mRNA_H-paniculata_contig13605.2227.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig13605.2227.1
Unique NamemRNA_H-paniculata_contig13605.2227.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5JLG6_9PHAE (Reverse transcriptase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JLG6_9PHAE)

HSP 1 Score: 133 bits (334), Expect = 9.560e-33
Identity = 72/179 (40.22%), Postives = 107/179 (59.78%), Query Frame = 1
Query:    4 KREAILEEVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAM--RELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKEKG 534
            KR AI+ + A   P L  ++A+CY E  A  IY M++GERR I   +G+ QG+G+GP LFC  +  I+   R +++ LGV L AYVDD+++  + +T E ++ I  LV EL  +G+ +   K+ A+ P  H  T  E   L   G+ +AE G  VV G PIG+DA+V+  A+ V+ E G
Sbjct:  159 KRTAIMAQAAKSVPDLVGYIARCYDEIPAKAIYTMDSGERRTIECKSGVQQGDGMGPPLFCFILVPIVSKVRAKYDHLGVSLKAYVDDISLHFKNITAENIQVIPDLVDELEAVGIIVNRGKSSALPPPGHDVTPAERRLLGDAGLPIAEEGITVV-GVPIGTDAYVEDIAMKVITEGG 336          
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5LL14_9PHAE (Reverse transcriptase domain-containing protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LL14_9PHAE)

HSP 1 Score: 130 bits (328), Expect = 4.660e-32
Identity = 72/180 (40.00%), Postives = 105/180 (58.33%), Query Frame = 1
Query:    4 KREAILEEVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDD---LAMRELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKEKG 534
            KR AI+ + A   P L  ++A+CY E  A  IY M++GERR I   +G+ QG+G+GP LFC  +  I+   R +++ LGV L AY+DD   L  + +T E ++ I  LV EL  +G+ +   K+ A+ P  H  T  E   L   G+ +AE G  VV G PIG+DA+V+  A+ V+ E G
Sbjct:  151 KRTAIMAQAAKSVPDLVGYIARCYDEIPAKAIYTMDSGERRTIECKSGVQQGDGMGPPLFCFTLVPIVLKLRAKYDNLGVRLKAYMDDIISLHFKNITAENIQVIPDLVDELEAVGIIVNRGKSSALPPPGHDVTPAERRLLGDAGLPIAEEGITVV-GVPIGTDAYVEDIAMKVITEGG 329          
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5KMQ2_9PHAE (Reverse transcriptase domain-containing protein n=16 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KMQ2_9PHAE)

HSP 1 Score: 130 bits (326), Expect = 1.180e-31
Identity = 71/179 (39.66%), Postives = 107/179 (59.78%), Query Frame = 1
Query:    4 KREAILEEVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAM--RELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKEKG 534
            KR AI+ + A   P L  ++A+CY E  A  IY M++GERR I   +G+ QG+G+GP LFC  +  I+   R +++ LGV L AY+DD+++  + +T E ++ I  LV EL  +G+ +   K+ A+ P  H  T  E   L   G+ +AE G  VV G PIG+DA+V+  A+ V+ E G
Sbjct:  520 KRTAIMAQAAKSVPDLVGYIARCYDEIPAKAIYAMDSGERRTIECKSGVQQGDGMGPPLFCFILVPIVLKLRAKYDHLGVCLKAYMDDISLHFKNITAENIQVIPDLVDELEAVGIIVNRGKSSALPPPGHDVTPAERRLLGDAGLPIAEEGITVV-GVPIGTDAYVEDIAMKVITEGG 697          
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5KYJ9_9PHAE (Reverse transcriptase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KYJ9_9PHAE)

HSP 1 Score: 129 bits (323), Expect = 2.480e-31
Identity = 70/179 (39.11%), Postives = 107/179 (59.78%), Query Frame = 1
Query:    4 KREAILEEVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAM--RELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKEKG 534
            KR AI+ + A   P L  ++A+CY E  A  IY M++GERR I   +G+ QG+G+GP LFC  +  I+   R +++ LGV L AY+DD+++  + +T E ++ I  +V EL  +G+ +   K+ A+ P  H  T  E   L   G+ +AE G  VV G PIG+DA+V+  A+ V+ E G
Sbjct:   67 KRTAIMAQAAKSVPDLVGYIARCYDEIPAKAIYTMDSGERRTIECKSGVQQGDGMGPPLFCFILVPIVLKLRAKYDHLGVCLKAYMDDISLHFKNITAENIQVIPDVVDELEAVGIIVNRGKSSALPPPGHDVTPAERRLLGDAGLPIAEEGITVV-GVPIGTDAYVEDIAMKVITEGG 244          
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5KKQ2_9PHAE (Reverse transcriptase domain-containing protein n=3 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KKQ2_9PHAE)

HSP 1 Score: 127 bits (320), Expect = 6.910e-31
Identity = 69/179 (38.55%), Postives = 107/179 (59.78%), Query Frame = 1
Query:    4 KREAILEEVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAM--RELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKEKG 534
            KR AI+ + +   P L  ++A+CY E  A  IY M++GERR I   +G+ QG+G+GP LFC  +  I+   R +++ LGV L AY+DD+++  +++T E ++    LV EL  +G+ +   K+ A+ P  H  T  E   L   G+ +AE G  VV G PIG+DA+V+  A+ V+ E G
Sbjct:  519 KRTAIMAQASKSVPDLVGYIARCYDEIPAKAIYTMDSGERRTIECKSGVQQGDGMGPPLFCFILVPIVLKLRAKYDHLGVCLKAYMDDISLHFKDITAENIQVTPDLVDELEAVGIIVNRGKSSALPPPGHDVTPTERRPLGDAGLPIAEEGITVV-GVPIGTDAYVEDIAMKVITEGG 696          
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5KJC0_9PHAE (Reverse transcriptase domain-containing protein (Fragment) n=3 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KJC0_9PHAE)

HSP 1 Score: 127 bits (319), Expect = 9.520e-31
Identity = 70/179 (39.11%), Postives = 106/179 (59.22%), Query Frame = 1
Query:    4 KREAILEEVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAM--RELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKEKG 534
            KR AI+ + A   P L  ++A+CY E  A  IY M++GERR I   +G+ QG+G+GP LFC  +  I+   R +++ LGV L AY+DD+++  + +T E ++ I  LV EL  +G+ +   K+ A+ P  H  T  E   L   G+ +AE G  VV G PI +DA+V+  A+ V+ E G
Sbjct:  546 KRTAIMAQAAKSVPDLVGYIARCYDEIPAKAIYTMDSGERRTIECKSGVQQGDGMGPPLFCFILVPIVLKLRAKYDHLGVSLKAYMDDISLHFKNITAEFIQVIPDLVNELEAVGIIVNRGKSSALPPPGHDVTPAERRLLGDAGLPIAEEGITVV-GVPIETDAYVEDIAMKVITEGG 723          
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5JI32_9PHAE (Reverse transcriptase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JI32_9PHAE)

HSP 1 Score: 125 bits (315), Expect = 3.390e-30
Identity = 69/179 (38.55%), Postives = 105/179 (58.66%), Query Frame = 1
Query:    4 KREAILEEVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAM--RELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKEKG 534
            KR AI+ + A   P L  ++A+CY E  A  IY M++GERR I   +G+ QG+G+GP LFC  +  I+   R +++ LGV L AY+DD+++  + +T E ++ I  LV EL  +G+ +   K+ A+ P        E   L   G+ +AE G  VV G PIG+DA+V+  A+ V+ E G
Sbjct:  541 KRTAIMAQAAKSVPDLVGYIARCYDEIPAKAIYTMDSGERRTIECKSGVQQGDGMGPPLFCFILVPIVLKLRAKYDHLGVCLKAYMDDISLHFKNITAENIQVIPDLVDELEAVGIIVNRGKSSALHPPGRDVAPAERRLLGDAGLPIAEEGITVV-GVPIGTDAYVEDIAIKVIAEGG 718          
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5K0V7_9PHAE (Reverse transcriptase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K0V7_9PHAE)

HSP 1 Score: 118 bits (296), Expect = 5.050e-28
Identity = 66/180 (36.67%), Postives = 101/180 (56.11%), Query Frame = 1
Query:    4 KREAILEEVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAMR--ELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKEKGE 537
            KR  I+ + A   P +  ++A+CY E  A  IY M++GERR I   +G+ QG+G+GP LFC  +  I+   R  ++ LGV L AY+DD+++    +T E ++ I  LV EL  +G+ +   K+ A+ P  H  T  E   L   G+ +AE G  VV G PIG+DA+V+       + + E
Sbjct:  144 KRTVIMAQAAKSVPDVLGYIARCYDEIPAKAIYTMDSGERRTIECKSGVQQGDGMGPPLFCFILVPIVLKLRAMYDHLGVCLKAYMDDISLHFNNITAENIQLIPDLVDELEAVGIIVNRGKSSALPPPGHDVTPAERRLLGDAGLPIAEEGITVV-GVPIGTDAYVEDKLQAAPRRRKE 322          
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5JHZ7_9PHAE (Reverse transcriptase domain-containing protein (Fragment) n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHZ7_9PHAE)

HSP 1 Score: 116 bits (291), Expect = 4.810e-27
Identity = 64/170 (37.65%), Postives = 99/170 (58.24%), Query Frame = 1
Query:   25 EVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAM--RELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKE 528
            + A   P L  ++A+CY E  A  IY M++GERR I   +G+ QG+G+GP LFC  +  I+   R +++ LGV L AY+DD+++  + +T E ++ I  LV EL  +G+ +   K+ A+ P  H  T      L   G+ +AE G  VV G PIG+D +V+  A+ V+ E
Sbjct:    3 QAAKSVPDLVGYIARCYDEIPAKAIYTMDSGERRTIECKSGVQQGDGMGPPLFCFILVPIVLKLRAKYDHLGVCLKAYMDDISLHFKNITAENIQVIPDLVDELEAVGIIVNRGKSSALPPPGHDVTPAGRRLLGDAGLPIAEEGITVV-GVPIGTDVYVEDIAMKVMTE 171          
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Match: A0A6H5J7C4_9PHAE (Reverse transcriptase domain-containing protein (Fragment) n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J7C4_9PHAE)

HSP 1 Score: 102 bits (253), Expect = 1.560e-22
Identity = 56/145 (38.62%), Postives = 87/145 (60.00%), Query Frame = 1
Query:  106 METGERRRITSNTGIHQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAM--RELTEEKLRAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAERGGAVVTGAPIGSDAFVQQYAVTVVKEKG 534
            M++GERR I   +G+ QG+G+GP LFC  +  I+   R +++ LGV L +Y+DD+++  + +T E ++ I  LV EL  +G+ +   K+ A+ P  H  T  E   L   G+ +AE G  VV G PIG DA+V+  A+ V+ E G
Sbjct:    1 MDSGERRTIECKSGVQQGDGMGPPLFCFILVPIVLKLRAKYDHLGVCLKSYMDDISLHFKNITAENIQVIPDLVDELEAVGIIVNRGKSSALPPPGHDVTPAERRLLGDAGLPIAEEGITVV-GVPIGKDAYVEDIAMKVITEGG 144          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig13605.2227.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 20
Match NameE-valueIdentityDescription
A0A6H5JLG6_9PHAE9.560e-3340.22Reverse transcriptase domain-containing protein n=... [more]
A0A6H5LL14_9PHAE4.660e-3240.00Reverse transcriptase domain-containing protein (F... [more]
A0A6H5KMQ2_9PHAE1.180e-3139.66Reverse transcriptase domain-containing protein n=... [more]
A0A6H5KYJ9_9PHAE2.480e-3139.11Reverse transcriptase domain-containing protein n=... [more]
A0A6H5KKQ2_9PHAE6.910e-3138.55Reverse transcriptase domain-containing protein n=... [more]
A0A6H5KJC0_9PHAE9.520e-3139.11Reverse transcriptase domain-containing protein (F... [more]
A0A6H5JI32_9PHAE3.390e-3038.55Reverse transcriptase domain-containing protein n=... [more]
A0A6H5K0V7_9PHAE5.050e-2836.67Reverse transcriptase domain-containing protein n=... [more]
A0A6H5JHZ7_9PHAE4.810e-2737.65Reverse transcriptase domain-containing protein (F... [more]
A0A6H5J7C4_9PHAE1.560e-2238.62Reverse transcriptase domain-containing protein (F... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig13605contigH-paniculata_contig13605:199..735 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons1
Model size537
Cds size537
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929077.5237274-CDS-H-paniculata_contig13605:198..7351622929077.5237274-CDS-H-paniculata_contig13605:198..735Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13605 199..735 -
1691480723.4667463-CDS-H-paniculata_contig13605:198..7351691480723.4667463-CDS-H-paniculata_contig13605:198..735Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13605 199..735 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig13605.2227.1prot_H-paniculata_contig13605.2227.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig13605 199..735 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig13605.2227.1

>prot_H-paniculata_contig13605.2227.1 ID=prot_H-paniculata_contig13605.2227.1|Name=mRNA_H-paniculata_contig13605.2227.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=179bp
VKREAILEEVAIRTPGLTPFVAKCYGEKSADIIYQMETGERRRITSNTGI
HQGEGLGPALFCMPVGSILRMCREQFEALGVELLAYVDDLAMRELTEEKL
RAIHFLVQELARIGVQIKNAKTKAVSPMNHTSTEREIAALAAMGISVAER
GGAVVTGAPIGSDAFVQQYAVTVVKEKGE
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mRNA from alignment at H-paniculata_contig13605:199..735-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig13605.2227.1 ID=mRNA_H-paniculata_contig13605.2227.1|Name=mRNA_H-paniculata_contig13605.2227.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=537bp|location=Sequence derived from alignment at H-paniculata_contig13605:199..735- (Halopteris paniculata Hal_grac_a_UBK monoicous)
GTCAAGCGGGAGGCGATCTTGGAAGAAGTGGCAATCCGCACCCCAGGACT GACACCCTTCGTAGCCAAATGCTACGGGGAGAAGTCGGCAGATATAATTT ACCAGATGGAAACCGGCGAACGCAGGAGAATCACCTCGAACACGGGAATC CACCAGGGCGAAGGATTGGGGCCCGCCCTCTTCTGCATGCCCGTGGGATC GATCTTGCGCATGTGCCGAGAACAATTCGAGGCTCTGGGGGTGGAGTTAT TGGCTTACGTGGACGATCTGGCAATGAGGGAGCTGACGGAAGAAAAATTA CGGGCGATCCATTTCCTCGTGCAAGAGCTAGCACGCATAGGAGTGCAGAT TAAGAACGCCAAAACGAAGGCAGTGTCTCCAATGAACCACACCTCCACCG AAAGGGAGATCGCCGCGCTGGCGGCGATGGGCATCAGCGTTGCAGAGAGA GGGGGAGCGGTGGTGACGGGTGCACCGATTGGGTCCGACGCATTCGTTCA GCAGTACGCCGTCACTGTAGTCAAGGAAAAGGGGGAA
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Coding sequence (CDS) from alignment at H-paniculata_contig13605:199..735-

>mRNA_H-paniculata_contig13605.2227.1 ID=mRNA_H-paniculata_contig13605.2227.1|Name=mRNA_H-paniculata_contig13605.2227.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=1074bp|location=Sequence derived from alignment at H-paniculata_contig13605:199..735- (Halopteris paniculata Hal_grac_a_UBK monoicous)
GTCAAGCGGGAGGCGATCTTGGAAGAAGTGGCAATCCGCACCCCAGGACT
GACACCCTTCGTAGCCAAATGCTACGGGGAGAAGTCGGCAGATATAATTT
ACCAGATGGAAACCGGCGAACGCAGGAGAATCACCTCGAACACGGGAATC
CACCAGGGCGAAGGATTGGGGCCCGCCCTCTTCTGCATGCCCGTGGGATC
GATCTTGCGCATGTGCCGAGAACAATTCGAGGCTCTGGGGGTGGAGTTAT
TGGCTTACGTGGACGATCTGGCAATGAGGGAGCTGACGGAAGAAAAATTA
CGGGCGATCCATTTCCTCGTGCAAGAGCTAGCACGCATAGGAGTGCAGAT
TAAGAACGCCAAAACGAAGGCAGTGTCTCCAATGAACCACACCTCCACCG
AAAGGGAGATCGCCGCGCTGGCGGCGATGGGCATCAGCGTTGCAGAGAGA
GGGGGAGCGGTGGTGACGGGTGCACCGATTGGGTCCGACGCATTCGTTCA
GCAGTACGCCGTCACTGTAGTCAAGGAAAAGGGGGAAGTCAAGCGGGAGG
CGATCTTGGAAGAAGTGGCAATCCGCACCCCAGGACTGACACCCTTCGTA
GCCAAATGCTACGGGGAGAAGTCGGCAGATATAATTTACCAGATGGAAAC
CGGCGAACGCAGGAGAATCACCTCGAACACGGGAATCCACCAGGGCGAAG
GATTGGGGCCCGCCCTCTTCTGCATGCCCGTGGGATCGATCTTGCGCATG
TGCCGAGAACAATTCGAGGCTCTGGGGGTGGAGTTATTGGCTTACGTGGA
CGATCTGGCAATGAGGGAGCTGACGGAAGAAAAATTACGGGCGATCCATT
TCCTCGTGCAAGAGCTAGCACGCATAGGAGTGCAGATTAAGAACGCCAAA
ACGAAGGCAGTGTCTCCAATGAACCACACCTCCACCGAAAGGGAGATCGC
CGCGCTGGCGGCGATGGGCATCAGCGTTGCAGAGAGAGGGGGAGCGGTGG
TGACGGGTGCACCGATTGGGTCCGACGCATTCGTTCAGCAGTACGCCGTC
ACTGTAGTCAAGGAAAAGGGGGAA
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