mRNA_H-paniculata_contig13042.1907.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig13042.1907.1
Unique NamemRNA_H-paniculata_contig13042.1907.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: A0A6H5JIM0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JIM0_9PHAE)

HSP 1 Score: 154 bits (388), Expect = 3.050e-42
Identity = 73/180 (40.56%), Postives = 115/180 (63.89%), Query Frame = 1
Query:    1 LAAEFGVRERYPRKVWANLKEQLDRGHTISLRSARRSGRSSDFTPTKQVRIREINANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRITPSLSFHHKKNRVDFILDQVARDRRKFKTGFNTIHVDEAWFYLIHDKEKVRMFPGEEKVGSIKVQHKSHILKVMFICAVSRP 540
            +   +GV ++YP ++W  +K Q+D    + L +  RSGR S  TP+K   ++ +N+ NR+ T++Q+S +L +LGLE G   +  W   +GAA+   RI PSLS   K++R+DFI D+V     ++    N +H+DE+WFYL+ +KEKVR+F GE+  G  +V H+SH+ K+M I A +RP
Sbjct:   24 ICERYGVGKKYPSELWKRVKTQIDNTQELDLSNEERSGRPSLLTPSKIAALKTVNSQNRSSTLRQVSDQLTDLGLEFGKDTVRRWFSKLGAAKKAGRIKPSLSDAQKRHRMDFISDRVDEPIGEYLDQGNVVHLDESWFYLMREKEKVRVFSGEDVPGPPRVPHESHLPKIMVIVANARP 203          
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: W2YNB0_PHYPR (Uncharacterized protein (Fragment) n=1 Tax=Phytophthora parasitica P10297 TaxID=1317064 RepID=W2YNB0_PHYPR)

HSP 1 Score: 75.1 bits (183), Expect = 7.940e-13
Identity = 57/177 (32.20%), Postives = 92/177 (51.98%), Query Frame = 1
Query:   40 KVWANLKEQLDRGHTISLRSAR--RSGRSSDFTPTKQVRIRE----INANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRITPSLSFHHKKNRVDFILDQVAR---DRRKFKTGFNTIHVDEAWFYLIHDKEKVRMFPGE-EKVGSIKVQHKSHILKVMFICAVSRP 540
            ++W    + +  G  I   S R  RSGR S  T   +V++ E    I   +R   ++ L+S L      +  S LH++ +A         + P L+   +  RV+F    V R   +R  F +  + +H+DE WF+L  DK+  R + GE E+V  I V++K++I+KVMF+CAV+RP
Sbjct:   57 RLWRRSVKAIQAGAPIDYESGRKGRSGRKSRLTSEFRVQLNEAIELIPLEDRA-DIRTLASSLG-----IAKSTLHDYYQAGAFRSHSAHVKPLLTDKQRAARVEFAAGFVHRGFGERLTFNSMMDFVHLDEKWFFLKKDKQ--RFYLGENEEVPHITVKNKNYIIKVMFLCAVTRP 225          
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: A0A397ATC7_9STRA (Uncharacterized protein n=6 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A397ATC7_9STRA)

HSP 1 Score: 72.8 bits (177), Expect = 2.710e-12
Identity = 60/186 (32.26%), Postives = 90/186 (48.39%), Query Frame = 1
Query:    1 LAAEFGVRERYPRKVWANLKEQLDRG----HTISLRSARRSGRSSDFTPTKQVRIREINANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRITPSLSFHHKKNRVDFILDQVAR-DRRKFKTG-FNTIHVDEAWFYLIHDKEKVRMFPGEEKVGSIKVQHKSHILKVMFICAVSRP 540
            +A  FG      R++W      L       H++  R   +SGR   +T   Q RI+ I  + R        S    LG+   +S LH + K    A+    + P L+  +K  R+++ LDQV   D  KF    ++T+HVDE WF++   + KV   PGE K+     + K H+LKVMF+ AV+RP
Sbjct:   40 VAEMFGCTPTTVRRIWKRASVDLSGNKTICHSVGQRKKGKSGRKQLYTDLPQ-RIQTIPQSRRYC----FRSVAHALGMP--TSTLHTYYKRGVIAKYSSVVKPLLTDSNKVCRLNWALDQVRDIDGEKFIDAMYDTVHVDEKWFFMTRLQRKVIGAPGE-KIKQRTCKSKQHLLKVMFLSAVARP 217          
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: A0A3L6VAL4_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A3L6VAL4_9STRA)

HSP 1 Score: 72.8 bits (177), Expect = 3.070e-12
Identity = 60/186 (32.26%), Postives = 90/186 (48.39%), Query Frame = 1
Query:    1 LAAEFGVRERYPRKVWANLKEQLDRG----HTISLRSARRSGRSSDFTPTKQVRIREINANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRITPSLSFHHKKNRVDFILDQVAR-DRRKFKTG-FNTIHVDEAWFYLIHDKEKVRMFPGEEKVGSIKVQHKSHILKVMFICAVSRP 540
            +A  FG      R++W      L       H++  R   +SGR   +T   Q RI+ I  + R        S    LG+   +S LH + K    A+    + P L+  +K  R+++ LDQV   D  KF    ++T+HVDE WF++   + KV   PGE K+     + K H+LKVMF+ AV+RP
Sbjct:   40 VAEMFGCTPTTVRRIWKRASVDLSGNKTIFHSVGQRKKGKSGRKQLYTDLPQ-RIQTIPQSRRYC----FRSVAHALGMP--TSTLHTYYKRGVIAKYSSVVKPLLTDSNKVCRLNWALDQVRDIDGEKFIDAMYDTVHVDEKWFFMTRLQRKVIGAPGE-KIKQRTCKSKQHLLKVMFLSAVARP 217          
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: A0A1R3I5M9_9ROSI (Uncharacterized protein n=1 Tax=Corchorus olitorius TaxID=93759 RepID=A0A1R3I5M9_9ROSI)

HSP 1 Score: 72.0 bits (175), Expect = 1.630e-11
Identity = 53/180 (29.44%), Postives = 89/180 (49.44%), Query Frame = 1
Query:    1 LAAEFGVRERYPRKVWANLKEQLDRGHTISLRSARRSGRSSDFTPTKQVRIREINANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRITPSLSFHHKKNRVDFILDQVARDRRKFKTGFNTIHVDEAWFYLIHDKEKVRMFPGEEKVGSIKVQHKSHILKVMFICAVSRP 540
            LA+ FGV  +  +++W   KE    G+   +++ +  GR       K++++         L+ +     +A+  L++  S LH  LK     R    + P+L+F +K  R+ F L  +  +   FK  ++TIH+DE WFYLI       + P EE+      + K+ I KVMF+ AV+RP
Sbjct:   54 LASSFGVCTKSIQRLWKRAKET---GNVYHMKT-KNCGR-------KRIQLDSDQFCEIPLSRRSTLRSMAD-ALKISKSTLHRLLKIGAILRHSSPLRPNLTFENKIARLRFCLKMLESNDSTFKGMYDTIHIDEKWFYLIRKSVNYYLLPDEEEPHRT-CKSKNFIPKVMFLAAVARP 220          
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: UPI0006A6D7E0 (uncharacterized protein LOC106302859 n=1 Tax=Brassica oleracea var. oleracea TaxID=109376 RepID=UPI0006A6D7E0)

HSP 1 Score: 70.1 bits (170), Expect = 1.690e-11
Identity = 41/112 (36.61%), Postives = 61/112 (54.46%), Query Frame = 1
Query:  217 LGLEVGSSKLHNWLKAMGAARVQRRITPSLSFHHKKNRVDFILDQVARD----RRKFKTGFNTIHVDEAWFYLIHDKEKVRMFPGEEKVGSIKVQHKSHILKVMFICAVSRP 540
            +GL + +S LH  +K     R    I P LS  + + R+ F L  + +D      KF   +N++H+DE WFY+    EK  + P EE+V     Q K+HI KVMF+ A++RP
Sbjct:    1 MGLGIATSTLHKRVKEGILRRHFNAIKPGLSDENMRARLKFCLSMLEKDSLAHEPKFVDMYNSVHIDEKWFYMTKKTEKYYVVP-EEEVPHRTCQSKNHITKVMFLAAMARP 111          
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: I6THG9_PSEMU (Tranposase n=1 Tax=Pseudo-nitzschia multiseries TaxID=37319 RepID=I6THG9_PSEMU)

HSP 1 Score: 72.0 bits (175), Expect = 1.730e-11
Identity = 59/184 (32.07%), Postives = 92/184 (50.00%), Query Frame = 1
Query:    4 AAEFGVRERYPRKVWANLKEQLD-----RGHTISLRSARRSGRSSDFTPTKQVRIREINANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRITPSLSFHHKKNRVDFILDQVARDRRKFKTGFNTIHVDEAWFYLIHDKEKVRMFPGEEKVGSIKVQHKSHILKVMFICAVSRP 540
            AA FGV  R   +VW    E +D      G   + R  +  GR S F   ++  +++++  +R+     L +  A  G  +  + L N++K     R    + P L+  +KK RV+F L  +  D  KF+  ++TIH+DE WFYL    +K  +  GE +  S   + K  I KVMF+CAV+RP
Sbjct:  108 AALFGVTARTCSRVWKMYLETVDPETCPAGDVSTNR--KNCGRKSTFAAVEEA-VKKVSYKDRS----DLRTLSAASG--IARTTLGNYVKKGQILRHSGAVKPFLTEKNKKARVEFALSLIKPDG-KFEDMYDTIHIDEKWFYLKTTNQKTYLCSGEPEPHST-CKSKRFIEKVMFLCAVARP 280          
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: A0A1R3G118_9ROSI (Uncharacterized protein n=1 Tax=Corchorus olitorius TaxID=93759 RepID=A0A1R3G118_9ROSI)

HSP 1 Score: 68.6 bits (166), Expect = 2.350e-10
Identity = 53/180 (29.44%), Postives = 88/180 (48.89%), Query Frame = 1
Query:    1 LAAEFGVRERYPRKVWANLKEQLDRGHTISLRSARRSGRSSDFTPTKQVRIREINANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRITPSLSFHHKKNRVDFILDQVARDRRKFKTGFNTIHVDEAWFYLIHDKEKVRMFPGEEKVGSIKVQHKSHILKVMFICAVSRP 540
            LA+ FGV  +  + +W   KE    G+   +++ +  GR      + Q   REI  + R+ T++ ++       L++  S LH  LK     R    + P+L+   K  R+ F L ++  +   FK  ++TIH+DE WFYL        + P EE+      + K+ I KVMF+ A++RP
Sbjct:  146 LASSFGVCTKSIQCLWKRAKET---GNVYHMKT-KNCGRKRIQLDSDQ--FREIPLSRRS-TLRSMAD-----ALKISKSTLHRLLKIGAILRHSSPLRPNLTLEKKITRLRFCLKKLESNDSTFKDMYDTIHIDEKWFYLTKKSVNYYLLPDEEEPHR-NCKSKNFIPKVMFLAALARP 312          
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: UPI00053F8527 (uncharacterized protein LOC104897330 n=1 Tax=Beta vulgaris subsp. vulgaris TaxID=3555 RepID=UPI00053F8527)

HSP 1 Score: 68.6 bits (166), Expect = 2.770e-10
Identity = 57/187 (30.48%), Postives = 88/187 (47.06%), Query Frame = 1
Query:    1 LAAEFGVRERYPRKVWANLKEQLDRGHTISLRS--ARRSGRSSDFTPTKQVRIREINANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRIT-PSLSFHHKKNRVDFILDQVARDRR----KFKTGFNTIHVDEAWFYLIHDKEKVRMFPGEEKVGSIKVQHKSHILKVMFICAVSRP 540
            +A + G+      ++W + K+Q + GH I +R+  A R GR       K   ++ +  N RT      S +     L++  S +++ LK  G  R     T P L+  HK  R+ ++L  +    R    KF    N +H+DE WFYL  D  +  + P E+   S   Q K   +KVMF+CA SRP
Sbjct:  116 IAKKHGISRMTLDRIWKDAKKQREAGHAIDVRNKKAGRCGRKLKVYDDKW--LQSVPLNLRT------SIRAFAGYLKIPPSTVYSLLKR-GKLRSHTSSTHPKLTEDHKIQRMKWVLQHILPATRTQPPKFVDMQNVVHIDEKWFYLSPDMRRFYLLPAEDDPYSC-CQSKRFKIKVMFMCATSRP 292          
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Match: A0A1R3HLY9_COCAP (Urease domain-containing protein n=1 Tax=Corchorus capsularis TaxID=210143 RepID=A0A1R3HLY9_COCAP)

HSP 1 Score: 68.6 bits (166), Expect = 2.790e-10
Identity = 54/180 (30.00%), Postives = 89/180 (49.44%), Query Frame = 1
Query:    1 LAAEFGVRERYPRKVWANLKEQLDRGHTISLRSARRSGRSSDFTPTKQVRIREINANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRITPSLSFHHKKNRVDFILDQVARDRRKFKTGFNTIHVDEAWFYLIHDKEKVRMFPGEEKVGSIKVQHKSHILKVMFICAVSRP 540
            LAA FGV  +  +++W   KE    G+   +++ +  GR      + Q   R+I  + R+ T++ ++       L++  S LH  LK     R    + P+L+  +K  R+ F L  +  D   FK  ++TIH+DE WFYL        +   EE+   +  + K+ I KVMF+ AV+RP
Sbjct:  196 LAASFGVCTKTIQRLWKRAKET---GNVFHMKT-KNCGRKRIQLDSDQ--FRQIPLSRRS-TLRSMAD-----ALKISKSTLHRLLKIGAILRHSSSLRPNLTPENKVVRLRFCLKMLESDDSTFKGMYDTIHIDEKWFYLTRKSVNYYLLLDEEEPHRM-CKSKNFIPKVMFLTAVARP 362          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig13042.1907.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JIM0_9PHAE3.050e-4240.56Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
W2YNB0_PHYPR7.940e-1332.20Uncharacterized protein (Fragment) n=1 Tax=Phytoph... [more]
A0A397ATC7_9STRA2.710e-1232.26Uncharacterized protein n=6 Tax=Aphanomyces astaci... [more]
A0A3L6VAL4_9STRA3.070e-1232.26Uncharacterized protein n=1 Tax=Aphanomyces astaci... [more]
A0A1R3I5M9_9ROSI1.630e-1129.44Uncharacterized protein n=1 Tax=Corchorus olitoriu... [more]
UPI0006A6D7E01.690e-1136.61uncharacterized protein LOC106302859 n=1 Tax=Brass... [more]
I6THG9_PSEMU1.730e-1132.07Tranposase n=1 Tax=Pseudo-nitzschia multiseries Ta... [more]
A0A1R3G118_9ROSI2.350e-1029.44Uncharacterized protein n=1 Tax=Corchorus olitoriu... [more]
UPI00053F85272.770e-1030.48uncharacterized protein LOC104897330 n=1 Tax=Beta ... [more]
A0A1R3HLY9_COCAP2.790e-1030.00Urease domain-containing protein n=1 Tax=Corchorus... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig13042contigH-paniculata_contig13042:2878..3417 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score81.3
Seed ortholog evalue5.2e-13
Seed eggNOG ortholog38727.Pavir.J34752.1.p
Hectar predicted targeting categoryother localisation
EggNOG OGs2CMKC@1,2QQNW@2759,37TMG@33090,3GG9M@35493,3IN47@38820,3M58A@4447
Best tax levelPoales
Best eggNOG OGNA|NA|NA
Exons1
Model size540
Cds size540
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929030.4017785-CDS-H-paniculata_contig13042:2877..34171622929030.4017785-CDS-H-paniculata_contig13042:2877..3417Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13042 2878..3417 +
1691480705.8767042-CDS-H-paniculata_contig13042:2877..34171691480705.8767042-CDS-H-paniculata_contig13042:2877..3417Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig13042 2878..3417 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig13042.1907.1prot_H-paniculata_contig13042.1907.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig13042 2878..3417 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig13042.1907.1

>prot_H-paniculata_contig13042.1907.1 ID=prot_H-paniculata_contig13042.1907.1|Name=mRNA_H-paniculata_contig13042.1907.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=180bp
LAAEFGVRERYPRKVWANLKEQLDRGHTISLRSARRSGRSSDFTPTKQVR
IREINANNRTLTVQQLSSKLAELGLEVGSSKLHNWLKAMGAARVQRRITP
SLSFHHKKNRVDFILDQVARDRRKFKTGFNTIHVDEAWFYLIHDKEKVRM
FPGEEKVGSIKVQHKSHILKVMFICAVSRP
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mRNA from alignment at H-paniculata_contig13042:2878..3417+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig13042.1907.1 ID=mRNA_H-paniculata_contig13042.1907.1|Name=mRNA_H-paniculata_contig13042.1907.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=540bp|location=Sequence derived from alignment at H-paniculata_contig13042:2878..3417+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
CTTGCTGCAGAGTTTGGAGTGAGAGAGAGGTATCCTCGGAAAGTATGGGC AAACTTGAAGGAACAACTGGACAGGGGGCACACCATCTCTCTGAGATCAG CACGACGATCTGGACGGTCGTCGGACTTCACACCCACGAAACAGGTCAGG ATTCGGGAAATTAACGCGAACAATCGCACGTTGACTGTCCAACAACTTTC CTCCAAGCTAGCCGAACTGGGCCTCGAAGTCGGTAGCTCGAAGCTTCACA ACTGGCTGAAGGCTATGGGAGCAGCGAGGGTTCAGCGGCGCATCACCCCA AGCCTTTCGTTCCACCACAAGAAGAACCGGGTGGACTTCATCCTGGACCA GGTCGCCCGCGATCGCAGAAAGTTCAAGACTGGCTTCAACACGATCCACG TCGACGAAGCGTGGTTCTACCTGATCCACGACAAAGAGAAGGTGCGGATG TTCCCGGGAGAGGAGAAGGTAGGCTCGATAAAGGTGCAGCACAAGAGCCA CATTCTTAAGGTAATGTTCATCTGCGCCGTGTCGCGCCCG
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Coding sequence (CDS) from alignment at H-paniculata_contig13042:2878..3417+

>mRNA_H-paniculata_contig13042.1907.1 ID=mRNA_H-paniculata_contig13042.1907.1|Name=mRNA_H-paniculata_contig13042.1907.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=1080bp|location=Sequence derived from alignment at H-paniculata_contig13042:2878..3417+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
CTTGCTGCAGAGTTTGGAGTGAGAGAGAGGTATCCTCGGAAAGTATGGGC
AAACTTGAAGGAACAACTGGACAGGGGGCACACCATCTCTCTGAGATCAG
CACGACGATCTGGACGGTCGTCGGACTTCACACCCACGAAACAGGTCAGG
ATTCGGGAAATTAACGCGAACAATCGCACGTTGACTGTCCAACAACTTTC
CTCCAAGCTAGCCGAACTGGGCCTCGAAGTCGGTAGCTCGAAGCTTCACA
ACTGGCTGAAGGCTATGGGAGCAGCGAGGGTTCAGCGGCGCATCACCCCA
AGCCTTTCGTTCCACCACAAGAAGAACCGGGTGGACTTCATCCTGGACCA
GGTCGCCCGCGATCGCAGAAAGTTCAAGACTGGCTTCAACACGATCCACG
TCGACGAAGCGTGGTTCTACCTGATCCACGACAAAGAGAAGGTGCGGATG
TTCCCGGGAGAGGAGAAGGTAGGCTCGATAAAGGTGCAGCACAAGAGCCA
CATTCTTAAGGTAATGTTCATCTGCGCCGTGTCGCGCCCGCTTGCTGCAG
AGTTTGGAGTGAGAGAGAGGTATCCTCGGAAAGTATGGGCAAACTTGAAG
GAACAACTGGACAGGGGGCACACCATCTCTCTGAGATCAGCACGACGATC
TGGACGGTCGTCGGACTTCACACCCACGAAACAGGTCAGGATTCGGGAAA
TTAACGCGAACAATCGCACGTTGACTGTCCAACAACTTTCCTCCAAGCTA
GCCGAACTGGGCCTCGAAGTCGGTAGCTCGAAGCTTCACAACTGGCTGAA
GGCTATGGGAGCAGCGAGGGTTCAGCGGCGCATCACCCCAAGCCTTTCGT
TCCACCACAAGAAGAACCGGGTGGACTTCATCCTGGACCAGGTCGCCCGC
GATCGCAGAAAGTTCAAGACTGGCTTCAACACGATCCACGTCGACGAAGC
GTGGTTCTACCTGATCCACGACAAAGAGAAGGTGCGGATGTTCCCGGGAG
AGGAGAAGGTAGGCTCGATAAAGGTGCAGCACAAGAGCCACATTCTTAAG
GTAATGTTCATCTGCGCCGTGTCGCGCCCG
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