Ggra10630.t1 (polypeptide) Gracilaria gracilis GNS1m male

BLAST of Ggra10630.t1 vs. uniprot
Match: A0A2V3J3X5 (Pullulanase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3J3X5_9FLOR)

HSP 1 Score: 1429 bits (3700), Expect = 0.000e+0
Identity = 684/990 (69.09%), Postives = 805/990 (81.31%), Query Frame = 0

Query:  203 WVDFIVDRACYGAANSICLLPRRGGDAWLEKDGTERVWNASFLE--EQCVQSEDRP--QYDDKYDHLPTFIITQSTSFVLRSLDDVKSMMNAYVDAENSIIVTTPVPVEWISPPKTCGRHSNPEVVVQLCKRSVQALLDEGKQSHDQVAGKVLRYRKSKQLSLTKLRLLFDDSVAAFDEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEKPTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEGENKRLFPGVEVIDPYSKCNTAHAGRGLIYGFDTTPVCERPKVETAEIIVYELHVRDMTIDKLSGIRNSGKFLGLAERGTRMKMKEPENRPDKHLTLWEQEAMPGVETAFSMLDKFSTGLDHIAQMGVTAVQIMPVQDFDNNEADDEAYRWGYMPVHFNSPDGWYASSTYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFNEEDIRRMHKLAAVIVFTSQGIPFIQAGQEMCRTKFDDENSYESPDEINMIRWSSKLKEWSIVQYYRGLILLRRAHPEIFCIETANEIHEKVVFYEDCGLRVPDRCIGYRLKGDPEKLLKRLQDTHHQAGWKGQDHEEESLRWSEVVVLLNATPLQVVFELPGKKGDVLWMQIVNAWTAGTRTINGPIIGSVCVGGRSAAILRRASPQECYNSQLEIRLAAVADAHCSFHGDDPLSRYAVGLEPQPTAEDQALKNNSISQRLQFQKRAETMREQGGGNVTEITD 1188
            W DF+VDRA YG+   I +LPRRGGDAWLE+D   R W+ S LE  +  +     P  Q  D +D LPTF+ITQ T  VLR LDD ++M++AYVD++NSI+V+ PVP++W+ PP+      N ++V+++ +R VQ LLD GK++ D + GK LR+ K+KQ++  K   +FD SVA F+EDFLVENVVVS+P F SV LHWETHE+ED+YLY+GSLGW Y+  +C+FRCFAP A  VSV+LY++PTGDAGR VVPMRRIPQGCWK T++++L+GR+YKLLAEG NKRLFPGVEVIDPYS+CNT H GRGL+YG D+T V  RP V+ +  IVYELH+RD+TIDK SGIR  GKFLGL ERGT +  K  ++   K LT W QE M GV   FS+LDKFSTGLDHI QMGVTAVQIMP+QDFDN+E+DD +YRWGYMPVHFNSPDGWYASST++ ARV EFK LVSA HKAGL+VIMDVVYNHTAEDSNE N++ARFSFNGLAPRYYYRTCGNTP+AH G+ TCG RE  +P CGECYSNGSGCGNEFRSESPMGRKFIIDSLKFW  EYGVDGFRFDLLGLID+ TI RA  ELK+I   I+VYGEPWIGGLSPIK T+KGSQRSKGF VFNDTFRNA+RGSPFDTEETF+MDGGRL EVKGGIIGSVD+F DSPLES+NYVECHDNRTLWDHL +Y RSRTD IKFNEED+RRMH+LAAV+V TSQG+PFIQAGQEMCRTKFDDENSYES DEINMIRW +KL EWS VQYYRGLILLRR+HPEIFC+ETA+EIH+KV FYEDCGL VP+RCI YR+KG+PE+L ++LQ+           H EESLRWSEVVVLLNATPL V FELPG+K D LWMQ V++  AGTR I GP +GSV V GRSAA+LRRASP E    QLE+RL++++DA+ SFHGDDPL+RY VGL+P+PT ED + +N+ ISQRL+F +R      Q   +   IT+
Sbjct:  524 WADFVVDRADYGSGKIISILPRRGGDAWLEQDEFVRQWSLSMLESLDTSIVKHHEPLKQRADDHDDLPTFLITQETKLVLRHLDDARTMLDAYVDSDNSIVVSAPVPLQWLMPPQLTQSQPNEDMVIKIAQRPVQGLLDVGKEADD-IEGKSLRFLKAKQVTPIKTGYVFDKSVAHFEEDFLVENVVVSVPGFVSVALHWETHENEDKYLYEGSLGWEYSSAKCIFRCFAPIASLVSVVLYDEPTGDAGRTVVPMRRIPQGCWKVTIIEDLKGRYYKLLAEGGNKRLFPGVEVIDPYSRCNTGHTGRGLVYGIDSTEVYPRPNVKPSAAIVYELHIRDITIDKCSGIRQRGKFLGLTERGTGLPNKVSKSE-SKVLTQWPQERMRGVNDEFSLLDKFSTGLDHIVQMGVTAVQIMPIQDFDNDESDDGSYRWGYMPVHFNSPDGWYASSTHTVARVVEFKHLVSALHKAGLKVIMDVVYNHTAEDSNELNLDARFSFNGLAPRYYYRTCGNTPIAHTGDRTCGVREHPKPHCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWAMEYGVDGFRFDLLGLIDLETIHRAATELKSIHPHILVYGEPWIGGLSPIKITDKGSQRSKGFGVFNDTFRNAVRGSPFDTEETFIMDGGRLPEVKGGIIGSVDLFTDSPLESLNYVECHDNRTLWDHLTYYTRSRTDNIKFNEEDLRRMHRLAAVLVLTSQGVPFIQAGQEMCRTKFDDENSYESSDEINMIRWMTKLNEWSTVQYYRGLILLRRSHPEIFCLETADEIHKKVAFYEDCGLCVPERCIAYRIKGNPERLFRQLQEEEPDVD--RDFHREESLRWSEVVVLLNATPLHVTFELPGRKTDTLWMQTVDSSAAGTRIIQGPHVGSVTVSGRSAAVLRRASPTEQEVGQLEMRLSSISDAYSSFHGDDPLTRYTVGLDPRPTVEDVSQRNDLISQRLEFDRRRSVNPSQATQDSDMITE 1509          

BLAST of Ggra10630.t1 vs. uniprot
Match: R7Q3N6 (Pullulanase, Pul1 n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7Q3N6_CHOCR)

HSP 1 Score: 1213 bits (3138), Expect = 0.000e+0
Identity = 600/1038 (57.80%), Postives = 746/1038 (71.87%), Query Frame = 0

Query:  193 APYSPHISLSWVDFIVDRACYGAANSICLLPRRGGDAWLEKDGTERVW-----NASFLEEQCVQSEDRPQ--------YDDKYDHLPTFIITQSTSFVLRSLDDVKSMMNAYVDAENSIIVTTPVPVEWISPPKTCGRHSNPEVVVQLCKRSVQALLDEGKQSHDQVAGKVLRYRKSKQLSLTKLRLLFDDSVAAFDEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEKPTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEGENKRLFPGVEVIDPYSKCNTAHAGRGLIYGFDTTPVCERPKVETAEIIVYELHVRDMTIDKLSGIRNSGKFLGLAERGTRMK-MKEPENRPD---KHLTLWEQEAMPGVETAFSMLDKFSTGLDHIAQMGVTAVQIMPVQDFDNNEADDEAYRWGYMPVHFNSPDGWYASSTYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFNEEDIRRMHKLAAVIVFTSQGIPFIQAGQEMCRTKFDDENSYESPDEINMIRWSSKLKEWSIVQYYRGLILLRRAHPEIFCIETANEIHEKVVFYEDCGLRVPDRCIGYRLKGDPEKLLKRLQDTHHQAGWKGQDHEEESLRWSEVVVLLNATPLQVVFELPGKKGDVLWMQIVNAWTAGTRTINGPIIGSVCVGGRSAAILRRASPQECYNSQLEIRLAAVADAHCSFHGDDPLSRYAVGLEPQPTAED-------QALKNNSISQRLQFQKRAETMR--EQGGGNVTEITDAKT----MCSKGKPSP 1200
            AP    I+ +WVDF +DRA YGA  SI L+PR+  + W E+D   RVW     N +          D P         + + +  +P F I Q +S +LR L++VKSMM AYVD+E SII+ +PVPV W+SPP      +  + VV++C  +            D   G VL  RK  +++ T+ RL F++    F+EDFL+ENV V++P FDSV L W  HED D+Y Y G+LGW Y   +C F+CFAP AD + VILY  P G  GR  VPMRRIP+GCWKA V ++L+G++YKL A GEN RLFPGVEVIDPYS+CNT+H GRGLI+GF+ T +   P +  +E I+YELHVRD+TID+ S +   GKFLG+ ERGT+MK +       D   K LT W+QE +P +E     LDKFS+ LDHI QMGV  +QI+P+QDFDN+E DD +Y WGYMPVHFNSPDGWYASST S ARV EFK+LV A HKAGL+VIMDVVYNHTAEDSNE+N++ARFSFNGLAPRYYYRTCGNTPVA+ G+STC  R+P EPRCG CYSNGSGCGNE RSESPMGRKFIIDSLK+WVTEY VDGFRFDLLGLIDV+T++ A  EL+ I+ ++++YGEPW GGLSPI+ T KG QRS+GF VFN+TFR+AIRGSPF TEETFVMDG RLTE+KGGIIGSVD F D P E++NYVECHDN TLWDH   YIRSRTD I F E D+RRMH+LAA IVFTSQGIPF+Q GQEMCRTKFD ENSYESPD+INM+RW +K  EW+ VQYYRGLILLRRAHPE+FC  TA+EIHE +VFYED GL VP+RCIGYR+ G+  KL +RL  T+  A ++     EES++W+EVVVLLN TP QVVFELP  + D +W+++V+A  +GTR +  P+IG++ V GRSA +LRRAS +E  + QL +RL +++D + SFHGDDP+++YAVGL  Q + ++       +AL+     QR++ Q  A  +   +    N  E  D K       SK +PSP
Sbjct:  540 APMPDSITRAWVDFEIDRARYGAGASISLVPRKNREMWSERDEPTRVWRNDMCNRTSRTLDVALVTDAPSTEIGGECPFQEDFS-VPMFTIVQGSSLILRKLEEVKSMMTAYVDSEESIIIASPVPVAWVSPPNRGRSQAISDTVVRVCSAATNGFRSADGHIEDDTTGIVLPIRKFHEVAPTQFRLTFNEKAVRFEEDFLIENVSVTVPGFDSVRLTWRIHEDWDKYHYTGTLGWEYEVTKCSFKCFAPTADNMYVILYNSPHGKEGRRAVPMRRIPEGCWKAIVEEDLKGKYYKLRAGGENPRLFPGVEVIDPYSRCNTSHTGRGLIFGFEKTAIQPGPCIPASETIIYELHVRDLTIDENSSVIKRGKFLGVTERGTKMKAVSSGSGTGDTSAKPLTPWKQELIPTLEVGMQSLDKFSSALDHIVQMGVNTIQILPIQDFDNDENDDSSYGWGYMPVHFNSPDGWYASSTSSVARVTEFKQLVDAVHKAGLKVIMDVVYNHTAEDSNEYNLDARFSFNGLAPRYYYRTCGNTPVAYTGDSTCAMRKPDEPRCGACYSNGSGCGNELRSESPMGRKFIIDSLKYWVTEYKVDGFRFDLLGLIDVDTLEMAGSELRQINPNVMIYGEPWAGGLSPIRITAKGCQRSRGFGVFNNTFRDAIRGSPFGTEETFVMDGSRLTEIKGGIIGSVDDFCDFPTETVNYVECHDNYTLWDHFRFYIRSRTDDIVFTENDMRRMHRLAAAIVFTSQGIPFMQLGQEMCRTKFDVENSYESPDKINMVRWEAKQSEWTTVQYYRGLILLRRAHPELFCKSTADEIHEGLVFYEDLGLAVPERCIGYRILGNSAKLYQRLLTTNPDADYEAL--REESMKWTEVVVLLNPTPSQVVFELPDHERDAIWVEVVDATHSGTRGLRMPMIGAIEVQGRSACVLRRASEREEVDLQLRLRLNSISDCYSSFHGDDPITKYAVGLSSQRSEKELEDFKRLKALRERFEEQRVRLQDNATFVPSPKMTEANAIETLDHKHGEHHKQSKSQPSP 1574          

BLAST of Ggra10630.t1 vs. uniprot
Match: A0A7S3E8R2 (Pullulanase n=4 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3E8R2_9RHOD)

HSP 1 Score: 877 bits (2267), Expect = 7.860e-296
Identity = 448/917 (48.85%), Postives = 578/917 (63.03%), Query Frame = 0

Query:  202 SWVDFIVDRACYGAANSICLLPRRGGDAWLEKDGTERVWNASFLEEQCVQSEDRPQYDDKYDHLPTFIITQSTSFVLRSLDDVKSMMNAYVDAENSIIVTTPVPVEWISPPKTCGRHSNPEVVVQLCKRSVQALLDEGKQSHDQVAGKVLRYRKSKQLSLTKLRLLFDDSVAAFDEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEK---PTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEGENKRLFPGVEVIDPYSKCNTAH--AGRGLIYGFDTTPVCERPKVETAEIIVYELHVRDMTIDKLSGIRNSGKFLGLAERGTRMKMKEPENRPDKHLTLWEQEAMPGVETAFSMLDKFSTGLDHIAQMGVTAVQIMPVQDFDNNEADDEAYRWGYMPVHFNSPDGWYASSTYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFNEEDIRRMHKLAAVIVFTSQGIPFIQAGQEMCRTKFDDENSYESPDEINMIRWSSKLKEWSIVQYYRGLILLRRAHPEIFCIETANEIHEKVVFYEDCGLRVPDRCIGYRLKGDPEKLLKRLQDTHHQAGWKGQDHEEESLRWSEVVVLLNATPLQVVFELPGKKGDVLWMQIVNAWTAGTRTINGPIIGSVCVGGRSAAILRRASPQE 1113
            ++  F VDR+ YG+   I L+ R+GG+ W  KD  +R+W A            RP + D Y      I  Q    ++R L+D    ++AYV+ + +I + + + ++W  P            +    K+S   +            GK ++  K ++ S     +  D      DEDF VE+  +S   F    L W  +++ D+Y Y+G LG  +   +C FR FAP AD VSV++Y+    P G  GR V PMRRIPQGCWKA +  NL+G++YKLLAEG +KRL+PGVEVIDPYS+CNTAH   GRGLIY  D T + +RPK  T+  ++YELH+RD TID  SG+   GKF+GL ERGT               T+W+++    V        K ST L+HI +MGV  VQIMP+QDFDN+E   E YRWGYMPVHFNSPDGWYASST + +RV EFKKLV   H  G++V+MDVVYNHT+ED NEFN+EARFSFNGLAPRYYYR C + PVA +G +TC      EP CG CYSNGSGCGNEFRS++PMGRKFIIDSLKFWV EY +DGFRFDL+GLID  T+ +A  ELK ID  I++YGEPW GG SPI AT KG QR +GF VFND+ R+A+RG  +  +ETF+MDGG   +VK GI+GS++ FA  P E+INY ECHDNRTLWD  +HY   RTD+I F + D  RM KLAA+I+FTSQG+PFIQ GQEMCRTK   ENSYESPD +NM++W  K +    V+YY+GLI +R AH  +F +  A++I + ++F+E+ GL+ P+RCI +R+  D            H  G           RWS+VVVLLN TPL+  F LP +  D LW  + +   AGT TI  P+   + V GRS  +LRR S  E
Sbjct:  506 AYPTFRVDRSLYGSGREILLIGRQGGEHWTAKDEPQRMWKAEGR---------RPNFVDPY------IFLQGHPHLVRRLEDAIGNLSAYVETDGTISLHSSMDLKWFHPN-----------IDDSSKKSSLIIKSAN--------GKAMKIVKVERKSSVLRVIHVDIQSQLLDEDFRVEDFTISARNFSPEVLTWRRYDNFDEYFYEGDLGLEFGIAECTFRTFAPGADHVSVVIYDTAVGPNGGGGRKVHPMRRIPQGCWKANIKANLKGKYYKLLAEGSDKRLYPGVEVIDPYSRCNTAHHTEGRGLIYPMDNTRIADRPKFPTSASVIYELHLRDSTIDPASGVNKKGKFMGLTERGT-------------STTVWDEKTGQNV--------KISTALEHILEMGVNTVQIMPIQDFDNDENSQEEYRWGYMPVHFNSPDGWYASSTSNFSRVVEFKKLVDCFHSNGIKVVMDVVYNHTSEDPNEFNLEARFSFNGLAPRYYYRNCVSHPVAASGHNTCAWHGMDEPHCGTCYSNGSGCGNEFRSDAPMGRKFIIDSLKFWVEEYKIDGFRFDLMGLIDQETMTQATAELKKIDDGILIYGEPWTGGPSPIVATVKGFQRDRGFGVFNDSLRDALRGGNWGIQETFLMDGGEAEKVKRGIMGSIEDFASQPTETINYAECHDNRTLWDQFSHYRELRTDSIVFTDSDFIRMGKLAALIIFTSQGLPFIQIGQEMCRTKGGVENSYESPDSVNMVQWKWKQERRRCVEYYKGLIKMRLAHQHLFAMTYASDIRKNLIFFEELGLQPPERCIAFRISSD------------HPEG-------PSKARWSQVVVLLNPTPLEQRFWLPNEP-DFLWGPVADDVAAGTDTIGAPVQDQISVRGRSGCVLRRISRVE 1347          

BLAST of Ggra10630.t1 vs. uniprot
Match: A0A5J4YVH4 (Pullulanase n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YVH4_PORPP)

HSP 1 Score: 877 bits (2267), Expect = 1.020e-290
Identity = 466/985 (47.31%), Postives = 611/985 (62.03%), Query Frame = 0

Query:  181 PSNSQPLFSTFHAPYSPHIS---LSWVDFIVDRACYGAANSICLLPRRGGDAWLEKDGTERVWNASFLEEQCVQSEDRPQYDDKYDHLPTFIITQSTSFVLRSLDDVKSMMNAYVDAENSIIVTTPVPVEWISPPKTCGRHSNPEVVVQLCKRSVQALLDEGKQSHDQVAGKVLRYRKSKQLSLTKLRLLFDDSVAAFDEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEKPTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEG--ENKRLFPGVEVIDPYSKCNTAHAGRGLIYGFDTTPVCERPKVETAEIIVYELHVRDMTIDKLSGIRNSGKFLGLAERGTRMKMKEPE-----NRPDKHLTLWEQEAMPGVETAFSM----------------------------------LDKFSTGLDHIAQMGVTAVQIMPVQDFDNNEA--DDEAYRWGYMPVHFNSPDGWYASSTYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFNEEDIRRMHKLAAVIVFTSQGIPFIQAGQEMCRTKFDDENSYESPDEINMIRWSSKLKEWSIVQYYRGLILLRRAHPEIFCIETANEIHEKVVFYEDCGLRVPDRCIGYRLKGDPEKLLKRLQDTHHQAGWKGQDHEEESLR-----WSEVVVLLNATPLQVVFELPGKKGDVLWMQIVN-AWTAGTRTINGPIIGSVCVGGRSAAILRRASPQE 1113
            PS+S   F  F  P+   ++   LS+V F + RAC G  N I LLPR GG AW ++      W+ +      +  E  P+                   ++  L D    M A V  +N + ++T +P  W +P +     +N + V +      Q +LD           KVL    S  +    +R  F+     FDEDF VE   VS+       L W  +E+ED Y Y G+LGW Y+  + VFRCFAP+A ++SV+LY  P+G  GR +V MRRI  G WKA +  +L+GR+YKLLA    ++ R FPGVEVIDPYS+CNT+H GRGLI+G DTTPV +RP V     I+YE+HVRDMTID+ SGI   GKFLGL ERG+ ++  E E     N   K  T+ EQ    GV  + +                                   +   ST LDHI +MGVTAVQI+PVQDFDN+E+  D   Y WGYMPVHFNSPDGWYA+ T S+ARV EFK+LVSA H  GL+VIMDVVYNHTAED+NEFN+EARFSFNGLAPRYYYR CGNTP++    +TC  R  HE RCG C+SNGSGCGNEFRSE+PMGRKF++DSL++WV+EY VDGFRFDLL L+D  T+     E++ ID +I++YGEPW GG++PI  TEKG+QR +GF+VFNDTFR+A+RGS +  +ETF+MDGGR+ EVK GI+GS+D+FADSPLESINY+ECHDNRTL+D   HY+ +R D I + + D  RM +L A+IV TSQG+PFI AGQEM RTK   ENSY SPD+INM+RW+ K +    V+Y++ LI LRR HP++F +  A E+ E++ F+ED  L VP+RCI Y ++G         ++  +  G++      E  R     WS+VVVLLN TP+   F LPG      W+ +V+  + +  R +  P++  V V GRS  +LR A+P +
Sbjct:  839 PSSSSDGFG-FGQPHMDVVNIDGLSFVVFSIPRACLGCGNRIKLLPRHGGAAWTDRGAHTVTWDCTRHPRDRMLLEGFPE-------------------LVAELFDAVGDMTASVIDDNIVEISTSLPASWFAPEQD---PANVKAVFKGASSGHQNVLD---------LNKVLLSMTS--VDAWTMRATFEPRAVRFDEDFPVELYSVSVLNMHDARLRWPVYENEDAYYYDGTLGWEYSPSETVFRCFAPSAAELSVVLYSAPSGQQGRELVSMRRIMGGVWKARIQGDLKGRYYKLLALSTPDDTRRFPGVEVIDPYSRCNTSHTGRGLIFGRDTTPVTDRPNVPPQNAIIYEIHVRDMTIDQQSGILMRGKFLGLTERGSMLQEGEQEPSAGQNCETKVGTVTEQARKLGVADSVAARASPSKRAQKAENGVSQGEQLDHLPLPDIIDDVEITPLSTALDHIIEMGVTAVQILPVQDFDNDESSTDGNQYWWGYMPVHFNSPDGWYATDTTSAARVSEFKQLVSALHDVGLKVIMDVVYNHTAEDANEFNLEARFSFNGLAPRYYYRNCGNTPLSVGDHNTCANRGKHEKRCGTCFSNGSGCGNEFRSEAPMGRKFLLDSLRYWVSEYKVDGFRFDLLALVDTETVAMLAREMQKIDPNIMLYGEPWTGGMTPIAPTEKGTQRGRGFSVFNDTFRDALRGSTWSVDETFLMDGGRIDEVKRGILGSIDVFADSPLESINYIECHDNRTLYDQFQHYVANRQDDITYTDADYERMARLGALIVLTSQGVPFIHAGQEMMRTKQGVENSYCSPDDINMVRWAWKRERLMCVRYHQHLIRLRRTHPQLFSMTNAAEVRERITFFEDLDLIVPERCIAYHMQG--------YKEGEYAHGYRIDSLSSEERRRIEQQWSQVVVLLNPTPMGTDFGLPGAAHQQAWVCMVDDEFASCERAVRAPVMIQVHVPGRSGMLLRAAAPDD 1781          

BLAST of Ggra10630.t1 vs. uniprot
Match: A0A7S3E758 (Hypothetical protein n=1 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3E758_9RHOD)

HSP 1 Score: 751 bits (1940), Expect = 4.190e-253
Identity = 379/743 (51.01%), Postives = 481/743 (64.74%), Query Frame = 0

Query:  202 SWVDFIVDRACYGAANSICLLPRRGGDAWLEKDGTERVWNASFLEEQCVQSEDRPQYDDKYDHLPTFIITQSTSFVLRSLDDVKSMMNAYVDAENSIIVTTPVPVEWISPPKTCGRHSNPEVVVQLCKRSVQALLDEGKQSHDQVAGKVLRYRKSKQLSLTKLRLLFDDSVAAFDEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEK---PTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEGENKRLFPGVEVIDPYSKCNTAH--AGRGLIYGFDTTPVCERPKVETAEIIVYELHVRDMTIDKLSGIRNSGKFLGLAERGTRMKMKEPENRPDKHLTLWEQEAMPGVETAFSMLDKFSTGLDHIAQMGVTAVQIMPVQDFDNNEADDEAYRWGYMPVHFNSPDGWYASSTYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFNEEDIRRMHKLAAVIVFTSQGIPFIQAGQEMCRTK 939
            ++  F VDR+ YG+   I L+ R+GG+ W  KD  +R+W A            RP + D Y      I  Q    ++R L+D    ++AYV+ + +I + + + ++W  P            +    K+S   +            GK ++  K ++ S     +  D      DEDF VE+  +S   F    L W  +++ D+Y Y+G LG  +   +C FR FAP AD VSV++Y+    P G  GR V PMRRIPQGCWKA +  NL+G++YKLLAEG +KRL+PGVEVIDPYS+CNTAH   GRGLIY  D T + +RPK  T+  ++YELH+RD TID  SG+   GKF+GL ERGT               T+W+++    V        K ST L+HI +MGV  VQIMP+QDFDN+E   E YRWGYMPVHFNSPDGWYASST + +RV EFKKLV   H  G++V+MDVVYNHT+ED NEFN+EARFSFNGLAPRYYYR C + PVA +G +TC      EP CG CYSNGSGCGNEFRS++PMGRKFIIDSLKFWV EY +DGFRFDL+GLID  T+ +A  ELK ID  I++YGEPW GG SPI AT KG QR +GF VFND+ R+A+RG  +  +ETF+MDGG   +VK GI+GS++ FA  P E+INY ECHDNRTLWD  +HY   RTD+I F + D  RM KLAA+I+FTSQG+PFIQ GQEMCRTK
Sbjct:  219 AYPTFRVDRSLYGSGREILLIGRQGGEHWTAKDEPQRMWKAEGR---------RPNFVDPY------IFLQGHPHLVRRLEDAIGNLSAYVETDGTISLHSSMDLKWFHPN-----------IDDSSKKSSLIIKSAN--------GKAMKIVKVERKSSVLRVIHVDIQSQLLDEDFRVEDFTISARNFSPEVLTWRRYDNFDEYFYEGDLGLEFGIAECTFRTFAPGADHVSVVIYDTAVGPNGGGGRKVHPMRRIPQGCWKANIKANLKGKYYKLLAEGSDKRLYPGVEVIDPYSRCNTAHHTEGRGLIYPMDNTRIADRPKFPTSASVIYELHLRDSTIDPASGVNKKGKFMGLTERGT-------------STTVWDEKTGQNV--------KISTALEHILEMGVNTVQIMPIQDFDNDENSQEEYRWGYMPVHFNSPDGWYASSTSNFSRVVEFKKLVDCFHSNGIKVVMDVVYNHTSEDPNEFNLEARFSFNGLAPRYYYRNCVSHPVAASGHNTCAWHGMDEPHCGTCYSNGSGCGNEFRSDAPMGRKFIIDSLKFWVEEYKIDGFRFDLMGLIDQETMTQATAELKKIDDGILIYGEPWTGGPSPIVATVKGFQRDRGFGVFNDSLRDALRGGNWGIQETFLMDGGEAEKVKRGIMGSIEDFASQPTETINYAECHDNRTLWDQFSHYRELRTDSIVFTDSDFIRMGKLAALIIFTSQGLPFIQIGQEMCRTK 906          

BLAST of Ggra10630.t1 vs. uniprot
Match: A0A1X6PAN7 (Aamy domain-containing protein n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6PAN7_PORUM)

HSP 1 Score: 746 bits (1926), Expect = 5.840e-248
Identity = 439/1054 (41.65%), Postives = 581/1054 (55.12%), Query Frame = 0

Query:  206 FIVDRACYGAANSICLLPRRGGDAWLEKDGTERVWN-----------ASFLEEQCVQSEDRPQYDDKYDHLPTFIITQSTSFVLRSLDDVKSMMNAYVDAENSIIVTTPVPVEWI---SPPKTCGRHSNPEVVVQLCKRSVQALLDEGKQSHDQVAGKVLRYRKSKQLSLTKLRLLFDDSVA---AFDEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEKPTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEGENKRLFPGVEVIDPYSKCNTAHAGRGLIYGFD----TTPVCERPKVETAE-IIVYELHVRDMTIDKLSGI-RNSGKFLGLAERGTRMK-----MKEPENRPDKHLTLWEQEAMPGVETAFSMLDK--FSTGLDHIAQMGVTAVQIMPVQDFDNNEADDEA---YRWGYMPVHFNSPDGWYAS-STYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIR-----SRTDTIKFNEEDI----------------------------------------------------RRMHKLAAVIVFTSQGIPFIQAGQEMCRTKFDDENSYESPDEINMIRWSSKLKEWSIVQYYRGLILLRRAHPEIFCIETANEIHEKVVFYEDCGLRVPDRCIGYRLKGDPEKLLKRLQDTHHQAGWKGQDHEEESLRWSEVVVLLNATPLQVVFELPGKKGDVLWMQIVNAWTAGTRTINGPIIGSVCVGGRSAAILRRASPQECYNSQLEIRLAAVADAHCSFHGDDPLSRYAVGLEPQPTAEDQALKNNSISQRLQF 1168
            F+VDR  +G  + + L+PR    A ++  G    W+           A+ ++       D P         P  ++ Q    +L SL    S +   VD+   + VTT  P+EW+   +P K     ++P    +L  R  QA L                +   ++ S T++ L   ++VA      ED+LVE VV       ++ L W  H + D Y Y G++G   T  + VFR FAP AD V+V+LY+ P  DA R  VPMRRIPQGCWKA V  +L G +YKLLA G +  LFPGVEVIDPYS+CNT H GRGLI   D       V  RP V   + ++++ELH+RD T+D  SG+  + GK+LGL +R T +        +P+   D        +       A    D    ST L H+ +MG TAVQ++P+QDFDNNE  DEA   Y WGYMPVHF SPDGWYAS S   S RV+E K+LVSA H+AGLRVI+DVV+NHTAED NE N++ARFSFNG+APRYYYRTCGNTP++ +G  TC       P CG CYSNGSGCGNE RSE+PM RKFIID L++W TEYG+DGFRFDL+GL+DV T+  A   LKAID  I++YGEPW GG++P+  T KG+QR +GF VFNDTFR+A+RGSPF     FV+DG  + EVK GI+GSVD FAD+P E INYV CHDNRTL+D L    R     +  D     EE +                                                    RR   LA  IV T+QG+PF+Q GQE  RTK  D NSY SPD +N +RW+ K    + V++  GL+ LRR HPEIF + TA  + E+++F+E  G+ VP  C+ YR+ G    ++ R       AG       E++ RW+ V+VL+N       F LP  + + LW+ +V    AG   +  P+   + V   S  +LRR S  E   + ++ RLA ++D        D  SRYAVGLE +  A + A+   ++  R  F
Sbjct:  109 FLVDRGRFGDGSRLRLVPRHAERA-VDDAGATVTWDVDAADAAFWIPATVVDALVAGGGDTPP--------PALLLVQGHGSLLTSLASEPSPVVVSVDSPWDVAVTTFCPLEWLDGTAPAKPWDLAASPSTRPKLTLRHGQAELP---------------WVTWERPSPTEVVLRLSEAVADGRGLHEDYLVEEVVARATGIPTLALQWRRHANVDAYYYPGAMGCMCTASETVFRVFAPTADGVAVVLYDGPGDDASRREVPMRRIPQGCWKAIVPSSLGGTYYKLLASGADTLLFPGVEVIDPYSRCNTHHTGRGLILAPDDPAVVATVAPRPPVGPDDAVVIWELHLRDATVDPASGVVGHRGKYLGLTQRDTVLVPGAPFAADPKGMVDVSAGADAADTAKPAPAANGEADAPPVSTALAHLVEMGATAVQLLPLQDFDNNE--DEAVGEYAWGYMPVHFFSPDGWYASGSRTDSTRVRELKELVSAMHEAGLRVILDVVFNHTAEDVNERNLDARFSFNGVAPRYYYRTCGNTPLSASGHRTCALTLAGAPTCGGCYSNGSGCGNEMRSEAPMARKFIIDCLRYWATEYGMDGFRFDLMGLVDVPTLTAASTALKAIDDGILLYGEPWTGGMTPMAPTYKGTQRGRGFGVFNDTFRDALRGSPFHVSSCFVLDGVCVDEVKRGIMGSVDDFADAPTEVINYVACHDNRTLYDQLWEIYRGGGEDTGADVAPPEEEPVGASAVKDIGPAPDATHLSPDAATAVAXXXXXXXXXXXXXXXXXXXXXVPALLRRCAALATAIVLTAQGVPFLQLGQEFFRTKGGDHNSYISPDAVNAVRWARKAAHAAAVRFVAGLVALRRGHPEIFALPTAGLVRERLLFFETMGVPVPAGCVAYRVDGCAADVVARAA----AAGASAATANEQAARWTAVLVLINPCRTAQAFALP--EAEQLWVPVVAGGHAGLSPVGAPVRRQLGVAPTSLTVLRRCSAAEADAAAVDDRLAVISDPGVVPADGDCTSRYAVGLERERGAAEAAVMEENVRLRRAF 1130          

BLAST of Ggra10630.t1 vs. uniprot
Match: A0A7V5M1D9 (Aamy domain-containing protein n=1 Tax=bacterium TaxID=1869227 RepID=A0A7V5M1D9_9BACT)

HSP 1 Score: 732 bits (1889), Expect = 3.780e-247
Identity = 395/794 (49.75%), Postives = 499/794 (62.85%), Query Frame = 0

Query:  376 DEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEKPTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEGENKRLFPGVEVIDPYSKCNTAHAGRGLIYGFDTTPVCERPKVETAEIIVYELHVRDMTIDKLSGIRNS--GKFLGLAERGTRMKMKEPENR-----------------PDKHLTLWEQEAMPG--------------VETAFSMLDKFSTGLDHIAQMGVTAVQIMPVQDFDNNEADDEAYRWGYMPVHFNSPDGWYASSTYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIRSRTDT-IKFNEEDIRRMHKLAAVIVFTSQGIPFIQAGQEMCRTKFDDENSYESPDEINMIRWSSKLKEWSIVQYYRGLILLRRAHPEIFCIETANEIHEKVVFYEDCGLRVPDRCIGYRLKGDPEKLLKRLQDTHHQAGWKGQDHEEESLRWSEVVVLLNATPLQVVFELPGKKGDVLWMQIVNAWTA---GTRTINGPIIGSVCVGGRSAAILRRASPQECYNSQLEIRLAAVADAHCS 1132
            DEDF VE V+V +  F      W  +E+ D+Y Y G+LGW YT+    FR FAP AD V + LY +P G  GR   PMRRIPQG WK  V  +LQG +Y  LAEG NKRLFPGVEVIDPYS+CNTAH GRGLI+G D T V +RP +     I+YE+H+RD TID+ SG+     GK+LGLA++GT++      +R                 PD     +E++  P               V       D+ ST LDH+  +GV A+QIMPVQDFDN+E++   YRWGYMPVHF SPDGWYAS T  ++RV+E K+L+   H+AGL+VI+D+V NHTAED NEFN+EARFSFNGLAPRYYYR     P    G S              CY+NGSGCGNEFRSESPMGRKFI+D L +W TEY VDGFRFDL+GL+D  T++ A  +L A+D +I+VYGEPW  G +PI  T KG+QR  GF VFNDTFRNA+RGS    EE F++DGG +  VK GI+GS+D FA SPLE INYVECHDNRTLWDHL + IR R D  I +   D+ R+ KLAAVI+ TSQGIPF+ +GQEM R+KF  ENSY+S D+IN +RW  K+  + +V Y RGL+ LRRA P+IF +  A ++   + FYE  GL VP+RC+ YR+  D              AG + + +  E   W  V +L N TP+  VF LP +  D L   IVN   A      ++    IG   V GRSA +LR  +  E   + +E RL A+++ + S
Sbjct:   19 DEDFPVEEVMVHLKGFSPSRGAWRRYENVDEYYYSGALGWHYTKSATTFRVFAPTADLVVLCLYREPVGTVGRQAFPMRRIPQGVWKIVVHGDLQGLYYTYLAEGVNKRLFPGVEVIDPYSRCNTAHNGRGLIFGEDHTEVADRPCIPPEHTIIYEVHLRDFTIDECSGVPEKLRGKYLGLAQQGTQLPEHWVRSRIVSSAGRAALFLRDRATPDA----FEEDVCPSTVEEDVYTAPVLTEVAAQVDWTDR-STCLDHVRFLGVNALQIMPVQDFDNDESNPLEYRWGYMPVHFFSPDGWYASCTSDASRVRELKQLIDTLHRAGLKVILDMVLNHTAEDPNEFNLEARFSFNGLAPRYYYRFW---PDGSWGVSEA------------CYANGSGCGNEFRSESPMGRKFILDMLWYWATEYKVDGFRFDLMGLMDRETLRIAAAKLHALDPNILVYGEPWSAGETPIIITGKGAQRHLGFGVFNDTFRNALRGSNQGIEENFLLDGGCIEAVKQGILGSIDDFAASPLEVINYVECHDNRTLWDHLWYVIRERADANITYTAADVLRVCKLAAVILLTSQGIPFLHSGQEMARSKFGVENSYQSGDDINRLRWERKIDFYGLVVYVRGLVQLRRARPDIFSMSDAQQVRRCIAFYEALGLPVPERCLAYRIIAD--------------AG-QSETNAIEDDPWRCVALLFNPTPVAQVFPLPPEAKDELMTVIVNDLEAPRDAGASLGEYHIGWASVPGRSAMVLRLCTESERERALIETRLNAISEPYSS 777          

BLAST of Ggra10630.t1 vs. uniprot
Match: M1VKA6 (Pullulanase n=1 Tax=Cyanidioschyzon merolae (strain 10D) TaxID=280699 RepID=M1VKA6_CYAM1)

HSP 1 Score: 731 bits (1887), Expect = 7.640e-235
Identity = 395/794 (49.75%), Postives = 498/794 (62.72%), Query Frame = 0

Query:  376 DEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEKPTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEGENKRLFPGVEVIDPYSKCNTAHAGRGLIYGFDTTPVCERPKVETAEIIVYELHVRDMTIDKLSGIRNS--GKFLGLAERGTRMKMKEPENR-----------------PDKHLTLWEQEAMPG--------------VETAFSMLDKFSTGLDHIAQMGVTAVQIMPVQDFDNNEADDEAYRWGYMPVHFNSPDGWYASSTYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIRSRTDT-IKFNEEDIRRMHKLAAVIVFTSQGIPFIQAGQEMCRTKFDDENSYESPDEINMIRWSSKLKEWSIVQYYRGLILLRRAHPEIFCIETANEIHEKVVFYEDCGLRVPDRCIGYRLKGDPEKLLKRLQDTHHQAGWKGQDHEEESLRWSEVVVLLNATPLQVVFELPGKKGDVLWMQIVNAWTA---GTRTINGPIIGSVCVGGRSAAILRRASPQECYNSQLEIRLAAVADAHCS 1132
            DEDF VE V+V +  F      W  +E+ D Y Y G+LGW YT+    FR FAP AD V + LY +P G  GR   PMRRIPQG WK  V  +LQG +Y  LAEG NKRLFPGVEVIDPYS+CNTAH GRGLI+G D T V +RP +     I+YE+H+RD TID+ SG+     GK+LGLA++GT++      +R                 PD     +E++  P               V       D+ ST LDH+  +GV A+QIMPVQDFDN+E++   YRWGYMPVHF SPDGWYAS T  ++RV+E K+L+   H+AGL+VI+D+V NHTAED NEFN+EARFSFNGLAPRYYYR     P    G S              CY+NGSGCGNEFRSESPMGRKFI+D L +W TEY VDGFRFDL+GL+D  T++ A  +L A+D +I+VYGEPW  G +PI  T KG+QR  GF VFNDTFRNA+RGS    EE F++DGG +  VK GI+GS+D FA SPLE INYVECHDNRTLWDHL + IR R D  I +   D+ R+ KLAAVI+ TSQGIPF+ +GQEM R+KF  ENSY+S D+IN +RW  K+  + +V Y RGL+ LRRA P+IF +  A ++   + FYE  GL VP+RC+ YR+  D              AG + + +  E   W  V +L N TP+  VF LP +  D L   IVN   A      ++    IG   V GRSA +LR  +  E   + +E RL A+++ + S
Sbjct: 1003 DEDFPVEEVMVHLKGFSPSRGAWRRYENVDDYYYSGALGWHYTKSATTFRVFAPTADLVVLCLYREPVGTVGRQAFPMRRIPQGVWKIVVHGDLQGLYYTYLAEGVNKRLFPGVEVIDPYSRCNTAHNGRGLIFGEDHTEVADRPCIPPEHTIIYEVHLRDFTIDECSGVPEKLRGKYLGLAQQGTQLPEHWVRSRIVSSAGRAALFLRDRATPDA----FEEDVCPSTVEEDVYTAPVLTEVAAQVDWTDR-STCLDHVRFLGVNALQIMPVQDFDNDESNPLEYRWGYMPVHFFSPDGWYASCTSDASRVRELKQLIDTLHRAGLKVILDMVLNHTAEDPNEFNLEARFSFNGLAPRYYYRFW---PDGSWGVSEA------------CYANGSGCGNEFRSESPMGRKFILDMLWYWATEYKVDGFRFDLMGLMDRETLRIAAAKLHALDPNILVYGEPWSAGETPIIITGKGAQRHLGFGVFNDTFRNALRGSNQGIEENFLLDGGCIEAVKQGILGSIDDFAASPLEVINYVECHDNRTLWDHLWYVIRERADANITYTAADVLRVCKLAAVILLTSQGIPFLHSGQEMARSKFGVENSYQSGDDINRLRWERKIDFYGLVVYVRGLVQLRRARPDIFSMSDAQQVRRCIAFYEALGLPVPERCLAYRIIAD--------------AG-QSETNAIEDDPWRCVALLFNPTPVAQVFPLPPEAKDELMTVIVNDLEAPRDAGASLGEYHIGWASVPGRSAMVLRLCTESERERALIETRLNAISEPYSS 1761          

BLAST of Ggra10630.t1 vs. uniprot
Match: A0A1Y1RKU5 (Pullulanase n=1 Tax=Candidatus Cloacimonetes bacterium 4572_55 TaxID=1971726 RepID=A0A1Y1RKU5_9BACT)

HSP 1 Score: 624 bits (1610), Expect = 6.850e-205
Identity = 360/908 (39.65%), Postives = 492/908 (54.19%), Query Frame = 0

Query:  204 VDFIVDRACYGAANSICLLPRRGGDAWLEKDGTERVWNASFLEEQCVQSEDRPQYDDKYDHLPTFIITQSTSFVLRSLDDVKSMMNAYVDAENSIIVTTPVPVEWISPPKTCGRHSNPEVVVQLCKRSVQALLDEGKQSHDQVAGKVLRYRKSKQLSLTKLRLL-FDDSVAAFDEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEKPTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEGENKRLFPGVEVIDPYSKCNTAHAGRGLIYGFDTTPVCERPKVETAEIIVYELHVRDMTIDKLSGIRNSGKFLGLAERGTRMKMKEPENRPDKHLTLWEQEAMPGVETAFSMLDKFSTGLDHIAQMGVTAVQIMPVQDFDNNEADDEAYRWGYMPVHFNSPDGWYASSTYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDT---EETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFNEEDIRRMHKLAAVIVFTSQGIPFIQAGQEMCRTKFDDENSYESPDEINMIRWSSKLKEWSIVQYYRGLILLRRAHPEIFCIETANEIHEKVVFYE-DCGLRVPDRCIGYRLKGDPEKLLKRLQDTHHQAGWKGQDHEEESLRWSEVVVLLNATPLQVVFELPGKKGDVLWMQIVNAWTAGTRTINGPIIGSVCVGGRSAAIL 1106
            + F++D+  YG+   I LLPR+G   WLEKD  +R+W  +   E  +   D   + +  D  P                    +  A++DA   + V  P+ + +           +PE         V+ + D+G+    Q            ++    L L+   DS  AFD    +    V +  F    L +    D  +Y+    LG  Y   Q +FR FAP A +V++ +Y++P  D G+   P+     G W+  + +NL+ ++Y    +G +    P  E+IDPYS+CNTAH GRG+I   D T + +RP     + I+YELHVRD +ID  SGI   G +LG+ E+G   K +                             +  TGLDH+ +MGV  VQIMP+QDFDNNE+ +E Y WGYMPVHFNSPDGWYA+   ++ RV+EFKKLV A H  G+RVIMDVVYNHTAE     N   ++SFNG+AP YYYR                       R    + NGSGCGNEFRSE+PM RKFI+DS+ +WV EY VDGFRFDL+GLID+ T+ +  E L  ID +I+VYGEPW  G +PI  T KG QR  GF VFND FR+A++GSPFD     ETF++ G R++E+K GI GS+  FA  PLES+NY+ECHDNRTLWD L++  + +TD    +  DI +M KL AVI+FTSQG+PFIQ GQEM RTK D ENSY +PD +N I W  K++   +V+Y++GLI LR+AH ++F       I + + F + D G+ VPD+CI Y+L                         E+E   W  V+VLLN  P+ V+F +P       W  +V+   +G   I   I     V  RSAA+L
Sbjct:   68 LTFVLDKTHYGSGEMIGLLPRKG--EWLEKDDPDRIWTPNLGNEAWILQNDPDLFVEPPDTYP-------------------QISGAFIDAPRRVTVCLPISIRF-----------DPEQA-----EHVRIIDDQGQPIPIQSVA-------PGEIGNAALLLINTSDSFRAFDLAMPIHKYRVEVLGFKPKGLSFRMLMDRPEYVSDVELGAIYKPDQTLFRLFAPTATRVTLTIYDQPDRDIGQAY-PLESQASGVWEIDIKQNLKNKYYTYKVDGNSPLFHPDQELIDPYSRCNTAHNGRGMIID-DGTRIADRPDFPPEDAIIYELHVRDFSIDTDSGINQKGLYLGMTEQGATYKDQ----------------------------GEVKTGLDHLVEMGVNTVQIMPIQDFDNNESSEE-YNWGYMPVHFNSPDGWYATEQRNAKRVEEFKKLVDALHTRGIRVIMDVVYNHTAET----NPYVQYSFNGIAPGYYYRM----------------------RDSGSFWNGSGCGNEFRSEAPMVRKFILDSVAYWVNEYKVDGFRFDLMGLIDLETMTQITETLHEIDPNILVYGEPWASGETPISITSKGKQRGLGFGVFNDNFRDALKGSPFDRFDISETFLIHGSRISEIKKGIEGSIHSFAQRPLESVNYMECHDNRTLWDQLHYLTQGQTD---IDARDIVQMVKLGAVIIFTSQGVPFIQMGQEMLRTKHDVENSYNAPDSVNQIEWERKIRYRDVVEYHKGLIQLRKAH-QMFRQTDPQAIEKNLKFLDNDLGMTVPDKCIAYQL-------------------------EDERDEWERVLVLLNPNPISVIFPIP----PGTWTSVVDENQSGVEPIQIGIEREKRVAPRSAAVL 841          

BLAST of Ggra10630.t1 vs. uniprot
Match: A0A3M2EUP4 (Pullulanase n=1 Tax=Candidatus Hydrogenedentes bacterium TaxID=2030809 RepID=A0A3M2EUP4_9BACT)

HSP 1 Score: 608 bits (1569), Expect = 4.720e-197
Identity = 372/918 (40.52%), Postives = 500/918 (54.47%), Query Frame = 0

Query:  206 FIVDRACYGA-ANSICLLPRRGGDAWLEKDGTERVWNASFLEEQCVQSEDRPQYDDKYDHLPTFIITQSTSFVLRSLDDVKSMMNAYVDA----ENSIIVTTP----VPVEWISPPKTCGRHSNPEVVVQLCKRSVQALLDEGKQSHDQVAGKVLRYRKSKQLSLTKLRLLFDDSVAAFDEDFLVENVVVSIPPFDSVCLHWETHEDEDQYLYKGSLGWTYTEKQCVFRCFAPAADQVSVILYEKPTGDAGRVVVPMRRIPQGCWKATVLKNLQGRFYKLLAEGENKRLFPGVEVIDPYSKCNTAHAGRGLIYGFDTTPVCERPKVETAEIIVYELHVRDMTIDKLSGIRNSGKFLGLAERGTRMKMKEPENRPDKHLTLWEQEAMPGVETAFSMLDKFSTGLDHIAQMGVTAVQIMPVQDFDNNEADDEAYRWGYMPVHFNSPDGWYASSTYSSARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNEFNMEARFSFNGLAPRYYYRTCGNTPVAHNGESTCGRREPHEPRCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWVTEYGVDGFRFDLLGLIDVNTIQRAVEELKAIDSDIIVYGEPWIGGLSPIKATEKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFVMDGGRLTEVKGGIIGSVDMFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFNEEDIRRMHKLAAVIVFTSQGIPFIQAGQEMCRTKFDDENSYESPDEINMIRWSSKLKEWSIVQYYRGLILLRRAHPEIFCIETANEIHEKVVFYED-CGLRVPDRCIGYRLKGDPEKLLKRLQDTHHQAGWKGQDHEEESLRWSEVVVLLNATPLQVVFELPGKKGDVLWMQIVNAWTAGTRTINGPIIGS-VCVGGRSAAILRRASPQ 1112
            F +D+  YG+  + I LLP+ G   W  KD  +R W  S      + S D+  Y    D  P  +       +L  +++V+ +++  +D     + ++ V  P    VPV    P    G          L  R++   L + +   + VAG V+                   S  A+    L+   VV                  D Y Y G LG  Y+ ++ +FR F+P +  VSV++YE PTG   R VVPM+RI QG W+A V  NL  ++YK   +  +       EVIDPYS CNTAH GRG+I   D TPV   P+   A+ ++YE+H+RD TID  SGI++ GK+LGL E GTR+     E  P+                        STGLDHI ++GV  VQIMP QDFDN+EA  EAY WGYMP HFNSPDGWYA+    ++RV+E KK++ A H+ G++V+MDVVYNHTAE     N E R SFNGLAP YYYR      V  +G                 Y NGSG GNEFR+E+PM RKFI+DS+K+WV +Y VDGFRFDL+GLID+ T++  V EL+ I  +I++YGEPW GG +P+K T KG QR KGFAVFND FR+A++G  F+TE  F+ DG  + +VK GI+GS+D F D+P E+INYV  HDN TLWD +    R+ T   K   +D  +M KLAAV + T+QGIPF+  GQ++ RTK  +ENSY  PD INMI W  K K   + +YYRGLI LRRAHP +F   TA+ +   V F +D   LRVP R IG+ +                    +G+  +E    W  V+VL+NA+     FE+P       W+ + N   AG   +  P+  S V +  RS+AI     P+
Sbjct:  118 FRLDKNDYGSNVDRIGLLPKYGD--WKAKDDPDRYWTPSLGRNVFILSADKNLYSSPPDTRPRILRA-----LLDKVNEVRVVLSKSIDRSYVRKGTVSVRLPDRSAVPVVEAVPAPGQG----------LKVRTILVRLGQ-EIDPESVAGIVV-------------------SFGAYRPAPLIPGAVV------------------DSYRYDGELGALYSPEKTIFRTFSPTSAGVSVVIYEGPTGPP-RDVVPMKRIGQGIWEAIVEGNLLDKYYKYRVKYPDAEY----EVIDPYSHCNTAHNGRGMIID-DNTPVSPAPRFGIADAVIYEIHIRDFTIDPKSGIQHRGKYLGLTEEGTRL-----EGNPE-----------------------ISTGLDHIKELGVNTVQIMPFQDFDNDEAS-EAYNWGYMPFHFNSPDGWYATKRDDASRVREAKKMIDAFHRNGIKVVMDVVYNHTAEG----NPEVRMSFNGLAPNYYYR------VKDDGS----------------YWNGSGTGNEFRTEAPMARKFIVDSMKYWVRKYDVDGFRFDLMGLIDLETLETIVRELRKIKPEIMIYGEPWAGGSTPVKVTSKGDQRGKGFAVFNDHFRDALKGGTFNTEPGFIQDGRDVEKVKKGILGSIDDFTDAPTETINYVAAHDNHTLWDRILLTTRNWTQVTK---KDREQMDKLAAVAILTAQGIPFLHGGQDLLRTKDGEENSYNKPDRINMIHWEWKKKHREVFEYYRGLIALRRAHP-LFRKTTADAVRRGVFFLDDDLELRVPPRAIGFVVN-------------------RGEAQDE----WENVLVLMNASATSARFEIPASH----WIPVANGEKAGVTPLGPPVTRSEVILPPRSSAIFYNTDPE 888