mRNA_H-paniculata_contig1432.2610.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: D8LP23_ECTSI (Thiamine monophosphate synthase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LP23_ECTSI)

HSP 1 Score: 746 bits (1927), Expect = 1.690e-262
Identity = 395/538 (73.42%), Postives = 449/538 (83.46%), Query Frame = 2

Query:  224 PIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRYKGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHSDWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAG--RLWVNDFWREAIKHGAYGVHLGQEDLEAGGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAVKHWNAVFATKAK 1831
            PIVWTIAGSDSGGGAGIQADLH++H+LG HGCSVITAMTAQNSHAVTHVEYASVEMI+ATI AL+ DLP A VKLGMMGTDAVV  VG FL+ YKG VVCDPVM+STSG+ L+ EGAE  M ER+FPRATMITPNL+EAE L+GR LRTP +VEAGAA LLASS AGG+LIKGGH+ +        E E G       GA       + S+ + +SQDYWTDGT GG+FWLT PRV++++THGTGCTLSSAAAACLA G +P DSVV+AKAYVTQGIRVA+R GKG PGPV  T WPSR+DCFPWVT TAE GAG GRPEAF  CH DWG+YPVVETAEWV++LL+LGV+DIQLR+KGASPEALDAE++ AA ACR S A  G G  RLWVNDFWR A++HGAYGVHLGQEDLEAGGGEAL+AI+ AGLRLG+STHSYLELARATAVRPSYISLGPVFET SKKVAFSPRGA LV  WRALVD  +PLIAIGGI+L  AP+++EAGADSIAVISA+TKA+DVE+AV+ W+  F  + +
Sbjct:   55 PIVWTIAGSDSGGGAGIQADLHAMHSLGVHGCSVITAMTAQNSHAVTHVEYASVEMIQATIDALQSDLPPAAVKLGMMGTDAVVSVVGAFLEGYKGNVVCDPVMVSTSGSRLLPEGAEALMKERVFPRATMITPNLIEAEVLLGRSLRTPADVEAGAASLLASSAAGGVLIKGGHSED--------EREPG-------GAD------RPSARQRYSQDYWTDGTPGGSFWLTTPRVDSDDTHGTGCTLSSAAAACLAKGLDPADSVVLAKAYVTQGIRVARRFGKG-PGPVAHTGWPSRADCFPWVTATAEGGAG-GRPEAFPKCHDDWGLYPVVETAEWVEKLLSLGVRDIQLRVKGASPEALDAEVARAAQACRDSAAGAGGGGGRLWVNDFWRAAVRHGAYGVHLGQEDLEAGGGEALSAISKAGLRLGLSTHSYLELARATAVRPSYISLGPVFETTSKKVAFSPRGAVLVGAWRALVD--VPLIAIGGISLERAPEVLEAGADSIAVISAITKADDVEEAVRQWDTAFEARRR 567          

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: A0A835ZKL9_9STRA (Thiamine monophosphate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZKL9_9STRA)

HSP 1 Score: 538 bits (1387), Expect = 4.640e-182
Identity = 308/534 (57.68%), Postives = 371/534 (69.48%), Query Frame = 2

Query:  221 PPIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRYKGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHSDWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAGRLWVNDFWREAIKHGAYGVHLGQEDL-EAGGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAVKHWNAVFA 1819
            P I WTIAGSDSGGGAGIQADL ++H LGAHGCSVITAMTAQNS  VT VEYAS EMIKA++ AL  DLPA  +KLGM+GT+ V+  V  FL  Y+G VVCDPVMISTSG+ LM   A+ ++   IFPR+T+ITPN +EAEAL+GR LR+  +VE  A +LL S+G   +LIKGGH  E                                 S   +QD+WTDG      WL++PR  + +THGTGCTLS+A AACLA G++  D+V + KAYVT GI  A+RLG  GPGPV  T WP++    PW+T TAE+G    RPE   PCH DWG+YPVV++AEWV RL+ +GV+D+QLR+KG S  AL+ EI+ A    +  +AA GA RLWVNDFWREA+  GAYGVH+GQEDL E G  E   A+AAAG RLG+STHSY ELARA AVRPSYISLGPVF T SK V F+PRGA  VR WR L+   +PLIAIGGITL  AP ++ AGA+ IAVI+AVTKA DV  AV  W A++A
Sbjct:    5 PAIAWTIAGSDSGGGAGIQADLLAMHNLGAHGCSVITAMTAQNSKGVTKVEYASCEMIKASLEALACDLPAGAIKLGMLGTEEVMAEVASFLGTYEGPVVCDPVMISTSGSSLMDPAAKAKLTAAIFPRSTIITPNKLEAEALLGRELRSARDVERAARDLL-STGVRSVLIKGGHHDE---------------------------------SGALAQDFWTDGERA--LWLSSPRARHADTHGTGCTLSAAIAACLAQGYDACDAVTIGKAYVTAGIGAARRLG-AGPGPVAHTGWPAQHAAMPWLTETAEQGHD--RPE-LPPCHRDWGLYPVVDSAEWVGRLVGMGVRDVQLRVKGMSGGALEGEIARA----QELSAAAGA-RLWVNDFWREAVACGAYGVHMGQEDLQEQGPFEVGFALAAAGTRLGISTHSYAELARALAVRPSYISLGPVFATASKDVQFAPRGAEEVRRWRNLIPPHVPLIAIGGITLERAPGVLAAGAEGIAVIAAVTKAPDVASAVAEWAALWA 493          

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: W7TKQ7_9STRA (Hydroxymethylpyrimidine kinase phosphomethylpyrimidine kinase thiamine-phosphate diphosphorylase n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TKQ7_9STRA)

HSP 1 Score: 495 bits (1274), Expect = 9.710e-164
Identity = 284/543 (52.30%), Postives = 360/543 (66.30%), Query Frame = 2

Query:  218 VPPIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRY-----KGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHSDWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAGRLWVNDFWREAIKHGAYGVHLGQEDLEA---GGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAVKHWNAVFAT 1822
            VPPIVW+IAGSDSGGGAG+QADL + HALG HGCSVITA+TAQNSH V  VE +S++M++ T+A LE+D+PA  +K+GM+ T  VV AVG FL         G V+CDPVM+++SG+ L  E A   M++ IFPRAT+ITPN  EAE L+G  LRTP ++E  A +LLA   +G +L+KGGHA + L     GE         D   A               QDY  +       W+T+PR+EN NTHGTGCTLSSA AA LA      D+VV+AKAYV+QGIR A++LG+G PGPV QT WP+ +  FPWVT   EEG+   RP  F+ C  D G+Y  V +A WV+RLL LGVKDIQLRIK      L+ E++ AA +CR + A     RLWVND+W  A+++ AYG+H+GQED++         L A+   G+RLGVSTH++ EL RA A+RPSYISLGP+F T SKKV F P+G   +R WR LVD  +PL+AIGGI L  A  + +AGAD I VISA+T+A DV QAV+ W AV  T
Sbjct:   69 VPPIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGL----GGERSASGEGLFDAAVA---------------QDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRG-PGPVQQTGWPASASDFPWVTSRVEEGSKK-RPGPFAECERDMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANA-----RLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLVD--VPLVAIGGIGLEAAQGVRDAGADGICVISALTQASDVAQAVQAWRAVLRT 583          

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: A0A8A4TWK8_9BACT (Thiamine-phosphate synthase n=1 Tax=Sulfidibacter corallicola TaxID=2818388 RepID=A0A8A4TWK8_9BACT)

HSP 1 Score: 428 bits (1100), Expect = 7.130e-139
Identity = 262/532 (49.25%), Postives = 328/532 (61.65%), Query Frame = 2

Query:  224 PIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRYKGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHS-DWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAGRLWVNDFWREAIKHGAYGVHLGQEDLEAGGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAVKHWNAVF 1816
            P+ WTIAGSDSGGGAGIQADL +  ALG HGCSVITA+TAQN+  V  V      M+     AL EDLPAA +KLGM+G  AV+ AV E L++    VV DPVM++TSG+ LMA  A   MVE +FPRA +ITPNL EAEAL+GRRLRTP +VE  A +L A  GA  +LIKGGH                                       FSQD+++DGT   +FWLT PR+E  +THG+GCTLS+A AA +ALG+   D++V+ KA++T  IR A+  G G PGPV+Q AWP R    PW+T  AE G    R   F  C     G YP+V+   W+KRLL LGV  IQLRIK  +  AL+ EI  A    +         RL+VND+W  A++  AYGVHLGQEDL       LA +  AGLRLGVSTH Y E+ARA AV PSY+++GPVF T +K + F+P+G   +  WR  +    PL+AIGGI L    +++ AGADSIAVI+A+T+AED E A + W   F
Sbjct:   20 PVCWTIAGSDSGGGAGIQADLKTFFALGVHGCSVITALTAQNTRGVAAVALTEPSMLDYQWQALVEDLPAAAIKLGMLGDAAVIRAVAERLNQTDAFVVLDPVMVATSGSSLMAPEARSVMVETLFPRADLITPNLPEAEALLGRRLRTPAQVEHAAVQLRAL-GARAVLIKGGH-----------------------------------GDAPFSQDHFSDGTA--SFWLTGPRIETPHTHGSGCTLSAAIAAAVALGYELADALVIGKAFITAAIRDARPQGSG-PGPVLQGAWPEREADLPWMTSDAESGRNRLR---FPDCGPRQLGFYPIVDRVAWMKRLLPLGVSTIQLRIKDLTGAALENEIHRAVELSKHYNC-----RLFVNDYWELALRFDAYGVHLGQEDLPHAD---LARLCKAGLRLGVSTHCYYEVARALAVHPSYMAIGPVFSTQTKVMRFAPQGLAALARWRRSLG--FPLVAIGGIKLNHVDRVLAAGADSIAVITAITEAEDPEGAARQWLQCF 499          

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: A0A7T4R1J5_9GAMM (Bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase n=1 Tax=Spongiibacter nanhainus TaxID=2794344 RepID=A0A7T4R1J5_9GAMM)

HSP 1 Score: 422 bits (1085), Expect = 1.060e-136
Identity = 255/532 (47.93%), Postives = 333/532 (62.59%), Query Frame = 2

Query:  224 PIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRYKGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHSDWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAGRLWVNDFWREAIKHGAYGVHLGQEDLEAGGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAVKHWNAVFA 1819
            PI W+IAGSDSGGGAGIQADL + H  G HGC+VITA+TAQNS AV  V       + A I AL+ DL A  +KLGM+G+  VV  V ++L+ Y+G VVCDPVM+STSG  LM +G    +VE++ PRA ++TPNL EA+ L+GR +    +VE  AA+++A  GA  +L+ GGH + R                               S R    DYWTDG  G  FWL+ P ++  NTHGTGCTLSSA AA +A GF+  D++V+AKAYV+QG+R+A +LG GGPGPV    WP R   FP ++    + A +  P AF+ C    G+YPVVETA+WV+ LL  GVK +QLR K    E L+AEI  A A  R   A     R+++ND W++AI  GAYGVHLGQ DL++     LAAIAAAGLRLGVSTH Y E+ARA  +RPSYI++GP+F T SK + F P+G   +R W  L+     L AIGGI    AP+++  G  S A+I+A+T A+D  Q V+   ++ A
Sbjct:    8 PIAWSIAGSDSGGGAGIQADLATFHDFGVHGCTVITAITAQNSFAVGQVSMTPRRTMAAQINALDSDLQADAIKLGMLGSTEVVEMVAKYLEDYRGFVVCDPVMVSTSGGELMEDGVSEVLVEKLLPRADLLTPNLSEAQILLGREINGLEQVERAAADIVAM-GARSVLLTGGHLSTR------------------------------HSQR---HDYWTDGQDG--FWLSGPNIDTLNTHGTGCTLSSAIAALMAKGFDLRDALVIAKAYVSQGLRMAVQLG-GGPGPVGHVGWPQRLADFPCIS---RDLASSVTP-AFASCDGPLGLYPVVETADWVETLLQWGVKTVQLRAKDLQGERLEAEIQRAVALGREHQA-----RVFINDHWQQAIAAGAYGVHLGQGDLDSAD---LAAIAAAGLRLGVSTHCYYEVARAHGLRPSYIAIGPIFPTTSKDMPFGPQGVNQLRRWVNLLGPHYTLTAIGGIDGKRAPEVLSTGVGSCAMITAITLADDPRQIVEQLMSLHA 490          

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: A0A2D7WK18_9GAMM (Phosphomethylpyrimidine kinase n=3 Tax=Spongiibacter TaxID=630749 RepID=A0A2D7WK18_9GAMM)

HSP 1 Score: 421 bits (1082), Expect = 2.370e-136
Identity = 248/524 (47.33%), Postives = 325/524 (62.02%), Query Frame = 2

Query:  224 PIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRYKGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHSDWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAGRLWVNDFWREAIKHGAYGVHLGQEDLEAGGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAV 1795
            PI WTIAGSDSGGGAGIQADL S H  G HGCSV+TA+TAQNS  V HV       + A I AL+ DL AA +KLGM+G + VV  V ++L  Y+G VVCDPVM+STSG  LM +G    ++E+I PRA ++TPN+ EAE L+ R + +P +VE  A +++   GA  +L+ GGH +                   D G                  DYWTDG  G  FWL+   ++  NTHGTGCTLSSA AA +A G+   D++V+AKAYV+QG+R+A +LG GGPGPVV   WP R + FP ++        A   +AF  C  + G+YPVV++ EW++RLL  GVK IQLRIK      LD EI  A A  +  +A     R+++ND+WR+AI  GAYGVHLGQEDL+      + AIAAAGLRLGVSTHSY E+ARA A+ PSYI++GPV+ T SK + F  +G   ++ W  ++     L AIGGI    AP ++  G  S A+I+A+T+A+D E  V
Sbjct:    7 PIAWTIAGSDSGGGAGIQADLASFHDFGVHGCSVVTALTAQNSFTVGHVAITPRRALAAQINALDSDLQAAAIKLGMLGGEDVVEMVAKYLADYRGFVVCDPVMVSTSGGQLMEDGVSEALIEKILPRADLLTPNISEAETLLNREINSPADVERAAQDIVVL-GARSVLLTGGHLS------------------TDDGCR---------------HDYWTDGESG--FWLSGENIDTLNTHGTGCTLSSAIAALIARGYELADALVIAKAYVSQGLRLAVQLG-GGPGPVVHLGWPQRLEDFPRLSRQLP----AAPIQAFPDCGGELGLYPVVDSTEWLERLLRAGVKTIQLRIKNPEQSNLDEEIRRAVALGQQYSA-----RVFINDYWRQAIDAGAYGVHLGQEDLDIAD---IGAIAAAGLRLGVSTHSYYEIARAHALSPSYIAIGPVYPTTSKVMRFGAQGLTQLKRWVDILSPSYTLTAIGGIDRRRAPDVLATGVGSCAMITAITEADDPEAVV 481          

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: A0A0F7M0F2_9GAMM (Thiamine-phosphate synthase n=1 Tax=Spongiibacter sp. IMCC21906 TaxID=1620392 RepID=A0A0F7M0F2_9GAMM)

HSP 1 Score: 418 bits (1075), Expect = 3.200e-135
Identity = 251/524 (47.90%), Postives = 328/524 (62.60%), Query Frame = 2

Query:  224 PIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRYKGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHSDWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAGRLWVNDFWREAIKHGAYGVHLGQEDLEAGGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAV 1795
            PIVW+IAGSDSGGGAGIQADL + H  G HGC+VITA+TAQNS  V HV       + A I AL+ DL A  +KLGM+G   VV  V ++L+ Y G VVCDPVM+STSG  LM  G    ++E++ PRA ++TPNL EAEAL+ R++    EVE  AA+++A  GA  +LI GGH + R        N+                            DYWTDG  G  FWL+   ++  NTHGTGCTLSSA AA +A  F+  D++V+AKAYV+QG+R++ +LG GGPGPV    WP R   FP V+        AG+  AF  C    G+YPVV++AEW+ RLL  GVK IQLR+K  S   L+ EI+ A A  +   A     R+++ND+W++A+  GAYGVHLGQEDL+   G  L AIA AGLRLGVSTHSY E+ARA  +RPSYI++GP++ T SK++ FSP+G   ++ W  ++     L AIGGI +  A  ++  G  S A+ISA+T+AED E  V
Sbjct:    7 PIVWSIAGSDSGGGAGIQADLATFHDFGVHGCTVITAITAQNSFTVGHVSTTPRRALAAQINALDSDLQADAIKLGMLGGVDVVKMVAKYLEDYPGFVVCDPVMVSTSGGELMESGVAEVLIEQLLPRADLLTPNLSEAEALLNRKIDGLEEVERAAADIVAL-GARSVLITGGHLSAR-------NNQ--------------------------RHDYWTDGLNG--FWLSGQNIDTLNTHGTGCTLSSAIAALMARDFDLKDALVLAKAYVSQGLRMSVQLG-GGPGPVAHVGWPQRLADFPGVSRRLP---AAGKVLAFPACDGPLGLYPVVDSAEWIARLLKCGVKTIQLRVKEVSGSDLEKEITEAVALGQQYNA-----RVFINDYWQQAVAAGAYGVHLGQEDLD---GTDLHAIAEAGLRLGVSTHSYHEVARAHGIRPSYIAIGPIYPTTSKQMPFSPQGVAKLQRWVNILAPHYTLTAIGGIDVKRADAVLATGVGSAAMISAITQAEDPEAVV 482          

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: A0A2E9SND5_9GAMM (Thiamine-phosphate synthase n=2 Tax=Spongiibacter TaxID=630749 RepID=A0A2E9SND5_9GAMM)

HSP 1 Score: 417 bits (1071), Expect = 1.110e-134
Identity = 249/524 (47.52%), Postives = 326/524 (62.21%), Query Frame = 2

Query:  224 PIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRYKGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHSDWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAGRLWVNDFWREAIKHGAYGVHLGQEDLEAGGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAV 1795
            PI W+IAGSDSGGGAGIQADL + H  G HGC+VITA+TAQNS AV HV   S   + A I AL+ DLPA  +KLGM+G+  VV  V ++L  Y G VVCDPVM+STSG  LM  G    +VE++ PRA ++TPN+ EAE L+ R++ T  EVE  A +++   GA  +L+ GGH   R                       D +            DYWTDG  G  FWL+ P ++  NTHGTGCTLSSA AA LA G+   D++V+AKAYV+QG+R++ +LG GGPGPV    WP +   +P ++    +     +P AF  C  + G+YPVV+++EWV+RLL  GVK IQLRIK      LD EI  A    R   A     R+++ND+W +AI  GAYGVHLGQEDL+      + AIA AGLRLGVSTHS+ E+ARA A+RPSYI++GPV+ T SK++ F P+G   +R W  L+     L AIGGI L  A +++  G  S A+I+A+T+A+D E  V
Sbjct:    7 PIAWSIAGSDSGGGAGIQADLATFHDFGVHGCTVITAITAQNSFAVGHVAVTSHRALAAQINALDSDLPAGAIKLGMLGSAEVVEVVAKYLQDYPGPVVCDPVMVSTSGGLLMENGVSQVLVEKLLPRADLLTPNISEAETLLDRKITTAEEVERAAQDIVVL-GARSVLLTGGHLVTR-----------------------DGL----------CHDYWTDGEQG--FWLSGPCIDTLNTHGTGCTLSSAIAALLARGYEMADALVLAKAYVSQGLRLSVQLG-GGPGPVTHMGWPKQLCDYPGLSRRFPKA----KP-AFPACDGELGLYPVVDSSEWVERLLKSGVKTIQLRIKDPHQPNLDEEIQRAVTLGREYQA-----RVFINDYWPQAITAGAYGVHLGQEDLDVAD---IQAIADAGLRLGVSTHSHYEVARAHALRPSYIAIGPVYPTTSKEMRFGPQGVNRLRHWVELLSPGYTLTAIGGINLKRADEVLATGVGSCAMITAITEADDPESVV 480          

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: I2JG64_9GAMM (Thiamine-phosphate synthase n=3 Tax=Spongiibacteraceae TaxID=1706375 RepID=I2JG64_9GAMM)

HSP 1 Score: 417 bits (1071), Expect = 1.310e-134
Identity = 249/531 (46.89%), Postives = 334/531 (62.90%), Query Frame = 2

Query:  224 PIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRYKGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHSDWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAGRLWVNDFWREAIKHGAYGVHLGQEDLEAGGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAVKHWNAVF 1816
            PIVW+IAGSDSGGGAGIQADL + H  G HGC+VITA+TAQNS AV HV       + A I AL+ DL A V+KLGM+  + VV  V ++LD Y+G VVCDPV+ S++G  L        ++E++ PR  ++TPNL EAE+++ RR++   +VE  AA+++A  GA  +LIKGGH        TA  N+                            DYWTDG  G  FWL+   V+  NTHGTGCTLSSA AA LA G+   D++V+AKAYV+QG+R+  +LG GGPGPV    WP +   FP ++    +   +G    F  C  + G+YPVV++A+W+KRLLA GVK IQLR+KG   EAL  E++ A A  R  +A     R+++NDFW+ AI  GAYGVHLGQEDL+    EA   IA+AGLRLGVSTH+Y E+ARA  ++PSYI++GPV+ T SK + +SP+G   +R W  ++     L AIGGI +  A +++  G  SIA+ISA+T+AE+ E  V    A+ 
Sbjct:    7 PIVWSIAGSDSGGGAGIQADLATFHDFGVHGCTVITAITAQNSFAVGHVSVTPRRTLAAQINALDSDLQADVIKLGMLADEGVVGMVAKYLDSYRGFVVCDPVLSSSTGGDLTDGNLSACLIEKLLPRCDLLTPNLSEAESILNRRIKGLDDVERAAADIVAL-GARAVLIKGGHL-------TARNNQ--------------------------RHDYWTDGIDG--FWLSGANVDTLNTHGTGCTLSSAIAASLARGYALPDALVIAKAYVSQGLRMGVQLG-GGPGPVAHVGWPQQLIDFPSLSRRLPQ---SGPRPVFPSCEGELGLYPVVDSADWIKRLLACGVKTIQLRVKGLQGEALRKEVARAVALGREYSA-----RVFINDFWQLAIAEGAYGVHLGQEDLDIADIEA---IASAGLRLGVSTHTYYEVARAHGLQPSYIAIGPVYRTTSKDMPYSPQGLMQLRRWVGVLGDNYTLTAIGGIDIKRAKEVLLTGVKSIAMISAITQAENPEAVVAELMAMI 489          

BLAST of mRNA_H-paniculata_contig1432.2610.1 vs. uniprot
Match: UPI001C56C5F7 (bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase n=1 Tax=Zhongshania sp. CAU 1632 TaxID=2857107 RepID=UPI001C56C5F7)

HSP 1 Score: 410 bits (1055), Expect = 3.010e-132
Identity = 247/535 (46.17%), Postives = 329/535 (61.50%), Query Frame = 2

Query:  224 PIVWTIAGSDSGGGAGIQADLHSIHALGAHGCSVITAMTAQNSHAVTHVEYASVEMIKATIAALEEDLPAAVVKLGMMGTDAVVCAVGEFLDRYKGKVVCDPVMISTSGAHLMAEGAEMRMVERIFPRATMITPNLVEAEALIGRRLRTPVEVEAGAAELLASSGAGGILIKGGHAAERLVANTAGENEVGEGRQLDMGAASDDISIQSSSSRLFSQDYWTDGTLGGTFWLTAPRVENNNTHGTGCTLSSAAAACLALGFNPTDSVVVAKAYVTQGIRVAKRLGKGGPGPVVQTAWPSRSDCFPWVTVTAEEGAGAGRPEAFSPCHSDWGVYPVVETAEWVKRLLALGVKDIQLRIKGASPEALDAEISTAAAACRASTAATGAGRLWVNDFWREAIKHGAYGVHLGQEDLEAGGGEALAAIAAAGLRLGVSTHSYLELARATAVRPSYISLGPVFETISKKVAFSPRGAGLVRTWRALVDARMPLIAIGGITLGTAPQIVEAGADSIAVISAVTKAEDVEQAVKHWNAVFATKA 1828
            PI WTIAGSDSGGGAGIQADL + H  G HGC+VITA+TAQNS AV HV       + A I AL+ DL A V+KLGM+ ++ VV  V ++L+ + G VVCDPVM+S+SG  LM  G    ++E++ PRA ++TPNL EAEA + R +    +VE  AA+++A  GA  +LI GGH + R        NE                            DYWTDG  G  FWL+ P ++  NTHGTGC+LSSA AA +A G++  D++V+AKAYV+QG+R++ +LG G PGPV    WP     FP +     E    G    F+ C  + G+YPVV++A W+ RLLA GVK IQLR K  S +AL  EI  A A  +   A     R+++ND+W+ AI  GAYGVHLGQEDL+      +AAIA AGLRLGVSTH+Y E+ARA ++RPSYI++GPV+ T SK++ + P+G   +R W  ++ A   L AIGGI    A  ++  G  S+A+ISA+T+A+D E  V    A+    A
Sbjct:    7 PIAWTIAGSDSGGGAGIQADLATFHDFGVHGCTVITAITAQNSFAVGHVSVTPRRTLAAQINALDSDLQADVIKLGMLASEDVVTMVSKYLESFPGFVVCDPVMVSSSGGELMPSGICDLLIEKLLPRADLLTPNLSEAEAFLNRSIDGLDDVERAAADIVAL-GARSVLITGGHLSAR-------NNE--------------------------RHDYWTDGEQG--FWLSGPNIDTLNTHGTGCSLSSAIAAVVARGYSLPDALVIAKAYVSQGLRMSVQLGSG-PGPVAHMGWPQHVIDFPSLNRRLPE---RGSRTKFASCDGELGLYPVVDSAAWIARLLACGVKTIQLRTKDLSGDALQQEIREAVAIGKKFNA-----RVFINDYWQLAISEGAYGVHLGQEDLDTAD---IAAIAEAGLRLGVSTHTYYEVARAHSLRPSYIAIGPVYATTSKQMPWIPQGLPQLRRWVGMLSADYTLTAIGGIDSKRAKDVLATGVGSVAMISAITQADDPEAVVAELMALHEAGA 493