mRNA_H-paniculata_contig10889.573.1 (mRNA) Halopteris paniculata Hal_grac_a_UBK monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-paniculata_contig10889.573.1
Unique NamemRNA_H-paniculata_contig10889.573.1
TypemRNA
OrganismHalopteris paniculata Hal_grac_a_UBK monoicous (Halopteris paniculata Hal_grac_a_UBK monoicous)
Homology
BLAST of mRNA_H-paniculata_contig10889.573.1 vs. uniprot
Match: A0A6H5K6C3_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K6C3_9PHAE)

HSP 1 Score: 203 bits (517), Expect = 1.030e-56
Identity = 106/180 (58.89%), Postives = 121/180 (67.22%), Query Frame = 1
Query:   49 FLAEGGTDAELVGIFRTSMAPADHNGGPGQGRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXARCKVQAIFNEVGVVLKNAQLANINKMRSTLRDLERWEALYRHRPKHSIKDGPKAWWIFLVKCVSRPGKAAQRAKLGWLDVVRLLRLRKMYVQLYMARARHQATPEEYQQFTRRD 588
            FLA G TD +L  +FRTS+A                                             ++ K+QA FN+VG+VL N+Q+ANINKMRSTLRDLERWEALYRHRPKH IKDGPKAWW++LVKCVSRPGKA +RAKLGW DVVRLL LRK+YV+LY ARARHQATPEEYQQFTR D
Sbjct:  320 FLASGLTDEDLESMFRTSLAEGGDR----------HSYVVIPASPVFRLRVQRDGGGDSGSMGGSSKYKIQAFFNDVGLVLNNSQIANINKMRSTLRDLERWEALYRHRPKHKIKDGPKAWWVYLVKCVSRPGKAEKRAKLGWPDVVRLLSLRKLYVRLYTARARHQATPEEYQQFTRLD 489          
BLAST of mRNA_H-paniculata_contig10889.573.1 vs. uniprot
Match: D7FMF5_ECTSI (FYVE-type domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FMF5_ECTSI)

HSP 1 Score: 201 bits (511), Expect = 2.090e-55
Identity = 106/180 (58.89%), Postives = 119/180 (66.11%), Query Frame = 1
Query:   49 FLAEGGTDAELVGIFRTSMAPADHNGGPGQGRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXARCKVQAIFNEVGVVLKNAQLANINKMRSTLRDLERWEALYRHRPKHSIKDGPKAWWIFLVKCVSRPGKAAQRAKLGWLDVVRLLRLRKMYVQLYMARARHQATPEEYQQFTRRD 588
            FLA G T  +L  +FRTS+A          G                                  ++ K+QA FNEVG+VL N+Q+ANINKMRSTLRDLERWEALYRHRPKH IKDGPKAWW++LVKCVSRPGKA +RAKLGW DV RLL LRK+YV LY ARARHQATPEEYQQFTR D
Sbjct:  351 FLASGLTGEDLESMFRTSLA----------GGEDRHSYVVIPASPDFRLRVQREGGGDGGGVGGSSKYKIQAFFNEVGLVLNNSQIANINKMRSTLRDLERWEALYRHRPKHKIKDGPKAWWVYLVKCVSRPGKAEKRAKLGWPDVARLLSLRKLYVHLYTARARHQATPEEYQQFTRLD 520          
BLAST of mRNA_H-paniculata_contig10889.573.1 vs. uniprot
Match: A0A836C849_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C849_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 2.450e-8
Identity = 44/127 (34.65%), Postives = 66/127 (51.97%), Query Frame = 1
Query:  244 ARCKVQAIFNEVGVVLKNAQLANINKMRSTLRDLERWEALYRH----------------RPKHSIKD-GPKAWWIFLVKCVSRPGKAAQRAKLGWLDVVRLLRLRKMYVQLYMARARHQATPEEYQQ 573
            A+ +V    + +G+V+  AQL ++ +MR+TL + ERW A+Y+H                   H+  D  P+AWW             A+   L    V RLL  RK YV+LYMARA+H+ TP++YQ+
Sbjct:  378 AKLEVALRCSGMGIVVSRAQLEDLCQMRATLSEAERWGAVYQHVXXXXXXXXXXXXXXXXVPHARPDRDPRAWWR---------DSCARWRYLAKTFVARLLAQRKRYVELYMARAQHRETPDDYQE 495          
The following BLAST results are available for this feature:
BLAST of mRNA_H-paniculata_contig10889.573.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
A0A6H5K6C3_9PHAE1.030e-5658.89Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FMF5_ECTSI2.090e-5558.89FYVE-type domain-containing protein n=1 Tax=Ectoca... [more]
A0A836C849_9STRA2.450e-834.65Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-paniculata_contig10889contigH-paniculata_contig10889:777..5194 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Halopteris paniculata Hal_grac_a_UBK monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score201.4
Seed ortholog evalue4.7e-49
Seed eggNOG ortholog2880.D7FMF5
Hectar predicted targeting categoryother localisation
EggNOG free text desc.regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization
EggNOG OGsCOG5043@1,KOG1809@2759
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons6
Model size740
Cds size738
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-paniculata_contig10889.573.1prot_H-paniculata_contig10889.573.1Halopteris paniculata Hal_grac_a_UBK monoicouspolypeptideH-paniculata_contig10889 777..5193 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928792.8736422-CDS-H-paniculata_contig10889:776..10521622928792.8736422-CDS-H-paniculata_contig10889:776..1052Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 777..1052 +
1691480622.2733154-CDS-H-paniculata_contig10889:776..10521691480622.2733154-CDS-H-paniculata_contig10889:776..1052Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 777..1052 +
1622928792.8892035-CDS-H-paniculata_contig10889:1596..16461622928792.8892035-CDS-H-paniculata_contig10889:1596..1646Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 1597..1646 +
1691480622.2833848-CDS-H-paniculata_contig10889:1596..16461691480622.2833848-CDS-H-paniculata_contig10889:1596..1646Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 1597..1646 +
1622928792.905223-CDS-H-paniculata_contig10889:2701..28151622928792.905223-CDS-H-paniculata_contig10889:2701..2815Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 2702..2815 +
1691480622.2919474-CDS-H-paniculata_contig10889:2701..28151691480622.2919474-CDS-H-paniculata_contig10889:2701..2815Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 2702..2815 +
1622928792.9199848-CDS-H-paniculata_contig10889:4400..45091622928792.9199848-CDS-H-paniculata_contig10889:4400..4509Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 4401..4509 +
1691480622.3001604-CDS-H-paniculata_contig10889:4400..45091691480622.3001604-CDS-H-paniculata_contig10889:4400..4509Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 4401..4509 +
1622928792.9432533-CDS-H-paniculata_contig10889:4981..50141622928792.9432533-CDS-H-paniculata_contig10889:4981..5014Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 4982..5014 +
1691480622.3094518-CDS-H-paniculata_contig10889:4981..50141691480622.3094518-CDS-H-paniculata_contig10889:4981..5014Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 4982..5014 +
1622928792.9616294-CDS-H-paniculata_contig10889:5037..51931622928792.9616294-CDS-H-paniculata_contig10889:5037..5193Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 5038..5193 +
1691480622.3190162-CDS-H-paniculata_contig10889:5037..51931691480622.3190162-CDS-H-paniculata_contig10889:5037..5193Halopteris paniculata Hal_grac_a_UBK monoicousCDSH-paniculata_contig10889 5038..5193 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-paniculata_contig10889.573.1

>prot_H-paniculata_contig10889.573.1 ID=prot_H-paniculata_contig10889.573.1|Name=mRNA_H-paniculata_contig10889.573.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=polypeptide|length=246bp
CDDVQQTDQSPSLPSCFLAEGGTDAELVGIFRTSMAPADHNGGPGQGRGA
LGKGQHEYVVMPVSPSFRLRVQKGDGDGGGGARCKVQAIFNEVGVVLKNA
QLANINKMRSTLRDLERWEALYRHRPKHSIKDGPKAWWIFLVKCVSRPGK
AAQRAKLGWLDVVRLLRLRKMYVQLYMARARHQATPEEYQQFTRRDRGVS
TEGHRRARRGGGATVARAPESGITSRSTPTTSRATNLGAVATASRP
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mRNA from alignment at H-paniculata_contig10889:777..5194+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-paniculata_contig10889.573.1 ID=mRNA_H-paniculata_contig10889.573.1|Name=mRNA_H-paniculata_contig10889.573.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=mRNA|length=4418bp|location=Sequence derived from alignment at H-paniculata_contig10889:777..5194+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
TGCGACGACGTCCAGCAGACCGACCAGTCGCCGTCGCTGCCGTCATGCTT TCTCGCCGAGGGAGGCACGGATGCCGAACTGGTCGGCATATTCCGCACCT CGATGGCACCCGCGGATCACAATGGCGGACCGGGTCAGGGCCGGGGGGCG CTTGGCAAAGGGCAGCATGAGTACGTCGTCATGCCAGTGTCGCCATCGTT CCGTCTCAGGGTACAGAAGGGGGATGGAGATGGAGGTGGTGGCGCGCGGT GCAAGGTCCAGGCTATTTTCAACGAGGTAGGGGGGGATCGCGGGTCGGAG AGATAGATCTTGGTGTGTTTTGATATTAACAAAAAAAGGGAGAAACTTAA GCGTTTGTGCTGTTGTTCGTGGTTCTGTTGAGGAATTGGTTGTGTCTTGC TCCTTTGACGTGGTGCCGTGGTGCTGTGCTCAATTGGACGTATTGTCATG ATCCATTTGAGACATATCGACATCCCGCATTGAAGATGTACCGCCATGCT CAACTGAAGATGTACTATCATGCTCAACTAAAGATGTATTGCCATGCTCC ATTAAAGAGATGTTGCAATACTCCGTCGAAGAGGTATTGGCATACTCCAA TGGAGACGTATTACCATGGTCTATCGAAGAAACATCAAATTGAGAACGTA CTGTACTTCCCCGGCTTCCATTGAAGAGCATTGTCTACCTTGCATTGAGT TTAGTTATCTATTTTTTTACACTGATCATCCATGACACTGCGGAGGTTAT ACTTTTAGAAATGCCCCCGTATTGCCCCGCTGTCCCGCCGTCGCCGTTAT TCGTTCCATTACGTGCGTAGGTCGGAGTGGTTTTGAAGAACGCGCAACTA GCAAACATCAACAAGATGAGGTATGGTGGGATGACTGGATGCGCGCAGGG GACGCATTGGTTGCTGCAGAAAACGTCAGAATAGTCGTAAAACAAGTTGC TGAATTACTTATCCACGAAGTGAACAAACTTGAGGGGGGAGGGGGGGAGA TATCTACCTGAAGCACGTGTAAAGTAGGAGAAATTATGCATCGAATGTTT CGAACTAGCCTAACGAAAAGGATTCTTAACCCTTTCACGCTCCAGGTGAA GTTTCAGAACGAAAGTGGTATATGATGATTACTGTCGAGCTCTGCATGTA GTCATTGTGGGCAAATCATGCCAAGTTCCTCTTATGAATGGACTGTCCAT CTGAACAGCAGCGGCTGGGCTCTCTCTATCTCCGAAATCATGTCATACCC TATCGTTTTCCCCCGTCTTTTAAGAACACCCTTCTACTGTAGTGTGTCGT TTTCTCTGTGTTCGAAGGGATGAAGAGGGAAAAATTGTTCACGGATTGCT GTGTTTGCGAAAAACAATCAGTTTCTGGTTCCACTTTGCTCTTGCACCAG TTGGCAGTCATTGGTTAGTTGATTTGAATTATTGTCTGTGTATGGGTTGT GAACGAGCCTCATAGTTTTCTCCAACTTCGGCCATCTGCGCAAGGTTTAT ATCTACAAAACAATATTCGGGTAAATGTGCTCAGGATTCCAAAATTTGAA TCGAATGTTGGATTGTGGCTAGACTTTGTGCAGATGGCTGAGGGAAGCTG TGAAGCTCGTTCACAGTCCATGAGTGCACAGACGATAATTCAAATGGACC AACTAGTGACTACCTACTAGTGCAGGAGCAAAGCGAAATTCAAAAGCCCA TTTTGGTTCTCGCAAACATTCTAGATTAACGTCCACCCACTTCGAAGGGT CTTCCATCCCGCAAACTGTTTGCGATATTCCCCTATTTGATTGTAAAGTT TAGTAGTGCAGGGATGATCTCTGGTGAAGGTCATAGCTCCTGTCGTGCCT AGTCGAAGAGGTGCGGAAAGTACTCTACGAGATCTCACTCCCCCCGTTCC CACTCCTGGTTGGATGGCTGTTTAGATCTACCCTTCGGGACCTGGAGAGG TGGGAGGCGCTGTACAGGCATCGTCCCAAGCACAGCATTAAGGACGGTCC GAAGGCTTGGTGGATCTTCCTTGTCAAATGTGTCTCAAGGTGAGGAGAAT TGACAGCCGATTTTTCTGGTTATGGTCATTTTTTTATTGTGTCCACGGTT CGGGCCAATTAATATCAATACAACGCGTACGAGTCGTGGCTGGCACCGTT CAGCCGCGTGGTTCGAAATATTTTAATTATTTCACTTTCCGGACGAGTGC TGGTAATTCGATTGGATTTGGCGATAATTTTTAACAGTATCCATCTGAAG CTTCGGTATGAAGACGTTTATTCTACTCCCTCTGTATCACGAGTATGTGT GCTTATGGTTGAATTGTCTTACCCTTTTCAAATTTGGGCTTCATTAAAAG CAGAGGATATAATTCAAAGGTCCACGACCTCGGTTTGCACATGAACATCG ACAAAGCATAAATTGACTTGCGAAATGGTTGAGAAACCGCACTCTCGACT TCCTGTTTTTTATTCTGGAAATGCGTTTGCTTGCCGCAAAAGCTACATAG GCCAAAACAGCGGGGAACGATGGTTGATCGACGATAGCGTCTCAACAAGA CCTCCAATGAAGAAGCAGATTTTGCTGCGTTAGATTCGTTGACAAAACTT GGACACAATTCAATAATTATGTCTTTCTCATCAATCCCAAATTTTGTCGT CCGCGCCACGGCATTGAGGCGGGGCACACGAGATACAGCGAAGGTTCAGG TTTTGGTTGCTCGTGACAAACTAATTAACACGTGTTTCATGCGACCCTCA AGTGATGATGTGACGTGAGGAGGTGATACGTAGCTCTGTAGTATTTATTG ACTTTAGCCTAACTTTAGTCAAGGAATCTAACGCAGCAATTTGCTTCTTC ATCGGAGGTCTCGTTGAGACGTTATCGTCGATCAACGATCGCTTCCCGTT GTCTTGGCTGATGTAGCTTTTGTTGCAAGCAAACGCATTTCGGGAAAAAA ACCCCGGGAAGTCGAGAGTGCGGTTTCTCAATCATTTCGCAAGTCAATTT ATGCTTGGTAAATGTTCACGTGCAAAGCGAGGTCGTGGACTTCCGAATTA TATCCTCTGCTTTTAATGAAGCCAAAATTAGGAAAAGGTGTGCCTTTTTA CCATGCAAGAATGCGGCGAATATTGATGAAACGAATGAATAGGAGTAGAT GCTTAATTGTATGTGCCTACACGTGATGAAACTGGGACCAACGCTTGTAA AATGTTGTTTAGACCATGCGAAGTATTAGTTTTCGCTAAGGGGGGGTGAA AACATGTGCTAGCGATCCATAGGCGCACCGATCAGAGGTAGAAAACCGTG CACGGACCGAAAAGGGTGTACCTGTATTGATTACGGGATCGGTCCATTCC GTAAAATGTGCGGAAAATGATGGTGAGGGGCCGCTGAAGTCGTGAAACGA GAATCTCCGTTGTGATAAGTAATGAAGTTTTAATGTCTCTGGCTCAGTGT AAAATCAAGGGAGCATAGATACCTTCCCGTAACCCCTGAAATTTAGTTTC GATCAAGAATCAAGGTCACTCATTGCTTTGGTGCAAGAATGGCGTTAGGT TTCGCACCAGACACAGTGCACATAAATTTGTGTGTATATATGTTCATTGG TGCATGCATACCGCTTTCTTTCAGGCCGGGTAAAGCTGCACAACGCGCGA AGCTAGGTTGGCTTGACGTTGTGCGACTGCTGAGGTTGCGGAAGATGTAC GTACAGCTTTACATGGCTAGGGCCAGGCATCAGGTACAGTCTCCACCTTC GATACTGTACTGCTCTTCGCAATTTGCTCGCCGTCCCCTTTTTATGTACA AATGGTCCGTAGGTACCCTTTGCTGGGGTCACGATATGGACGAAACGTTG TATACGAGCAAGATGAAGACTATGAAAATGAAGTAGCTATTGTTTGGTGT TTCGTTGAAGGCGGACGACAAACAAACCATGTCTTTTCGTATATATGTAG GGCTCTATGTATATATAGAGATACTATTTTTTCTCTCCTAATTTTGCTCT TTGCTTCATGTGAGTAAGTTCTCTTAGTCAGTGATGCAATTATTGCGAGA GTGCATCCTGTGCATCGGCAGCAGCACCTTCAGAATAAACAAACTTTACA GACTAAATAGACTAAAAAAACGTACCGGGCAGAATCCGACAGTGAAGTAG TGGGGTGTTTTGAGTAACAGCACATACTGACACACCCAGGAATATGCGTA TACAGGCTACGCCGGAGGAATATCAGCAGTTCACTCGCCTCGACGAGCAG CTGACGGCTAACGAGATCGTGGCGTTTCGACTGAAGGCCATCGCCGAGCT CGAAGAGGAGGAGGCGCTACAGTTGCAAGAGCTCCAGAGTCTGGCATCAC TTCCAGATCCACACCAACAACATCGCGGGCGACGAACTTGGGGGCAGTGG CTACTGCGTCGAGACCCC
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Coding sequence (CDS) from alignment at H-paniculata_contig10889:777..5194+

>mRNA_H-paniculata_contig10889.573.1 ID=mRNA_H-paniculata_contig10889.573.1|Name=mRNA_H-paniculata_contig10889.573.1|organism=Halopteris paniculata Hal_grac_a_UBK monoicous|type=CDS|length=1476bp|location=Sequence derived from alignment at H-paniculata_contig10889:777..5194+ (Halopteris paniculata Hal_grac_a_UBK monoicous)
TGCGACGACGTCCAGCAGACCGACCAGTCGCCGTCGCTGCCGTCATGCTT
TCTCGCCGAGGGAGGCACGGATGCCGAACTGGTCGGCATATTCCGCACCT
CGATGGCACCCGCGGATCACAATGGCGGACCGGGTCAGGGCCGGGGGGCG
CTTGGCAAAGGGCAGCATGAGTACGTCGTCATGCCAGTGTCGCCATCGTT
CCGTCTCAGGGTACAGAAGGGGGATGGAGATGGAGGTGGTGGCGCGCGGT
GCAAGGTCCAGGCTATTTTCAACGAGTGCGACGACGTCCAGCAGACCGAC
CAGTCGCCGTCGCTGCCGTCATGCTTTCTCGCCGAGGGAGGCACGGATGC
CGAACTGGTCGGCATATTCCGCACCTCGATGGCACCCGCGGATCACAATG
GCGGACCGGGTCAGGGCCGGGGGGCGCTTGGCAAAGGGCAGCATGAGTAC
GTCGTCATGCCAGTGTCGCCATCGTTCCGTCTCAGGGTACAGAAGGGGGA
TGGAGATGGAGGTGGTGGCGCGCGGTGCAAGGTCCAGGCTATTTTCAACG
AGGTCGGAGTGGTTTTGAAGAACGCGCAACTAGCAAACATCAACAAGATG
AGGTCGGAGTGGTTTTGAAGAACGCGCAACTAGCAAACATCAACAAGATG
AGATCTACCCTTCGGGACCTGGAGAGGTGGGAGGCGCTGTACAGGCATCG
TCCCAAGCACAGCATTAAGGACGGTCCGAAGGCTTGGTGGATCTTCCTTG
TCAAATGTGTCTCAAGATCTACCCTTCGGGACCTGGAGAGGTGGGAGGCG
CTGTACAGGCATCGTCCCAAGCACAGCATTAAGGACGGTCCGAAGGCTTG
GTGGATCTTCCTTGTCAAATGTGTCTCAAGGCCGGGTAAAGCTGCACAAC
GCGCGAAGCTAGGTTGGCTTGACGTTGTGCGACTGCTGAGGTTGCGGAAG
ATGTACGTACAGCTTTACATGGCTAGGGCCAGGCATCAGGCCGGGTAAAG
CTGCACAACGCGCGAAGCTAGGTTGGCTTGACGTTGTGCGACTGCTGAGG
TTGCGGAAGATGTACGTACAGCTTTACATGGCTAGGGCCAGGCATCAGGC
TACGCCGGAGGAATATCAGCAGTTCACTCGCGCTACGCCGGAGGAATATC
AGCAGTTCACTCGCCGAGATCGTGGCGTTTCGACTGAAGGCCATCGCCGA
GCTCGAAGAGGAGGAGGCGCTACAGTTGCAAGAGCTCCAGAGTCTGGCAT
CACTTCCAGATCCACACCAACAACATCGCGGGCGACGAACTTGGGGGCAG
TGGCTACTGCGTCGAGACCCCGAGATCGTGGCGTTTCGACTGAAGGCCAT
CGCCGAGCTCGAAGAGGAGGAGGCGCTACAGTTGCAAGAGCTCCAGAGTC
TGGCATCACTTCCAGATCCACACCAACAACATCGCGGGCGACGAACTTGG
GGGCAGTGGCTACTGCGTCGAGACCC
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