Ggra5929.t1 (polypeptide) Gracilaria gracilis GNS1m male

BLAST of Ggra5929.t1 vs. uniprot
Match: A0A2V3J630 (Putative ubiquitin-conjugating enzyme E2 23 n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3J630_9FLOR)

HSP 1 Score: 947 bits (2449), Expect = 0.000e+0
Identity = 537/1133 (47.40%), Postives = 714/1133 (63.02%), Query Frame = 0

Query:    6 ELFDLVSLPNQALAVVIRLPYVDDSSSEESSDEGEWTWPTTTRRRH---LISRYEDTETVPNADTHVHETPYAPDDDSKIPIPDGFALVANVSTFNESLHKIEDLALRDRPLLRGNIVTNAANPSQSAVVVDVNRRLTVRRVSDISRTSSRRRATKNSFEVDQSQLGFFFGLCPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINADVRAGSLHKDEPLNEGFHYPGQTVTASSETWTHSAVWIRGSYTGIDVGTVTNVRMRQVEVHWIAQSNSAGETWEKDATSVELVDFSDITPLESFRQSCWIVGDKGFLVNPSSSTWKKGDLYHATVPFDNEAPRILNSDPDE-------SMRNESQFDEDSEEWIEEDDDDRQSTFSDDSHAPQGRFFKAQRRARRKESHAEYIETSEHMTPPEVVRPEDVVQVIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGRKEDQSMHGNQRSRKDARRGVIVRVNHLEKTAIVKSERPDAHQSDVWDTPEEEVSVYELTDDDYDYQIGDTVLHLASKSPNTLMDWVGIILDHQRGEATVKWVSGAVTKAKCSELVRLDTEIGDEEGSVSDSA-------YSGDDDDSDAVRYFEAEAGFTSPELRLNWGSDTDSDNSDYGMSDLDYGSYVVRSRLEGIFASNMFSTALTEEKMKKSIQLAVEYAVEAIEERKRINGSEGITLSRNEVALVAGILVTRAVSELLARSLIGPHEESREKLEPGSKDVEEKLSKFSAALVTAFECLISG----VVRQKQSQLENGTHENGNLDPSS-SAMEVDNHE------SHLSTDLPVMNNDRPLQVLENSSYNTVPKAALPKFCVLEELLDFFYDPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARCRSLIHGDVARATL-STSKSLSGNQKPYTSSGFKRSLEPLVHQLENAIHPL 1109
            E+FDLV +PN++L ++ RLP+ DD                         LIS YED E + +AD H+HE+P +P       +PDGFA V N   F+  LHK+ DL L DR L R   VTN + P Q+A V+DV+++LTVRR+S I+  +         FE+ QS LGFFFG+  GDTVV    +G V + SADVYV  SDGSLA +PSHD ++P++ AD    S    +P  EG  YPGQTV A  ETW+H+AVW+RG+Y  ID   VT+VRMR V V+W+AQ+ S+      D   ++LV +S++  L  FR+S W +GD+GFLVN  ++ WK G ++HAT+PF NE                  S R  S +  D   +         S  SD    P GRFF AQRRARRK ++AEYIET+E M P   V P DVVQ++ T+T+VTLRWQDGT E NVPA++LR ND+ D NDF PG++V RKED +++ +  +  D RRG IVRVN+ EKTA V+    D      WD   EEVSVYEL  DDYDY IGD VL+   KSPN LMDWVGII+D QRGEA VKW+ G  +   C++++ ++T   D                YS DD+DS AV++F AE G T  E+R N G   + D +   +SD+  G + + ++L+ + +       LT+  + +S+Q+A++ A+E ++ERKR+  S   TL+R+++A  A ILVTR VSEL  R+++    +  E  +  S  +  +L     +L+ AF  L+      +V  ++ Q        G +  SS S M+VD+ +      S+L  +   +  D+        S++     +L KF VL+E+ DF +  +    +R   FL V+SKEWRRLRTSLP GI+VR CE+H NI+RAAIIGSE TPYAD +FFFD+L+  RYPV PP VWF S+GRRLNPNLYEDGKVCLSILGTWDGDD+ESW  +TSNLLRVLLSLQA VFVEEPYYNEAG+G QRGT  G+NNS GYNENVFLLC+KHI+S+L++ Q P DCYE+A +HY+VAG KILARCR+L+ GDVA A + S SKSL  + K Y+S+GF++SLE L+  LENA++PL
Sbjct:    6 EIFDLVRMPNRSLGLISRLPHSDDXXXXXXXXXXXXXXXXXXXXXXXXXLISSYEDVENIHSADPHLHESPLSPSHPGSSGVPDGFAQVTN---FHPVLHKLPDLTLIDRALQRNLAVTNPSRPFQNATVIDVHKQLTVRRISSITNPTILPADAPELFELPQSHLGFFFGIRQGDTVVHSDRIGTVNSYSADVYVVFSDGSLAFVPSHDKQYPLVPADRLTPSYRPIDPNMEGIFYPGQTVKAPPETWSHAAVWLRGAYASIDTAVVTHVRMRDVLVNWVAQNPSSTSPPIHDEFQLDLVPYSELILLNEFRESSWCIGDRGFLVNSHNNRWKNGGVFHATIPFHNEXXXXXXXXXXXXXXXXXXSARTLSDWPADHHLYP--------SPSSDQHEMPAGRFFMAQRRARRKHTNAEYIETAEDMIPTSTVNPLDVVQIVATKTYVTLRWQDGTIEENVPAVDLRRNDYMDENDFTPGELVMRKEDAALNSHLDNGMDHRRGAIVRVNNAEKTATVQWVNTDPAIKSPWDPQMEEVSVYELVRDDYDYAIGDVVLNFNPKSPNLLMDWVGIIIDQQRGEANVKWLGGETSNVSCADIILVNTATNDXXXXXXXXXXXXXXXXYSEDDNDSGAVQFF-AEGGITLGEVRQNGGEVDEPDMN--ALSDIQLGRFSLLTQLQALMSFRKLKPKLTKASINESVQVALKNALEVVQERKRMRFSSD-TLTRDDIAASAAILVTRTVSELHTRAMLHGDSDHSEMHDEESGTLMLRLE-LQDSLLEAFNILMKSDGEPIVPAQRGQ--------GAVPISSMSVMDVDSQQGKPASPSNLQVETDCVEGDK--------SHDDGTNTSLQKFDVLDEIHDFHFTRSDEQPNRTQMFLFVVSKEWRRLRTSLPPGILVRVCEQHSNIMRAAIIGSEETPYADTIFFFDILMGVRYPVDPPSVWFWSYGRRLNPNLYEDGKVCLSILGTWDGDDIESWHLKTSNLLRVLLSLQALVFVEEPYYNEAGYGKQRGTPVGENNSRGYNENVFLLCVKHIISTLEKRQTPRDCYEIAQKHYRVAGPKILARCRALVRGDVAEARVCSASKSLE-DWKRYSSAGFRKSLEQLLLPLENALNPL 1105          

BLAST of Ggra5929.t1 vs. uniprot
Match: R7QRZ4 (UBC core domain-containing protein n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QRZ4_CHOCR)

HSP 1 Score: 549 bits (1414), Expect = 5.160e-173
Identity = 381/1082 (35.21%), Postives = 563/1082 (52.03%), Query Frame = 0

Query:   84 IPDGFALVANVSTFNESLHKIEDLALRDRP----LLRGNIVTNAANPSQSAVVVDVNRRLTVRRVSDISRTSSRRRATKNSFEVDQSQLGFFFGLCPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINADVRAGSLHKDEPLNEGFHYPGQTVTASSETWTHSAVWIRGSYTGIDVGTVTNVRMRQVEVHWIAQSNSAGETWE-KDATSVELVDFSDITPLESFRQSCWIVGDKGFLVNPSSSTWKKGDLYHATVPFDNEAPRILNSDPDESMRNESQFDEDSEEWIEEDDDDRQSTFSDD----------SHAPQ------------GRFFKAQRRARRKESHAEYIETSEHMTPPEV-VRPEDVVQVIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGRKEDQS-MHGNQRSRK------DARRGVIVRVNHLEKTAIVKSERPDAHQSDVWDTPEEEVSVYELTDDDYDYQIGDTVLHLASKSPNTLMDWVGIILDHQRGEATVKWVSGAVTKAKCSELVRLDTEIGDEEGSVSDSAYSGDDDD------SDAVRYFEAEAGFT---SPELRLNWGSDTDSDN---SDYGMSDLDYGSYVVRSRLEGIFASNMFSTALTEEKMKKSIQLAVEYAVEAIEERKRI-NGS-EGITLSRNEVALVAGILVTRAVSELLARSLIGPHEESREKLEPGSKDVEEKLSKFSAALVTAFECLISGVVRQKQSQLENG---THENGNLDPSSSAMEVDNHESHLSTDLPVMNNDRPLQV-LENSSYNTVPKAALPKFCVLEELLDFFYDPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARCRSLIHG-DVARATLSTSKSLSG--NQKPYTSSGFKRSLEPLVHQLENAIHPL 1109
            +P+G ALV   +T  + + K++DL L DR     LL   +        QS +V +V + + VRRV DI+  +        +FE+   +LGFF G+  GDTVV G +VGV+ +   DV+V  SDG++A +P H  R  ++N D R     + +P  EG +YPGQ V +    W   A WIRG Y+G D G V +V +  V + W+A+S  A  + E +    ++++ F+D+T LE FR   W VGD+G+L+ P  +  +  D      P D  A  ++ +                 E +EE DD     + +D          S +P             GR  KAQRRAR + +    + T    T      +PEDVVQV+GT+T   + WQDG+    VP  +LR     D  DF PG +V R ++++ + GN+ +        D ++G +V+VN  E+TA+VK ++       V    EEE+SVY+L   +Y+  +G+TVLH+  +S     +WVG+I     GE+TV W  G V+    +EL+ +    GD+E       YS +  D      SD +   EA   F    SP  + NWG+D D ++   SD+ +     G  V++  L        F       K ++S++       + ++ R+ + +GS     +S +E   +AG  V + + +LL +      E  R++        EE  +      V     +   +++++   L        E  ++  + S +  + +E+  + ++ +   D  + V  E  S        LP   V    +D  +D     ++ +  FL+V+ KEW RLR SLP G+IVRACE   +  RA I+G E TPYAD++FFFD+ L  +YPV PPHVWF SHGRRLNPNLY+DG+VCLSILGTWDGDD+ESW+ +TSNLLRVLLSLQA VFVEEPYYNEAG+G Q+GT  G  NS  YNE+  LL +++IL SL+    P DC ++A  HY+ AG K+L RCR LI   DV  A  S+    +G  +Q+PY S GF+RSL  L+  LE     L
Sbjct:  114 LPEGCALVVMAATGEKEVCKLDDLELLDRNFDPGLLAAWVEEGPDGLKQSGLVEEVRKTVIVRRVVDITPATVAVADDATTFELPAERLGFFLGIRAGDTVVSGSWVGVIDHFQEDVFVEFSDGAIACMPGH--RKALLNVDERTPDRARPDPFTEGLYYPGQKVRSIPPVWQAVATWIRGGYSGKDEGIVKSVSVGDVSIEWVAKSMYASHSDESRKEEGLQVMRFADVTLLEGFRSLLWSVGDRGYLLEPELADGRVADR---ATPVDG-ANGVIGT-----------------EVVEEADDGXXGDWEEDLVLDHSGAGPSSSPAMPPRKMRSRRHGGRLMKAQRRARERSAGGANVATVTDATVASASAKPEDVVQVVGTKTTADIVWQDGSRSKGVPTTSLRLLHHSDPYDFWPGTVVLRTDEEADVPGNEGTEVKKPSLFDIKKGAVVKVNQNERTALVKWQKDSESAFIV----EEEMSVYDLKIAEYEVGVGETVLHVPQQSDEQRNEWVGVITKQAMGESTVAWTGGKVSAVPTTELLYITG--GDDESFEGMEHYSLEGSDQPSPGSSDEIPSREAPPHFQVQDSPVYQQNWGTDDDGEDGGDSDHVVFSFLAGKEVIKRHLSSA-TIRAFHRGADWVKFERSVENIGTDVSDRLKVRRFMPDGSLRRREISTDEERRLAGAFVFKVLGDLLLQ-----FERERDEDVDAEVRSEEAANDEWTDFVNLVVLIFGRIMKKETEWLSKQLAMVGEKVDVKLADSTLHGEKNEAD-AREMEIT--DEQVNVPSEADSGGPASFEVLPDLNVFHHFMDVPWD-----ATYSKGFLTVVHKEWNRLRKSLPPGMIVRACESTVDRFRAGIVGPEDTPYADVIFFFDIRLGPKYPVMPPHVWFHSHGRRLNPNLYDDGRVCLSILGTWDGDDIESWNAKTSNLLRVLLSLQALVFVEEPYYNEAGYGKQKGTKEGHTNSRMYNESALLLSMRYILQSLKVGNLPEDCRDIAISHYRKAGPKLLERCRQLIQAKDVKPAGPSSDTGDAGTSDQQPYPSVGFQRSLVQLLGSLEGVFSSL 1152          

BLAST of Ggra5929.t1 vs. uniprot
Match: probable ubiquitin-conjugating enzyme E2 23 n=1 Tax=Mercenaria mercenaria TaxID=6596 RepID=UPI001E1DEAE4 (probable ubiquitin-conjugating enzyme E2 23 n=1 Tax=Mercenaria mercenaria TaxID=6596 RepID=UPI001E1DEAE4)

HSP 1 Score: 400 bits (1029), Expect = 4.730e-117
Identity = 368/1143 (32.20%), Postives = 531/1143 (46.46%), Query Frame = 0

Query:   84 IPDGFALVANVSTFNESLHKIEDLALRDRPLLRGNIV---------------------TNAANPS----QSAVVVDVNRRLTVRRVSDISRTSSRRR----ATKNSFEVDQSQLGFFFGLCPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINADVRAGSLHKDEPLNEGFHYPGQTVTASSETWTHSAVWIRGSYTGIDVGTVTNVRMRQVEVHWIAQSNSAGETWEKDATSVELVDFSDITPLESFRQSCWIVGDKGFLVNPSSSTWKKGDLYHATVPFDNEAPRILNSDPDESMRNESQFDEDSEEWIEEDDDDRQSTFSDDSHAPQGRFFKAQRRARRKESHAEYIETSEHMTPPEVV-----------RPEDVVQVIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGR-------------KEDQSMHGNQRSRKDARR-GVIVRVNHLEKTAIVKSERPDAHQSDVWDTPEEEVSVYELTDDD-YDYQIGDTVLHLASK--------SPNTLMDWVGIILDHQRGEATVKWVSGA--VTKAKCSELVRLDTEIGDEEGSVSDSAYSGDD---DDSDAVRY--FEAEAGFTSPE-----------LRLNWGSDTDSDNSDYGMSD------LDYGSYVVRSRLEGI----FASNMFSTALTEEKMKKSIQLAVEYAVEAIEERKRINGSEGITLSRNEVALVAGILVTRAVSELLARSLIGPH---EESREKLEPGSKDVEEKLSKFSAALVTAFECLISGVVRQKQSQLENGTHENGNLDPSSSAMEVDNHESHLSTDLPVMNNDRPLQVLENSSYNTVPKAAL----PKFCVLEELLDFFY----DPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARCRSLIHGDVA----RATLSTSKSLSGNQ-----KPYTSSGFKRSLEPLVHQLENAIHPLAGSNPS 1115
            +P G A V  V + ++ +  +E L + DR  + G I+                     T+ + P+    Q+A +V V + L+VRRVS  S   SRR     A + +F++   +LGF  GL  G  VV  +++G VT  +  V V  SDGS+     H     ++ AD+   +L  D    EG +YPGQ V A  E W  SA+W+ GSY+   VGTV +VR+  V V W+A   +     E ++   ++V  +D+TPL +FR   W VGD+G              LY   VP D  AP    +DP         +DE+  EW E  + D +   + D   P  R      R RR  S      TS  +                 + E VV+V+ T+T++ L WQDG+   +VPA+ +  N      D+ PG  V               +E++   G+ +S    +R GVIVRVNH  +TA+V+ ++ D  +      P+E+VSVYEL++   YD ++GDTVL +  +        +P    DWVG ++    GE  V W  G        C  +                    GDD   D +D + +  F  + G  S             L  NW  + D+ ++  GMS       +D  S V  +R   +    F  +  + A   E + ++  L     V +        G+  +TL       +A   V  AV      S   P     E++ K E  ++     + +  A   T     +SG ++ K +        +G+L   S+ +      S  +  L V ++  P+    NS   TV + +L     +FCV++EL    +      +PP +     F +VI KEW RL   LP GI V A E   ++LRA+IIG   TPYADI+FFFD+LL   YP  PP V F SHGRRLNPNLYEDGKVCLSILGTWDG+ VE WDPR SN+LRVLLSLQA VFVEEPY+NEAG+  Q GT+ G  N+  YNE+  LL ++H +SSL+    P D  E+   HY+  G  I+ RCR+LI   +     R     +  +  N+        TS GFKRSL  L+ +++ A   L     S
Sbjct:  139 VPVGSARVLLVESGHDVVLPLEKLMVEDRAFIPGEIICQKLSSHQQAVGAGTPDVALSTDGSPPANDQTQAATIVAVTKTLSVRRVSPQSSPPSRRHGSRAAEEPAFDIRSDRLGFISGLRDGCLVVLNNWMGRVTFFNELVDVRFSDGSICQCQGHPSE--LLPADIEDDTLLDDV---EGVYYPGQYVKAEPEVW-RSAIWLSGSYSRESVGTVVSVRVIDVGVDWMATRYTEEIGTEDESPPPDIVPAADLTPLGAFRDLWWRVGDRG--------------LYSGDVPSDRVAPSAT-ADP---------YDEEDGEW-EAVEGDNECPGNPDG--PHSRSRAGISRRRRSASRMAAGRTSNSLRRASRTSNSGVGSVTQNQNEHVVEVVNTKTYLDLLWQDGSRTYHVPAVTVLRNHHLGAYDYFPGIFVSDAQDDLEVDLSNQPEEEKDQLGHLKSSNRLKRIGVIVRVNHEARTAVVRWQQGDKCEYG----PDEDVSVYELSEHGVYDIRLGDTVLRVPPQGKSASPRGNPAFRRDWVGEVVGIGIGELEVSWHQGIREYVTPDCLLVXXXXXXXXXXXXXXXXXLSDGDDAPRDGNDQLTFSMFPHQNGNISDSRHNQGESRSINLPENWAEEEDN-SAPVGMSIPPVPSVVDEDSAVHAARFVSLALDPFRESFPTVAADPELVARAALLDAFIEVHS--------GTGALTLEN-----LANAGVKHAVRFSSGASRGSPSFSFGEAQAKPEYWTEVERVIVDRILAVFKT-----LSGPMQTKSTV-------SGSLSEDSARVGDAGLSSIAANSLLVSDDAAPV----NSC--TVDEISLHEDVERFCVIDELAHHHFGSGSSADPPAAVG---FPNVIRKEWNRLTRHLPAGIFVHASESRLDLLRASIIGPADTPYADILFFFDILLPTDYPSMPPKVQFMSHGRRLNPNLYEDGKVCLSILGTWDGEGVEKWDPRNSNVLRVLLSLQAMVFVEEPYFNEAGYEKQVGTAQGLANARLYNESTLLLSLRHAVSSLRPGGVPQDFSELTRLHYRQVGPAIVRRCRALIEEGITPVPTRENADEAMRIDPNRGQALRADNTSIGFKRSLANLLPKVQAAFDELLSDQGS 1209          

BLAST of Ggra5929.t1 vs. uniprot
Match: A0A251L1J4 (UBC core domain-containing protein n=1 Tax=Manihot esculenta TaxID=3983 RepID=A0A251L1J4_MANES)

HSP 1 Score: 281 bits (720), Expect = 3.030e-75
Identity = 296/1060 (27.92%), Postives = 471/1060 (44.43%), Query Frame = 0

Query:   99 ESLHKIEDLALRDRPLLRGNIVTNAANPS-QSAVVVDVNRR--LTVRRVSDISRTSSRRRATKNSFEVDQSQLGFFFGLCPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINADVRAGSLHKDEPLNEGFHYPGQTVTASSETWTHSAVWIRGSYTGIDV-GTVTNVRMRQVEVHWIAQSNSAGETWEKDATSVELVDFSDITPLESFRQSCWIVGDKGFL---VNPSSSTWKKGDLYHATVPFDNEAPRILNSDPDESMRNESQFDE--DSEEWIEEDDDDRQSTFSDDSH-APQGRFFKAQRRARRKESH----AEYIETSEHMTPP--------EVVRPEDVVQ-----------VIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGRKEDQSMHGNQRSRKDARRGVIVRVNHLEKTAIVKSERPDAHQSDVWDTPEEE-VSVYELTDD-DYDYQIGDTVLHLASKSPNTLMDWVGIILDHQRGEATVKWVSGAVTKAKCSELVRLDTE-IGDEEGSVSDSAYSGD----DDDSDAVRYFEAEAGFTSPELRLNWGSDTDSDNSDYGMSDLDYGSYVVRSRLEGIFASNMFSTALTEEKMKKSIQLAVEYAVEAIEERKRINGSEG--ITLSRNEVALVAGILVTRAVSELLARSLIGPHEESREKLEPGSKDVEEKLSKFSAALVTAFECLISGVVRQKQSQLENGTHENGNLDPSSSAMEVDNHESHLSTDLPV-MNNDRPLQVLENSSYNT-VPKA------ALPKFCVLEELLDFFYDPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARCRSLIHGDVARATLSTSKSLSGNQKP-YTSSGFKRSLEPLVHQLENAIH 1107
            E +  + D+ + DR  L G+ V +A++P+ Q  VV+DVN    L V   S I   SSR       F +             GD VV G ++G V ++  +V V + DGS+  +   +   P+    V       DE +    +YPGQ V ASS +   ++ +  GS+    + GTVT VR   V ++WIA   SAG   +      E     ++  L  F  + W VGD   L   +  SSS           +  D    ++   D  +S  +  Q +   DS++   E+ D    T   D   AP G   K       + S     A   +   H T P         V+R E   +           V+ TRT V + WQDG+ E  + +  L   D    ++F   Q V  K    +     +R   R GV+  VN  E+TA V+  +P     D  +  +EE VSVYEL    DYDY  GD V+ L+  S +      G  +   + +AT+   +  V   K S   + D E IG+     SD ++ G+     +    V + +       P+     G D D D+   G                    S +   A + E +      AVE   E +  +   +G+    +TL  N       + +  A  + + R   G     R+ ++P         S+    L T     +  +  ++ S  +  + ++  +D     +  DN E H + D+ V +++D P  +   S+  +  P        +  +F + ++ +D ++  +    +   ++L  + ++W  L+ +LPDGI VR  E   ++LRA I+G+ GTPY D +FFFD  L   YP  PP  ++ S G R+NPNLYE+GKVCLS+L TW G   E WDP +S++L+VL+SLQ  V   +PY+NEAG+  Q GT+ G+ NS+ YNEN FLL  K ++  +++   P D   +A +H++  G  IL  C + + G +   +L+   S+S N+    TS GFK  L  +V +L +A++
Sbjct:  152 EPIQHVNDIQVVDRGFLHGDYVASASDPTGQVGVVLDVNISVDLQVPDGSIIKDVSSRELKRVREFAI-------------GDYVVLGPWLGRVDDVLDNVTVLIDDGSMCKVVGAE---PLRLKPVSKSMFDVDEHIP---YYPGQRVRASSSSVFRNSWFQSGSWKANRLEGTVTKVRAGSVFIYWIA---SAGHGPDSSTAPAEEQSPKNLKLLSCFAHASWQVGDWCHLPPAIRQSSS-----------ITLDQGLSKLGLYDSVKSELSSDQLENGCDSDQVAPEESDGNNETMDTDPMVAPDGINGKTVSNGSSESSSCGSSASVSKDPVHETWPLHRKKIRKVVIRREKKARKKEENFEKALLVVNTRTRVDVAWQDGSIERGLESTLLIPIDSPGDHEFVAEQYVVEKSSDDIDNASEAR---RVGVVKSVNAKERTACVRWLKPVTRAEDPREFDKEEIVSVYELEGHPDYDYSYGDVVVRLSPVSVSAQATSDGESVRELKWKATLN--NSTVNVKKRSGSKKEDDEFIGEACLDFSDLSWVGNITGLRNGDIEVTWADGMVSTVGPQAIFVVGRDDDDDSIAAG--------------------SEVSDDAASWETVDDDEMDAVENGQEEVRLQNAAHGNSEAEVTLESNNSGRNPALSLPLAALDFVTRLATGFFSRGRKNVDPDFSG-----SRGENGLQTQG---VMDISEERDSADDTSSLKSNVIDNGGGQIIHDNVEEHSTIDMEVPISSDAPDTLCNLSTDKSDTPTCFEDDTLSFKRFDIAKDPVDHYFLGSNAQINNGRKWLKKVQQDWNILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPTSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLSCKTMMYLMRKP--PKDFEGLAQEHFRKRGYYILKACDAYMKGSLI-GSLTKDASISNNENSNLTSVGFKLMLAKIVPKLYSALN 1142          

BLAST of Ggra5929.t1 vs. uniprot
Match: A0A6J0P703 (probable ubiquitin-conjugating enzyme E2 23 n=1 Tax=Raphanus sativus TaxID=3726 RepID=A0A6J0P703_RAPSA)

HSP 1 Score: 279 bits (714), Expect = 1.180e-74
Identity = 270/954 (28.30%), Postives = 424/954 (44.44%), Query Frame = 0

Query:  175 CPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINADVRAGSLHKDEPLNEGFHYPGQTVTASSETWTHSAVWIRGSYTGIDV-GTVTNVRMRQVEVHWIAQSNSAGETWEKDATSVELVDFSDITPLESFRQSCWIVGDKGFLVNPSSSTWKKGDLYHATVPFDNEAPRILNSDP-----DESMRNESQFDEDSEEWIEEDDDDRQSTFSDDSHAPQGRFFKAQRRARRKESHAEYIETSEHMTPPEVVRPEDVVQVIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGRKEDQSMHGNQRSRKDARRGVIVRVNHLEKTAIVKSERPDAHQSDVWDTPEEEV-SVYEL-TDDDYDYQIGDTVLHLASKSPNTLMDWVGIILDHQRGEATVKWVSGAVTKAKCSELVRLDTEIGDEEGSVSDSAYSGDDDDSDAVRYFEAEAGFTSPELRLNWGSDTDSDNSDYGMSDLDYGSYVV-RSRLEGIFASNMFSTALTEEKMKKSIQLAVEYAVEAIEERKRIN-GSEGITLSRNEVALVAGILVTRAVSELLARSLIGPHEESREKLEPGSKDVEEKLSKFSAALVTAFECLISGVVRQKQSQLENGTHENG----NLDPSSSAMEVDNHESHLSTDLPVMNNDRPLQVLENSSYNTVPK----AALPKFCVLEELLDFFYDPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARC----RSLIHGDVARATLSTSKSLSGNQKPYTSSGFKRSLEPLVHQLENAI 1106
            C GD VV G ++G + ++  +V V   DGS+  +   +   P+    V   +L +D   N  +H PGQ V A+S +   S+ W  G +    + GTVT V    + V+WIA   SAG   +   +  E    S++T L SF  + W VGD G L + + S         AT+P      ++   D      D   ++ S  ++  +E  E+ D     T S +S  P    +   R+  RK      +   +     +    E  + ++ +RT V + WQDGT E    A  L   +    ++F P Q V  K   +   ++ + +  R GV+  VN  E+TA VK  +P     +  +  +EE+ SVYEL    DYDY  GD V+ L+          V ++L       +V+  +      K       DTE   +E  V D   +  D D   + +     G    ++ + W    D   S  G   +    YVV R   E + A +  S A + E +      A E   E +     I   S+      N+      + +  A  E + R   G     ++  +  S  + ++                      KQ++L N +HE G    N D   +A+E          +  ++ N+   +     S   VP      +  +F + ++ LD  Y  +    ++  ++   + ++W+ L+ +LPDGI VR  E   ++LRA I G+ GTPY D +FFFD  L   YP  PP   + S G RLNPNLYE+GKVCLS+L TW G   E WDP++S++L+VL+SLQ  V   +PY+NEAG+  Q GT+ G+ NS+GYNEN FLL  K ++  ++R   P D  E+  +H++  G  IL  C    R  + G + +      +S S N     S GFK  L  +  +L +A+
Sbjct:  221 CVGDYVVHGPWLGRIDDVLDNVTVLFDDGSMCRVLRAE---PLQLKPVSQSNLEED--ANFPYH-PGQRVKATSSSVFKSSRWFSGLWKPSRLEGTVTKVTAGSIFVYWIA---SAGFGPDSAVSPPEEQSPSNLTLLSSFTHANWQVGDWGLLPSVNQS---------ATIPLHKHVSKLRLYDSQAKCADREQKSGSDLEDVQDEVSEKADPVGVLTLSSNSKEPAHEPWPLHRKKIRK-----LVIRKDKRVKKKEESFEQALLIVSSRTLVDVAWQDGTVERRREATTLIPIETPGDHEFVPEQYVVEK---ASDDDENTTEPKRVGVVKSVNAKERTASVKWLKPRLMAEEPREFDKEEIASVYELEVHPDYDYCYGDIVVRLSP---------VAMVLPASSAGNSVEEETEKDNGVK-------DTEYR-QEAIVHDKEENEVDTDLSELSWVGNITGLMDGDIEVTWA---DGMVSTVGPQAI----YVVGRDDDESVGAESEASDAASWETIDDDKGGAPETHEEDLGRGSFIEESSDAENNGENDSGRNGALSLPLAAVEFVTRMASGIFSRGQKTEDSSSSSLTDENG-------------------YKQAELTNSSHERGSFLDNCDSEGTALE----------NSTILGNEAVKRSESEKSEEPVPSESDSCSFRRFDISQDPLDHHYLGSDDQKTKQRQWFKKVDQDWKILQNNLPDGIFVRVYEDRMDLLRAVITGAYGTPYQDGLFFFDFHLPPEYPTVPPSAHYHSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLGYNENAFLLNCKTMMYLMRRP--PKDFEELINEHFRKRGYYILKACDAYMRGYLIGSLTKDVSVIDESSSANSN---SVGFKLMLAKIAPKLFSAL 1090          

BLAST of Ggra5929.t1 vs. uniprot
Match: A0A0A0LLP6 (UBC core domain-containing protein n=12 Tax=Cucurbitaceae TaxID=3650 RepID=A0A0A0LLP6_CUCSA)

HSP 1 Score: 279 bits (714), Expect = 1.650e-74
Identity = 298/1063 (28.03%), Postives = 476/1063 (44.78%), Query Frame = 0

Query:   83 PIPDGFALVANVSTFNESLHKIEDLALRDRPLLRGNIVTNAANPS-QSAVVVDVNRR--LTVRRVSDISRTSSRRRATKNSFEVDQSQLGFFFGLCPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINADVRAGSLHKDEPLNEGF-HYPGQTVTASSETWTHSAVWIRGSYTGIDV-GTVTNVRMRQVEVHWIAQSNSAGETWEKDATSVELVDFSDITPLESFRQSCWIVGDKGFLVNPSSSTWKKGDLYHATVPFDNE---APRILNSDPDESMRNESQFDEDSEEWIEEDDDDRQSTFSDDSHAPQGRFFKAQRRARRKESHAEYIETSEHMTPP--------EVVRP-----------EDVVQVIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGRKEDQSMHGNQRSRKDARRGVIVRVNHLEKTAIVKSERPDAHQSDVWDTPEEE-VSVYELTDD-DYDYQIGDTVLHLASKSPNTLMDWVGIILDHQRGEATVKWVSGAVTKAKCSELVRL-DTEIGDEEGSVSDSAYSGD----DDDSDAVRYFEAEAGFTSPELRLNWGSDTDSDNSDYGMSDLDYGSYVVRSRLEGIFASNMFSTALTEEKMKKSIQLAVEYAVEAIEERKRINGSEGITLSRNEVALVAGILVTRAVSELLARSLIGPHE---ESREKLEPGSKDVEEKLSKFSAALVTAFECLISGVVRQKQSQLENGTHENGNLDPSSSAMEVDNHESHLSTDLPVMNNDRPLQVLENSSYNTVPKAALPKFCVLEELLDFFYDPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARCRSLIHGDVARATLSTSKSLSGNQKPY-TSSGFKRSLEPLVHQLENAIH 1107
            P+PD    V  +   +E+   + DL + DR  + G+ V   ++P+ Q+ VVVDVN    L V   S +   SS+       F V             GD VV G ++G V ++  +V V   DGS   +   +         +R   + K+   +  F +YPGQ V A+S  + +S  W+ G +    + GTVT V +  V ++WIA   SAG   +      E     ++  L  F  + W +GD   L+ PS S     D     +   N    A  +   D ++++ +E                  +ST  D   A  G +      +  + S +  ++ + H T P         VVR            E  + +I T+T V + WQDG TE+ + + +L   D    ++F P Q V  K   +      SR   R GV+  V+  E+TA V+  +P +   D  +  +EE VSVYEL    DYDY  GD V+ L+  S +     +GI  +  + +++   +         S   ++ DT   D+    SD ++ G+     +    V +         P+     G D D ++   G S++  G+    +  E +    M S     E   + I+L    A    EE ++ N    + LS   V L A   VTR  + + +R    P     +S  + E  S D++    K S    T+ +   S          + G  E+G     S  +E     S+L T             L+ S+ +     +   F + ++ LD ++      ++   ++L  I ++W  L+ +LPDGI VR  E   ++LRA I+G+ GTPY D +FFFD  L   YP  PP  ++ S G R+NPNLYE+GKVCLS+L TW G   E WDP++S++L+VL+SLQ  V   +PY+NEAG+  Q GT+ G+ NS+ YNEN FLL  K I+  +++   P D  E+  +H++  G  IL  C + + G +   +L+   S+     P  TS GFK  L  +V +L ++++
Sbjct:  138 PLPDNEVRVLWMDE-SETTQHVNDLTVIDRGFVHGDFVAAVSDPTGQAGVVVDVNISVDLLVPDGSIMKDISSKDLKRVRDFTV-------------GDYVVLGPWLGRVDDVLDNVTVMFDDGSKCKVTKAEP--------LRLKPVSKNTLEDANFPYYPGQRVRATSTVFKNSK-WLSGLWKPNRLEGTVTKVTVGSVFIYWIA---SAGYGPDSSTAPAEEQTPKNLRLLTCFSHANWQLGD-WCLLPPSFSAGLTKDPSQTELSVTNTLDCAQSVGACDSEDTVLDELSGT-------------TESTDLDSISACDGNYRNPVDNSLPESSSSRALKETAHETWPLHRKKIRKVVVRRDKKARKKEENFERALLIINTKTRVDVAWQDGQTELGLDSTSLIPIDNPGDHEFVPEQYVVEKASDNDDDVSESR---RVGVVKSVHAKERTACVRWLKPVSRAEDPREFDKEEIVSVYELEGHPDYDYCYGDVVVRLSPVSDSAEAMSLGINTEELKQQSSTNEMMSCTEFNNASGSQKIEDTSCSDDCIDFSDLSWVGNITGLKNGDIEVTWANGMVSTVGPQAIYVVGRDDDDESIAAG-SEVSNGA----ASWETVDNDEMDSV----ENAAEDIELQDTGANSEEEESEQSNSGRNLALS---VPLAALRFVTRLAAGIFSRGPRNPDSMDLDSHSESEIQSLDIQASEGKDSGLQSTSLK---SNSFDASDMNSDCGRGEDGVASEPSEVLESAKTSSNLRT-----------VELDASACHEDGTCSFKGFDIAKDPLDHYFLGTNGQTNNGRKWLKKIQQDWSILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFFFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLSYNENTFLLNCKTIMYLMRKP--PKDFEELIKEHFRRRGYFILKACDAYMKGHLI-GSLTEDASVRVESDPNSTSVGFKLMLAKIVPKLFSSLN 1128          

BLAST of Ggra5929.t1 vs. uniprot
Match: A0A2P2JDJ2 (Uncharacterized protein MANES_04G071900 n=2 Tax=Rhizophora mucronata TaxID=61149 RepID=A0A2P2JDJ2_RHIMU)

HSP 1 Score: 278 bits (711), Expect = 3.650e-74
Identity = 294/1059 (27.76%), Postives = 467/1059 (44.10%), Query Frame = 0

Query:   99 ESLHKIEDLALRDRPLLRGNIVTNAANPS-QSAVVVDVNRR--LTVRRVSDISRTSSRRRATKNSFEVDQSQLGFFFGLCPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINADVRAGSLHKDEPLNEGFHYPGQTVTASSETWTHSAVWIRGSYTGIDV-GTVTNVRMRQVEVHWIAQSNSAGETWEKDATSVELVDFSDITPLESFRQSCWIVGDKGFLVNPSSSTWKKGDLYHATVPFDNEAPRILNSDPDESMRNESQFDE-----DSEEWIEED-DDDRQSTFSDDSHAPQ-------GRFFKAQRRARRKESHAEYIETSEHMTPPEVVRP-----------EDVVQVIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGRKEDQSMHGNQRSRKDARRGVIVRVNHLEKTAIVKSERPDAHQSDVWDTPEEE-VSVYELTDD-DYDYQIGDTVLHLASKSPNTLMDWVGIILDHQRGEATVKWVSGAVTKAKCSELVRLDTEIGDEEGSVSDSAYSGDDDDSDAVRYFEAEAGFTSPELRLNWGS-----------------DTDSDNSDYGMSDLDYGSYVVRSRLEGIFASNMFSTALTEEKMKKSIQLAVEYAVEAIEERKRINGSEGITLSRN---EVALVAGILVTRAVSELLARSLIGPHEESREKLEPGSKDVEEKLSKFSAALVTAFECLISGVVRQKQSQLENGTHENGNLDPSSSAMEVDNHESHLSTDLPVMNNDRPLQVLENSSYNTVPKAALPKFCVLEELLDFFYDPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARCRSLIHGDVARATLSTSKSLSG-NQKPYTSSGFKRSLEPLVHQLENAI 1106
            E +  +  +A+ DR  L G+ V +A++P+ Q  VVVDVN    L     S +   SSR       F V             GD VV G ++G V ++  +V V + DGS+  +   +   P+    +     ++DE      +YPGQ V A S +   ++ W+ G +    + GTVT V    V ++WIA   SAG   E      E     ++ PL  F  + W VGD   L+ P        D+  ++V  D    ++   D  +++RN+ +  +     D EE   ED D +  S   +   AP        G    A  ++  KE H +            V+R            E  + ++ TRT V + WQDGTTE  + +  L   D    ++F   Q V  K    +  N  +R   R GV+  VN  E+TA V+  +P +   D+ +  +EE VSVYEL    DYDY  GD V+ L   S ++ M              T    SGA  K +  E    + + G       D+++     D   + +     G  + ++ + W                   D DS  +   +SD D  S+      E +   +M +   T+E      ++ +  A +   E +   G E     RN    + L A   VTR  + + +R      +    K   G  +++ ++S                +  +K S  E+ +  +  +D S         E     D PV ++       ++  +N        +F ++++ LD  +  +   ++   ++   + +EW  L+ +LP+GI VRA E   ++LRA I+G+ GTPY D +FFFD  L   YP  P   ++ S G R+NPNLYE+GKVCLS+L TW G   E WDP +S++L+VL+SLQ  V   +PY+NEAG+  Q GT+ G+ NS+ YNEN FLL  K ++  +++   P D  E+  +H++  G  IL  C + + G +   TL+   S+S  N    TS GFK  L  +V +L  A+
Sbjct:  153 EPIQYVNSIAVVDRGFLHGDYVASASDPTGQVGVVVDVNISVDLLAHDGSIVKDVSSRDLKRVREFAV-------------GDYVVHGAWLGRVDDVLDNVTVLVDDGSVCKVMGAE---PLRLKPISKNMFNEDEHFP---YYPGQRVRAVSSSVFRNSRWLSGLWKHNRLEGTVTKVTTGSVFIYWIA---SAGYGPESSTAPPEEQSPKNLKPLSCFAHANWQVGD-WCLLPP--------DIKASSVILDRGLSKL---DLHDAIRNDLESSQPESGCDLEEVTPEDLDQNNVSMDIEPETAPYTATDCLPGSSSCASSKSVSKEGHHDIWPLHRKKIRKVVLRRDKKARKKEETFERALMIVNTRTKVDVVWQDGTTEHGLDSTKLIPIDSPGDHEFVAEQYVVEKASDDVDNNSEAR---RVGVVKGVNAKERTACVRWLKPVSRAEDLREFDKEEIVSVYELEGHPDYDYSYGDVVVRLTPVSISSKM--------------TSDGESGAKPKQQTEEDDIANAKKGTGFKKAGDTSWDEASIDFSDLSWVGNITGLKNGDIEVTWADGMVSMVGPQAIFVVGRDDDDSAAAGSEVSD-DAASW------ETVSGDDMSALENTQE------EVGLLNATDTNSEEE--GGLESDNSGRNPALSLPLAALDFVTRLATGIFSRGQKNDPDFVESK---GENELKGRIS----------------ISEEKDSVDESSSQRSNIIDSSDLQSVYFKEEKDADLDAPVSSDAAACSANDSYLFN--------RFDLVQDPLDHHFCGSNVQANNGRKWAKKVQQEWNILQNNLPEGIYVRAYEDRMDLLRAVIVGAYGTPYQDGLFFFDFQLPPEYPDVPLSAYYHSGGWRVNPNLYEEGKVCLSLLNTWTGRGNEVWDPTSSSILQVLVSLQGLVLNSKPYFNEAGYDKQIGTAEGEKNSLSYNENTFLLNCKTMMYLMRKP--PKDFEELINEHFRRRGYYILKACNAYMKGSLI-GTLTKDASMSNKNNSNLTSVGFKLMLAKIVPKLYLAL 1115          

BLAST of Ggra5929.t1 vs. uniprot
Match: A0A067KBQ3 (UBC core domain-containing protein n=1 Tax=Jatropha curcas TaxID=180498 RepID=A0A067KBQ3_JATCU)

HSP 1 Score: 278 bits (711), Expect = 3.710e-74
Identity = 299/1072 (27.89%), Postives = 463/1072 (43.19%), Query Frame = 0

Query:   99 ESLHKIEDLALRDRPLLRGNIVTNAANPS-QSAVVVDVNRR--LTVRRVSDISRTSSRRRATKNSFEVDQSQLGFFFGLCPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINADVRAGSLHKDEPLNEGFHYPGQTVTASSETWTHSAVWIRGSYTGIDV-GTVTNVRMRQVEVHWIAQSNSAGETWEKDATSVELVDFSDITPLESFRQSCWIVGDKGFL---VNPSSS-TWKKGDL---YHATVPFDNEAPRILNSDPDESMRNESQFDEDSEEWIEEDDDDRQSTFSDDSHAPQGRFFKAQRRARRKESHAEYIETSEHMTPPEVVRP------------------------EDVVQVIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGRKEDQSMHGNQRSRKDARRGVIVRVNHLEKTAIVKSERPDAHQSDVWDTPEEE-VSVYELTDD-DYDYQIGDTVLHLASKSPNTLMDWVGIILDHQRGEATVKWVSGAVTKAKCSELVRLDTEIGDEEGSV--SDSAYSGDDDDSDAVRYFEAEAGFTSPELRLNWGSDTDSDNSDY-----GMSDLDYGSYVVRSRLEGIFASNMFSTALTEEKMKKSIQLAV------EYAVEAIEERKRINGSEGITLSRNEVALVAGILVTRAVSELLARSLIGPH---EESREKLEPGSKDV----EEKLSKFSAALVTAFECLISGVVRQKQSQLENGTHENGNLDPSSSAMEVDNHESHLSTDLPVMNNDRPLQVLENSSYNTVPKA------ALPKFCVLEELLDFFYDPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARCRSLIHGDVARATLSTSKSLSGNQKPYTSSGFKRSLEPLVHQLENAIH 1107
            E +  + D+ + DR  L G+ V +A++P+ Q  VV+DVN    L V   S I   SSR       F +             GD VVRG ++G V ++  +V V + DGS+  +   +   P+    +      +DE      +YPGQ V ASS +   +  W+ GS+    + GTV  V    V ++WIA   SAG   +   T +E     D+  L  F  + W VGD   L   +  SSS T  KG      H +   +++A ++ N    E +  E            E D++ +S   D +  P G   K        ES      TS    P     P                        E  V ++ TRT V + WQDGT    + +  L   D    ++F   Q V  K   +   N  + +  R GV+  VN  E+TA V+  +P A   D  +  +EE VSVYEL    DYDY  GD V+ L+  + +T             GE   K         K     + + ++   E  V  SD  + G+              G  + ++ + W     S          G  D    S    S +    AS  + T   +E     IQ  V      +   EA E  +  N      LS   + L A   VTR  + + +R+    H    ESR + E  S+ +    EE+ S   +               QK + ++NG   + + D    A+        L T++PV +N        ++  + +P        +  +F + ++ LD ++  +   +++  ++L  + ++W  L+ +LPDGI VR  E   ++LRA I+G+ GTPY D +F FD  L   YP  PP  ++ S G R+NPNLYE+GKVCLS+L TW G   E WDP +S++L+VL+SLQ  V   +PY+NEAG+  Q G++ G+ NS+ YNEN FLL  K ++  ++R   P D  E+  +H++  G  IL  C + + G +  +    +   + +    TS GFK  L  +V +L  A++
Sbjct:  131 EQIQHVNDIKVVDRGFLHGDYVASASDPTGQVGVVLDVNISVDLLVPGGSIIKDVSSRVLKRVREFAI-------------GDYVVRGPWLGRVDDVLDNVTVLIDDGSVCKVNGAE---PLRLKPISKSIFDEDEHTP---YYPGQRVRASSSSVLRNVRWLSGSWKANRLEGTVAKVTAGSVFIYWIA---SAGYGPDSSTTPIEEQSPKDLKLLSCFSHANWQVGDWCLLPPAIRQSSSITLHKGLSKLGLHDSSKSESDASQVGNGCDSEEVAPE------------ESDENNESMDIDPAVVPDGNNGKNIGSNSSLESSFFVSSTSVSKDPVHETWPLHRKKIRKVVIRREKKVRKKEENFERAVLIVDTRTRVDVAWQDGTIGRGMESTTLIPIDSPGDHEFVAEQYVVEK---ASDDNDNASEARRVGVVKSVNAKERTACVRWLKPVARAEDPREFDKEEIVSVYELEGHPDYDYSYGDVVVRLSPAAVSTSDS-------ESAGELKQKTAPLENKSVKRRSGCKKEEDVSKGEACVDFSDLTWVGNI------------TGLRNGDIEVTWADGMVSTVGPQAIFVVGRDDXXXXSIAAGSEVSDDAAS--WETVNDDEMDALEIQEGVGPQNATDVNYEAEESEENNNSRRNPALS---IPLAALDFVTRLATGIFSRARKNVHPDFSESRGENEHQSRGIMHISEERDSGDESG-------------SQKSNVIDNGGLLSTHGDGEDLAV--------LDTEVPVSSNGAETLYNLSTDKSDIPTCFEGDTCSFKRFDITKDPLDHYFLGSDGQNNKARKWLKKVQQDWNILQNNLPDGIYVRVYEDRMDLLRAVIVGAYGTPYQDGLFCFDFHLPPEYPDVPPSAYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPVSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGSAEGEKNSLSYNENTFLLNCKTMMYLMRRP--PKDFEELVKEHFRRRGYYILKACDAYMKGSLIGSLTKEATVSNIDNSNLTSVGFKLMLAKIVPKLYLALN 1118          

BLAST of Ggra5929.t1 vs. uniprot
Match: A0A200Q025 (Ubiquitin-conjugating enzyme n=1 Tax=Macleaya cordata TaxID=56857 RepID=A0A200Q025_9MAGN)

HSP 1 Score: 277 bits (708), Expect = 6.290e-74
Identity = 309/1074 (28.77%), Postives = 482/1074 (44.88%), Query Frame = 0

Query:   98 NESLHKIEDLALRDRPLLRGNIVTNAANPS-QSAVVVDVNRRLTVRRV--SDISRTSSRRRATKNSFEVDQSQLGFFFGLCPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINAD-VRAGSLHKDEPLNEGF--HYPGQTVTASSETWTHSAVWIRGSYTGIDV-GTVTNVRMRQVEVHWIAQSNSAGETWEKDATSVELVDFSDITPLESFRQSCWIVGDKGFLVNPSSSTWKKGDLYHATVPFDNEAPRILNSDPDE----SMRNESQFDEDSEEWIEEDDD-------DRQSTFSDDSHAPQGRFFKAQRRARRKESHAEYIETSEHMTPPEVVRPEDV----------VQVIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGRKEDQSMHGNQRSRKDARRGVIVRVNHLEKTAIVKSERPDAHQSDVWDTPEEEV-SVYELTDD-DYDYQIGDTVLHLASKSPNTLMDWVGIILDHQRGEATVKWVSGAVTKAKCSELVRLDTEIGDEEGS-VSDSAYSGD----DDDSDAVRYFEAEAGFTSPELRLNWGSDTDSDNSDYG--MSDLDYGSYVVRSRLEGIFASNMFSTALTEEKMKKSIQLAVEYAVEAIEERKRINGSEGITLSRN---EVALVAGILVTRAVSELLARSLIGPHEESREKLEPGSKDVEEKLSKFSAALVTAFECLISGVVRQKQSQLENGTHENGNLDPSSSAMEVDNHES--------------HLSTDLPVMNNDRPLQVLENSSYNTVPKA----------ALPKFCVLEELLDFFYDPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARCRSLIHGDVARATLSTSKSLSGNQK-PYTSSGFKRSLEPLVHQLENAI 1106
            +E+   I D+ + DR  L G+IV  A++P+ QS +VVDVN  + +R    S +   SSR       F V             GD +V GH++G V ++  +V +   DGS+           ++ AD +R   + K+  L +G   +YPGQ V A S +   SA W+ G +    + GTVT V +  V ++WIA S S+G   E      E     ++  L  F  + W VGD   L +P+  +         ++   N++ R L++DP E    S   ES+   +  E   E  D       D  S   + + +P+     +     R+  H  +    + M    V R + V          + ++ T+T V + WQ+G TE  + +  L   D    ++F   Q V   E  S   +  S +  R GV+  VN  E+TA V+  +P A   D  +  +EEV SVYEL    DYDY  GD V+ L+  S ++     G  ++    +A        V K       +L+ +  +E+    SD ++ G+     D    V + +       P+     G D D ++   G  +SD D  S+      E +    M      EE+   S+Q A +   EA    + I  SE  +  RN    + L A   VTR  +   +R         R++++P S D                    SG   +   QL+        +D SSS++E +N  S               LS D  VM     L  L +   N++             +   F   ++ LD +Y      ++   R+L  + ++W  L+ +LPD I VR  E   +++RA I+G+ GTPY D +F FD  L   YP  PP V++ S G R+NPNLYE+GKVCLS+L TW G   E WDP +S++L+VL+SLQ  V   +PY+NEAG+  Q GT+ G+ NS+ YNEN FLL  K ++  L++   P D  E+   H++  G+ IL  C + + G +   +L+   S+S       TS GFK  L  ++ +L +A+
Sbjct:   86 SETTQSIGDIIVVDRGFLHGDIVAAASDPTGQSGIVVDVNIAVDLRAADGSIVKDVSSRDLKRVRDFTV-------------GDHIVLGHWLGRVEDVLDNVTMLFDDGSMC---------KVVKADPLRLKPVGKNI-LEDGHFPYYPGQRVRAISSSVFKSAKWLSGLWKASRMEGTVTKVTVGSVFIYWIA-SASSGYGSESATAPAEEQSPKNLKLLSCFAYANWQVGDWCHLSSPAPLS---------SIANKNDSSRRLSNDPVEDGLDSGDPESELSTEKYEGNSESMDLDTVPNLDVNSVNVESNASPKSSSCSSSLSVSREPIHEGWPIHRKKMRKVVVRRDKKVRRKEDNFERALVIVNTKTKVDVAWQNGKTEFGLVSTTLIPIDNPGDHEFFAEQYV--VEKASDDDDDVSSEVRRVGVVKSVNAKERTATVRWLKPVARPEDPREFDKEEVVSVYELEGHPDYDYCHGDVVVRLSPVSVSSQTGNSGKPVEESERQADSNGTKMDVKKHL--RHTKLEGKSKNEQDQDFSDLSWVGNITGLKDGDIEVTWADGMVSTVGPQAIYVVGRDDDEESIGGGSDVSDDDAASW------ETVNDDEMGDLENAEEEA--SLQNATDINPEA----ETIAHSEENSSGRNGPLAIPLAALGFVTRLATGFFSRG--------RKQIDPLSSDS-------------------SG---ENVPQLQGTIDSRAAVDGSSSSLESNNIGSCGAQTTHADAEEPGSLSEDTDVMKVAEVLCSLRSEEPNSLSMTLTGSSGDDPCSFRHFDTAKDPLDHYYLGESGQNNNGRRWLKKVQQDWTILQKNLPDDIYVRVYEDRMDLIRAVIVGAYGTPYQDGLFVFDFHLPPEYPQVPPSVYYHSGGWRINPNLYEEGKVCLSLLNTWTGRGNEVWDPSSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLSYNENTFLLNCKSMMYLLRKP--PKDFEELVKDHFRRRGHYILKACDAYMKGYLI-GSLTNDASISDKSNINSTSVGFKLMLAKIMPKLISAL 1077          

BLAST of Ggra5929.t1 vs. uniprot
Match: A0A1D6MMD1 (Putative ubiquitin-conjugating enzyme E2 23 n=8 Tax=Zea mays TaxID=4577 RepID=A0A1D6MMD1_MAIZE)

HSP 1 Score: 274 bits (701), Expect = 5.500e-73
Identity = 291/1082 (26.89%), Postives = 456/1082 (42.14%), Query Frame = 0

Query:   98 NESLHKIEDLALRDRPLLRGNIVTNAANPS-QSAVVVDVNRRLTVRRVSDISRTSSRRRATKNSFEVDQSQLGFFFGLCPGDTVVRGHYVGVVTNISADVYVNLSDGSLALLPSHDDRFPIINADVRAGSLHKDEPLNEGFHYPGQTVTASSETWTHSAVWIRGSYTGIDV-GTVTNVRMRQVEVHWIAQSNSAGETWEKDATSVELVDFSDITPLESFRQSCWIVGD-----------KGFLVNPSSSTWKKGDLYHATVPF----DNEAPRILNSDPD------ESMRNESQFDEDSEEWIEEDDDDRQSTFSDDSHAPQGR----------FFKAQRRARRKESHAEYIETSEHMTPPEVVRPEDVVQVIGTRTFVTLRWQDGTTEMNVPAMNLRTNDFEDGNDFRPGQIVGRKEDQSMHGNQRSRKDARRGVIVRVNHLEKTAIVKSERPDAHQSDVWDTP-EEEVSVYEL-TDDDYDYQIGDTVLHLASKSPNTLMDWVGIILDHQRGEATVKWVSGAVTKAKCSELVRLDTEIGDEEGSVSDSAYSGDDDDSDAVRYFEAEA--------------GFTSPELRLNWGSDTDSDNSDYGMSDLDYGSYVVRSRLEGIFASNMFSTALTEEKMKKSIQLAVEYAVEAIEERKRINGSEGITLSRNEVALVAGILVTRAVSELLARSLIGPHEESREKLEPGSKDVEEKLSKFSAALVTAFECLISGVVRQKQSQLENGTHENGNLDPSSSAMEVDNHESHLSTDLPVMNND------------RPLQVLENSSY-----------NTVPKAALPKFCVLEELLDFFYDPNPPGSSRNHRFLSVISKEWRRLRTSLPDGIIVRACERHDNILRAAIIGSEGTPYADIMFFFDVLLDERYPVHPPHVWFRSHGRRLNPNLYEDGKVCLSILGTWDGDDVESWDPRTSNLLRVLLSLQAFVFVEEPYYNEAGFGNQRGTSFGQNNSIGYNENVFLLCIKHILSSLQRSQNPADCYEMAAQHYKVAGNKILARCRSLIHGDVARATLSTSKSLSGNQKPYTSS-GFKRSLEPLVHQLENAI 1106
            +E    I+++ + DR  L G+ + +A++P+ Q  +V+DVN       V D+   +           V   +LG       GD VV G ++G V  +  +VYV+  DGS+  +   D +         AG +H D        YPGQ V ASS +   +  W+ G +    + GT+T V    V V+WIA ++ A +   + +  +E  +  D+T L  F  + W + D              +++    TW+       +VP     +++A   +++D        +S  +     +     I ++ +   S  S  +  PQ            FFK  +R ++++ +                  E  + +  T T V + WQDGT E  V A  L      + ++F P Q V  K    + G   S +  R G++  VN  ++TA V   +P  H  +  +    E VSVYEL    DYDY  GD V+ L    P                          +      E + LD  +   EG  +  +YS   D S   +  + E+              GF   E+ + WG   D   S  G  ++    YVV    +G                             ++++    +GS   T+  NE+ ++      +  S+ +  + IG         E GS   ++  S  +  L  AFE     V R        G     +LD SS AM  D  ESH S ++  + +D                V+EN S            N V       F VL+  LD  Y  N    +   +++  + +EW  L  +LPD I VR  E   ++LRA I+G+ GTPY D +FFFD  L   YP+ PP  ++ S G R+NPNLY DGKVCLS+L TW G   E WDP +S++L+VL+SLQ  V  E+PY+NEAG+  Q GT  G+ N++ YNEN +LL +K +L  L+R   P    +    H++  G+ IL  C + + G+V   TL+     +   K ++SS GFK  L  ++ +L  A+
Sbjct:  119 SEKTEDIDEVVVVDRSFLHGDFIASASDPTGQMGLVLDVNL------VVDLQGANG-----DMIKGVSSKELGRIREFNVGDYVVSGLWLGRVDGVLDNVYVSFDDGSVCKVNRADPKC----LKPAAGPIHPDTACP---FYPGQRVKASSSSVFKTCRWLNGFWRASRLEGTITKVESVAVLVYWIASAHFATD---QQSVPLEEQNPEDLTLLSRFSYTNWQLTDWCLSQRYTSCTNDTMIDSDKHTWQICTCSENSVPLSGIPESQADAQIDTDTGHRQTDVDSTADVLSMSDGDNSCIAKESETGTSASSISNEGPQDNATSRKKFRKVFFKKDKRTKKRDDNF-----------------ERALLIANTCTKVDVIWQDGTKECGVAATALIPIHSPNEHEFFPEQYVVDKVTNDV-GVDDSSEPRRVGLVRGVNAKDRTATVSWFKPPLHPEEPKEIACNEIVSVYELDVHPDYDYCYGDIVVRLPPVKP-------------------------VIESTNSKEQMELDKTLDSSEGFAA--SYSAPPDTSADEQLSQKESCLQFTSLSWAGNIVGFQDGEIEVIWG---DGSISKVGPHEI----YVVGREDDGA----------------------------SLDDGTASDGSSWETVDDNEMDVLHDS--AKGDSQNVPENSIGR--------ENGSFSSQDGSSVATGPLSVAFEF----VTRLASDLFARGRR---HLDGSSDAM--DEVESHWSNEISEIGDDIDKCNKNHVESPEHAAVIENDSSVEKSVDVVMADNPVHSECFKHFDVLQCPLDHHYLENTTQGTGGKKWVKKVQQEWSILEKNLPDYIYVRVFEDRMDLLRAVIVGASGTPYQDGLFFFDFHLPLEYPLVPPSAYYHSGGLRVNPNLYVDGKVCLSLLNTWTGRGNEVWDPSSSSILQVLVSLQGLVLNEKPYFNEAGYEKQVGTVEGEKNAVPYNENTYLLSVKSMLYILRRP--PLHFEDFVKSHFRKRGHYILKACHAYLQGNVV-GTLTDDACTTDRSKEHSSSVGFKLVLAKILPRLITAL 1077