVEELEKII 291
            +VLITTNVLARGVDVPAV+LVVNYDMP LGR  +PD ++YLHRIGRTGRFGR+GVAIN ++D+ ++ +L++IERHF+P + M+R+AP D+EEL  ++
Sbjct:  281 KVLITTNVLARGVDVPAVTLVVNYDMPYLGRSKDPDYESYLHRIGRTGRFGRKGVAINFVQDNDAYSVLEQIERHFSPSKQMIRRAPDDMEELTNLV 377          
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: A0A7S2UVW1_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UVW1_9STRA)

HSP 1 Score: 121 bits (304), Expect = 3.350e-30
Identity = 62/98 (63.27%), Postives = 76/98 (77.55%), Query Frame = 1
Query:    1 QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAP-TDVEELEKII 291
            +VL+TTNVLARGVDVP+VSLV+NYD+P   +    D +TYLHRIGRTGRFGR+G AIN + D     IL +IERHF+PG  M+ QAP  DVEELE++I
Sbjct:  490 KVLVTTNVLARGVDVPSVSLVINYDLPVTYQ-NRADFETYLHRIGRTGRFGRKGCAINFVNDDQDQAILADIERHFSPGREMILQAPGDDVEELERVI 586          
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: A0A6V2XVD0_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V2XVD0_HETAK)

HSP 1 Score: 121 bits (303), Expect = 3.570e-30
Identity = 62/98 (63.27%), Postives = 80/98 (81.63%), Query Frame = 1
Query:    1 QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAP-TDVEELEKII 291
            +VLI+TNVLARGVDVPAVSLVVNYD+PT    G+PD ++YLHRIGRTGRFGR+GVAIN +++     +L++I  HF+P  +M+ +AP  D+EELEKII
Sbjct:  442 KVLISTNVLARGVDVPAVSLVVNYDIPTT-YDGQPDFESYLHRIGRTGRFGRKGVAINFVDNEHDKGLLEQIANHFSPARTMIFEAPGDDIEELEKII 538          
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: A0A7S1H2K6_9STRA (Hypothetical protein n=1 Tax=Thalassionema nitzschioides TaxID=33649 RepID=A0A7S1H2K6_9STRA)

HSP 1 Score: 113 bits (282), Expect = 3.940e-30
Identity = 58/97 (59.79%), Postives = 71/97 (73.20%), Query Frame = 1
Query:    1 QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAPTDVEELEKII 291
             VLITTN+LARGVDV  V LVVNYD+P    GG PD +TYLHRIGRTGRFGR+G AINLI D  +  +L  IE HF+ G+ M+  AP D E+L +++
Sbjct:   44 NVLITTNLLARGVDVDNVCLVVNYDVPVDKNGG-PDFETYLHRIGRTGRFGRKGTAINLISDQRAIEVLAAIEAHFSQGKEMIHNAPPDPEQLAEML 139          
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: A0A7H9HZM7_9SACH (Uncharacterized protein n=3 Tax=Torulaspora TaxID=4948 RepID=A0A7H9HZM7_9SACH)

HSP 1 Score: 118 bits (295), Expect = 2.550e-29
Identity = 58/97 (59.79%), Postives = 76/97 (78.35%), Query Frame = 1
Query:    1 QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAPTDVEELEKII 291
            +VLITTNVLARG+D+P VS+VVNYD+PTL  GG+PDP TY+HRIGRTGRFGR+GVAI+ + D  S+ +L  I+++F   E M R    D +E+EKI+
Sbjct:  363 KVLITTNVLARGIDIPTVSMVVNYDLPTLP-GGQPDPSTYIHRIGRTGRFGRKGVAISFVHDKKSYNVLSSIQKYFGDIE-MTRVPTNDWDEVEKIV 457          
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: A0A7S1BB19_9STRA (Hypothetical protein n=2 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1BB19_9STRA)

HSP 1 Score: 117 bits (292), Expect = 7.220e-29
Identity = 59/93 (63.44%), Postives = 73/93 (78.49%), Query Frame = 1
Query:    1 QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAPTDVEEL 279
             VLITTNVLARGVDV  V +V+NYD+P + R GEPD +TYLHRIGRTGRFG++G AI+LI D  S  IL  IE HF+PG+ M+++A +D EEL
Sbjct:  372 NVLITTNVLARGVDVDNVCMVINYDVP-MTRNGEPDFETYLHRIGRTGRFGKKGSAISLIADEKSLKILAAIEAHFSPGKEMIQKASSDPEEL 463          
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: A0A125RE16_9SACH (HBR249Wp n=1 Tax=Eremothecium sinecaudum TaxID=45286 RepID=A0A125RE16_9SACH)

HSP 1 Score: 116 bits (291), Expect = 1.090e-28
Identity = 58/98 (59.18%), Postives = 77/98 (78.57%), Query Frame = 1
Query:    1 QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAPT-DVEELEKII 291
            +VLITTNVLARG+D+P+VS+VVNYD+PT    G+PDP TY+HRIGRTGRFGR+GVAI+ I D  SF  L  I+++F  G+  +   PT D++E+EKI+
Sbjct:  379 KVLITTNVLARGIDIPSVSMVVNYDLPTTAN-GQPDPSTYVHRIGRTGRFGRKGVAISFIHDRKSFDTLAAIQKYF--GDIQITNVPTGDMDEMEKIV 473          
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: H0GNF2_SACCK (Dbp5p n=1 Tax=Saccharomyces cerevisiae x Saccharomyces kudriavzevii (strain VIN7) TaxID=1095631 RepID=H0GNF2_SACCK)

HSP 1 Score: 114 bits (285), Expect = 2.340e-28
Identity = 58/97 (59.79%), Postives = 75/97 (77.32%), Query Frame = 1
Query:    1 QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAPTDVEELEKII 291
            +VLITTNVLARG+D+P VS+VVNYD+PTL   G+ DP TY+HRIGRTGRFGR+GVAI+ + D +SF IL  I+++F   E M R    D +E+EKI+
Sbjct:  273 KVLITTNVLARGIDIPTVSMVVNYDLPTLAN-GQADPATYIHRIGRTGRFGRKGVAISFVHDKNSFNILSAIQKYFGDIE-MTRVPTDDWDEVEKIV 367          
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Match: A0A7R9UYV2_DIALT (Hypothetical protein n=1 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A7R9UYV2_DIALT)

HSP 1 Score: 113 bits (283), Expect = 3.030e-28
Identity = 55/97 (56.70%), Postives = 77/97 (79.38%), Query Frame = 1
Query:    7 LITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRFGRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAP--TDVEELEKII 291
            L+TTNVL+RG+DVPAV+LV+NYDMP + +GG  DPDTYLHR+GRTGRFGR+G A+N+I D    ++L++IE +F   + ++++ P  TD EE EKI+
Sbjct:  249 LVTTNVLSRGIDVPAVTLVINYDMP-VRKGGTADPDTYLHRVGRTGRFGRKGSALNMIHDEKEMVVLKQIEAYF-DRQGLIKEIPPDTDGEEFEKIL 343          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig15150.3047.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KRZ7_9PHAE9.660e-4377.32Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A835ZGD2_9STRA1.140e-3865.98ATP-dependent RNA helicase n=1 Tax=Tribonema minus... [more]
A0A7S2UVW1_9STRA3.350e-3063.27Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A6V2XVD0_HETAK3.570e-3063.27Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A7S1H2K6_9STRA3.940e-3059.79Hypothetical protein n=1 Tax=Thalassionema nitzsch... [more]
A0A7H9HZM7_9SACH2.550e-2959.79Uncharacterized protein n=3 Tax=Torulaspora TaxID=... [more]
A0A7S1BB19_9STRA7.220e-2963.44Hypothetical protein n=2 Tax=Corethron hystrix Tax... [more]
A0A125RE16_9SACH1.090e-2859.18HBR249Wp n=1 Tax=Eremothecium sinecaudum TaxID=452... [more]
H0GNF2_SACCK2.340e-2859.79Dbp5p n=1 Tax=Saccharomyces cerevisiae x Saccharom... [more]
A0A7R9UYV2_DIALT3.030e-2856.70Hypothetical protein n=1 Tax=Diacronema lutheri Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig15150contigH-paniculata_contig15150:136..1265 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score155.6
Seed ortholog evalue1.2e-35
Seed eggNOG ortholog2880.D7G332
Preferred nameDDX25
KEGG koko:K09097,ko:K13126,ko:K18655,ko:K18656
KEGG Pathwayko03013,ko03015,ko03018,ko04066,ko04934,ko05200,ko05204,ko05211,map03013,map03015,map03018,map04066,map04934,map05200,map05204,map05211
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000166,GO:0002181,GO:0002184,GO:0003006,GO:0003674,GO:0003676,GO:0003723,GO:0003724,GO:0003824,GO:0004004,GO:0004386,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005643,GO:0005681,GO:0005730,GO:0005737,GO:0005844,GO:0005933,GO:0005934,GO:0006139,GO:0006396,GO:0006403,GO:0006405,GO:0006406,GO:0006412,GO:0006415,GO:0006417,GO:0006518,GO:0006611,GO:0006725,GO:0006807,GO:0006810,GO:0006886,GO:0006913,GO:0007276,GO:0007281,GO:0007283,GO:0007286,GO:0008026,GO:0008104,GO:0008144,GO:0008150,GO:0008152,GO:0008186,GO:0008380,GO:0009058,GO:0009059,GO:0009889,GO:0009893,GO:0009987,GO:0010467,GO:0010468,GO:0010501,GO:0010556,GO:0010604,GO:0010608,GO:0010628,GO:0010941,GO:0010942,GO:0012505,GO:0015031,GO:0015833,GO:0015931,GO:0016020,GO:0016043,GO:0016070,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0016973,GO:0017076,GO:0017111,GO:0019222,GO:0019538,GO:0019953,GO:0022411,GO:0022412,GO:0022414,GO:0030154,GO:0030427,GO:0030554,GO:0030587,GO:0030684,GO:0030687,GO:0031090,GO:0031323,GO:0031326,GO:0031503,GO:0031965,GO:0031967,GO:0031974,GO:0031975,GO:0031981,GO:0032268,GO:0032501,GO:0032502,GO:0032504,GO:0032553,GO:0032555,GO:0032559,GO:0032984,GO:0032991,GO:0033036,GO:0033391,GO:0034248,GO:0034613,GO:0034641,GO:0034645,GO:0035639,GO:0035770,GO:0036094,GO:0036464,GO:0042623,GO:0042886,GO:0042981,GO:0043043,GO:0043065,GO:0043067,GO:0043068,GO:0043167,GO:0043168,GO:0043170,GO:0043186,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043603,GO:0043604,GO:0043624,GO:0043933,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044464,GO:0044614,GO:0044703,GO:0045184,GO:0045495,GO:0046483,GO:0046907,GO:0048232,GO:0048468,GO:0048515,GO:0048518,GO:0048522,GO:0048609,GO:0048856,GO:0048869,GO:0050657,GO:0050658,GO:0050789,GO:0050794,GO:0051028,GO:0051168,GO:0051169,GO:0051171,GO:0051179,GO:0051234,GO:0051236,GO:0051246,GO:0051641,GO:0051649,GO:0051703,GO:0051704,GO:0060255,GO:0060293,GO:0065007,GO:0070013,GO:0070035,GO:0070727,GO:0071013,GO:0071166,GO:0071426,GO:0071427,GO:0071702,GO:0071704,GO:0071705,GO:0071840,GO:0080090,GO:0090304,GO:0090702,GO:0097159,GO:0097367,GO:0099120,GO:0140098,GO:1901265,GO:1901360,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1902494,GO:1990904,GO:2000112
EggNOG free text desc.RNA helicase activity
EggNOG OGsKOG0332@1,KOG0332@2759
EC3.6.4.13
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03000,ko03019,ko03041
Exons2
Model size291
Cds size291
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929214.8628585-CDS-H-paniculata_contig15150:135..3181622929214.8628585-CDS-H-paniculata_contig15150:135..318Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig15150 136..318 +
1691480773.4622595-CDS-H-paniculata_contig15150:135..3181691480773.4622595-CDS-H-paniculata_contig15150:135..318Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig15150 136..318 +
1622929214.873229-CDS-H-paniculata_contig15150:1157..12651622929214.873229-CDS-H-paniculata_contig15150:1157..1265Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig15150 1158..1265 +
1691480773.4749599-CDS-H-paniculata_contig15150:1157..12651691480773.4749599-CDS-H-paniculata_contig15150:1157..1265Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig15150 1158..1265 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig15150.3047.1prot_H-paniculata_contig15150.3047.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig15150 136..1265 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig15150.3047.1

>prot_H-paniculata_contig15150.3047.1 ID=prot_H-paniculata_contig15150.3047.1|Name=mRNA_H-paniculata_contig15150.3047.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=97bp
QVLITTNVLARGVDVPAVSLVVNYDMPTLGRGGEPDPDTYLHRIGRTGRF
GRRGVAINLIEDHSSFMILQEIERHFAPGESMVRQAPTDVEELEKII
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mRNA from alignment at H-paniculata_contig15150:136..1265+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig15150.3047.1 ID=mRNA_H-paniculata_contig15150.3047.1|Name=mRNA_H-paniculata_contig15150.3047.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=1130bp|location=Sequence derived from alignment at H-paniculata_contig15150:136..1265+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
CAGGTCCTGATTACCACCAACGTGTTGGCGCGAGGAGTGGATGTTCCTGC CGTATCTCTGGTGGTCAATTACGATATGCCCACACTTGGACGAGGTGGTG AGCCTGATCCCGACACGTATTTGCACCGCATCGGCCGCACCGGGCGCTTC GGGCGGAGAGGCGTGGCCATCAATCTTATTGAAGTACGTCATTTTCCATT TCATGTTTGTGTGATTTTTTTCCGGCTGGAGGTGTTTGTGGAATGCAGAA TTGAAAATATAGGAAAAGAAAGCGTCTCTGTTATCGAAGCGCGCTGTCAT TTTCCAGCGAGGGTCGTGCTTTATCAGTTGCTTTATCATATTTTAAAGTG TGTGCTCAAGTGGCTAATAAAAAACGAATGTTCGTGCAAGGGCAGCAAGT GGATGTGTGTCATAGATTATGATAAAAGACAGATTGCACAGGCGTACTCG TGGCTTTATTACCAACAGCTCCATGTTGTGAATTCAAACAAACAAAAAAC GACCTTGTTCTCAAAGTTCGCCGTTGTTTTCCAAAAAGTGTATCGATATA TATATATATATATGTATAATATATTTACATGCATTCATCGGTGCCAATTA TATACTGTGTCGTGGACCTGAAACAAAAGAGAGAAAAAACCCGTCGAAGC TTTGTTTTCGTCGGGTTTGTTTTGTAGTTCAGTCATGATTTATGGTTCGG TGTGCTCATGTCTCGGCTGCACCCGCATCTATGTGTCCTTGCAAATGGGA TAAGAAAAGTAAAATATTTTCTAGCCATATTTGGAAAAACTTATGAGCCA CCAGAGATCGCTCTGATAACAGAGCTCCCTGTATCTCCCCCATCCCTAAG CTCTGCCCTCTCGAGGAGGTGTGGTCAATATTATACCCCCCCTTGGTTTT TTCATTTTTTCAGATTGTGGATAATAACTAAGTGATCCAGCAGCTTGGAG ATCCTGAATAGACTAGTACGTTGATTTTGTCTCATCCCCCTTCCCTCCCC CCCGCGCGCCTCCTTTTATCAGGACCATTCGTCGTTCATGATATTGCAAG AGATCGAGAGGCACTTCGCGCCGGGGGAGTCGATGGTGCGGCAGGCTCCC ACGGATGTGGAAGAGCTAGAGAAGATTATA
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Coding sequence (CDS) from alignment at H-paniculata_contig15150:136..1265+

>mRNA_H-paniculata_contig15150.3047.1 ID=mRNA_H-paniculata_contig15150.3047.1|Name=mRNA_H-paniculata_contig15150.3047.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=582bp|location=Sequence derived from alignment at H-paniculata_contig15150:136..1265+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
CAGGTCCTGATTACCACCAACGTGTTGGCGCGAGGAGTGGATGTTCCTGC
CGTATCTCTGGTGGTCAATTACGATATGCCCACACTTGGACGAGGTGGTG
AGCCTGATCCCGACACGTATTTGCACCGCATCGGCCGCACCGGGCGCTTC
GGGCGGAGAGGCGTGGCCATCAATCTTATTGAACAGGTCCTGATTACCAC
CAACGTGTTGGCGCGAGGAGTGGATGTTCCTGCCGTATCTCTGGTGGTCA
ATTACGATATGCCCACACTTGGACGAGGTGGTGAGCCTGATCCCGACACG
TATTTGCACCGCATCGGCCGCACCGGGCGCTTCGGGCGGAGAGGCGTGGC
CATCAATCTTATTGAAGACCATTCGTCGTTCATGATATTGCAAGAGATCG
AGAGGCACTTCGCGCCGGGGGAGTCGATGGTGCGGCAGGCTCCCACGGAT
GTGGAAGAGCTAGAGAAGATTATAGACCATTCGTCGTTCATGATATTGCA
AGAGATCGAGAGGCACTTCGCGCCGGGGGAGTCGATGGTGCGGCAGGCTC
CCACGGATGTGGAAGAGCTAGAGAAGATTATA
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