Gvermi6751.t1 (polypeptide) Gracilaria vermiculophylla HapMaleFtJ_2017 male

BLAST of Gvermi6751.t1 vs. uniprot
Match: A0A2V3J3X5_9FLOR (Pullulanase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3J3X5_9FLOR)

HSP 1 Score: 1941 bits (5027), Expect = 0.000e+0
Identity = 946/1506 (62.82%), Postives = 1149/1506 (76.29%), Query Frame = 0

Query:    1 MPPLSSPAAYLVVHYHRPHHDYHRWLLSVGSRSYAQCVPDAFTAYGALFRLPLPPSSQLLLQPTHPALVDSTPRKLDLSSLRSVHHEKHVFLLSDHPHAFASPLPSSQLASHPFLFVTADFGPE-WLIVYGSATKRDVFPATSHYRVPAHPSLFVLDTSRLPNTISFALQKSYHHPAPSHSQPSFPTFDAPFPEHISLSSRPLKPSI---DPLSLLLQQASRSTYVSPSIPSTTLVSSPKPSPIIRPSK--QIILFYFRLDRSTPIVSIMLTASTTLEPVSLPSPYHSARAFSIDVTKLLQLHNSKTNSPQFSIVPIRSSAASTVQEPPLVWTPSLGFRVIYAQSYPYLLTPSRVDITVVYHRFDTHWADWELHVWTNATEQYPAFTTKLTPRRPSVPGQIVFHLIGHIFPYKTRVFVEPVRMHLHPAKESVDEFGHTVVAEPFRDACRRDVTREWTSAEPPAQILHFVQGDSRIYVQPPDPHQLIAKRWFRIRYRRFSPSDYNGWDLWTWDPTDPSSHRVALAPVAETSGWADFIVDRACYGSAKDICLLPRRGGSSWLEKDGVERVWNASLFGNDNEVRVKSITSDFVSPGDTDYDDLPTFTITQGSSLVLRSLDDVMSMMEAFADSEHSIVVRTPLPWGWICPPKKAGVASHPDVHFKLCKHPIHMSSNEGNQSFDHMAGRTLCHQKSKQLSPTSIRFFFDDKVAHFDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGDAGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEGENKRLFPGVEVIDPYSRCNTAHTGRGLIYGTDMTPIYDRPKLRVADNIVYELHIRDVTIDKFSGIRNGGKFLGLTERGTKMMLKSGEHPHQTPLTPWEQEIMPGADKDFSILNKFSTGLDHIAQMGVTAIQIMPIQDFDNNEADESEYRWGYMPVHFNSPDGWYATSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIRMRTDTIKFSEEDIKRMHKLAAVLVLTSQGIPLIQAGQEMCRTKFDDENSYESPDEINMIRWDAKLKEWPIVQYYRGLILLRRAHPEIFCMENAEEIHERIVFYEDCGLHVPDRCIAYRIKGNPEQLLTQLRKQNSRSGCEEEDLKEQSLQWSEVVVLFNATPVQVVFELPGKKADVMWMQTVSASVAGTRTISGPFVGAIEVSGRSAAVLRRASALECCSSQLELRLASIADVHCSFHGDDPLSRYSVGLEPRPTAEEQAIRNQSISQRQRFKKR 1500
            M P      +LVVHYHRP+H YH W L++ + +YAQ V +A T+YGALFR+P P  + + L PTHP LVD+ PR+ DL+SL +VH E HVFLL  HP A  SP    Q   +P LF  +   P  WLIVY   TKRDVFPA++HYR   HP+LF+LDTS L   I   +Q  YH PAP+ +Q S+P F AP+P+H+ LS+ PLK      D  + +  QA +  ++S  +   T++ SP    +IR  K   ++LFY+RLDRSTPIVS+ ++AS  LE V LPS YH+++AF+ID++ L + H+S +   Q SI P+RS++   + E  L W PSLG RVIYAQS+P+LLTP++ D+T+VYHR+D+ W+DWELHVW+   + YPA + KLTP RP  PGQ+VFHL+G++FPY+ +VFVEPVRM LHP   + DEFG TVV EP+RDACRRDV R+WTSA+PP+Q++H VQGDSRIY  PPDP    A+R FR+RYRRF PSDY GWDLWTWD TDP SHR+ L PVAET  WADF+VDRA YGS K I +LPRRGG +WLE+D   R W+ S+  + +   VK    + +     D+DDLPTF ITQ + LVLR LDD  +M++A+ DS++SIVV  P+P  W+ PP+      + D+  K+ + P+    + G ++ D + G++L   K+KQ++P    + FD  VAHF+EDFLVENV+V  P      L WET ++ED+Y YEGSLGW YS+ +C+FRCFAP +  VSVVLY++ TGDAGR VVPMRRIPQGCWKV + +DLKG YYKLLAEG NKRLFPGVEVIDPYSRCNT HTGRGL+YG D T +Y RP ++ +  IVYELHIRD+TIDK SGIR  GKFLGLTERGT +  K  +   +  LT W QE M G + +FS+L+KFSTGLDHI QMGVTA+QIMPIQDFDN+E+D+  YRWGYMPVHFNSPDGWYA+STHT ARV EFK LVSA HKAGL+VIMDVVYNHTAEDSNELNL+ARFSFNGLAPRYYYRTCGNTP+AH G+ TCG R   +P CGECYSNGSGCGNEFRSESPMGRKFIIDSLK+W  E+GVDGFRFDLLGLID++T+  A  ELK I P+I+VYGEPWIGGLSPIK TDKGSQRSKGF VFNDTFRNA+RGSPFDTEETFIMDGGRL EVKGGIIGSVD+F DSP ES+NYVECHDNRTLWDH+ +Y R RTD IKF+EED++RMH+LAAVLVLTSQG+P IQAGQEMCRTKFDDENSYES DEINMIRW  KL EW  VQYYRGLILLRR+HPEIFC+E A+EIH+++ FYEDCGL VP+RCIAYRIKGNPE+L  QL  Q      + +  +E+SL+WSEVVVL NATP+ V FELPG+K D +WMQTV +S AGTR I GP VG++ VSGRSAAVLRRAS  E    QLE+RL+SI+D + SFHGDDPL+RY+VGL+PRPT E+ + RN  ISQR  F +R
Sbjct:    1 MHPSPETPTHLVVHYHRPNHHYHSWTLTLPNATYAQPVQNALTSYGALFRIPFPLPASVTLHPTHPKLVDAPPRQCDLTSLTAVHRELHVFLLEAHPQAVRSP-SLFQTHPNPRLFAISVPDPHAWLIVYARDTKRDVFPASAHYRQRNHPALFLLDTSTLARQIRLTIQHHYHRPAPATAQLSYPAFRAPYPKHVMLSAHPLKAQQTDQDLFAAITTQALQCRFLSRQVQGPTVLDSP----LIRTPKYKHLVLFYYRLDRSTPIVSLTVSASVKLEAVPLPSSYHTSKAFTIDISALPESHSSHS---QLSITPVRSNSGVVIHENKLTWRPSLGLRVIYAQSFPHLLTPAQTDLTLVYHRYDSEWSDWELHVWSKPQDGYPAVSAKLTPTRPVKPGQVVFHLVGYVFPYQAQVFVEPVRMALHPGHATTDEFGITVVGEPYRDACRRDVIRKWTSAQPPSQVIHLVQGDSRIYAHPPDPDTFNAQRRFRLRYRRFLPSDYEGWDLWTWDQTDPESHRIPLTPVAETRDWADFVVDRADYGSGKIISILPRRGGDAWLEQDEFVRQWSLSMLESLDTSIVKH--HEPLKQRADDHDDLPTFLITQETKLVLRHLDDARTMLDAYVDSDNSIVVSAPVPLQWLMPPQLTQSQPNEDMVIKIAQRPVQGLLDVGKEA-DDIEGKSLRFLKAKQVTPIKTGYVFDKSVAHFEEDFLVENVVVSVPGFVSVALHWETHENEDKYLYEGSLGWEYSSAKCIFRCFAPIASLVSVVLYDEPTGDAGRTVVPMRRIPQGCWKVTIIEDLKGRYYKLLAEGGNKRLFPGVEVIDPYSRCNTGHTGRGLVYGIDSTEVYPRPNVKPSAAIVYELHIRDITIDKCSGIRQRGKFLGLTERGTGLPNKVSKSESKV-LTQWPQERMRGVNDEFSLLDKFSTGLDHIVQMGVTAVQIMPIQDFDNDESDDGSYRWGYMPVHFNSPDGWYASSTHTVARVVEFKHLVSALHKAGLKVIMDVVYNHTAEDSNELNLDARFSFNGLAPRYYYRTCGNTPIAHTGDRTCGVREHPKPHCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWAMEYGVDGFRFDLLGLIDLETIHRAATELKSIHPHILVYGEPWIGGLSPIKITDKGSQRSKGFGVFNDTFRNAVRGSPFDTEETFIMDGGRLPEVKGGIIGSVDLFTDSPLESLNYVECHDNRTLWDHLTYYTRSRTDNIKFNEEDLRRMHRLAAVLVLTSQGVPFIQAGQEMCRTKFDDENSYESSDEINMIRWMTKLNEWSTVQYYRGLILLRRSHPEIFCLETADEIHKKVAFYEDCGLCVPERCIAYRIKGNPERLFRQL--QEEEPDVDRDFHREESLRWSEVVVLLNATPLHVTFELPGRKTDTLWMQTVDSSAAGTRIIQGPHVGSVTVSGRSAAVLRRASPTEQEVGQLEMRLSSISDAYSSFHGDDPLTRYTVGLDPRPTVEDVSQRNDLISQRLEFDRR 1492          

BLAST of Gvermi6751.t1 vs. uniprot
Match: R7Q3N6_CHOCR (Pullulanase, Pul1 n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7Q3N6_CHOCR)

HSP 1 Score: 1424 bits (3687), Expect = 0.000e+0
Identity = 752/1528 (49.21%), Postives = 972/1528 (63.61%), Query Frame = 0

Query:    5 SSPAAYLVVHYHRPHHDYHRWLLSVGSRSYAQCVP---------DAFTAYGALFRLPLPP--SSQLLLQPTHPAL-VDSTPRKLDLSSLRSVHHEKHVFLLSDHPHAFASP----LPSSQLASHPFLFVTADFGPEWLIVYGSATKRDVFPATSHYRVPAHPSLFVLDTSRLPNTISFALQKSYHHPAPSHSQPS--FPTFDAPFPEHISLSSR-------PLKPSID--PLSLLLQ-QASR--STYVSPSIPSTTLVSSPKPSPIIRPSKQIILFYFRLDRSTPIVSIML----TASTTLEPVSLPSPYHSARAFSIDVTKLLQLHNSKTNSPQFSIVPIRSSAASTVQEPPLVWTPSLGFRVIYAQSYPYLLTPSRVDITVVYHRFDTH--WADWELHVWTNATEQYPAFTTKLTPRRPSVPGQIVFHLIGHIFPYKTRVFVEPVRMHLHPAKESVDEFGHTVVAEPFRDACRRDVTREWTSAEPPAQILHFVQGDSRIYVQPPDPHQLIAKRWFRIRYRRFSPSDYNGWDLWTWDPTDPSSHRVALAPVAE--TSGWADFIVDRACYGSAKDICLLPRRGGSSWLEKDGVERVWNASLFGNDNEVRVKSITSDFVS-------PGDTDYDDLPTFTITQGSSLVLRSLDDVMSMMEAFADSEHSIVVRTPLPWGWICPPKKAGVASHPDVHFKLCKHPIHMSSNEGNQSFDHMAGRTLCHQKSKQLSPTSIRFFFDDKVAHFDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGDAGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEGENKRLFPGVEVIDPYSRCNTAHTGRGLIYGTDMTPIYDRPKLRVADNIVYELHIRDVTIDKFSGIRNGGKFLGLTERGTKMM-LKSGEHPHQT---PLTPWEQEIMPGADKDFSILNKFSTGLDHIAQMGVTAIQIMPIQDFDNNEADESEYRWGYMPVHFNSPDGWYATSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIRMRTDTIKFSEEDIKRMHKLAAVLVLTSQGIPLIQAGQEMCRTKFDDENSYESPDEINMIRWDAKLKEWPIVQYYRGLILLRRAHPEIFCMENAEEIHERIVFYEDCGLHVPDRCIAYRIKGNPEQLLTQLRKQNSRSGCEEEDLKEQSLQWSEVVVLFNATPVQVVFELPGKKADVMWMQTVSASVAGTRTISGPFVGAIEVSGRSAAVLRRASALECCSSQLELRLASIADVHCSFHGDDPLSRYSVGLEPRPTAEE 1483
            S     LV+HYHR   D+HRW L + +   +   P            T++G  FR+PL    S  + + PTH  L  D+ PR L + +  S   + H+F++      +  P    LPS        L ++      W+++    TK ++FP +S +R P  P L++LD + L  +         H  A   + PS    T D P      +  R        L P +   PL LLL  QA R  S  +  S PST  +  P+   +    K + ++  +L R+   V + L    ++  TL P  L +       F +++ KL    + +  S    IVP+      T      +WTP +G  V+ A+S+  L+    V+++V+YHRF     W DWELHVWT A +++PA++T L P R  +PG   F+  G++FP    V  EPVRM L P +   +E G     EP+ DA RRDV REW S   PA +LHF+QG S +    P    +   R FR+RYRR+ P DY+ WDLWTWD +DP ++R+A+AP+ +  T  W DF +DRA YG+   I L+PR+    W E+D   RVW   +    +     ++ +D  S       P   D+  +P FTI QGSSL+LR L++V SMM A+ DSE SI++ +P+P  W+ PP +    +  D   ++C    +   +      D   G  L  +K  +++PT  R  F++K   F+EDFL+ENV V  P  +   L W   +D D+Y Y G+LGW Y   +C F+CFAP +D + V+LY    G  GR  VPMRRIP+GCWK IV +DLKG YYKL A GEN RLFPGVEVIDPYSRCNT+HTGRGLI+G + T I   P +  ++ I+YELH+RD+TID+ S +   GKFLG+TERGTKM  + SG     T   PLTPW+QE++P  +     L+KFS+ LDHI QMGV  IQI+PIQDFDN+E D+S Y WGYMPVHFNSPDGWYA+ST + ARV EFK+LV A HKAGL+VIMDVVYNHTAEDSNE NL+ARFSFNGLAPRYYYRTCGNTPVA+ G+STC  R P EPRCG CYSNGSGCGNE RSESPMGRKFIIDSLKYWVTE+ VDGFRFDLLGLIDV T+++A  EL+ I+PN+++YGEPW GGLSPI+ T KG QRS+GF VFN+TFR+AIRGSPF TEETF+MDG RL E+KGGIIGSVD F D P E++NYVECHDN TLWDH + YIR RTD I F+E D++RMH+LAA +V TSQGIP +Q GQEMCRTKFD ENSYESPD+INM+RW+AK  EW  VQYYRGLILLRRAHPE+FC   A+EIHE +VFYED GL VP+RCI YRI GN  +L  +L   N     + E L+E+S++W+EVVVL N TP QVVFELP  + D +W++ V A+ +GTR +  P +GAIEV GRSA VLRRAS  E    QL LRL SI+D + SFHGDDP+++Y+VGL  + + +E
Sbjct:    3 SQSRGSLVIHYHRTQGDHHRWDLFLSASQSSPFTPIKVISPVCESGVTSFGTAFRIPLSALSSCSISVLPTHAILGTDNPPRLLTVPTTTS--SDLHIFIVQGDSAVYGDPFAFELPSIASPRFVGLSLSDSSSNPWIVLLNGGTKCELFPVSSPFRDPQAPPLYLLDQAVLAGSAVSIATVVEHSDAVCIAGPSNKHATLDLPVTSASRIQLRRDDNGNLQLHPDLTTTPLPLLLAAQADRCPSLSIIRSAPST--IPPPECVHVPPKGKPLRVYLKQLGRNVYNVKVDLLVTDSSRMTLLPQRLETLDDGRPIFDVNLVKL----SPEKISKGLRIVPVVGGKIGTA----FLWTPEMGLDVVLAESFSELIKIEHVNLSVMYHRFGDFEDWNDWELHVWTEANDRHPAYSTMLKPERSILPGIARFNFDGYLFPCGAVVRAEPVRMALFPGRMLTNEHGEQYQGEPYTDALRRDVVREWVSGSAPAAVLHFLQGHSEVLTDAPLAADMRYGRKFRLRYRRYLPEDYDEWDLWTWDESDPENNRIAVAPMPDSITRAWVDFEIDRARYGAGASISLVPRKNREMWSERDEPTRVWRNDMCNRTSRTLDVALVTDAPSTEIGGECPFQEDFS-VPMFTIVQGSSLILRKLEEVKSMMTAYVDSEESIIIASPVPVAWVSPPNRGRSQAISDTVVRVCSAATNGFRSADGHIEDDTTGIVLPIRKFHEVAPTQFRLTFNEKAVRFEEDFLIENVSVTVPGFDSVRLTWRIHEDWDKYHYTGTLGWEYEVTKCSFKCFAPTADNMYVILYNSPHGKEGRRAVPMRRIPEGCWKAIVEEDLKGKYYKLRAGGENPRLFPGVEVIDPYSRCNTSHTGRGLIFGFEKTAIQPGPCIPASETIIYELHVRDLTIDENSSVIKRGKFLGVTERGTKMKAVSSGSGTGDTSAKPLTPWKQELIPTLEVGMQSLDKFSSALDHIVQMGVNTIQILPIQDFDNDENDDSSYGWGYMPVHFNSPDGWYASSTSSVARVTEFKQLVDAVHKAGLKVIMDVVYNHTAEDSNEYNLDARFSFNGLAPRYYYRTCGNTPVAYTGDSTCAMRKPDEPRCGACYSNGSGCGNELRSESPMGRKFIIDSLKYWVTEYKVDGFRFDLLGLIDVDTLEMAGSELRQINPNVMIYGEPWAGGLSPIRITAKGCQRSRGFGVFNNTFRDAIRGSPFGTEETFVMDGSRLTEIKGGIIGSVDDFCDFPTETVNYVECHDNYTLWDHFRFYIRSRTDDIVFTENDMRRMHRLAAAIVFTSQGIPFMQLGQEMCRTKFDVENSYESPDKINMVRWEAKQSEWTTVQYYRGLILLRRAHPELFCKSTADEIHEGLVFYEDLGLAVPERCIGYRILGNSAKLYQRLLTTNP--DADYEALREESMKWTEVVVLLNPTPSQVVFELPDHERDAIWVEVVDATHSGTRGLRMPMIGAIEVQGRSACVLRRASEREEVDLQLRLRLNSISDCYSSFHGDDPITKYAVGLSSQRSEKE 1515          

BLAST of Gvermi6751.t1 vs. uniprot
Match: A0A7S3E8R2_9RHOD (Pullulanase n=4 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3E8R2_9RHOD)

HSP 1 Score: 915 bits (2366), Expect = 3.100e-306
Identity = 506/1162 (43.55%), Postives = 664/1162 (57.14%), Query Frame = 0

Query:  326 WTPSLGFRVIYAQSY--PYLLTPSRVDITVVYHRFDTHW-ADWELHVWTNATEQYPAFTTKLTP---RRPSVPGQIVFHLIGHIFPYKTRVFVEPVRMHLHPAKESVDEFGHTVVAEPFRDACRRDVTREWTSAEPPAQILHFVQGDSRIYVQPPDPHQLIAKRWFRIRYRRFSPSDYNGWDLWTWDPTDPSSHRVALAPVA-ETSGWADFIVDRACYGSAKDICLLPRRGGSSWLEKDGVERVWNASLFGNDNEVRVKSITSDFVSPGDTDYDDLPTFTITQGSSLVLRSLDDVMSMMEAFADSEHSIVVRTPLPWGWICPPKKAGVASHPDVHFKLCKHPIHMSSNEGNQSFDHMAGRTLCHQKSKQLSPTSIRFFFDDKVAHFDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGD---AGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEGENKRLFPGVEVIDPYSRCNTAH--TGRGLIYGTDMTPIYDRPKLRVADNIVYELHIRDVTIDKFSGIRNGGKFLGLTERGTKMMLKSGEHPHQTPLTPWEQEIMPGADKDFSILNKFSTGLDHIAQMGVTAIQIMPIQDFDNNEADESEYRWGYMPVHFNSPDGWYATSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIRMRTDTIKFSEEDIKRMHKLAAVLVLTSQGIPLIQAGQEMCRTKFDDENSYESPDEINMIRWDAKLKEWPIVQYYRGLILLRRAHPEIFCMENAEEIHERIVFYEDCGLHVPDRCIAYRIKGNPEQLLTQLRKQNSRSGCEEEDLKEQSLQWSEVVVLFNATPVQVVFELPGKKADVMWMQTVSASVAGTRTISGPFVGAIEVSGRSAAVLRRASALECCSSQLELRLASIADVHCSFHGDDPLSRYSVGL 1475
            W P  G+ V   +    P    P    IT  YHR      + W+L V  +  ++    +  + P        PG  +F L    FP  +++ + PV+      +E ++  G           C R+V  +    E     +H  QG   ++   PD   L  +R+F + YRR+   D  GWD+WTWD  D  S  VA  PV  E + +  F VDR+ YGS ++I L+ R+GG  W  KD  +R+W A           +    +FV P          +   QG   ++R L+D +  + A+ +++ +I + + +   W           HP++     K  + + S  G         + +   K ++ S        D +    DEDF VE+  + A    P  L W  + + D YFYEG LG  +   +C FR FAP +D VSVV+Y+ A G     GR V PMRRIPQGCWK  +  +LKG YYKLLAEG +KRL+PGVEVIDPYSRCNTAH   GRGLIY  D T I DRPK   + +++YELH+RD TID  SG+   GKF+GLTERGT               T W+++             K ST L+HI +MGV  +QIMPIQDFDN+E  + EYRWGYMPVHFNSPDGWYA+ST   +RV EFKKLV   H  G++V+MDVVYNHT+ED NE NLEARFSFNGLAPRYYYR C + PVA +G +TC      EP CG CYSNGSGCGNEFRS++PMGRKFIIDSLK+WV E+ +DGFRFDL+GLID +T+  A  ELK ID  I++YGEPW GG SPI  T KG QR +GF VFND+ R+A+RG  +  +ETF+MDGG   +VK GI+GS++ FA  P E+INY ECHDNRTLWD   HY  +RTD+I F++ D  RM KLAA+++ TSQG+P IQ GQEMCRTK   ENSYESPD +NM++W  K +    V+YY+GLI +R AH  +F M  A +I + ++F+E+ GL  P+RCIA+RI  +  +  ++ R                   WS+VVVL N TP++  F LP +  D +W        AGT TI  P    I V GRS  VLRR S +E  +    +R AS           D  SR S+GL
Sbjct:  315 WQPEFGYHVFLLEGMEGPVTFEPRPRQIT--YHRLGVPVDSSWQLEVTCDEWKKLAGSSQAIVPVPALSEKQPG--LFELSDFWFPPSSKIMIRPVK-----TREDLETDGPESTKLYDMTDCWREVPEDRLEGE-----MHLSQGYLGLFQDEPDQKVLNRERYFYVEYRRYD-DDGAGWDIWTWDNDDDFSDPVAAQPVDWENAAYPTFRVDRSLYGSGREILLIGRQGGEHWTAKDEPQRMWKA-----------EGRRPNFVDP----------YIFLQGHPHLVRRLEDAIGNLSAYVETDGTISLHSSMDLKWF----------HPNIDDSSKKSSLIIKSANG---------KAMKIVKVERKSSVLRVIHVDIQSQLLDEDFRVEDFTISARNFSPEVLTWRRYDNFDEYFYEGDLGLEFGIAECTFRTFAPGADHVSVVIYDTAVGPNGGGGRKVHPMRRIPQGCWKANIKANLKGKYYKLLAEGSDKRLYPGVEVIDPYSRCNTAHHTEGRGLIYPMDNTRIADRPKFPTSASVIYELHLRDSTIDPASGVNKKGKFMGLTERGTST-------------TVWDEKTGQNV--------KISTALEHILEMGVNTVQIMPIQDFDNDENSQEEYRWGYMPVHFNSPDGWYASSTSNFSRVVEFKKLVDCFHSNGIKVVMDVVYNHTSEDPNEFNLEARFSFNGLAPRYYYRNCVSHPVAASGHNTCAWHGMDEPHCGTCYSNGSGCGNEFRSDAPMGRKFIIDSLKFWVEEYKIDGFRFDLMGLIDQETMTQATAELKKIDDGILIYGEPWTGGPSPIVATVKGFQRDRGFGVFNDSLRDALRGGNWGIQETFLMDGGEAEKVKRGIMGSIEDFASQPTETINYAECHDNRTLWDQFSHYRELRTDSIVFTDSDFIRMGKLAALIIFTSQGLPFIQIGQEMCRTKGGVENSYESPDSVNMVQWKWKQERRRCVEYYKGLIKMRLAHQHLFAMTYASDIRKNLIFFEELGLQPPERCIAFRISSDHPEGPSKAR-------------------WSQVVVLLNPTPLEQRFWLPNEP-DFLWGPVADDVAAGTDTIGAPVQDQISVRGRSGCVLRRISRVEEPAYDATIR-ASFVSSPFESGITDAASRNSLGL 1379          

BLAST of Gvermi6751.t1 vs. uniprot
Match: A0A5J4YVH4_PORPP (Pullulanase n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YVH4_PORPP)

HSP 1 Score: 878 bits (2269), Expect = 1.130e-286
Identity = 491/1122 (43.76%), Postives = 648/1122 (57.75%), Query Frame = 0

Query:  393 GQIVFHLIGHIFPYKTRVFVEPVRMHLHPAKESVDEFGHTVVAEPFRDACRRDVTREWTSAEPPAQILHFVQGDSRIYVQPPDPHQLIAKRWFRIRYRRFSPSDY---NGWDLWTWDPTDPSSHRVALAPV-AETSGWAD---------------------FIVDRACYGSAKDICLLPRRGGSSWLEKDGVERVWNASLFGNDNEVRVKSITSDFVSPGDTDYDDLPTFTITQGSSLVLRSLDDVMSMMEAFADSEHSIVVRTPLPWGWICPPKK-AGVASHPDVHFKLCKHPIHMSSNEGNQSFDHMAGRTLCHQKSKQLSPTSIRFFFDDKVAHFDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGDAGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEG--ENKRLFPGVEVIDPYSRCNTAHTGRGLIYGTDMTPIYDRPKLRVADNIVYELHIRDVTIDKFSGIRNGGKFLGLTERGTKMMLKSGEHPHQTPLTPWEQEIMPG-----------ADK---------------------------------DFSILNKFSTGLDHIAQMGVTAIQIMPIQDFDNNEA--DESEYRWGYMPVHFNSPDGWYATSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIRMRTDTIKFSEEDIKRMHKLAAVLVLTSQGIPLIQAGQEMCRTKFDDENSYESPDEINMIRWDAKLKEWPIVQYYRGLILLRRAHPEIFCMENAEEIHERIVFYEDCGLHVPDRCIAYRIKGNPEQLLTQLRKQNSRSGCEEEDLKEQSLQWSEVVVLFNATPVQVVFELPGKKADVMWMQTVSASVAGT-RTISGPFVGAIEVSGRSAAVLRRAS 1439
            G+ +F      FP    ++V+PV + L        + G  +VA         D  + W        I H VQG   +          +A     I Y R   S       WDLW WD  D +   +   P   E S  +D                     F + RAC G    I LLPR GG++W ++      W+ +    D                           + +G   ++  L D +  M A    ++ + + T LP  W  P +  A V +            +   ++ G+Q+   +  + L    S  +   ++R  F+ +   FDEDF VE   V    +  A LRW  +++ED Y+Y+G+LGW YS  + VFRCFAP++ ++SVVLY   +G  GR++V MRRI  G WK  +  DLKG YYKLLA    ++ R FPGVEVIDPYSRCNT+HTGRGLI+G D TP+ DRP +   + I+YE+H+RD+TID+ SGI   GKFLGLTERG+  ML+ GE   Q P      E   G           AD                                  D   +   ST LDHI +MGVTA+QI+P+QDFDN+E+  D ++Y WGYMPVHFNSPDGWYAT T +AARV EFK+LVSA H  GL+VIMDVVYNHTAED+NE NLEARFSFNGLAPRYYYR CGNTP++    +TC  R  HE RCG C+SNGSGCGNEFRSE+PMGRKF++DSL+YWV+E+ VDGFRFDLL L+D +TV +   E++ IDPNI++YGEPW GG++PI  T+KG+QR +GF+VFNDTFR+A+RGS +  +ETF+MDGGR+ EVK GI+GS+D+FADSP ESINY+ECHDNRTL+D  +HY+  R D I +++ D +RM +L A++VLTSQG+P I AGQEM RTK   ENSY SPD+INM+RW  K +    V+Y++ LI LRR HP++F M NA E+ ERI F+ED  L VP+RCIAY ++G  E       + +S S  E   +++Q   WS+VVVL N TP+   F LPG      W+  V    A   R +  P +  + V GRS  +LR A+
Sbjct:  722 GRYLFDTSVAHFPQGRAIYVQPVLLDL--------DTGTPLVA---------DCVKLWPERTHGPDISHAVQGARDVXXXXXXXXDALATPNVGIMYLRHGVSTRAHARQWDLWVWDAEDETVAALLPDPADKEPSSSSDGFGFGQPHMDVVNIDGLSFVVFSIPRACLGCGNRIKLLPRHGGAAWTDRGAHTVTWDCTRHPRDR-------------------------MLLEGFPELVAELFDAVGDMTASVIDDNIVEISTSLPASWFAPEQDPANVKA------------VFKGASSGHQNVLDL-NKVLLSMTS--VDAWTMRATFEPRAVRFDEDFPVELYSVSVLNMHDARLRWPVYENEDAYYYDGTLGWEYSPSETVFRCFAPSAAELSVVLYSAPSGQQGRELVSMRRIMGGVWKARIQGDLKGRYYKLLALSTPDDTRRFPGVEVIDPYSRCNTSHTGRGLIFGRDTTPVTDRPNVPPQNAIIYEIHVRDMTIDQQSGILMRGKFLGLTERGS--MLQEGE---QEPSAGQNCETKVGTVTEQARKLGVADSVAARASPSKRAQKAENGVSQGEQLDHLPLPDIIDDVEITPLSTALDHIIEMGVTAVQILPVQDFDNDESSTDGNQYWWGYMPVHFNSPDGWYATDTTSAARVSEFKQLVSALHDVGLKVIMDVVYNHTAEDANEFNLEARFSFNGLAPRYYYRNCGNTPLSVGDHNTCANRGKHEKRCGTCFSNGSGCGNEFRSEAPMGRKFLLDSLRYWVSEYKVDGFRFDLLALVDTETVAMLAREMQKIDPNIMLYGEPWTGGMTPIAPTEKGTQRGRGFSVFNDTFRDALRGSTWSVDETFLMDGGRIDEVKRGILGSIDVFADSPLESINYIECHDNRTLYDQFQHYVANRQDDITYTDADYERMARLGALIVLTSQGVPFIHAGQEMMRTKQGVENSYCSPDDINMVRWAWKRERLMCVRYHQHLIRLRRTHPQLFSMTNAAEVRERITFFEDLDLIVPERCIAYHMQGYKEGEYAHGYRIDSLSSEERRRIEQQ---WSQVVVLLNPTPMGTDFGLPGAAHQQAWVCMVDDEFASCERAVRAPVMIQVHVPGRSGMLLRAAA 1778          

BLAST of Gvermi6751.t1 vs. uniprot
Match: A0A7S3E758_9RHOD (Hypothetical protein n=1 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3E758_9RHOD)

HSP 1 Score: 798 bits (2061), Expect = 4.130e-267
Identity = 429/955 (44.92%), Postives = 558/955 (58.43%), Query Frame = 0

Query:  326 WTPSLGFRVIYAQSY--PYLLTPSRVDITVVYHRFDTHW-ADWELHVWTNATEQYPAFTTKLTP---RRPSVPGQIVFHLIGHIFPYKTRVFVEPVRMHLHPAKESVDEFGHTVVAEPFRDACRRDVTREWTSAEPPAQILHFVQGDSRIYVQPPDPHQLIAKRWFRIRYRRFSPSDYNGWDLWTWDPTDPSSHRVALAPVA-ETSGWADFIVDRACYGSAKDICLLPRRGGSSWLEKDGVERVWNASLFGNDNEVRVKSITSDFVSPGDTDYDDLPTFTITQGSSLVLRSLDDVMSMMEAFADSEHSIVVRTPLPWGWICPPKKAGVASHPDVHFKLCKHPIHMSSNEGNQSFDHMAGRTLCHQKSKQLSPTSIRFFFDDKVAHFDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGD---AGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEGENKRLFPGVEVIDPYSRCNTAH--TGRGLIYGTDMTPIYDRPKLRVADNIVYELHIRDVTIDKFSGIRNGGKFLGLTERGTKMMLKSGEHPHQTPLTPWEQEIMPGADKDFSILNKFSTGLDHIAQMGVTAIQIMPIQDFDNNEADESEYRWGYMPVHFNSPDGWYATSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIRMRTDTIKFSEEDIKRMHKLAAVLVLTSQGIPLIQAGQEMCRTK 1268
            W P  G+ V   +    P    P    IT  YHR      + W+L V  +  ++    +  + P        PG  +F L    FP  +++ + PV+      +E ++  G           C R+V  +    E     +H  QG   ++   PD   L  +R+F + YRR+   D  GWD+WTWD  D  S  VA  PV  E + +  F VDR+ YGS ++I L+ R+GG  W  KD  +R+W A           +    +FV P          +   QG   ++R L+D +  + A+ +++ +I + + +   W           HP++     K  + + S  G         + +   K ++ S        D +    DEDF VE+  + A    P  L W  + + D YFYEG LG  +   +C FR FAP +D VSVV+Y+ A G     GR V PMRRIPQGCWK  +  +LKG YYKLLAEG +KRL+PGVEVIDPYSRCNTAH   GRGLIY  D T I DRPK   + +++YELH+RD TID  SG+   GKF+GLTERGT               T W+++             K ST L+HI +MGV  +QIMPIQDFDN+E  + EYRWGYMPVHFNSPDGWYA+ST   +RV EFKKLV   H  G++V+MDVVYNHT+ED NE NLEARFSFNGLAPRYYYR C + PVA +G +TC      EP CG CYSNGSGCGNEFRS++PMGRKFIIDSLK+WV E+ +DGFRFDL+GLID +T+  A  ELK ID  I++YGEPW GG SPI  T KG QR +GF VFND+ R+A+RG  +  +ETF+MDGG   +VK GI+GS++ FA  P E+INY ECHDNRTLWD   HY  +RTD+I F++ D  RM KLAA+++ TSQG+P IQ GQEMCRTK
Sbjct:   28 WQPEFGYHVFLLEGMEGPVTFEPRPRQIT--YHRLGVPVDSSWQLEVTCDEWKKLAGSSQAIVPVPALSEKQPG--LFELSDFWFPPSSKIMIRPVK-----TREDLETDGPESTKLYDMTDCWREVPEDRLEGE-----MHLSQGYLGLFQDEPDQKVLNRERYFYVEYRRYD-DDGAGWDIWTWDNDDDFSDPVAAQPVDWENAAYPTFRVDRSLYGSGREILLIGRQGGEHWTAKDEPQRMWKA-----------EGRRPNFVDP----------YIFLQGHPHLVRRLEDAIGNLSAYVETDGTISLHSSMDLKWF----------HPNIDDSSKKSSLIIKSANG---------KAMKIVKVERKSSVLRVIHVDIQSQLLDEDFRVEDFTISARNFSPEVLTWRRYDNFDEYFYEGDLGLEFGIAECTFRTFAPGADHVSVVIYDTAVGPNGGGGRKVHPMRRIPQGCWKANIKANLKGKYYKLLAEGSDKRLYPGVEVIDPYSRCNTAHHTEGRGLIYPMDNTRIADRPKFPTSASVIYELHLRDSTIDPASGVNKKGKFMGLTERGTST-------------TVWDEKTGQNV--------KISTALEHILEMGVNTVQIMPIQDFDNDENSQEEYRWGYMPVHFNSPDGWYASSTSNFSRVVEFKKLVDCFHSNGIKVVMDVVYNHTSEDPNEFNLEARFSFNGLAPRYYYRNCVSHPVAASGHNTCAWHGMDEPHCGTCYSNGSGCGNEFRSDAPMGRKFIIDSLKFWVEEYKIDGFRFDLMGLIDQETMTQATAELKKIDDGILIYGEPWTGGPSPIVATVKGFQRDRGFGVFNDSLRDALRGGNWGIQETFLMDGGEAEKVKRGIMGSIEDFASQPTETINYAECHDNRTLWDQFSHYRELRTDSIVFTDSDFIRMGKLAALIIFTSQGLPFIQIGQEMCRTK 906          

BLAST of Gvermi6751.t1 vs. uniprot
Match: A0A1X6PAN7_PORUM (Aamy domain-containing protein n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6PAN7_PORUM)

HSP 1 Score: 766 bits (1977), Expect = 1.610e-251
Identity = 468/1163 (40.24%), Postives = 618/1163 (53.14%), Query Frame = 0

Query:  445 DVTREWTSAEPPAQILHFVQGDSRIYVQPPD---------PHQLIAKRWFRIRYRRF--SPSDYNGWDLWTWDP--TDPSSHRVALAPVA-ETSGWADFIVDRACYGSAKDICLLPRRGGSSWLEKDGVERVWNASLFGNDNEVRVKSITSDFVSPGDTDYDDLPTFTITQGSSLVLRSLDDVMSMMEAFADSEHSIVVRTPLPWGWI---CPPKKAGVASHPDVHFKLCKHPIHMSSNEGNQSFDHMAGRTLCHQKSK-------QLSPTSIRFFFDDKVAH---FDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGDAGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEGENKRLFPGVEVIDPYSRCNTAHTGRGLIYGTD----MTPIYDRPKLRVADNIV-YELHIRDVTIDKFSGI-RNGGKFLGLTERGTKMMLKSGEHPHQTPLTPWEQE------IMPGAD-----KDFSILN------KFSTGLDHIAQMGVTAIQIMPIQDFDNNEADES--EYRWGYMPVHFNSPDGWYATSTHT-AARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIR-----MRTDTIKFSEEDI----------------------------------------------------KRMHKLAAVLVLTSQGIPLIQAGQEMCRTKFDDENSYESPDEINMIRWDAKLKEWPIVQYYRGLILLRRAHPEIFCMENAEEIHERIVFYEDCGLHVPDRCIAYRIKGNPEQLLTQLRKQNSRSGCEEEDLKEQSLQWSEVVVLFNATPVQVVFELPGKKADVMWMQTVSASVAGTRTISGPFVGAIEVSGRSAAVLRRASALECCSSQLELRLASIADVHCSFHGDDPLSRYSVGLEPRPTAEEQAIRNQSISQRQRF 1497
            DV R WT           VQG   IY +  +              A RWF +RYRRF      Y GW LW  D      S   VA+ PV+ +    A F+VDR  +G    + L+PR    + ++  G    W+         +    + +     GDT     P   + QG   +L SL    S +    DS   + V T  P  W+    P K   +A+ P    KL                      TL H +++       + SPT +     + VA      ED+LVE V+  A  +    L+W    + D Y+Y G++G   +  + VFR FAP +D V+VVLY+    DA R  VPMRRIPQGCWK IV   L G YYKLLA G +  LFPGVEVIDPYSRCNT HTGRGLI   D    +  +  RP +   D +V +ELH+RD T+D  SG+  + GK+LGLT+R T ++          P  P+  +      +  GAD     K     N        ST L H+ +MG TA+Q++P+QDFDNNE DE+  EY WGYMPVHF SPDGWYA+ + T + RV+E K+LVSA H+AGLRVI+DVV+NHTAED NE NL+ARFSFNG+APRYYYRTCGNTP++ +G  TC       P CG CYSNGSGCGNE RSE+PM RKFIID L+YW TE+G+DGFRFDL+GL+DV T+  A   LK ID  I++YGEPW GG++P+  T KG+QR +GF VFNDTFR+A+RGSPF     F++DG  + EVK GI+GSVD FAD+P E INYV CHDNRTL+D +    R        D     EE +                                                    +R   LA  +VLT+QG+P +Q GQE  RTK  D NSY SPD +N +RW  K      V++  GL+ LRR HPEIF +  A  + ER++F+E  G+ VP  C+AYR+ G    ++ +     + +G       EQ+ +W+ V+VL N       F LP  +A+ +W+  V+   AG   +  P    + V+  S  VLRR SA E  ++ ++ RLA I+D        D  SRY+VGLE    A E A+  +++  R+ F
Sbjct:   11 DVARSWTILPGVVPRRQIVQGHPTIYREASEFLGDGGGDTTVCSAASRWFAVRYRRFVGEADAYAGWVLWGCDDDAVGASVGGVAIEPVSVDPVEGALFLVDRGRFGDGSRLRLVPRHAERA-VDDAGATVTWDVDAADAAFWIPATVVDALVAGGGDTPP---PALLLVQGHGSLLTSLASEPSPVVVSVDSPWDVAVTTFCPLEWLDGTAPAKPWDLAASPSTRPKL----------------------TLRHGQAELPWVTWERPSPTEVVLRLSEAVADGRGLHEDYLVEEVVARATGIPTLALQWRRHANVDAYYYPGAMGCMCTASETVFRVFAPTADGVAVVLYDGPGDDASRREVPMRRIPQGCWKAIVPSSLGGTYYKLLASGADTLLFPGVEVIDPYSRCNTHHTGRGLILAPDDPAVVATVAPRPPVGPDDAVVIWELHLRDATVDPASGVVGHRGKYLGLTQRDTVLV----------PGAPFAADPKGMVDVSAGADAADTAKPAPAANGEADAPPVSTALAHLVEMGATAVQLLPLQDFDNNE-DEAVGEYAWGYMPVHFFSPDGWYASGSRTDSTRVRELKELVSAMHEAGLRVILDVVFNHTAEDVNERNLDARFSFNGVAPRYYYRTCGNTPLSASGHRTCALTLAGAPTCGGCYSNGSGCGNEMRSEAPMARKFIIDCLRYWATEYGMDGFRFDLMGLVDVPTLTAASTALKAIDDGILLYGEPWTGGMTPMAPTYKGTQRGRGFGVFNDTFRDALRGSPFHVSSCFVLDGVCVDEVKRGIMGSVDDFADAPTEVINYVACHDNRTLYDQLWEIYRGGGEDTGADVAPPEEEPVGASAVKDIGPAPDATHLSPDAATAVAXXXXXXXXXXXXXXXXXXXXXVPALLRRCAALATAIVLTAQGVPFLQLGQEFFRTKGGDHNSYISPDAVNAVRWARKAAHAAAVRFVAGLVALRRGHPEIFALPTAGLVRERLLFFETMGVPVPAGCVAYRVDGCAADVVARA----AAAGASAATANEQAARWTAVLVLINPCRTAQAFALP--EAEQLWVPVVAGGHAGLSPVGAPVRRQLGVAPTSLTVLRRCSAAEADAAAVDDRLAVISDPGVVPADGDCTSRYAVGLERERGAAEAAVMEENVRLRRAF 1130          

BLAST of Gvermi6751.t1 vs. uniprot
Match: A0A7V5M1D9_9BACT (Aamy domain-containing protein n=1 Tax=bacterium TaxID=1869227 RepID=A0A7V5M1D9_9BACT)

HSP 1 Score: 739 bits (1907), Expect = 7.100e-246
Identity = 396/805 (49.19%), Postives = 517/805 (64.22%), Query Frame = 0

Query:  690 PTSIRFFFDDKVAHFDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGDAGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEGENKRLFPGVEVIDPYSRCNTAHTGRGLIYGTDMTPIYDRPKLRVADNIVYELHIRDVTIDKFSGI--RNGGKFLGLTERGTKM---------MLKSGE-----HPHQTPLTPWEQEIMPGADKD----FSILNKF---------STGLDHIAQMGVTAIQIMPIQDFDNNEADESEYRWGYMPVHFNSPDGWYATSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIRMRTDT-IKFSEEDIKRMHKLAAVLVLTSQGIPLIQAGQEMCRTKFDDENSYESPDEINMIRWDAKLKEWPIVQYYRGLILLRRAHPEIFCMENAEEIHERIVFYEDCGLHVPDRCIAYRIKGNPEQLLTQLRKQNSRSGCEEEDLKEQSLQWSEVVVLFNATPVQVVFELPGKKADVMWMQTVSASVA---GTRTISGPFVGAIEVSGRSAAVLRRASALECCSSQLELRLASIADVHCS 1461
            P  ++F  D  +   DEDF VE V+VH     P+   W  +++ D Y+Y G+LGW Y+     FR FAP +D V + LY +  G  GR   PMRRIPQG WK++V  DL+G+YY  LAEG NKRLFPGVEVIDPYSRCNTAH GRGLI+G D T + DRP +     I+YE+H+RD TID+ SG+  +  GK+LGL ++GT++         +  +G          TP   +E+++ P   ++      +L +          ST LDH+  +GV A+QIMP+QDFDN+E++  EYRWGYMPVHF SPDGWYA+ T  A+RV+E K+L+   H+AGL+VI+D+V NHTAED NE NLEARFSFNGLAPRYYYR     P    G S              CY+NGSGCGNEFRSESPMGRKFI+D L YW TE+ VDGFRFDL+GL+D +T+++A  +L  +DPNI+VYGEPW  G +PI  T KG+QR  GF VFNDTFRNA+RGS    EE F++DGG +  VK GI+GS+D FA SP E INYVECHDNRTLWDH+ + IR R D  I ++  D+ R+ KLAAV++LTSQGIP + +GQEM R+KF  ENSY+S D+IN +RW+ K+  + +V Y RGL+ LRRA P+IF M +A+++   I FYE  GL VP+RC+AYRI  +  Q         S +   E+D       W  V +LFN TPV  VF LP +  D +    V+   A      ++    +G   V GRSA VLR  +  E   + +E RL +I++ + S
Sbjct:    7 PLKLQFQLDRTL---DEDFPVEEVMVHLKGFSPSRGAWRRYENVDEYYYSGALGWHYTKSATTFRVFAPTADLVVLCLYREPVGTVGRQAFPMRRIPQGVWKIVVHGDLQGLYYTYLAEGVNKRLFPGVEVIDPYSRCNTAHNGRGLIFGEDHTEVADRPCIPPEHTIIYEVHLRDFTIDECSGVPEKLRGKYLGLAQQGTQLPEHWVRSRIVSSAGRAALFLRDRATP-DAFEEDVCPSTVEEDVYTAPVLTEVAAQVDWTDRSTCLDHVRFLGVNALQIMPVQDFDNDESNPLEYRWGYMPVHFFSPDGWYASCTSDASRVRELKQLIDTLHRAGLKVILDMVLNHTAEDPNEFNLEARFSFNGLAPRYYYRFW---PDGSWGVSEA------------CYANGSGCGNEFRSESPMGRKFILDMLWYWATEYKVDGFRFDLMGLMDRETLRIAAAKLHALDPNILVYGEPWSAGETPIIITGKGAQRHLGFGVFNDTFRNALRGSNQGIEENFLLDGGCIEAVKQGILGSIDDFAASPLEVINYVECHDNRTLWDHLWYVIRERADANITYTAADVLRVCKLAAVILLTSQGIPFLHSGQEMARSKFGVENSYQSGDDINRLRWERKIDFYGLVVYVRGLVQLRRARPDIFSMSDAQQVRRCIAFYEALGLPVPERCLAYRIIADAGQ---------SETNAIEDD------PWRCVALLFNPTPVAQVFPLPPEAKDELMTVIVNDLEAPRDAGASLGEYHIGWASVPGRSAMVLRLCTESERERALIETRLNAISEPYSS 777          

BLAST of Gvermi6751.t1 vs. uniprot
Match: M1VKA6_CYAM1 (Pullulanase n=1 Tax=Cyanidioschyzon merolae (strain 10D) TaxID=280699 RepID=M1VKA6_CYAM1)

HSP 1 Score: 738 bits (1905), Expect = 1.650e-233
Identity = 396/805 (49.19%), Postives = 517/805 (64.22%), Query Frame = 0

Query:  690 PTSIRFFFDDKVAHFDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGDAGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEGENKRLFPGVEVIDPYSRCNTAHTGRGLIYGTDMTPIYDRPKLRVADNIVYELHIRDVTIDKFSGI--RNGGKFLGLTERGTKM---------MLKSGE-----HPHQTPLTPWEQEIMPGADKD----FSILNKF---------STGLDHIAQMGVTAIQIMPIQDFDNNEADESEYRWGYMPVHFNSPDGWYATSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIRMRTDT-IKFSEEDIKRMHKLAAVLVLTSQGIPLIQAGQEMCRTKFDDENSYESPDEINMIRWDAKLKEWPIVQYYRGLILLRRAHPEIFCMENAEEIHERIVFYEDCGLHVPDRCIAYRIKGNPEQLLTQLRKQNSRSGCEEEDLKEQSLQWSEVVVLFNATPVQVVFELPGKKADVMWMQTVSASVA---GTRTISGPFVGAIEVSGRSAAVLRRASALECCSSQLELRLASIADVHCS 1461
            P  ++F  D  +   DEDF VE V+VH     P+   W  +++ D Y+Y G+LGW Y+     FR FAP +D V + LY +  G  GR   PMRRIPQG WK++V  DL+G+YY  LAEG NKRLFPGVEVIDPYSRCNTAH GRGLI+G D T + DRP +     I+YE+H+RD TID+ SG+  +  GK+LGL ++GT++         +  +G          TP   +E+++ P   ++      +L +          ST LDH+  +GV A+QIMP+QDFDN+E++  EYRWGYMPVHF SPDGWYA+ T  A+RV+E K+L+   H+AGL+VI+D+V NHTAED NE NLEARFSFNGLAPRYYYR     P    G S              CY+NGSGCGNEFRSESPMGRKFI+D L YW TE+ VDGFRFDL+GL+D +T+++A  +L  +DPNI+VYGEPW  G +PI  T KG+QR  GF VFNDTFRNA+RGS    EE F++DGG +  VK GI+GS+D FA SP E INYVECHDNRTLWDH+ + IR R D  I ++  D+ R+ KLAAV++LTSQGIP + +GQEM R+KF  ENSY+S D+IN +RW+ K+  + +V Y RGL+ LRRA P+IF M +A+++   I FYE  GL VP+RC+AYRI  +  Q         S +   E+D       W  V +LFN TPV  VF LP +  D +    V+   A      ++    +G   V GRSA VLR  +  E   + +E RL +I++ + S
Sbjct:  991 PLKLQFQLDRTL---DEDFPVEEVMVHLKGFSPSRGAWRRYENVDDYYYSGALGWHYTKSATTFRVFAPTADLVVLCLYREPVGTVGRQAFPMRRIPQGVWKIVVHGDLQGLYYTYLAEGVNKRLFPGVEVIDPYSRCNTAHNGRGLIFGEDHTEVADRPCIPPEHTIIYEVHLRDFTIDECSGVPEKLRGKYLGLAQQGTQLPEHWVRSRIVSSAGRAALFLRDRATP-DAFEEDVCPSTVEEDVYTAPVLTEVAAQVDWTDRSTCLDHVRFLGVNALQIMPVQDFDNDESNPLEYRWGYMPVHFFSPDGWYASCTSDASRVRELKQLIDTLHRAGLKVILDMVLNHTAEDPNEFNLEARFSFNGLAPRYYYRFW---PDGSWGVSEA------------CYANGSGCGNEFRSESPMGRKFILDMLWYWATEYKVDGFRFDLMGLMDRETLRIAAAKLHALDPNILVYGEPWSAGETPIIITGKGAQRHLGFGVFNDTFRNALRGSNQGIEENFLLDGGCIEAVKQGILGSIDDFAASPLEVINYVECHDNRTLWDHLWYVIRERADANITYTAADVLRVCKLAAVILLTSQGIPFLHSGQEMARSKFGVENSYQSGDDINRLRWERKIDFYGLVVYVRGLVQLRRARPDIFSMSDAQQVRRCIAFYEALGLPVPERCLAYRIIADAGQ---------SETNAIEDD------PWRCVALLFNPTPVAQVFPLPPEAKDELMTVIVNDLEAPRDAGASLGEYHIGWASVPGRSAMVLRLCTESERERALIETRLNAISEPYSS 1761          

BLAST of Gvermi6751.t1 vs. uniprot
Match: A0A1Y1RKU5_9BACT (Pullulanase n=1 Tax=Candidatus Cloacimonetes bacterium 4572_55 TaxID=1971726 RepID=A0A1Y1RKU5_9BACT)

HSP 1 Score: 635 bits (1638), Expect = 1.850e-205
Identity = 376/956 (39.33%), Postives = 505/956 (52.82%), Query Frame = 0

Query:  486 RIRYRRFSPSDYNGWDLWTWDPTDPSSHRVALAPVAETSGWA-DFIVDRACYGSAKDICLLPRRGGSSWLEKDGVERVWNASLFGNDNEVRVKSITSD-FVSPGDTDYDDLPTFTITQGSSLVLRSLDDVMSMMEAFADSEHSIVVRTPLPWGWICPPKKAGVASHPDVHFKLCKHPIHMSSNEGNQSFDHMAGRTLCHQKSKQLSPTSIRFFFDDKVAHFDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGDAGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEGENKRLFPGVEVIDPYSRCNTAHTGRGLIYGTDMTPIYDRPKLRVADNIVYELHIRDVTIDKFSGIRNGGKFLGLTERGTKMMLKSGEHPHQTPLTPWEQEIMPGADKDFSILNKFSTGLDHIAQMGVTAIQIMPIQDFDNNEADESEYRWGYMPVHFNSPDGWYATSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDT---EETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIRMRTDTIKFSEEDIKRMHKLAAVLVLTSQGIPLIQAGQEMCRTKFDDENSYESPDEINMIRWDAKLKEWPIVQYYRGLILLRRAHPEIFCMENAEEIHERIVFYE-DCGLHVPDRCIAYRIKGNPEQLLTQLRKQNSRSGCEEEDLKEQSLQWSEVVVLFNATPVQVVFELPGKKADVMWMQTVSASVAGTRTISGPFVGAIEVSGRSAAVL 1435
            R+ Y R+   +Y GW+LWTWD       R A+ P+ +   +   F++D+  YGS + I LLPR+G   WLEKD  +R+W  +L    NE  +     D FV P DT                          +  AF D+   + V  P+            +   P+      +H + +  ++G                +  L  TS      D    FD    +    V     +P  L +    D   Y  +  LG  Y   Q +FR FAP + +V++ +Y++   D G+   P+     G W++ + ++LK  YY    +G +    P  E+IDPYSRCNTAH GRG+I   D T I DRP     D I+YELH+RD +ID  SGI   G +LG+TE+G                              +    +  TGLDH+ +MGV  +QIMPIQDFDNNE+ E EY WGYMPVHFNSPDGWYAT    A RV+EFKKLV A H  G+RVIMDVVYNHTAE     N   ++SFNG+AP YYYR                       R    + NGSGCGNEFRSE+PM RKFI+DS+ YWV E+ VDGFRFDL+GLID++T+    E L  IDPNI+VYGEPW  G +PI  T KG QR  GF VFND FR+A++GSPFD     ETF++ G R+ E+K GI GS+  FA  P ES+NY+ECHDNRTLWD + +  + +TD       DI +M KL AV++ TSQG+P IQ GQEM RTK D ENSY +PD +N I W+ K++   +V+Y++GLI LR+AH ++F   + + I + + F + D G+ VPD+CIAY+                         L+++  +W  V+VL N  P+ V+F +P       W   V  + +G   I         V+ RSAAVL
Sbjct:   28 RVHYHRYD-GNYEGWNLWTWDKVSNDYSR-AIQPLKKRDHYGLTFVLDKTHYGSGEMIGLLPRKG--EWLEKDDPDRIWTPNL---GNEAWILQNDPDLFVEPPDT-----------------------YPQISGAFIDAPRRVTVCLPI-----------SIRFDPEQ----AEH-VRIIDDQGQPIPIQSVAPGEIGNAALLLINTS------DSFRAFDLAMPIHKYRVEVLGFKPKGLSFRMLMDRPEYVSDVELGAIYKPDQTLFRLFAPTATRVTLTIYDQPDRDIGQ-AYPLESQASGVWEIDIKQNLKNKYYTYKVDGNSPLFHPDQELIDPYSRCNTAHNGRGMIID-DGTRIADRPDFPPEDAIIYELHVRDFSIDTDSGINQKGLYLGMTEQGAT----------------------------YKDQGEVKTGLDHLVEMGVNTVQIMPIQDFDNNESSE-EYNWGYMPVHFNSPDGWYATEQRNAKRVEEFKKLVDALHTRGIRVIMDVVYNHTAET----NPYVQYSFNGIAPGYYYRM----------------------RDSGSFWNGSGCGNEFRSEAPMVRKFILDSVAYWVNEYKVDGFRFDLMGLIDLETMTQITETLHEIDPNILVYGEPWASGETPISITSKGKQRGLGFGVFNDNFRDALKGSPFDRFDISETFLIHGSRISEIKKGIEGSIHSFAQRPLESVNYMECHDNRTLWDQLHYLTQGQTD---IDARDIVQMVKLGAVIIFTSQGVPFIQMGQEMLRTKHDVENSYNAPDSVNQIEWERKIRYRDVVEYHKGLIQLRKAH-QMFRQTDPQAIEKNLKFLDNDLGMTVPDKCIAYQ-------------------------LEDERDEWERVLVLLNPNPISVIFPIPPGT----WTSVVDENQSGVEPIQIGIEREKRVAPRSAAVL 841          

BLAST of Gvermi6751.t1 vs. uniprot
Match: A0A3M2EUP4_9BACT (Pullulanase n=1 Tax=Candidatus Hydrogenedentes bacterium TaxID=2030809 RepID=A0A3M2EUP4_9BACT)

HSP 1 Score: 619 bits (1597), Expect = 1.140e-197
Identity = 381/939 (40.58%), Postives = 502/939 (53.46%), Query Frame = 0

Query:  487 IRYRRFSPSDYNGWDLWTWDPTDPSSHRVALAPVAETSGWA-DFIVDRACYGSAKD-ICLLPRRGGSSWLEKDGVERVWNASLFGNDNEVRVKSITSDFVSPGDTDYDDLPTFTITQGSSLVLRSLDDVMSMMEAFADSEH--SIVVRTPLPWGWICPPKKAGVASHPDVHFKLCKHPIHMSSNEGNQSFDHMAGRTLCHQKSKQLSPTSIRFFFDDKVAHFDEDFLVENVIVHAPPLEPACLRWETFQDEDRYFYEGSLGWTYSTRQCVFRCFAPASDQVSVVLYEKATGDAGRDVVPMRRIPQGCWKVIVAKDLKGMYYKLLAEGENKRLFPGVEVIDPYSRCNTAHTGRGLIYGTDMTPIYDRPKLRVADNIVYELHIRDVTIDKFSGIRNGGKFLGLTERGTKMMLKSGEHPHQTPLTPWEQEIMPGADKDFSILNKFSTGLDHIAQMGVTAIQIMPIQDFDNNEADESEYRWGYMPVHFNSPDGWYATSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGKRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKYWVTEFGVDGFRFDLLGLIDVKTVQLAVEELKIIDPNIVVYGEPWIGGLSPIKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDMFADSPHESINYVECHDNRTLWDHMKHYIRMRTDTIKFSEEDIKRMHKLAAVLVLTSQGIPLIQAGQEMCRTKFDDENSYESPDEINMIRWDAKLKEWPIVQYYRGLILLRRAHPEIFCMENAEEIHERIVFYED-CGLHVPDRCIAYRIKGNPEQLLTQLRKQNSRSGCEEEDLKEQSLQWSEVVVLFNATPVQVVFELPGKKADVMWMQTVSASVAGTRTISGP 1420
            + Y R    DY GW LWTW+P   +S  +  A    T G+   F +D+  YGS  D I LLP+ G   W  KD  +R W  SL  N            F+   D +    P  T  +    +L  +++V  ++    D  +     V   LP     P  +A  A  P    K+                     RT+  +  +++ P S+               +V        PL P  +        D Y Y+G LG  YS  + +FR F+P S  VSVV+YE  TG   RDVVPM+RI QG W+ IV  +L   YYK   +  +       EVIDPYS CNTAH GRG+I   D TP+   P+  +AD ++YE+HIRD TID  SGI++ GK+LGLTE GT++                  E  P          + STGLDHI ++GV  +QIMP QDFDN+EA E+ Y WGYMP HFNSPDGWYAT    A+RV+E KK++ A H+ G++V+MDVVYNHTAE     N E R SFNGLAP YYYR      V  +G                 Y NGSG GNEFR+E+PM RKFI+DS+KYWV ++ VDGFRFDL+GLID++T++  V EL+ I P I++YGEPW GG +P+K T KG QR KGFAVFND FR+A++G  F+TE  FI DG  + +VK GI+GS+D F D+P E+INYV  HDN TLWD +    R  T   K   +D ++M KLAAV +LT+QGIP +  GQ++ RTK  +ENSY  PD INMI W+ K K   + +YYRGLI LRRAHP +F    A+ +   + F +D   L VP R I + +               +R   ++E        W  V+VL NA+     FE+P       W+   +   AG   +  P
Sbjct:   79 VHYHR-PEGDYTGWTLWTWNPASGTSREITPAG---TDGYGLVFRLDKNDYGSNVDRIGLLPKYG--DWKAKDDPDRYWTPSLGRNV-----------FILSADKNLYSSPPDTRPRILRALLDKVNEVRVVLSKSIDRSYVRKGTVSVRLPDRSAVPVVEAVPA--PGQGLKV---------------------RTILVRLGQEIDPESVA------------GIVVSFGAYRPAPLIPGAVV-------DSYRYDGELGALYSPEKTIFRTFSPTSAGVSVVIYEGPTGPP-RDVVPMKRIGQGIWEAIVEGNLLDKYYKYRVKYPDAEY----EVIDPYSHCNTAHNGRGMIID-DNTPVSPAPRFGIADAVIYEIHIRDFTIDPKSGIQHRGKYLGLTEEGTRL------------------EGNP----------EISTGLDHIKELGVNTVQIMPFQDFDNDEASEA-YNWGYMPFHFNSPDGWYATKRDDASRVREAKKMIDAFHRNGIKVVMDVVYNHTAEG----NPEVRMSFNGLAPNYYYR------VKDDGS----------------YWNGSGTGNEFRTEAPMARKFIVDSMKYWVRKYDVDGFRFDLMGLIDLETLETIVRELRKIKPEIMIYGEPWAGGSTPVKVTSKGDQRGKGFAVFNDHFRDALKGGTFNTEPGFIQDGRDVEKVKKGILGSIDDFTDAPTETINYVAAHDNHTLWDRILLTTRNWTQVTK---KDREQMDKLAAVAILTAQGIPFLHGGQDLLRTKDGEENSYNKPDRINMIHWEWKKKHREVFEYYRGLIALRRAHP-LFRKTTADAVRRGVFFLDDDLELRVPPRAIGFVV---------------NRGEAQDE--------WENVLVLMNASATSARFEIPASH----WIPVANGEKAGVTPLGPP 866